Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004961

Physical Location & Seq

Genomic Coordinates Reverse (-)

61850085 .. 61855696

Gene Structure 5612 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ59767

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGGATCGAAGTGAAATTCTTGAAGCAGAGAACAATCTCACATTGCAGACACATTCCCCTCTGTCGATTGTTGCCATTGCTATCAATGGCAACAGAAAAAGCAAATACATTGTGAAGTGGGCGCTGGAGAAGTTTATTCCTGAAGGAAACCTCTTTTTCAAGTTGATACATGTCCGAGCAAGAATAACTGGAATTCCAACACCAATGGGGAATCTGATTCCTATTTCAGAAGTACGAGAAGATGTAGTAGCTGCATATAGAAAGGACATGGAGTGGCAGACAAGTGAGCTGCTTCTTCCATACAAGAAAATGTGTACTCTTAAGAAGGTGCAAGTTGATGTTGTAGTCATTGAGTCAGATAATGTGGCAAATGCAATAGCAGAGGAGGTTTCTAAGTCTGCTATAAGCAATCTTGTCATAGGTGCCCCATCTCGTGGCTTGTTTACAAGGAAACAAAAGGGACTGTCCCCAAAAATCTCAGCTTGCACACCAGGATTTTGTACAGTCTATGCTGTTTCAAAAGGAAAATTGTCATCACTACGCCCATCTGATTCCCAGTCTGTTGGAAGCATCAGAGATGATAACAGTGACACTTCTTCTATCAGCAGTTCTTCGAGTAATGCGTCCAGCTCACAAACAGGCACAGATCGTGGATCTGTTGGTTCATACTCTCATTTTCGTTCTCCTTCCCTACCGATGCAGAGATTTCAAGCTCTTTCAAGTATGAACCAAAATCTCCTGAATACAAAAGCAAAGTCAAATGAAACCATTCATTCTAAACACCAGTCTCTGGATCTTGGGGAGGAGAAGGATGCTATGACTATGAACTCTTATCCTAGTAACTCAGATGCTGGACGTGCATTGAGCCACTCCTCTAGTACTTTAAGCTTTGTAACAGATAACCAGTCATGGACTTCTGACCAAGCATCCACCTCAGATGTAGTTAAAAATTATTCATCTGAGAGCCAGGTAAATATCAACCTGGAGCTAGAAAAACTGAGAATTGAACTCAGACATGTCAAAGGAATGTATGCGATGGCTCAAAGTGAGACTATTGATGCTTCTCGGAAGATAAGCAATTTAAGTAATCGGCGGTTGGAGGAAGCAGTAAGGCTCAAGGAGATAAGTGCTATGGAGGAGAAAGCCAAAATGCTGGCAGAACAAGAGAAGGAGAACTATGAAGCTGCTAAGATAGAAGCTGACTACATGAGACAGTGTATTGAAAGTGAAGCTTCTCAAAGGAGAGAAGCAGAAATGAAAGCCTTTCATGATGCCAAGGAAAAAGAAAAGCTTGAAAATGTACTAGTTGGTCCTGTGCAGCAATACCAGAAGTTCAGGTGGGAAGATATTGTTTCTGCAACTTCATCTTTCTCTGAGGATCTTAGGATTGGTATGGGTGCATATGGAACTGTTTATAAGAGCAACTTCCATCATACAACTGCAGCAGTAAAAGTCCTTCACTCTAAAGAGAATCACCAAACTAAGCAATTCTTGCAGGAGCTTGAAATCTTGAGCAAAATCCGACATCCCCATTTGCTTCTCCTCCTTGGTGCATGCCCTGATCATAGTTGCCTAGTGTATGAATACATGGAAAATGGTAGCTTGGATGACAGGCTGAACCAGAAAAATAACACACCCCCCATTCCTTGGTATGAGAGGTTTCGGATTGCTTGGGAAGTAGCCTCAGCTCTAGTCTTTCTTCACAGCTCAAAGCCTAAACCAATTATCCATCGTGATCTGAAACCAGCAAACATCTTGCTTGATCATAACCTTGTGAGCAAGATTGGTGATGTCGGCCTTTCTACAATGCTTAATTTAGACCCTTCTATGTCCAGTATATACAATGAGACAGGCCCTGTTGGGACAATGTGTTACATAGACCCCGAGTATCAACGGACTGGGGTAATCTCTCCAAAATCAGATGTTTATGCTTTGGGAATGGTGATTTTGCAGTTGCTGACTGCAAAACCTGCAATAGCTATAACTCATCTGGTGGAAACAGCTATTCATGGTAAAAGTTTGAAAGATGTTTTAGATCCTAAAGCTGGGTCTTGGCCAATGGAAGAGGCAAGGCAATTAGCTGAATTGGGATTGAGCTGTGCAGAGCTTCGACGTAGGGACAGACCTGACTTGAAAGAGCAAGTGGTTCCAGCACTGGAGAGATTGAAAAAGGTTGCCGATAAAGCAAGAGACTCAGCTTCCACAGTTCAGTGCCTACCTCCTAACCACTTCATCTGTCCAATTCTCAAGGATGTGATGAATGAGCCTTGTGTTGCCGCCGATGGTTACACTTATGATCGCAGGGCAATAGAGAAATGGCTTGAAGAGAATGATAACTCCCCAATGACAAACTTGGCTATGCCAAACAAGCACCTAATACCAAACTATACTCTTCTTTCTGCAATTGTGGAGTGGAAGTCTAGAAAACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

