Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0005981

Physical Location & Seq

Genomic Coordinates Reverse (-)

25742436 .. 25748876

Gene Structure 6441 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1199100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGAGTTCGAATTTGGATTTGGTACGCGCGTTGGAGTCGGCGCTGGGGGTTTCGTTCGGTGGTTCGGTGTCGGACACCGTGGTGGTGATTGCGACGACTTCAATTGCGCTGATAATTGGGGTTTTGTTGCTGGTGTGGAGGAGATCGTCGGATCGGAGCCGGGAGGTGAAGCAGCTGGTGGTGCCCAAGCCGCTTGAGGTGAAGGAGGAGGAAGACGAGTTTGAGGTTGCAGCGGGGAAGACTAGGGTCACGATCTTCTATGGAACTCAGACTGGTACAGCTGAGGGTTTCGCTAAGGCTTTGGCTGAGGAGATCAATGCTAGATATGAGAAAGCTGCTGTGAAGGTTGTCGACCTGGATGACTATGCTGAGGATGATGATACATATGAAGAAAAACTGAAGAAAGAGACTTTGGCATTTTTTATGTTGGCCACTTATGGAGATGGGGAGCCAACTGATAATGCAGCAAGATTTTACAAATGGTTTACGGAGGGAAAAGATAGGGGTAGCTGGCTTCAACATCTAAAATATGGTGTTTTTGGTCTGGGTAACCGACAATATGAACATTTCAATAAGATAGGAAAAGTGGTTGATGATCAACTTAGTGAAGAAGGTGCAAAGCGTGTTGTTCCTGTTGGCTTAGGTGATGATGATCAATGCATTGAGGACGATTTTTCTGCTTGGAGGGAATCATTGTGGCCTGAGTTGGATCTATTCCTCCGAGATGAAGATGATACAAATTCTGCAGCTACTCCATATACAGCTGTAATTCCAGAATATAGAGTTGTTATTCATGACCCTAGTGTTATTACCTCTTATGAGGACAGTCAGTCGAATATGGCTAATGGGAATGCGTCACTCGATATTCACCATCCTTGCAGAGTTAATGTTGCTGTTCAAAGAGAGCTTCACAAACCTGAGTCTGACCGCTCTTGCATCCATCTGGAGTTTGATATCTCTGGAACTGGTATTACATATGAAACTGGAGACCATGTGGGTGTTTATGCAGATAATTGTGATGAAACAGTTGAAGAAGCTGCAAGGTTGTTGGGCCAACCTTTAGATTTGCTATTCTCCATACATACTGACAATGATGATGGGACGTCTCTTGGAAGCTCATTACCGCCTCCGTTTCCTGGTCCGTGCACAGTCCGCACCACATTGGCACGTTATGCTGATCTATTGAACCCACCTCGGAAGGCTGCTCTACTTGCTTTGGCTGCACATGCCGTTGAACCGAGTGAAGCAGAAAGACTTAAATTTTTATCATCACCTCAGGGGAAGGATGAGTACTCAAAATGGATTGTTGGAAGTCAGAGAAGTCTTGTTGAGGTAATGGCTGAGTTCCCATCAGCGAAGCCTCCACTTGGTGTGTTTTTTGCTGCCGTAGCCCCTCGCTTACAGCCTCGTTACTATTCCATATCATCTTCACCTAGATTTGCTCCCCATCGTGTTCATGTAACCTGTGCTTTAGTTTATGGTCCAACGCCAACTGGCAGAATTCACAAAGGAGTGTGTTCGTTCTGGATGAAGAATGCAGTTCCTCTAGAGAAAAGCCATAACAGTAGCTGGGCTCCTATTTTTATTAGGCAATCCAATTTCAAGCTACCCGCTGATCCTTCAGTCCCTATTATCATGGTGGGGCCTGGTACGGGCTTCGCACCTTTCCGAGGATTTCTACAGGAAAGGATGGCCCTGAAAAGGGAGGGTGCTCAATTAGGGCCTGCACTTCTCTTCTTTGGTTGCAGAAACCGCAGAATGGATTTCATTTATGAAGATGAGTTAAATAACTATGTGGAAGAAGGTGTGTTGTCTGAACTAATTGTTGCATTCTCACGTGAGGGACCGACAAAGGAGTATGTTCAACATAAACTGATGGAGAAAGCAGCATACACGTGGGACTTAGTATCTCAAGGGGGATACTTTTATGTATGTGGTGACGCCAAAGGAATGGCTAGAGATGTTCATCGGACTTTGCATACCGTTGTCCAGGAGCAGGAAAGGGTGGACTCAACGAAGGCAGAAGCTAGAGTGAAACAACTTCAGATGGATGGAAGATACCTTAGAGATGTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006725 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009698 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0071704 GO:0097305 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

693

Amino Acids

77.18

Weight (kDa)

5.19

Isoelectric Point (pI)

39.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	85 - 228	3.6e-35	Flavodoxin
FAD_binding_1	PF00667	287 - 509	3.4e-75	FAD binding domain
NAD_binding_1	PF00175	546 - 656	9.4e-15	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005981)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24520 AT4G24520
fragaria_vesca	FvH4_5g34830 FvH4_5g34830 FvH4_5g34830 FvH4_5g34830 FvH4_5g34830
malus_domestica	MD08G1199100.v1.1 MD15G1386100.v1.1
prunus_persica	Prupe.1G532800_v2.0.a1 Prupe.1G532800_v2.0.a1 Prupe.1G532800_v2.0.a1
pyrus_communis	pycom08g17290 pycom15g34630
rosa_chinensis	RchiOBHm_Chr7g0235511
rosa_laevigata	RLG00000001153
rosa_multiflora	Rmu_sc0002617.1_g000018 Rmu_sc0002892.1_g000021 Rmu_sc0039003.1_g000003 Rmu_sc0042677.1_g000001
rosa_roxburghii	Rroxscaffold_3G00225940
rosa_rugosa	Rorug07G0291400
rosa_samantha	Rh7AG446500 Rh7BG418200 Rh7CG466500 Rh7DG435200
rosa_wichuraiana	Rw7G037070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1106
AccB1I	GGYRCC	1 cut(s)	181
AccBSI	CCGCTC	1 cut(s)	930
AccI	GTMKAC	1 cut(s)	351
AccII	CGCG	2 cut(s)	27, 29
AciI	CCGC	7 cut(s)	191, 233, 928, 1124, 1153, 1611, 1753
AclWI	GGATC	3 cut(s)	159, 717, 1610
AcoI	YGGCCR	1 cut(s)	429
AcsI	RAATTY	4 cut(s)	10, 739, 1259, 1500
AcuI	CTGAAG	3 cut(s)	419, 1605, 2027
AcvI	CACGTG	2 cut(s)	1838, 1896
AcyI	GRCGYC	2 cut(s)	1103, 1941
AdeI	CACNNNGTG	1 cut(s)	1370
AfaI	GTAC	4 cut(s)	24, 277, 1292, 1651
