Rh5CG286700

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013441
Physical Location & Seq
Forward (+)
31433146 .. 31442298
9153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG286700.1

Sequence Viewer

Length: 1110 bp
ATGGAGGGTTTAGAATCTGAGGGCAATAGAAATGGAGCTCCGGCAGTTTACTGTGTCACCGGAGCCACTGGTTATATCGGGTCCTGGTTGGTGAAGACTCTTCTTGAAAGAGGCCACACTGTTCATGCCACAGTCCGCGATCCTGGTTCATGCTTCCATCATGAAGTAATTTGTCGTTTTGGCGCCAAGGGTTTGCACCTTCTATCATTGTGGGTCGGCACTGACCGGTTGAGATTATTCCGAGCTGATTTGCAAGAAGAAGGAAGCTTCGACGAGGCTGTTAAGGGATGCGATGGTGTGTTTCACGTTGCAGCATCAATGCAATTCAGTGTTCCTGTGGAAGAGAACATTGAGGCTTATGTTCAATCAAATATCATCGACCCTGCAATCAAAGGAACCTTGGACCTCCTCAAATCCTGTGTGAAATCGGGTACCGTGAATAGGGTTGTGCTTACCTCTTCTATCAGTACTTTGACTGCCAAAGATAGCAGTGGAAATCCAATCTCTGTTGTTGATGAATCTTGCAAAACTCCAGTTGATCGTGTCTGGGATGCGAAAGCAAGTGGATGGGTTTATGCTCTTCCAAAGATCCTCACAGAGGAAGCAGCATTTAGATTTGCAAGTGAGAATGGGATTAATCTTGTTTCAGTAATAACAAGTACTGTTGGAGGACCATTTCTCACTTCCAATGTTCCATCAAGCATTCGAGTTCTTTTGTCACCATTGACAGGTGACTCTGAGCTCTATGGGATATTAACTGCTGTAAATGCAAGAATGGGATCAATTGTATTAGTTCACATTGAGGACTTATGCAATGCCCACATTTTTTTAATGGAGAATGATGAAGTAGAAGGAAGATACATATGTGCTGCCAAAAGTATTCCCATGTCTGAGTTGATTCTTTATCTAGCTCAGGCATTTCCTTCCTCAAATTCACAGAGATGCATGGTTGTGGTGGAGGAAGAGCAGAATAATCCAGCACCCTCTGAGATATCTTCAAATAAGCTAAGTGATCTGGGTTTTAATTACAAGCATGGAATAGAAGATATTATTCACCAGTCAGTTTCTTCTTGTCTGGATTTTGGCCTTCTACCTCGTACTGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

369

Amino Acids

39.88

Weight (kDa)

