RchiOBHm_Chr5g0037071

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013441
Physical Location & Seq
Forward (+)
31434357 .. 31436716
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31582

Sequence Viewer

Length: 966 bp
ATGCTTAATGATCAGTTACAACCTAAAATCACAGCCAAGTGTTTGCACCTTCTATCATTGTGGGCCGGCACTGACCGGTTGAGATTATTCCGAGCTGATTTGCAAGAAGAAGGAAGCTTCGACGAGGCTGTTAAGGGATGCGATGGCGTGTTTCACGTTGCAGCATCAATGCAATTCAGTATTCCTGTGGAAGAGAACATTGAGGCTTATGTTCAATCAAATATCATCGACCCTGCAATCAAAGGAACCTTGGACCTCCTCAAATCCTGTGTGAAATCGGGTACCGTGAATAGGGTTGTGCTTACCTCTTCTATCAGTACTATGACTGCCAAAGATAGCAGTGGAAATCCAATCTCTGTTGTTGACGAATCTTGCAAAACTCCAGTTGATCGTGTCTGGGATGAGAAAGCAAGTGGATGGGTTTATGCTCTTTCAAAGATCCTCACAGAGGAAGCAGCATTTAGATTTGCAAGTGAGAATGGGGTTAAGCTTGTTTCAGTAATAACATGTAATGTTGGAGGACCATTTCTTACTTCCAATGTTCCATCAAGCATTCGAGTTCTTTTGTCACCATTGACAGGTGACTCTGAGCTCTATGGGATATTAACTGCTGTAAATGCAAGAATGGGATCAATTGTATTAGTTCACATTGAGGACATATGCAATGCCCACATTTTTTTAATGGAGAATAATGCAGCAGAAGGAAGATATATATGTGCTGCCAAAAGTATTCCAATGTCTGAGTTGGTTCTTTATCTAGCTCAGGCATTTCCTTCCTCAAATTCACAGAGATGCAGGGTTGTGGTGGAGGAAGAACAGAATAATCCAGCACCCTCTGAGATATCTTCAAAGAAGCTAAGTGATTTGGGTTTTAATTACAAGCATGGTGTAGAAGATATTATTCACCAGTCAGTTTCTTCTTGTCTGGATTTTGGCCTTCTACCTCGTACTGGAAATTGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

321

Amino Acids

34.85

Weight (kDa)

