Rh5DG531100

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007812
Physical Location & Seq
Forward (+)
81896189 .. 81904946
8758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG531100.1

Sequence Viewer

Length: 690 bp
ATGCAGAAAATGGCGCAGGAATGGTTCTCCGGCGGCAGCACCGGCGCCGGCGGCGACTCTCAGCAGCCGGCGTCTTCTCTCTTAGCCGATTGGAACTCCTACGCCGCTTCCCAATCCGCAGAGGACAGCTCCTCTTCGGGCCTCAGCTTCGATCTCGAATCCGCGGTCCGCTCCGCAAACGACACCGTTTCAGGCACTTTCAGCGTGGTTTCCAAAGGAGTTAGAGATCTACCTGGGAACCTCCAGTCTGCCACTAGTAGTGTCCCTTCAGGAAAAGCTCTCATGTATTTTGGTTTGTTTCTGGCAACTGGGGTGTTCTTCGTTTTTATTGCATTTACCATGTTCCTTCCGGTTATGGTATTGATGCCACAGAAGTTTGCTATCTGCTTTACACTCGGGTGTGCCTTTATAATTGGATCCTTCTTTGCACTTAAAGGTCCAAAGAATCAGTTTGTTCACATGTCCTCAAAAGAGAGACTTCCTTTCACACTTGGATTTTTAGGCAGTATGGTGGGTACCATATATGTCTCGATGGTGCTTCACAGCTATATTCTCTCTGTGTTTTTCTCTGTATTACAGGTTCTGGCGCTTGCATACTATGCTGTTTCATACTTTCCTGGTGGATCAGCGGGTTTGAAGTTCCTATCTTCTGCCCTTACGTCTTCAGTAATGAAATGTTTTGGGAGGTGA

Protein Analysis

229

Amino Acids

24.36

Weight (kDa)

