MD17G1169900.v1.1

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007812
Physical Location & Seq
Forward (+)
17307446 .. 17310111
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1169900.v1.1.491

Sequence Viewer

Length: 633 bp
ATGCAGAATATGGCGCAGGAATGGTTTTCGCAGACAGGCAGCGGCGTCGATGACTCGCAGAAGCCGTCGTCGTCGTCCTTCTCCTCTTGGGGACTTGGATTCGATCTCGAATCTGCGGTCCGTTCTGCTAACGACACCGTTTCCGGAACTTTCAGCGTGGTTTCCAAAGCAGTGAGAGATATACCTGGGAGCCTCCAGTCTGCTACTAGTAATGTCCCTTCAGGAAAAGCTCTCATGTATTTTGGCTTGTTTCTAGCAACTGGGGTGTTCTTTGTTTTTATCGCATTTACCACGTTCCTTCCTGTCATGGTGTTGATGCCTCAGAAGTTTGCTATCTGCTTTACGCTTGGGTGTGCCTTTATTATGGCATCCTTCTTTGCACTCAAAGGTCCAAAGAATCAGCTTGCTCACATGTCTTCAAAAGAGAGACTTCCGTTTACTGTTGGATTTTTAGGCAGTATGGTGGGTACCATATATGTATCCATGGTGCTCCACAATTACATTCTCTCTGTTTTCTTCTCTGTGTTACAGGTTCTGGCACTTGCTTACTATGCTGTTTCGTACTTTCCTGGTGGATCTGCGGGTTTGAGTTCCTATCTTAAGTCTTCCGTAATGAGATGCTTTGGGTGGTGA

Protein Analysis

211

Amino Acids

22.71

Weight (kDa)

