RLG00000033749

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013441
Physical Location & Seq
Forward (+)
31724654 .. 31729225
4572 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033749

Sequence Viewer

Length: 1452 bp
ATGCCAATATACCGATTACCTTCTATTCTACAGTTACAACCTAATATCACAGCCAAGTGTTTGCACCTTCTATCATTGTGGGTCGGCACTGACCGGTTGAGATTATTCCGAGCTGATTTGCAAGAAGAAGGAAGCTTCGACGAGGCTGTTAAGGGATGCGATGGCGTGTTTCACGTTGCAGCATCAATGCAATTCAGTGTTCCTGTGGAAGAGGACAATGAGGCTTATGTTCAATCAAATATCATCGACCCTGCAATCAAAGGAACCTTGGACCTCCTCAAATCCTGTGTGAAATCGGGTACCGTGAATAGGGTTGTGCTTACCTCTTCTATCAGTACTATGACTGCCAAAGATAGCAGTGGAAATCCAATCTCTGTTGTTGATGAATCTTGCAAAACTCCAGTTGATCGTGTCTGGGATGCGAAAGCAAGTGGATGGGTTTATGCTCTTTCAAAGATCCTCACAGAGGAAGCAGCATTTAGATTTGCAAGTGAGAATGGGATTAATCTTGTTTCAGTAATAACAAGTACTGTTGGAGGACCATTTCTCACTTCCAATGTTCCATCAAGCATTCGAGTTCTTTTGTCACCATTGACAGGTGACTCTGAGCTCTATGGGATATTAACTGCTGTAAATGCAAGAATGGGATCAATTGTATTAGTTCACATTGAGGACATATGCAATGCCCACATTTTTTTAATGGAGAATGATGAAGCAGAAGGAAGATACATATGTGCAGCCAAAAGTATTCCAATGTCTGAGTTGATTCTTTATCTAGATCAGGCATTTCCTTCCTCAAATTCACAGAGATGCAGGGTTGTGGTGGAGGAAGAGCAGAATAATCCAGCACCCTCTGAGATATCTTCAAAGAAGCTAAGTGATTTGGGTTTTAATTACAAGCATGGAATAGAAGATATTATTCACCACCTTGCCACCTGCGGGCACAGCAGCATCCCACCCCCTCCACCCACCGTTGCTGCAATAGCCCAGTCAGCGCTCCCTTGGCCTACCCACTGCCCCGTGCCCCCAATTGTCCATCTCGTGGTGCTGGAAAGCGAGCGTGGGACTAAGGTTCTGGAGGCCGAGTCGCTGCTGAAGGTGAGCCTGACCAAGGTTGATCTGGAGTCAGTGCATGTGTTCAACGAGGGATGGGCAAGCCAGTTAAGAGGGTTCATAAGGGAGGACGAGTACTTGCGGAGCTTGCACTTCAATTCGTTGAGAATCAAGGACGACGGATCTGTGGTCAACATGTCGCCAGCCGGAGCGAAGGTGACGATGATGTCGATGAAGATGACGGCGACGAGGATCATCACCGGGCATCGAGGCGACGAGGATCATCACCAGGCTGTACTGCTCCGCCAATCAGATCTGACAGAGACAGAGTTTCAGTCGGATTCCGAAGGCCTCGACATCGCCACCATCAGAGAAGTGAAGGAGGTTTGGTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

484

Amino Acids

52.95

Weight (kDa)

