Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004961

Physical Location & Seq

Genomic Coordinates Reverse (-)

12903180 .. 12907596

Gene Structure 4417 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G25160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGAGTAGATCACCAGACAAGCTTGCTCTTCCTCCTCCTCCTCCTCCTCCCCCATCTCGTACCGTCGTTGTGGCTCTAAGTGGGAGCAGTAAAAGCAAATATGTAGTTACATGGGCGATCGAAAAATTTGCTACTGAAGGAAATGTTGGCTTCAAGCTGCTTCACATCCATCCAATGATTACTTCTGTACCTACACCAATGGGAAATGCAATCCCGATTTCCGAGGTACGAGATGATGTAGTAACTGCATATAGACAGGAAATACTGTGGCAGTCTGAAGAAATGCTTAAACCTTACACAAAACTGTTCGTGCGGAGAAAGGTTGCAGTAGAAGTTCTTGTGATCGAATCAGATAATGTGGCAGCCGCAATAGCTGAAGAAGTGACTCGGGATTCAATAGACAGAATTGTCATTGGAGGCTCATCCCGTAGTTTTTTCTCAAGGAAAGCTGATATTTGCTCGGTGATATCAGCTTTGATGCCAAACTTTTGTACAGTCTATGTTGTTTCAAAAGGTAAATTGTCATGCGTCCGACCATCAGACTCAGACGGAAATGCTACTATAAGAGAAGATGGTAGTGAGAGAACAAATTCTTCAAGCGGTTCTTCTGGTCCCACCTCAGATTCATCAGATGTAATGTCAAGTGCGCATGACTCTCAATCTCGTCCTCTATCCCTTCCGGTCAGGAGAATGCAACATTTCCCTGCAATTGCAGGACAGGCATCAGTTCCAATGGAAACGAGTTCTGTTGGCTCGGATGAAACCAGGTGCATGTCTTTGGATGCAGAGGAAGCTAGAGATGTTTCAAGTATCAATAGAAGCAGCACAGATACTACATCACGTTGGACTCCTCGGCGTAGAGATTATGAGGAGAGAAAAGAAGCTATGAGTTCTTCTTCGAGCAACCGTGAATACGGGAATTTTGGTACTAGGTTTAGTTGGAGTGGCATGGGTGTCGATACTACACACTCTCGGGCTTCTCAACAAGCTTCCAACATGTCTGATGCTCTAAGTGAACAATCCTATACAGACAACCAGGTAAACCTAAACTTTGAGGTTGAGAAGTTGAGAGCTGAGCTTAGACACGTTCAAGAAATGTATGCTGTGGCTCAAACCGAAACCTTTGATGCTTCTCGAAAGCTTGGTGAACTTAACCAACGTCGGTTAGAAGAAGCCATAAAGCTTGAAGAGCTAAAGCTGAAGGAGTATGAAGCTCGAGAGTTAGCAGAAAAGGAAAAGCAGAATTTTGAGAAGGCAAGGAGAGATGCAGAGAGCATGAGAGAAAGAGCAGAGAGAGAAATTGCGCAGAGAAGAGAAGCCGAAAGGAAATCCGCACGTGATACTAAAGAGAAAGAGAAGCTCGAGGGCACTCTCGGGTCTCCTCAGCTGCAATATCAGCACTTTGCTTGGGAAGAAATTATGGCTGCCACTTCATCATTCTCAGAAGAACTGAAGATCGGAATGGGAGCTTACGGAGCTGTTTACAAGTGTAATCTGCATCATACAACTGCAGTTGTCAAAGTTTTGCAGTCAGCTGAAAATCAACTTTCCAAACAATTCCAACAAGAGCTCGAAATCTTGAGCAAGATTAGGCATCCACATTTGGTTCTCCTTCTAGGAGCGTGCCCGGAGCAAGGTGCTTTAGTTTATGAGTACATGGAAAACGGTAGCTTGGAGGACAGACTTTTCCAGGTCAACAACAGTCCGCCACTCCCGTGGTTTGAGCGGTTTAGGATTGCTTGGGAAGTTGCAGCGGCTCTTGTCTTCCTCCACAAATCAAAGCCTAAACCAATCATTCACCGTGATCTTAAACCTGCAAACATCTTACTTGATCACAACTTTGTCAGCAAAGTTGGAGACGTGGGACTCTCAACGATGGTTCAAGTCGATCCTCTATCAACTAAATTCACTATATACAAACAAACAAGCCCCGTGGGAACGTTATGCTATATTGATCCGGAATATCAGCGCACTGGAAGGATATCATCGAAATCAGATATCTACTCCTTTGGAATGATTCTTCTTCAGCTTCTCACAGCAAAACCTGCTATTGCATTGACACATTTCGTTGAGAGCGCAATGGATAGTAATGATGAGTTCCTTAAGATCTTAGATCAGAAAGCCGGTAACTGGCCGATTGAAGAAACCCGGGAATTGGCCGCATTAGCTTTGTGTTGTACAGAGCTACGCGGGAAAGATAGACCTGATTTGAAAGATCAGATTCTTCCAGCGCTCGAAAACCTAAAGAAAGTAGCTGAAAAGGCAAGGAACTCATTTTCCGGCGTATCGACACAGCCTCCCACTCATTTCATCTGCCCATTACTTAAGGACGTGATGAACGAGCCGTGCGTTGCAGCAGACGGATACACATACGACCGTCATGCGATAGAGGAGTGGCTTAAGGAGCACAACACATCGCCGATGACGGATTCACCGTTGCATAGCAAGAACCTTTTACCCAATTATACTCTTTACACAGCCATCATGGAATGGAGGTCTACACGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

835

Amino Acids

93.75

Weight (kDa)

6.1

Isoelectric Point (pI)

50.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Usp	PF00582	20 - 155	5.2e-08	Universal stress protein family
Pkinase	PF00069	482 - 737	4e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	485 - 739	1.9e-38	Protein tyrosine and serine/threonine kinase
U-box	PF04564	767 - 834	3.4e-17	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25160 AT5G61550 AT5G61550 AT5G61550 AT5G61550
fragaria_vesca	FvH4_7g27030
malus_domestica	MD01G1177900.v1.1 MD07G1249700.v1.1
prunus_persica	Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1
pyrus_communis	pycom01g18870 pycom07g22310
rosa_chinensis	RchiOBHm_Chr1g0373831
rosa_laevigata	RLG00000026802
rosa_multiflora	Rmu_sc0013735.1_g000010 Rmu_ssc0000206.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283970
rosa_rugosa	Rorug01G0379500
rosa_samantha	Rh1AG389100 Rh1BG353300 Rh1CG366600 Rh1DG384300
rosa_wichuraiana	Rw1G034610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	407
Acc16I	TGCGCA	2 cut(s)	648, 1305
Acc36I	ACCTGC	2 cut(s)	1822, 2053
AccBSI	CCGCTC	1 cut(s)	1726
AccI	GTMKAC	1 cut(s)	2498
AccII	CGCG	1 cut(s)	2190
AccIII	TCCGGA	1 cut(s)	1957
AciI	CCGC	9 cut(s)	313, 366, 600, 1332, 1706, 1726, 1754, 2160, 2190
AclI	AACGTT	1 cut(s)	1940
AclWI	GGATC	2 cut(s)	1883, 1949