5.14

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 25 - 233 4.9e-16 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 29 - 153 4.3e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 281
AclWI GGATC 2 cut(s) 433, 637
AcsI RAATTY 1 cut(s) 781
AfaI GTAC 3 cut(s) 283, 319, 949
AfiI CCNNNNNNNGG 4 cut(s) 291, 448, 578, 950
AflIII ACRYGT 1 cut(s) 506
AgeI ACCGGT 1 cut(s) 75
AgsI TTSAA 3 cut(s) 215, 435, 849
AluBI AGCT 6 cut(s) 95, 117, 490, 592, 761, 856
AluI AGCT 6 cut(s) 95, 117, 490, 592, 761, 856
Alw21I GWGCWC 1 cut(s) 594
AlwI GGATC 2 cut(s) 433, 637
AoxI GGCC 2 cut(s) 63, 934
ApeKI GCWGC 4 cut(s) 161, 455, 695, 719
ApoI RAATTY 1 cut(s) 781
AsiGI ACCGGT 1 cut(s) 75
Asp718I GGTACC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 63, 253, 521
AsuHPI GGTGA 3 cut(s) 561, 593, 896
AvaII GGWCC 2 cut(s) 253, 521
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 594
BbvI GCAGC 4 cut(s) 173, 467, 706, 707
BccI CCATC 3 cut(s) 137, 411, 553
BclI TGATCA 1 cut(s) 10
BfaI CTAG 2 cut(s) 758, 964
BisI GCNGC 4 cut(s) 162, 456, 696, 720
BlsI GCNGC 4 cut(s) 163, 457, 697, 721
BmcAI AGTACT 1 cut(s) 319
Bme18I GGWCC 2 cut(s) 253, 521
BmgT120I GGNCC 3 cut(s) 63, 253, 521
BmiI GGNNCC 2 cut(s) 247, 283
BmsI GCATC 3 cut(s) 128, 173, 782
BpmI CTGGAG 1 cut(s) 366
Bpu10I CCTNAGC 1 cut(s) 762
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 75
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 448, 578, 950
Bse118I RCCGGY 2 cut(s) 65, 75
Bse1I ACTGG 3 cut(s) 383, 907, 955
Bse3DI GCAATG 1 cut(s) 670
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 3 cut(s) 143, 406, 422
BseLI CCNNNNNNNGG 4 cut(s) 291, 448, 578, 950
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 4 cut(s) 579, 732, 776, 828
BseNI ACTGG 3 cut(s) 383, 907, 955
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 4 cut(s) 173, 467, 706, 707
BshFI GGCC 2 cut(s) 65, 936
BshNI GGYRCC 1 cut(s) 281
BshTI ACCGGT 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 594
BsiSI CCGG 2 cut(s) 66, 76
BslI CCNNNNNNNGG 4 cut(s) 291, 448, 578, 950
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 2 cut(s) 65, 936
Bsp1286I GDGCHC 1 cut(s) 594
Bsp143I GATC 4 cut(s) 10, 388, 438, 629
BspANI GGCC 2 cut(s) 65, 936
BspCNI CTCAG 4 cut(s) 580, 733, 775, 829
BspLI GGNNCC 2 cut(s) 247, 283
BspPI GGATC 2 cut(s) 433, 637
BspT107I GGYRCC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 670
BsrFI RCCGGY 2 cut(s) 65, 75
BsrI ACTGG 3 cut(s) 383, 907, 955
BssAI RCCGGY 2 cut(s) 65, 75
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 4 cut(s) 10, 388, 438, 629
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 2 cut(s) 186, 313
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 5 cut(s) 588, 741, 762, 837, 857
BstENI CCTNNNNNAGG 1 cut(s) 446
BstF5I GGATG 3 cut(s) 143, 406, 422
BstKTI GATC 4 cut(s) 13, 391, 441, 632
BstMBI GATC 4 cut(s) 10, 388, 438, 629
BstMWI GCNNNNNNNGC 1 cut(s) 617
BstNSI RCATGY 1 cut(s) 510
BstV1I GCAGC 4 cut(s) 173, 467, 706, 707
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 2 cut(s) 65, 936
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 3 cut(s) 143, 406, 422
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 2 cut(s) 69, 346
Cac8I GCNNGC 1 cut(s) 67
Cfr10I RCCGGY 2 cut(s) 65, 75
Cfr13I GGNCC 3 cut(s) 63, 253, 521
Csp6I GTAC 3 cut(s) 282, 318, 948
CspAI ACCGGT 1 cut(s) 75
CviAII CATG 2 cut(s) 507, 884
CviQI GTAC 3 cut(s) 282, 318, 948
DdeI CTNAG 5 cut(s) 588, 741, 762, 837, 857
DpnI GATC 4 cut(s) 12, 390, 440, 631
DpnII GATC 4 cut(s) 10, 388, 438, 629
Eam1104I CTCTTC 2 cut(s) 186, 313
EarI CTCTTC 2 cut(s) 186, 313
Ecl136II GAGCTC 1 cut(s) 592
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 594
Eco32I GATATC 1 cut(s) 843
Eco47I GGWCC 2 cut(s) 253, 521
Eco53kI GAGCTC 1 cut(s) 592
EcoICRI GAGCTC 1 cut(s) 592
