Rh5DG262600

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013441
Physical Location & Seq
Forward (+)
31488904 .. 31507801
18898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG262600.1

Sequence Viewer

Length: 1077 bp
ATGGAGGGTTTAGAATCTGAGGGCAATAGAAATGGAGCTCCGGCAGTTTACTGTGTCACCGGAGCCACTGGTTATATCGGGTCTTGGTTGGTGAAGACTCTTCTTGAAAGAGGCCACACGGTTCATGCCACAGTCCGCGATCCTGCCAAGTGTTTGCACCTTCTATCATTGTGGGCCGGCACTGACCGGCTGAGATTATTCCGAGCTGATTTGCAAGAAGAAGGAAGCTTCGACGAGGCTGTTAAGGGATGCGATGGCGTGTTTCACGTTGCAGCATCAATGCAATTCAGTGTTCCTGTGGAAGAGAACATTGAGGCTTATGTTCAATCAAATATCATCGACCCTGCAATCAAAGGAACCTTGGACCTCCTCAAATCCTGTGTGAAATCGGGTACCGTGAATAGGGTTGTGCTTACCTCTTCTATCAGTACTATGACTGCCAAAGATAGCAATGGAAATCCAATCTCTGTTGTTGACGAATCTTGCAAAACTCCAGTTGATCGTGTCTGGGATGAGAAAGCAAGTGGATGGGTTTATGCTCTTTCAAAGATCCTCACAGAGGAAGCAGCATTTAGATTTGCAAGTGAGAATGGCGTTAAGCTTGTTTCAGTAATAACAAGTACTGTTGGAGGACCATTTGTCACTTCCAATGTTCCATCAAGCATTCGAGTTCTTTTGTCACCATTGACAGGTGACTCTGAGCTCTATGGGATATTAACTGCTGTAAATGCAAGAATGGGATCAATTGTATTAGTTCACATTGAGGACATATGCAATGCCCACATTTTTTTAATGGAGAATAATGAAGCAGAAGGAAGATACATATGTGCTGCCAAAAGTATTCCAATGTCTGAGTTGGTTCTTTATCTAGCTCAGGCATTTCCTTCCTCAAATTCACAGAGATGCAGGGTTGTGGCGGAGGAAGAACAGAATAATCCAGCACCCTCTGAGATATCTTCAAAGAAGCTAAGTGATTTGGGTTTTAATTACAAGCATGGAGTAGAAGATATTATTCACCAGTCAGTTTCTTCTTGTCTGGATTTTGGCCTTCTACCTCGTACTGGAAATTGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

358

Amino Acids

38.66

Weight (kDa)

