AT5G53470

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007027
Physical Location & Seq
Forward (+)
21710170 .. 21712614
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53470.1

Sequence Viewer

Length: 1017 bp
ATGGCTGATTGGTATCAGCTTGCACAGTCTATAATCTTTGGTTTGATCTTCGCTTACCTTCTCGCTAAGCTAATCTCTATCCTCCTCGCTTTTAAAGACGAGAATCTCTCACTCACTCGTAACCACACGACTCAATCGGAGTATGAGAACTTGCGAAAGGTCGAAACTTTAACTGGGATTAGCGGCGAGACTGATTCTCTTATAGCTGAGCAAGGTAGCTTGAGAGGTGATGAAGATGAATCAGATGATGATGATTGGGAAGGTGTCGAAAGTACAGAACTTGATGAGGCATTTAGTGCTGCTACGGCTTTTGTTGCTGCTGCTGCATCGGATAGGCTTTCGCAGAAAGTTTCTAATGAGTTGCAACTTCAGCTTTATGGATTGTATAAAATTGCTACTGAAGGACCTTGTACTGCTCCTCAACCATCAGCTCTTAAAATGACTGCTCGTGCCAAGTGGCAAGCATGGCAGAAATTGGGTGCTATGCCTCCTGAGGAAGCGATGGAGAAGTATATCGACCTTGTTACTCAGCTTTACCCAGCTTGGGTTGAAGGTGGCTCGAAACGAAGAAATCGGAGTGGTGAGGCTGCAGGCCCCATGGGACCGGTTTTTAGCTCATTGGTGTACGAAGAGGAATCTGACAATGAGTTGAAGATTGACGCCATACACGCCTTTGCTAGAGAAGGAGAAGTCGAGAATCTATTGAAATGCATAGAAAATGGCATACCCGTAAATGCAAGAGATAGTGAAGGTCGAACACCACTGCATTGGGCTATAGACCGTGGCCATCTTAACGTTGCTGAAGCTCTGGTTGATAAGAACGCAGATGTGAATGCTAAAGACAATGAAGGCCAAACCTCTCTACACTATGCTGTTGTGTGCGAAAGAGAAGCACTCGCCGAGTTTCTGGTGAAGCAGAAAGCTGATACAACCATTAAAGACGAAGACGGAAACTCGCCTCTTGATCTCTGTGAATCAGAATGGTCTTGGATGCGAGAGAAGAAGGATTCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000062 GO:0000166 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0005886 GO:0006629 GO:0006810 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009266 GO:0009409 GO:0009505 GO:0009514 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0010029 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0010162 GO:0010288 GO:0010431 GO:0010876 GO:0012505 GO:0016020 GO:0017076 GO:0021700 GO:0022414 GO:0022611 GO:0030312 GO:0030554 GO:0031090 GO:0031903 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032553 GO:0032555 GO:0032559 GO:0032791 GO:0033036 GO:0033218 GO:0033993 GO:0036094 GO:0036293 GO:0042175 GO:0042221 GO:0042579 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0048037 GO:0048316 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050662 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0061458 GO:0065007 GO:0070300 GO:0070482 GO:0071695 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:0098827 GO:1900140 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACBP Ank_2 Ank_4 Ank_5

Protein Analysis

338

Amino Acids

37.53

Weight (kDa)