AfiI	CCNNNNNNNGG	8 cut(s)	1136, 1367, 1652, 1670, 1701, 1702, 1759, 1996
AflIII	ACRYGT	1 cut(s)	1893
AgsI	TTSAA	9 cut(s)	102, 518, 571, 899, 1031, 1186, 1235, 1603, 1865
AhdI	GACNNNNNGTC	1 cut(s)	829
AjnI	CCWGG	4 cut(s)	354, 1135, 1645, 1989
AjuI	GAANNNNNNNTTGG	3 cut(s)	34, 395, 427
AleI	CACNNNNGTG	1 cut(s)	80
Alw21I	GWGCWC	2 cut(s)	1148, 1714
Alw26I	GTCTC	3 cut(s)	401, 981, 1110
Alw44I	GTGCAC	1 cut(s)	1144
AlwI	GGATC	3 cut(s)	159, 717, 1610
AlwNI	CAGNNNCTG	1 cut(s)	1569
AoxI	GGCC	6 cut(s)	429, 698, 1051, 1643, 1692, 1721
ApaLI	GTGCAC	1 cut(s)	1144
ApoI	RAATTY	4 cut(s)	10, 739, 1259, 1500
AspLEI	GCGC	3 cut(s)	29, 43, 109
AspS9I	GGNCC	7 cut(s)	1051, 1139, 1481, 1643, 1693, 1721, 1844
AsuC2I	CCSGG	1 cut(s)	161
AsuHPI	GGTGA	8 cut(s)	97, 178, 211, 656, 860, 1263, 1422, 1949
AsuII	TTCGAA	1 cut(s)	8
AvaII	GGWCC	3 cut(s)	1139, 1481, 1844
AxyI	CCTNAGG	1 cut(s)	1275
BaeGI	GKGCMC	2 cut(s)	186, 1148
BaeI	ACNNNNGTAYC	2 cut(s)	14, 47
BalI	TGGCCA	1 cut(s)	431
BanI	GGYRCC	1 cut(s)	181
BanII	GRGCYC	1 cut(s)	1576
BarI	GAAGNNNNNNTAC	2 cut(s)	1274, 1306
BbrPI	CACGTG	2 cut(s)	1838, 1896
BbsI	GAAGAC	2 cut(s)	219, 245
Bbv12I	GWGCWC	2 cut(s)	1148, 1714
BbvCI	CCTCAGC	3 cut(s)	282, 306, 369
BceAI	ACGGC	2 cut(s)	1214, 1370
BcgI	CGANNNNNNTGC	2 cut(s)	659, 693
BciT130I	CCWGG	4 cut(s)	356, 1137, 1647, 1991
BciVI	GTATCC	1 cut(s)	1913
BclI	TGATCA	2 cut(s)	595, 652
BcnI	CCSGG	1 cut(s)	161
BcoDI	GTCTC	3 cut(s)	401, 981, 1110
BfaI	CTAG	7 cut(s)	243, 321, 801, 1434, 1547, 1956, 2028
BfmI	CTRYAG	2 cut(s)	744, 1679
BfoI	RGCGCY	1 cut(s)	44
BfuI	GTATCC	1 cut(s)	1913
BmcAI	AGTACT	1 cut(s)	1292
Bme1390I	CCNGG	5 cut(s)	161, 356, 1137, 1647, 1991
Bme18I	GGWCC	3 cut(s)	1139, 1481, 1844
BmeRI	GACNNNNNGTC	1 cut(s)	829
BmgT120I	GGNCC	7 cut(s)	1051, 1139, 1481, 1643, 1693, 1721, 1844
BmiI	GGNNCC	6 cut(s)	158, 183, 450, 1575, 1644, 1845
BmrFI	CCNGG	5 cut(s)	161, 356, 1137, 1647, 1991
BmsI	GCATC	1 cut(s)	945
BoxI	GACNNNNGTC	1 cut(s)	245
BpiI	GAAGAC	2 cut(s)	219, 245
BpmI	CTGGAG	2 cut(s)	965, 1005
Bpu10I	CCTNAGC	5 cut(s)	282, 294, 306, 369, 640
Bpu14I	TTCGAA	1 cut(s)	8
BpuEI	CTTGAG	2 cut(s)	215, 1896
