FvH4_3g21610

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013441
Physical Location & Seq
Forward (+)
14699399 .. 14704230
4832 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21610.t1

Sequence Viewer

Length: 1065 bp
ATGGAGGGTTCAGAATCAGAGGGCAATAGAAATGGAGCTCCGGCAGTTTACTGTGTCACCGGAGCCACCGGTTATATCGGATCTTGGTTGGTGAAGACTCTTCTTGAAAGAGGCCACACGGTTCATGCCACAGTACGCGATCCTGCCAAGTGTTTGCACCTATTACCATTATGGGCCGGCAGTGACCGGTTGAGATTACTCCGAGCTGATTTGGAAGAAGAAGGAAGCTTCGATGAGGCTGTTAAGGGCTGTGATGGCGTTTTTCATGTTGCAGCATCCATGCAATTCAGTGTTCCTGCGGCAGAGAACAGTGAGGGTTATGTTCAATTGAATATCATCGACCCTGCAATCAAAGGAACCTTGGACCTCCTCAAATCCTGTCTGAAATCGGGTACCGTGAATAGAGTTGTGCTAACCTCTTCCATTAGTACTATGACTGCCAAAGATAGCAGTGGCAATCCAATCTCTGTAGTTGATGAATCTTGCAAAACTCCAGTTGATCGCGTCTCGGATGCAAAAGCAAGTGGATGGGTTTATGCTCTTTCAAAGATCCTTACGGAGGAAGCAGCATTTAGATTTGCAAGTGAAAATGGGATCAATCTTGTTGCAGTAATTACAAGTACTGTTGGAGGACCATTTCTCACTTCCAATGTTCCATTAAGCATTCGAGTTCTTTTGTCACCATTAACAGGTGACTCTGAGCTCTATGGGATCTTATCTGCTGTAAATGCAAGAATGGGATCAATTGCTTTAGTTCACATTGAGGACATATGTGATGCCCACATTTTTTTGATGGAGCATGATGAAGCAGAAGGTAGATATATATGTGCTGCCCAAAGTTGTCCAATGTCTAAGTTGGTTCTTTATCTAGCTCAGGCATTTCCTTCCTCAGATTCAAAGAGCATTGTGGTGGAGGAAGAACAGAATAATCCAGTACCCTCTGAGTTATCTTCAAAGAAGCTAGGAGATTTAGGTTTCAATTACAAGCATGGAATAGAAGATATTATTAACCAGTCAGTTTCTTCTTGTCTGGGTTTTGGCCTTCTACCTCGTACGGCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

355

Amino Acids

37.78

Weight (kDa)