5.17

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 18 - 282 3e-24 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 19 - 154 7e-11 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 19 - 177 7.7e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 19 - 215 7.8e-10 Male sterility protein
NmrA PF05368 19 - 127 4.2e-09 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 2 cut(s) 182, 431
AccII CGCG 1 cut(s) 138
AciI CCGC 1 cut(s) 136
AclWI GGATC 3 cut(s) 134, 583, 787
AcsI RAATTY 1 cut(s) 931
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 4 cut(s) 433, 469, 661, 1099
AfiI CCNNNNNNNGG 4 cut(s) 441, 598, 728, 1100
AgeI ACCGGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 107, 365, 999
AjnI CCWGG 2 cut(s) 83, 142
AluBI AGCT 6 cut(s) 38, 245, 267, 742, 911, 1006
AluI AGCT 6 cut(s) 38, 245, 267, 742, 911, 1006
Alw21I GWGCWC 2 cut(s) 40, 744
AlwI GGATC 3 cut(s) 134, 583, 787
AoxI GGCC 2 cut(s) 112, 1084
ApeKI GCWGC 3 cut(s) 311, 605, 869
ApoI RAATTY 1 cut(s) 931
AseI ATTAAT 1 cut(s) 636
AsiGI ACCGGT 1 cut(s) 225
Asp718I GGTACC 1 cut(s) 431
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 3 cut(s) 81, 403, 671
AsuHPI GGTGA 5 cut(s) 49, 103, 711, 743, 1046
AvaII GGWCC 3 cut(s) 81, 403, 671
BaeI ACNNNNGTAYC 2 cut(s) 850, 883
BanI GGYRCC 2 cut(s) 182, 431
BanII GRGCYC 2 cut(s) 40, 744
BbsI GAAGAC 1 cut(s) 101
Bbv12I GWGCWC 2 cut(s) 40, 744
BbvI GCAGC 3 cut(s) 323, 617, 856
BccI CCATC 4 cut(s) 165, 287, 561, 703
BciT130I CCWGG 2 cut(s) 85, 144
BfaI CTAG 1 cut(s) 908
BfoI RGCGCY 1 cut(s) 186
BisI GCNGC 3 cut(s) 312, 606, 870
BlsI GCNGC 3 cut(s) 313, 607, 871
BmcAI AGTACT 2 cut(s) 469, 661
Bme1390I CCNGG 2 cut(s) 85, 144
Bme18I GGWCC 3 cut(s) 81, 403, 671
BmgT120I GGNCC 3 cut(s) 81, 403, 671
BmiI GGNNCC 5 cut(s) 64, 82, 184, 397, 433
BmrFI CCNGG 2 cut(s) 85, 144
BmsI GCATC 4 cut(s) 278, 323, 541, 932
BpiI GAAGAC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 912
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 186, 399
BsaWI WCCGGW 2 cut(s) 59, 225
Bsc4I CCNNNNNNNGG 4 cut(s) 441, 598, 728, 1100
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 4 cut(s) 73, 533, 1057, 1105
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 2 cut(s) 85, 144
BseDI CCNNGG 2 cut(s) 186, 399
BseGI GGATG 3 cut(s) 293, 556, 572
BseLI CCNNNNNNNGG 4 cut(s) 441, 598, 728, 1100
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 5 cut(s) 9, 729, 882, 926, 978
BseNI ACTGG 4 cut(s) 73, 533, 1057, 1105
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 3 cut(s) 323, 617, 856
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 114, 1086
BshNI GGYRCC 2 cut(s) 182, 431
BshTI ACCGGT 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 40, 744
BsiSI CCGG 3 cut(s) 41, 60, 226
BslI CCNNNNNNNGG 4 cut(s) 441, 598, 728, 1100
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 2 cut(s) 114, 1086
Bsp1286I GDGCHC 2 cut(s) 40, 744
Bsp143I GATC 5 cut(s) 139, 538, 588, 779, 1012
BspACI CCGC 1 cut(s) 136
BspANI GGCC 2 cut(s) 114, 1086
BspCNI CTCAG 5 cut(s) 10, 730, 883, 925, 979
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 5 cut(s) 64, 82, 184, 397, 433
BspPI GGATC 3 cut(s) 134, 583, 787
BspQI GCTCTTC 2 cut(s) 585, 957
BspT107I GGYRCC 2 cut(s) 182, 431
BsrDI GCAATG 1 cut(s) 820
BsrFI RCCGGY 1 cut(s) 225
BsrI ACTGG 4 cut(s) 73, 533, 1057, 1105