810

Amino Acids

90.54

Weight (kDa)

7.08

Isoelectric Point (pI)

44.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	461 - 710	1e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	462 - 713	7.2e-39	Protein tyrosine and serine/threonine kinase
U-box	PF04564	741 - 809	4.3e-17	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25160 AT5G61550 AT5G61550 AT5G61550 AT5G61550
fragaria_vesca	FvH4_7g27030
malus_domestica	MD01G1177900.v1.1 MD07G1249700.v1.1
prunus_persica	Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1
pyrus_communis	pycom01g18870 pycom07g22310
rosa_chinensis	RchiOBHm_Chr1g0373831
rosa_laevigata	RLG00000026802
rosa_multiflora	Rmu_sc0013735.1_g000010 Rmu_ssc0000206.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283970
rosa_rugosa	Rorug01G0379500
rosa_samantha	Rh1AG389100 Rh1BG353300 Rh1CG366600 Rh1DG384300
rosa_wichuraiana	Rw1G034610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1416
Acc36I	ACCTGC	1 cut(s)	1978
AccB1I	GGYRCC	1 cut(s)	422
AccB7I	CCANNNNNTGG	3 cut(s)	434, 790, 1647
AciI	CCGC	2 cut(s)	1093, 2277
AclWI	GGATC	5 cut(s)	12, 661, 801, 1386, 2030
AcoI	YGGCCR	1 cut(s)	2054
AcsI	RAATTY	2 cut(s)	15, 192
AcuI	CTGAAG	1 cut(s)	162
AfaI	GTAC	5 cut(s)	234, 316, 502, 880, 1302
AfiI	CCNNNNNNNGG	4 cut(s)	434, 790, 1647, 2045
AflII	CTTAAG	1 cut(s)	320
AflIII	ACRYGT	2 cut(s)	169, 1015
AhlI	ACTAGT	1 cut(s)	1303
AjiI	CACGTC	1 cut(s)	857
AjnI	CCWGG	3 cut(s)	490, 966, 981
Alw26I	GTCTC	5 cut(s)	792, 1043, 1204, 1841, 2184
AlwI	GGATC	5 cut(s)	12, 661, 801, 1386, 2030
AlwNI	CAGNNNCTG	4 cut(s)	790, 851, 1856, 1957
Ama87I	CYCGRG	1 cut(s)	1883
AoxI	GGCC	3 cut(s)	1795, 1852, 2054
ApeKI	GCWGC	5 cut(s)	251, 289, 1184, 1318, 1442
ApoI	RAATTY	2 cut(s)	15, 192
ArsI	GACNNNNNNTTYG	2 cut(s)	740, 772
Asp700I	GAANNNNTTC	1 cut(s)	768
AspLEI	GCGC	1 cut(s)	124
AspS9I	GGNCC	2 cut(s)	1310, 1853
AsuHPI	GGTGA	3 cut(s)	1466, 1799, 1954
AvaI	CYCGRG	1 cut(s)	1883