5.1

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 18 - 270 2.9e-27 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 19 - 279 2e-13 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 19 - 188 2.6e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 19 - 211 9.1e-10 Male sterility protein
NmrA PF05368 19 - 95 5.4e-10 NmrA-like family
NAD_binding_10 PF13460 21 - 145 4e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccII CGCG 2 cut(s) 138, 504
AciI CCGC 1 cut(s) 299
AclWI GGATC 6 cut(s) 88, 134, 544, 602, 719, 748
AfaI GTAC 6 cut(s) 135, 394, 430, 622, 936, 1054
AfiI CCNNNNNNNGG 2 cut(s) 559, 689
AgeI ACCGGT 2 cut(s) 68, 186
AgsI TTSAA 7 cut(s) 107, 326, 331, 546, 897, 954, 979
AluBI AGCT 6 cut(s) 38, 206, 228, 703, 872, 961
AluI AGCT 6 cut(s) 38, 206, 228, 703, 872, 961
Alw21I GWGCWC 2 cut(s) 40, 705
Alw26I GTCTC 1 cut(s) 511
AlwI GGATC 6 cut(s) 88, 134, 544, 602, 719, 748
AoxI GGCC 3 cut(s) 112, 174, 1039
ApeKI GCWGC 3 cut(s) 272, 566, 830
AsiGI ACCGGT 2 cut(s) 68, 186
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 3 cut(s) 174, 364, 632
AsuHPI GGTGA 4 cut(s) 49, 103, 672, 704
AvaII GGWCC 2 cut(s) 364, 632
BanI GGYRCC 1 cut(s) 392
BanII GRGCYC 2 cut(s) 40, 705
BbsI GAAGAC 1 cut(s) 101
Bbv12I GWGCWC 2 cut(s) 40, 705
BbvI GCAGC 3 cut(s) 284, 578, 817
BccI CCATC 3 cut(s) 248, 522, 787
BcoDI GTCTC 1 cut(s) 511
BfaI CTAG 2 cut(s) 869, 962
BfmI CTRYAG 1 cut(s) 468
BisI GCNGC 4 cut(s) 273, 300, 567, 831
BlsI GCNGC 4 cut(s) 274, 301, 568, 832
BmcAI AGTACT 2 cut(s) 430, 622
Bme18I GGWCC 2 cut(s) 364, 632
BmgT120I GGNCC 3 cut(s) 174, 364, 632
BmiI GGNNCC 3 cut(s) 64, 358, 394
BmsI GCATC 3 cut(s) 284, 502, 766
BpiI GAAGAC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 873
BsaJI CCNNGG 1 cut(s) 360
BsaWI WCCGGW 3 cut(s) 59, 68, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 559, 689
Bse118I RCCGGY 3 cut(s) 68, 176, 186
Bse1I ACTGG 3 cut(s) 494, 932, 1012
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 3 cut(s) 275, 517, 533
BseLI CCNNNNNNNGG 2 cut(s) 559, 689
BseMII CTCAG 4 cut(s) 690, 887, 903, 933
BseNI ACTGG 3 cut(s) 494, 932, 1012
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 3 cut(s) 284, 578, 817
Bsh1236I CGCG 2 cut(s) 138, 504
BshFI GGCC 3 cut(s) 114, 176, 1041
BshNI GGYRCC 1 cut(s) 392
BshTI ACCGGT 2 cut(s) 68, 186
BsiHKAI GWGCWC 2 cut(s) 40, 705
BsiSI CCGG 5 cut(s) 41, 60, 69, 177, 187
BsiWI CGTACG 1 cut(s) 1052
BslI CCNNNNNNNGG 2 cut(s) 559, 689
BsmAI GTCTC 1 cut(s) 511
BsmBI CGTCTC 1 cut(s) 511
BsmI GAATGC 1 cut(s) 663
BsnI GGCC 3 cut(s) 114, 176, 1041
Bsp1286I GDGCHC 2 cut(s) 40, 705
Bsp143I GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
BspACI CCGC 1 cut(s) 299
BspANI GGCC 3 cut(s) 114, 176, 1041
BspCNI CTCAG 4 cut(s) 691, 886, 902, 934
BspFNI CGCG 2 cut(s) 138, 504
BspLI GGNNCC 3 cut(s) 64, 358, 394
BspPI GGATC 6 cut(s) 88, 134, 544, 602, 719, 748
BspT107I GGYRCC 1 cut(s) 392
BsrFI RCCGGY 3 cut(s) 68, 176, 186
BsrI ACTGG 3 cut(s) 494, 932, 1012
