AT4G27780

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007027
Physical Location & Seq
Forward (+)
13847549 .. 13849934
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27780.2

Sequence Viewer

Length: 1065 bp
ATGGGTGATTGGGCTCAACTTGCTCAGTCTGTGATCCTAGGTTTGATTTTCTCTTACCTTCTCGCTAAGCTGATCTCGATCGTCGTCACGTTTAAAGAAGACAATCTCTCTCTTACTCGTCACCCAGAGGAGTCTCAATTGGAAATTAAACCGGAAGGAGTTGACTCGCGACGTCTCGATTCCTCTTGCGGTGGTTTCGGTGGTGAGGCGGATTCGCTTGTGGCGGAGCAGGGTAGCTCCCGAAGTGATAGCGTTGCCGGTGACGACAGTGAGGAAGATGATGATTGGGAAGGCGTCGAGAGCACGGAACTCGATGAGGCTTTTAGCGCCGCCACTCTTTTTGTGACTACCGCCGCCGCAGATCGGCTTTCGCAGAAGGTACCGAGTGATGTGCAGCAGCAGCTTTACGGATTGTATAAGATTGCTACGGAAGGGCCGTGTACTGCTCCTCAGCCATCAGCTCTCAAAATGACTGCTCGTGCCAAGTGGCAAGCATGGCAGAAACTGGGAGCTATGCCACCTGAAGAGGCGATGGAGAAGTATATTGAGATTGTCACTCAGCTTTACCCAACTTGGTTAGACGGTGGCGTGAAAGCCGGAAGTCGGGGTGGGGATGATGCAGCCTCCAACTCAAGAGGAACCATGGGACCAGTTTTTAGCTCTTTGGTTTATGATGAGGAGTCCGAAAATGAGTTGAAGATTGATGCCATACACGGATTTGCTAGAGAAGGAGAAGTCGAGAATTTACTGAAAAGCATTGAAAGCGGCATTCCTGTAAATGCAAGGGACAGTGAAGGTCGCACACCATTGCACTGGGCTATAGACCGTGGCCACCTCAACATCGCCAAAGTTCTGGTCGATAAGAACGCCGATGTGAATGCTAAGGACAACGAAGGCCAAACCCCTTTGCATTATGCTGTTGTATGCGACAGAGAAGCTATCGCCGAGTTTCTGGTTAAACAGAACGCAAACACAGCCGCTAAAGATGAGGATGGAAACTCTCCCCTTGATCTCTGTGAATCAGACTGGCCCTGGATCCGAGATTCTGCAAAGCAGGCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000062 GO:0000166 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0005886 GO:0006629 GO:0006810 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009266 GO:0009409 GO:0009505 GO:0009514 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0010029 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0010162 GO:0010288 GO:0010431 GO:0010876 GO:0012505 GO:0016020 GO:0017076 GO:0021700 GO:0022414 GO:0022611 GO:0030312 GO:0030554 GO:0031090 GO:0031903 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032553 GO:0032555 GO:0032559 GO:0032791 GO:0033036 GO:0033218 GO:0033993 GO:0036094 GO:0036293 GO:0042175 GO:0042221 GO:0042579 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0048037 GO:0048316 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050662 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0061458 GO:0065007 GO:0070300 GO:0070482 GO:0071695 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:0098827 GO:1900140 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACBP Ank_2 Ank_4 Ank_5

Protein Analysis

354

Amino Acids

38.48

Weight (kDa)

