Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004961

Physical Location & Seq

Genomic Coordinates Reverse (-)

27832626 .. 27836151

Gene Structure 3526 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1177900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGGATGGGAGTGAAATTACTGAAGGAGGTGAAAATATTCAACCTTCTTCTCTTTCAATTGTTGGCATTGCTGTCAGTGGGGACAGAAATAGCAAATACATAGTTAGGTGGGCTCTGGATAAGTTTGTTCCGGAGGGAAATGTTTTCTTCAAGTTGATTCATGTCCGGGAAAGGATCACCGGAGTTCCAACACCAATGGGAAGTCTGATTCCTATTTCGCGAGTACGAGAGGACGTAGTAGCTGCCTATCGAAAGGAAATGGAGTGGCAGGCGAGTGAAATGCTTGTTCCATACAAGAAAATGTGTGCTTATAGGAAGGTGCAAGTAGAAGTTGCACTTGCTGAATCAGATGACGTTGCAAATGCAATAGCAGAAGAGGTTGCTGAGTCTGCTATAAGCAATCTCGTCTTAGGTGCCCCATCTCGTGGCATGTTTAAAAGGAAACAAAAGGGACTCTCCTTAAAAATCTCAGCCTGCACTCCAAGATCTTGCACGGTGTATGCTGTTTCAAAAGGAAAGTTGTCATCCCTACGTGCATCTGATCCCGCTGTTTCAAAAGGAAAGCTGTCATCCCTACGCACATCTGATTCTGAGTCAGTAGGAAGCATCAAAGATGACAACAGTGACACATGTTCTATCAGCAGTTCTTCGAGTTACACGTCTAGCTTACAGACAGGCACAGATGGCAGCTCGGTTGGTTCATACTCTCATTTCCGTTCTCCTCCTCTGACAGTGCAGCGATTTCAATCTCTTACAAATATCAACAAGACCCTTCTTCGTACAAAGACAAATTCAAATGACACCATCCATTCTAGATGCCAATCTCAGGATCTTGGAAGCTTTGTTGCAGATAACCAGTCATGGACTTCAGATCAAGTGTCCACCTCAGACGTGGTTACAGATTATTCATCGGAGAGCCAGGCGACTATCAACCTCGAGATAGAAAAGCTGAGAATTGAACTCAGACATGTCAAAGGAATGTATGCAATGGCTCAAAGTGAGACTATGGATGCTTCTAGGAAGATAAATGATTTAACTAAACGCCGGTTTGAAGAAGCATTAAGGCTCAAGGAGATAAACTCCATGGAGGAGAAAGCGAAAGTATTTGCAACAAAAGAAAAGGAGAAGTATGAATCTGCTATGAGGGAAGCTGATTACATGAAAGAGTGTGTCGAAAGAGAAGTTTCACTAAGGAGAGAAGCAGAGATGAAAGCCATGCATGGTGCCAAGGAGAACGAAAAACTTGAAAATGCACTTGTAGGTCCTGTGTTATACCAGAAGTTCTCGTGGGATGAGATTGTTACAGCCACCTCATCTTTCTCCGAGGATCATAAGATTGGAATGGGAGCATACGGAAATGTCTATAAATGCAGCTTTCATCATACAACTGCAGCAGTGAAAGTCCTTCACTCTATAGAGAATCACCAAACTAAGCAATTCCAGCAGGAGCTTGAGATCTTGAGCAAAATCCGCCATCCCCATTTGCTTCTCCTTCTCGGAGCATGTCCTGATCACAGTAGCCTAGTCTACGAGTACATGGAAAGCGGTAGCTTGGAGGACAGGTTGCTGCAGAAAAACGGTACACCTCCTATTCCTTGGTTTGAGAGGTTCCGAATTGCTTGGGAGATAGCCTCAACTCTCGTCTTTCTTCACAGCTCAAAGCCAAAACCAATCATCCATCGTGATCTGAAACCAGCCAATATCTTGCTAGATCATAAACTTGTGAGCAAGATTGGCGACGTTGGCCTTGCCACAATGCTTAATTCAGATCCTTCTACGTCCAGTATATACAATGACACAGGGCCTGTTGGCACTCTCTCTTACATAGACCCCGAGTACCAAAGGACAGGAATAATCTCTCCACAATCGGATGTTTATGCTTTCGGAATGGTGATCTTGCAGTTGCTTACTGCAAAACCAGCAAGAGCTATAACACATCTGGTAGAAACAGCTGTTAACGATAAAAATTTAATGGATATTTTGGACCCTAAAGCCGGTCTTTGGCCAATGGAAGAGACAAGGCAGTTGGCTGAATTGGGATTGAGCTGCGCAGAGCTTCGACGCATAGACAGACCTGACTTGAAAGAACAAGTAGTTCCTTTACTTGAGAGATTGAAGGAGGTTGCTTATGCAGCGAAAAATTCAGCTTCTGCGGTTCATTGCCTACCTCCTAACCACTTCATCTGTCGAATTCTTAAGGATGTGATGAATGAGCCTTGTGTGGCGGCAGATGGTTACACTTACGACCGTAAAGCGATCGAGACATGGCTCGAAGAGAACGATAACTCGCCGGTGACAAATGTGCCATTGCCAAACAAGAATGTAATGCCAAATTATACTCTTCTTTCTGCAATTATGGAGTGGAAGTCCAGAGAACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

794

Amino Acids

88.49

Weight (kDa)

6.3

Isoelectric Point (pI)

46.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	441 - 654	1.4e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	443 - 696	7.3e-38	Protein tyrosine and serine/threonine kinase
U-box	PF04564	724 - 791	3.1e-16	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25160 AT5G61550 AT5G61550 AT5G61550 AT5G61550
fragaria_vesca	FvH4_7g27030
malus_domestica	MD01G1177900.v1.1 MD07G1249700.v1.1
prunus_persica	Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1 Prupe.2G273400_v2.0.a1
pyrus_communis	pycom01g18870 pycom07g22310
rosa_chinensis	RchiOBHm_Chr1g0373831
rosa_laevigata	RLG00000026802
rosa_multiflora	Rmu_sc0013735.1_g000010 Rmu_ssc0000206.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283970
rosa_rugosa	Rorug01G0379500
rosa_samantha	Rh1AG389100 Rh1BG353300 Rh1CG366600 Rh1DG384300
rosa_wichuraiana	Rw1G034610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2050
AccB1I	GGYRCC	2 cut(s)	413, 1223
AccB7I	CCANNNNNTGG	1 cut(s)	425
AccI	GTMKAC	1 cut(s)	1527
AccII	CGCG	1 cut(s)	220
AccIII	TCCGGA	1 cut(s)	130
AciI	CCGC	5 cut(s)	546, 1471, 1545, 2153, 2225
AclWI	GGATC	5 cut(s)	182, 536, 837, 1335, 1763
AcoI	YGGCCR	1 cut(s)	2003
AcsI	RAATTY	4 cut(s)	790, 1966, 2140, 2190
AcuI	CTGAAG	2 cut(s)	42, 852
AfaI	GTAC	5 cut(s)	225, 781, 1535, 1582, 1838
AfiI	CCNNNNNNNGG	4 cut(s)	425, 826, 1994, 2001
AflII	CTTAAG	1 cut(s)	2195
AflIII	ACRYGT	3 cut(s)	629, 657, 967
AjiI	CACGTC	2 cut(s)	660, 892
AjnI	CCWGG	1 cut(s)	918
Alw26I	GTCTC	3 cut(s)	995, 2009, 2255
AlwI	GGATC	5 cut(s)	182, 536, 837, 1335, 1763
AlwNI	CAGNNNCTG	2 cut(s)	1805, 2150
Ama87I	CYCGRG	2 cut(s)	935, 1832
Aor13HI	TCCGGA	1 cut(s)	130