AvaII	GGWCC	1 cut(s)	1310
BaeGI	GKGCMC	1 cut(s)	427
BalI	TGGCCA	1 cut(s)	2056
BanI	GGYRCC	1 cut(s)	422
BarI	GAAGNNNNNNTAC	2 cut(s)	1440, 1472
BauI	CACGAG	1 cut(s)	432
BbvCI	CCTCAGC	1 cut(s)	1684
BbvI	GCAGC	5 cut(s)	238, 276, 1171, 1330, 1454
BccI	CCATC	6 cut(s)	436, 553, 1030, 1437, 1737, 2276
BciT130I	CCWGG	3 cut(s)	492, 968, 983
BclI	TGATCA	2 cut(s)	1561, 1762
BcoDI	GTCTC	5 cut(s)	792, 1043, 1204, 1841, 2184
BcuI	ACTAGT	1 cut(s)	1303
BfaI	CTAG	7 cut(s)	837, 876, 989, 1304, 1574, 1691, 2421
BfmI	CTRYAG	1 cut(s)	1440
BfoI	RGCGCY	1 cut(s)	125
BfrI	CTTAAG	1 cut(s)	320
BfuAI	ACCTGC	1 cut(s)	1978
BisI	GCNGC	6 cut(s)	252, 290, 1185, 1319, 1443, 2277
BlsI	GCNGC	6 cut(s)	253, 291, 1186, 1320, 1444, 2278
BmcAI	AGTACT	1 cut(s)	880
Bme1390I	CCNGG	3 cut(s)	492, 968, 983
Bme18I	GGWCC	1 cut(s)	1310
BmeT110I	CYCGRG	1 cut(s)	1883
BmgBI	CACGTC	1 cut(s)	857
BmgT120I	GGNCC	2 cut(s)	1310, 1853
BmiI	GGNNCC	2 cut(s)	424, 2148
BmrFI	CCNGG	3 cut(s)	492, 968, 983
BmrI	ACTGGG	2 cut(s)	550, 1908
BmsI	GCATC	7 cut(s)	579, 687, 802, 838, 935, 1048, 1261
BmuI	ACTGGG	2 cut(s)	550, 1908
BpmI	CTGGAG	3 cut(s)	146, 1004, 2177
Bpu10I	CCTNAGC	1 cut(s)	1684
BpuEI	CTTGAG	3 cut(s)	1100, 1531, 2231
BsaJI	CCNNGG	3 cut(s)	1275, 1546, 1646
BsaXI	ACNNNNNCTCC	2 cut(s)	2402, 2432
Bsc4I	CCNNNNNNNGG	4 cut(s)	434, 790, 1647, 2045
Bse1I	ACTGG	7 cut(s)	193, 556, 784, 904, 1833, 1903, 2160
Bse3DI	GCAATG	2 cut(s)	41, 75
BseBI	CCWGG	3 cut(s)	492, 968, 983
BseDI	CCNNGG	3 cut(s)	1275, 1546, 1646
BseGI	GGATG	5 cut(s)	817, 926, 1525, 1612, 2257
BseLI	CCNNNNNNNGG	4 cut(s)	434, 790, 1647, 2045
BseMI	GCAATG	2 cut(s)	41, 75
BseMII	CTCAG	9 cut(s)	492, 858, 948, 951, 989, 1024, 1365, 1698, 2208
BseNI	ACTGG	7 cut(s)	193, 556, 784, 904, 1833, 1903, 2160
BseRI	GAGGAG	5 cut(s)	398, 818, 862, 1151, 1532
BseSI	GKGCMC	1 cut(s)	427
BseXI	GCAGC	5 cut(s)	238, 276, 1171, 1330, 1454
BseYI	CCCAGC	1 cut(s)	2045
BsgI	GTGCAG	2 cut(s)	1337, 2121
