MD03G1214100.v1.1

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013441
Physical Location & Seq
Reverse (-)
29380661 .. 29386685
6025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1214100.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGCATATCTCTCTGTGTGTTTATATATATTTCTGTGTGTGTGTGTCAGCGTGCGGGCAAAAGAAAGGGAGAGCTATGGCAACTTACTGTGTTACCGGAGCCACTGGTTTTATAGGGTCTTGGTTGGTGAAGAGCCTCCTGGAGAAAGGCCACATGGTTCATGCCACAGTCCGTGATCCTGCCAAGTGTTTGCACCTATTATCATTGTGGACAAGCATTGACCGGTTGAGATTGCTCCGAGCGGATTTGGGAGAAGAAGGAAGCTTCGACGAGGCTGTGAAGGGCTGCGACGGTGTATTTCACGTTGCAGCATCCATGGAATTCAGTGTTACTGTCAAAGAAAACATTGAGAGTTATGTTCGATCAAATATCATTGAGCCTGCGATCAAAGGAACCTTAGACCTCCTCAAATCCTGTGTGAATTCCGGAACGGTGAAAAGGGTTGTACTCACCTCTTCCATTAGTACTATCACCGCCAAAGACGGAAACGGAAATGCAAGAGTTGTTGTTGATGAGTCTTGCCAAACTCCAGTTGATCAAGTTCTAACTGCAAAAGCAAATGGATGGGTTTATGTACTTTTGAAGATTCTCACGGAGGAAGCAGCATTCAAATTTGCAAGTGAGAAAGGGATTAATCTTGTATCAGTCATAACAAGTACTGTTGGGGGGCCTTTTCTCACTTCCAAAGTTCCATCAAGCATTCAAGTTCTTCTTTCTCCAGTAACAGGTGACTCTGAACTCTATGGCATATTATCTGCTGTAAATGCAAGGATGGGATCAATTGCGTTAGTTCACATTGAAGATACATGCAATGCGCACATGTTTTTAATGGAGCATGCCAAAGCTGAAGGTCGTTACGTATGCGTTGCCCAAAGTTGTCCAATATCCAAGTTGCTTAACTATCTAGCTCAGGCATATCCTTGCTCAAATTCAAACAGGTACAGAGTTATTGCGGAAGAAAATGATGTAGTACCTTCCGAGATCTCTTCAAAGAAGCTAACTGATTTGGGATTCACTTACAAGCATGGCATTGAAGATATCGTTCACCAGACAGTTTCTTGTTGTTTAGGTTATGGCTTTCTACCTCCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

365

Amino Acids

39.37

Weight (kDa)