AcoI	YGGCCR	2 cut(s)	2132, 2157
AcsI	RAATTY	5 cut(s)	125, 589, 919, 1243, 1904
AcuI	CTGAAG	6 cut(s)	156, 297, 396, 1220, 1472, 2009
AcvI	CACGTG	1 cut(s)	1337
AfaI	GTAC	7 cut(s)	61, 189, 228, 493, 928, 1655, 2179
AfeI	AGCGCT	1 cut(s)	2232
AfiI	CCNNNNNNNGG	3 cut(s)	1634, 2130, 2155
AflII	CTTAAG	3 cut(s)	2102, 2324, 2399
AflIII	ACRYGT	2 cut(s)	996, 1084
AjiI	CACGTC	2 cut(s)	1861, 2332
AjnI	CCWGG	3 cut(s)	764, 1035, 1689
AjuI	GAANNNNNNNTTGG	4 cut(s)	129, 161, 166, 198
AleI	CACNNNNGTG	1 cut(s)	1714
AloI	GAACNNNNNNTCC	2 cut(s)	2261, 2293
Alw21I	GWGCWC	2 cut(s)	1572, 2409
Alw26I	GTCTC	2 cut(s)	1383, 1851
AlwI	GGATC	2 cut(s)	1883, 1949
Ama87I	CYCGRG	6 cut(s)	387, 972, 1215, 1361, 1373, 2148
Aor13HI	TCCGGA	1 cut(s)	1957
Aor51HI	AGCGCT	1 cut(s)	2232
AoxI	GGCC	2 cut(s)	2132, 2157
ApeKI	GCWGC	7 cut(s)	157, 362, 822, 1387, 1424, 1751, 2354
ApoI	RAATTY	5 cut(s)	125, 589, 919, 1243, 1904
Asp700I	GAANNNNTTC	1 cut(s)	589
AspLEI	GCGC	5 cut(s)	649, 1306, 1971, 2078, 2233
AspS9I	GGNCC	1 cut(s)	611
AsuC2I	CCSGG	3 cut(s)	1628, 2149, 2150
AsuHPI	GGTGA	5 cut(s)	3, 475, 1157, 1790, 2424
AvaI	CYCGRG	6 cut(s)	387, 972, 1215, 1361, 1373, 2148
AvaII	GGWCC	1 cut(s)	611
BaeGI	GKGCMC	2 cut(s)	1370, 1628
BaeI	ACNNNNGTAYC	2 cut(s)	171, 204
BanII	GRGCYC	1 cut(s)	1572
BbrPI	CACGTG	1 cut(s)	1337
BbsI	GAAGAC	1 cut(s)	1756
Bbv12I	GWGCWC	2 cut(s)	1572, 2409
BbvCI	CCTCAGC	1 cut(s)	1383
BbvI	GCAGC	7 cut(s)	144, 374, 834, 1374, 1411, 1763, 2366
BccI	CCATC	6 cut(s)	61, 177, 544, 566, 1870, 2489
BceAI	ACGGC	1 cut(s)	2329
BcgI	CGANNNNNNTGC	6 cut(s)	110, 144, 937, 971, 2363, 2397
BciT130I	CCWGG	3 cut(s)	766, 1037, 1691
BciVI	GTATCC	1 cut(s)	2357
BclI	TGATCA	1 cut(s)	1831
BcnI	CCSGG	3 cut(s)	1628, 2149, 2150
BcoDI	GTCTC	2 cut(s)	1383, 1851
BfaI	CTAG	3 cut(s)	795, 930, 1616
BfmI	CTRYAG	1 cut(s)	1509
BfoI	RGCGCY	1 cut(s)	2234
BfrI	CTTAAG	3 cut(s)	2102, 2324, 2399
BfuAI	ACCTGC	2 cut(s)	1822, 2053
BfuI	GTATCC	1 cut(s)	2357
BglII	AGATCT	1 cut(s)	2106
BlpI	GCTNAGC	1 cut(s)	1074
Bme1390I	CCNGG	6 cut(s)	766, 1037, 1628, 1691, 2149, 2150
Bme18I	GGWCC	1 cut(s)	611