BshFI	GGCC	3 cut(s)	1797, 1854, 2056
BshNI	GGYRCC	1 cut(s)	422
BsiHKCI	CYCGRG	1 cut(s)	1883
BslFI	GGGAC	4 cut(s)	451, 474, 1876, 2132
BslI	CCNNNNNNNGG	4 cut(s)	434, 790, 1647, 2045
BsmAI	GTCTC	5 cut(s)	792, 1043, 1204, 1841, 2184
BsmFI	GGGAC	4 cut(s)	451, 474, 1876, 2132
BsnI	GGCC	3 cut(s)	1797, 1854, 2056
BsoBI	CYCGRG	1 cut(s)	1883
Bsp1286I	GDGCHC	1 cut(s)	427
Bsp1407I	TGTACA	1 cut(s)	500
BspACI	CCGC	2 cut(s)	1093, 2277
BspANI	GGCC	3 cut(s)	1797, 1854, 2056
BspCNI	CTCAG	9 cut(s)	491, 857, 947, 952, 990, 1023, 1366, 1697, 2207
BspHI	TCATGA	1 cut(s)	1267
BspLI	GGNNCC	2 cut(s)	424, 2148
BspMAI	CTGCAG	1 cut(s)	1444
BspMI	ACCTGC	1 cut(s)	1978
BspPI	GGATC	5 cut(s)	12, 661, 801, 1386, 2030
BspT107I	GGYRCC	1 cut(s)	422
BspTI	CTTAAG	1 cut(s)	320
BsrDI	GCAATG	2 cut(s)	41, 75
BsrGI	TGTACA	1 cut(s)	500
BsrI	ACTGG	7 cut(s)	193, 556, 784, 904, 1833, 1903, 2160
BssECI	CCNNGG	3 cut(s)	1275, 1546, 1646
BssSI	CACGAG	1 cut(s)	432
BssT1I	CCWWGG	3 cut(s)	1275, 1546, 1646
Bst2BI	CACGAG	1 cut(s)	432
Bst2UI	CCWGG	3 cut(s)	492, 968, 983
Bst4CI	ACNGT	7 cut(s)	465, 505, 587, 1215, 1411, 2206, 2430
Bst6I	CTCTTC	3 cut(s)	2058, 2319, 2397
BstAFI	CTTAAG	1 cut(s)	320
BstAPI	GCANNNNNTGC	2 cut(s)	1492, 1970
BstAUI	TGTACA	1 cut(s)	500
BstC8I	GCNNGC	3 cut(s)	484, 1155, 1555
BstF5I	GGATG	5 cut(s)	817, 926, 1525, 1612, 2257
BstH2I	RGCGCY	1 cut(s)	125
BstHHI	GCGC	1 cut(s)	124
BstMAI	GTCTC	5 cut(s)	792, 1043, 1204, 1841, 2184
BstMWI	GCNNNNNNNGC	5 cut(s)	857, 1492, 1970, 2183, 2368
BstNI	CCWGG	3 cut(s)	492, 968, 983
BstNSI	RCATGY	3 cut(s)	173, 1019, 1557
BstSCI	CCNGG	3 cut(s)	490, 966, 981
BstSFI	CTRYAG	1 cut(s)	1440
BstSLI	GKGCMC	1 cut(s)	427
BstV1I	GCAGC	5 cut(s)	238, 276, 1171, 1330, 1454
BstX2I	RGATCY	4 cut(s)	653, 793, 1378, 2035
BstXI	CCANNNNNNTGG	2 cut(s)	563, 1153
BstYI	RGATCY	4 cut(s)	653, 793, 1378, 2035
BsuRI	GGCC	3 cut(s)	1797, 1854, 2056
BtgZI	GCGATG	1 cut(s)	1049
BtrI	CACGTC	1 cut(s)	857
BtsCI	GGATG	5 cut(s)	817, 926, 1525, 1612, 2257