4.52

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACBP PF00887 95 - 175 3.5e-28 Acyl CoA binding protein
Ank_2 PF12796 207 - 281 4.3e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 222 - 271 1e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 223 - 314 4.6e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 238 - 291 1.3e-11 Ankyrin repeats (many copies)
Ank_3 PF13606 250 - 279 8.1e-06 Ankyrin repeat
Ank PF00023 251 - 282 1.8e-09 Ankyrin repeat
Ank_4 PF13637 255 - 304 3.5e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 276 - 324 3.3e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 183
AclI AACGTT 1 cut(s) 795
AcoI YGGCCR 1 cut(s) 784
AcuI CTGAAG 3 cut(s) 353, 420, 822
AcyI GRCGYC 1 cut(s) 660
AfaI GTAC 3 cut(s) 274, 412, 626
AfiI CCNNNNNNNGG 1 cut(s) 544
AgeI ACCGGT 1 cut(s) 604
AgsI TTSAA 3 cut(s) 551, 652, 706
Alw26I GTCTC 1 cut(s) 182
AoxI GGCC 3 cut(s) 592, 784, 850
ApeKI GCWGC 5 cut(s) 299, 317, 320, 323, 587
AsiGI ACCGGT 1 cut(s) 604
AspS9I GGNCC 3 cut(s) 404, 593, 602
AsuHPI GGTGA 3 cut(s) 239, 593, 922
AvaII GGWCC 2 cut(s) 404, 602
AxyI CCTNAGG 1 cut(s) 492
BalI TGGCCA 1 cut(s) 786
BauI CACGAG 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 951
BbvI GCAGC 5 cut(s) 286, 304, 307, 310, 574
BccI CCATC 3 cut(s) 433, 496, 795
BceAI ACGGC 1 cut(s) 321
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 1 cut(s) 678
BfmI CTRYAG 2 cut(s) 588, 774
BisI GCNGC 6 cut(s) 184, 300, 318, 321, 324, 588
BlpI GCTNAGC 2 cut(s) 66, 207
BlsI GCNGC 6 cut(s) 185, 301, 319, 322, 325, 589
Bme18I GGWCC 2 cut(s) 404, 602
BmgT120I GGNCC 3 cut(s) 404, 593, 602
BmiI GGNNCC 2 cut(s) 595, 603
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 335, 981
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 951
BplI GAGNNNNNCTC 4 cut(s) 92, 124, 879, 911
Bpu1102I GCTNAGC 2 cut(s) 66, 207
BpuEI CTTGAG 1 cut(s) 241
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 597, 781
BsaWI WCCGGW 1 cut(s) 604
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse118I RCCGGY 1 cut(s) 604
Bse1I ACTGG 1 cut(s) 178
Bse21I CCTNAGG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 597, 781
BseGI GGATG 1 cut(s) 996
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMII CTCAG 3 cut(s) 198, 483, 542
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 2 cut(s) 74, 408
BseXI GCAGC 5 cut(s) 286, 304, 307, 310, 574
BseYI CCCAGC 1 cut(s) 538
BshFI GGCC 3 cut(s) 594, 786, 852
BshTI ACCGGT 1 cut(s) 604
BsiSI CCGG 1 cut(s) 605
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 615
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 3 cut(s) 594, 786, 852
Bsp143I GATC 2 cut(s) 45, 964
Bsp1720I GCTNAGC 2 cut(s) 66, 207
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 1 cut(s) 183
BspANI GGCC 3 cut(s) 594, 786, 852
BspCNI CTCAG 3 cut(s) 199, 484, 541
BspLI GGNNCC 2 cut(s) 595, 603
BspMAI CTGCAG 1 cut(s) 592
BsrFI RCCGGY 1 cut(s) 604
BsrI ACTGG 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 604
BssECI CCNNGG 2 cut(s) 597, 781
BssMI GATC 2 cut(s) 45, 964
BssNI GRCGYC 1 cut(s) 660
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 597
Bst2BI CACGAG 1 cut(s) 447
Bst4CI ACNGT 2 cut(s) 27, 782
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 1 cut(s) 660
BstAPI GCANNNNNTGC 1 cut(s) 296
BstC8I GCNNGC 3 cut(s) 21, 462, 592
BstDEI CTNAG 4 cut(s) 66, 207, 492, 528
BstDSI CCRYGG 2 cut(s) 597, 781
BstF5I GGATG 1 cut(s) 996
BstKTI GATC 2 cut(s) 48, 967
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 2 cut(s) 45, 964
BstMWI GCNNNNNNNGC 7 cut(s) 296, 305, 314, 323, 370, 466, 668
BstSFI CTRYAG 2 cut(s) 588, 774
BstV1I GCAGC 5 cut(s) 286, 304, 307, 310, 574
BstV2I GAAGAC 1 cut(s) 951
BstXI CCANNNNNNTGG 1 cut(s) 768
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 3 cut(s) 594, 786, 852
BtgI CCRYGG 2 cut(s) 597, 781
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 1 cut(s) 996
BtsI GCAGTG 1 cut(s) 761
BtsIMutI CAGTG 1 cut(s) 761
Cac8I GCNNGC 3 cut(s) 21, 462, 592
Cfr10I RCCGGY 1 cut(s) 604
Cfr13I GGNCC 3 cut(s) 404, 593, 602
CseI GACGC 1 cut(s) 668
Csp6I GTAC 3 cut(s) 273, 411, 625