BssAI RCCGGY 3 cut(s) 68, 176, 186
BssECI CCNNGG 1 cut(s) 360
BssMI GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
BssT1I CCWWGG 1 cut(s) 360
Bst4CI ACNGT 6 cut(s) 53, 121, 133, 311, 397, 625
Bst6I CTCTTC 2 cut(s) 105, 424
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 5 cut(s) 699, 852, 873, 889, 942
BstENI CCTNNNNNAGG 1 cut(s) 557
BstF5I GGATG 3 cut(s) 275, 517, 533
BstFNI CGCG 2 cut(s) 138, 504
BstKTI GATC 7 cut(s) 83, 142, 502, 552, 597, 714, 743
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
BstMWI GCNNNNNNNGC 2 cut(s) 255, 728
BstSFI CTRYAG 1 cut(s) 468
BstUI CGCG 2 cut(s) 138, 504
BstV1I GCAGC 3 cut(s) 284, 578, 817
BstV2I GAAGAC 1 cut(s) 101
BstX2I RGATCY 3 cut(s) 80, 549, 711
BstYI RGATCY 3 cut(s) 80, 549, 711
BsuRI GGCC 3 cut(s) 114, 176, 1041
BtsCI GGATG 3 cut(s) 275, 517, 533
BtsI GCAGTG 2 cut(s) 187, 457
BtsIMutI CAGTG 4 cut(s) 187, 295, 316, 457
Cac8I GCNNGC 1 cut(s) 178
Cfr10I RCCGGY 3 cut(s) 68, 176, 186
Cfr13I GGNCC 3 cut(s) 174, 364, 632
CseI GACGC 1 cut(s) 493
Csp6I GTAC 6 cut(s) 134, 393, 429, 621, 935, 1053
CspAI ACCGGT 2 cut(s) 68, 186
CviAII CATG 5 cut(s) 125, 266, 280, 800, 989
CviQI GTAC 6 cut(s) 134, 393, 429, 621, 935, 1053
DdeI CTNAG 5 cut(s) 699, 852, 873, 889, 942
DpnI GATC 7 cut(s) 82, 141, 501, 551, 596, 713, 742
DpnII GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
Eam1104I CTCTTC 2 cut(s) 105, 424
EarI CTCTTC 2 cut(s) 105, 424
Ecl136II GAGCTC 2 cut(s) 38, 703
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 2 cut(s) 40, 705
Eco47I GGWCC 2 cut(s) 364, 632
Eco53kI GAGCTC 2 cut(s) 38, 703
EcoICRI GAGCTC 2 cut(s) 38, 703
EcoNI CCTNNNNNAGG 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 2 cut(s) 40, 705
ErhI CCWWGG 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 5 cut(s) 128, 269, 283, 803, 992
FatI CATG 5 cut(s) 124, 265, 279, 799, 988
FauNDI CATATG 1 cut(s) 770
Fnu4HI GCNGC 4 cut(s) 273, 300, 567, 831
FokI GGATG 3 cut(s) 262, 524, 540
FriOI GRGCYC 2 cut(s) 40, 705
Fsp4HI GCNGC 4 cut(s) 273, 300, 567, 831
FspBI CTAG 2 cut(s) 869, 962
GluI GCNGC 4 cut(s) 273, 300, 567, 831
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 3 cut(s) 114, 176, 1041
HapII CCGG 5 cut(s) 41, 60, 69, 177, 187
HgaI GACGC 1 cut(s) 493
Hin1II CATG 5 cut(s) 128, 269, 283, 803, 992
HindIII AAGCTT 1 cut(s) 226
HinfI GANTC 5 cut(s) 14, 97, 479, 695, 893
HpaII CCGG 5 cut(s) 41, 60, 69, 177, 187
HphI GGTGA 4 cut(s) 49, 103, 672, 704
Hpy166II GTNNAC 2 cut(s) 49, 757
Hpy188I TCNGA 9 cut(s) 13, 19, 80, 203, 384, 511, 700, 892, 943
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 2 cut(s) 49, 757
HpyAV CCTTC 4 cut(s) 215, 806, 894, 1052
HpyCH4III ACNGT 6 cut(s) 53, 121, 133, 311, 397, 625
HpyCH4V TGCA 9 cut(s) 157, 272, 283, 347, 486, 515, 581, 608, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 255, 728
HpyF3I CTNAG 5 cut(s) 699, 852, 873, 889, 942
Hsp92II CATG 5 cut(s) 128, 269, 283, 803, 992
KpnI GGTACC 1 cut(s) 396