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoRV GATATC 1 cut(s) 843
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 2 cut(s) 510, 887
FatI CATG 2 cut(s) 506, 883
FauNDI CATATG 1 cut(s) 659
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 4 cut(s) 162, 456, 696, 720
FokI GGATG 3 cut(s) 150, 413, 429
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 4 cut(s) 162, 456, 696, 720
FspBI CTAG 2 cut(s) 758, 964
GluI GCNGC 4 cut(s) 162, 456, 696, 720
GsuI CTGGAG 1 cut(s) 366
HaeIII GGCC 2 cut(s) 65, 936
HapII CCGG 2 cut(s) 66, 76
Hin1II CATG 2 cut(s) 510, 887
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 115, 488
HinfI GANTC 2 cut(s) 368, 584
HpaII CCGG 2 cut(s) 66, 76
HphI GGTGA 3 cut(s) 561, 593, 896
Hpy166II GTNNAC 2 cut(s) 364, 646
Hpy188I TCNGA 4 cut(s) 92, 589, 742, 838
Hpy188III TCNNGA 1 cut(s) 926
Hpy8I GTNNAC 2 cut(s) 364, 646
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 5 cut(s) 59, 104, 695, 783, 947
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 156
HpyF10VI GCNNNNNNNGC 1 cut(s) 617
HpyF3I CTNAG 5 cut(s) 588, 741, 762, 837, 857
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 2 cut(s) 510, 887
KpnI GGTACC 1 cut(s) 285
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 4 cut(s) 10, 388, 438, 629
Lsp1109I GCAGC 4 cut(s) 173, 467, 706, 707
LweI GCATC 3 cut(s) 128, 173, 782
MaeI CTAG 2 cut(s) 758, 964
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 4 cut(s) 15, 567, 581, 959
MalI GATC 4 cut(s) 12, 390, 440, 631
MboI GATC 4 cut(s) 10, 388, 438, 629
MboII GAAGA 8 cut(s) 119, 203, 300, 717, 824, 837, 905, 909
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 5 cut(s) 173, 633, 781, 874, 955
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 496
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 6 cut(s) 6, 132, 486, 605, 680, 873
MslI CAYNNNNRTG 1 cut(s) 790
MspI CCGG 2 cut(s) 66, 76
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 552
MwoI GCNNNNNNNGC 1 cut(s) 617
NaeI GCCGGC 1 cut(s) 67
NdeI CATATG 1 cut(s) 659
NdeII GATC 4 cut(s) 10, 388, 438, 629
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 2 cut(s) 510, 887
NlaIV GGNNCC 2 cut(s) 247, 283
NmuCI GTSAC 2 cut(s) 567, 581
NspI RCATGY 1 cut(s) 510
PciI ACATGT 1 cut(s) 506
PctI GAATGC 1 cut(s) 552
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 368
PinAI ACCGGT 1 cut(s) 75
PkrI GCNGC 4 cut(s) 163, 457, 697, 721
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PscI ACATGT 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 594
PspN4I GGNNCC 2 cut(s) 247, 283
PspPI GGNCC 3 cut(s) 63, 253, 521
PsuI RGATCY 1 cut(s) 438
RsaI GTAC 3 cut(s) 283, 319, 949
RsaNI GTAC 3 cut(s) 282, 318, 948
RseI CAYNNNNRTG 1 cut(s) 790
SacI GAGCTC 1 cut(s) 594
SaqAI TTAA 6 cut(s) 6, 132, 486, 605, 680, 873
SatI GCNGC 4 cut(s) 162, 456, 696, 720
Sau3AI GATC 4 cut(s) 10, 388, 438, 629
Sau96I GGNCC 3 cut(s) 63, 253, 521
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 1 cut(s) 578
SduI GDGCHC 1 cut(s) 594
SfaNI GCATC 3 cut(s) 128, 173, 782
SinI GGWCC 2 cut(s) 253, 521
SmiMI CAYNNNNRTG 1 cut(s) 790
Sse9I AATT 5 cut(s) 173, 633, 781, 874, 955
SspMI CTAG 2 cut(s) 758, 964
SstI GAGCTC 1 cut(s) 594
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 120, 228, 556
TasI AATT 5 cut(s) 173, 633, 781, 874, 955
TatI WGTACW 1 cut(s) 317
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 6 cut(s) 6, 132, 486, 605, 680, 873
Tru9I TTAA 6 cut(s) 6, 132, 486, 605, 680, 873
TscAI CASTG 2 cut(s) 76, 346
TseFI GTSAC 2 cut(s) 567, 581
TseI GCWGC 4 cut(s) 161, 455, 695, 719
Tsp45I GTSAC 2 cut(s) 567, 581
TspRI CASTG 2 cut(s) 76, 346
VpaK11BI GGWCC 2 cut(s) 253, 521
XagI CCTNNNNNAGG 1 cut(s) 446
XapI RAATTY 1 cut(s) 781
XceI RCATGY 1 cut(s) 510
XspI CTAG 2 cut(s) 758, 964
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.