AoxI	GGCC	3 cut(s)	1744, 1802, 2003
ApeKI	GCWGC	8 cut(s)	242, 687, 736, 1371, 1391, 1567, 2046, 2132
ApoI	RAATTY	4 cut(s)	790, 1966, 2140, 2190
Asp700I	GAANNNNTTC	1 cut(s)	36
AspLEI	GCGC	1 cut(s)	2051
AspS9I	GGNCC	3 cut(s)	1262, 1802, 1984
AsuC2I	CCSGG	1 cut(s)	167
AsuHPI	GGTGA	5 cut(s)	41, 169, 1415, 1903, 2305
AvaI	CYCGRG	2 cut(s)	935, 1832
AvaII	GGWCC	2 cut(s)	1262, 1984
BaeGI	GKGCMC	1 cut(s)	418
BalI	TGGCCA	1 cut(s)	2005
BanI	GGYRCC	2 cut(s)	413, 1223
BanII	GRGCYC	1 cut(s)	115
BauI	CACGAG	2 cut(s)	423, 1285
BbvI	GCAGC	8 cut(s)	229, 699, 748, 1383, 1403, 1554, 2033, 2144
BccI	CCATC	6 cut(s)	427, 677, 812, 1482, 1686, 2225
BcgI	CGANNNNNNTGC	2 cut(s)	262, 296
BciT130I	CCWGG	1 cut(s)	920
BclI	TGATCA	1 cut(s)	1510
BcnI	CCSGG	1 cut(s)	167
BcoDI	GTCTC	3 cut(s)	995, 2009, 2255
BfaI	CTAG	5 cut(s)	663, 813, 1017, 1523, 1709
BfmI	CTRYAG	3 cut(s)	1389, 1413, 1568
BfrI	CTTAAG	1 cut(s)	2195
BglII	AGATCT	2 cut(s)	485, 1455
BisI	GCNGC	9 cut(s)	243, 688, 737, 1372, 1392, 1568, 2047, 2133, 2226
BlsI	GCNGC	9 cut(s)	244, 689, 738, 1373, 1393, 1569, 2048, 2134, 2227
Bme1390I	CCNGG	2 cut(s)	167, 920
Bme18I	GGWCC	2 cut(s)	1262, 1984
BmeT110I	CYCGRG	2 cut(s)	935, 1832
BmgBI	CACGTC	2 cut(s)	660, 892
BmgT120I	GGNCC	3 cut(s)	1262, 1802, 1984
BmiI	GGNNCC	4 cut(s)	415, 1225, 1610, 1986
BmrFI	CCNGG	2 cut(s)	167, 920
BmsI	GCATC	4 cut(s)	545, 615, 806, 1000
BplI	GAGNNNNNCTC	4 cut(s)	1064, 1096, 1616, 1648
BpuEI	CTTGAG	4 cut(s)	1052, 1472, 1480, 2126
BpuMI	CCSGG	1 cut(s)	167
BsaAI	YACGTR	1 cut(s)	533
BsaBI	GATNNNNATC	2 cut(s)	745, 820
BsaJI	CCNNGG	4 cut(s)	1083, 1227, 1323, 1595
BsaWI	WCCGGW	2 cut(s)	130, 179
BsaXI	ACNNNNNCTCC	4 cut(s)	1547, 1577, 2351, 2381
Bsc4I	CCNNNNNNNGG	4 cut(s)	425, 826, 1994, 2001
Bse118I	RCCGGY	3 cut(s)	1044, 1994, 2290
Bse1I	ACTGG	2 cut(s)	856, 1782
Bse3DI	GCAATG	4 cut(s)	66, 993, 2158, 2306
Bse8I	GATNNNNATC	2 cut(s)	745, 820
BseAI	TCCGGA	1 cut(s)	130
BseBI	CCWGG	1 cut(s)	920
BseDI	CCNNGG	4 cut(s)	1083, 1227, 1323, 1595
BseJI	GATNNNNATC	2 cut(s)	745, 820
BseLI	CCNNNNNNNGG	4 cut(s)	425, 826, 1994, 2001
BseMI	GCAATG	4 cut(s)	66, 993, 2158, 2306
BseMII	CTCAG	7 cut(s)	375, 483, 582, 839, 900, 941, 976
BseNI	ACTGG	2 cut(s)	856, 1782
BseRI	GAGGAG	3 cut(s)	711, 714, 1103
