Rroxscaffold_1G00043880

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013441
Physical Location & Seq
Reverse (-)
62560405 .. 62562014
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043880.1

Sequence Viewer

Length: 903 bp
ATGCCAATATACCGATTATTCTACAGATTATTCCGAGCTGATTTGCAAGAAGAAGGAAGCTTCGACGAGGCTGTTAAGGGATGCGATGGCGTGTTTCACGTTGCAGCATCAATGCAATTCAGTGTTCCTGTGGAAGAGAACATTGATGCTTATGTTCAATCAAATATCATCGACCCTGCAATCAAAGGAACCTTGGACCTCCTCAAATCCTGTGTGAAATCGGGTACCGTGAATACGGTTGTGCTTACCTCTTCTATCAGTACTATGACTGCCAAAGATAGCAGTGGAAATCCAATCTCTGTTGTTGACGAATCTTGCAAAACTCCAGTTGATCGTGTCTGGGATGCGAAAGCAAGTGGATGGGTTTATGCTCTTTCAAAGATCCTCACAGAGGAAGCAGCATTTAGATTTGCAAGTGAGAATGGGATTAAGCTTGTTTCAGTAATAACAAGTAATGTTGGAGGACCATTTCTCACTTCCAATGTTCCATCAAGCATTCGAGTTCTTTTGTCACCATTGACAGGTGACTCTGAGCTCTATGGAATATTAACTGCTGTAAATGCAAGAATGGGATCAATTGTATTAGTTCACATTGAGGACATATGCAATGCCCATATTTTTTTAATGGAGAATGATGAAGCAGAAGGAAGATACATATGTGCTGCCAAAAGTATTCCAATGTCTGAGTTGGTTCTTTATCTAGCTCAAGCATTTCCTTCCTCAAATTCACAGAGGGTTGTGGTGGAGGAAGAACAGAATAATCTAGCACCCTCTGAGATATCTTCAAAGAAGCTAAGTGATTTAGGTTTTAATTACAAGCATGGAGTAGACGATATTATTCACCAGTCAGTTTCTTCTTGTCTGGATTTTGGCCTTCTACCTCATACTGGAAATTGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

300

Amino Acids

32.51

Weight (kDa)