BmeT110I	CYCGRG	6 cut(s)	387, 972, 1215, 1361, 1373, 2148
BmgBI	CACGTC	2 cut(s)	1861, 2332
BmgT120I	GGNCC	1 cut(s)	611
BmiI	GGNNCC	1 cut(s)	613
BmrFI	CCNGG	6 cut(s)	766, 1037, 1628, 1691, 2149, 2150
BmsI	GCATC	8 cut(s)	468, 731, 772, 994, 1117, 1255, 1507, 1603
BpiI	GAAGAC	1 cut(s)	1756
Bpu10I	CCTNAGC	1 cut(s)	1383
Bpu1102I	GCTNAGC	1 cut(s)	1074
BpuEI	CTTGAG	2 cut(s)	424, 1600
BpuMI	CCSGG	3 cut(s)	1628, 2149, 2150
BsaAI	YACGTR	1 cut(s)	1337
BsaI	GGTCTC	1 cut(s)	1383
BsaJI	CCNNGG	5 cut(s)	222, 851, 1715, 1932, 2148
BsaWI	WCCGGW	2 cut(s)	679, 1957
BsaXI	ACNNNNNCTCC	2 cut(s)	2281, 2311
Bsc4I	CCNNNNNNNGG	3 cut(s)	1634, 2130, 2155
Bse118I	RCCGGY	1 cut(s)	2123
Bse1I	ACTGG	2 cut(s)	1978, 2135
Bse3DI	GCAATG	1 cut(s)	2085
BseAI	TCCGGA	1 cut(s)	1957
BseBI	CCWGG	3 cut(s)	766, 1037, 1691
BseDI	CCNNGG	5 cut(s)	222, 851, 1715, 1932, 2148
BseGI	GGATG	6 cut(s)	165, 169, 422, 763, 787, 1594
BseLI	CCNNNNNNNGG	3 cut(s)	1634, 2130, 2155
BseMI	GCAATG	1 cut(s)	2085
BseMII	CTCAG	5 cut(s)	558, 633, 1065, 1397, 1455
BseNI	ACTGG	2 cut(s)	1978, 2135
BseRI	GAGGAG	9 cut(s)	24, 27, 30, 33, 36, 840, 884, 1371, 2405
BseSI	GKGCMC	2 cut(s)	1370, 1628
BseXI	GCAGC	7 cut(s)	144, 374, 834, 1374, 1411, 1763, 2366
Bsh1236I	CGCG	1 cut(s)	2190
Bsh1285I	CGRYCG	2 cut(s)	120, 2377
BshFI	GGCC	2 cut(s)	2134, 2159
BsiEI	CGRYCG	2 cut(s)	120, 2377
BsiHKAI	GWGCWC	2 cut(s)	1572, 2409
BsiHKCI	CYCGRG	6 cut(s)	387, 972, 1215, 1361, 1373, 2148
BsiSI	CCGG	6 cut(s)	680, 1628, 1958, 2124, 2149, 2280
BslFI	GGGAC	2 cut(s)	597, 1878
BslI	CCNNNNNNNGG	3 cut(s)	1634, 2130, 2155
BsmAI	GTCTC	2 cut(s)	1383, 1851
BsmBI	CGTCTC	1 cut(s)	1851
BsmFI	GGGAC	2 cut(s)	597, 1878
BsmI	GAATGC	1 cut(s)	696
BsnI	GGCC	2 cut(s)	2134, 2159
Bso31I	GGTCTC	1 cut(s)	1383
BsoBI	CYCGRG	6 cut(s)	387, 972, 1215, 1361, 1373, 2148
Bsp1286I	GDGCHC	4 cut(s)	1370, 1572, 1628, 2409
Bsp13I	TCCGGA	1 cut(s)	1957
Bsp1407I	TGTACA	2 cut(s)	491, 2177
Bsp1720I	GCTNAGC	1 cut(s)	1074
BspACI	CCGC	9 cut(s)	313, 366, 600, 1332, 1706, 1726, 1754, 2160, 2190
BspANI	GGCC	2 cut(s)	2134, 2159
BspCNI	CTCAG	5 cut(s)	557, 632, 1066, 1396, 1454