5.19

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 31 - 239 1.6e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 35 - 157 9.8e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
PUA_2 PF14306 344 - 413 4.8e-08 PUA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 944
AasI GACNNNNNNGTC 1 cut(s) 1279
Acc36I ACCTGC 1 cut(s) 944
Acc65I GGTACC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 299
AccB7I CCANNNNNTGG 1 cut(s) 1042
AciI CCGC 3 cut(s) 939, 1195, 1357
AclWI GGATC 5 cut(s) 451, 655, 1243, 1313, 1341
AcsI RAATTY 1 cut(s) 799
AcuI CTGAAG 1 cut(s) 1115
AfaI GTAC 5 cut(s) 301, 337, 529, 1190, 1350
AfeI AGCGCT 1 cut(s) 996
AfiI CCNNNNNNNGG 6 cut(s) 309, 466, 596, 939, 1042, 1111
AflIII ACRYGT 1 cut(s) 1248
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 5 cut(s) 233, 453, 867, 1141, 1210
AjnI CCWGG 1 cut(s) 1341
AluBI AGCT 5 cut(s) 113, 135, 610, 874, 1200
AluI AGCT 5 cut(s) 113, 135, 610, 874, 1200
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 1 cut(s) 1370
AlwI GGATC 5 cut(s) 451, 655, 1243, 1313, 1341
Aor51HI AGCGCT 1 cut(s) 996
AoxI GGCC 3 cut(s) 1004, 1080, 1402
ApeKI GCWGC 6 cut(s) 179, 473, 737, 948, 977, 1090
ApoI RAATTY 1 cut(s) 799
AseI ATTAAT 1 cut(s) 504
AsiGI ACCGGT 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 299
AspLEI GCGC 1 cut(s) 997
AspS9I GGNCC 2 cut(s) 271, 539
AsuC2I CCSGG 1 cut(s) 1315
AsuHPI GGTGA 7 cut(s) 579, 611, 914, 1111, 1282, 1303, 1331
AvaII GGWCC 2 cut(s) 271, 539
BaeGI GKGCMC 2 cut(s) 945, 1026
BaeI ACNNNNGTAYC 2 cut(s) 718, 751
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 612
BauI CACGAG 1 cut(s) 1040
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 6 cut(s) 191, 485, 749, 960, 964, 1077
BccI CCATC 6 cut(s) 155, 429, 571, 1044, 1143, 1427
BceAI ACGGC 1 cut(s) 1311
BciT130I CCWGG 1 cut(s) 1343
BcnI CCSGG 1 cut(s) 1315
BcoDI GTCTC 1 cut(s) 1370
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 998
BfuAI ACCTGC 1 cut(s) 944
BglII AGATCT 1 cut(s) 1366
BisI GCNGC 6 cut(s) 180, 474, 738, 949, 978, 1091
BlsI GCNGC 6 cut(s) 181, 475, 739, 950, 979, 1092
BmcAI AGTACT 3 cut(s) 337, 529, 1190
Bme1390I CCNGG 2 cut(s) 1315, 1343
Bme18I GGWCC 2 cut(s) 271, 539
BmgT120I GGNCC 2 cut(s) 271, 539
BmiI GGNNCC 2 cut(s) 265, 301
BmrFI CCNGG 2 cut(s) 1315, 1343
BmrI ACTGGG 1 cut(s) 982
BmsI GCATC 6 cut(s) 146, 191, 409, 800, 960, 1327
BmuI ACTGGG 1 cut(s) 982
BpmI CTGGAG 3 cut(s) 384, 1097, 1142
BpuMI CCSGG 1 cut(s) 1315
BsaJI CCNNGG 3 cut(s) 267, 1001, 1110
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 7 cut(s) 1070, 1100, 1172, 1202, 1254, 1284, 1427
Bsc4I CCNNNNNNNGG 6 cut(s) 309, 466, 596, 939, 1042, 1111
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 3 cut(s) 401, 988, 1159
Bse3DI GCAATG 1 cut(s) 688
BseBI CCWGG 1 cut(s) 1343
BseDI CCNNGG 3 cut(s) 267, 1001, 1110
BseGI GGATG 5 cut(s) 161, 424, 440, 951, 1154
BseLI CCNNNNNNNGG 6 cut(s) 309, 466, 596, 939, 1042, 1111
BseMI GCAATG 1 cut(s) 688
BseMII CTCAG 3 cut(s) 597, 750, 846
