AT4G27250

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013441
Physical Location & Seq
Reverse (-)
13642515 .. 13644507
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27250.2

Sequence Viewer

Length: 1065 bp
ATGGAATTGCAAGGAGAGGAAAGCAAAACGGCGACGTATTGCGTGACGGGTGCAAGTGGATACATCGGTTCTTGGTTGGTTAAGTCTCTTCTCCAAAGAGGTTACACAGTCCATGCCACTCTCAGAGATCTTGCAAAATCTGAATACTTTCAATCTAAATGGAAGGAAAATGAACGGCTCAGATTATTCCGAGCGGATCTTCGAGATGACGGCAGCTTTGACGACGCCGTTAAGGGTTGTGACGGCGTCTTCCATGTCGCCGCTTCCATGGAGTTTGATATCTCATCAGATCACGTTAATCTCGAGAGTTATGTCCAGAGCAAAGTCATAGAACCGGCGCTAAAAGGCGTACGGAATGTACTTAGTTCTTGTCTAAAGTCAAAGTCAGTCAAAAGAGTTGTCTTCACATCATCCATCAGCACTCTCACTGCTAAAGATGAGAATGAACGGATGAGATCTTTCGTGGATGAAACATGCAAGGCTCATGTTGATCATGTCCTTAAAACACAAGCAAGTGGATGGATTTATGTATTATCGAAGCTCGTATCAGAGGAAGAAGCATTTCGGTATGCGAAAGAGAGAGGAATGGATCTTGTTTCAGTCATTACAACAACTGTTTCAGGTCCATTCCTTACTCCATTTGTCCCATCAAGCGTTCAAGTTCTCTTGTCTCCAATTACAGGTGACTCAAAGTTGTTTGCGATATTATCAGCTGTGAACAAAAGAATGGGTTCGATTGCTTTAGTACACATTGAAGATATTTGCAGGGCTCATCTGTTTTTGATGGAACAACCAAAGGCTAAAGGTCAATATATATGTTGTGTTGACAATATTGATATGCATGAGTTGATGCTTCATCACTTCTCTAAGGACTATCTTTGTAAAGTTCAAAAGGTAAATGAAGACGAGGAAGAGAGAGAATGTATGAAGCCTATAATTTCTTCAAAGAAGTTAAGAGAATTGGGGTTTGAGTACAAATATGGTATTGAAGAAATTGTTGATCAAACCATTGATGCTTCTATCAAGATTAAGTTCCCTACCTTGAACCACAAACTCAGGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

354

Amino Acids

40.06

Weight (kDa)