BseSI	GKGCMC	1 cut(s)	418
BseXI	GCAGC	8 cut(s)	229, 699, 748, 1383, 1403, 1554, 2033, 2144
BsgI	GTGCAG	2 cut(s)	460, 755
Bsh1236I	CGCG	1 cut(s)	220
Bsh1285I	CGRYCG	2 cut(s)	2248, 2259
BshFI	GGCC	3 cut(s)	1746, 1804, 2005
BshNI	GGYRCC	2 cut(s)	413, 1223
BsiEI	CGRYCG	2 cut(s)	2248, 2259
BsiHKCI	CYCGRG	2 cut(s)	935, 1832
BsiSI	CCGG	6 cut(s)	131, 166, 180, 1045, 1995, 2291
BslFI	GGGAC	2 cut(s)	95, 465
BslI	CCNNNNNNNGG	4 cut(s)	425, 826, 1994, 2001
BsmAI	GTCTC	3 cut(s)	995, 2009, 2255
BsmFI	GGGAC	2 cut(s)	95, 465
BsnI	GGCC	3 cut(s)	1746, 1804, 2005
BsoBI	CYCGRG	2 cut(s)	935, 1832
Bsp1286I	GDGCHC	2 cut(s)	115, 418
Bsp13I	TCCGGA	1 cut(s)	130
Bsp19I	CCATGG	1 cut(s)	1083
Bsp68I	TCGCGA	1 cut(s)	220
BspACI	CCGC	5 cut(s)	546, 1471, 1545, 2153, 2225
BspANI	GGCC	3 cut(s)	1746, 1804, 2005
BspCNI	CTCAG	7 cut(s)	376, 482, 583, 838, 899, 942, 975
BspEI	TCCGGA	1 cut(s)	130
BspFNI	CGCG	1 cut(s)	220
BspLI	GGNNCC	4 cut(s)	415, 1225, 1610, 1986
BspMAI	CTGCAG	2 cut(s)	1393, 1572
BspPI	GGATC	5 cut(s)	182, 536, 837, 1335, 1763
BspT107I	GGYRCC	2 cut(s)	413, 1223
BspTI	CTTAAG	1 cut(s)	2195
BsrDI	GCAATG	4 cut(s)	66, 993, 2158, 2306
BsrFI	RCCGGY	3 cut(s)	1044, 1994, 2290
BsrI	ACTGG	2 cut(s)	856, 1782
BssAI	RCCGGY	3 cut(s)	1044, 1994, 2290
BssECI	CCNNGG	4 cut(s)	1083, 1227, 1323, 1595
BssSI	CACGAG	2 cut(s)	423, 1285
BssT1I	CCWWGG	3 cut(s)	1083, 1227, 1595
Bst2BI	CACGAG	2 cut(s)	423, 1285
Bst2UI	CCWGG	1 cut(s)	920
Bst4CI	ACNGT	6 cut(s)	496, 623, 733, 1517, 1580, 2249
Bst6I	CTCTTC	4 cut(s)	369, 2007, 2268, 2346
BstAFI	CTTAAG	1 cut(s)	2195
BstBAI	YACGTR	1 cut(s)	533
BstC8I	GCNNGC	2 cut(s)	270, 475
BstDSI	CCRYGG	1 cut(s)	1083
BstFNI	CGCG	1 cut(s)	220
BstHHI	GCGC	1 cut(s)	2051
BstMAI	GTCTC	3 cut(s)	995, 2009, 2255
BstMCI	CGRYCG	2 cut(s)	2248, 2259
BstMWI	GCNNNNNNNGC	8 cut(s)	389, 684, 1441, 1470, 1734, 1743, 1919, 2132
BstNI	CCWGG	1 cut(s)	920
BstNSI	RCATGY	4 cut(s)	433, 633, 971, 1506
BstSCI	CCNGG	2 cut(s)	165, 918
BstSFI	CTRYAG	3 cut(s)	1389, 1413, 1568
BstSLI	GKGCMC	1 cut(s)	418
BstUI	CGCG	1 cut(s)	220
BstV1I	GCAGC	8 cut(s)	229, 699, 748, 1383, 1403, 1554, 2033, 2144
BstX2I	RGATCY	4 cut(s)	485, 829, 1455, 1768
BstYI	RGATCY	4 cut(s)	485, 829, 1455, 1768