6.78

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 27 - 165 1.5e-06 KR domain
Epimerase PF01370 30 - 283 1.4e-23 NAD dependent epimerase/dehydratase family
NmrA PF05368 30 - 107 3.3e-09 NmrA-like family
3Beta_HSD PF01073 31 - 159 1.2e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 31 - 153 4.3e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 31 - 223 3.9e-09 Male sterility protein
NAD_binding_10 PF13460 33 - 202 1.7e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 816
AccBSI CCGCTC 1 cut(s) 242
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 4 cut(s) 54, 242, 474, 953
AclWI GGATC 2 cut(s) 170, 784
AcsI RAATTY 4 cut(s) 320, 421, 611, 928
AcuI CTGAAG 1 cut(s) 867
AfaI GTAC 6 cut(s) 447, 466, 576, 658, 941, 972
AfiI CCNNNNNNNGG 1 cut(s) 725
AflIII ACRYGT 1 cut(s) 819
AgeI ACCGGT 1 cut(s) 222
AgsI TTSAA 7 cut(s) 583, 610, 704, 800, 933, 990, 1034
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 5 cut(s) 74, 264, 845, 908, 997
AluI AGCT 5 cut(s) 74, 264, 845, 908, 997
AlwI GGATC 2 cut(s) 170, 784
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 2 cut(s) 148, 668
ApeKI GCWGC 3 cut(s) 285, 308, 602
ApoI RAATTY 4 cut(s) 320, 421, 611, 928
AseI ATTAAT 1 cut(s) 633
AsiGI ACCGGT 1 cut(s) 222
AspLEI GCGC 1 cut(s) 817
AspS9I GGNCC 1 cut(s) 668
AsuHPI GGTGA 6 cut(s) 139, 442, 445, 463, 740, 1037
BbvI GCAGC 3 cut(s) 272, 320, 614
BccI CCATC 3 cut(s) 558, 700, 766
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 535
BfaI CTAG 1 cut(s) 905
BglII AGATCT 1 cut(s) 981
BisI GCNGC 3 cut(s) 286, 309, 603
BlsI GCNGC 3 cut(s) 287, 310, 604
BmcAI AGTACT 2 cut(s) 466, 658
Bme1390I CCNGG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 668
BmiI GGNNCC 3 cut(s) 100, 394, 669
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 320
BpmI CTGGAG 3 cut(s) 161, 513, 702
Bpu10I CCTNAGC 1 cut(s) 909
BsaAI YACGTR 1 cut(s) 859
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 3 cut(s) 95, 222, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 3 cut(s) 109, 530, 719
Bse3DI GCAATG 1 cut(s) 817
BseAI TCCGGA 1 cut(s) 425
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 3 cut(s) 311, 569, 777
BseLI CCNNNNNNNGG 1 cut(s) 725
BseMI GCAATG 1 cut(s) 817
BseMII CTCAG 1 cut(s) 923
BseNI ACTGG 3 cut(s) 109, 530, 719
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 3 cut(s) 272, 320, 614
BshFI GGCC 2 cut(s) 150, 670
BshTI ACCGGT 1 cut(s) 222
BsiSI CCGG 3 cut(s) 96, 223, 426
BslI CCNNNNNNNGG 1 cut(s) 725
BsmI GAATGC 2 cut(s) 605, 699
BsnI GGCC 2 cut(s) 150, 670
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 6 cut(s) 175, 362, 384, 535, 776, 981
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 4 cut(s) 54, 242, 474, 953
BspANI GGCC 2 cut(s) 150, 670
BspCNI CTCAG 1 cut(s) 922
BspEI TCCGGA 1 cut(s) 425
BspLI GGNNCC 3 cut(s) 100, 394, 669
BspPI GGATC 2 cut(s) 170, 784
BspQI GCTCTTC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 817
BsrFI RCCGGY 1 cut(s) 222
BsrI ACTGG 3 cut(s) 109, 530, 719
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 6 cut(s) 175, 362, 384, 535, 776, 981