BspEI	TCCGGA	1 cut(s)	1957
BspFNI	CGCG	1 cut(s)	2190
BspLI	GGNNCC	1 cut(s)	613
BspMAI	CTGCAG	1 cut(s)	1513
BspMI	ACCTGC	2 cut(s)	1822, 2053
BspPI	GGATC	2 cut(s)	1883, 1949
BspQI	GCTCTTC	2 cut(s)	33, 1182
BspTI	CTTAAG	3 cut(s)	2102, 2324, 2399
BspTNI	GGTCTC	1 cut(s)	1383
BsrBI	CCGCTC	1 cut(s)	1726
BsrDI	GCAATG	1 cut(s)	2085
BsrFI	RCCGGY	1 cut(s)	2123
BsrGI	TGTACA	2 cut(s)	491, 2177
BsrI	ACTGG	2 cut(s)	1978, 2135
BssAI	RCCGGY	1 cut(s)	2123
BssECI	CCNNGG	5 cut(s)	222, 851, 1715, 1932, 2148
Bst2UI	CCWGG	3 cut(s)	766, 1037, 1691
Bst6I	CTCTTC	3 cut(s)	33, 1182, 1306
BstAFI	CTTAAG	3 cut(s)	2102, 2324, 2399
BstAPI	GCANNNNNTGC	1 cut(s)	2045
BstAUI	TGTACA	2 cut(s)	491, 2177
BstBAI	YACGTR	1 cut(s)	1337
BstC8I	GCNNGC	2 cut(s)	24, 1624
BstDEI	CTNAG	9 cut(s)	77, 544, 619, 1010, 1074, 1079, 1383, 1441, 2110
BstDSI	CCRYGG	2 cut(s)	1715, 1932
BstF5I	GGATG	6 cut(s)	165, 169, 422, 763, 787, 1594
BstFNI	CGCG	1 cut(s)	2190
BstH2I	RGCGCY	1 cut(s)	2234
BstHHI	GCGC	5 cut(s)	649, 1306, 1971, 2078, 2233
BstMAI	GTCTC	2 cut(s)	1383, 1851
BstMCI	CGRYCG	2 cut(s)	120, 2377
BstNI	CCWGG	3 cut(s)	766, 1037, 1691
BstNSI	RCATGY	2 cut(s)	775, 1000
BstSCI	CCNGG	6 cut(s)	764, 1035, 1626, 1689, 2147, 2148
BstSFI	CTRYAG	1 cut(s)	1509
BstSLI	GKGCMC	2 cut(s)	1370, 1628
BstUI	CGCG	1 cut(s)	2190
BstV1I	GCAGC	7 cut(s)	144, 374, 834, 1374, 1411, 1763, 2366
BstV2I	GAAGAC	1 cut(s)	1756
BstX2I	RGATCY	1 cut(s)	2106
BstXI	CCANNNNNNTGG	1 cut(s)	1716
BstYI	RGATCY	1 cut(s)	2106
BsuI	GTATCC	1 cut(s)	2357
BsuRI	GGCC	2 cut(s)	2134, 2159
BtgI	CCRYGG	2 cut(s)	1715, 1932
BtgZI	GCGATG	1 cut(s)	2400
BtrI	CACGTC	2 cut(s)	1861, 2332
BtsCI	GGATG	6 cut(s)	165, 169, 422, 763, 787, 1594
BtsIMutI	CAGTG	1 cut(s)	1971
BveI	ACCTGC	2 cut(s)	1822, 2053
Cac8I	GCNNGC	2 cut(s)	24, 1624
CfoI	GCGC	5 cut(s)	649, 1306, 1971, 2078, 2233
Cfr10I	RCCGGY	1 cut(s)	2123
Cfr13I	GGNCC	1 cut(s)	611
Cfr9I	CCCGGG	1 cut(s)	2148
CseI	GACGC	1 cut(s)	517
CsiI	ACCWGGT	2 cut(s)	764, 1035
Csp6I	GTAC	7 cut(s)	60, 188, 227, 492, 927, 1654, 2178
CspCI	CAANNNNNGTGG	2 cut(s)	1914, 1949
CviQI	GTAC	7 cut(s)	60, 188, 227, 492, 927, 1654, 2178