BseNI ACTGG 3 cut(s) 401, 988, 1159
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 2 cut(s) 945, 1026
BseXI GCAGC 6 cut(s) 191, 485, 749, 960, 964, 1077
BsgI GTGCAG 1 cut(s) 756
BshFI GGCC 3 cut(s) 1006, 1082, 1404
BshNI GGYRCC 1 cut(s) 299
BshTI ACCGGT 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 3 cut(s) 94, 1260, 1314
BslFI GGGAC 1 cut(s) 1078
BslI CCNNNNNNNGG 6 cut(s) 309, 466, 596, 939, 1042, 1111
BsmAI GTCTC 1 cut(s) 1370
BsmFI GGGAC 1 cut(s) 1078
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 3 cut(s) 1006, 1082, 1404
Bsp1286I GDGCHC 3 cut(s) 612, 945, 1026
Bsp143I GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
BspACI CCGC 3 cut(s) 939, 1195, 1357
BspANI GGCC 3 cut(s) 1006, 1082, 1404
BspCNI CTCAG 3 cut(s) 598, 751, 847
BspLI GGNNCC 2 cut(s) 265, 301
BspMI ACCTGC 1 cut(s) 944
BspPI GGATC 5 cut(s) 451, 655, 1243, 1313, 1341
BspQI GCTCTTC 1 cut(s) 825
BspT107I GGYRCC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 688
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 3 cut(s) 401, 988, 1159
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 3 cut(s) 267, 1001, 1110
BssMI GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
BssSI CACGAG 1 cut(s) 1040
BssT1I CCWWGG 3 cut(s) 267, 1001, 1110
Bst2BI CACGAG 1 cut(s) 1040
Bst2UI CCWGG 1 cut(s) 1343
Bst4CI ACNGT 4 cut(s) 33, 304, 532, 973
Bst6I CTCTTC 3 cut(s) 204, 331, 825
BstC8I GCNNGC 5 cut(s) 941, 1058, 1156, 1202, 1257
BstDEI CTNAG 5 cut(s) 606, 759, 855, 875, 1068
BstENI CCTNNNNNAGG 2 cut(s) 464, 1109
BstF5I GGATG 5 cut(s) 161, 424, 440, 951, 1154
BstH2I RGCGCY 1 cut(s) 998
BstHHI GCGC 1 cut(s) 997
BstKTI GATC 9 cut(s) 409, 459, 650, 781, 1120, 1238, 1308, 1336, 1369
BstMAI GTCTC 1 cut(s) 1370
BstMBI GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
BstMWI GCNNNNNNNGC 6 cut(s) 635, 945, 983, 992, 1003, 1201
BstNI CCWGG 1 cut(s) 1343
BstNSI RCATGY 2 cut(s) 1136, 1252
BstSCI CCNGG 2 cut(s) 1313, 1341
BstSFI CTRYAG 1 cut(s) 29
BstSLI GKGCMC 2 cut(s) 945, 1026
BstV1I GCAGC 6 cut(s) 191, 485, 749, 960, 964, 1077
BstX2I RGATCY 3 cut(s) 456, 1235, 1366
BstYI RGATCY 3 cut(s) 456, 1235, 1366
BsuRI GGCC 3 cut(s) 1006, 1082, 1404
BtgZI GCGATG 2 cut(s) 174, 1396
BtsCI GGATG 5 cut(s) 161, 424, 440, 951, 1154
BtsI GCAGTG 2 cut(s) 364, 1012
BtsIMutI CAGTG 5 cut(s) 87, 202, 364, 1012, 1134
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 5 cut(s) 941, 1058, 1156, 1202, 1257
CfoI GCGC 1 cut(s) 997
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 271, 539
Csp6I GTAC 5 cut(s) 300, 336, 528, 1189, 1349
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 3 cut(s) 902, 1133, 1249
CviQI GTAC 5 cut(s) 300, 336, 528, 1189, 1349
DdeI CTNAG 5 cut(s) 606, 759, 855, 875, 1068
DpnI GATC 9 cut(s) 408, 458, 649, 780, 1119, 1237, 1307, 1335, 1368
DpnII GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
DrdI GACNNNNNNGTC 1 cut(s) 1279
DseDI GACNNNNNNGTC 1 cut(s) 1279
Eam1104I CTCTTC 3 cut(s) 204, 331, 825
