Prupe.4G200500_v2.0.a1

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013441
Physical Location & Seq
Forward (+)
12464242 .. 12468340
4099 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G200500.1

Sequence Viewer

Length: 1050 bp
ATGGTGGGTTTGGAGGGCAAGAGAAAGGGAGCTATGGCAACTTACTGTGTGACAGGAGCCACTGGTTTTATAGGGTCTTGGCTGGTGAACACCCTCTTGGAAAGAGGCCACATGGTTCATGCCACAGTTCGTGATCCTGCCAAGTGTTTGCACCTATTATCATTGTGGACAAACACTGACCGGTTGAGATTGTTCCAAGCTGATTTGGGAGAGGAAGGAAGCTTCGACAAGGCTGTGAAGGGCTGTGATGGTGTATTTCATGTTGCAGCATCAATGGAATTCAGTGTTACTGTAGAAGAAAACATTGAGAGTTATGTGCAATCAAATATCATTGAACCTGCAATCAAAGGAACCTTGGATCTCCTCAAATCCTGTCTGAATTCTGGAACTGTGAAAAGGGTTGTGCTCACCTCTTCCATCAGCACTCTCACTGCCAAAGACAGCAAGGGAAATGCAAGAGCTGTTGTTGATGAATCTTGTCAAACACCAGTTGATCAAGTCTTGAGTGAAAAAGCAAATGGATGGGTTTATGTACTTTCAAAGATTCTCACAGAGGAAGCAGCATTTAGATTTGCAAATGAGAATGACATTAATCTGGTATCAGTGATAACAAGTACTGTTGGAGGTCCATTTCTCACTTCCAAGGTTCCATCAAGCATTCAAGTTCTTTTGTCACCAATAACAGGTGACTCTGAATTCCATGGGATATTATCTGCTGTAAATGCAAGAATGGGTTCAATTGCTTTAGTTCATGTTGAAGATATATGCAGTGCCCACATATTTTTAATGGAGCATGCCAAAGCTGAAGGTCCTTACATATGCGCTGCCCAAAGTTATCCAATATCTGAGTTGGTTAAGTATCTAGCTCAGGCATACCCTTGCTCAAATTCAAACAGATTTATGGAGGAGGAAATTAATGATGTAGTGCCTTCAGAAATCTCTTCAAAGAAGCTAACAGATTTGGGATTCAGTTTCAAGCATGGACTGGAAGATATTATCCATCAGACAATTACTACTTGTCTAGGTTATGGCTTTCTGCCTTCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

350

Amino Acids

37.79

Weight (kDa)