BpuMI	CCSGG	1 cut(s)	161
BsaAI	YACGTR	2 cut(s)	1838, 1896
BsaBI	GATNNNNATC	2 cut(s)	651, 1965
BsaHI	GRCGYC	2 cut(s)	1103, 1941
BsaI	GGTCTC	1 cut(s)	981
BsaJI	CCNNGG	3 cut(s)	78, 1193, 1669
BsaXI	ACNNNNNCTCC	2 cut(s)	978, 1008
Bsc4I	CCNNNNNNNGG	8 cut(s)	1136, 1367, 1652, 1670, 1701, 1702, 1759, 1996
Bse1I	ACTGG	4 cut(s)	277, 970, 988, 1498
Bse21I	CCTNAGG	1 cut(s)	1275
Bse8I	GATNNNNATC	2 cut(s)	651, 1965
BseBI	CCWGG	4 cut(s)	356, 1137, 1647, 1991
BseDI	CCNNGG	3 cut(s)	78, 1193, 1669
BseGI	GGATG	8 cut(s)	364, 379, 871, 936, 1291, 1533, 1695, 2056
BseJI	GATNNNNATC	2 cut(s)	651, 1965
BseLI	CCNNNNNNNGG	8 cut(s)	1136, 1367, 1652, 1670, 1701, 1702, 1759, 1996
BseMII	CTCAG	8 cut(s)	273, 281, 297, 360, 693, 909, 1289, 1332
BseNI	ACTGG	4 cut(s)	277, 970, 988, 1498
BseRI	GAGGAG	3 cut(s)	154, 221, 323
BseSI	GKGCMC	2 cut(s)	186, 1148
BseYI	CCCAGC	2 cut(s)	43, 1569
BsgI	GTGCAG	2 cut(s)	1206, 1710
Bsh1236I	CGCG	2 cut(s)	27, 29
BshFI	GGCC	6 cut(s)	431, 700, 1053, 1645, 1694, 1723
BshNI	GGYRCC	1 cut(s)	181
BsiHKAI	GWGCWC	2 cut(s)	1148, 1714
BsiSI	CCGG	1 cut(s)	160
BslFI	GGGAC	4 cut(s)	1114, 1610, 1857, 1913
BslI	CCNNNNNNNGG	8 cut(s)	1136, 1367, 1652, 1670, 1701, 1702, 1759, 1996
BsmAI	GTCTC	3 cut(s)	401, 981, 1110
BsmBI	CGTCTC	1 cut(s)	1110
BsmFI	GGGAC	4 cut(s)	1114, 1610, 1857, 1913
BsmI	GAATGC	3 cut(s)	856, 1540, 1829
BsnI	GGCC	6 cut(s)	431, 700, 1053, 1645, 1694, 1723
Bso31I	GGTCTC	1 cut(s)	981
Bsp119I	TTCGAA	1 cut(s)	8
Bsp1286I	GDGCHC	4 cut(s)	186, 1148, 1576, 1714
Bsp143I	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
BspACI	CCGC	7 cut(s)	191, 233, 928, 1124, 1153, 1611, 1753
BspANI	GGCC	6 cut(s)	431, 700, 1053, 1645, 1694, 1723
BspCNI	CTCAG	8 cut(s)	274, 280, 298, 361, 694, 910, 1288, 1333
BspFNI	CGCG	2 cut(s)	27, 29
BspHI	TCATGA	1 cut(s)	793
BspLI	GGNNCC	6 cut(s)	158, 183, 450, 1575, 1644, 1845
BspMAI	CTGCAG	1 cut(s)	748
BspPI	GGATC	3 cut(s)	159, 717, 1610
BspT104I	TTCGAA	1 cut(s)	8
BspT107I	GGYRCC	1 cut(s)	181
BspTNI	GGTCTC	1 cut(s)	981
BsrBI	CCGCTC	1 cut(s)	930
BsrI	ACTGG	4 cut(s)	277, 970, 988, 1498