4.43

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACBP PF00887 105 - 185 7.6e-26 Acyl CoA binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 175
Acc65I GGTACC 1 cut(s) 379
AccB1I GGYRCC 1 cut(s) 379
AccII CGCG 1 cut(s) 169
AciI CCGC 9 cut(s) 189, 209, 224, 330, 351, 354, 357, 765, 978
AclWI GGATC 3 cut(s) 28, 1030, 1043
AcoI YGGCCR 1 cut(s) 829
AcsI RAATTY 1 cut(s) 742
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 2 cut(s) 172, 294
AfaI GTAC 2 cut(s) 381, 442
AfiI CCNNNNNNNGG 2 cut(s) 363, 603
AgsI TTSAA 2 cut(s) 697, 761
AjnI CCWGG 1 cut(s) 1031
AluBI AGCT 8 cut(s) 70, 237, 403, 461, 512, 562, 660, 938
AluI AGCT 8 cut(s) 70, 237, 403, 461, 512, 562, 660, 938
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 2 cut(s) 138, 179
AlwI GGATC 3 cut(s) 28, 1030, 1043
AlwNI CAGNNNCTG 1 cut(s) 505
AoxI GGCC 4 cut(s) 434, 829, 895, 1028
ApeKI GCWGC 4 cut(s) 394, 397, 400, 620
ApoI RAATTY 1 cut(s) 742
ArsI GACNNNNNNTTYG 2 cut(s) 840, 872
Asp718I GGTACC 1 cut(s) 379
AspA2I CCTAGG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 3 cut(s) 434, 647, 1029
AsuHPI GGTGA 4 cut(s) 17, 113, 215, 272
AvaII GGWCC 1 cut(s) 647
AvrII CCTAGG 1 cut(s) 37
BalI TGGCCA 1 cut(s) 831
BamHI GGATCC 1 cut(s) 1035
BanI GGYRCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 16
BauI CACGAG 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 305
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 4 cut(s) 406, 409, 412, 632
BccI CCATC 3 cut(s) 463, 526, 986
BceAI ACGGC 1 cut(s) 421
BcgI CGANNNNNNTGC 6 cut(s) 292, 326, 373, 407, 860, 894
BciT130I CCWGG 1 cut(s) 1033
BcoDI GTCTC 2 cut(s) 138, 179
BfaI CTAG 2 cut(s) 38, 723
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 330
BisI GCNGC 9 cut(s) 330, 354, 357, 395, 398, 401, 621, 766, 978
BlnI CCTAGG 1 cut(s) 37
BlpI GCTNAGC 1 cut(s) 66
BlsI GCNGC 9 cut(s) 331, 355, 358, 396, 399, 402, 622, 767, 979
Bme1390I CCNGG 1 cut(s) 1033
Bme18I GGWCC 1 cut(s) 647
BmgT120I GGNCC 3 cut(s) 434, 647, 1029
BmiI GGNNCC 4 cut(s) 381, 640, 648, 1037
BmrFI CCNGG 1 cut(s) 1033
BmrI ACTGGG 2 cut(s) 515, 823
BmsI GCATC 2 cut(s) 607, 694
BmuI ACTGGG 2 cut(s) 515, 823
BpiI GAAGAC 1 cut(s) 105
Bpu10I CCTNAGC 2 cut(s) 450, 882
Bpu1102I GCTNAGC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 616
BsaBI GATNNNNATC 1 cut(s) 77
BsaHI GRCGYC 2 cut(s) 172, 294
BsaJI CCNNGG 4 cut(s) 37, 642, 826, 1031
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 603
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 4 cut(s) 510, 650, 818, 1031
Bse3DI GCAATG 1 cut(s) 806
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 1 cut(s) 1033
BseDI CCNNGG 4 cut(s) 37, 642, 826, 1031
BseGI GGATG 2 cut(s) 619, 997
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 363, 603
BseMI GCAATG 1 cut(s) 806
BseMII CTCAG 3 cut(s) 38, 464, 572
BseNI ACTGG 4 cut(s) 510, 650, 818, 1031
BseRI GAGGAG 3 cut(s) 143, 438, 692
BseXI GCAGC 4 cut(s) 406, 409, 412, 632
BsgI GTGCAG 1 cut(s) 413
Bsh1236I CGCG 1 cut(s) 169
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 4 cut(s) 436, 831, 897, 1030
BshNI GGYRCC 1 cut(s) 379
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 305
BsiSI CCGG 3 cut(s) 152, 258, 597
BslFI GGGAC 2 cut(s) 660, 800
BslI CCNNNNNNNGG 2 cut(s) 363, 603
BsmAI GTCTC 2 cut(s) 138, 179
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 660, 800
BsmI GAATGC 2 cut(s) 768, 883
BsnI GGCC 4 cut(s) 436, 831, 897, 1030
Bsp1286I GDGCHC 2 cut(s) 16, 305
Bsp143I GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
Bsp1720I GCTNAGC 1 cut(s) 66
Bsp19I CCATGG 1 cut(s) 642
Bsp68I TCGCGA 1 cut(s) 169
BspACI CCGC 9 cut(s) 189, 209, 224, 330, 351, 354, 357, 765, 978