BtsIMutI	CAGTG	4 cut(s)	592, 1220, 2153, 2216
BveI	ACCTGC	1 cut(s)	1978
Cac8I	GCNNGC	3 cut(s)	484, 1155, 1555
CaiI	CAGNNNCTG	4 cut(s)	790, 851, 1856, 1957
CciI	TCATGA	1 cut(s)	1267
CfoI	GCGC	1 cut(s)	124
Cfr13I	GGNCC	2 cut(s)	1310, 1853
CseI	GACGC	1 cut(s)	612
Csp6I	GTAC	5 cut(s)	233, 315, 501, 879, 1301
CviAII	CATG	9 cut(s)	170, 268, 909, 1016, 1207, 1268, 1554, 1588, 2009
CviQI	GTAC	5 cut(s)	233, 315, 501, 879, 1301
EaeI	YGGCCR	1 cut(s)	2054
Eam1104I	CTCTTC	3 cut(s)	2058, 2319, 2397
EarI	CTCTTC	3 cut(s)	2058, 2319, 2397
Eco130I	CCWWGG	3 cut(s)	1275, 1546, 1646
Eco47I	GGWCC	1 cut(s)	1310
Eco57I	CTGAAG	1 cut(s)	162
Eco88I	CYCGRG	1 cut(s)	1883
EcoO109I	RGGNCCY	1 cut(s)	1853
EcoRI	GAATTC	1 cut(s)	192
EcoRII	CCWGG	3 cut(s)	490, 966, 981
EcoT14I	CCWWGG	3 cut(s)	1275, 1546, 1646
ErhI	CCWWGG	3 cut(s)	1275, 1546, 1646
FaeI	CATG	9 cut(s)	173, 271, 912, 1019, 1210, 1271, 1557, 1591, 2012
FalI	AAGNNNNNCTT	8 cut(s)	1216, 1248, 1275, 1307, 1409, 1441, 1475, 1507
FaqI	GGGAC	4 cut(s)	451, 474, 1876, 2132
FatI	CATG	9 cut(s)	169, 267, 908, 1015, 1206, 1267, 1553, 1587, 2008
FauNDI	CATATG	1 cut(s)	1402
FbaI	TGATCA	2 cut(s)	1561, 1762
Fnu4HI	GCNGC	6 cut(s)	252, 290, 1185, 1319, 1443, 2277
FokI	GGATG	5 cut(s)	824, 913, 1512, 1619, 2264
Fsp4HI	GCNGC	6 cut(s)	252, 290, 1185, 1319, 1443, 2277
FspBI	CTAG	7 cut(s)	837, 876, 989, 1304, 1574, 1691, 2421
GlaI	GCGC	1 cut(s)	123
GluI	GCNGC	6 cut(s)	252, 290, 1185, 1319, 1443, 2277
GsaI	CCCAGC	1 cut(s)	2049
GsuI	CTGGAG	3 cut(s)	146, 1004, 2177
HaeII	RGCGCY	1 cut(s)	125
HaeIII	GGCC	3 cut(s)	1797, 1854, 2056
HgaI	GACGC	1 cut(s)	612
HhaI	GCGC	1 cut(s)	124
Hin1II	CATG	9 cut(s)	173, 271, 912, 1019, 1210, 1271, 1557, 1591, 2012
Hin6I	GCGC	1 cut(s)	122
HinP1I	GCGC	1 cut(s)	122
HindIII	AAGCTT	3 cut(s)	886, 1230, 1289
HinfI	GANTC	6 cut(s)	211, 217, 353, 551, 1471, 2192
HphI	GGTGA	3 cut(s)	1466, 1799, 1954
Hpy166II	GTNNAC	2 cut(s)	315, 444
