AT4G27790

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013165
Physical Location & Seq
Forward (+)
13849949 .. 13851973
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27790.2

Sequence Viewer

Length: 1038 bp
ATGGCCAAGGTGGTGGTTTACACGATACTAACCGCCACCATCATCTTCCTCATCCTCCTCGCTCATAAGAAACAAAACCAGACTCAATCCATTGAGGGTCTCATAACCCGCCGCATCGGACGGAGGCTTGAAATGCCGGTGTTTGACCCTTTGGTGACCCGGATTGAGAGGTTGTCTCATGAGAAAGAAGCTGGTACGAAGACCGTGGAGGCTGCGAAAGAGGAGAAAGATGATATGTTTGAGGAGTATTTTGCACAAGAGAGAAGGCTTAACACGACAATGAGGATCAAGTTCTTGTTTCCTCTGCTTGACGCGTCTCCAAGAGATGGCTTTGTGAGCTTGAAAGAGCTTCAGACATGGATGATGCAACAGACAGAAGACAACATGGTTTACAGAACCGCCAAAGAGCTTGAGCTACAAGATAAGGACAAGGACGGTGTCATAACTTTCGAAGAGTACTTGCCTCAATTCTCTAAACAAGACATTGAGAAAAACGAGAAGGGTCACGGTGAAGCCGGTTGGTGGATGGAGCAATTCAAGAATTCTGATTTTGATCATAATGGCTCTCTCGACATTGAGGAGTTCAACAATTTCTTGCACCCTGAAGACAGCAGAAACGGAGATACACAAAGATGGGTTCTAAAGGAGCGAATGACGGGCATGGACACGAACGGTGATGGGAAGCTAGAGTACAAAGAGTTTGTAAAAAATGCATATGAAATGTATAAAGAATTCGCAAAGTTCGAGAAAGAGGAGGATGAGAATGTGCCAACACCTCAGCTTCTGTTCGCAGAGATGGACAGAGACAAAGACAGATTCCTCGTGGCCGATGAGCTTCGACCCATTTTGCAATATCTTCAACCTGGTGAGATGAGTTATGCCAAATTCTATTCTACTTTCCTTTGCCACGAGGCGGATGAGGATAAAGATGGTAAACTATCTCTTGAAGAGATGTTGCACCACGAAGATGTCTTTTACAAGGCAGTTCATCACGAAGACTTGGACGACGAAGACTATTTTGATCATGATGAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

345

Amino Acids

40.91

Weight (kDa)

