MD02G1171000.v1.1

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007027
Physical Location & Seq
Forward (+)
14824206 .. 14824643
438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1171000.v1.1.491

Sequence Viewer

Length: 438 bp
ATGAACAAGGGCAGTGAATGTGACAGTGATGATGACTGGGAAGGGGTGGAAAGTACGGAATTGGACAAGCTGTTCAACGCGGTGTCGGCATTTGTCGCGGCGGCCGTGACGGATCAGCAAAAGGTGTTGACTGATGTGCAGTTGAAGCTTTATGGGCTTTACAAGATTGCTACTAAGGGTCCCTGCAGCGTGTCGCAGCCATCGACTCTGAAAATAACTGCTTGCACCAAATGGCAAGCTTGGCAAAAATTGGGTGCTATGCCTCCTGAAAATGCTATGGAGAAGTACATTGATATTGTCACATTGCTTTATCCTAATTGGACAGCAGGTTTGATTATGAAAAGCAGAGGAGGTGGAGATGGTAGTGCACTTGGTAATGATTCTAAAGGACCAATGAGACCGGTTTTCAACACCTTTGTTTACGAGGAAGAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000062 GO:0000166 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0005886 GO:0006629 GO:0006810 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009266 GO:0009409 GO:0009505 GO:0009514 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0010029 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0010162 GO:0010288 GO:0010431 GO:0010876 GO:0012505 GO:0016020 GO:0017076 GO:0021700 GO:0022414 GO:0022611 GO:0030312 GO:0030554 GO:0031090 GO:0031903 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032553 GO:0032555 GO:0032559 GO:0032791 GO:0033036 GO:0033218 GO:0033993 GO:0036094 GO:0036293 GO:0042175 GO:0042221 GO:0042579 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0048037 GO:0048316 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050662 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0061458 GO:0065007 GO:0070300 GO:0070482 GO:0071695 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:0098827 GO:1900140 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACBP Ank_2 Ank_4 Ank_5

Protein Analysis

146

Amino Acids

15.85

Weight (kDa)