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 2 cut(s) 186, 399
BssMI GATC 5 cut(s) 139, 538, 588, 779, 1012
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 186, 399
Bst2UI CCWGG 2 cut(s) 85, 144
Bst4CI ACNGT 5 cut(s) 53, 121, 133, 436, 664
Bst6I CTCTTC 5 cut(s) 105, 336, 463, 585, 957
BstACI GRCGYC 1 cut(s) 183
BstDEI CTNAG 6 cut(s) 18, 738, 891, 912, 987, 1007
BstENI CCTNNNNNAGG 1 cut(s) 596
BstF5I GGATG 3 cut(s) 293, 556, 572
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 5 cut(s) 142, 541, 591, 782, 1015
BstMBI GATC 5 cut(s) 139, 538, 588, 779, 1012
BstMWI GCNNNNNNNGC 1 cut(s) 767
BstNI CCWGG 2 cut(s) 85, 144
BstSCI CCNGG 2 cut(s) 83, 142
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 323, 617, 856
BstV2I GAAGAC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 588
BstYI RGATCY 1 cut(s) 588
BsuRI GGCC 2 cut(s) 114, 1086
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 3 cut(s) 293, 556, 572
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 5 cut(s) 66, 117, 219, 334, 496
CciI TCATGA 1 cut(s) 160
CfoI GCGC 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 3 cut(s) 81, 403, 671
Csp6I GTAC 4 cut(s) 432, 468, 660, 1098
CspAI ACCGGT 1 cut(s) 225
CviAII CATG 6 cut(s) 125, 150, 161, 886, 946, 1034
CviQI GTAC 4 cut(s) 432, 468, 660, 1098
DdeI CTNAG 6 cut(s) 18, 738, 891, 912, 987, 1007
DinI GGCGCC 1 cut(s) 184
DpnI GATC 5 cut(s) 141, 540, 590, 781, 1014
DpnII GATC 5 cut(s) 139, 538, 588, 779, 1012
Eam1104I CTCTTC 5 cut(s) 105, 336, 463, 585, 957
EarI CTCTTC 5 cut(s) 105, 336, 463, 585, 957
Ecl136II GAGCTC 2 cut(s) 38, 742
Eco130I CCWWGG 2 cut(s) 186, 399
Eco24I GRGCYC 2 cut(s) 40, 744
Eco32I GATATC 1 cut(s) 993
Eco47I GGWCC 3 cut(s) 81, 403, 671
Eco53kI GAGCTC 2 cut(s) 38, 742
EcoICRI GAGCTC 2 cut(s) 38, 742
EcoNI CCTNNNNNAGG 1 cut(s) 596
EcoO109I RGGNCCY 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 83, 142
EcoRV GATATC 1 cut(s) 993
EcoT14I CCWWGG 2 cut(s) 186, 399
EcoT22I ATGCAT 1 cut(s) 947
EcoT38I GRGCYC 2 cut(s) 40, 744
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 186, 399
FaeI CATG 6 cut(s) 128, 153, 164, 889, 949, 1037
FatI CATG 6 cut(s) 124, 149, 160, 885, 945, 1033
FauNDI CATATG 1 cut(s) 863
Fnu4HI GCNGC 3 cut(s) 312, 606, 870
FokI GGATG 3 cut(s) 300, 563, 579
FriOI GRGCYC 2 cut(s) 40, 744
Fsp4HI GCNGC 3 cut(s) 312, 606, 870
FspBI CTAG 1 cut(s) 908
GlaI GCGC 1 cut(s) 184
GluI GCNGC 3 cut(s) 312, 606, 870
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 2 cut(s) 114, 1086
HapII CCGG 3 cut(s) 41, 60, 226
HhaI GCGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 6 cut(s) 128, 153, 164, 889, 949, 1037
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 5 cut(s) 14, 97, 518, 734, 898
HpaII CCGG 3 cut(s) 41, 60, 226
HphI GGTGA 5 cut(s) 49, 103, 711, 743, 1046
Hpy166II GTNNAC 2 cut(s) 49, 796
Hpy188I TCNGA 5 cut(s) 19, 242, 739, 892, 988
Hpy188III TCNNGA 3 cut(s) 104, 161, 1076
Hpy8I GTNNAC 2 cut(s) 49, 796
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 5 cut(s) 209, 254, 845, 933, 1097
HpyCH4III ACNGT 5 cut(s) 53, 121, 133, 436, 664
HpyCH4IV ACGT 1 cut(s) 306
HpyF10VI GCNNNNNNNGC 1 cut(s) 767
HpyF3I CTNAG 6 cut(s) 18, 738, 891, 912, 987, 1007
HpySE526I ACGT 1 cut(s) 306
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 6 cut(s) 128, 153, 164, 889, 949, 1037
HspAI GCGC 1 cut(s) 183
KasI GGCGCC 1 cut(s) 182