5.19

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 15 - 148 8.7e-06 KR domain
Epimerase PF01370 18 - 270 5.1e-27 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 18 - 141 2.4e-06 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 19 - 287 5.1e-14 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 19 - 189 2.1e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
NmrA PF05368 19 - 114 6.4e-11 NmrA-like family
NAD_binding_4 PF07993 19 - 211 1.8e-09 Male sterility protein
NAD_binding_10 PF13460 21 - 145 5.8e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 136, 917
AclWI GGATC 3 cut(s) 134, 544, 748
AcsI RAATTY 1 cut(s) 892
AfaI GTAC 4 cut(s) 394, 430, 622, 1060
AfiI CCNNNNNNNGG 4 cut(s) 402, 559, 689, 1061
AgsI TTSAA 4 cut(s) 107, 326, 546, 960
AhdI GACNNNNNGTC 1 cut(s) 638
AluBI AGCT 7 cut(s) 38, 206, 228, 601, 703, 872, 967
AluI AGCT 7 cut(s) 38, 206, 228, 601, 703, 872, 967
Alw21I GWGCWC 2 cut(s) 40, 705
AlwI GGATC 3 cut(s) 134, 544, 748
AoxI GGCC 3 cut(s) 112, 174, 1045
ApeKI GCWGC 3 cut(s) 272, 566, 830
ApoI RAATTY 1 cut(s) 892
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 3 cut(s) 174, 364, 632
AsuHPI GGTGA 5 cut(s) 49, 103, 672, 704, 1007
AvaII GGWCC 2 cut(s) 364, 632
BaeI ACNNNNGTAYC 2 cut(s) 811, 844
BanI GGYRCC 1 cut(s) 392
BanII GRGCYC 2 cut(s) 40, 705
BbsI GAAGAC 1 cut(s) 101
Bbv12I GWGCWC 2 cut(s) 40, 705
BbvI GCAGC 3 cut(s) 284, 578, 817
BccI CCATC 3 cut(s) 248, 522, 664
BfaI CTAG 2 cut(s) 869, 1075
BisI GCNGC 3 cut(s) 273, 567, 831
BlsI GCNGC 3 cut(s) 274, 568, 832
BmcAI AGTACT 2 cut(s) 430, 622
Bme18I GGWCC 2 cut(s) 364, 632
BmeRI GACNNNNNGTC 1 cut(s) 638
BmgT120I GGNCC 3 cut(s) 174, 364, 632
BmiI GGNNCC 3 cut(s) 64, 358, 394
BmsI GCATC 3 cut(s) 239, 284, 893
BpiI GAAGAC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 873
BsaJI CCNNGG 1 cut(s) 360
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 4 cut(s) 402, 559, 689, 1061
Bse118I RCCGGY 2 cut(s) 176, 186
Bse1I ACTGG 4 cut(s) 73, 494, 1018, 1066
Bse3DI GCAATG 2 cut(s) 457, 781
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 3 cut(s) 254, 517, 533
BseLI CCNNNNNNNGG 4 cut(s) 402, 559, 689, 1061
BseMI GCAATG 2 cut(s) 457, 781
BseMII CTCAG 6 cut(s) 9, 182, 690, 843, 887, 939
BseNI ACTGG 4 cut(s) 73, 494, 1018, 1066
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 3 cut(s) 284, 578, 817
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 114, 176, 1047
BshNI GGYRCC 1 cut(s) 392
BsiHKAI GWGCWC 2 cut(s) 40, 705
BsiSI CCGG 4 cut(s) 41, 60, 177, 187
BslI CCNNNNNNNGG 4 cut(s) 402, 559, 689, 1061
BsmI GAATGC 1 cut(s) 663
BsnI GGCC 3 cut(s) 114, 176, 1047
Bsp1286I GDGCHC 2 cut(s) 40, 705
Bsp143I GATC 4 cut(s) 139, 499, 549, 740
BspACI CCGC 2 cut(s) 136, 917
BspANI GGCC 3 cut(s) 114, 176, 1047
BspCNI CTCAG 6 cut(s) 10, 183, 691, 844, 886, 940
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 64, 358, 394