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 7 cut(s) 89, 169, 293, 334, 433, 661, 1054
Bst6I CTCTTC 3 cut(s) 125, 460, 991
BstBAI YACGTR 1 cut(s) 859
BstC8I GCNNGC 4 cut(s) 52, 56, 381, 837
BstDEI CTNAG 2 cut(s) 397, 909
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 3 cut(s) 311, 569, 777
BstHHI GCGC 1 cut(s) 817
BstKTI GATC 6 cut(s) 178, 365, 387, 538, 779, 984
BstMBI GATC 6 cut(s) 175, 362, 384, 535, 776, 981
BstMWI GCNNNNNNNGC 1 cut(s) 764
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 3 cut(s) 810, 823, 839
BstSCI CCNGG 1 cut(s) 138
BstSNI TACGTA 1 cut(s) 859
BstV1I GCAGC 3 cut(s) 272, 320, 614
BstX2I RGATCY 1 cut(s) 981
BstYI RGATCY 1 cut(s) 981
BsuRI GGCC 2 cut(s) 150, 670
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 3 cut(s) 311, 569, 777
BtsIMutI CAGTG 2 cut(s) 102, 331
Cac8I GCNNGC 4 cut(s) 52, 56, 381, 837
CfoI GCGC 1 cut(s) 817
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 668
Csp6I GTAC 6 cut(s) 446, 465, 575, 657, 940, 971
CspAI ACCGGT 1 cut(s) 222
CviAII CATG 7 cut(s) 154, 161, 316, 807, 820, 836, 1025
CviQI GTAC 6 cut(s) 446, 465, 575, 657, 940, 971
DdeI CTNAG 2 cut(s) 397, 909
DpnI GATC 6 cut(s) 177, 364, 386, 537, 778, 983
DpnII GATC 6 cut(s) 175, 362, 384, 535, 776, 981
Eam1104I CTCTTC 3 cut(s) 125, 460, 991
EarI CTCTTC 3 cut(s) 125, 460, 991
Eco105I TACGTA 1 cut(s) 859
Eco130I CCWWGG 1 cut(s) 315
Eco32I GATATC 1 cut(s) 1039
Eco57I CTGAAG 1 cut(s) 867
EcoO109I RGGNCCY 1 cut(s) 668
EcoRI GAATTC 2 cut(s) 320, 421
EcoRII CCWGG 1 cut(s) 138
EcoRV GATATC 1 cut(s) 1039
EcoT14I CCWWGG 1 cut(s) 315
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 7 cut(s) 157, 164, 319, 810, 823, 839, 1028
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FatI CATG 7 cut(s) 153, 160, 315, 806, 819, 835, 1024
FauI CCCGC 1 cut(s) 47
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 3 cut(s) 286, 309, 603
FokI GGATG 3 cut(s) 298, 576, 784
Fsp4HI GCNGC 3 cut(s) 286, 309, 603
FspAI RTGCGCAY 1 cut(s) 816
FspBI CTAG 1 cut(s) 905
FspI TGCGCA 1 cut(s) 816
GlaI GCGC 1 cut(s) 816
GluI GCNGC 3 cut(s) 286, 309, 603
GsuI CTGGAG 3 cut(s) 161, 513, 702
HaeIII GGCC 2 cut(s) 150, 670
HapII CCGG 3 cut(s) 96, 223, 426
HhaI GCGC 1 cut(s) 817
Hin1II CATG 7 cut(s) 157, 164, 319, 810, 823, 839, 1028
Hin6I GCGC 1 cut(s) 815
HinP1I GCGC 1 cut(s) 815
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 4 cut(s) 515, 586, 731, 1011
HpaII CCGG 3 cut(s) 96, 223, 426
HphI GGTGA 6 cut(s) 139, 442, 445, 463, 740, 1037
Hpy166II GTNNAC 3 cut(s) 210, 793, 1045
Hpy188I TCNGA 3 cut(s) 239, 736, 979
Hpy188III TCNNGA 1 cut(s) 426
Hpy8I GTNNAC 3 cut(s) 210, 793, 1045
Hpy99I CGWCG 2 cut(s) 272, 293
HpyAV CCTTC 4 cut(s) 251, 274, 842, 984
HpyCH4III ACNGT 7 cut(s) 89, 169, 293, 334, 433, 661, 1054
HpyCH4IV ACGT 2 cut(s) 303, 858
HpyCH4V TGCA 8 cut(s) 4, 193, 308, 497, 551, 617, 767, 810
HpyF10VI GCNNNNNNNGC 1 cut(s) 764
HpyF3I CTNAG 2 cut(s) 397, 909
HpySE526I ACGT 2 cut(s) 303, 858
Hsp92II CATG 7 cut(s) 157, 164, 319, 810, 823, 839, 1028
HspAI GCGC 1 cut(s) 815