BspANI GGCC 4 cut(s) 436, 831, 897, 1030
BspCNI CTCAG 3 cut(s) 37, 463, 571
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 4 cut(s) 381, 640, 648, 1037
BspPI GGATC 3 cut(s) 28, 1030, 1043
BspT107I GGYRCC 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 806
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 4 cut(s) 510, 650, 818, 1031
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 4 cut(s) 37, 642, 826, 1031
BssMI GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
BssNI GRCGYC 2 cut(s) 172, 294
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 2 cut(s) 37, 642
Bst2BI CACGAG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 1033
Bst4CI ACNGT 4 cut(s) 269, 584, 791, 827
Bst6I CTCTTC 1 cut(s) 519
BstACI GRCGYC 2 cut(s) 172, 294
BstC8I GCNNGC 2 cut(s) 492, 1056
BstDEI CTNAG 5 cut(s) 24, 66, 450, 558, 882
BstDSI CCRYGG 2 cut(s) 642, 826
BstF5I GGATG 2 cut(s) 619, 997
BstFNI CGCG 1 cut(s) 169
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 6 cut(s) 36, 75, 81, 364, 1012, 1038
BstMAI GTCTC 2 cut(s) 138, 179
BstMBI GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 8 cut(s) 20, 300, 326, 400, 496, 762, 974, 1055
BstNI CCWGG 1 cut(s) 1033
BstSCI CCNGG 1 cut(s) 1031
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 169
BstV1I GCAGC 4 cut(s) 406, 409, 412, 632
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 1035
BstXI CCANNNNNNTGG 2 cut(s) 813, 853
BstYI RGATCY 1 cut(s) 1035
BsuRI GGCC 4 cut(s) 436, 831, 897, 1030
BtgI CCRYGG 2 cut(s) 642, 826
BtgZI GCGATG 2 cut(s) 545, 826
BtsCI GGATG 2 cut(s) 619, 997
BtsIMutI CAGTG 3 cut(s) 274, 796, 811
BtuMI TCGCGA 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 492, 1056
CaiI CAGNNNCTG 1 cut(s) 505
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 3 cut(s) 434, 647, 1029
CseI GACGC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 380, 441
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 2 cut(s) 495, 643
CviQI GTAC 2 cut(s) 380, 441
DdeI CTNAG 5 cut(s) 24, 66, 450, 558, 882
DpnI GATC 6 cut(s) 35, 74, 80, 363, 1011, 1037
DpnII GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
DraI TTTAAA 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 829
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
EciI GGCGGA 2 cut(s) 224, 239
Eco130I CCWWGG 2 cut(s) 37, 642
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 647
Eco57I CTGAAG 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 1031
EcoT14I CCWWGG 2 cut(s) 37, 642
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 37, 642
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 2 cut(s) 498, 646
FaqI GGGAC 2 cut(s) 660, 800
FatI CATG 2 cut(s) 494, 642
Fnu4HI GCNGC 9 cut(s) 330, 354, 357, 395, 398, 401, 621, 766, 978
FokI GGATG 2 cut(s) 626, 1004
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 9 cut(s) 330, 354, 357, 395, 398, 401, 621, 766, 978
FspBI CTAG 2 cut(s) 38, 723
GlaI GCGC 1 cut(s) 328
GluI GCNGC 9 cut(s) 330, 354, 357, 395, 398, 401, 621, 766, 978
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 4 cut(s) 436, 831, 897, 1030
HapII CCGG 3 cut(s) 152, 258, 597
HgaI GACGC 1 cut(s) 283
HhaI GCGC 1 cut(s) 329
Hin1I GRCGYC 2 cut(s) 172, 294
Hin1II CATG 2 cut(s) 498, 646
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 7 cut(s) 131, 164, 179, 212, 680, 1019, 1043
HpaII CCGG 3 cut(s) 152, 258, 597
HphI GGTGA 4 cut(s) 17, 113, 215, 272
Hpy166II GTNNAC 2 cut(s) 163, 441
Hpy188I TCNGA 3 cut(s) 685, 1024, 1040
Hpy188III TCNNGA 7 cut(s) 76, 168, 176, 240, 298, 633, 739
Hpy8I GTNNAC 2 cut(s) 163, 441
Hpy99I CGWCG 3 cut(s) 86, 174, 299
