AT5G56020

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007812
Physical Location & Seq
Forward (+)
22684386 .. 22686466
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56020.1

Sequence Viewer

Length: 693 bp
ATGGAGAAAATGGCCAAAGGATGGTTTCCGATGAGCGGCACTAGTAGCAGCGGAGATCAGCAGCAGCAACCGCTGCAAACAGGAGGAACGTCTCTGCTAGCTGATTGGAATTCTTACGCAGCATCGAGAGATTTTGAAGAAGACACCGGAACTTTAGGCTTCGATATCGAATCCGCCGTTAGATCTGCCAACGACACTGTTTCCGGCACCTTTAATGTTGTTTCGAAAGGAGTTAGAGATAACCTGCAGTCTGCAACAAGTAGTATGCCTTCAGGAAAAGCTTTGATGTATTTCGGTTTACTTCTAGCAAGTGGTGTATTCTTCATCTTCATTGCCTTCACCATGTTTCTTCCAGTCATGGTGCTTATGCCACAGAAGTTTGCAATTTGTTTTACCCTTGGATGTGGATTTATCATCGGATCTTTCTTCGCTCTTCGTGGCCCGCAAAATCAACTCGCTCACATGTCATCGGCAGAGAGGCTTCCACTAACTTTAGGTTTCATTGCCACCATGGTTGGCACCATATATGTCTCGATGGTGCTCCATAGCTACATTCTCTCTGTGATTTTCTCTGCATTACAGGTTATTGCACTGGCGTACTACTGTATATCATATTTCCCTGGTGGATCATCGGGAATGAAGTTCCTCAGTTCTACTCTTACCTCCTCTGTCTTCCGAATGTTTGGGAGATGA

Protein Analysis

230

Amino Acids

24.87

Weight (kDa)

