AT4G27740

Yippee zinc-binding/DNA-binding /Mis18, centromere assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006258
Physical Location & Seq
Forward (+)
13839038 .. 13839842
805 bp
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UTR
Exon/CDS
Intron
AT4G27740.1

Sequence Viewer

Length: 318 bp
ATGGCAGCAAACAAAACTCTTCCTACGTATTTTTGCAGAAACTGCGAGAATCCACTAGCTCTCGGTGAAGATCTCATCTCCAAAAAATTTGTGGGAGCTTCAGGGCCAGCGTTTATGTTTTCACACGCGATGAACGTGGTGGTTGGACCGAAGATTGGGAGGAAACTGATAACCGGATCGTATGTAGTGGCAGATGTGATGTGCAGTAAGTGTGGTGAGACGTTGGGTTGGAAATATGTTGAGACATTCGATCTTAAACAGAGGTATAAAGAAGGCATGTTTGTCATCGAGAAGCTCAAGTTGACCAAAAGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

105

Amino Acids

11.78

Weight (kDa)

9.37

Isoelectric Point (pI)

20.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 10 - 102 2.8e-10 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 128
AclWI GGATC 1 cut(s) 184
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 155
AluBI AGCT 3 cut(s) 59, 98, 295
AluI AGCT 3 cut(s) 59, 98, 295
Alw26I GTCTC 2 cut(s) 212, 236
AlwI GGATC 1 cut(s) 184
AlwNI CAGNNNCTG 1 cut(s) 42
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 104, 146
AsuHPI GGTGA 2 cut(s) 77, 227
AvaII GGWCC 1 cut(s) 146
BbvI GCAGC 1 cut(s) 17
BcoDI GTCTC 2 cut(s) 212, 236
BfaI CTAG 1 cut(s) 56
BglII AGATCT 1 cut(s) 70
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 104, 146
BpuEI CTTGAG 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 1 cut(s) 155
BseLI CCNNNNNNNGG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 106
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 212, 236
BsmBI CGTCTC 1 cut(s) 212
BsnI GGCC 1 cut(s) 106
Bsp143I GATC 3 cut(s) 70, 176, 250
BspANI GGCC 1 cut(s) 106
BspFNI CGCG 1 cut(s) 128
BspPI GGATC 1 cut(s) 184
BssMI GATC 3 cut(s) 70, 176, 250
Bst6I CTCTTC 1 cut(s) 24
BstAPI GCANNNNNTGC 1 cut(s) 42
BstBAI YACGTR 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 108
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 3 cut(s) 73, 179, 253
BstMAI GTCTC 2 cut(s) 212, 236
BstMBI GATC 3 cut(s) 70, 176, 250
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstNSI RCATGY 1 cut(s) 280
BstSNI TACGTA 1 cut(s) 27
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 1 cut(s) 106
BtgZI GCGATG 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 108
CaiI CAGNNNCTG 1 cut(s) 42
Cfr13I GGNCC 2 cut(s) 104, 146
CviAII CATG 1 cut(s) 277
CviJI RGCY 4 cut(s) 59, 98, 106, 295
CviKI_1 RGCY 4 cut(s) 59, 98, 106, 295
DpnI GATC 3 cut(s) 72, 178, 252
DpnII GATC 3 cut(s) 70, 176, 250
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Eco105I TACGTA 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 1 cut(s) 280
FaiI YATR 5 cut(s) 116, 183, 237, 267, 278
FatI CATG 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 56
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 1 cut(s) 106
HapII CCGG 1 cut(s) 174
Hin1II CATG 1 cut(s) 280
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HinfI GANTC 1 cut(s) 49
HpaII CCGG 1 cut(s) 174
HphI GGTGA 2 cut(s) 77, 227
Hpy166II GTNNAC 1 cut(s) 303
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 1 cut(s) 303
HpyAV CCTTC 1 cut(s) 266
HpyCH4IV ACGT 3 cut(s) 26, 135, 221
HpyCH4V TGCA 2 cut(s) 36, 204
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpySE526I ACGT 3 cut(s) 26, 135, 221
Hsp92II CATG 1 cut(s) 280
Kzo9I GATC 3 cut(s) 70, 176, 250
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 3 cut(s) 87, 120, 187
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 1 cut(s) 56
MaeII ACGT 3 cut(s) 26, 135, 221
MalI GATC 3 cut(s) 72, 178, 252
MboI GATC 3 cut(s) 70, 176, 250
MboII GAAGA 3 cut(s) 11, 80, 163
MflI RGATCY 1 cut(s) 70
MluCI AATT 1 cut(s) 86
MmeI TCCRAC 2 cut(s) 124, 209
MnlI CCTC 2 cut(s) 153, 255
MseI TTAA 1 cut(s) 255
MspI CCGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 1 cut(s) 42
NdeII GATC 3 cut(s) 70, 176, 250
NlaIII CATG 1 cut(s) 280
NspI RCATGY 1 cut(s) 280
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PfeI GAWTC 1 cut(s) 49
PkrI GCNGC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 27
PspPI GGNCC 2 cut(s) 104, 146
PstNI CAGNNNCTG 1 cut(s) 42
PsuI RGATCY 1 cut(s) 70
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 70, 176, 250
Sau96I GGNCC 2 cut(s) 104, 146
SetI ASST 7 cut(s) 29, 61, 100, 138, 224, 266, 297
SinI GGWCC 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SnaBI TACGTA 1 cut(s) 27
Sse9I AATT 1 cut(s) 86
SspMI CTAG 1 cut(s) 56
TaiI ACGT 3 cut(s) 29, 138, 224
TaqI TCGA 2 cut(s) 249, 288
TaqII GACCGA 1 cut(s) 163
TasI AATT 1 cut(s) 86
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 280
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.