BssECI	CCNNGG	3 cut(s)	78, 1193, 1669
BssMI	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
BssNI	GRCGYC	2 cut(s)	1103, 1941
Bst2UI	CCWGG	4 cut(s)	356, 1137, 1647, 1991
Bst4CI	ACNGT	6 cut(s)	79, 827, 1027, 1150, 1565, 1984
Bst6I	CTCTTC	1 cut(s)	1739
BstACI	GRCGYC	2 cut(s)	1103, 1941
BstAPI	GCANNNNNTGC	1 cut(s)	1172
BstBAI	YACGTR	2 cut(s)	1838, 1896
BstBI	TTCGAA	1 cut(s)	8
BstC8I	GCNNGC	2 cut(s)	512, 1725
BstDSI	CCRYGG	1 cut(s)	78
BstEII	GGTNACC	1 cut(s)	548
BstENI	CCTNNNNNAGG	1 cut(s)	1668
BstF5I	GGATG	8 cut(s)	364, 379, 871, 936, 1291, 1533, 1695, 2056
BstFNI	CGCG	2 cut(s)	27, 29
BstH2I	RGCGCY	1 cut(s)	44
BstHHI	GCGC	3 cut(s)	29, 43, 109
BstKTI	GATC	9 cut(s)	146, 154, 255, 315, 598, 655, 712, 1180, 1618
BstMAI	GTCTC	3 cut(s)	401, 981, 1110
BstMBI	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
BstMWI	GCNNNNNNNGC	9 cut(s)	181, 190, 1152, 1172, 1211, 1220, 1226, 1388, 1752
BstNI	CCWGG	4 cut(s)	356, 1137, 1647, 1991
BstNSI	RCATGY	1 cut(s)	1229
BstPAI	GACNNNNGTC	1 cut(s)	245
BstPI	GGTNACC	1 cut(s)	548
BstSCI	CCNGG	5 cut(s)	159, 354, 1135, 1645, 1989
BstSFI	CTRYAG	2 cut(s)	744, 1679
BstSLI	GKGCMC	2 cut(s)	186, 1148
BstUI	CGCG	2 cut(s)	27, 29
BstV2I	GAAGAC	2 cut(s)	219, 245
BstX2I	RGATCY	1 cut(s)	709
BstXI	CCANNNNNNTGG	1 cut(s)	1951
BstYI	RGATCY	1 cut(s)	709
Bsu36I	CCTNAGG	1 cut(s)	1275
BsuI	GTATCC	1 cut(s)	1913
BsuRI	GGCC	6 cut(s)	431, 700, 1053, 1645, 1694, 1723
BtgI	CCRYGG	1 cut(s)	78
BtsCI	GGATG	8 cut(s)	364, 379, 871, 936, 1291, 1533, 1695, 2056
Cac8I	GCNNGC	2 cut(s)	512, 1725
CaiI	CAGNNNCTG	1 cut(s)	1569
CciI	TCATGA	1 cut(s)	793
CfoI	GCGC	3 cut(s)	29, 43, 109
Cfr13I	GGNCC	7 cut(s)	1051, 1139, 1481, 1643, 1693, 1721, 1844
CseI	GACGC	2 cut(s)	843, 1949
Csp6I	GTAC	4 cut(s)	23, 276, 1291, 1650
CviAII	CATG	5 cut(s)	794, 992, 1226, 1457, 1636
CviQI	GTAC	4 cut(s)	23, 276, 1291, 1650
DpnI	GATC	9 cut(s)	145, 153, 254, 314, 597, 654, 711, 1179, 1617
DpnII	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
DraIII	CACNNNGTG	1 cut(s)	1370
DriI	GACNNNNNGTC	1 cut(s)	829
EaeI	YGGCCR	1 cut(s)	429
Eam1104I	CTCTTC	1 cut(s)	1739