8.77

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 94 - 206 6.2e-29 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 410
Acc65I GGTACC 1 cut(s) 515
AccB1I GGYRCC 2 cut(s) 44, 515
AccBSI CCGCTC 1 cut(s) 171
AccII CGCG 1 cut(s) 164
AciI CCGC 9 cut(s) 33, 51, 105, 117, 162, 164, 169, 174, 629
AclWI GGATC 3 cut(s) 411, 424, 631
AcuI CTGAAG 2 cut(s) 252, 648
AcyI GRCGYC 2 cut(s) 45, 71
AfaI GTAC 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 1 cut(s) 637
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 2 cut(s) 232, 616
AleI CACNNNNGTG 1 cut(s) 397
AluBI AGCT 4 cut(s) 129, 147, 278, 546
AluI AGCT 4 cut(s) 129, 147, 278, 546
Alw26I GTCTC 2 cut(s) 469, 532
AlwI GGATC 3 cut(s) 411, 424, 631
AlwNI CAGNNNCTG 1 cut(s) 583
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 36, 64
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
Asp718I GGTACC 1 cut(s) 515
AspLEI GCGC 3 cut(s) 16, 47, 589
AspS9I GGNCC 3 cut(s) 139, 166, 437
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 2 cut(s) 166, 437
BamHI GGATCC 1 cut(s) 416
BanI GGYRCC 2 cut(s) 44, 515
BarI GAAGNNNNNNTAC 2 cut(s) 250, 282
BbsI GAAGAC 2 cut(s) 66, 654
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 2 cut(s) 48, 76
BccI CCATC 1 cut(s) 526
BciT130I CCWGG 2 cut(s) 234, 618
BcoDI GTCTC 2 cut(s) 469, 532
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 1 cut(s) 255
BfoI RGCGCY 2 cut(s) 48, 590
BglII AGATCT 1 cut(s) 226
BisI GCNGC 5 cut(s) 34, 37, 52, 65, 105
BlsI GCNGC 5 cut(s) 35, 38, 53, 66, 106
Bme1390I CCNGG 2 cut(s) 234, 618
Bme18I GGWCC 2 cut(s) 166, 437
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 3 cut(s) 139, 166, 437
BmiI GGNNCC 4 cut(s) 46, 239, 418, 517
BmrFI CCNGG 2 cut(s) 234, 618
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 354
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 2 cut(s) 66, 654
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 1 cut(s) 143
BsaHI GRCGYC 2 cut(s) 45, 71
BsaJI CCNNGG 2 cut(s) 162, 233
BsaWI WCCGGW 1 cut(s) 349
Bse118I RCCGGY 3 cut(s) 41, 47, 67
Bse1I ACTGG 2 cut(s) 244, 313
BseBI CCWGG 2 cut(s) 234, 618
BseDI CCNNGG 2 cut(s) 162, 233
BseMII CTCAG 2 cut(s) 74, 157
BseNI ACTGG 2 cut(s) 244, 313
BseRI GAGGAG 1 cut(s) 121
BseXI GCAGC 2 cut(s) 48, 76
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 1 cut(s) 141
BshNI GGYRCC 2 cut(s) 44, 515
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 5 cut(s) 30, 42, 48, 68, 350
BslFI GGGAC 1 cut(s) 248
BsmAI GTCTC 2 cut(s) 469, 532
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 1 cut(s) 141
BsoBI CYCGRG 1 cut(s) 395
Bsp143I GATC 4 cut(s) 151, 226, 416, 623
BspACI CCGC 9 cut(s) 33, 51, 105, 117, 162, 164, 169, 174, 629
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 73, 156
BspFNI CGCG 1 cut(s) 164
BspLI GGNNCC 4 cut(s) 46, 239, 418, 517
BspPI GGATC 3 cut(s) 411, 424, 631
BspT107I GGYRCC 2 cut(s) 44, 515
BsrBI CCGCTC 1 cut(s) 171
BsrFI RCCGGY 3 cut(s) 41, 47, 67
BsrI ACTGG 2 cut(s) 244, 313
BssAI RCCGGY 3 cut(s) 41, 47, 67
BssECI CCNNGG 2 cut(s) 162, 233
BssMI GATC 4 cut(s) 151, 226, 416, 623
BssNI GRCGYC 2 cut(s) 45, 71
Bst2UI CCWGG 2 cut(s) 234, 618
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 139
BstACI GRCGYC 2 cut(s) 45, 71
BstAPI GCANNNNNTGC 1 cut(s) 599
BstC8I GCNNGC 3 cut(s) 49, 69, 591
BstDEI CTNAG 3 cut(s) 60, 82, 143
BstDSI CCRYGG 1 cut(s) 162
BstFNI CGCG 1 cut(s) 164
BstH2I RGCGCY 2 cut(s) 48, 590
BstHHI GCGC 3 cut(s) 16, 47, 589
BstKTI GATC 4 cut(s) 154, 229, 419, 626
BstMAI GTCTC 2 cut(s) 469, 532
BstMBI GATC 4 cut(s) 151, 226, 416, 623
BstMWI GCNNNNNNNGC 4 cut(s) 42, 51, 201, 599
BstNI CCWGG 2 cut(s) 234, 618
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 2 cut(s) 232, 616
BstUI CGCG 1 cut(s) 164
BstV1I GCAGC 2 cut(s) 48, 76
BstV2I GAAGAC 2 cut(s) 66, 654
BstX2I RGATCY 2 cut(s) 226, 416
BstYI RGATCY 2 cut(s) 226, 416
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 162
Cac8I GCNNGC 3 cut(s) 49, 69, 591
CaiI CAGNNNCTG 1 cut(s) 583
CfoI GCGC 3 cut(s) 16, 47, 589
Cfr10I RCCGGY 3 cut(s) 41, 47, 67
Cfr13I GGNCC 3 cut(s) 139, 166, 437
Cfr42I CCGCGG 1 cut(s) 165
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 1 cut(s) 60
Csp6I GTAC 1 cut(s) 516
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 3 cut(s) 283, 340, 460
CviJI RGCY 7 cut(s) 67, 86, 129, 141, 147, 278, 546
CviKI_1 RGCY 7 cut(s) 67, 86, 129, 141, 147, 278, 546
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 3 cut(s) 60, 82, 143