6.61

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 11 - 144 3.1e-06 KR domain
NmrA PF05368 12 - 98 6.2e-12 NmrA-like family
Epimerase PF01370 14 - 259 4.3e-28 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 14 - 138 5.5e-06 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 15 - 323 2.8e-15 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 15 - 152 4.7e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 15 - 199 8.8e-10 Male sterility protein
NAD_binding_10 PF13460 17 - 188 1.4e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 194
AciI CCGC 2 cut(s) 194, 261
AclWI GGATC 2 cut(s) 204, 597
AcyI GRCGYC 2 cut(s) 225, 246
AfaI GTAC 4 cut(s) 351, 360, 747, 974
AfiI CCNNNNNNNGG 1 cut(s) 680
AgsI TTSAA 7 cut(s) 152, 659, 755, 890, 945, 989, 1045
AluBI AGCT 3 cut(s) 216, 541, 713
AluI AGCT 3 cut(s) 216, 541, 713
Alw26I GTCTC 2 cut(s) 90, 675
AlwI GGATC 2 cut(s) 204, 597
Ama87I CYCGRG 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 213
ArsI GACNNNNNNTTYG 4 cut(s) 200, 232, 384, 416
Asp700I GAANNNNTTC 3 cut(s) 147, 561, 730
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 1 cut(s) 623
AsuHPI GGTGA 1 cut(s) 695
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 623
BaeI ACNNNNGTAYC 2 cut(s) 52, 85
BanII GRGCYC 1 cut(s) 772
BbsI GAAGAC 3 cut(s) 241, 394, 909
BbvI GCAGC 1 cut(s) 225
BccI CCATC 4 cut(s) 422, 513, 655, 778
BceAI ACGGC 5 cut(s) 45, 191, 212, 226, 259
BciVI GTATCC 1 cut(s) 53
BclI TGATCA 2 cut(s) 490, 1000
BcoDI GTCTC 2 cut(s) 90, 675
BfoI RGCGCY 1 cut(s) 341
BfuI GTATCC 1 cut(s) 53
BglII AGATCT 2 cut(s) 127, 455
BisI GCNGC 2 cut(s) 214, 261
BlsI GCNGC 2 cut(s) 215, 262
Bme18I GGWCC 1 cut(s) 623
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 623
BmsI GCATC 2 cut(s) 840, 1003
BpiI GAAGAC 3 cut(s) 241, 394, 909
BsaHI GRCGYC 2 cut(s) 225, 246
BsaJI CCNNGG 1 cut(s) 267
Bsc4I CCNNNNNNNGG 1 cut(s) 680
Bse118I RCCGGY 1 cut(s) 334
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 4 cut(s) 410, 456, 472, 524
BseLI CCNNNNNNNGG 1 cut(s) 680
BseMII CTCAG 2 cut(s) 136, 193
BseXI GCAGC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 1 cut(s) 335
BsiWI CGTACG 1 cut(s) 349
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 1 cut(s) 680
BsmAI GTCTC 2 cut(s) 90, 675
BsmFI GGGAC 1 cut(s) 629
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 772
Bsp143I GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 2 cut(s) 194, 261
BspCNI CTCAG 2 cut(s) 135, 192
BspPI GGATC 2 cut(s) 204, 597
BsrBI CCGCTC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
BssNI GRCGYC 2 cut(s) 225, 246
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 109, 616
Bst6I CTCTTC 2 cut(s) 93, 906
BstACI GRCGYC 2 cut(s) 225, 246
BstDEI CTNAG 5 cut(s) 122, 179, 362, 867, 1055
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 4 cut(s) 410, 456, 472, 524
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 7 cut(s) 130, 199, 292, 458, 493, 592, 1003
BstMAI GTCTC 2 cut(s) 90, 675
BstMBI GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
BstNSI RCATGY 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 225
BstV2I GAAGAC 3 cut(s) 241, 394, 909
BstX2I RGATCY 4 cut(s) 127, 196, 455, 589
BstYI RGATCY 4 cut(s) 127, 196, 455, 589
BsuI GTATCC 1 cut(s) 53
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 4 cut(s) 410, 456, 472, 524
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 623
CseI GACGC 2 cut(s) 233, 235
Csp6I GTAC 4 cut(s) 350, 359, 746, 973
CviAII CATG 7 cut(s) 113, 254, 268, 474, 485, 494, 842
CviJI RGCY 8 cut(s) 178, 216, 482, 541, 713, 770, 800, 931
CviKI_1 RGCY 8 cut(s) 178, 216, 482, 541, 713, 770, 800, 931
CviQI GTAC 4 cut(s) 350, 359, 746, 973
DdeI CTNAG 5 cut(s) 122, 179, 362, 867, 1055
DpnI GATC 7 cut(s) 129, 198, 291, 457, 492, 591, 1002
DpnII GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
Eam1104I CTCTTC 2 cut(s) 93, 906
EarI CTCTTC 2 cut(s) 93, 906
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 772
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 302