4.73

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 95 - 154 9.4e-06 EF-hand domain pair
EF-hand_7 PF13499 175 - 237 8.1e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 326
AccII CGCG 1 cut(s) 314
AciI CCGC 5 cut(s) 33, 109, 112, 399, 914
AclWI GGATC 1 cut(s) 293
AcoI YGGCCR 2 cut(s) 3, 825
AcsI RAATTY 3 cut(s) 541, 731, 884
AcuI CTGAAG 2 cut(s) 335, 624
AfaI GTAC 3 cut(s) 196, 458, 692
AfiI CCNNNNNNNGG 3 cut(s) 326, 522, 913
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 6 cut(s) 131, 343, 538, 586, 860, 947
AjnI CCWGG 1 cut(s) 862
AluBI AGCT 8 cut(s) 191, 339, 349, 409, 415, 685, 781, 835
AluI AGCT 8 cut(s) 191, 339, 349, 409, 415, 685, 781, 835
Alw26I GTCTC 4 cut(s) 104, 180, 321, 798
AlwI GGATC 1 cut(s) 293
AlwNI CAGNNNCTG 1 cut(s) 784
AoxI GGCC 2 cut(s) 3, 825
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 3 cut(s) 541, 731, 884
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 4 cut(s) 166, 521, 686, 878
AsuII TTCGAA 1 cut(s) 450
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 674, 706
BauI CACGAG 2 cut(s) 821, 908
BbsI GAAGAC 5 cut(s) 206, 384, 612, 1002, 1017
BbvCI CCTCAGC 1 cut(s) 777
BbvI GCAGC 1 cut(s) 199
BccI CCATC 7 cut(s) 47, 320, 520, 627, 671, 790, 923
BciT130I CCWGG 1 cut(s) 864
BclI TGATCA 2 cut(s) 553, 1021
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 4 cut(s) 104, 180, 321, 798
BfaI CTAG 1 cut(s) 686
BisI GCNGC 2 cut(s) 112, 213
BlsI GCNGC 2 cut(s) 113, 214
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 160, 864
BmrFI CCNGG 2 cut(s) 160, 864
BmsI GCATC 2 cut(s) 123, 354
BpiI GAAGAC 5 cut(s) 206, 384, 612, 1002, 1017
BplI GAGNNNNNCTC 2 cut(s) 160, 192
Bpu10I CCTNAGC 1 cut(s) 777
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 6, 204
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 522, 913
Bse118I RCCGGY 2 cut(s) 136, 515
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 1 cut(s) 864
BseDI CCNNGG 2 cut(s) 6, 204
BseGI GGATG 5 cut(s) 51, 366, 531, 763, 922
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 3 cut(s) 326, 522, 913
BseMII CTCAG 1 cut(s) 791
BseRI GAGGAG 5 cut(s) 47, 236, 257, 593, 767
BseXI GCAGC 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 314
BshFI GGCC 2 cut(s) 5, 827
BsiSI CCGG 3 cut(s) 137, 160, 516
BslI CCNNNNNNNGG 3 cut(s) 326, 522, 913
BsmAI GTCTC 4 cut(s) 104, 180, 321, 798
BsmBI CGTCTC 1 cut(s) 321
BsnI GGCC 2 cut(s) 5, 827
Bso31I GGTCTC 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 450
Bsp143I GATC 3 cut(s) 285, 553, 1021
BspACI CCGC 5 cut(s) 33, 109, 112, 399, 914
BspANI GGCC 2 cut(s) 5, 827
BspCNI CTCAG 1 cut(s) 790
BspFNI CGCG 1 cut(s) 314
BspHI TCATGA 2 cut(s) 178, 1024
BspPI GGATC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 450
BspTNI GGTCTC 1 cut(s) 104
BsrFI RCCGGY 2 cut(s) 136, 515
BssAI RCCGGY 2 cut(s) 136, 515
BssECI CCNNGG 2 cut(s) 6, 204
BssMI GATC 3 cut(s) 285, 553, 1021
BssSI CACGAG 2 cut(s) 821, 908
BssT1I CCWWGG 1 cut(s) 6
Bst2BI CACGAG 2 cut(s) 821, 908
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 4 cut(s) 205, 437, 509, 674
Bst6I CTCTTC 2 cut(s) 447, 942
BstBI TTCGAA 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 777
BstDSI CCRYGG 1 cut(s) 204
BstEII GGTNACC 1 cut(s) 154
BstF5I GGATG 5 cut(s) 51, 366, 531, 763, 922
BstFNI CGCG 1 cut(s) 314
BstKTI GATC 3 cut(s) 288, 556, 1024
BstMAI GTCTC 4 cut(s) 104, 180, 321, 798
BstMBI GATC 3 cut(s) 285, 553, 1021
BstMWI GCNNNNNNNGC 2 cut(s) 133, 336
BstNI CCWGG 1 cut(s) 864
BstPI GGTNACC 1 cut(s) 154
BstSCI CCNGG 2 cut(s) 158, 862
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 5 cut(s) 206, 384, 612, 1002, 1017
BstXI CCANNNNNNTGG 1 cut(s) 13
BsuRI GGCC 2 cut(s) 5, 827
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 5 cut(s) 51, 366, 531, 763, 922
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 2 cut(s) 178, 1024
Cfr10I RCCGGY 2 cut(s) 136, 515
CseI GACGC 2 cut(s) 303, 320
CsiI ACCWGGT 1 cut(s) 862
Csp6I GTAC 3 cut(s) 195, 457, 691
CviAII CATG 5 cut(s) 179, 357, 385, 661, 1025
CviQI GTAC 3 cut(s) 195, 457, 691
DdeI CTNAG 1 cut(s) 777
DpnI GATC 3 cut(s) 287, 555, 1023
DpnII GATC 3 cut(s) 285, 553, 1021
EaeI YGGCCR 2 cut(s) 3, 825
Eam1104I CTCTTC 2 cut(s) 447, 942