DdeI	CTNAG	9 cut(s)	77, 544, 619, 1010, 1074, 1079, 1383, 1441, 2110
DrdI	GACNNNNNNGTC	1 cut(s)	407
DseDI	GACNNNNNNGTC	1 cut(s)	407
EaeI	YGGCCR	2 cut(s)	2132, 2157
Eam1104I	CTCTTC	3 cut(s)	33, 1182, 1306
EarI	CTCTTC	3 cut(s)	33, 1182, 1306
EciI	GGCGGA	1 cut(s)	1695
Ecl136II	GAGCTC	1 cut(s)	1570
Eco24I	GRGCYC	1 cut(s)	1572
Eco31I	GGTCTC	1 cut(s)	1383
Eco32I	GATATC	3 cut(s)	468, 1983, 1999
Eco47I	GGWCC	1 cut(s)	611
Eco47III	AGCGCT	1 cut(s)	2232
Eco53kI	GAGCTC	1 cut(s)	1570
Eco57I	CTGAAG	6 cut(s)	156, 297, 396, 1220, 1472, 2009
Eco72I	CACGTG	1 cut(s)	1337
Eco88I	CYCGRG	6 cut(s)	387, 972, 1215, 1361, 1373, 2148
EcoICRI	GAGCTC	1 cut(s)	1570
EcoRII	CCWGG	3 cut(s)	764, 1035, 1689
EcoRV	GATATC	3 cut(s)	468, 1983, 1999
EcoT38I	GRGCYC	1 cut(s)	1572
Esp3I	CGTCTC	1 cut(s)	1851
FalI	AAGNNNNNCTT	4 cut(s)	270, 302, 589, 621
FaqI	GGGAC	2 cut(s)	597, 1878
FauI	CCCGC	1 cut(s)	2183
FbaI	TGATCA	1 cut(s)	1831
FblI	GTMKAC	1 cut(s)	2498
FokI	GGATG	6 cut(s)	152, 156, 409, 770, 794, 1581
FriOI	GRGCYC	1 cut(s)	1572
FspAI	RTGCGCAY	1 cut(s)	648
FspBI	CTAG	3 cut(s)	795, 930, 1616
FspI	TGCGCA	2 cut(s)	648, 1305
GlaI	GCGC	5 cut(s)	648, 1305, 1970, 2077, 2232
HaeII	RGCGCY	1 cut(s)	2234
HaeIII	GGCC	2 cut(s)	2134, 2159
HapII	CCGG	6 cut(s)	680, 1628, 1958, 2124, 2149, 2280
HgaI	GACGC	1 cut(s)	517
HhaI	GCGC	5 cut(s)	649, 1306, 1971, 2078, 2233
Hin6I	GCGC	5 cut(s)	647, 1304, 1969, 2076, 2231
HinP1I	GCGC	5 cut(s)	647, 1304, 1969, 2076, 2231
HincII	GTYRAC	1 cut(s)	1696
HindII	GTYRAC	1 cut(s)	1696
HindIII	AAGCTT	4 cut(s)	20, 987, 1139, 1181
HpaII	CCGG	6 cut(s)	680, 1628, 1958, 2124, 2149, 2280
HphI	GGTGA	5 cut(s)	3, 475, 1157, 1790, 2424
Hpy166II	GTNNAC	6 cut(s)	1016, 1042, 1148, 1483, 1696, 2499
Hpy188III	TCNNGA	8 cut(s)	214, 389, 685, 1091, 1134, 1217, 1579, 1958
Hpy8I	GTNNAC	6 cut(s)	1016, 1042, 1148, 1483, 1696, 2499
Hpy99I	CGWCG	2 cut(s)	68, 1164
HpyAV	CCTTC	6 cut(s)	131, 686, 1195, 1246, 1622, 1971
HpyCH4IV	ACGT	7 cut(s)	841, 1086, 1159, 1336, 1860, 1940, 2331
HpyF3I	CTNAG	9 cut(s)	77, 544, 619, 1010, 1074, 1079, 1383, 1441, 2110
