MD03G1036800.v1.1

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007812
Physical Location & Seq
Forward (+)
2918215 .. 2921084
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1036800.v1.1.491

Sequence Viewer

Length: 690 bp
ATGCAGAAGATGGCGCAGGAATGGTTTTCGCAGACCGGCAGCGGCGTCGACGACTCGCAGAAGCCGTCGTCGTCTTCTTTGCTAGCCGATTGGAACTCCTACGCCGCTTCACAATCCACTGACGAGAGCTCCTCTTTGGGACTCGGATTCGATCTGGAATCTGCGGTGCGCTCTGCTAACGACACCGTTTCCGGCACTTTCAGCGTGGTTTCAAAAGGAGTGAGAGATATACCTGGGAGCCTCCAGTCTGCTACTAGTAATGTCCCTTCAGGAAAAGCTCTCATGTATTTCGGCTTGTTTCTAGCCACTGGGGTGTTCTTTGTTTTTATCGCATTTACCATGTTCCTTCCTGTCATGGTGTTGATGCCTCAGAAGTTTGCTATCTGCTTTACACTTGGGTGTGCCTTTATTATCGCATCATTCTTTGCACTCAAAGGTCCAAAGAATCAGCTTGCTCACATGTCTTCAAAAGAGAGACTTCCGTTTACCGTTGGATTTTTAGGCACTATGGTGGGTACCATATATGTATCCATGGTACTCCACAGTTACATCCTCTCTGTTTTCTTCTCTGTGTTACAGGTTCTGGCACTTGCATACTATGCTGTTTCATACTTTCCTGGTGGATCTGCGGGTATGAAGTTCCTATCTTCTGCCCTTGCGTCTTCAGTAATGCGATGTTTTGGGAGGTGA

Protein Analysis

230

Amino Acids

24.55

Weight (kDa)