4.81

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 9 - 214 6.2e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 12 - 133 8.3e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 224
AccI GTMKAC 1 cut(s) 828
AclWI GGATC 2 cut(s) 376, 580
AcsI RAATTY 1 cut(s) 724
AfaI GTAC 2 cut(s) 226, 262
AfiI CCNNNNNNNGG 3 cut(s) 391, 521, 887
AgsI TTSAA 3 cut(s) 158, 378, 786
AluBI AGCT 6 cut(s) 38, 60, 433, 535, 704, 793
AluI AGCT 6 cut(s) 38, 60, 433, 535, 704, 793
Alw21I GWGCWC 1 cut(s) 537
AlwI GGATC 2 cut(s) 376, 580
AoxI GGCC 1 cut(s) 871
ApeKI GCWGC 3 cut(s) 104, 398, 662
ApoI RAATTY 1 cut(s) 724
Asp718I GGTACC 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 196, 464
AsuHPI GGTGA 3 cut(s) 504, 536, 833
AvaII GGWCC 2 cut(s) 196, 464
BaeI ACNNNNGTAYC 2 cut(s) 643, 676
BanI GGYRCC 1 cut(s) 224
BanII GRGCYC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 3 cut(s) 116, 410, 649
BccI CCATC 3 cut(s) 80, 354, 496
BfaI CTAG 3 cut(s) 701, 764, 901
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 3 cut(s) 105, 399, 663
BlsI GCNGC 3 cut(s) 106, 400, 664
BmcAI AGTACT 1 cut(s) 262
Bme18I GGWCC 2 cut(s) 196, 464
BmgT120I GGNCC 2 cut(s) 196, 464
BmiI GGNNCC 2 cut(s) 190, 226
BmsI GCATC 4 cut(s) 71, 116, 136, 334
BpmI CTGGAG 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 690
BsaJI CCNNGG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 521, 887
Bse1I ACTGG 3 cut(s) 326, 844, 892
Bse3DI GCAATG 1 cut(s) 613
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 3 cut(s) 86, 349, 365
BseLI CCNNNNNNNGG 3 cut(s) 391, 521, 887
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 3 cut(s) 522, 675, 765
BseNI ACTGG 3 cut(s) 326, 844, 892
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 3 cut(s) 116, 410, 649
BshFI GGCC 1 cut(s) 873
BshNI GGYRCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 537
BslI CCNNNNNNNGG 3 cut(s) 391, 521, 887
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 1 cut(s) 873
Bsp1286I GDGCHC 1 cut(s) 537
Bsp143I GATC 3 cut(s) 331, 381, 572
BspANI GGCC 1 cut(s) 873
BspCNI CTCAG 3 cut(s) 523, 676, 766
BspLI GGNNCC 2 cut(s) 190, 226
BspPI GGATC 2 cut(s) 376, 580
BspT107I GGYRCC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 3 cut(s) 326, 844, 892
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 3 cut(s) 331, 381, 572
BssT1I CCWWGG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 229, 238
Bst6I CTCTTC 2 cut(s) 129, 256
BstDEI CTNAG 4 cut(s) 531, 684, 774, 794
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 3 cut(s) 86, 349, 365
BstKTI GATC 3 cut(s) 334, 384, 575
BstMBI GATC 3 cut(s) 331, 381, 572
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstSFI CTRYAG 1 cut(s) 22
BstV1I GCAGC 3 cut(s) 116, 410, 649
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BsuRI GGCC 1 cut(s) 873
BtgZI GCGATG 1 cut(s) 99
BtsCI GGATG 3 cut(s) 86, 349, 365
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 2 cut(s) 127, 289
Cfr13I GGNCC 2 cut(s) 196, 464
Csp6I GTAC 2 cut(s) 225, 261
CviAII CATG 1 cut(s) 821
CviJI RGCY 8 cut(s) 38, 60, 71, 433, 535, 704, 793, 873
CviKI_1 RGCY 8 cut(s) 38, 60, 71, 433, 535, 704, 793, 873
CviQI GTAC 2 cut(s) 225, 261
DdeI CTNAG 4 cut(s) 531, 684, 774, 794
DpnI GATC 3 cut(s) 333, 383, 574
DpnII GATC 3 cut(s) 331, 381, 572
Eam1104I CTCTTC 2 cut(s) 129, 256
EarI CTCTTC 2 cut(s) 129, 256
Ecl136II GAGCTC 1 cut(s) 535
Eco130I CCWWGG 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 537
Eco32I GATATC 1 cut(s) 780
Eco47I GGWCC 2 cut(s) 196, 464
Eco53kI GAGCTC 1 cut(s) 535
EcoICRI GAGCTC 1 cut(s) 535
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoRV GATATC 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 1 cut(s) 824
FatI CATG 1 cut(s) 820
FauNDI CATATG 2 cut(s) 602, 656
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 3 cut(s) 105, 399, 663
FokI GGATG 3 cut(s) 93, 356, 372
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 3 cut(s) 105, 399, 663
FspBI CTAG 3 cut(s) 701, 764, 901
GluI GCNGC 3 cut(s) 105, 399, 663
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 873
Hin1II CATG 1 cut(s) 824
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 2 cut(s) 58, 431
HinfI GANTC 2 cut(s) 311, 527
HphI GGTGA 3 cut(s) 504, 536, 833
Hpy166II GTNNAC 3 cut(s) 307, 589, 829
Hpy188I TCNGA 4 cut(s) 35, 532, 685, 775
Hpy188III TCNNGA 1 cut(s) 863
Hpy8I GTNNAC 3 cut(s) 307, 589, 829
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 4 cut(s) 47, 638, 726, 884
HpyCH4III ACNGT 2 cut(s) 229, 238
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 8 cut(s) 46, 104, 115, 179, 318, 413, 563, 606
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 4 cut(s) 531, 684, 774, 794
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 1 cut(s) 824
KpnI GGTACC 1 cut(s) 228
Kzo9I GATC 3 cut(s) 331, 381, 572
LpnPI CCDG 9 cut(s) 141, 189, 223, 325, 339, 507, 848, 857, 873
Lsp1109I GCAGC 3 cut(s) 116, 410, 649
LweI GCATC 4 cut(s) 71, 116, 136, 334
MaeI CTAG 3 cut(s) 701, 764, 901
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 3 cut(s) 510, 524, 896
MalI GATC 3 cut(s) 333, 383, 574
MboI GATC 3 cut(s) 331, 381, 572
MboII GAAGA 7 cut(s) 62, 146, 243, 660, 761, 774, 846
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 5 cut(s) 116, 576, 724, 811, 892
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 439
MseI TTAA 5 cut(s) 75, 429, 548, 623, 810
MunI CAATTG 1 cut(s) 576
Mva1269I GAATGC 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 560
NdeI CATATG 2 cut(s) 602, 656
NdeII GATC 3 cut(s) 331, 381, 572
NlaIII CATG 1 cut(s) 824
NlaIV GGNNCC 2 cut(s) 190, 226
NmuCI GTSAC 2 cut(s) 510, 524
PctI GAATGC 1 cut(s) 495
PfeI GAWTC 1 cut(s) 311
PkrI GCNGC 3 cut(s) 106, 400, 664
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Psp124BI GAGCTC 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 190, 226
PspPI GGNCC 2 cut(s) 196, 464
PsuI RGATCY 1 cut(s) 381
RsaI GTAC 2 cut(s) 226, 262
RsaNI GTAC 2 cut(s) 225, 261
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 5 cut(s) 75, 429, 548, 623, 810
SatI GCNGC 3 cut(s) 105, 399, 663
Sau3AI GATC 3 cut(s) 331, 381, 572
Sau96I GGNCC 2 cut(s) 196, 464
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 1 cut(s) 521
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 4 cut(s) 71, 116, 136, 334
SfcI CTRYAG 1 cut(s) 22
SinI GGWCC 2 cut(s) 196, 464
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
Sse9I AATT 5 cut(s) 116, 576, 724, 811, 892
SspI AATATT 1 cut(s) 546
SspMI CTAG 3 cut(s) 701, 764, 901
SstI GAGCTC 1 cut(s) 537
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 229, 238
TaiI ACGT 1 cut(s) 102
TaqI TCGA 3 cut(s) 63, 171, 499
TasI AATT 5 cut(s) 116, 576, 724, 811, 892
TatI WGTACW 1 cut(s) 260
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 5 cut(s) 75, 429, 548, 623, 810
Tru9I TTAA 5 cut(s) 75, 429, 548, 623, 810
TscAI CASTG 2 cut(s) 127, 289
TseFI GTSAC 2 cut(s) 510, 524
TseI GCWGC 3 cut(s) 104, 398, 662
Tsp45I GTSAC 2 cut(s) 510, 524
TspDTI ATGAA 1 cut(s) 651
TspRI CASTG 2 cut(s) 127, 289
VpaK11BI GGWCC 2 cut(s) 196, 464
XagI CCTNNNNNAGG 1 cut(s) 389
XapI RAATTY 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 3 cut(s) 701, 764, 901
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.