KpnI GGTACC 1 cut(s) 435
Kzo9I GATC 5 cut(s) 139, 538, 588, 779, 1012
LguI GCTCTTC 2 cut(s) 585, 957
LmnI GCTCC 3 cut(s) 35, 43, 62
Lsp1109I GCAGC 3 cut(s) 323, 617, 856
LweI GCATC 4 cut(s) 278, 323, 541, 932
MaeI CTAG 1 cut(s) 908
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 3 cut(s) 55, 717, 731
MalI GATC 5 cut(s) 141, 540, 590, 781, 1014
MboI GATC 5 cut(s) 139, 538, 588, 779, 1012
MfeI CAATTG 1 cut(s) 783
MflI RGATCY 1 cut(s) 588
MhlI GDGCHC 2 cut(s) 40, 744
MluCI AATT 5 cut(s) 168, 323, 783, 931, 1024
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 2 cut(s) 91, 728
MmeI TCCRAC 1 cut(s) 646
Mph1103I ATGCAT 1 cut(s) 947
MseI TTAA 5 cut(s) 282, 636, 755, 830, 1023
MslI CAYNNNNRTG 2 cut(s) 940, 950
MspI CCGG 3 cut(s) 41, 60, 226
MspR9I CCNGG 2 cut(s) 85, 144
MunI CAATTG 1 cut(s) 783
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 2 cut(s) 85, 144
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 767
NarI GGCGCC 1 cut(s) 183
NdeI CATATG 1 cut(s) 863
NdeII GATC 5 cut(s) 139, 538, 588, 779, 1012
NlaIII CATG 6 cut(s) 128, 153, 164, 889, 949, 1037
NlaIV GGNNCC 5 cut(s) 64, 82, 184, 397, 433
NmuCI GTSAC 3 cut(s) 55, 717, 731
NsiI ATGCAT 1 cut(s) 947
PagI TCATGA 1 cut(s) 160
PciSI GCTCTTC 2 cut(s) 585, 957
PctI GAATGC 1 cut(s) 702
PfeI GAWTC 3 cut(s) 14, 518, 898
PinAI ACCGGT 1 cut(s) 225
PkrI GCNGC 3 cut(s) 313, 607, 871
PleI GAGTC 2 cut(s) 91, 728
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 2 cut(s) 91, 728
PpuMI RGGWCCY 1 cut(s) 81
PshBI ATTAAT 1 cut(s) 636
Psp124BI GAGCTC 2 cut(s) 40, 744
Psp5II RGGWCCY 1 cut(s) 81
Psp6I CCWGG 2 cut(s) 83, 142
PspGI CCWGG 2 cut(s) 83, 142
PspN4I GGNNCC 5 cut(s) 64, 82, 184, 397, 433
PspPI GGNCC 3 cut(s) 81, 403, 671
PspPPI RGGWCCY 1 cut(s) 81
PsuI RGATCY 1 cut(s) 588
RsaI GTAC 4 cut(s) 433, 469, 661, 1099
RsaNI GTAC 4 cut(s) 432, 468, 660, 1098
RseI CAYNNNNRTG 2 cut(s) 940, 950
SacI GAGCTC 2 cut(s) 40, 744
SapI GCTCTTC 2 cut(s) 585, 957
SaqAI TTAA 5 cut(s) 282, 636, 755, 830, 1023
SatI GCNGC 3 cut(s) 312, 606, 870
Sau3AI GATC 5 cut(s) 139, 538, 588, 779, 1012
Sau96I GGNCC 3 cut(s) 81, 403, 671
ScaI AGTACT 2 cut(s) 469, 661
SchI GAGTC 2 cut(s) 91, 728
ScrFI CCNGG 2 cut(s) 85, 144
SduI GDGCHC 2 cut(s) 40, 744
SfaNI GCATC 4 cut(s) 278, 323, 541, 932
SfoI GGCGCC 1 cut(s) 184
SinI GGWCC 3 cut(s) 81, 403, 671
SmiMI CAYNNNNRTG 2 cut(s) 940, 950
Sse9I AATT 5 cut(s) 168, 323, 783, 931, 1024
SsiI CCGC 1 cut(s) 136
SspDI GGCGCC 1 cut(s) 182
SspMI CTAG 1 cut(s) 908
SstI GAGCTC 2 cut(s) 40, 744
StyD4I CCNGG 2 cut(s) 83, 142
StyI CCWWGG 2 cut(s) 186, 399
TaaI ACNGT 5 cut(s) 53, 121, 133, 436, 664
TaiI ACGT 1 cut(s) 309
TaqI TCGA 3 cut(s) 270, 378, 706
TasI AATT 5 cut(s) 168, 323, 783, 931, 1024
TatI WGTACW 2 cut(s) 467, 659
TfiI GAWTC 3 cut(s) 14, 518, 898
Tru1I TTAA 5 cut(s) 282, 636, 755, 830, 1023
Tru9I TTAA 5 cut(s) 282, 636, 755, 830, 1023
TscAI CASTG 5 cut(s) 73, 124, 226, 334, 496
TseFI GTSAC 3 cut(s) 55, 717, 731
TseI GCWGC 3 cut(s) 311, 605, 869
Tsp45I GTSAC 3 cut(s) 55, 717, 731
TspDTI ATGAA 5 cut(s) 113, 138, 177, 531, 858
TspRI CASTG 5 cut(s) 73, 124, 226, 334, 496
VpaK11BI GGWCC 3 cut(s) 81, 403, 671
VspI ATTAAT 1 cut(s) 636
XagI CCTNNNNNAGG 1 cut(s) 596
XapI RAATTY 1 cut(s) 931
XspI CTAG 1 cut(s) 908
ZrmI AGTACT 2 cut(s) 469, 661
Zsp2I ATGCAT 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.