MD07G1294300.v1.1

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007027
Physical Location & Seq
Reverse (-)
35532436 .. 35535515
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1294300.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGGCGGAGAGTGGCAGCAGCTCCTCCAATCGATAATCCTCGGCCTCATTTTCTCCTACCTCCTCGCCAAGCTCATCGCCATAGTCATCTCCTTCAAGGAAGACAATCTCAGCCTCACTCGCGCTCGAGCTGCCGCCCCCGCCCCCGCCGACACCAATCCGCCGCCGCTCAAACCCGAGCCCCGCGACGACCAGCTTCGCTCCGCCGATTTGAGCTCCGCGGCAATCGAGGCCGAATCGGTCGTCGCCGAGCACGGCAGCGTCAGGAACGAGAGCAGCGAAGCTGACAGTGACGATGACTGGGAAGGCGTGGAGAGTACGGAATTGGACGAGCTGTTCAGCGCGGCGACGGCGTTTGTCGCGGCGGCTGCGGTGGATCGGCAGAAGGTGTCGAGTGATGCGCAGTTGAAGCTTTATGGGCTTTACAAGATTGCTACTGAGGGACCCTGCAGCGCCCCGCAGCCATCTGCTTTGAAAATGACTGCTCGCGCCAAATGGCAAGCTTGGCAGAAATTGGGTGCTATGCCTCCTGAAGATGCTATGGAGAAGTACATTGATATTGTCACAGAGCTTTATCCTAATTGGGCAGCAGGTTTGACTGTGAGCAGAGGTGGAGAAGGTAGTGCATCTGGTAATGATTCTAATGGACCAATGGGACCGGTTTTCAGCACCTTTGTTTACGAGGAAGAATCTGAAAAGGATTTAAAGATGGATGCTATTCATGCCTTTGCCAGAGAAGGAGAAGTGGATAATTTGCTTAAATGCATTGAAAGTGGCGTTTCAGTGGATCAGAAAGATAGTGAGGGAAGGACCCCATTGCATTGGGCTGTGGATCGTGGTCACCTGAACATGACAGAGCTGCTAGTTAGCAAGAATGCCAATGTTAATGAAAAGGACAATGAGAGGCAAACCGCATTGCATTACGCTGTTTTGTGTGACAGAGAAGGCATTGCTGAATTTCTTGTGAAGCAAAATGCAGACACAAGTGCAAAGGACAACGATGGCAACTCTCCCTGTGATGTCTGTGAGTCAAACTGGCCTTGGTTTCAGGGTGCAAAGGAGAAGAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000062 GO:0000166 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0005886 GO:0006629 GO:0006810 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009266 GO:0009409 GO:0009505 GO:0009514 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0010029 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0010162 GO:0010288 GO:0010431 GO:0010876 GO:0012505 GO:0016020 GO:0017076 GO:0021700 GO:0022414 GO:0022611 GO:0030312 GO:0030554 GO:0031090 GO:0031903 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032553 GO:0032555 GO:0032559 GO:0032791 GO:0033036 GO:0033218 GO:0033993 GO:0036094 GO:0036293 GO:0042175 GO:0042221 GO:0042579 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0048037 GO:0048316 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050662 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0061458 GO:0065007 GO:0070300 GO:0070482 GO:0071695 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:0098827 GO:1900140 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACBP Ank_2 Ank_4 Ank_5

Protein Analysis

358

Amino Acids

38.85

Weight (kDa)

