AT4G26550

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007812
Physical Location & Seq
Reverse (-)
13402379 .. 13404385
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G26550.1

Sequence Viewer

Length: 678 bp
ATGTCACAAGGATGGTTTTCGATGGGCGGGAGTAGCACCGTAGATCAGCAGCAGCAATCGGGTTCGTCTCTGCTTGCGGATTGGAATTCTTACGCAGCCTCTAGAGATTTCGAGGAAAGCGGCGGAAGCTTCGGCTTTGATATCGAATCCGCCGTTAGATCTGCCAATGACACTGTTTCGGGCACCTTCAGTGTGGTTTCAAAAGGAGTTAGAGATATTCCAGGGAGCTTGCAGTCAGCAACAAGTAGTATGCCTTCAGGAAAAGCGCTCATGTATTTCGGTTTACTTCTCGCAAGTGGTGTTTTCTTCATCTTCATTGCCTTCACAATGTTTCTTCCAGTCATGGTGCTTATGCCACAGAAATTTGCCATTTGTTTCACCCTTGGATGTGGATTCATCATTGGATCCTTCTTCGCACTTCGTGGCCCTAAAAATCAGCTCGCACACATGTCTTCGATGGAGAGGCTTCCTTCAACCCTAGGTTTCATTGCTACCATGGTTGGTACCATATACGTATCAATGGTTCTCCACAGCTACATTCTCTCGGTGCTCTTCTCAGTGTTACAGGTTCTTGCGCTGGTTTACTACTGTATATCCTACTTCCCTGGTGGATCATCAGGCATGAGGTTCCTCAGTTCTGCTCTCACCTCATCTGCGCTAAGAGTTTTCGGGAGATAA

Protein Analysis

225

Amino Acids

24.14

Weight (kDa)

8.49

Isoelectric Point (pI)