8.77

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 94 - 206 3.6e-29 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 515
AccI GTMKAC 1 cut(s) 48
AciI CCGC 4 cut(s) 42, 105, 164, 629
AclWI GGATC 1 cut(s) 631
AcuI CTGAAG 2 cut(s) 252, 648
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 517, 537
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 2 cut(s) 213, 468
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 2 cut(s) 232, 616
AleI CACNNNNGTG 3 cut(s) 311, 397, 509
AluBI AGCT 3 cut(s) 129, 278, 451
AluI AGCT 3 cut(s) 129, 278, 451
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 1 cut(s) 631
AlwNI CAGNNNCTG 1 cut(s) 583
ApeKI GCWGC 1 cut(s) 39
Asp718I GGTACC 1 cut(s) 515
AspLEI GCGC 2 cut(s) 16, 171
AspS9I GGNCC 1 cut(s) 437
AsuNHI GCTAGC 1 cut(s) 82
AvaII GGWCC 1 cut(s) 437
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 131
BarI GAAGNNNNNNTAC 2 cut(s) 250, 282
BbsI GAAGAC 3 cut(s) 66, 456, 654
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 51
BccI CCATC 1 cut(s) 4
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 2 cut(s) 234, 618
BciVI GTATCC 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 469
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 3 cut(s) 83, 255, 302
BfuI GTATCC 1 cut(s) 538
BisI GCNGC 3 cut(s) 40, 43, 105
BlsI GCNGC 3 cut(s) 41, 44, 106
Bme1390I CCNGG 2 cut(s) 234, 618
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 2 cut(s) 239, 517
BmrFI CCNGG 2 cut(s) 234, 618
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 2 cut(s) 354, 425
BmtI GCTAGC 1 cut(s) 86
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 3 cut(s) 66, 456, 654
BplI GAGNNNNNCTC 2 cut(s) 116, 148
BpmI CTGGAG 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 233, 531
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 2 cut(s) 244, 313
BseBI CCWGG 2 cut(s) 234, 618
BseDI CCNNGG 2 cut(s) 233, 531
BseGI GGATG 1 cut(s) 549
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 2 cut(s) 244, 313
BseRI GAGGAG 1 cut(s) 121
BseXI GCAGC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 131
BsiSI CCGG 2 cut(s) 36, 192
BslFI GGGAC 2 cut(s) 153, 248
BsmAI GTCTC 1 cut(s) 469
BsmFI GGGAC 2 cut(s) 153, 248
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 2 cut(s) 151, 623
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 4 cut(s) 42, 105, 164, 629
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 239, 517
BspOI GCTAGC 1 cut(s) 86
BspPI GGATC 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 515
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 2 cut(s) 244, 313
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 233, 531
BssMI GATC 2 cut(s) 151, 623
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 2 cut(s) 234, 618
Bst4CI ACNGT 3 cut(s) 187, 490, 545
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 84, 453
BstDEI CTNAG 1 cut(s) 369
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 1 cut(s) 549
BstHHI GCGC 2 cut(s) 16, 171
BstKTI GATC 2 cut(s) 154, 626
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 2 cut(s) 151, 623
BstMWI GCNNNNNNNGC 2 cut(s) 201, 599
BstNI CCWGG 2 cut(s) 234, 618
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 2 cut(s) 232, 616
BstV1I GCAGC 1 cut(s) 51
BstV2I GAAGAC 3 cut(s) 66, 456, 654
BstX2I RGATCY 1 cut(s) 623
BstYI RGATCY 1 cut(s) 623
BsuI GTATCC 1 cut(s) 538
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 1 cut(s) 549
BtsIMutI CAGTG 2 cut(s) 117, 306
Cac8I GCNNGC 2 cut(s) 84, 453
CaiI CAGNNNCTG 1 cut(s) 583
CfoI GCGC 2 cut(s) 16, 171
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 437
CseI GACGC 2 cut(s) 34, 648
Csp6I GTAC 2 cut(s) 516, 536
CviAII CATG 5 cut(s) 283, 340, 355, 460, 532
CviJI RGCY 8 cut(s) 64, 86, 129, 240, 278, 294, 305, 451
CviKI_1 RGCY 8 cut(s) 64, 86, 129, 240, 278, 294, 305, 451
CviQI GTAC 2 cut(s) 516, 536
DdeI CTNAG 1 cut(s) 369
DpnI GATC 2 cut(s) 153, 625
DpnII GATC 2 cut(s) 151, 623