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Kzo9I GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
LmnI GCTCC 4 cut(s) 35, 43, 62, 796
Lsp1109I GCAGC 3 cut(s) 284, 578, 817
LweI GCATC 3 cut(s) 284, 502, 766
MaeI CTAG 2 cut(s) 869, 962
MaeIII GTNAC 4 cut(s) 55, 182, 678, 692
MalI GATC 7 cut(s) 82, 141, 501, 551, 596, 713, 742
MboI GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
MboII GAAGA 9 cut(s) 92, 106, 227, 230, 411, 929, 942, 1010, 1014
MfeI CAATTG 2 cut(s) 326, 744
MflI RGATCY 3 cut(s) 80, 549, 711
MhlI GDGCHC 2 cut(s) 40, 705
MluCI AATT 5 cut(s) 284, 326, 612, 744, 979
MlyI GAGTC 2 cut(s) 91, 689
MmeI TCCRAC 1 cut(s) 607
MroNI GCCGGC 1 cut(s) 176
MseI TTAA 4 cut(s) 243, 659, 686, 1008
MslI CAYNNNNRTG 1 cut(s) 908
MspI CCGG 5 cut(s) 41, 60, 69, 177, 187
MunI CAATTG 2 cut(s) 326, 744
Mva1269I GAATGC 1 cut(s) 663
MvnI CGCG 2 cut(s) 138, 504
MwoI GCNNNNNNNGC 2 cut(s) 255, 728
NaeI GCCGGC 1 cut(s) 178
NdeI CATATG 1 cut(s) 770
NdeII GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 5 cut(s) 128, 269, 283, 803, 992
NlaIV GGNNCC 3 cut(s) 64, 358, 394
NmuCI GTSAC 4 cut(s) 55, 182, 678, 692
PctI GAATGC 1 cut(s) 663
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 3 cut(s) 14, 479, 893
Pfl23II CGTACG 1 cut(s) 1052
PinAI ACCGGT 2 cut(s) 68, 186
PkrI GCNGC 4 cut(s) 274, 301, 568, 832
PleI GAGTC 2 cut(s) 91, 689
PpsI GAGTC 2 cut(s) 91, 689
Psp124BI GAGCTC 2 cut(s) 40, 705
PspLI CGTACG 1 cut(s) 1052
PspN4I GGNNCC 3 cut(s) 64, 358, 394
PspPI GGNCC 3 cut(s) 174, 364, 632
PsuI RGATCY 3 cut(s) 80, 549, 711
RsaI GTAC 6 cut(s) 135, 394, 430, 622, 936, 1054
RsaNI GTAC 6 cut(s) 134, 393, 429, 621, 935, 1053
RseI CAYNNNNRTG 1 cut(s) 908
SacI GAGCTC 2 cut(s) 40, 705
SaqAI TTAA 4 cut(s) 243, 659, 686, 1008
SatI GCNGC 4 cut(s) 273, 300, 567, 831
Sau3AI GATC 7 cut(s) 80, 139, 499, 549, 594, 711, 740
Sau96I GGNCC 3 cut(s) 174, 364, 632
ScaI AGTACT 2 cut(s) 430, 622
SchI GAGTC 2 cut(s) 91, 689
SduI GDGCHC 2 cut(s) 40, 705
SfaNI GCATC 3 cut(s) 284, 502, 766
SfcI CTRYAG 1 cut(s) 468
SinI GGWCC 2 cut(s) 364, 632
SmiMI CAYNNNNRTG 1 cut(s) 908
Sse9I AATT 5 cut(s) 284, 326, 612, 744, 979
SsiI CCGC 1 cut(s) 299
SspMI CTAG 2 cut(s) 869, 962
SstI GAGCTC 2 cut(s) 40, 705
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 6 cut(s) 53, 121, 133, 311, 397, 625
TaqI TCGA 3 cut(s) 231, 339, 667
TasI AATT 5 cut(s) 284, 326, 612, 744, 979
TatI WGTACW 2 cut(s) 428, 620
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 3 cut(s) 14, 479, 893
Tru1I TTAA 4 cut(s) 243, 659, 686, 1008
Tru9I TTAA 4 cut(s) 243, 659, 686, 1008
TscAI CASTG 4 cut(s) 187, 295, 316, 457
TseFI GTSAC 4 cut(s) 55, 182, 678, 692
TseI GCWGC 3 cut(s) 272, 566, 830
Tsp45I GTSAC 4 cut(s) 55, 182, 678, 692
TspDTI ATGAA 4 cut(s) 113, 254, 492, 819
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 4 cut(s) 187, 295, 316, 457
VpaK11BI GGWCC 2 cut(s) 364, 632
XagI CCTNNNNNAGG 1 cut(s) 557
XspI CTAG 2 cut(s) 869, 962
ZrmI AGTACT 2 cut(s) 430, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.