Kpn2I TCCGGA 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 6 cut(s) 175, 362, 384, 535, 776, 981
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 3 cut(s) 98, 240, 832
Lsp1109I GCAGC 3 cut(s) 272, 320, 614
LweI GCATC 1 cut(s) 320
MaeI CTAG 1 cut(s) 905
MaeII ACGT 2 cut(s) 303, 858
MaeIII GTNAC 5 cut(s) 91, 328, 721, 728, 854
MalI GATC 6 cut(s) 177, 364, 386, 537, 778, 983
MbiI CCGCTC 1 cut(s) 242
MboI GATC 6 cut(s) 175, 362, 384, 535, 776, 981
MboII GAAGA 9 cut(s) 142, 266, 447, 595, 701, 812, 968, 978, 1046
MfeI CAATTG 1 cut(s) 780
MflI RGATCY 1 cut(s) 981
MluCI AATT 5 cut(s) 320, 421, 611, 780, 928
MlyI GAGTC 2 cut(s) 524, 725
MnlI CCTC 7 cut(s) 146, 265, 413, 416, 463, 589, 1095
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 425
MseI TTAA 3 cut(s) 633, 827, 897
MspI CCGG 3 cut(s) 96, 223, 426
MspR9I CCNGG 1 cut(s) 140
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 2 cut(s) 605, 699
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 764
NcoI CCATGG 1 cut(s) 315
NdeII GATC 6 cut(s) 175, 362, 384, 535, 776, 981
NlaIII CATG 7 cut(s) 157, 164, 319, 810, 823, 839, 1028
NlaIV GGNNCC 3 cut(s) 100, 394, 669
NmuCI GTSAC 1 cut(s) 728
NsbI TGCGCA 1 cut(s) 816
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 810, 823, 839
PaeI GCATGC 1 cut(s) 839
PciI ACATGT 1 cut(s) 819
PciSI GCTCTTC 1 cut(s) 125
PctI GAATGC 2 cut(s) 605, 699
PfeI GAWTC 2 cut(s) 586, 1011
PfoI TCCNGGA 1 cut(s) 138
PinAI ACCGGT 1 cut(s) 222
PkrI GCNGC 3 cut(s) 287, 310, 604
PleI GAGTC 2 cut(s) 523, 725
PpsI GAGTC 2 cut(s) 523, 725
Ppu21I YACGTR 1 cut(s) 859
PscI ACATGT 1 cut(s) 819
PshBI ATTAAT 1 cut(s) 633
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 100, 394, 669
PspPI GGNCC 1 cut(s) 668
PsuI RGATCY 1 cut(s) 981
RsaI GTAC 6 cut(s) 447, 466, 576, 658, 941, 972
RsaNI GTAC 6 cut(s) 446, 465, 575, 657, 940, 971
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 3 cut(s) 633, 827, 897
SatI GCNGC 3 cut(s) 286, 309, 603
Sau3AI GATC 6 cut(s) 175, 362, 384, 535, 776, 981
Sau96I GGNCC 1 cut(s) 668
ScaI AGTACT 2 cut(s) 466, 658
SchI GAGTC 2 cut(s) 524, 725
ScrFI CCNGG 1 cut(s) 140
SfaNI GCATC 1 cut(s) 320
SnaBI TACGTA 1 cut(s) 859
SphI GCATGC 1 cut(s) 839
Sse9I AATT 5 cut(s) 320, 421, 611, 780, 928
SsiI CCGC 4 cut(s) 54, 242, 474, 953
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 7 cut(s) 89, 169, 293, 334, 433, 661, 1054
TaiI ACGT 2 cut(s) 306, 861
TaqI TCGA 2 cut(s) 267, 361
TasI AATT 5 cut(s) 320, 421, 611, 780, 928
TatI WGTACW 4 cut(s) 445, 464, 574, 656
TfiI GAWTC 2 cut(s) 586, 1011
Tru1I TTAA 3 cut(s) 633, 827, 897
Tru9I TTAA 3 cut(s) 633, 827, 897
TscAI CASTG 2 cut(s) 109, 331
TseFI GTSAC 1 cut(s) 728
TseI GCWGC 3 cut(s) 285, 308, 602
Tsp45I GTSAC 1 cut(s) 728
TspDTI ATGAA 1 cut(s) 149
TspGWI ACGGA 4 cut(s) 161, 498, 504, 608
TspRI CASTG 2 cut(s) 109, 331
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 4 cut(s) 320, 421, 611, 928
XceI RCATGY 3 cut(s) 810, 823, 839
XspI CTAG 1 cut(s) 905
ZrmI AGTACT 2 cut(s) 466, 658
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.