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 252, 648
EcoICRI GAGCTC 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 232, 616
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 5 cut(s) 286, 343, 358, 463, 535
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 2 cut(s) 153, 248
FatI CATG 5 cut(s) 282, 339, 354, 459, 531
FauI CCCGC 1 cut(s) 622
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 40, 43, 105
FokI GGATG 1 cut(s) 536
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 40, 43, 105
FspBI CTAG 3 cut(s) 83, 255, 302
GlaI GCGC 2 cut(s) 15, 170
GluI GCNGC 3 cut(s) 40, 43, 105
GsuI CTGGAG 1 cut(s) 227
HapII CCGG 2 cut(s) 36, 192
HgaI GACGC 2 cut(s) 34, 648
HhaI GCGC 2 cut(s) 16, 171
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 5 cut(s) 286, 343, 358, 463, 535
Hin6I GCGC 2 cut(s) 14, 169
HinP1I GCGC 2 cut(s) 14, 169
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 5 cut(s) 53, 141, 147, 158, 445
HpaII CCGG 2 cut(s) 36, 192
Hpy166II GTNNAC 2 cut(s) 49, 486
Hpy188I TCNGA 2 cut(s) 146, 372
Hpy188III TCNNGA 2 cut(s) 155, 270
Hpy8I GTNNAC 2 cut(s) 49, 486
Hpy99I CGWCG 4 cut(s) 50, 53, 70, 73
HpyAV CCTTC 2 cut(s) 276, 356
HpyCH4III ACNGT 3 cut(s) 187, 490, 545
HpyCH4V TGCA 3 cut(s) 4, 428, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 599
HpyF3I CTNAG 1 cut(s) 369
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 5 cut(s) 286, 343, 358, 463, 535
HspAI GCGC 2 cut(s) 14, 169
KpnI GGTACC 1 cut(s) 519
Kzo9I GATC 2 cut(s) 151, 623
LmnI GCTCC 2 cut(s) 134, 237
Lsp1109I GCAGC 1 cut(s) 51
LweI GCATC 2 cut(s) 354, 425
MaeI CTAG 3 cut(s) 83, 255, 302
MaeIII GTNAC 2 cut(s) 545, 573
MalI GATC 2 cut(s) 153, 625
MboI GATC 2 cut(s) 151, 623
MboII GAAGA 6 cut(s) 19, 66, 456, 556, 639, 654
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 1 cut(s) 131
MlyI GAGTC 2 cut(s) 47, 135
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 5 cut(s) 142, 251, 378, 563, 678
MslI CAYNNNNRTG 3 cut(s) 311, 397, 509
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 2 cut(s) 36, 192
MspR9I CCNGG 2 cut(s) 234, 618
MvaI CCWGG 2 cut(s) 234, 618
MwoI GCNNNNNNNGC 2 cut(s) 201, 599
NcoI CCATGG 1 cut(s) 531
NdeII GATC 2 cut(s) 151, 623
NheI GCTAGC 1 cut(s) 82
NlaIII CATG 5 cut(s) 286, 343, 358, 463, 535
NlaIV GGNNCC 2 cut(s) 239, 517
NspI RCATGY 1 cut(s) 463
OliI CACNNNNGTG 3 cut(s) 311, 397, 509
PciI ACATGT 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PfeI GAWTC 3 cut(s) 147, 158, 445
PkrI GCNGC 3 cut(s) 41, 44, 106
PleI GAGTC 2 cut(s) 47, 135
PpsI GAGTC 2 cut(s) 47, 135
PscI ACATGT 1 cut(s) 459
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 232, 616
PspGI CCWGG 2 cut(s) 232, 616
PspN4I GGNNCC 2 cut(s) 239, 517
PspPI GGNCC 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 583
PsuI RGATCY 1 cut(s) 623
RsaI GTAC 2 cut(s) 517, 537
RsaNI GTAC 2 cut(s) 516, 536
RseI CAYNNNNRTG 3 cut(s) 311, 397, 509
SacI GAGCTC 1 cut(s) 131
SalI GTCGAC 1 cut(s) 47
SatI GCNGC 3 cut(s) 40, 43, 105
Sau3AI GATC 2 cut(s) 151, 623
Sau96I GGNCC 1 cut(s) 437
SchI GAGTC 2 cut(s) 47, 135
ScrFI CCNGG 2 cut(s) 234, 618
SduI GDGCHC 1 cut(s) 131
SetI ASST 7 cut(s) 131, 235, 280, 439, 453, 582, 689
SfaNI GCATC 2 cut(s) 354, 425
SgrDI CGTCGACG 1 cut(s) 47
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 3 cut(s) 311, 397, 509
SpeI ACTAGT 1 cut(s) 254
SsiI CCGC 4 cut(s) 42, 105, 164, 629
SspMI CTAG 3 cut(s) 83, 255, 302
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 232, 616
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 3 cut(s) 187, 490, 545
TaqI TCGA 2 cut(s) 48, 150
TauI GCSGC 2 cut(s) 45, 107
TfiI GAWTC 3 cut(s) 147, 158, 445
TscAI CASTG 2 cut(s) 124, 313
TseI GCWGC 1 cut(s) 39
TspDTI ATGAA 2 cut(s) 597, 650
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 2 cut(s) 124, 313
VpaK11BI GGWCC 1 cut(s) 437
XceI RCATGY 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 3 cut(s) 83, 255, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.