Eam1105I	GACNNNNNGTC	1 cut(s)	829
EarI	CTCTTC	1 cut(s)	1739
Eco24I	GRGCYC	1 cut(s)	1576
Eco31I	GGTCTC	1 cut(s)	981
Eco32I	GATATC	1 cut(s)	955
Eco47I	GGWCC	3 cut(s)	1139, 1481, 1844
Eco57I	CTGAAG	3 cut(s)	419, 1605, 2027
Eco72I	CACGTG	2 cut(s)	1838, 1896
Eco81I	CCTNAGG	1 cut(s)	1275
Eco91I	GGTNACC	1 cut(s)	548
EcoNI	CCTNNNNNAGG	1 cut(s)	1668
EcoO109I	RGGNCCY	2 cut(s)	1643, 1721
EcoO65I	GGTNACC	1 cut(s)	548
EcoRI	GAATTC	1 cut(s)	1500
EcoRII	CCWGG	4 cut(s)	354, 1135, 1645, 1989
EcoRV	GATATC	1 cut(s)	955
EcoT22I	ATGCAT	1 cut(s)	662
EcoT38I	GRGCYC	1 cut(s)	1576
Esp3I	CGTCTC	1 cut(s)	1110
FaeI	CATG	5 cut(s)	797, 995, 1229, 1460, 1639
FaqI	GGGAC	4 cut(s)	1114, 1610, 1857, 1913
FatI	CATG	5 cut(s)	793, 991, 1225, 1456, 1635
FauI	CCCGC	2 cut(s)	226, 1618
FauNDI	CATATG	2 cut(s)	385, 976
FbaI	TGATCA	2 cut(s)	595, 652
FblI	GTMKAC	1 cut(s)	351
FokI	GGATG	8 cut(s)	371, 386, 858, 923, 1298, 1540, 1702, 2063
FriOI	GRGCYC	1 cut(s)	1576
FspBI	CTAG	7 cut(s)	243, 321, 801, 1434, 1547, 1956, 2028
GlaI	GCGC	3 cut(s)	28, 42, 108
GsaI	CCCAGC	2 cut(s)	47, 1573
GsuI	CTGGAG	2 cut(s)	965, 1005
HaeII	RGCGCY	1 cut(s)	44
HaeIII	GGCC	6 cut(s)	431, 700, 1053, 1645, 1694, 1723
HapII	CCGG	1 cut(s)	160
HgaI	GACGC	2 cut(s)	843, 1949
HhaI	GCGC	3 cut(s)	29, 43, 109
Hin1I	GRCGYC	2 cut(s)	1103, 1941
Hin1II	CATG	5 cut(s)	797, 995, 1229, 1460, 1639
Hin6I	GCGC	3 cut(s)	27, 41, 107
HinP1I	GCGC	3 cut(s)	27, 41, 107
HincII	GTYRAC	1 cut(s)	352
HindII	GTYRAC	1 cut(s)	352
HinfI	GANTC	4 cut(s)	35, 689, 920, 2009
HpaII	CCGG	1 cut(s)	160
HphI	GGTGA	8 cut(s)	97, 178, 211, 656, 860, 1263, 1422, 1949
Hpy166II	GTNNAC	4 cut(s)	352, 486, 1146, 2008
Hpy188III	TCNNGA	7 cut(s)	250, 773, 794, 944, 960, 1525, 1547
Hpy8I	GTNNAC	4 cut(s)	352, 486, 1146, 2008
Hpy99I	CGWCG	2 cut(s)	97, 151
HpyAV	CCTTC	8 cut(s)	196, 337, 605, 1192, 1276, 1629, 1796, 2011
HpyCH4III	ACNGT	6 cut(s)	79, 827, 1027, 1150, 1565, 1984
HpyCH4IV	ACGT	4 cut(s)	1103, 1168, 1837, 1895
HpyF10VI	GCNNNNNNNGC	9 cut(s)	181, 190, 1152, 1172, 1211, 1220, 1226, 1388, 1752
HpySE526I	ACGT	4 cut(s)	1103, 1168, 1837, 1895
