MD11G1229100.v1.1

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013441
Physical Location & Seq
Reverse (-)
33307428 .. 33310083
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1229100.v1.1.491

Sequence Viewer

Length: 783 bp
ATGGAATTCAGTGTTACCGTAACAGAAAACATTGAGAGCTATGTTCAATCAAATACCATTGAGCCTGCAATCAAAGGAACCATAGACCTCCTCCAATCCTGTGTGAATTCTGGAACGGTGAAAAGGGTTGTACTCACCTCTTCCATTAGTACCATCATTGCCAAAGATGGCAACGGAAATGCAAGAGCTGTTGTTGATGAGTCTTGCCAAACTCCAGTTGATCAAGTTCTAAGTGCAAAAGCAAGTGGATGGGTTTATGTACTTTCCAAGATTCTCACAGAGGAAGCAGCATTCAGATTTGCAAGTGATAATGGGATTAATCTTGTATCAGTCATAACAAGTACTGTTGGGGGCAATTTTCTCACTTCCAAGGTTCCATCAAGCATTCAAGTTCTCTTATCTCCAATAACTGGTGACTCTGAACTTTATGGCATCTTATCTGCGGTAAATGCAAGGATGGGATCAATTGCTTTAGTTCATGTTGAAGATATATGCAATGCCCACATCTTTTTAATGGAGCACACCAAAGCGGAAGGTCGTTACATATGCGTTGCCCAAAGTTGTCCAATATCTAAGTTGCTTAACTATCTAGCTCAGGCGTATCCTTGCTCAAATTCAAACAGGTACAGAGTGATTGAGGAAGAAAATGATGTAGTACCCTATTCCGAGATCTCTTCAAAGAAGCTAACAGATTTGGGATTCACTTACAAGCATGGAATTGAAGATATGGTTCACCAGACAGTTTCTTGTTGTGTAGATTATGGCTTTCTACCTCCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

261

Amino Acids

28.16

Weight (kDa)