EarI CTCTTC 3 cut(s) 204, 331, 825
EciI GGCGGA 1 cut(s) 1346
Ecl136II GAGCTC 1 cut(s) 610
Eco130I CCWWGG 3 cut(s) 267, 1001, 1110
Eco147I AGGCCT 1 cut(s) 1404
Eco24I GRGCYC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 861
Eco47I GGWCC 2 cut(s) 271, 539
Eco47III AGCGCT 1 cut(s) 996
Eco53kI GAGCTC 1 cut(s) 610
Eco57I CTGAAG 1 cut(s) 1115
EcoICRI GAGCTC 1 cut(s) 610
EcoNI CCTNNNNNAGG 2 cut(s) 464, 1109
EcoRII CCWGG 1 cut(s) 1341
EcoRV GATATC 1 cut(s) 861
EcoT14I CCWWGG 3 cut(s) 267, 1001, 1110
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 3 cut(s) 267, 1001, 1110
FaeI CATG 3 cut(s) 905, 1136, 1252
FaqI GGGAC 1 cut(s) 1078
FatI CATG 3 cut(s) 901, 1132, 1248
FauI CCCGC 1 cut(s) 932
FauNDI CATATG 2 cut(s) 677, 731
Fnu4HI GCNGC 6 cut(s) 180, 474, 738, 949, 978, 1091
FokI GGATG 5 cut(s) 168, 431, 447, 938, 1161
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 6 cut(s) 180, 474, 738, 949, 978, 1091
FspBI CTAG 1 cut(s) 776
GlaI GCGC 1 cut(s) 996
GluI GCNGC 6 cut(s) 180, 474, 738, 949, 978, 1091
GsuI CTGGAG 3 cut(s) 384, 1097, 1142
HaeII RGCGCY 1 cut(s) 998
HaeIII GGCC 3 cut(s) 1006, 1082, 1404
HapII CCGG 3 cut(s) 94, 1260, 1314
HhaI GCGC 1 cut(s) 997
Hin1II CATG 3 cut(s) 905, 1136, 1252
Hin6I GCGC 1 cut(s) 995
HinP1I GCGC 1 cut(s) 995
HincII GTYRAC 1 cut(s) 1246
HindII GTYRAC 1 cut(s) 1246
HindIII AAGCTT 1 cut(s) 133
HinfI GANTC 7 cut(s) 386, 602, 766, 1085, 1124, 1221, 1394
HpaII CCGG 3 cut(s) 94, 1260, 1314
HphI GGTGA 7 cut(s) 579, 611, 914, 1111, 1282, 1303, 1331
Hpy166II GTNNAC 2 cut(s) 664, 1246
Hpy188I TCNGA 9 cut(s) 110, 607, 760, 856, 1366, 1371, 1393, 1399, 1424
Hpy188III TCNNGA 4 cut(s) 776, 1076, 1121, 1449
Hpy8I GTNNAC 2 cut(s) 664, 1246
Hpy99I CGWCG 4 cut(s) 143, 1235, 1303, 1331
HpyAV CCTTC 9 cut(s) 30, 77, 122, 713, 801, 1090, 1261, 1394, 1426
HpyCH4III ACNGT 4 cut(s) 33, 304, 532, 973
HpyCH4IV ACGT 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 6 cut(s) 635, 945, 983, 992, 1003, 1201
HpyF3I CTNAG 5 cut(s) 606, 759, 855, 875, 1068
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 3 cut(s) 905, 1136, 1252
HspAI GCGC 1 cut(s) 995
KpnI GGTACC 1 cut(s) 303
Kzo9I GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 4 cut(s) 1002, 1197, 1262, 1359
Lsp1109I GCAGC 6 cut(s) 191, 485, 749, 960, 964, 1077
LweI GCATC 6 cut(s) 146, 191, 409, 800, 960, 1327
MaeI CTAG 1 cut(s) 776
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 4 cut(s) 33, 585, 599, 1270
MalI GATC 9 cut(s) 408, 458, 649, 780, 1119, 1237, 1307, 1335, 1368
MboI GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
MboII GAAGA 8 cut(s) 137, 221, 318, 735, 842, 855, 923, 1300
MfeI CAATTG 2 cut(s) 651, 1029
MflI RGATCY 3 cut(s) 456, 1235, 1366
MhlI GDGCHC 3 cut(s) 612, 945, 1026
MluCI AATT 6 cut(s) 191, 651, 799, 892, 1029, 1210
MlyI GAGTC 3 cut(s) 596, 1094, 1133
MmeI TCCRAC 2 cut(s) 514, 1371
MseI TTAA 6 cut(s) 150, 504, 623, 698, 891, 1163
MslI CAYNNNNRTG 1 cut(s) 808
MspI CCGG 3 cut(s) 94, 1260, 1314
MspR9I CCNGG 2 cut(s) 1315, 1343