50.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 90 - 202 1.9e-27 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 503
AccB1I GGYRCC 2 cut(s) 182, 503
AciI CCGC 5 cut(s) 27, 77, 120, 123, 150
AclWI GGATC 3 cut(s) 399, 412, 619
AcsI RAATTY 2 cut(s) 85, 362
AcuI CTGAAG 2 cut(s) 172, 240
AdeI CACNNNGTG 1 cut(s) 422
AfaI GTAC 1 cut(s) 505
AfeI AGCGCT 1 cut(s) 267
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 2 cut(s) 201, 474
AjnI CCWGG 2 cut(s) 220, 604
AluBI AGCT 4 cut(s) 129, 228, 439, 534
AluI AGCT 4 cut(s) 129, 228, 439, 534
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 3 cut(s) 399, 412, 619
Aor51HI AGCGCT 1 cut(s) 267
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 3 cut(s) 49, 52, 95
ApoI RAATTY 2 cut(s) 85, 362
Asp718I GGTACC 1 cut(s) 503
AspA2I CCTAGG 1 cut(s) 478
AspLEI GCGC 3 cut(s) 268, 577, 658
AspS9I GGNCC 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 370, 637
AvrII CCTAGG 1 cut(s) 478
BaeGI GKGCMC 1 cut(s) 185
BamHI GGATCC 1 cut(s) 404
BanI GGYRCC 2 cut(s) 182, 503
BarI GAAGNNNNNNTAC 2 cut(s) 238, 270
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 3 cut(s) 61, 64, 107
BccI CCATC 3 cut(s) 6, 16, 451
BceAI ACGGC 1 cut(s) 137
BciT130I CCWGG 2 cut(s) 222, 606
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 2 cut(s) 102, 479
BfoI RGCGCY 1 cut(s) 269
BglII AGATCT 1 cut(s) 158
BisI GCNGC 4 cut(s) 50, 53, 96, 121
BlnI CCTAGG 1 cut(s) 478
BlsI GCNGC 4 cut(s) 51, 54, 97, 122
Bme1390I CCNGG 2 cut(s) 222, 606
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 4 cut(s) 184, 406, 505, 629
BmrFI CCNGG 2 cut(s) 222, 606
BpiI GAAGAC 1 cut(s) 444
BsaAI YACGTR 1 cut(s) 514
BsaJI CCNNGG 5 cut(s) 221, 382, 478, 495, 604
Bse1I ACTGG 1 cut(s) 338
Bse3DI GCAATG 2 cut(s) 315, 486
BseBI CCWGG 2 cut(s) 222, 606
BseDI CCNNGG 5 cut(s) 221, 382, 478, 495, 604
BseGI GGATG 2 cut(s) 17, 392
BseMI GCAATG 2 cut(s) 315, 486
BseMII CTCAG 2 cut(s) 570, 646
BseNI ACTGG 1 cut(s) 338
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 3 cut(s) 61, 64, 107
BshFI GGCC 1 cut(s) 426
BshNI GGYRCC 2 cut(s) 182, 503
BsiHKAI GWGCWC 1 cut(s) 552
BsmAI GTCTC 1 cut(s) 72
BsmBI CGTCTC 1 cut(s) 72
BsnI GGCC 1 cut(s) 426
Bsp1286I GDGCHC 2 cut(s) 185, 552
Bsp143I GATC 4 cut(s) 43, 158, 404, 611
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 5 cut(s) 27, 77, 120, 123, 150
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 2 cut(s) 569, 645
BspLI GGNNCC 4 cut(s) 184, 406, 505, 629
BspPI GGATC 3 cut(s) 399, 412, 619
BspQI GCTCTTC 1 cut(s) 557
BspT107I GGYRCC 2 cut(s) 182, 503
BsrDI GCAATG 2 cut(s) 315, 486
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 5 cut(s) 221, 382, 478, 495, 604
BssMI GATC 4 cut(s) 43, 158, 404, 611
BssT1I CCWWGG 3 cut(s) 382, 478, 495
Bst2UI CCWGG 2 cut(s) 222, 606
Bst4CI ACNGT 3 cut(s) 40, 175, 590
Bst6I CTCTTC 1 cut(s) 557
BstBAI YACGTR 1 cut(s) 514
BstC8I GCNNGC 3 cut(s) 75, 230, 441
BstDEI CTNAG 3 cut(s) 556, 632, 659
BstDSI CCRYGG 1 cut(s) 495
BstF5I GGATG 2 cut(s) 17, 392
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 3 cut(s) 268, 577, 658
BstKTI GATC 4 cut(s) 46, 161, 407, 614
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 4 cut(s) 43, 158, 404, 611
BstMWI GCNNNNNNNGC 2 cut(s) 33, 126
BstNI CCWGG 2 cut(s) 222, 606
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 2 cut(s) 220, 604
BstSLI GKGCMC 1 cut(s) 185
BstSNI TACGTA 1 cut(s) 514
BstV1I GCAGC 3 cut(s) 61, 64, 107
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 2 cut(s) 158, 404
BstYI RGATCY 2 cut(s) 158, 404
BsuRI GGCC 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 495
BtsCI GGATG 2 cut(s) 17, 392
BtsIMutI CAGTG 3 cut(s) 171, 196, 564
Cac8I GCNNGC 3 cut(s) 75, 230, 441
CfoI GCGC 3 cut(s) 268, 577, 658
Cfr13I GGNCC 1 cut(s) 425
Csp6I GTAC 1 cut(s) 504
CviAII CATG 5 cut(s) 271, 343, 448, 496, 622
CviJI RGCY 8 cut(s) 98, 129, 135, 228, 426, 439, 466, 534
CviKI_1 RGCY 8 cut(s) 98, 129, 135, 228, 426, 439, 466, 534
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 3 cut(s) 556, 632, 659