BsuRI	GGCC	3 cut(s)	1746, 1804, 2005
BtgI	CCRYGG	1 cut(s)	1083
BtrI	CACGTC	2 cut(s)	660, 892
BtsI	GCAGTG	1 cut(s)	1401
BtsIMutI	CAGTG	4 cut(s)	82, 628, 738, 1401
BtuMI	TCGCGA	1 cut(s)	220
Cac8I	GCNNGC	2 cut(s)	270, 475
CaiI	CAGNNNCTG	2 cut(s)	1805, 2150
CfoI	GCGC	1 cut(s)	2051
Cfr10I	RCCGGY	3 cut(s)	1044, 1994, 2290
Cfr13I	GGNCC	3 cut(s)	1262, 1802, 1984
CseI	GACGC	1 cut(s)	2070
Csp6I	GTAC	5 cut(s)	224, 780, 1534, 1581, 1837
CviQI	GTAC	5 cut(s)	224, 780, 1534, 1581, 1837
DraI	TTTAAA	1 cut(s)	436
EaeI	YGGCCR	1 cut(s)	2003
Eam1104I	CTCTTC	4 cut(s)	369, 2007, 2268, 2346
EarI	CTCTTC	4 cut(s)	369, 2007, 2268, 2346
EciI	GGCGGA	1 cut(s)	1460
Eco130I	CCWWGG	3 cut(s)	1083, 1227, 1595
Eco24I	GRGCYC	1 cut(s)	115
Eco47I	GGWCC	2 cut(s)	1262, 1984
Eco57I	CTGAAG	2 cut(s)	42, 852
Eco88I	CYCGRG	2 cut(s)	935, 1832
EcoO109I	RGGNCCY	2 cut(s)	1262, 1802
EcoRI	GAATTC	1 cut(s)	2190
EcoRII	CCWGG	1 cut(s)	918
EcoT14I	CCWWGG	3 cut(s)	1083, 1227, 1595
EcoT22I	ATGCAT	1 cut(s)	1221
EcoT38I	GRGCYC	1 cut(s)	115
ErhI	CCWWGG	3 cut(s)	1083, 1227, 1595
FalI	AAGNNNNNCTT	4 cut(s)	321, 353, 1983, 2015
FaqI	GGGAC	2 cut(s)	95, 465
FauI	CCCGC	1 cut(s)	553
FbaI	TGATCA	1 cut(s)	1510
FblI	GTMKAC	1 cut(s)	1527
Fnu4HI	GCNGC	9 cut(s)	243, 688, 737, 1372, 1392, 1568, 2047, 2133, 2226
FriOI	GRGCYC	1 cut(s)	115
Fsp4HI	GCNGC	9 cut(s)	243, 688, 737, 1372, 1392, 1568, 2047, 2133, 2226
FspBI	CTAG	5 cut(s)	663, 813, 1017, 1523, 1709
FspI	TGCGCA	1 cut(s)	2050
GlaI	GCGC	1 cut(s)	2050
GluI	GCNGC	9 cut(s)	243, 688, 737, 1372, 1392, 1568, 2047, 2133, 2226
HaeIII	GGCC	3 cut(s)	1746, 1804, 2005
HapII	CCGG	6 cut(s)	131, 166, 180, 1045, 1995, 2291
HgaI	GACGC	1 cut(s)	2070
HhaI	GCGC	1 cut(s)	2051
Hin6I	GCGC	1 cut(s)	2049
HinP1I	GCGC	1 cut(s)	2049
HincII	GTYRAC	1 cut(s)	1957
HindII	GTYRAC	1 cut(s)	1957
HindIII	AAGCTT	1 cut(s)	838
HinfI	GANTC	9 cut(s)	157, 208, 344, 386, 453, 587, 593, 1133, 1420
HpaI	GTTAAC	1 cut(s)	1957
HpaII	CCGG	6 cut(s)	131, 166, 180, 1045, 1995, 2291
HphI	GGTGA	5 cut(s)	41, 169, 1415, 1903, 2305
Hpy166II	GTNNAC	4 cut(s)	882, 1528, 1583, 1957
Hpy8I	GTNNAC	4 cut(s)	882, 1528, 1583, 1957
Hpy99I	CGWCG	2 cut(s)	1742, 2064
HpyAV	CCTTC	8 cut(s)	17, 54, 310, 782, 1415, 1502, 1782, 2110
HpyCH4III	ACNGT	6 cut(s)	496, 623, 733, 1517, 1580, 2249