HpyAV CCTTC 8 cut(s) 68, 149, 284, 370, 425, 722, 788, 887
HpyCH4III ACNGT 4 cut(s) 269, 584, 791, 827
HpyCH4IV ACGT 2 cut(s) 89, 172
HpyCH4V TGCA 6 cut(s) 394, 620, 782, 811, 910, 1049
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 300, 326, 400, 496, 762, 974, 1055
HpyF3I CTNAG 5 cut(s) 24, 66, 450, 558, 882
HpySE526I ACGT 2 cut(s) 89, 172
Hsp92I GRCGYC 2 cut(s) 172, 294
Hsp92II CATG 2 cut(s) 498, 646
HspAI GCGC 1 cut(s) 327
KpnI GGTACC 1 cut(s) 383
Kzo9I GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
LmnI GCTCC 4 cut(s) 226, 242, 451, 509
Lsp1109I GCAGC 4 cut(s) 406, 409, 412, 632
LweI GCATC 2 cut(s) 607, 694
MaeI CTAG 2 cut(s) 38, 723
MaeII ACGT 2 cut(s) 89, 172
MaeIII GTNAC 5 cut(s) 85, 119, 260, 343, 553
MalI GATC 6 cut(s) 35, 74, 80, 363, 1011, 1037
MboI GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
MboII GAAGA 4 cut(s) 110, 287, 536, 709
MfeI CAATTG 1 cut(s) 137
MflI RGATCY 1 cut(s) 1035
MhlI GDGCHC 2 cut(s) 16, 305
MlsI TGGCCA 1 cut(s) 831
MluCI AATT 3 cut(s) 137, 144, 742
MluNI TGGCCA 1 cut(s) 831
MlyI GAGTC 3 cut(s) 140, 158, 689
MmeI TCCRAC 1 cut(s) 651
Mox20I TGGCCA 1 cut(s) 831
MscI TGGCCA 1 cut(s) 831
MseI TTAA 3 cut(s) 93, 147, 957
Msp20I TGGCCA 1 cut(s) 831
MspI CCGG 3 cut(s) 152, 258, 597
MspR9I CCNGG 1 cut(s) 1033
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 2 cut(s) 768, 883
MvaI CCWGG 1 cut(s) 1033
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 8 cut(s) 20, 300, 326, 400, 496, 762, 974, 1055
NcoI CCATGG 1 cut(s) 642
NdeII GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
NlaIII CATG 2 cut(s) 498, 646
NlaIV GGNNCC 4 cut(s) 381, 640, 648, 1037
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 5 cut(s) 85, 119, 260, 343, 553
NruI TCGCGA 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 434
PctI GAATGC 2 cut(s) 768, 883
PfeI GAWTC 4 cut(s) 179, 212, 1019, 1043
PkrI GCNGC 9 cut(s) 331, 355, 358, 396, 399, 402, 622, 767, 979
Ple19I CGATCG 1 cut(s) 81
PleI GAGTC 3 cut(s) 139, 158, 688
PpsI GAGTC 3 cut(s) 139, 158, 688
Psp6I CCWGG 1 cut(s) 1031
PspGI CCWGG 1 cut(s) 1031
PspN4I GGNNCC 4 cut(s) 381, 640, 648, 1037
PspPI GGNCC 3 cut(s) 434, 647, 1029
PstNI CAGNNNCTG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 1035
PvuI CGATCG 1 cut(s) 81
RruI TCGCGA 1 cut(s) 169
RsaI GTAC 2 cut(s) 381, 442
RsaNI GTAC 2 cut(s) 380, 441
SaqAI TTAA 3 cut(s) 93, 147, 957
SatI GCNGC 9 cut(s) 330, 354, 357, 395, 398, 401, 621, 766, 978
Sau3AI GATC 6 cut(s) 33, 72, 78, 361, 1009, 1035
Sau96I GGNCC 3 cut(s) 434, 647, 1029
SchI GAGTC 3 cut(s) 140, 158, 689
ScrFI CCNGG 1 cut(s) 1033
SduI GDGCHC 2 cut(s) 16, 305
SfaNI GCATC 2 cut(s) 607, 694
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
Sse9I AATT 3 cut(s) 137, 144, 742
SsiI CCGC 9 cut(s) 189, 209, 224, 330, 351, 354, 357, 765, 978
SspMI CTAG 2 cut(s) 38, 723
StyD4I CCNGG 1 cut(s) 1031
StyI CCWWGG 2 cut(s) 37, 642
TaaI ACNGT 4 cut(s) 269, 584, 791, 827
TaiI ACGT 2 cut(s) 92, 175
TaqI TCGA 6 cut(s) 77, 177, 297, 312, 738, 858
TasI AATT 3 cut(s) 137, 144, 742
TatI WGTACW 1 cut(s) 440
TauI GCSGC 5 cut(s) 332, 356, 359, 768, 980
TfiI GAWTC 4 cut(s) 179, 212, 1019, 1043
Tru1I TTAA 3 cut(s) 93, 147, 957
Tru9I TTAA 3 cut(s) 93, 147, 957
TscAI CASTG 3 cut(s) 274, 796, 818
TseFI GTSAC 5 cut(s) 85, 119, 260, 343, 553
TseI GCWGC 4 cut(s) 394, 397, 400, 620
Tsp45I GTSAC 5 cut(s) 85, 119, 260, 343, 553
TspGWI ACGGA 4 cut(s) 320, 423, 443, 729
TspRI CASTG 3 cut(s) 274, 796, 818
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 1 cut(s) 742
XmaJI CCTAGG 1 cut(s) 37
XspI CTAG 2 cut(s) 38, 723
ZraI GACGTC 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.