MD01G1224800.v1.1

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006258
Physical Location & Seq
Reverse (-)
31578677 .. 31579812
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1224800.v1.1.491

Sequence Viewer

Length: 363 bp
ATGCCTCCCTCTCCTTATTATTCTTCAATCTTAGGAGCGGATTTAATGGCGGAATCCGGCGGCAACCCCTTCTTCAGCTGCAGAAGTTGCCGTAATCCTCTTGCTCTTCGTGACGACCTTATCTCCAAGAAATTTGTGGGGAAAACTGGGCCGGCATATTTGTTCTCTCATGCAATGAACATCATTGTGGGGCAAAAGGAGGACAGGAAGCTCATATCAGGTGTTTTCTCAGTTGCTGACATCTATTGCAGCAACTGCGGTGAGGTCTTAGGTTGGAAGTACCTTCGAGCTTATGAGCCGACACAGATGTACAAGCAAGGGATGTTCATAATCGAGAGAGCAAAGATTTCTAAGCAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

121

Amino Acids

13.48

Weight (kDa)

9.08

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 25 - 117 4.3e-12 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 38
AciI CCGC 4 cut(s) 38, 50, 60, 258
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 58
AfaI GTAC 3 cut(s) 281, 311, 359
AgsI TTSAA 1 cut(s) 27
AluBI AGCT 3 cut(s) 78, 211, 290
AluI AGCT 3 cut(s) 78, 211, 290
AlwNI CAGNNNCTG 2 cut(s) 236, 255
AoxI GGCC 1 cut(s) 149
ApeKI GCWGC 2 cut(s) 78, 249
ApoI RAATTY 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 272
BbvI GCAGC 2 cut(s) 65, 261
BceAI ACGGC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 79
BisI GCNGC 3 cut(s) 61, 79, 250
BlsI GCNGC 3 cut(s) 62, 80, 251
BmcAI AGTACT 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 149
BmrI ACTGGG 1 cut(s) 156
BmuI ACTGGG 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 180
BseGI GGATG 1 cut(s) 327
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 65, 261
BshFI GGCC 1 cut(s) 151
BsiSI CCGG 2 cut(s) 57, 152
BsnI GGCC 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 309
BspACI CCGC 4 cut(s) 38, 50, 60, 258
BspANI GGCC 1 cut(s) 151
BspCNI CTCAG 1 cut(s) 242
BspMAI CTGCAG 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 111
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 151
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 2 cut(s) 87, 255
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 4 cut(s) 31, 229, 268, 351
BstF5I GGATG 1 cut(s) 327
BstMWI GCNNNNNNNGC 2 cut(s) 87, 255
BstSFI CTRYAG 1 cut(s) 79
BstV1I GCAGC 2 cut(s) 65, 261
BsuRI GGCC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 2 cut(s) 236, 255
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 3 cut(s) 280, 310, 358
CviAII CATG 1 cut(s) 170
CviJI RGCY 5 cut(s) 78, 151, 211, 290, 298
CviKI_1 RGCY 5 cut(s) 78, 151, 211, 290, 298
CviQI GTAC 3 cut(s) 280, 310, 358
DdeI CTNAG 4 cut(s) 31, 229, 268, 351
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 58
FaeI CATG 1 cut(s) 173
FaiI YATR 5 cut(s) 157, 171, 215, 294, 329
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 61, 79, 250
FokI GGATG 1 cut(s) 334
Fsp4HI GCNGC 3 cut(s) 61, 79, 250
GluI GCNGC 3 cut(s) 61, 79, 250
HaeIII GGCC 1 cut(s) 151
HapII CCGG 2 cut(s) 57, 152
Hin1II CATG 1 cut(s) 173
HinfI GANTC 1 cut(s) 53
HpaII CCGG 2 cut(s) 57, 152
HphI GGTGA 1 cut(s) 272
Hpy188III TCNNGA 2 cut(s) 110, 334
HpyAV CCTTC 2 cut(s) 79, 293
HpyCH4V TGCA 3 cut(s) 81, 173, 249
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 255
HpyF3I CTNAG 4 cut(s) 31, 229, 268, 351
Hsp92II CATG 1 cut(s) 173
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 35
LpnPI CCDG 5 cut(s) 70, 132, 165, 190, 204
Lsp1109I GCAGC 2 cut(s) 65, 261
MaeIII GTNAC 1 cut(s) 110
MbiI CCGCTC 1 cut(s) 38
MboII GAAGA 3 cut(s) 15, 64, 98
MluCI AATT 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 5 cut(s) 15, 19, 108, 193, 256
MroNI GCCGGC 1 cut(s) 151
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 2 cut(s) 57, 152
MwoI GCNNNNNNNGC 2 cut(s) 87, 255
NaeI GCCGGC 1 cut(s) 153
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 1 cut(s) 173
NmuCI GTSAC 1 cut(s) 110
PciSI GCTCTTC 1 cut(s) 111
PdiI GCCGGC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 53
PkrI GCNGC 3 cut(s) 62, 80, 251
PspPI GGNCC 1 cut(s) 149
PstI CTGCAG 1 cut(s) 83
PstNI CAGNNNCTG 2 cut(s) 236, 255
PvuII CAGCTG 1 cut(s) 78
RsaI GTAC 3 cut(s) 281, 311, 359
RsaNI GTAC 3 cut(s) 280, 310, 358
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 3 cut(s) 61, 79, 250
Sau96I GGNCC 1 cut(s) 149
ScaI AGTACT 1 cut(s) 359
SetI ASST 8 cut(s) 80, 120, 213, 223, 267, 274, 285, 292
SfcI CTRYAG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 131
SsiI CCGC 4 cut(s) 38, 50, 60, 258
TaqI TCGA 2 cut(s) 286, 333
TasI AATT 1 cut(s) 131
TatI WGTACW 2 cut(s) 309, 357
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 78, 249
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 191, 316
XapI RAATTY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.