pycom07g26700

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013165
Physical Location & Seq
Reverse (-)
26659366 .. 26661150
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g26700.3

Sequence Viewer

Length: 1185 bp
ATGGCGAAGGCAGTGGTCTACGCGCTACTTGCCACCGCCTTTATCTTTCTCCTCGTCATGCTTCCCTCCAAACACCGTGGCCCCAACCACCCCCACCCCCACCTTGGCCTCAACCGACGTCTTGGCTACGCGGGTCACGCCCCCATTTTCGACCCTCTGGCAGCCAAGATGGAGTGGTACGCCAAGGAAAGTGGTGGATTCAGAGGGGAAACGAATAGTCGCGTGGGGTTGTCGGAACACAACAACAATCCCATGATGAATGCGAGTGAGGTGGAGGAAATGCATGAATATCTCGGCGATGAGGGAAAGTTGAACCTCACGTTGAGGCTGATTACTTTGTTCCCGTTGATAGACCAGGCGCCAAAAGACGGGTTGATTAACTCTAATGAGCTCGAGGATTGGGTCATGAAACAAACCGTGGAGAGGTTGAATTATAGGACGCAGAAAGAAGTGGAATCGTATGACAAAGATGGTGATGGAACAATTTCTTTCATGGAGTATCTGCCCAAGTTTTCCATACAGGACCTAGTAAATTCCCTTCATTCCCATATTACTGTGGCAAAAAATATACGATTTGCGAGTTCTGGTTCAACAGAAATCTCCATTTTTTGTTGCAGTTTGTTGCACCCAGAAGACAGTAACAACGAAGAAATTCACAAATGGCTGTTGAGGGAAAAAATGAAGTACATTTTCTCACATATCTTCTCTGTCTTTCACACTAAACATATTAATGCCTTAATATATTTTCTGAATAATTTGATGCAAAGGAACGGATTAACGAAATGTCTGCTTATAATTTACAGGCGGATGGATCAGGATAACGATGGCAAACTGAATTTCCACGAGTTTTTACACAACGCTTATGATAATTACAAGACTTACGTTGAATTTGAAAGGGGCGGGAAAAATGTACCCACACCGGAAGAGAAATTTGCAGAACTTGATGTGAACAAGAACAAACGCTTGGAAGTGGAAGAATTGAACCCAATGCTGCATTACATTCACCCAGGAGAATTTTCCTATGCTAAATATTATGCAAGCTTTCTGATGTCGGAGGCGGATGATAACAAAGATAGAAAATTAACGCTCGAGGAGATGCTCGATCATGACTATGTTTTCTATAGCACAGTATATGATGACGGCATCAACGAAGATTTTGATGCGGATTTTCACGACGAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

395

Amino Acids

45.83

Weight (kDa)