DpnI GATC 4 cut(s) 45, 160, 406, 613
DpnII GATC 4 cut(s) 43, 158, 404, 611
DraIII CACNNNGTG 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
EciI GGCGGA 2 cut(s) 138, 139
Eco105I TACGTA 1 cut(s) 514
Eco130I CCWWGG 3 cut(s) 382, 478, 495
Eco32I GATATC 1 cut(s) 142
Eco47III AGCGCT 1 cut(s) 267
Eco57I CTGAAG 2 cut(s) 172, 240
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 220, 604
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 3 cut(s) 382, 478, 495
ErhI CCWWGG 3 cut(s) 382, 478, 495
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 5 cut(s) 274, 346, 451, 499, 625
FatI CATG 5 cut(s) 270, 342, 447, 495, 621
FauI CCCGC 1 cut(s) 20
Fnu4HI GCNGC 4 cut(s) 50, 53, 96, 121
FokI GGATG 2 cut(s) 24, 399
Fsp4HI GCNGC 4 cut(s) 50, 53, 96, 121
FspBI CTAG 2 cut(s) 102, 479
GlaI GCGC 3 cut(s) 267, 576, 657
GluI GCNGC 4 cut(s) 50, 53, 96, 121
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 1 cut(s) 426
HhaI GCGC 3 cut(s) 268, 577, 658
Hin1II CATG 5 cut(s) 274, 346, 451, 499, 625
Hin6I GCGC 3 cut(s) 266, 575, 656
HinP1I GCGC 3 cut(s) 266, 575, 656
HindIII AAGCTT 1 cut(s) 127
HinfI GANTC 2 cut(s) 146, 393
HphI GGTGA 2 cut(s) 370, 637
Hpy166II GTNNAC 2 cut(s) 284, 583
Hpy188III TCNNGA 3 cut(s) 102, 258, 670
Hpy8I GTNNAC 2 cut(s) 284, 583
HpyAV CCTTC 5 cut(s) 196, 264, 331, 418, 480
HpyCH4III ACNGT 3 cut(s) 40, 175, 590
HpyCH4IV ACGT 1 cut(s) 513
HpyCH4V TGCA 1 cut(s) 232
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 126
HpyF3I CTNAG 3 cut(s) 556, 632, 659
HpySE526I ACGT 1 cut(s) 513
Hsp92II CATG 5 cut(s) 274, 346, 451, 499, 625
HspAI GCGC 3 cut(s) 266, 575, 656
KpnI GGTACC 1 cut(s) 507
Kzo9I GATC 4 cut(s) 43, 158, 404, 611
LguI GCTCTTC 1 cut(s) 557
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 9 cut(s) 207, 234, 243, 351, 551, 563, 591, 603, 618
Lsp1109I GCAGC 3 cut(s) 61, 64, 107
MaeI CTAG 2 cut(s) 102, 479
MaeII ACGT 1 cut(s) 513
MaeIII GTNAC 2 cut(s) 3, 561
MalI GATC 4 cut(s) 45, 160, 406, 613
MboI GATC 4 cut(s) 43, 158, 404, 611
MboII GAAGA 6 cut(s) 298, 304, 326, 403, 444, 544
MflI RGATCY 2 cut(s) 158, 404
MhlI GDGCHC 2 cut(s) 185, 552
MluCI AATT 2 cut(s) 85, 362
MnlI CCTC 6 cut(s) 106, 109, 456, 618, 641, 658
MslI CAYNNNNRTG 1 cut(s) 10
MspR9I CCNGG 2 cut(s) 222, 606
MvaI CCWGG 2 cut(s) 222, 606
MwoI GCNNNNNNNGC 2 cut(s) 33, 126
NcoI CCATGG 1 cut(s) 495
NdeII GATC 4 cut(s) 43, 158, 404, 611
NlaIII CATG 5 cut(s) 274, 346, 451, 499, 625
NlaIV GGNNCC 4 cut(s) 184, 406, 505, 629
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 451
PciI ACATGT 1 cut(s) 447
PciSI GCTCTTC 1 cut(s) 557
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PfeI GAWTC 2 cut(s) 146, 393
PkrI GCNGC 4 cut(s) 51, 54, 97, 122
Ppu21I YACGTR 1 cut(s) 514
PscI ACATGT 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 220, 604
PspGI CCWGG 2 cut(s) 220, 604
PspN4I GGNNCC 4 cut(s) 184, 406, 505, 629
PspPI GGNCC 1 cut(s) 425
PsrI GAACNNNNNNTAC 2 cut(s) 507, 539
PsuI RGATCY 2 cut(s) 158, 404
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
RseI CAYNNNNRTG 1 cut(s) 10
SapI GCTCTTC 1 cut(s) 557
SatI GCNGC 4 cut(s) 50, 53, 96, 121
Sau3AI GATC 4 cut(s) 43, 158, 404, 611
Sau96I GGNCC 1 cut(s) 425
ScrFI CCNGG 2 cut(s) 222, 606
SduI GDGCHC 2 cut(s) 185, 552
SmiMI CAYNNNNRTG 1 cut(s) 10
SnaBI TACGTA 1 cut(s) 514
Sse9I AATT 2 cut(s) 85, 362
SsiI CCGC 5 cut(s) 27, 77, 120, 123, 150
SspMI CTAG 2 cut(s) 102, 479
StyD4I CCNGG 2 cut(s) 220, 604
StyI CCWWGG 3 cut(s) 382, 478, 495
TaaI ACNGT 3 cut(s) 40, 175, 590
TaiI ACGT 1 cut(s) 516
TaqI TCGA 4 cut(s) 20, 111, 144, 455
TasI AATT 2 cut(s) 85, 362
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 2 cut(s) 146, 393
TscAI CASTG 3 cut(s) 178, 196, 564
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 49, 52, 95
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 4 cut(s) 298, 304, 385, 475
TspRI CASTG 3 cut(s) 178, 196, 564
XapI RAATTY 2 cut(s) 85, 362
XbaI TCTAGA 1 cut(s) 101
XceI RCATGY 1 cut(s) 451
XmaJI CCTAGG 1 cut(s) 478
XspI CTAG 2 cut(s) 102, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.