4.57

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACBP PF00887 110 - 188 5.6e-27 Acyl CoA binding protein
Ank_4 PF13637 240 - 289 2.2e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 241 - 332 9.3e-18 Ankyrin repeats (3 copies)
Ank PF00023 269 - 300 4.1e-09 Ankyrin repeat
Ank_4 PF13637 273 - 322 6.1e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 292 - 341 5.4e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 402
Acc36I ACCTGC 1 cut(s) 581
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 6 cut(s) 123, 186, 221, 344, 362, 489
AclWI GGATC 3 cut(s) 384, 795, 840
AcsI RAATTY 1 cut(s) 956
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 2 cut(s) 319, 551
AgeI ACCGGT 1 cut(s) 658
AgsI TTSAA 4 cut(s) 97, 409, 475, 770
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
Alw21I GWGCWC 2 cut(s) 218, 255
AlwI GGATC 3 cut(s) 384, 795, 840
Ama87I CYCGRG 2 cut(s) 126, 176
AoxI GGCC 3 cut(s) 43, 231, 1037
ApoI RAATTY 1 cut(s) 956
ArsI GACNNNNNNTTYG 2 cut(s) 228, 260
AsiGI ACCGGT 1 cut(s) 658
AspLEI GCGC 5 cut(s) 125, 344, 403, 455, 491
AspS9I GGNCC 4 cut(s) 443, 647, 656, 810
AsuHPI GGTGA 1 cut(s) 833
AvaI CYCGRG 2 cut(s) 126, 176
AvaII GGWCC 4 cut(s) 443, 647, 656, 810
BanII GRGCYC 2 cut(s) 183, 218
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 218, 255
BccI CCATC 3 cut(s) 472, 703, 995
BceAI ACGGC 2 cut(s) 271, 366
BfaI CTAG 1 cut(s) 863
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 581
Bme18I GGWCC 4 cut(s) 443, 647, 656, 810
BmeT110I CYCGRG 2 cut(s) 126, 176
BmgT120I GGNCC 4 cut(s) 443, 647, 656, 810
BmiI GGNNCC 4 cut(s) 444, 445, 657, 812
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 4 cut(s) 388, 526, 635, 703
BmuI ACTGGG 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 40, 219, 1040
BsaWI WCCGGW 1 cut(s) 658
Bse118I RCCGGY 1 cut(s) 658
Bse1I ACTGG 2 cut(s) 305, 1040
Bse3DI GCAATG 3 cut(s) 815, 914, 948
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 40, 219, 1040
BseGI GGATG 1 cut(s) 718
BseMI GCAATG 3 cut(s) 815, 914, 948
BseMII CTCAG 2 cut(s) 124, 429
BseNI ACTGG 2 cut(s) 305, 1040
BseRI GAGGAG 2 cut(s) 14, 53
Bsh1236I CGCG 6 cut(s) 123, 186, 221, 344, 362, 489
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 3 cut(s) 45, 233, 1039
BshTI ACCGGT 1 cut(s) 658
BshVI ATCGAT 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 243
BsiHKAI GWGCWC 2 cut(s) 218, 255
BsiHKCI CYCGRG 2 cut(s) 126, 176
BsiSI CCGG 1 cut(s) 659
BslFI GGGAC 2 cut(s) 456, 669
BsmFI GGGAC 2 cut(s) 456, 669
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 3 cut(s) 45, 233, 1039
BsoBI CYCGRG 2 cut(s) 126, 176
Bsp1286I GDGCHC 3 cut(s) 183, 218, 255
Bsp143I GATC 3 cut(s) 376, 787, 832
BspANI GGCC 3 cut(s) 45, 233, 1039
BspCNI CTCAG 2 cut(s) 123, 430
BspDI ATCGAT 1 cut(s) 32
BspFNI CGCG 6 cut(s) 123, 186, 221, 344, 362, 489
BspLI GGNNCC 4 cut(s) 444, 445, 657, 812
BspMAI CTGCAG 1 cut(s) 452
BspMI ACCTGC 1 cut(s) 581
BspPI GGATC 3 cut(s) 384, 795, 840
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 3 cut(s) 815, 914, 948