CspAI ACCGGT 1 cut(s) 604
CviAII CATG 2 cut(s) 465, 598
CviQI GTAC 3 cut(s) 273, 411, 625
DdeI CTNAG 4 cut(s) 66, 207, 492, 528
DpnI GATC 2 cut(s) 47, 966
DpnII GATC 2 cut(s) 45, 964
DraI TTTAAA 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 597
Eco47I GGWCC 2 cut(s) 404, 602
Eco57I CTGAAG 3 cut(s) 353, 420, 822
Eco81I CCTNAGG 1 cut(s) 492
EcoO109I RGGNCCY 2 cut(s) 404, 593
EcoT14I CCWWGG 1 cut(s) 597
EcoT22I ATGCAT 1 cut(s) 713
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 2 cut(s) 468, 601
FaqI GGGAC 1 cut(s) 615
FatI CATG 2 cut(s) 464, 597
Fnu4HI GCNGC 6 cut(s) 184, 300, 318, 321, 324, 588
FokI GGATG 1 cut(s) 1003
Fsp4HI GCNGC 6 cut(s) 184, 300, 318, 321, 324, 588
FspBI CTAG 1 cut(s) 678
GluI GCNGC 6 cut(s) 184, 300, 318, 321, 324, 588
GsaI CCCAGC 1 cut(s) 542
HaeIII GGCC 3 cut(s) 594, 786, 852
HapII CCGG 1 cut(s) 605
HgaI GACGC 1 cut(s) 668
Hin1I GRCGYC 1 cut(s) 660
Hin1II CATG 2 cut(s) 468, 601
HinfI GANTC 8 cut(s) 103, 130, 194, 239, 635, 697, 974, 1007
HpaII CCGG 1 cut(s) 605
HphI GGTGA 3 cut(s) 239, 593, 922
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 6 cut(s) 139, 244, 331, 576, 640, 979
Hpy188III TCNNGA 3 cut(s) 491, 694, 962
Hpy8I GTNNAC 1 cut(s) 625
HpyAV CCTTC 8 cut(s) 68, 254, 395, 545, 677, 743, 842, 997
HpyCH4III ACNGT 2 cut(s) 27, 782
HpyCH4IV ACGT 1 cut(s) 795
HpyCH4V TGCA 7 cut(s) 23, 326, 364, 590, 711, 737, 766
HpyF10VI GCNNNNNNNGC 7 cut(s) 296, 305, 314, 323, 370, 466, 668
HpyF3I CTNAG 4 cut(s) 66, 207, 492, 528
HpySE526I ACGT 1 cut(s) 795
Hsp92I GRCGYC 1 cut(s) 660
Hsp92II CATG 2 cut(s) 468, 601
Kzo9I GATC 2 cut(s) 45, 964
LmnI GCTCC 1 cut(s) 421
LpnPI CCDG 7 cut(s) 159, 504, 552, 576, 618, 794, 893
Lsp1109I GCAGC 5 cut(s) 286, 304, 307, 310, 574
LweI GCATC 2 cut(s) 335, 981
MaeI CTAG 1 cut(s) 678
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 2 cut(s) 119, 523
MalI GATC 2 cut(s) 47, 966
MboI GATC 2 cut(s) 45, 964
MboII GAAGA 7 cut(s) 40, 245, 579, 641, 664, 956, 1012
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 3 cut(s) 390, 473, 1012
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 1 cut(s) 124
Mox20I TGGCCA 1 cut(s) 786
Mph1103I ATGCAT 1 cut(s) 713
MscI TGGCCA 1 cut(s) 786
MseI TTAA 5 cut(s) 93, 170, 435, 792, 936
Msp20I TGGCCA 1 cut(s) 786
MspI CCGG 1 cut(s) 605
Mva1269I GAATGC 1 cut(s) 838
MwoI GCNNNNNNNGC 7 cut(s) 296, 305, 314, 323, 370, 466, 668
NcoI CCATGG 1 cut(s) 597
NdeII GATC 2 cut(s) 45, 964
NlaIII CATG 2 cut(s) 468, 601
NlaIV GGNNCC 2 cut(s) 595, 603
NmeAIII GCCGAG 1 cut(s) 925
NsiI ATGCAT 1 cut(s) 713
PctI GAATGC 1 cut(s) 838
PfeI GAWTC 7 cut(s) 103, 194, 239, 635, 697, 974, 1007
PinAI ACCGGT 1 cut(s) 604
PkrI GCNGC 6 cut(s) 185, 301, 319, 322, 325, 589
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
PpuMI RGGWCCY 1 cut(s) 404
Psp1406I AACGTT 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 538
PspN4I GGNNCC 2 cut(s) 595, 603
PspPI GGNCC 3 cut(s) 404, 593, 602
PspPPI RGGWCCY 1 cut(s) 404
PstI CTGCAG 1 cut(s) 592
RsaI GTAC 3 cut(s) 274, 412, 626
RsaNI GTAC 3 cut(s) 273, 411, 625
SaqAI TTAA 5 cut(s) 93, 170, 435, 792, 936
SatI GCNGC 6 cut(s) 184, 300, 318, 321, 324, 588
Sau3AI GATC 2 cut(s) 45, 964
Sau96I GGNCC 3 cut(s) 404, 593, 602
SchI GAGTC 1 cut(s) 124
SfaNI GCATC 2 cut(s) 335, 981
SfcI CTRYAG 2 cut(s) 588, 774
SinI GGWCC 2 cut(s) 404, 602
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 3 cut(s) 390, 473, 1012
SsiI CCGC 1 cut(s) 183
SspMI CTAG 1 cut(s) 678
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 2 cut(s) 27, 782
TaiI ACGT 1 cut(s) 798
TaqI TCGA 6 cut(s) 162, 267, 516, 560, 693, 754
TasI AATT 3 cut(s) 390, 473, 1012
TatI WGTACW 2 cut(s) 272, 410
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 7 cut(s) 103, 194, 239, 635, 697, 974, 1007
Tru1I TTAA 5 cut(s) 93, 170, 435, 792, 936
Tru9I TTAA 5 cut(s) 93, 170, 435, 792, 936
TscAI CASTG 1 cut(s) 768
TseI GCWGC 5 cut(s) 299, 317, 320, 323, 587
TspDTI ATGAA 3 cut(s) 246, 252, 861
TspGWI ACGGA 1 cut(s) 963
TspRI CASTG 1 cut(s) 768
VpaK11BI GGWCC 2 cut(s) 404, 602
XspI CTAG 1 cut(s) 678
Zsp2I ATGCAT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.