HpySE526I	ACGT	7 cut(s)	841, 1086, 1159, 1336, 1860, 1940, 2331
HspAI	GCGC	5 cut(s)	647, 1304, 1969, 2076, 2231
Kpn2I	TCCGGA	1 cut(s)	1957
Ksp22I	TGATCA	1 cut(s)	1831
LguI	GCTCTTC	2 cut(s)	33, 1182
LmnI	GCTCC	6 cut(s)	84, 1466, 1475, 1619, 1630, 2404
Lsp1109I	GCAGC	7 cut(s)	144, 374, 834, 1374, 1411, 1763, 2366
LweI	GCATC	8 cut(s)	468, 731, 772, 994, 1117, 1255, 1507, 1603
MabI	ACCWGGT	2 cut(s)	764, 1035
MaeI	CTAG	3 cut(s)	795, 930, 1616
MaeII	ACGT	7 cut(s)	841, 1086, 1159, 1336, 1860, 1940, 2331
MaeIII	GTNAC	4 cut(s)	106, 241, 382, 2126
MbiI	CCGCTC	1 cut(s)	1726
MfeI	CAATTG	1 cut(s)	708
MflI	RGATCY	1 cut(s)	2106
MhlI	GDGCHC	4 cut(s)	1370, 1572, 1628, 2409
MlyI	GAGTC	5 cut(s)	379, 536, 647, 841, 1860
MmeI	TCCRAC	6 cut(s)	556, 824, 920, 1017, 1585, 1834
MroI	TCCGGA	1 cut(s)	1957
MroXI	GAANNNNTTC	1 cut(s)	589
MseI	TTAA	6 cut(s)	288, 1152, 1809, 2103, 2325, 2400
MslI	CAYNNNNRTG	1 cut(s)	1714
MspA1I	CMGCKG	3 cut(s)	1387, 1535, 1754
MspCI	CTTAAG	3 cut(s)	2102, 2324, 2399
MspI	CCGG	6 cut(s)	680, 1628, 1958, 2124, 2149, 2280
MspR9I	CCNGG	6 cut(s)	766, 1037, 1628, 1691, 2149, 2150
MunI	CAATTG	1 cut(s)	708
Mva1269I	GAATGC	1 cut(s)	696
MvaI	CCWGG	3 cut(s)	766, 1037, 1691
MvnI	CGCG	1 cut(s)	2190
NciI	CCSGG	3 cut(s)	1628, 2149, 2150
NlaIV	GGNNCC	1 cut(s)	613
NmeAIII	GCCGAG	1 cut(s)	832
NmuCI	GTSAC	1 cut(s)	382
NsbI	TGCGCA	2 cut(s)	648, 1305
NspI	RCATGY	2 cut(s)	775, 1000
OliI	CACNNNNGTG	1 cut(s)	1714
PaeR7I	CTCGAG	2 cut(s)	1215, 1361
PciI	ACATGT	1 cut(s)	996
PciSI	GCTCTTC	2 cut(s)	33, 1182
PcsI	WCGNNNNNNNCGW	3 cut(s)	2337, 2346, 2432
PctI	GAATGC	1 cut(s)	696
PdmI	GAANNNNTTC	1 cut(s)	589
PfeI	GAWTC	6 cut(s)	347, 392, 623, 2017, 2221, 2429
Ple19I	CGATCG	1 cut(s)	120
PleI	GAGTC	5 cut(s)	379, 536, 647, 841, 1860
PmaCI	CACGTG	1 cut(s)	1337
PmlI	CACGTG	1 cut(s)	1337
PpsI	GAGTC	5 cut(s)	379, 536, 647, 841, 1860
Ppu21I	YACGTR	1 cut(s)	1337
PscI	ACATGT	1 cut(s)	996
Psp124BI	GAGCTC	1 cut(s)	1572
Psp1406I	AACGTT	1 cut(s)	1940
Psp6I	CCWGG	3 cut(s)	764, 1035, 1689
PspCI	CACGTG	1 cut(s)	1337
PspGI	CCWGG	3 cut(s)	764, 1035, 1689
PspN4I	GGNNCC	1 cut(s)	613
PspPI	GGNCC	1 cut(s)	611