5.4

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 346
AclWI GGATC 2 cut(s) 128, 366
AcsI RAATTY 4 cut(s) 278, 379, 695, 886
AcuI CTGAAG 2 cut(s) 825, 915
AfaI GTAC 2 cut(s) 534, 616
AfiI CCNNNNNNNGG 1 cut(s) 683
AgeI ACCGGT 1 cut(s) 180
AgsI TTSAA 8 cut(s) 335, 540, 662, 738, 758, 891, 945, 976
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 7 cut(s) 32, 200, 222, 461, 803, 866, 952
AluI AGCT 7 cut(s) 32, 200, 222, 461, 803, 866, 952
Alw21I GWGCWC 1 cut(s) 408
AlwI GGATC 2 cut(s) 128, 366
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 3 cut(s) 266, 560, 824
ApoI RAATTY 4 cut(s) 278, 379, 695, 886
AseI ATTAAT 2 cut(s) 591, 915
AsiGI ACCGGT 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 733
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 2 cut(s) 626, 809
AsuHPI GGTGA 4 cut(s) 97, 400, 666, 698
AvaII GGWCC 2 cut(s) 626, 809
BaeGI GKGCMC 1 cut(s) 775
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 278, 572, 811
BccI CCATC 5 cut(s) 242, 425, 516, 658, 1008
BclI TGATCA 1 cut(s) 493
BfaI CTAG 2 cut(s) 863, 1022
BfmI CTRYAG 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 3 cut(s) 267, 561, 825
BlsI GCNGC 3 cut(s) 268, 562, 826
BmcAI AGTACT 1 cut(s) 616
Bme18I GGWCC 2 cut(s) 626, 809
BmgT120I GGNCC 2 cut(s) 626, 809
BmiI GGNNCC 3 cut(s) 58, 352, 648
BmsI GCATC 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 523
BsaJI CCNNGG 3 cut(s) 354, 642, 700
BsaWI WCCGGW 1 cut(s) 180
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse118I RCCGGY 1 cut(s) 180
Bse1I ACTGG 3 cut(s) 67, 488, 990
BseDI CCNNGG 3 cut(s) 354, 642, 700
BseGI GGATG 1 cut(s) 527
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMII CTCAG 2 cut(s) 837, 881
BseNI ACTGG 3 cut(s) 67, 488, 990
BseRI GAGGAG 2 cut(s) 353, 920
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 3 cut(s) 278, 572, 811
BshFI GGCC 1 cut(s) 108
BshTI ACCGGT 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 683
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 1 cut(s) 108
Bsp1286I GDGCHC 2 cut(s) 408, 775
Bsp143I GATC 3 cut(s) 133, 358, 493
Bsp19I CCATGG 1 cut(s) 700
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 838, 880
BspLI GGNNCC 3 cut(s) 58, 352, 648
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 2 cut(s) 128, 366
BsrFI RCCGGY 1 cut(s) 180
BsrI ACTGG 3 cut(s) 67, 488, 990
BssAI RCCGGY 1 cut(s) 180
BssECI CCNNGG 3 cut(s) 354, 642, 700
BssMI GATC 3 cut(s) 133, 358, 493
BssT1I CCWWGG 3 cut(s) 354, 642, 700
Bst4CI ACNGT 5 cut(s) 47, 127, 292, 391, 619
Bst6I CTCTTC 2 cut(s) 418, 946
BstC8I GCNNGC 1 cut(s) 795
BstDEI CTNAG 2 cut(s) 846, 867
BstDSI CCRYGG 1 cut(s) 700
BstF5I GGATG 1 cut(s) 527
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 3 cut(s) 136, 361, 496
BstMBI GATC 3 cut(s) 133, 358, 493
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstNSI RCATGY 1 cut(s) 797
BstSFI CTRYAG 1 cut(s) 291
BstSLI GKGCMC 1 cut(s) 775
BstV1I GCAGC 3 cut(s) 278, 572, 811
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 700
BtsCI GGATG 1 cut(s) 527
BtsI GCAGTG 2 cut(s) 429, 775
BtsIMutI CAGTG 6 cut(s) 60, 174, 289, 429, 609, 775
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 795
CfoI GCGC 1 cut(s) 824
Cfr10I RCCGGY 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 626, 809
Csp6I GTAC 2 cut(s) 533, 615
CspAI ACCGGT 1 cut(s) 180
CviAII CATG 7 cut(s) 112, 119, 260, 701, 752, 794, 980
CviQI GTAC 2 cut(s) 533, 615
DdeI CTNAG 2 cut(s) 846, 867
DpnI GATC 3 cut(s) 135, 360, 495
DpnII GATC 3 cut(s) 133, 358, 493
Eam1104I CTCTTC 2 cut(s) 418, 946
EarI CTCTTC 2 cut(s) 418, 946
Eco130I CCWWGG 3 cut(s) 354, 642, 700
Eco47I GGWCC 2 cut(s) 626, 809
Eco57I CTGAAG 2 cut(s) 825, 915
EcoO109I RGGNCCY 1 cut(s) 809
EcoRI GAATTC 3 cut(s) 278, 379, 695
EcoT14I CCWWGG 3 cut(s) 354, 642, 700
ErhI CCWWGG 3 cut(s) 354, 642, 700
FaeI CATG 7 cut(s) 115, 122, 263, 704, 755, 797, 983
