pycom07g26800

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006258
Physical Location & Seq
Reverse (-)
26717619 .. 26718627
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g26800.1

Sequence Viewer

Length: 549 bp
ATGAATAAGTATCAAATTTTGCATTACATTATTTTATTTCCATTATTCCACAAAATTATATTATTATTTTTAGTACTTTTCATCACCCAGCCTTATCTTTTTCATCCCCCTCATCCACTTTTTCCAGCTGAAATACTATTTCCAGTCGACTCTCCAAACTCTACAAATCTGCCCCCTCCTTATGCTTCTTCAATTTTTGGGAGCATAATCTCTTCATACTCCTCCACTTTAATGGCAGAATCCGGCGGCAGTCCCTTCTTCAGCTGCAGAAGTTGCCATAATCCACTTGCTCTTCGTGACGACCTCATCTCCAAGAAATTTCTGGCAAAATCAGGGCCGGCATATATGTTCTCCCATGCAATGAACGTCATTGTGGGACAAAAGGAGCACAGGAAGCTCATAACTGGTGTTTTCTCAGTTGCTGACATCTATTGCAGCAACTGCGGCGAGGTCTTAGGTTGGAAGTACATTCGAGCTTACGAGCAGACTCAGATGTACAAGCAAGGAAAATTTATAATCGAGAGAGCAAAGATTTCTAAGCAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

183

Amino Acids

20.74

Weight (kDa)

9.25

Isoelectric Point (pI)