5.02

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 17 - 177 1e-08 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 410
AciI CCGC 2 cut(s) 443, 530
AclWI GGATC 1 cut(s) 469
AcsI RAATTY 3 cut(s) 5, 106, 613
AfaI GTAC 6 cut(s) 132, 151, 261, 343, 626, 657
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 6 cut(s) 47, 389, 485, 618, 678, 722
AluBI AGCT 4 cut(s) 39, 188, 593, 685
AluI AGCT 4 cut(s) 39, 188, 593, 685
Alw21I GWGCWC 1 cut(s) 522
AlwI GGATC 1 cut(s) 469
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 3 cut(s) 5, 106, 613
AseI ATTAAT 1 cut(s) 318
AsuHPI GGTGA 4 cut(s) 127, 130, 425, 725
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 1 cut(s) 299
BccI CCATC 5 cut(s) 161, 161, 243, 385, 451
BciVI GTATCC 1 cut(s) 612
BclI TGATCA 1 cut(s) 220
BfaI CTAG 1 cut(s) 590
BfuI GTATCC 1 cut(s) 612
BglII AGATCT 1 cut(s) 669
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
BmcAI AGTACT 1 cut(s) 343
BmiI GGNNCC 2 cut(s) 79, 375
BmsI GCATC 1 cut(s) 441
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 594
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 2 cut(s) 215, 415
Bse3DI GCAATG 2 cut(s) 156, 502
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 254, 462
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMI GCAATG 2 cut(s) 156, 502
BseMII CTCAG 1 cut(s) 608
BseNI ACTGG 2 cut(s) 215, 415
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 522
BslI CCNNNNNNNGG 1 cut(s) 410
BsmI GAATGC 2 cut(s) 290, 384
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 3 cut(s) 220, 461, 669
BspACI CCGC 2 cut(s) 443, 530
BspCNI CTCAG 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 79, 375
BspPI GGATC 1 cut(s) 469
BsrDI GCAATG 2 cut(s) 156, 502
BsrI ACTGG 2 cut(s) 215, 415
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 3 cut(s) 220, 461, 669
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 4 cut(s) 19, 118, 346, 742
Bst6I CTCTTC 2 cut(s) 145, 679
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 3 cut(s) 230, 573, 594
BstF5I GGATG 2 cut(s) 254, 462
BstKTI GATC 3 cut(s) 223, 464, 672
BstMBI GATC 3 cut(s) 220, 461, 669
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstV1I GCAGC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 669
BstYI RGATCY 1 cut(s) 669
BsuI GTATCC 1 cut(s) 612
BtsCI GGATG 2 cut(s) 254, 462
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 66
Csp6I GTAC 6 cut(s) 131, 150, 260, 342, 625, 656
CviAII CATG 2 cut(s) 479, 713
CviJI RGCY 6 cut(s) 39, 64, 188, 593, 685, 765
CviKI_1 RGCY 6 cut(s) 39, 64, 188, 593, 685, 765
CviQI GTAC 6 cut(s) 131, 150, 260, 342, 625, 656
DdeI CTNAG 3 cut(s) 230, 573, 594
DpnI GATC 3 cut(s) 222, 463, 671
DpnII GATC 3 cut(s) 220, 461, 669
Eam1104I CTCTTC 2 cut(s) 145, 679
EarI CTCTTC 2 cut(s) 145, 679
Eco130I CCWWGG 1 cut(s) 369
EcoRI GAATTC 2 cut(s) 5, 106
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 2 cut(s) 482, 716
FatI CATG 2 cut(s) 478, 712
FauNDI CATATG 1 cut(s) 545
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 2 cut(s) 261, 469
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 590
GluI GCNGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 198
Hin1II CATG 2 cut(s) 482, 716
HinfI GANTC 4 cut(s) 200, 271, 416, 699
HphI GGTGA 4 cut(s) 127, 130, 425, 725
Hpy166II GTNNAC 1 cut(s) 733
Hpy188I TCNGA 3 cut(s) 296, 421, 667
Hpy188III TCNNGA 1 cut(s) 111
Hpy8I GTNNAC 1 cut(s) 733
HpyAV CCTTC 1 cut(s) 527
HpyCH4III ACNGT 4 cut(s) 19, 118, 346, 742
HpyCH4V TGCA 6 cut(s) 68, 182, 236, 302, 452, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 3 cut(s) 230, 573, 594
Hsp92II CATG 2 cut(s) 482, 716
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 220, 461, 669
LmnI GCTCC 1 cut(s) 517
LpnPI CCDG 8 cut(s) 78, 96, 112, 228, 396, 581, 607, 749
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 1 cut(s) 441
MaeI CTAG 1 cut(s) 590
MaeIII GTNAC 4 cut(s) 13, 19, 413, 539
MalI GATC 3 cut(s) 222, 463, 671
MboI GATC 3 cut(s) 220, 461, 669
MboII GAAGA 5 cut(s) 132, 497, 653, 666, 734
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 6 cut(s) 5, 106, 355, 465, 613, 717
MlyI GAGTC 2 cut(s) 209, 410
MnlI CCTC 6 cut(s) 98, 101, 148, 274, 631, 783
MseI TTAA 3 cut(s) 318, 512, 582
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 2 cut(s) 290, 384
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeI CATATG 1 cut(s) 545
NdeII GATC 3 cut(s) 220, 461, 669
NlaIII CATG 2 cut(s) 482, 716
NlaIV GGNNCC 2 cut(s) 79, 375
NmuCI GTSAC 1 cut(s) 413
PctI GAATGC 2 cut(s) 290, 384
PfeI GAWTC 2 cut(s) 271, 699
PflMI CCANNNNNTGG 1 cut(s) 410
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 2 cut(s) 208, 410
PpsI GAGTC 2 cut(s) 208, 410
PshBI ATTAAT 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 79, 375
PsuI RGATCY 1 cut(s) 669
RsaI GTAC 6 cut(s) 132, 151, 261, 343, 626, 657
RsaNI GTAC 6 cut(s) 131, 150, 260, 342, 625, 656
SaqAI TTAA 3 cut(s) 318, 512, 582
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 3 cut(s) 220, 461, 669
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 2 cut(s) 209, 410
SduI GDGCHC 1 cut(s) 522
SfaNI GCATC 1 cut(s) 441
Sse9I AATT 6 cut(s) 5, 106, 355, 465, 613, 717
SsiI CCGC 2 cut(s) 443, 530
SspMI CTAG 1 cut(s) 590
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 19, 118, 346, 742
TasI AATT 6 cut(s) 5, 106, 355, 465, 613, 717
TatI WGTACW 3 cut(s) 130, 259, 341
TfiI GAWTC 2 cut(s) 271, 699
Tru1I TTAA 3 cut(s) 318, 512, 582
Tru9I TTAA 3 cut(s) 318, 512, 582
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 467
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 1 cut(s) 16
Van91I CCANNNNNTGG 1 cut(s) 410
VspI ATTAAT 1 cut(s) 318
XapI RAATTY 3 cut(s) 5, 106, 613
XspI CTAG 1 cut(s) 590
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.