EarI CTCTTC 2 cut(s) 447, 942
EciI GGCGGA 1 cut(s) 929
Eco130I CCWWGG 1 cut(s) 6
Eco31I GGTCTC 1 cut(s) 104
Eco57I CTGAAG 2 cut(s) 335, 624
Eco91I GGTNACC 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 154
EcoRI GAATTC 2 cut(s) 541, 731
EcoRII CCWGG 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 715
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 321
FaeI CATG 5 cut(s) 182, 360, 388, 664, 1028
FatI CATG 5 cut(s) 178, 356, 384, 660, 1024
FauI CCCGC 1 cut(s) 116
FauNDI CATATG 1 cut(s) 715
FbaI TGATCA 2 cut(s) 553, 1021
Fnu4HI GCNGC 2 cut(s) 112, 213
FokI GGATG 5 cut(s) 38, 373, 538, 770, 929
Fsp4HI GCNGC 2 cut(s) 112, 213
FspBI CTAG 1 cut(s) 686
GluI GCNGC 2 cut(s) 112, 213
HaeIII GGCC 2 cut(s) 5, 827
HapII CCGG 3 cut(s) 137, 160, 516
HgaI GACGC 2 cut(s) 303, 320
Hin1II CATG 5 cut(s) 182, 360, 388, 664, 1028
HinfI GANTC 2 cut(s) 82, 816
HpaII CCGG 3 cut(s) 137, 160, 516
HphI GGTGA 4 cut(s) 166, 521, 686, 878
Hpy166II GTNNAC 3 cut(s) 19, 391, 935
Hpy188I TCNGA 3 cut(s) 119, 354, 547
Hpy188III TCNNGA 7 cut(s) 179, 538, 569, 745, 944, 992, 1025
Hpy8I GTNNAC 3 cut(s) 19, 391, 935
Hpy99I CGWCG 1 cut(s) 1010
HpyAV CCTTC 2 cut(s) 258, 493
HpyCH4III ACNGT 4 cut(s) 205, 437, 509, 674
HpyCH4V TGCA 6 cut(s) 254, 367, 598, 713, 850, 958
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 336
HpyF3I CTNAG 1 cut(s) 777
Hsp92II CATG 5 cut(s) 182, 360, 388, 664, 1028
Ksp22I TGATCA 2 cut(s) 553, 1021
Kzo9I GATC 3 cut(s) 285, 553, 1021
LmnI GCTCC 2 cut(s) 529, 646
LpnPI CCDG 8 cut(s) 92, 150, 173, 177, 529, 615, 849, 876
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 2 cut(s) 123, 354
MabI ACCWGGT 1 cut(s) 862
MaeI CTAG 1 cut(s) 686
MaeIII GTNAC 2 cut(s) 154, 503
MalI GATC 3 cut(s) 287, 555, 1023
MboI GATC 3 cut(s) 285, 553, 1021
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 467, 533, 541, 589, 731, 884
MluI ACGCGT 1 cut(s) 312
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 76
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 715
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 270
MslI CAYNNNNRTG 3 cut(s) 278, 631, 966
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 137, 160, 516
MspR9I CCNGG 2 cut(s) 160, 864
MvaI CCWGG 1 cut(s) 864
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 133, 336
NciI CCSGG 1 cut(s) 160
NdeI CATATG 1 cut(s) 715
NdeII GATC 3 cut(s) 285, 553, 1021
NlaIII CATG 5 cut(s) 182, 360, 388, 664, 1028
NmuCI GTSAC 2 cut(s) 154, 503
NsiI ATGCAT 1 cut(s) 715
NspV TTCGAA 1 cut(s) 450
PagI TCATGA 2 cut(s) 178, 1024
PcsI WCGNNNNNNNCGW 1 cut(s) 741
PfeI GAWTC 1 cut(s) 816
PflFI GACNNNGTC 1 cut(s) 437
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 2 cut(s) 113, 214
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 862
PspEI GGTNACC 1 cut(s) 154
PspGI CCWGG 1 cut(s) 862
PstNI CAGNNNCTG 1 cut(s) 784
PsyI GACNNNGTC 1 cut(s) 437
RsaI GTAC 3 cut(s) 196, 458, 692
RsaNI GTAC 3 cut(s) 195, 457, 691
RseI CAYNNNNRTG 3 cut(s) 278, 631, 966
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 2 cut(s) 112, 213
Sau3AI GATC 3 cut(s) 285, 553, 1021
ScaI AGTACT 1 cut(s) 458
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 2 cut(s) 160, 864
SexAI ACCWGGT 1 cut(s) 862
SfaNI GCATC 2 cut(s) 123, 354
SfuI TTCGAA 1 cut(s) 450
SmiMI CAYNNNNRTG 3 cut(s) 278, 631, 966
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 6 cut(s) 467, 533, 541, 589, 731, 884
SsiI CCGC 5 cut(s) 33, 109, 112, 399, 914
SspMI CTAG 1 cut(s) 686
StyD4I CCNGG 2 cut(s) 158, 862
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 4 cut(s) 205, 437, 509, 674
TaqI TCGA 4 cut(s) 450, 570, 744, 838
TasI AATT 6 cut(s) 467, 533, 541, 589, 731, 884
TatI WGTACW 2 cut(s) 456, 690
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 1 cut(s) 816
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TseFI GTSAC 2 cut(s) 154, 503
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 2 cut(s) 154, 503
TspDTI ATGAA 2 cut(s) 732, 977
TspGWI ACGGA 2 cut(s) 136, 633
Tth111I GACNNNGTC 1 cut(s) 437
Van91I CCANNNNNTGG 1 cut(s) 326
XapI RAATTY 3 cut(s) 541, 731, 884
XspI CTAG 1 cut(s) 686
ZrmI AGTACT 1 cut(s) 458
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.