57.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 87 - 179 1.1e-12 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
AccI GTMKAC 1 cut(s) 147
AciI CCGC 2 cut(s) 246, 444
AcsI RAATTY 3 cut(s) 15, 317, 509
AcuI CTGAAG 1 cut(s) 244
AfaI GTAC 4 cut(s) 75, 467, 497, 545
AgsI TTSAA 1 cut(s) 192
AluBI AGCT 4 cut(s) 128, 264, 397, 476
AluI AGCT 4 cut(s) 128, 264, 397, 476
Alw21I GWGCWC 1 cut(s) 390
AlwNI CAGNNNCTG 2 cut(s) 422, 441
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 264, 435
ApoI RAATTY 3 cut(s) 15, 317, 509
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 2 cut(s) 251, 447
BfaI CTAG 1 cut(s) 547
BfmI CTRYAG 1 cut(s) 265
BisI GCNGC 4 cut(s) 247, 265, 436, 445
BlsI GCNGC 4 cut(s) 248, 266, 437, 446
BmcAI AGTACT 2 cut(s) 75, 545
BmgT120I GGNCC 1 cut(s) 335
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 143, 409
Bse3DI GCAATG 1 cut(s) 366
BseGI GGATG 2 cut(s) 103, 112
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 429, 503
BseNI ACTGG 2 cut(s) 143, 409
BseRI GAGGAG 1 cut(s) 211
BseXI GCAGC 2 cut(s) 251, 447
BseYI CCCAGC 1 cut(s) 87
BshFI GGCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 390
BsiSI CCGG 2 cut(s) 243, 338
BslFI GGGAC 2 cut(s) 237, 390
BsmFI GGGAC 2 cut(s) 237, 390
BsnI GGCC 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 495
BspACI CCGC 2 cut(s) 246, 444
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 428, 502
BspMAI CTGCAG 1 cut(s) 269
BspQI GCTCTTC 1 cut(s) 297
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 1 cut(s) 337
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 2 cut(s) 143, 409
BssAI RCCGGY 1 cut(s) 337
Bst6I CTCTTC 2 cut(s) 217, 297
BstAPI GCANNNNNTGC 2 cut(s) 273, 441
BstAUI TGTACA 1 cut(s) 495
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 4 cut(s) 415, 454, 489, 537
BstF5I GGATG 2 cut(s) 103, 112
BstMWI GCNNNNNNNGC 4 cut(s) 273, 394, 441, 444
BstSFI CTRYAG 1 cut(s) 265
BstV1I GCAGC 2 cut(s) 251, 447
BstXI CCANNNNNNTGG 1 cut(s) 232
BsuRI GGCC 1 cut(s) 337
BtsCI GGATG 2 cut(s) 103, 112
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 2 cut(s) 422, 441
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 4 cut(s) 74, 466, 496, 544
CviAII CATG 1 cut(s) 356
CviJI RGCY 6 cut(s) 91, 128, 264, 337, 397, 476
CviKI_1 RGCY 6 cut(s) 91, 128, 264, 337, 397, 476
CviQI GTAC 4 cut(s) 74, 466, 496, 544
DdeI CTNAG 4 cut(s) 415, 454, 489, 537
Eam1104I CTCTTC 2 cut(s) 217, 297
EarI CTCTTC 2 cut(s) 217, 297
Eco57I CTGAAG 1 cut(s) 244
FaeI CATG 1 cut(s) 359
FaqI GGGAC 2 cut(s) 237, 390
FatI CATG 1 cut(s) 355
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 247, 265, 436, 445
FokI GGATG 2 cut(s) 90, 99
Fsp4HI GCNGC 4 cut(s) 247, 265, 436, 445
FspBI CTAG 1 cut(s) 547
GluI GCNGC 4 cut(s) 247, 265, 436, 445
GsaI CCCAGC 1 cut(s) 91
HaeIII GGCC 1 cut(s) 337
HapII CCGG 2 cut(s) 243, 338
Hin1II CATG 1 cut(s) 359
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 3 cut(s) 149, 239, 487
HpaII CCGG 2 cut(s) 243, 338
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 492
Hpy188III TCNNGA 2 cut(s) 296, 520
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 22, 267, 359, 435
HpyF10VI GCNNNNNNNGC 4 cut(s) 273, 394, 441, 444
HpyF3I CTNAG 4 cut(s) 415, 454, 489, 537
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 1 cut(s) 359
KroI GCCGGC 1 cut(s) 337
KroNI GCCGGC 1 cut(s) 339
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 201, 385
LpnPI CCDG 9 cut(s) 101, 138, 156, 256, 308, 318, 351, 376, 390
Lsp1109I GCAGC 2 cut(s) 251, 447
MaeI CTAG 1 cut(s) 547
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 1 cut(s) 296
MboII GAAGA 4 cut(s) 180, 204, 250, 284
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 5 cut(s) 15, 54, 192, 317, 509
MlyI GAGTC 2 cut(s) 143, 481
MmeI TCCRAC 1 cut(s) 440
MnlI CCTC 5 cut(s) 120, 186, 232, 314, 442
MroNI GCCGGC 1 cut(s) 337
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 2 cut(s) 128, 264
MspI CCGG 2 cut(s) 243, 338
MwoI GCNNNNNNNGC 4 cut(s) 273, 394, 441, 444
NaeI GCCGGC 1 cut(s) 339
NgoMIV GCCGGC 1 cut(s) 337
NlaIII CATG 1 cut(s) 359
NmuCI GTSAC 1 cut(s) 296
PciSI GCTCTTC 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 339
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 4 cut(s) 248, 266, 437, 446
PleI GAGTC 2 cut(s) 143, 481
PpsI GAGTC 2 cut(s) 143, 481
PsiI TTATAA 1 cut(s) 515
PspFI CCCAGC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 335
PstI CTGCAG 1 cut(s) 269
PstNI CAGNNNCTG 2 cut(s) 422, 441
PvuII CAGCTG 2 cut(s) 128, 264
RsaI GTAC 4 cut(s) 75, 467, 497, 545
RsaNI GTAC 4 cut(s) 74, 466, 496, 544
RseI CAYNNNNRTG 1 cut(s) 230
SalI GTCGAC 1 cut(s) 146
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 4 cut(s) 247, 265, 436, 445
Sau96I GGNCC 1 cut(s) 335
ScaI AGTACT 2 cut(s) 75, 545
SchI GAGTC 2 cut(s) 143, 481
SduI GDGCHC 1 cut(s) 390
SetI ASST 8 cut(s) 130, 266, 306, 369, 399, 453, 460, 478
SfcI CTRYAG 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 5 cut(s) 15, 54, 192, 317, 509
SsiI CCGC 2 cut(s) 246, 444
SspMI CTAG 1 cut(s) 547
TaiI ACGT 1 cut(s) 369
TaqI TCGA 3 cut(s) 147, 472, 519
TasI AATT 5 cut(s) 15, 54, 192, 317, 509
TatI WGTACW 4 cut(s) 73, 465, 495, 543
TauI GCSGC 2 cut(s) 249, 447
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 264, 435
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 5 cut(s) 17, 70, 92, 204, 377
XapI RAATTY 3 cut(s) 15, 317, 509
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 1 cut(s) 547
ZrmI AGTACT 2 cut(s) 75, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.