Hpy188III	TCNNGA	8 cut(s)	20, 140, 739, 791, 1268, 1510, 1733, 2421
Hpy8I	GTNNAC	2 cut(s)	315, 444
Hpy99I	CGWCG	1 cut(s)	2115
HpyAV	CCTTC	7 cut(s)	137, 319, 696, 802, 1162, 1466, 1833
HpyCH4III	ACNGT	7 cut(s)	465, 505, 587, 1215, 1411, 2206, 2430
HpyCH4IV	ACGT	2 cut(s)	856, 2113
HpyF10VI	GCNNNNNNNGC	5 cut(s)	857, 1492, 1970, 2183, 2368
HpySE526I	ACGT	2 cut(s)	856, 2113
Hsp92II	CATG	9 cut(s)	173, 271, 912, 1019, 1210, 1271, 1557, 1591, 2012
HspAI	GCGC	1 cut(s)	122
Ksp22I	TGATCA	2 cut(s)	1561, 1762
LmnI	GCTCC	2 cut(s)	985, 1498
Lsp1109I	GCAGC	5 cut(s)	238, 276, 1171, 1330, 1454
LweI	GCATC	7 cut(s)	579, 687, 802, 838, 935, 1048, 1261
MaeI	CTAG	7 cut(s)	837, 876, 989, 1304, 1574, 1691, 2421
MaeII	ACGT	2 cut(s)	856, 2113
MaeIII	GTNAC	5 cut(s)	587, 839, 892, 1871, 2285
MfeI	CAATTG	1 cut(s)	2403
MflI	RGATCY	4 cut(s)	653, 793, 1378, 2035
MhlI	GDGCHC	1 cut(s)	427
MlsI	TGGCCA	1 cut(s)	2056
MluNI	TGGCCA	1 cut(s)	2056
MlyI	GAGTC	2 cut(s)	362, 2186
MmeI	TCCRAC	4 cut(s)	221, 544, 1077, 1546
Mox20I	TGGCCA	1 cut(s)	2056
MroXI	GAANNNNTTC	1 cut(s)	768
MscI	TGGCCA	1 cut(s)	2056
MseI	TTAA	5 cut(s)	321, 884, 945, 1082, 1812
Msp20I	TGGCCA	1 cut(s)	2056
MspCI	CTTAAG	1 cut(s)	320
MspR9I	CCNGG	3 cut(s)	492, 968, 983
MunI	CAATTG	1 cut(s)	2403
MvaI	CCWGG	3 cut(s)	492, 968, 983
MwoI	GCNNNNNNNGC	5 cut(s)	857, 1492, 1970, 2183, 2368
NdeI	CATATG	1 cut(s)	1402
NlaIII	CATG	9 cut(s)	173, 271, 912, 1019, 1210, 1271, 1557, 1591, 2012
NlaIV	GGNNCC	2 cut(s)	424, 2148
NmuCI	GTSAC	1 cut(s)	587
NspI	RCATGY	3 cut(s)	173, 1019, 1557
PaeI	GCATGC	1 cut(s)	1557
PagI	TCATGA	1 cut(s)	1267
PciI	ACATGT	2 cut(s)	169, 1015
PcsI	WCGNNNNNNNCGW	1 cut(s)	620
PdmI	GAANNNNTTC	1 cut(s)	768
PfeI	GAWTC	4 cut(s)	211, 217, 551, 1471
PflMI	CCANNNNNTGG	3 cut(s)	434, 790, 1647
PkrI	GCNGC	6 cut(s)	253, 291, 1186, 1320, 1444, 2278
PleI	GAGTC	2 cut(s)	361, 2186
PpsI	GAGTC	2 cut(s)	361, 2186
PscI	ACATGT	2 cut(s)	169, 1015
PsiI	TTATAA	1 cut(s)	1416