HpyCH4IV	ACGT	7 cut(s)	234, 354, 532, 659, 891, 1740, 1778
HpyF10VI	GCNNNNNNNGC	8 cut(s)	389, 684, 1441, 1470, 1734, 1743, 1919, 2132
HpySE526I	ACGT	7 cut(s)	234, 354, 532, 659, 891, 1740, 1778
HspAI	GCGC	1 cut(s)	2049
Kpn2I	TCCGGA	1 cut(s)	130
Ksp22I	TGATCA	1 cut(s)	1510
KspAI	GTTAAC	1 cut(s)	1957
LmnI	GCTCC	3 cut(s)	1346, 1447, 1499
Lsp1109I	GCAGC	8 cut(s)	229, 699, 748, 1383, 1403, 1554, 2033, 2144
LweI	GCATC	4 cut(s)	545, 615, 806, 1000
MaeI	CTAG	5 cut(s)	663, 813, 1017, 1523, 1709
MaeII	ACGT	7 cut(s)	234, 354, 532, 659, 891, 1740, 1778
MaeIII	GTNAC	6 cut(s)	623, 653, 895, 1300, 2234, 2293
MfeI	CAATTG	1 cut(s)	57
MflI	RGATCY	4 cut(s)	485, 829, 1455, 1768
MhlI	GDGCHC	2 cut(s)	115, 418
MlsI	TGGCCA	1 cut(s)	2005
MluNI	TGGCCA	1 cut(s)	2005
MlyI	GAGTC	3 cut(s)	395, 447, 602
MmeI	TCCRAC	1 cut(s)	212
Mox20I	TGGCCA	1 cut(s)	2005
Mph1103I	ATGCAT	1 cut(s)	1221
MroI	TCCGGA	1 cut(s)	130
MroXI	GAANNNNTTC	1 cut(s)	36
MscI	TGGCCA	1 cut(s)	2005
MseI	TTAA	8 cut(s)	435, 461, 1034, 1061, 1761, 1956, 1970, 2196
Msp20I	TGGCCA	1 cut(s)	2005
MspA1I	CMGCKG	2 cut(s)	548, 1952
MspCI	CTTAAG	1 cut(s)	2195
MspI	CCGG	6 cut(s)	131, 166, 180, 1045, 1995, 2291
MspR9I	CCNGG	2 cut(s)	167, 920
MunI	CAATTG	1 cut(s)	57
MvaI	CCWGG	1 cut(s)	920
MvnI	CGCG	1 cut(s)	220
MwoI	GCNNNNNNNGC	8 cut(s)	389, 684, 1441, 1470, 1734, 1743, 1919, 2132
NciI	CCSGG	1 cut(s)	167
NcoI	CCATGG	1 cut(s)	1083
NlaIV	GGNNCC	4 cut(s)	415, 1225, 1610, 1986
NmuCI	GTSAC	2 cut(s)	623, 2293
NruI	TCGCGA	1 cut(s)	220
NsbI	TGCGCA	1 cut(s)	2050
NsiI	ATGCAT	1 cut(s)	1221
NspI	RCATGY	4 cut(s)	433, 633, 971, 1506
PaeR7I	CTCGAG	1 cut(s)	935
PciI	ACATGT	2 cut(s)	629, 967
PcsI	WCGNNNNNNNCGW	2 cut(s)	656, 2277
PdmI	GAANNNNTTC	1 cut(s)	36
PfeI	GAWTC	6 cut(s)	157, 208, 344, 587, 1133, 1420
PflMI	CCANNNNNTGG	1 cut(s)	425
PfoI	TCCNGGA	1 cut(s)	165
PkrI	GCNGC	9 cut(s)	244, 689, 738, 1373, 1393, 1569, 2048, 2134, 2227
Ple19I	CGATCG	1 cut(s)	2259
PleI	GAGTC	3 cut(s)	394, 447, 601
PpsI	GAGTC	3 cut(s)	394, 447, 601
Ppu21I	YACGTR	1 cut(s)	533
PpuMI	RGGWCCY	1 cut(s)	1262
PscI	ACATGT	2 cut(s)	629, 967
Psp5II	RGGWCCY	1 cut(s)	1262
Psp6I	CCWGG	1 cut(s)	918
PspGI	CCWGG	1 cut(s)	918
PspN4I	GGNNCC	4 cut(s)	415, 1225, 1610, 1986