Hsp92I	GRCGYC	2 cut(s)	1103, 1941
Hsp92II	CATG	5 cut(s)	797, 995, 1229, 1460, 1639
HspAI	GCGC	3 cut(s)	27, 41, 107
Ksp22I	TGATCA	2 cut(s)	595, 652
Kzo9I	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
LmnI	GCTCC	5 cut(s)	156, 448, 1447, 1579, 1993
LweI	GCATC	1 cut(s)	945
MaeI	CTAG	7 cut(s)	243, 321, 801, 1434, 1547, 1956, 2028
MaeII	ACGT	4 cut(s)	1103, 1168, 1837, 1895
MaeIII	GTNAC	6 cut(s)	247, 548, 855, 1409, 1459, 1937
MalI	GATC	9 cut(s)	145, 153, 254, 314, 597, 654, 711, 1179, 1617
MbiI	CCGCTC	1 cut(s)	930
MboI	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
MfeI	CAATTG	1 cut(s)	102
MflI	RGATCY	1 cut(s)	709
MhlI	GDGCHC	4 cut(s)	186, 1148, 1576, 1714
MlsI	TGGCCA	1 cut(s)	431
MluNI	TGGCCA	1 cut(s)	431
MlyI	GAGTC	3 cut(s)	44, 929, 2003
MmeI	TCCRAC	6 cut(s)	12, 51, 129, 687, 1288, 1508
Mox20I	TGGCCA	1 cut(s)	431
Mph1103I	ATGCAT	1 cut(s)	662
MscI	TGGCCA	1 cut(s)	431
MseI	TTAA	3 cut(s)	885, 1257, 1784
MslI	CAYNNNNRTG	3 cut(s)	80, 996, 1894
Msp20I	TGGCCA	1 cut(s)	431
MspA1I	CMGCKG	5 cut(s)	175, 233, 281, 764, 1613
MspI	CCGG	1 cut(s)	160
MspR9I	CCNGG	5 cut(s)	161, 356, 1137, 1647, 1991
MunI	CAATTG	1 cut(s)	102
Mva1269I	GAATGC	3 cut(s)	856, 1540, 1829
MvaI	CCWGG	4 cut(s)	356, 1137, 1647, 1991
MvnI	CGCG	2 cut(s)	27, 29
MwoI	GCNNNNNNNGC	9 cut(s)	181, 190, 1152, 1172, 1211, 1220, 1226, 1388, 1752
NciI	CCSGG	1 cut(s)	161
NdeI	CATATG	2 cut(s)	385, 976
NdeII	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
NlaIII	CATG	5 cut(s)	797, 995, 1229, 1460, 1639
NlaIV	GGNNCC	6 cut(s)	158, 183, 450, 1575, 1644, 1845
NmuCI	GTSAC	3 cut(s)	247, 855, 1937
NsiI	ATGCAT	1 cut(s)	662
NspI	RCATGY	1 cut(s)	1229
NspV	TTCGAA	1 cut(s)	8
OliI	CACNNNNGTG	1 cut(s)	80
PagI	TCATGA	1 cut(s)	793
PctI	GAATGC	3 cut(s)	856, 1540, 1829
PfeI	GAWTC	1 cut(s)	689
PfoI	TCCNGGA	1 cut(s)	1989
PleI	GAGTC	3 cut(s)	43, 928, 2003
PmaCI	CACGTG	2 cut(s)	1838, 1896
PmlI	CACGTG	2 cut(s)	1838, 1896
PpsI	GAGTC	3 cut(s)	43, 928, 2003
Ppu21I	YACGTR	2 cut(s)	1838, 1896
PshAI	GACNNNNGTC	1 cut(s)	245
Psp6I	CCWGG	4 cut(s)	354, 1135, 1645, 1989