DinI GGCGCC 1 cut(s) 46
DpnI GATC 4 cut(s) 153, 228, 418, 625
DpnII GATC 4 cut(s) 151, 226, 416, 623
Eam1104I CTCTTC 1 cut(s) 139
EarI CTCTTC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 166, 437
Eco57I CTGAAG 2 cut(s) 252, 648
Eco88I CYCGRG 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 232, 616
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 3 cut(s) 286, 343, 463
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 1 cut(s) 248
FatI CATG 3 cut(s) 282, 339, 459
FauI CCCGC 1 cut(s) 622
Fnu4HI GCNGC 5 cut(s) 34, 37, 52, 65, 105
Fsp4HI GCNGC 5 cut(s) 34, 37, 52, 65, 105
FspBI CTAG 1 cut(s) 255
GlaI GCGC 3 cut(s) 15, 46, 588
GluI GCNGC 5 cut(s) 34, 37, 52, 65, 105
GsuI CTGGAG 1 cut(s) 227
HaeII RGCGCY 2 cut(s) 48, 590
HaeIII GGCC 1 cut(s) 141
HapII CCGG 5 cut(s) 30, 42, 48, 68, 350
HgaI GACGC 1 cut(s) 60
HhaI GCGC 3 cut(s) 16, 47, 589
Hin1I GRCGYC 2 cut(s) 45, 71
Hin1II CATG 3 cut(s) 286, 343, 463
Hin6I GCGC 3 cut(s) 14, 45, 587
HinP1I GCGC 3 cut(s) 14, 45, 587
HinfI GANTC 3 cut(s) 56, 158, 445
HpaII CCGG 5 cut(s) 30, 42, 48, 68, 350
Hpy166II GTNNAC 1 cut(s) 457
Hpy188III TCNNGA 3 cut(s) 155, 270, 529
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 3 cut(s) 276, 356, 430
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 1 cut(s) 659
HpyCH4V TGCA 4 cut(s) 4, 332, 428, 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 51, 201, 599
HpyF3I CTNAG 3 cut(s) 60, 82, 143
HpySE526I ACGT 1 cut(s) 659
Hsp92I GRCGYC 2 cut(s) 45, 71
Hsp92II CATG 3 cut(s) 286, 343, 463
HspAI GCGC 3 cut(s) 14, 45, 587
KasI GGCGCC 1 cut(s) 44
KpnI GGTACC 1 cut(s) 519
KroI GCCGGC 2 cut(s) 47, 67
KroNI GCCGGC 2 cut(s) 49, 69
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 4 cut(s) 151, 226, 416, 623
LmnI GCTCC 2 cut(s) 134, 176
Lsp1109I GCAGC 2 cut(s) 48, 76
LweI GCATC 1 cut(s) 354
MaeI CTAG 1 cut(s) 255
MaeII ACGT 1 cut(s) 659
MalI GATC 4 cut(s) 153, 228, 418, 625
MbiI CCGCTC 1 cut(s) 171
MboI GATC 4 cut(s) 151, 226, 416, 623
MboII GAAGA 5 cut(s) 66, 126, 310, 639, 654
MflI RGATCY 2 cut(s) 226, 416
MluCI AATT 1 cut(s) 411
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 6 cut(s) 115, 142, 152, 251, 475, 678
MreI CGCCGGCG 1 cut(s) 47
MroNI GCCGGC 2 cut(s) 47, 67
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 1 cut(s) 397
MspA1I CMGCKG 2 cut(s) 164, 629
MspI CCGG 5 cut(s) 30, 42, 48, 68, 350
MspR9I CCNGG 2 cut(s) 234, 618
MvaI CCWGG 2 cut(s) 234, 618
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 4 cut(s) 42, 51, 201, 599
NaeI GCCGGC 2 cut(s) 49, 69
NarI GGCGCC 1 cut(s) 45
NdeII GATC 4 cut(s) 151, 226, 416, 623
NgoMIV GCCGGC 2 cut(s) 47, 67
NlaIII CATG 3 cut(s) 286, 343, 463
NlaIV GGNNCC 4 cut(s) 46, 239, 418, 517
NspI RCATGY 1 cut(s) 463
OliI CACNNNNGTG 1 cut(s) 397
PciI ACATGT 1 cut(s) 459
PdiI GCCGGC 2 cut(s) 49, 69
PfeI GAWTC 2 cut(s) 158, 445
PkrI GCNGC 5 cut(s) 35, 38, 53, 66, 106
PleI GAGTC 1 cut(s) 50
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 50
PscI ACATGT 1 cut(s) 459
PsiI TTATAA 1 cut(s) 410
Psp6I CCWGG 2 cut(s) 232, 616
PspGI CCWGG 2 cut(s) 232, 616
PspN4I GGNNCC 4 cut(s) 46, 239, 418, 517
PspPI GGNCC 3 cut(s) 139, 166, 437
PsrI GAACNNNNNNTAC 2 cut(s) 564, 596
PstNI CAGNNNCTG 1 cut(s) 583
PsuI RGATCY 2 cut(s) 226, 416
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
RseI CAYNNNNRTG 1 cut(s) 397
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SacII CCGCGG 1 cut(s) 165
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 5 cut(s) 34, 37, 52, 65, 105
Sau3AI GATC 4 cut(s) 151, 226, 416, 623
Sau96I GGNCC 3 cut(s) 139, 166, 437
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 2 cut(s) 234, 618
SfaNI GCATC 1 cut(s) 354
SfoI GGCGCC 1 cut(s) 46
Sfr303I CCGCGG 1 cut(s) 165
SgrAI CRCCGGYG 2 cut(s) 41, 47
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 2 cut(s) 166, 437
SmiMI CAYNNNNRTG 1 cut(s) 397
SpeI ACTAGT 1 cut(s) 254
Sse9I AATT 1 cut(s) 411
SsiI CCGC 9 cut(s) 33, 51, 105, 117, 162, 164, 169, 174, 629
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 232, 616
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 1 cut(s) 662
TaqI TCGA 3 cut(s) 150, 156, 530
TasI AATT 1 cut(s) 411
TauI GCSGC 3 cut(s) 36, 54, 107
TfiI GAWTC 2 cut(s) 158, 445
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseI GCWGC 2 cut(s) 36, 64
TspDTI ATGAA 2 cut(s) 597, 686
VpaK11BI GGWCC 2 cut(s) 166, 437
XceI RCATGY 1 cut(s) 463
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.