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 267
EcoT22I ATGCAT 1 cut(s) 843
EcoT38I GRGCYC 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 7 cut(s) 116, 257, 271, 477, 488, 497, 845
FaqI GGGAC 1 cut(s) 629
FatI CATG 7 cut(s) 112, 253, 267, 473, 484, 493, 841
FbaI TGATCA 2 cut(s) 490, 1000
Fnu4HI GCNGC 2 cut(s) 214, 261
FokI GGATG 4 cut(s) 397, 463, 479, 531
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 2 cut(s) 214, 261
GlaI GCGC 1 cut(s) 339
GluI GCNGC 2 cut(s) 214, 261
HaeII RGCGCY 1 cut(s) 341
HapII CCGG 1 cut(s) 335
HgaI GACGC 2 cut(s) 233, 235
HhaI GCGC 1 cut(s) 340
Hin1I GRCGYC 2 cut(s) 225, 246
Hin1II CATG 7 cut(s) 116, 257, 271, 477, 488, 497, 845
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 1 cut(s) 686
HpaII CCGG 1 cut(s) 335
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 3 cut(s) 718, 748, 826
Hpy188I TCNGA 6 cut(s) 125, 142, 182, 191, 289, 550
Hpy188III TCNNGA 5 cut(s) 203, 302, 304, 316, 1024
Hpy8I GTNNAC 3 cut(s) 718, 748, 826
Hpy99I CGWCG 2 cut(s) 37, 227
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 2 cut(s) 109, 616
HpyCH4IV ACGT 2 cut(s) 35, 294
HpyCH4V TGCA 6 cut(s) 10, 53, 134, 477, 765, 841
HpyF3I CTNAG 5 cut(s) 122, 179, 362, 867, 1055
HpySE526I ACGT 2 cut(s) 35, 294
Hsp92I GRCGYC 2 cut(s) 225, 246
Hsp92II CATG 7 cut(s) 116, 257, 271, 477, 488, 497, 845
HspAI GCGC 1 cut(s) 338
Ksp22I TGATCA 2 cut(s) 490, 1000
Kzo9I GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
LpnPI CCDG 6 cut(s) 329, 348, 606, 666, 751, 1042
Lsp1109I GCAGC 1 cut(s) 225
LweI GCATC 2 cut(s) 840, 1003
MaeII ACGT 2 cut(s) 35, 294
MaeIII GTNAC 4 cut(s) 43, 101, 239, 683
MalI GATC 7 cut(s) 129, 198, 291, 457, 492, 591, 1002
MbiI CCGCTC 1 cut(s) 194
MboI GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
MflI RGATCY 4 cut(s) 127, 196, 455, 589
MhlI GDGCHC 1 cut(s) 772
MluCI AATT 5 cut(s) 5, 675, 936, 959, 993
MlyI GAGTC 1 cut(s) 680
MnlI CCTC 5 cut(s) 10, 92, 544, 575, 901
Mph1103I ATGCAT 1 cut(s) 843
MroXI GAANNNNTTC 3 cut(s) 147, 561, 730
MseI TTAA 6 cut(s) 81, 231, 297, 501, 953, 1029
MspA1I CMGCKG 1 cut(s) 713
MspI CCGG 1 cut(s) 335
NcoI CCATGG 1 cut(s) 267
NdeII GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
NlaIII CATG 7 cut(s) 116, 257, 271, 477, 488, 497, 845
NmuCI GTSAC 3 cut(s) 43, 239, 683
NsiI ATGCAT 1 cut(s) 843
NspI RCATGY 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PdmI GAANNNNTTC 3 cut(s) 147, 561, 730
Pfl23II CGTACG 1 cut(s) 349
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 2 cut(s) 215, 262
PleI GAGTC 1 cut(s) 680
PpsI GAGTC 1 cut(s) 680
PspLI CGTACG 1 cut(s) 349
PspPI GGNCC 1 cut(s) 623
PsuI RGATCY 4 cut(s) 127, 196, 455, 589
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 713
RsaI GTAC 4 cut(s) 351, 360, 747, 974
RsaNI GTAC 4 cut(s) 350, 359, 746, 973
SaqAI TTAA 6 cut(s) 81, 231, 297, 501, 953, 1029
SatI GCNGC 2 cut(s) 214, 261
Sau3AI GATC 7 cut(s) 127, 196, 289, 455, 490, 589, 1000
Sau96I GGNCC 1 cut(s) 623
SchI GAGTC 1 cut(s) 680
SduI GDGCHC 1 cut(s) 772
SfaNI GCATC 2 cut(s) 840, 1003
Sfr274I CTCGAG 1 cut(s) 302
SinI GGWCC 1 cut(s) 623
SlaI CTCGAG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 5 cut(s) 5, 675, 936, 959, 993
SsiI CCGC 2 cut(s) 194, 261
SspI AATATT 1 cut(s) 832
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 109, 616
TaiI ACGT 2 cut(s) 38, 297
TaqI TCGA 4 cut(s) 202, 303, 536, 734
TasI AATT 5 cut(s) 5, 675, 936, 959, 993
TatI WGTACW 3 cut(s) 358, 745, 972
TauI GCSGC 1 cut(s) 263
Tru1I TTAA 6 cut(s) 81, 231, 297, 501, 953, 1029
Tru9I TTAA 6 cut(s) 81, 231, 297, 501, 953, 1029
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 3 cut(s) 43, 239, 683
TseI GCWGC 1 cut(s) 213
Tsp45I GTSAC 3 cut(s) 43, 239, 683
TspDTI ATGAA 6 cut(s) 186, 459, 483, 845, 915, 941
TspGWI ACGGA 2 cut(s) 367, 463
TspRI CASTG 1 cut(s) 433
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 623
XceI RCATGY 1 cut(s) 477
XhoI CTCGAG 1 cut(s) 302
XmnI GAANNNNTTC 3 cut(s) 147, 561, 730
Zsp2I ATGCAT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.