PspCI	CACGTG	2 cut(s)	1838, 1896
PspEI	GGTNACC	1 cut(s)	548
PspFI	CCCAGC	2 cut(s)	43, 1569
PspGI	CCWGG	4 cut(s)	354, 1135, 1645, 1989
PspN4I	GGNNCC	6 cut(s)	158, 183, 450, 1575, 1644, 1845
PspPI	GGNCC	7 cut(s)	1051, 1139, 1481, 1643, 1693, 1721, 1844
PsrI	GAACNNNNNNTAC	2 cut(s)	955, 987
PstI	CTGCAG	1 cut(s)	748
PstNI	CAGNNNCTG	1 cut(s)	1569
PsuI	RGATCY	1 cut(s)	709
PvuII	CAGCTG	3 cut(s)	175, 281, 764
RsaI	GTAC	4 cut(s)	24, 277, 1292, 1651
RsaNI	GTAC	4 cut(s)	23, 276, 1291, 1650
RseI	CAYNNNNRTG	3 cut(s)	80, 996, 1894
SalI	GTCGAC	1 cut(s)	350
SaqAI	TTAA	3 cut(s)	885, 1257, 1784
Sau3AI	GATC	9 cut(s)	143, 151, 252, 312, 595, 652, 709, 1177, 1615
Sau96I	GGNCC	7 cut(s)	1051, 1139, 1481, 1643, 1693, 1721, 1844
ScaI	AGTACT	1 cut(s)	1292
SchI	GAGTC	3 cut(s)	44, 929, 2003
ScrFI	CCNGG	5 cut(s)	161, 356, 1137, 1647, 1991
SduI	GDGCHC	4 cut(s)	186, 1148, 1576, 1714
SfaNI	GCATC	1 cut(s)	945
SfcI	CTRYAG	2 cut(s)	744, 1679
SfuI	TTCGAA	1 cut(s)	8
SinI	GGWCC	3 cut(s)	1139, 1481, 1844
SmiMI	CAYNNNNRTG	3 cut(s)	80, 996, 1894
SmlI	CTYRAG	2 cut(s)	194, 1911
SmoI	CTYRAG	2 cut(s)	194, 1911
SsiI	CCGC	7 cut(s)	191, 233, 928, 1124, 1153, 1611, 1753
SspMI	CTAG	7 cut(s)	243, 321, 801, 1434, 1547, 1956, 2028
StyD4I	CCNGG	5 cut(s)	159, 354, 1135, 1645, 1989
TaaI	ACNGT	6 cut(s)	79, 827, 1027, 1150, 1565, 1984
TaiI	ACGT	4 cut(s)	1106, 1171, 1840, 1898
TaqI	TCGA	4 cut(s)	8, 351, 833, 861
TaqII	GACCGA	1 cut(s)	1861
TatI	WGTACW	1 cut(s)	1290
TauI	GCSGC	1 cut(s)	193
TfiI	GAWTC	1 cut(s)	689
Tru1I	TTAA	3 cut(s)	885, 1257, 1784
Tru9I	TTAA	3 cut(s)	885, 1257, 1784
TseFI	GTSAC	3 cut(s)	247, 855, 1937
Tsp45I	GTSAC	3 cut(s)	247, 855, 1937
TspGWI	ACGGA	3 cut(s)	503, 1119, 1131
VneI	GTGCAC	1 cut(s)	1144
VpaK11BI	GGWCC	3 cut(s)	1139, 1481, 1844
XagI	CCTNNNNNAGG	1 cut(s)	1668
XapI	RAATTY	4 cut(s)	10, 739, 1259, 1500
XbaI	TCTAGA	1 cut(s)	1546
XceI	RCATGY	1 cut(s)	1229
XmiI	GTMKAC	1 cut(s)	351
XspI	CTAG	7 cut(s)	243, 321, 801, 1434, 1547, 1956, 2028
ZraI	GACGTC	1 cut(s)	1104
ZrmI	AGTACT	1 cut(s)	1292
Zsp2I	ATGCAT	1 cut(s)	662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.