BspPI GGATC 3 cut(s) 134, 544, 748
BspT107I GGYRCC 1 cut(s) 392
BsrDI GCAATG 2 cut(s) 457, 781
BsrFI RCCGGY 2 cut(s) 176, 186
BsrI ACTGG 4 cut(s) 73, 494, 1018, 1066
BssAI RCCGGY 2 cut(s) 176, 186
BssECI CCNNGG 1 cut(s) 360
BssMI GATC 4 cut(s) 139, 499, 549, 740
BssT1I CCWWGG 1 cut(s) 360
Bst4CI ACNGT 5 cut(s) 53, 121, 133, 397, 625
Bst6I CTCTTC 3 cut(s) 105, 297, 424
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 7 cut(s) 18, 191, 699, 852, 873, 948, 968
BstENI CCTNNNNNAGG 1 cut(s) 557
BstF5I GGATG 3 cut(s) 254, 517, 533
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 4 cut(s) 142, 502, 552, 743
BstMBI GATC 4 cut(s) 139, 499, 549, 740
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 284, 578, 817
BstV2I GAAGAC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 549
BstYI RGATCY 1 cut(s) 549
BsuRI GGCC 3 cut(s) 114, 176, 1047
BtgZI GCGATG 1 cut(s) 267
BtsCI GGATG 3 cut(s) 254, 517, 533
BtsIMutI CAGTG 3 cut(s) 66, 180, 295
Cac8I GCNNGC 1 cut(s) 178
Cfr10I RCCGGY 2 cut(s) 176, 186
Cfr13I GGNCC 3 cut(s) 174, 364, 632
Csp6I GTAC 4 cut(s) 393, 429, 621, 1059
CviAII CATG 2 cut(s) 125, 995
CviQI GTAC 4 cut(s) 393, 429, 621, 1059
DdeI CTNAG 7 cut(s) 18, 191, 699, 852, 873, 948, 968
DpnI GATC 4 cut(s) 141, 501, 551, 742
DpnII GATC 4 cut(s) 139, 499, 549, 740
DriI GACNNNNNGTC 1 cut(s) 638
Eam1104I CTCTTC 3 cut(s) 105, 297, 424
Eam1105I GACNNNNNGTC 1 cut(s) 638
EarI CTCTTC 3 cut(s) 105, 297, 424
EciI GGCGGA 1 cut(s) 932
Ecl136II GAGCTC 2 cut(s) 38, 703
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 2 cut(s) 40, 705
Eco32I GATATC 1 cut(s) 954
Eco47I GGWCC 2 cut(s) 364, 632
Eco53kI GAGCTC 2 cut(s) 38, 703
EcoICRI GAGCTC 2 cut(s) 38, 703
EcoNI CCTNNNNNAGG 1 cut(s) 557
EcoRV GATATC 1 cut(s) 954
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 2 cut(s) 40, 705
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 2 cut(s) 128, 998
FatI CATG 2 cut(s) 124, 994
FauNDI CATATG 2 cut(s) 770, 824
Fnu4HI GCNGC 3 cut(s) 273, 567, 831
FokI GGATG 3 cut(s) 261, 524, 540
FriOI GRGCYC 2 cut(s) 40, 705
Fsp4HI GCNGC 3 cut(s) 273, 567, 831
FspBI CTAG 2 cut(s) 869, 1075
GluI GCNGC 3 cut(s) 273, 567, 831
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 3 cut(s) 114, 176, 1047
HapII CCGG 4 cut(s) 41, 60, 177, 187
Hin1II CATG 2 cut(s) 128, 998
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HindIII AAGCTT 2 cut(s) 226, 599
HinfI GANTC 4 cut(s) 14, 97, 479, 695
HpaII CCGG 4 cut(s) 41, 60, 177, 187
HphI GGTGA 5 cut(s) 49, 103, 672, 704, 1007
Hpy166II GTNNAC 3 cut(s) 49, 475, 757
Hpy188I TCNGA 5 cut(s) 19, 203, 700, 853, 949
Hpy188III TCNNGA 2 cut(s) 104, 1037
Hpy8I GTNNAC 3 cut(s) 49, 475, 757
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 5 cut(s) 170, 215, 806, 894, 1058
HpyCH4III ACNGT 5 cut(s) 53, 121, 133, 397, 625
HpyCH4IV ACGT 1 cut(s) 267
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpyF3I CTNAG 7 cut(s) 18, 191, 699, 852, 873, 948, 968