MunI CAATTG 2 cut(s) 651, 1029
Mva1269I GAATGC 1 cut(s) 570
MvaI CCWGG 1 cut(s) 1343
MwoI GCNNNNNNNGC 6 cut(s) 635, 945, 983, 992, 1003, 1201
NciI CCSGG 1 cut(s) 1315
NdeI CATATG 2 cut(s) 677, 731
NdeII GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
NlaIII CATG 3 cut(s) 905, 1136, 1252
NlaIV GGNNCC 2 cut(s) 265, 301
NmeAIII GCCGAG 1 cut(s) 1108
NmuCI GTSAC 3 cut(s) 585, 599, 1270
NspI RCATGY 2 cut(s) 1136, 1252
PaqCI CACCTGC 1 cut(s) 944
PceI AGGCCT 1 cut(s) 1404
PciI ACATGT 1 cut(s) 1248
PciSI GCTCTTC 1 cut(s) 825
PcsI WCGNNNNNNNCGW 1 cut(s) 1280
PctI GAATGC 1 cut(s) 570
PfeI GAWTC 4 cut(s) 386, 766, 1221, 1394
PflMI CCANNNNNTGG 1 cut(s) 1042
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 6 cut(s) 181, 475, 739, 950, 979, 1092
PleI GAGTC 3 cut(s) 596, 1093, 1132
PpsI GAGTC 3 cut(s) 596, 1093, 1132
PscI ACATGT 1 cut(s) 1248
PshBI ATTAAT 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 1341
PspGI CCWGG 1 cut(s) 1341
PspN4I GGNNCC 2 cut(s) 265, 301
PspPI GGNCC 2 cut(s) 271, 539
PsuI RGATCY 3 cut(s) 456, 1235, 1366
RsaI GTAC 5 cut(s) 301, 337, 529, 1190, 1350
RsaNI GTAC 5 cut(s) 300, 336, 528, 1189, 1349
RseI CAYNNNNRTG 1 cut(s) 808
SacI GAGCTC 1 cut(s) 612
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 6 cut(s) 150, 504, 623, 698, 891, 1163
SatI GCNGC 6 cut(s) 180, 474, 738, 949, 978, 1091
Sau3AI GATC 9 cut(s) 406, 456, 647, 778, 1117, 1235, 1305, 1333, 1366
Sau96I GGNCC 2 cut(s) 271, 539
ScaI AGTACT 3 cut(s) 337, 529, 1190
SchI GAGTC 3 cut(s) 596, 1094, 1133
ScrFI CCNGG 2 cut(s) 1315, 1343
SduI GDGCHC 3 cut(s) 612, 945, 1026
SfaNI GCATC 6 cut(s) 146, 191, 409, 800, 960, 1327
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 2 cut(s) 271, 539
SmiMI CAYNNNNRTG 1 cut(s) 808
Sse9I AATT 6 cut(s) 191, 651, 799, 892, 1029, 1210
SseBI AGGCCT 1 cut(s) 1404
SsiI CCGC 3 cut(s) 939, 1195, 1357
SspMI CTAG 1 cut(s) 776
SstI GAGCTC 1 cut(s) 612
StuI AGGCCT 1 cut(s) 1404
StyD4I CCNGG 2 cut(s) 1313, 1341
StyI CCWWGG 3 cut(s) 267, 1001, 1110
TaaI ACNGT 4 cut(s) 33, 304, 532, 973
TaiI ACGT 1 cut(s) 177
TaqI TCGA 6 cut(s) 138, 246, 574, 1283, 1321, 1407
TasI AATT 6 cut(s) 191, 651, 799, 892, 1029, 1210
TatI WGTACW 4 cut(s) 335, 527, 1188, 1348
TfiI GAWTC 4 cut(s) 386, 766, 1221, 1394
Tru1I TTAA 6 cut(s) 150, 504, 623, 698, 891, 1163
Tru9I TTAA 6 cut(s) 150, 504, 623, 698, 891, 1163
TscAI CASTG 5 cut(s) 94, 202, 364, 1019, 1134
TseFI GTSAC 3 cut(s) 585, 599, 1270
TseI GCWGC 6 cut(s) 179, 473, 737, 948, 977, 1090
Tsp45I GTSAC 3 cut(s) 585, 599, 1270
TspDTI ATGAA 4 cut(s) 399, 726, 1162, 1301
TspGWI ACGGA 1 cut(s) 1248
TspRI CASTG 5 cut(s) 94, 202, 364, 1019, 1134
Van91I CCANNNNNTGG 1 cut(s) 1042
VpaK11BI GGWCC 2 cut(s) 271, 539
VspI ATTAAT 1 cut(s) 504
XagI CCTNNNNNAGG 2 cut(s) 464, 1109
XapI RAATTY 1 cut(s) 799
XbaI TCTAGA 1 cut(s) 775
XceI RCATGY 2 cut(s) 1136, 1252
XcmI CCANNNNNNNNNTGG 1 cut(s) 1117
XspI CTAG 1 cut(s) 776
ZrmI AGTACT 3 cut(s) 337, 529, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.