4.72

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACBP PF00887 23 - 100 8.1e-23 Acyl CoA binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 317
AccII CGCG 2 cut(s) 80, 98
AciI CCGC 3 cut(s) 80, 98, 101
AclWI GGATC 1 cut(s) 120
AcoI YGGCCR 1 cut(s) 102
AfaI GTAC 2 cut(s) 55, 287
AgeI ACCGGT 1 cut(s) 400
AgsI TTSAA 3 cut(s) 76, 145, 409
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 70, 148, 239
AluI AGCT 3 cut(s) 70, 148, 239
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 1 cut(s) 391
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 1 cut(s) 120
AoxI GGCC 1 cut(s) 102
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 2 cut(s) 186, 196
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
AsiGI ACCGGT 1 cut(s) 400
AspS9I GGNCC 2 cut(s) 179, 389
AvaII GGWCC 2 cut(s) 179, 389
BaeGI GKGCMC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 198, 208
BccI CCATC 2 cut(s) 208, 353
BceAI ACGGC 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 391
BfmI CTRYAG 1 cut(s) 184
BfuAI ACCTGC 1 cut(s) 317
BisI GCNGC 4 cut(s) 99, 102, 187, 197
BlsI GCNGC 4 cut(s) 100, 103, 188, 198
Bme18I GGWCC 2 cut(s) 179, 389
BmgT120I GGNCC 2 cut(s) 179, 389
BmiI GGNNCC 2 cut(s) 180, 181
BmrI ACTGGG 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 391
BsaWI WCCGGW 1 cut(s) 400
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 1 cut(s) 41
Bse3DI GCAATG 1 cut(s) 302
BseMI GCAATG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 41
BseRI GAGGAG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 370
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 198, 208
BsgI GTGCAG 1 cut(s) 158
Bsh1236I CGCG 2 cut(s) 80, 98
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 104
BshTI ACCGGT 1 cut(s) 400
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 165
BsmAI GTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 165
BsnI GGCC 1 cut(s) 104
Bso31I GGTCTC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 370
Bsp143I GATC 1 cut(s) 112
BspACI CCGC 3 cut(s) 80, 98, 101
BspANI GGCC 1 cut(s) 104
BspFNI CGCG 2 cut(s) 80, 98
BspLI GGNNCC 2 cut(s) 180, 181
BspMAI CTGCAG 1 cut(s) 188
BspMI ACCTGC 1 cut(s) 317
BspPI GGATC 1 cut(s) 120
BspTNI GGTCTC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 1 cut(s) 41
BssAI RCCGGY 1 cut(s) 400
BssMI GATC 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 223, 237
BstDEI CTNAG 1 cut(s) 174
BstFNI CGCG 2 cut(s) 80, 98
BstKTI GATC 1 cut(s) 115
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 1 cut(s) 112
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 5 cut(s) 86, 95, 145, 154, 241
BstSFI CTRYAG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 370
BstUI CGCG 2 cut(s) 80, 98
BstV1I GCAGC 2 cut(s) 198, 208
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 1 cut(s) 104
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 2 cut(s) 19, 31
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 2 cut(s) 223, 237
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 179, 389
Csp6I GTAC 2 cut(s) 54, 286
CspAI ACCGGT 1 cut(s) 400
CviJI RGCY 6 cut(s) 70, 104, 148, 157, 199, 239
CviKI_1 RGCY 6 cut(s) 70, 104, 148, 157, 199, 239
CviQI GTAC 2 cut(s) 54, 286
DdeI CTNAG 1 cut(s) 174
DpnI GATC 1 cut(s) 114
DpnII GATC 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
EclXI CGGCCG 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 179, 389
Eco52I CGGCCG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 179
FaiI YATR 4 cut(s) 153, 260, 278, 338
FaqI GGGAC 1 cut(s) 165
Fnu4HI GCNGC 4 cut(s) 99, 102, 187, 197
Fsp4HI GCNGC 4 cut(s) 99, 102, 187, 197
GluI GCNGC 4 cut(s) 99, 102, 187, 197
HaeIII GGCC 1 cut(s) 104
HapII CCGG 1 cut(s) 401
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 2 cut(s) 146, 237
HinfI GANTC 3 cut(s) 205, 380, 431
HpaII CCGG 1 cut(s) 401
Hpy166II GTNNAC 3 cut(s) 129, 368, 421
Hpy188I TCNGA 1 cut(s) 210
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 3 cut(s) 129, 368, 421
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4V TGCA 4 cut(s) 139, 186, 225, 368
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 95, 145, 154, 241
HpyF3I CTNAG 1 cut(s) 174
KflI GGGWCCC 1 cut(s) 179
Kzo9I GATC 1 cut(s) 112
LpnPI CCDG 5 cut(s) 22, 196, 279, 312, 414
Lsp1109I GCAGC 2 cut(s) 198, 208
MaeIII GTNAC 3 cut(s) 20, 106, 298
MalI GATC 1 cut(s) 114
MboI GATC 1 cut(s) 112
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 3 cut(s) 59, 248, 316
MlyI GAGTC 1 cut(s) 199
MnlI CCTC 4 cut(s) 273, 341, 344, 418
MseI TTAA 1 cut(s) 436
MspI CCGG 1 cut(s) 401
MvnI CGCG 2 cut(s) 80, 98
MwoI GCNNNNNNNGC 5 cut(s) 86, 95, 145, 154, 241
NdeII GATC 1 cut(s) 112
NlaIV GGNNCC 2 cut(s) 180, 181
NmuCI GTSAC 3 cut(s) 20, 106, 298
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 200
PfeI GAWTC 2 cut(s) 380, 431
PinAI ACCGGT 1 cut(s) 400
PkrI GCNGC 4 cut(s) 100, 103, 188, 198
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PpuMI RGGWCCY 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 180, 181
PspPI GGNCC 2 cut(s) 179, 389
PspPPI RGGWCCY 1 cut(s) 179
PstI CTGCAG 1 cut(s) 188
RsaI GTAC 2 cut(s) 55, 287
RsaNI GTAC 2 cut(s) 54, 286
SaqAI TTAA 1 cut(s) 436
SatI GCNGC 4 cut(s) 99, 102, 187, 197
Sau3AI GATC 1 cut(s) 112
Sau96I GGNCC 2 cut(s) 179, 389
SchI GAGTC 1 cut(s) 199
SduI GDGCHC 1 cut(s) 370
SetI ASST 7 cut(s) 72, 126, 150, 241, 331, 355, 416
SfcI CTRYAG 1 cut(s) 184
SinI GGWCC 2 cut(s) 179, 389
Sse9I AATT 3 cut(s) 59, 248, 316
SsiI CCGC 3 cut(s) 80, 98, 101
TaaI ACNGT 1 cut(s) 26
TaqI TCGA 1 cut(s) 203
TasI AATT 3 cut(s) 59, 248, 316
TatI WGTACW 1 cut(s) 285
TauI GCSGC 2 cut(s) 101, 104
TfiI GAWTC 2 cut(s) 380, 431
Tru1I TTAA 1 cut(s) 436
Tru9I TTAA 1 cut(s) 436
TscAI CASTG 2 cut(s) 19, 31
TseFI GTSAC 3 cut(s) 20, 106, 298
TseI GCWGC 2 cut(s) 186, 196
Tsp45I GTSAC 3 cut(s) 20, 106, 298
TspDTI ATGAA 2 cut(s) 17, 353
TspGWI ACGGA 2 cut(s) 71, 125
TspRI CASTG 2 cut(s) 19, 31
VneI GTGCAC 1 cut(s) 366
VpaK11BI GGWCC 2 cut(s) 179, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.