7.7

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 95 - 207 1.5e-26 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccB1I GGYRCC 2 cut(s) 206, 518
AccB7I CCANNNNNTGG 1 cut(s) 21
AccBSI CCGCTC 1 cut(s) 36
AciI CCGC 5 cut(s) 36, 51, 71, 174, 443
AclWI GGATC 2 cut(s) 427, 634
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 1 cut(s) 599
AfiI CCNNNNNNNGG 2 cut(s) 21, 35
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 1 cut(s) 137
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 1 cut(s) 619
AluBI AGCT 3 cut(s) 101, 281, 549
AluI AGCT 3 cut(s) 101, 281, 549
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 2 cut(s) 96, 535
AlwI GGATC 2 cut(s) 427, 634
AoxI GGCC 2 cut(s) 12, 439
ApeKI GCWGC 5 cut(s) 48, 61, 64, 73, 119
ApoI RAATTY 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 331
AsuII TTCGAA 1 cut(s) 224
AsuNHI GCTAGC 1 cut(s) 97
BalI TGGCCA 1 cut(s) 14
BanI GGYRCC 2 cut(s) 206, 518
BarI GAAGNNNNNNTAC 2 cut(s) 253, 285
BbsI GAAGAC 2 cut(s) 147, 664
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 5 cut(s) 60, 60, 73, 76, 131
BccI CCATC 2 cut(s) 15, 529
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 621
BcoDI GTCTC 2 cut(s) 96, 535
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 98, 305
BfmI CTRYAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 252
BglII AGATCT 1 cut(s) 182
BisI GCNGC 6 cut(s) 37, 49, 62, 65, 74, 120
BlsI GCNGC 6 cut(s) 38, 50, 63, 66, 75, 121
Bme1390I CCNGG 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 208, 520
BmrFI CCNGG 1 cut(s) 621
BmsI GCATC 1 cut(s) 131
BmtI GCTAGC 1 cut(s) 101
BpiI GAAGAC 2 cut(s) 147, 664
Bpu14I TTCGAA 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 397, 510, 619
BsaWI WCCGGW 1 cut(s) 146
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 35
Bse1I ACTGG 2 cut(s) 353, 597
Bse3DI GCAATG 2 cut(s) 330, 501
BseBI CCWGG 1 cut(s) 621
BseDI CCNNGG 3 cut(s) 397, 510, 619
BseGI GGATG 2 cut(s) 26, 407
BseLI CCNNNNNNNGG 2 cut(s) 21, 35
BseMI GCAATG 2 cut(s) 330, 501
BseMII CTCAG 1 cut(s) 661
BseNI ACTGG 2 cut(s) 353, 597
BseRI GAGGAG 1 cut(s) 655
BseXI GCAGC 5 cut(s) 60, 60, 73, 76, 131
BshFI GGCC 2 cut(s) 14, 441
BshNI GGYRCC 2 cut(s) 206, 518
BsiHKAI GWGCWC 1 cut(s) 543
BsiSI CCGG 2 cut(s) 147, 204
BslI CCNNNNNNNGG 2 cut(s) 21, 35
BsmAI GTCTC 2 cut(s) 96, 535
BsmBI CGTCTC 1 cut(s) 96
BsnI GGCC 2 cut(s) 14, 441
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 4 cut(s) 55, 182, 419, 626
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 5 cut(s) 36, 51, 71, 174, 443
BspANI GGCC 2 cut(s) 14, 441
BspCNI CTCAG 1 cut(s) 660
BspLI GGNNCC 2 cut(s) 208, 520
BspMAI CTGCAG 1 cut(s) 249
BspMI ACCTGC 1 cut(s) 252
BspOI GCTAGC 1 cut(s) 101
BspPI GGATC 2 cut(s) 427, 634
BspQI GCTCTTC 1 cut(s) 438
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 206, 518
BsrBI CCGCTC 1 cut(s) 36
BsrDI GCAATG 2 cut(s) 330, 501
BsrI ACTGG 2 cut(s) 353, 597
BssECI CCNNGG 3 cut(s) 397, 510, 619
BssMI GATC 4 cut(s) 55, 182, 419, 626
BssT1I CCWWGG 2 cut(s) 397, 510
Bst2UI CCWGG 1 cut(s) 621
Bst4CI ACNGT 2 cut(s) 199, 605
Bst6I CTCTTC 1 cut(s) 438
BstAPI GCANNNNNTGC 1 cut(s) 73
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 99, 443
BstDEI CTNAG 1 cut(s) 647
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 2 cut(s) 26, 407
BstKTI GATC 4 cut(s) 58, 185, 422, 629
BstMAI GTCTC 2 cut(s) 96, 535
BstMBI GATC 4 cut(s) 55, 182, 419, 626
BstMWI GCNNNNNNNGC 3 cut(s) 45, 70, 73
BstNI CCWGG 1 cut(s) 621
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 1 cut(s) 619
BstSFI CTRYAG 1 cut(s) 245
BstV1I GCAGC 5 cut(s) 60, 60, 73, 76, 131
BstV2I GAAGAC 2 cut(s) 147, 664
BstX2I RGATCY 2 cut(s) 182, 419
BstYI RGATCY 2 cut(s) 182, 419
BsuRI GGCC 2 cut(s) 14, 441
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 2 cut(s) 26, 407
BtsIMutI CAGTG 2 cut(s) 195, 590
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 99, 443
Cfr13I GGNCC 1 cut(s) 440
Csp6I GTAC 1 cut(s) 598
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviAII CATG 4 cut(s) 343, 358, 463, 511