PspXI	VCTCGAGB	1 cut(s)	1361
PstI	CTGCAG	1 cut(s)	1513
PsuI	RGATCY	1 cut(s)	2106
PvuI	CGATCG	1 cut(s)	120
PvuII	CAGCTG	2 cut(s)	1387, 1535
RsaI	GTAC	7 cut(s)	61, 189, 228, 493, 928, 1655, 2179
RsaNI	GTAC	7 cut(s)	60, 188, 227, 492, 927, 1654, 2178
RseI	CAYNNNNRTG	1 cut(s)	1714
SacI	GAGCTC	1 cut(s)	1572
SapI	GCTCTTC	2 cut(s)	33, 1182
SaqAI	TTAA	6 cut(s)	288, 1152, 1809, 2103, 2325, 2400
Sau96I	GGNCC	1 cut(s)	611
SchI	GAGTC	5 cut(s)	379, 536, 647, 841, 1860
ScrFI	CCNGG	6 cut(s)	766, 1037, 1628, 1691, 2149, 2150
SduI	GDGCHC	4 cut(s)	1370, 1572, 1628, 2409
SexAI	ACCWGGT	2 cut(s)	764, 1035
SfaNI	GCATC	8 cut(s)	468, 731, 772, 994, 1117, 1255, 1507, 1603
SfcI	CTRYAG	1 cut(s)	1509
Sfr274I	CTCGAG	2 cut(s)	1215, 1361
SinI	GGWCC	1 cut(s)	611
SlaI	CTCGAG	2 cut(s)	1215, 1361
SmaI	CCCGGG	1 cut(s)	2150
SmiMI	CAYNNNNRTG	1 cut(s)	1714
SmlI	CTYRAG	7 cut(s)	439, 1215, 1361, 1579, 2102, 2324, 2399
SmoI	CTYRAG	7 cut(s)	439, 1215, 1361, 1579, 2102, 2324, 2399
SsiI	CCGC	9 cut(s)	313, 366, 600, 1332, 1706, 1726, 1754, 2160, 2190
SspMI	CTAG	3 cut(s)	795, 930, 1616
SstI	GAGCTC	1 cut(s)	1572
StyD4I	CCNGG	6 cut(s)	764, 1035, 1626, 1689, 2147, 2148
TaiI	ACGT	7 cut(s)	844, 1089, 1162, 1339, 1863, 1943, 2334
TatI	WGTACW	3 cut(s)	491, 1653, 2177
TauI	GCSGC	3 cut(s)	368, 1757, 2162
TfiI	GAWTC	6 cut(s)	347, 392, 623, 2017, 2221, 2429
Tru1I	TTAA	6 cut(s)	288, 1152, 1809, 2103, 2325, 2400
Tru9I	TTAA	6 cut(s)	288, 1152, 1809, 2103, 2325, 2400
TscAI	CASTG	1 cut(s)	1978
TseFI	GTSAC	1 cut(s)	382
TseI	GCWGC	7 cut(s)	157, 362, 822, 1387, 1424, 1751, 2354
Tsp45I	GTSAC	1 cut(s)	382
TspDTI	ATGAA	6 cut(s)	615, 774, 1224, 1422, 2299, 2351
TspGWI	ACGGA	4 cut(s)	564, 1488, 2376, 2441
TspMI	CCCGGG	1 cut(s)	2148
TspRI	CASTG	1 cut(s)	1978
Vha464I	CTTAAG	3 cut(s)	2102, 2324, 2399
VpaK11BI	GGWCC	1 cut(s)	611
XapI	RAATTY	5 cut(s)	125, 589, 919, 1243, 1904
XceI	RCATGY	2 cut(s)	775, 1000
XhoI	CTCGAG	2 cut(s)	1215, 1361
XmaI	CCCGGG	1 cut(s)	2148
XmiI	GTMKAC	1 cut(s)	2498
XmnI	GAANNNNTTC	1 cut(s)	589
XspI	CTAG	3 cut(s)	795, 930, 1616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.