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 2 cut(s) 128, 998
KpnI GGTACC 1 cut(s) 396
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Kzo9I GATC 4 cut(s) 139, 499, 549, 740
LmnI GCTCC 3 cut(s) 35, 43, 62
Lsp1109I GCAGC 3 cut(s) 284, 578, 817
LweI GCATC 3 cut(s) 239, 284, 893
MaeI CTAG 2 cut(s) 869, 1075
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 5 cut(s) 55, 640, 678, 692, 1070
MalI GATC 4 cut(s) 141, 501, 551, 742
MboI GATC 4 cut(s) 139, 499, 549, 740
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 1 cut(s) 549
MhlI GDGCHC 2 cut(s) 40, 705
MluCI AATT 5 cut(s) 284, 744, 892, 985, 1066
MlyI GAGTC 2 cut(s) 91, 689
MmeI TCCRAC 1 cut(s) 607
MroNI GCCGGC 1 cut(s) 176
MseI TTAA 5 cut(s) 243, 597, 716, 791, 984
MslI CAYNNNNRTG 1 cut(s) 901
MspI CCGG 4 cut(s) 41, 60, 177, 187
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 663
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 728
NaeI GCCGGC 1 cut(s) 178
NdeI CATATG 2 cut(s) 770, 824
NdeII GATC 4 cut(s) 139, 499, 549, 740
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 2 cut(s) 128, 998
NlaIV GGNNCC 3 cut(s) 64, 358, 394
NmuCI GTSAC 4 cut(s) 55, 640, 678, 692
PctI GAATGC 1 cut(s) 663
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 2 cut(s) 14, 479
PkrI GCNGC 3 cut(s) 274, 568, 832
PleI GAGTC 2 cut(s) 91, 689
PpsI GAGTC 2 cut(s) 91, 689
Psp124BI GAGCTC 2 cut(s) 40, 705
PspN4I GGNNCC 3 cut(s) 64, 358, 394
PspPI GGNCC 3 cut(s) 174, 364, 632
PsuI RGATCY 1 cut(s) 549
RsaI GTAC 4 cut(s) 394, 430, 622, 1060
RsaNI GTAC 4 cut(s) 393, 429, 621, 1059
RseI CAYNNNNRTG 1 cut(s) 901
SacI GAGCTC 2 cut(s) 40, 705
SaqAI TTAA 5 cut(s) 243, 597, 716, 791, 984
SatI GCNGC 3 cut(s) 273, 567, 831
Sau3AI GATC 4 cut(s) 139, 499, 549, 740
Sau96I GGNCC 3 cut(s) 174, 364, 632
ScaI AGTACT 2 cut(s) 430, 622
SchI GAGTC 2 cut(s) 91, 689
SduI GDGCHC 2 cut(s) 40, 705
SfaNI GCATC 3 cut(s) 239, 284, 893
SinI GGWCC 2 cut(s) 364, 632
SmiMI CAYNNNNRTG 1 cut(s) 901
Sse9I AATT 5 cut(s) 284, 744, 892, 985, 1066
SsiI CCGC 2 cut(s) 136, 917
SspMI CTAG 2 cut(s) 869, 1075
SstI GAGCTC 2 cut(s) 40, 705
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 5 cut(s) 53, 121, 133, 397, 625
TaiI ACGT 1 cut(s) 270
TaqI TCGA 3 cut(s) 231, 339, 667
TasI AATT 5 cut(s) 284, 744, 892, 985, 1066
TatI WGTACW 2 cut(s) 428, 620
TfiI GAWTC 2 cut(s) 14, 479
Tru1I TTAA 5 cut(s) 243, 597, 716, 791, 984
Tru9I TTAA 5 cut(s) 243, 597, 716, 791, 984
TscAI CASTG 3 cut(s) 73, 187, 295
TseFI GTSAC 4 cut(s) 55, 640, 678, 692
TseI GCWGC 3 cut(s) 272, 566, 830
Tsp45I GTSAC 4 cut(s) 55, 640, 678, 692
TspDTI ATGAA 2 cut(s) 113, 819
TspRI CASTG 3 cut(s) 73, 187, 295
VpaK11BI GGWCC 2 cut(s) 364, 632
XagI CCTNNNNNAGG 1 cut(s) 557
XapI RAATTY 1 cut(s) 892
XspI CTAG 2 cut(s) 869, 1075
ZrmI AGTACT 2 cut(s) 430, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.