CviJI RGCY 7 cut(s) 14, 101, 159, 281, 441, 481, 549
CviKI_1 RGCY 7 cut(s) 14, 101, 159, 281, 441, 481, 549
CviQI GTAC 1 cut(s) 598
DdeI CTNAG 1 cut(s) 647
DpnI GATC 4 cut(s) 57, 184, 421, 628
DpnII GATC 4 cut(s) 55, 182, 419, 626
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 397, 510
Eco32I GATATC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 255
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 619
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 2 cut(s) 397, 510
ErhI CCWWGG 2 cut(s) 397, 510
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 4 cut(s) 346, 361, 466, 514
FatI CATG 4 cut(s) 342, 357, 462, 510
FauI CCCGC 1 cut(s) 450
Fnu4HI GCNGC 6 cut(s) 37, 49, 62, 65, 74, 120
FokI GGATG 2 cut(s) 33, 414
Fsp4HI GCNGC 6 cut(s) 37, 49, 62, 65, 74, 120
FspBI CTAG 3 cut(s) 42, 98, 305
GluI GCNGC 6 cut(s) 37, 49, 62, 65, 74, 120
HaeIII GGCC 2 cut(s) 14, 441
HapII CCGG 2 cut(s) 147, 204
Hin1II CATG 4 cut(s) 346, 361, 466, 514
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 1 cut(s) 170
HpaII CCGG 2 cut(s) 147, 204
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 1 cut(s) 299
Hpy188I TCNGA 3 cut(s) 30, 419, 677
Hpy188III TCNNGA 4 cut(s) 126, 273, 532, 633
Hpy8I GTNNAC 1 cut(s) 299
HpyAV CCTTC 2 cut(s) 279, 346
HpyCH4III ACNGT 2 cut(s) 199, 605
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 6 cut(s) 76, 247, 254, 383, 575, 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 70, 73
HpyF3I CTNAG 1 cut(s) 647
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 4 cut(s) 346, 361, 466, 514
Kzo9I GATC 4 cut(s) 55, 182, 419, 626
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 1 cut(s) 546
Lsp1109I GCAGC 5 cut(s) 60, 60, 73, 76, 131
LweI GCATC 1 cut(s) 131
MaeI CTAG 3 cut(s) 42, 98, 305
MaeII ACGT 1 cut(s) 89
MalI GATC 4 cut(s) 57, 184, 421, 628
MbiI CCGCTC 1 cut(s) 36
MboI GATC 4 cut(s) 55, 182, 419, 626
MboII GAAGA 8 cut(s) 149, 152, 313, 319, 341, 418, 425, 664
MflI RGATCY 2 cut(s) 182, 419
MhlI GDGCHC 1 cut(s) 543
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 2 cut(s) 109, 384
MluNI TGGCCA 1 cut(s) 14
MnlI CCTC 5 cut(s) 77, 471, 656, 673, 676
Mox20I TGGCCA 1 cut(s) 14
MscI TGGCCA 1 cut(s) 14
MseI TTAA 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 14
MspA1I CMGCKG 2 cut(s) 51, 73
MspI CCGG 2 cut(s) 147, 204
MspR9I CCNGG 1 cut(s) 621
MvaI CCWGG 1 cut(s) 621
MwoI GCNNNNNNNGC 3 cut(s) 45, 70, 73
NcoI CCATGG 1 cut(s) 510
NdeII GATC 4 cut(s) 55, 182, 419, 626
NheI GCTAGC 1 cut(s) 97
NlaIII CATG 4 cut(s) 346, 361, 466, 514
NlaIV GGNNCC 2 cut(s) 208, 520
NspI RCATGY 1 cut(s) 466
NspV TTCGAA 1 cut(s) 224
PciI ACATGT 1 cut(s) 462
PciSI GCTCTTC 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 21
PkrI GCNGC 6 cut(s) 38, 50, 63, 66, 75, 121
PscI ACATGT 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 619
PspGI CCWGG 1 cut(s) 619
PspN4I GGNNCC 2 cut(s) 208, 520
PspPI GGNCC 1 cut(s) 440
PstI CTGCAG 1 cut(s) 249
PsuI RGATCY 2 cut(s) 182, 419
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 6 cut(s) 37, 49, 62, 65, 74, 120
Sau3AI GATC 4 cut(s) 55, 182, 419, 626
Sau96I GGNCC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 621
SduI GDGCHC 1 cut(s) 543
SetI ASST 9 cut(s) 92, 103, 212, 246, 283, 499, 551, 585, 665
SfaNI GCATC 1 cut(s) 131
SfcI CTRYAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 224
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 2 cut(s) 109, 384
SsiI CCGC 5 cut(s) 36, 51, 71, 174, 443
SspMI CTAG 3 cut(s) 42, 98, 305
StyD4I CCNGG 1 cut(s) 619
StyI CCWWGG 2 cut(s) 397, 510
TaaI ACNGT 2 cut(s) 199, 605
TaiI ACGT 1 cut(s) 92
TaqI TCGA 5 cut(s) 125, 162, 168, 224, 533
TasI AATT 2 cut(s) 109, 384
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 2 cut(s) 202, 597
TseI GCWGC 5 cut(s) 48, 61, 64, 73, 119
TspDTI ATGAA 4 cut(s) 313, 319, 490, 653
TspRI CASTG 2 cut(s) 202, 597
Van91I CCANNNNNTGG 1 cut(s) 21
XapI RAATTY 1 cut(s) 109
XceI RCATGY 1 cut(s) 466
XspI CTAG 3 cut(s) 42, 98, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.