PspPI	GGNCC	3 cut(s)	1262, 1802, 1984
PspPPI	RGGWCCY	1 cut(s)	1262
PstI	CTGCAG	2 cut(s)	1393, 1572
PstNI	CAGNNNCTG	2 cut(s)	1805, 2150
PsuI	RGATCY	4 cut(s)	485, 829, 1455, 1768
PvuI	CGATCG	1 cut(s)	2259
PvuII	CAGCTG	1 cut(s)	1952
RruI	TCGCGA	1 cut(s)	220
RsaI	GTAC	5 cut(s)	225, 781, 1535, 1582, 1838
RsaNI	GTAC	5 cut(s)	224, 780, 1534, 1581, 1837
SaqAI	TTAA	8 cut(s)	435, 461, 1034, 1061, 1761, 1956, 1970, 2196
SatI	GCNGC	9 cut(s)	243, 688, 737, 1372, 1392, 1568, 2047, 2133, 2226
Sau96I	GGNCC	3 cut(s)	1262, 1802, 1984
SchI	GAGTC	3 cut(s)	395, 447, 602
ScrFI	CCNGG	2 cut(s)	167, 920
SduI	GDGCHC	2 cut(s)	115, 418
SfaNI	GCATC	4 cut(s)	545, 615, 806, 1000
SfcI	CTRYAG	3 cut(s)	1389, 1413, 1568
Sfr274I	CTCGAG	1 cut(s)	935
SgrAI	CRCCGGYG	1 cut(s)	2290
SinI	GGWCC	2 cut(s)	1262, 1984
SlaI	CTCGAG	1 cut(s)	935
SmlI	CTYRAG	6 cut(s)	935, 1067, 1451, 1459, 2105, 2195
SmoI	CTYRAG	6 cut(s)	935, 1067, 1451, 1459, 2105, 2195
SsiI	CCGC	5 cut(s)	546, 1471, 1545, 2153, 2225
SspI	AATATT	1 cut(s)	37
SspMI	CTAG	5 cut(s)	663, 813, 1017, 1523, 1709
StyD4I	CCNGG	2 cut(s)	165, 918
StyI	CCWWGG	3 cut(s)	1083, 1227, 1595
TaaI	ACNGT	6 cut(s)	496, 623, 733, 1517, 1580, 2249
TaiI	ACGT	7 cut(s)	237, 357, 535, 662, 894, 1743, 1781
TaqI	TCGA	8 cut(s)	250, 650, 936, 1173, 2059, 2188, 2259, 2271
TatI	WGTACW	1 cut(s)	1533
TauI	GCSGC	1 cut(s)	2228
TfiI	GAWTC	6 cut(s)	157, 208, 344, 587, 1133, 1420
Tru1I	TTAA	8 cut(s)	435, 461, 1034, 1061, 1761, 1956, 1970, 2196
Tru9I	TTAA	8 cut(s)	435, 461, 1034, 1061, 1761, 1956, 1970, 2196
TscAI	CASTG	4 cut(s)	82, 628, 738, 1401
TseFI	GTSAC	2 cut(s)	623, 2293
TseI	GCWGC	8 cut(s)	242, 687, 736, 1371, 1391, 1567, 2046, 2132
Tsp45I	GTSAC	2 cut(s)	623, 2293
TspGWI	ACGGA	2 cut(s)	704, 1368
TspRI	CASTG	4 cut(s)	82, 628, 738, 1401
Van91I	CCANNNNNTGG	1 cut(s)	425
Vha464I	CTTAAG	1 cut(s)	2195
VpaK11BI	GGWCC	2 cut(s)	1262, 1984
XapI	RAATTY	4 cut(s)	790, 1966, 2140, 2190
XbaI	TCTAGA	1 cut(s)	812
XceI	RCATGY	4 cut(s)	433, 633, 971, 1506
XcmI	CCANNNNNNNNNTGG	1 cut(s)	889
XhoI	CTCGAG	1 cut(s)	935
XmiI	GTMKAC	1 cut(s)	1527
XmnI	GAANNNNTTC	1 cut(s)	36
XspI	CTAG	5 cut(s)	663, 813, 1017, 1523, 1709
Zsp2I	ATGCAT	1 cut(s)	1221

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.