BsrFI RCCGGY 1 cut(s) 658
BsrI ACTGG 2 cut(s) 305, 1040
BssAI RCCGGY 1 cut(s) 658
BssECI CCNNGG 3 cut(s) 40, 219, 1040
BssMI GATC 3 cut(s) 376, 787, 832
BssT1I CCWWGG 1 cut(s) 1040
Bst4CI ACNGT 2 cut(s) 290, 601
Bst6I CTCTTC 1 cut(s) 1058
BstC8I GCNNGC 2 cut(s) 487, 501
BstDEI CTNAG 2 cut(s) 110, 438
BstDSI CCRYGG 1 cut(s) 219
BstEII GGTNACC 1 cut(s) 839
BstF5I GGATG 1 cut(s) 718
BstFNI CGCG 6 cut(s) 123, 186, 221, 344, 362, 489
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 5 cut(s) 125, 344, 403, 455, 491
BstKTI GATC 3 cut(s) 379, 790, 835
BstMBI GATC 3 cut(s) 376, 787, 832
BstMCI CGRYCG 1 cut(s) 243
BstPI GGTNACC 1 cut(s) 839
BstSFI CTRYAG 1 cut(s) 448
BstUI CGCG 6 cut(s) 123, 186, 221, 344, 362, 489
BstV2I GAAGAC 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 822
Bsu15I ATCGAT 1 cut(s) 32
BsuRI GGCC 3 cut(s) 45, 233, 1039
BsuTUI ATCGAT 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 1 cut(s) 718
BtsIMutI CAGTG 3 cut(s) 295, 789, 1069
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 2 cut(s) 487, 501
CfoI GCGC 5 cut(s) 125, 344, 403, 455, 491
Cfr10I RCCGGY 1 cut(s) 658
Cfr13I GGNCC 4 cut(s) 443, 647, 656, 810
Cfr42I CCGCGG 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 32
CseI GACGC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 318, 550
CspAI ACCGGT 1 cut(s) 658
CviAII CATG 2 cut(s) 722, 850
CviQI GTAC 2 cut(s) 318, 550
DdeI CTNAG 2 cut(s) 110, 438
DpnI GATC 3 cut(s) 378, 789, 834
DpnII GATC 3 cut(s) 376, 787, 832
DraI TTTAAA 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 1058
EarI CTCTTC 1 cut(s) 1058
EciI GGCGGA 3 cut(s) 21, 150, 193
Ecl136II GAGCTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 1040
Eco24I GRGCYC 2 cut(s) 183, 218
Eco47I GGWCC 4 cut(s) 443, 647, 656, 810
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 552
Eco88I CYCGRG 2 cut(s) 126, 176
Eco91I GGTNACC 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 443, 810
EcoO65I GGTNACC 1 cut(s) 839
EcoT14I CCWWGG 1 cut(s) 1040
EcoT22I ATGCAT 1 cut(s) 767
EcoT38I GRGCYC 2 cut(s) 183, 218
ErhI CCWWGG 1 cut(s) 1040
FaeI CATG 2 cut(s) 725, 853
FaiI YATR 6 cut(s) 83, 417, 524, 542, 723, 851
FaqI GGGAC 2 cut(s) 456, 669
FatI CATG 2 cut(s) 721, 849
FauI CCCGC 4 cut(s) 148, 154, 191, 465
FokI GGATG 1 cut(s) 725
FriOI GRGCYC 2 cut(s) 183, 218
FspBI CTAG 1 cut(s) 863
FspI TGCGCA 1 cut(s) 402
GlaI GCGC 5 cut(s) 124, 343, 402, 454, 490
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 3 cut(s) 45, 233, 1039
HapII CCGG 1 cut(s) 659
HgaI GACGC 1 cut(s) 250
HhaI GCGC 5 cut(s) 125, 344, 403, 455, 491
Hin1II CATG 2 cut(s) 725, 853
Hin6I GCGC 5 cut(s) 123, 342, 401, 453, 489
HinP1I GCGC 5 cut(s) 123, 342, 401, 453, 489
HindIII AAGCTT 2 cut(s) 410, 501
HinfI GANTC 5 cut(s) 236, 638, 689, 1028, 1066
HpaII CCGG 1 cut(s) 659
HphI GGTGA 1 cut(s) 833
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 2 cut(s) 694, 792
Hpy188III TCNNGA 2 cut(s) 265, 530
Hpy8I GTNNAC 1 cut(s) 679