Psp6I	CCWGG	3 cut(s)	490, 966, 981
PspFI	CCCAGC	1 cut(s)	2045
PspGI	CCWGG	3 cut(s)	490, 966, 981
PspN4I	GGNNCC	2 cut(s)	424, 2148
PspPI	GGNCC	2 cut(s)	1310, 1853
PstI	CTGCAG	1 cut(s)	1444
PstNI	CAGNNNCTG	4 cut(s)	790, 851, 1856, 1957
PsuI	RGATCY	4 cut(s)	653, 793, 1378, 2035
RsaI	GTAC	5 cut(s)	234, 316, 502, 880, 1302
RsaNI	GTAC	5 cut(s)	233, 315, 501, 879, 1301
SaqAI	TTAA	5 cut(s)	321, 884, 945, 1082, 1812
SatI	GCNGC	6 cut(s)	252, 290, 1185, 1319, 1443, 2277
Sau96I	GGNCC	2 cut(s)	1310, 1853
ScaI	AGTACT	1 cut(s)	880
SchI	GAGTC	2 cut(s)	362, 2186
ScrFI	CCNGG	3 cut(s)	492, 968, 983
SduI	GDGCHC	1 cut(s)	427
SfaNI	GCATC	7 cut(s)	579, 687, 802, 838, 935, 1048, 1261
SfcI	CTRYAG	1 cut(s)	1440
SinI	GGWCC	1 cut(s)	1310
SmlI	CTYRAG	4 cut(s)	320, 1115, 1510, 2246
SmoI	CTYRAG	4 cut(s)	320, 1115, 1510, 2246
SpeI	ACTAGT	1 cut(s)	1303
SphI	GCATGC	1 cut(s)	1557
SsiI	CCGC	2 cut(s)	1093, 2277
SspMI	CTAG	7 cut(s)	837, 876, 989, 1304, 1574, 1691, 2421
StyD4I	CCNGG	3 cut(s)	490, 966, 981
StyI	CCWWGG	3 cut(s)	1275, 1546, 1646
TaaI	ACNGT	7 cut(s)	465, 505, 587, 1215, 1411, 2206, 2430
TaiI	ACGT	2 cut(s)	859, 2116
TaqI	TCGA	4 cut(s)	7, 65, 614, 2110
TatI	WGTACW	4 cut(s)	314, 500, 878, 1300
TauI	GCSGC	1 cut(s)	2279
TfiI	GAWTC	4 cut(s)	211, 217, 551, 1471
Tru1I	TTAA	5 cut(s)	321, 884, 945, 1082, 1812
Tru9I	TTAA	5 cut(s)	321, 884, 945, 1082, 1812
TscAI	CASTG	4 cut(s)	592, 1220, 2160, 2216
TseFI	GTSAC	1 cut(s)	587
TseI	GCWGC	5 cut(s)	251, 289, 1184, 1318, 1442
Tsp45I	GTSAC	1 cut(s)	587
TspGWI	ACGGA	1 cut(s)	1909
TspRI	CASTG	4 cut(s)	592, 1220, 2160, 2216
Van91I	CCANNNNNTGG	3 cut(s)	434, 790, 1647
Vha464I	CTTAAG	1 cut(s)	320
VpaK11BI	GGWCC	1 cut(s)	1310
XapI	RAATTY	2 cut(s)	15, 192
XbaI	TCTAGA	1 cut(s)	2420
XceI	RCATGY	3 cut(s)	173, 1019, 1557
XmnI	GAANNNNTTC	1 cut(s)	768
XspI	CTAG	7 cut(s)	837, 876, 989, 1304, 1574, 1691, 2421
ZrmI	AGTACT	1 cut(s)	880

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.