FatI CATG 7 cut(s) 111, 118, 259, 700, 751, 793, 979
FauNDI CATATG 1 cut(s) 818
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 3 cut(s) 267, 561, 825
FokI GGATG 1 cut(s) 534
Fsp4HI GCNGC 3 cut(s) 267, 561, 825
FspBI CTAG 2 cut(s) 863, 1022
GlaI GCGC 1 cut(s) 823
GluI GCNGC 3 cut(s) 267, 561, 825
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 181
HhaI GCGC 1 cut(s) 824
Hin1II CATG 7 cut(s) 115, 122, 263, 704, 755, 797, 983
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HindIII AAGCTT 1 cut(s) 220
HinfI GANTC 4 cut(s) 473, 544, 689, 966
HpaII CCGG 1 cut(s) 181
HphI GGTGA 4 cut(s) 97, 400, 666, 698
Hpy166II GTNNAC 2 cut(s) 88, 168
Hpy188I TCNGA 5 cut(s) 378, 694, 847, 934, 1005
Hpy188III TCNNGA 3 cut(s) 131, 384, 502
Hpy8I GTNNAC 2 cut(s) 88, 168
HpyAV CCTTC 5 cut(s) 209, 232, 800, 939, 1050
HpyCH4III ACNGT 5 cut(s) 47, 127, 292, 391, 619
HpyCH4V TGCA 8 cut(s) 151, 266, 319, 341, 455, 575, 725, 768
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpyF3I CTNAG 2 cut(s) 846, 867
Hsp92II CATG 7 cut(s) 115, 122, 263, 704, 755, 797, 983
HspAI GCGC 1 cut(s) 822
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 3 cut(s) 133, 358, 493
LmnI GCTCC 3 cut(s) 29, 56, 790
Lsp1109I GCAGC 3 cut(s) 278, 572, 811
LweI GCATC 1 cut(s) 278
MaeI CTAG 2 cut(s) 863, 1022
MaeIII GTNAC 4 cut(s) 49, 286, 672, 686
MalI GATC 3 cut(s) 135, 360, 495
MboI GATC 3 cut(s) 133, 358, 493
MboII GAAGA 5 cut(s) 308, 405, 770, 933, 1001
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 2 cut(s) 408, 775
MluCI AATT 7 cut(s) 278, 379, 695, 738, 886, 912, 1008
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 1 cut(s) 601
MroXI GAANNNNTTC 1 cut(s) 733
MseI TTAA 4 cut(s) 591, 785, 855, 915
MspI CCGG 1 cut(s) 181
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 657
MwoI GCNNNNNNNGC 1 cut(s) 722
NcoI CCATGG 1 cut(s) 700
NdeI CATATG 1 cut(s) 818
NdeII GATC 3 cut(s) 133, 358, 493
NlaIII CATG 7 cut(s) 115, 122, 263, 704, 755, 797, 983
NlaIV GGNNCC 3 cut(s) 58, 352, 648
NmuCI GTSAC 3 cut(s) 49, 672, 686
NspI RCATGY 1 cut(s) 797
PaeI GCATGC 1 cut(s) 797
PctI GAATGC 1 cut(s) 657
PdmI GAANNNNTTC 1 cut(s) 733
PfeI GAWTC 3 cut(s) 473, 544, 966
PinAI ACCGGT 1 cut(s) 180
PkrI GCNGC 3 cut(s) 268, 562, 826
PleI GAGTC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 683
PpuMI RGGWCCY 1 cut(s) 809
PshBI ATTAAT 2 cut(s) 591, 915
Psp5II RGGWCCY 1 cut(s) 809
PspN4I GGNNCC 3 cut(s) 58, 352, 648
PspPI GGNCC 2 cut(s) 626, 809
PspPPI RGGWCCY 1 cut(s) 809
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 2 cut(s) 534, 616
RsaNI GTAC 2 cut(s) 533, 615
SaqAI TTAA 4 cut(s) 591, 785, 855, 915
SatI GCNGC 3 cut(s) 267, 561, 825
Sau3AI GATC 3 cut(s) 133, 358, 493
Sau96I GGNCC 2 cut(s) 626, 809
ScaI AGTACT 1 cut(s) 616
SchI GAGTC 1 cut(s) 683
SduI GDGCHC 2 cut(s) 408, 775
SfaNI GCATC 1 cut(s) 278
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 2 cut(s) 626, 809
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
SphI GCATGC 1 cut(s) 797
Sse9I AATT 7 cut(s) 278, 379, 695, 738, 886, 912, 1008
SspMI CTAG 2 cut(s) 863, 1022
StyI CCWWGG 3 cut(s) 354, 642, 700
TaaI ACNGT 5 cut(s) 47, 127, 292, 391, 619
TaqI TCGA 1 cut(s) 225
TasI AATT 7 cut(s) 278, 379, 695, 738, 886, 912, 1008
TatI WGTACW 2 cut(s) 532, 614
TfiI GAWTC 3 cut(s) 473, 544, 966
Tru1I TTAA 4 cut(s) 591, 785, 855, 915
Tru9I TTAA 4 cut(s) 591, 785, 855, 915
TscAI CASTG 6 cut(s) 67, 181, 289, 436, 609, 775
TseFI GTSAC 3 cut(s) 49, 672, 686
TseI GCWGC 3 cut(s) 266, 560, 824
Tsp45I GTSAC 3 cut(s) 49, 672, 686
TspDTI ATGAA 4 cut(s) 107, 248, 486, 740
TspRI CASTG 6 cut(s) 67, 181, 289, 436, 609, 775
VpaK11BI GGWCC 2 cut(s) 626, 809
VspI ATTAAT 2 cut(s) 591, 915
XapI RAATTY 4 cut(s) 278, 379, 695, 886
XceI RCATGY 1 cut(s) 797
XmnI GAANNNNTTC 1 cut(s) 733
XspI CTAG 2 cut(s) 863, 1022
ZrmI AGTACT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.