Hpy99I CGWCG 3 cut(s) 191, 248, 352
HpyAV CCTTC 7 cut(s) 103, 299, 379, 611, 731, 801, 938
HpyCH4III ACNGT 2 cut(s) 290, 601
HpyCH4V TGCA 8 cut(s) 450, 626, 765, 820, 919, 977, 989, 1055
HpyF3I CTNAG 2 cut(s) 110, 438
Hsp92II CATG 2 cut(s) 725, 853
HspAI GCGC 5 cut(s) 123, 342, 401, 453, 489
KflI GGGWCCC 1 cut(s) 443
KspI CCGCGG 1 cut(s) 222
Kzo9I GATC 3 cut(s) 376, 787, 832
LmnI GCTCC 3 cut(s) 27, 206, 221
LweI GCATC 4 cut(s) 388, 526, 635, 703
MaeI CTAG 1 cut(s) 863
MaeIII GTNAC 5 cut(s) 290, 562, 839, 935, 1067
MalI GATC 3 cut(s) 378, 789, 834
MbiI CCGCTC 1 cut(s) 169
MboI GATC 3 cut(s) 376, 787, 832
MboII GAAGA 3 cut(s) 113, 545, 698
MhlI GDGCHC 3 cut(s) 183, 218, 255
MluCI AATT 5 cut(s) 323, 512, 580, 751, 956
MlyI GAGTC 1 cut(s) 1037
Mph1103I ATGCAT 1 cut(s) 767
MseI TTAA 3 cut(s) 704, 759, 885
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 880
MvnI CGCG 6 cut(s) 123, 186, 221, 344, 362, 489
NdeII GATC 3 cut(s) 376, 787, 832
NlaIII CATG 2 cut(s) 725, 853
NlaIV GGNNCC 4 cut(s) 444, 445, 657, 812
NmeAIII GCCGAG 2 cut(s) 21, 274
NmuCI GTSAC 5 cut(s) 290, 562, 839, 935, 1067
NsbI TGCGCA 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 767
PaeR7I CTCGAG 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 3 cut(s) 236, 638, 689
PinAI ACCGGT 1 cut(s) 658
PleI GAGTC 1 cut(s) 1036
PpsI GAGTC 1 cut(s) 1036
PpuMI RGGWCCY 2 cut(s) 443, 810
Psp124BI GAGCTC 1 cut(s) 218
Psp5II RGGWCCY 2 cut(s) 443, 810
PspEI GGTNACC 1 cut(s) 839
PspN4I GGNNCC 4 cut(s) 444, 445, 657, 812
PspPI GGNCC 4 cut(s) 443, 647, 656, 810
PspPPI RGGWCCY 2 cut(s) 443, 810
PspXI VCTCGAGB 1 cut(s) 126
PstI CTGCAG 1 cut(s) 452
RsaI GTAC 2 cut(s) 319, 551
RsaNI GTAC 2 cut(s) 318, 550
SacI GAGCTC 1 cut(s) 218
SacII CCGCGG 1 cut(s) 222
SaqAI TTAA 3 cut(s) 704, 759, 885
Sau3AI GATC 3 cut(s) 376, 787, 832
Sau96I GGNCC 4 cut(s) 443, 647, 656, 810
SchI GAGTC 1 cut(s) 1037
SduI GDGCHC 3 cut(s) 183, 218, 255
SfaNI GCATC 4 cut(s) 388, 526, 635, 703
SfcI CTRYAG 1 cut(s) 448
Sfr274I CTCGAG 1 cut(s) 126
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 4 cut(s) 443, 647, 656, 810
SlaI CTCGAG 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
Sse9I AATT 5 cut(s) 323, 512, 580, 751, 956
SspMI CTAG 1 cut(s) 863
SstI GAGCTC 1 cut(s) 218
StyI CCWWGG 1 cut(s) 1040
TaaI ACNGT 2 cut(s) 290, 601
TaqI TCGA 4 cut(s) 32, 127, 228, 392
TaqII GACCGA 1 cut(s) 229
TasI AATT 5 cut(s) 323, 512, 580, 751, 956
TatI WGTACW 1 cut(s) 549
TauI GCSGC 7 cut(s) 137, 166, 169, 224, 347, 365, 368
TfiI GAWTC 3 cut(s) 236, 638, 689
Tru1I TTAA 3 cut(s) 704, 759, 885
Tru9I TTAA 3 cut(s) 704, 759, 885
TscAI CASTG 2 cut(s) 295, 789
TseFI GTSAC 5 cut(s) 290, 562, 839, 935, 1067
Tsp45I GTSAC 5 cut(s) 290, 562, 839, 935, 1067
TspDTI ATGAA 2 cut(s) 710, 903
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 2 cut(s) 295, 789
VpaK11BI GGWCC 4 cut(s) 443, 647, 656, 810
XapI RAATTY 1 cut(s) 956
XhoI CTCGAG 1 cut(s) 126
XspI CTAG 1 cut(s) 863
Zsp2I ATGCAT 1 cut(s) 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.