9.03

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 78 - 190 2.6e-29 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccIII TCCGGA 1 cut(s) 143
AciI CCGC 3 cut(s) 42, 116, 581
AclWI GGATC 1 cut(s) 583
AcuI CTGAAG 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 469, 563
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 1 cut(s) 420
AhlI ACTAGT 1 cut(s) 206
AjnI CCWGG 2 cut(s) 184, 568
AluBI AGCT 2 cut(s) 230, 403
AluI AGCT 2 cut(s) 230, 403
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 1 cut(s) 583
AlwNI CAGNNNCTG 1 cut(s) 535
Aor13HI TCCGGA 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 39
ArsI GACNNNNNNTTYG 2 cut(s) 102, 134
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 2 cut(s) 118, 389
AvaII GGWCC 2 cut(s) 118, 389
BanI GGYRCC 1 cut(s) 467
BarI GAAGNNNNNNTAC 2 cut(s) 202, 234
BbsI GAAGAC 2 cut(s) 408, 597
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 51
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 2 cut(s) 186, 570
BciVI GTATCC 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 421
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 2 cut(s) 207, 254
BfrI CTTAAG 1 cut(s) 599
BfuI GTATCC 1 cut(s) 490
BisI GCNGC 2 cut(s) 40, 43
BlsI GCNGC 2 cut(s) 41, 44
Bme1390I CCNGG 2 cut(s) 186, 570
Bme18I GGWCC 2 cut(s) 118, 389
BmgT120I GGNCC 2 cut(s) 118, 389
BmiI GGNNCC 2 cut(s) 191, 469
BmrFI CCNGG 2 cut(s) 186, 570
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 3 cut(s) 306, 377, 608
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 408, 597
BpmI CTGGAG 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 185, 483
BsaWI WCCGGW 1 cut(s) 143
Bse1I ACTGG 2 cut(s) 196, 265
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 2 cut(s) 186, 570
BseDI CCNNGG 2 cut(s) 185, 483
BseGI GGATG 1 cut(s) 368
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 2 cut(s) 196, 265
BseRI GAGGAG 1 cut(s) 73
BseXI GCAGC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 492
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 2 cut(s) 105, 200
BsmAI GTCTC 1 cut(s) 421
BsmFI GGGAC 2 cut(s) 105, 200
Bsp1286I GDGCHC 1 cut(s) 492
Bsp13I TCCGGA 1 cut(s) 143
Bsp143I GATC 2 cut(s) 103, 575
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 3 cut(s) 42, 116, 581
BspCNI CTCAG 1 cut(s) 334
BspEI TCCGGA 1 cut(s) 143
BspLI GGNNCC 2 cut(s) 191, 469
BspPI GGATC 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 467
BspTI CTTAAG 1 cut(s) 599
BsrI ACTGG 2 cut(s) 196, 265
BssECI CCNNGG 2 cut(s) 185, 483
BssMI GATC 2 cut(s) 103, 575
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 2 cut(s) 186, 570
Bst4CI ACNGT 2 cut(s) 139, 442
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 1 cut(s) 321
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 1 cut(s) 368
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 2 cut(s) 106, 578
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 2 cut(s) 103, 575
BstMWI GCNNNNNNNGC 1 cut(s) 551
BstNI CCWGG 2 cut(s) 186, 570
BstNSI RCATGY 1 cut(s) 415
BstSCI CCNGG 2 cut(s) 184, 568
BstV1I GCAGC 1 cut(s) 51
BstV2I GAAGAC 2 cut(s) 408, 597
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BsuI GTATCC 1 cut(s) 490
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 1 cut(s) 368
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 405
CaiI CAGNNNCTG 1 cut(s) 535
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 118, 389
CpoI CGGWCCG 1 cut(s) 118
CseI GACGC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 468, 562
CspI CGGWCCG 1 cut(s) 118
CviAII CATG 4 cut(s) 235, 307, 412, 484
CviJI RGCY 5 cut(s) 64, 192, 230, 246, 403
CviKI_1 RGCY 5 cut(s) 64, 192, 230, 246, 403
CviQI GTAC 2 cut(s) 468, 562
DdeI CTNAG 1 cut(s) 321
DpnI GATC 2 cut(s) 105, 577
DpnII GATC 2 cut(s) 103, 575
Eco130I CCWWGG 1 cut(s) 483
Eco47I GGWCC 2 cut(s) 118, 389
Eco57I CTGAAG 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 184, 568
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 4 cut(s) 238, 310, 415, 487
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FaqI GGGAC 2 cut(s) 105, 200
FatI CATG 4 cut(s) 234, 306, 411, 483
FauI CCCGC 1 cut(s) 574
Fnu4HI GCNGC 2 cut(s) 40, 43
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 2 cut(s) 40, 43
FspBI CTAG 2 cut(s) 207, 254
GlaI GCGC 1 cut(s) 15
GluI GCNGC 2 cut(s) 40, 43
GsuI CTGGAG 1 cut(s) 179
HapII CCGG 1 cut(s) 144
HgaI GACGC 1 cut(s) 34
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 4 cut(s) 238, 310, 415, 487
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HinfI GANTC 4 cut(s) 53, 99, 110, 397
HpaII CCGG 1 cut(s) 144
Hpy166II GTNNAC 1 cut(s) 438
Hpy188I TCNGA 1 cut(s) 324
Hpy188III TCNNGA 3 cut(s) 107, 144, 222
Hpy8I GTNNAC 1 cut(s) 438
Hpy99I CGWCG 4 cut(s) 50, 70, 73, 76
HpyAV CCTTC 4 cut(s) 88, 228, 308, 382
HpyCH4III ACNGT 2 cut(s) 139, 442
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 2 cut(s) 4, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 551
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 1 cut(s) 293
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 4 cut(s) 238, 310, 415, 487
HspAI GCGC 1 cut(s) 14
Kpn2I TCCGGA 1 cut(s) 143
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 2 cut(s) 103, 575
LmnI GCTCC 2 cut(s) 189, 495
Lsp1109I GCAGC 1 cut(s) 51
LweI GCATC 3 cut(s) 306, 377, 608
MaeI CTAG 2 cut(s) 207, 254
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 1 cut(s) 525
MalI GATC 2 cut(s) 105, 577
MboI GATC 2 cut(s) 103, 575
MboII GAAGA 3 cut(s) 408, 508, 597
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 1 cut(s) 496
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 3 cut(s) 94, 203, 330
MroI TCCGGA 1 cut(s) 143
MseI TTAA 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 2 cut(s) 186, 570
MvaI CCWGG 2 cut(s) 186, 570
MwoI GCNNNNNNNGC 1 cut(s) 551
NcoI CCATGG 1 cut(s) 483
NdeII GATC 2 cut(s) 103, 575
NlaIII CATG 4 cut(s) 238, 310, 415, 487
NlaIV GGNNCC 2 cut(s) 191, 469
NspI RCATGY 1 cut(s) 415
PciI ACATGT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PfeI GAWTC 3 cut(s) 99, 110, 397
PkrI GCNGC 2 cut(s) 41, 44
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PscI ACATGT 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 184, 568
PspGI CCWGG 2 cut(s) 184, 568
PspN4I GGNNCC 2 cut(s) 191, 469
PspPI GGNCC 2 cut(s) 118, 389
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 1 cut(s) 575
RsaI GTAC 2 cut(s) 469, 563
RsaNI GTAC 2 cut(s) 468, 562
Rsr2I CGGWCCG 1 cut(s) 118
RsrII CGGWCCG 1 cut(s) 118
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 2 cut(s) 40, 43
Sau3AI GATC 2 cut(s) 103, 575
Sau96I GGNCC 2 cut(s) 118, 389
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 186, 570
SduI GDGCHC 1 cut(s) 492
SetI ASST 6 cut(s) 187, 232, 296, 391, 405, 534
SfaNI GCATC 3 cut(s) 306, 377, 608
SinI GGWCC 2 cut(s) 118, 389
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 1 cut(s) 496
SsiI CCGC 3 cut(s) 42, 116, 581
SspMI CTAG 2 cut(s) 207, 254
StyD4I CCNGG 2 cut(s) 184, 568
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 139, 442
TaiI ACGT 1 cut(s) 296
TaqI TCGA 3 cut(s) 48, 102, 108
TasI AATT 1 cut(s) 496
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 3 cut(s) 99, 110, 397
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 1 cut(s) 177
TseI GCWGC 1 cut(s) 39
TspGWI ACGGA 3 cut(s) 110, 423, 598
TspRI CASTG 1 cut(s) 177
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 118, 389
XceI RCATGY 1 cut(s) 415
XspI CTAG 2 cut(s) 207, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.