5.43

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
AatII GACGTC 1 cut(s) 121
AccB1I GGYRCC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 18
AccII CGCG 3 cut(s) 23, 131, 222
AciI CCGC 6 cut(s) 36, 131, 805, 900, 1058, 1163
AclWI GGATC 1 cut(s) 819
AcsI RAATTY 7 cut(s) 532, 651, 835, 887, 929, 1013, 1178
AcyI GRCGYC 2 cut(s) 118, 359
AfaI GTAC 3 cut(s) 179, 686, 912
AfiI CCNNNNNNNGG 3 cut(s) 104, 368, 423
AgsI TTSAA 6 cut(s) 313, 430, 591, 887, 893, 982
AjnI CCWGG 2 cut(s) 354, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AluBI AGCT 2 cut(s) 391, 1041
AluI AGCT 2 cut(s) 391, 1041
Alw21I GWGCWC 1 cut(s) 393
AlwI GGATC 1 cut(s) 819
Ama87I CYCGRG 2 cut(s) 392, 1088
AoxI GGCC 2 cut(s) 79, 106
ApeKI GCWGC 2 cut(s) 161, 991
ApoI RAATTY 7 cut(s) 532, 651, 835, 887, 929, 1013, 1178
AseI ATTAAT 1 cut(s) 729
Asp700I GAANNNNTTC 2 cut(s) 484, 651
AspLEI GCGC 2 cut(s) 25, 361
AspS9I GGNCC 2 cut(s) 80, 523
AsuHPI GGTGA 2 cut(s) 485, 995
AvaI CYCGRG 2 cut(s) 392, 1088
AvaII GGWCC 1 cut(s) 523
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 393
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
BauI CACGAG 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 173, 978
BccI CCATC 5 cut(s) 163, 464, 470, 802, 818
BceAI ACGGC 1 cut(s) 1156
BcgI CGANNNNNNTGC 2 cut(s) 769, 803
BciT130I CCWGG 2 cut(s) 356, 1008
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 1120
BfoI RGCGCY 1 cut(s) 362
BisI GCNGC 2 cut(s) 162, 992
BlsI GCNGC 2 cut(s) 163, 993
Bme1390I CCNGG 2 cut(s) 356, 1008
Bme18I GGWCC 1 cut(s) 523
BmeT110I CYCGRG 2 cut(s) 392, 1088
BmgT120I GGNCC 2 cut(s) 80, 523
BmiI GGNNCC 2 cut(s) 82, 360
BmrFI CCNGG 2 cut(s) 356, 1008
BmsI GCATC 4 cut(s) 750, 1086, 1150, 1152
BpiI GAAGAC 1 cut(s) 639
BsaHI GRCGYC 2 cut(s) 118, 359
BsaJI CCNNGG 5 cut(s) 76, 103, 183, 417, 1006
BsaWI WCCGGW 1 cut(s) 919
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 368, 423
BseBI CCWGG 2 cut(s) 356, 1008
BseDI CCNNGG 5 cut(s) 76, 103, 183, 417, 1006
BseGI GGATG 2 cut(s) 813, 1066
BseLI CCNNNNNNNGG 3 cut(s) 104, 368, 423
BseRI GAGGAG 2 cut(s) 41, 1106
BseXI GCAGC 2 cut(s) 173, 978
Bsh1236I CGCG 3 cut(s) 23, 131, 222
BshFI GGCC 2 cut(s) 81, 108
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 2 cut(s) 392, 1088
BsiSI CCGG 1 cut(s) 920
BslI CCNNNNNNNGG 3 cut(s) 104, 368, 423
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 2 cut(s) 81, 108
BsoBI CYCGRG 2 cut(s) 392, 1088
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 2 cut(s) 811, 1102
BspACI CCGC 6 cut(s) 36, 131, 805, 900, 1058, 1163
BspANI GGCC 2 cut(s) 81, 108
BspFNI CGCG 3 cut(s) 23, 131, 222
BspHI TCATGA 2 cut(s) 405, 1105
BspLI GGNNCC 2 cut(s) 82, 360
BspPI GGATC 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 358
BssECI CCNNGG 5 cut(s) 76, 103, 183, 417, 1006
BssMI GATC 2 cut(s) 811, 1102
BssNI GRCGYC 2 cut(s) 118, 359
BssSI CACGAG 1 cut(s) 842
BssT1I CCWWGG 2 cut(s) 103, 183
Bst2BI CACGAG 1 cut(s) 842
Bst2UI CCWGG 2 cut(s) 356, 1008
Bst4CI ACNGT 5 cut(s) 77, 418, 556, 638, 1129
Bst6I CTCTTC 1 cut(s) 918
BstACI GRCGYC 2 cut(s) 118, 359
BstC8I GCNNGC 1 cut(s) 1039
BstDSI CCRYGG 2 cut(s) 76, 417
BstF5I GGATG 2 cut(s) 813, 1066
BstFNI CGCG 3 cut(s) 23, 131, 222
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 2 cut(s) 25, 361
BstKTI GATC 2 cut(s) 814, 1105
BstMBI GATC 2 cut(s) 811, 1102
BstMWI GCNNNNNNNGC 2 cut(s) 29, 137
BstNI CCWGG 2 cut(s) 356, 1008
BstSCI CCNGG 2 cut(s) 354, 1006
BstSFI CTRYAG 1 cut(s) 1120
BstUI CGCG 3 cut(s) 23, 131, 222
BstV1I GCAGC 2 cut(s) 173, 978
BstV2I GAAGAC 1 cut(s) 639
BsuRI GGCC 2 cut(s) 81, 108
BtgI CCRYGG 2 cut(s) 76, 417
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 813, 1066
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 1039
CciI TCATGA 2 cut(s) 405, 1105
CfoI GCGC 2 cut(s) 25, 361
Cfr13I GGNCC 2 cut(s) 80, 523
CseI GACGC 1 cut(s) 448
Csp6I GTAC 3 cut(s) 178, 685, 911
CspCI CAANNNNNGTGG 4 cut(s) 58, 93, 172, 207
CviAII CATG 6 cut(s) 58, 253, 284, 406, 493, 1106
CviJI RGCY 8 cut(s) 81, 108, 126, 164, 328, 391, 664, 1041
CviKI_1 RGCY 8 cut(s) 81, 108, 126, 164, 328, 391, 664, 1041
CviQI GTAC 3 cut(s) 178, 685, 911
DinI GGCGCC 1 cut(s) 360
DpnI GATC 2 cut(s) 813, 1104
DpnII GATC 2 cut(s) 811, 1102
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
EciI GGCGGA 2 cut(s) 820, 1073
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 2 cut(s) 103, 183
Eco24I GRGCYC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 523
Eco53kI GAGCTC 1 cut(s) 391
Eco88I CYCGRG 2 cut(s) 392, 1088
EcoICRI GAGCTC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 523
EcoRI GAATTC 1 cut(s) 1178
EcoRII CCWGG 2 cut(s) 354, 1006
EcoT14I CCWWGG 2 cut(s) 103, 183
EcoT22I ATGCAT 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 393
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 103, 183
FaeI CATG 6 cut(s) 61, 256, 287, 409, 496, 1109
FatI CATG 6 cut(s) 57, 252, 283, 405, 492, 1105
FauI CCCGC 2 cut(s) 124, 893
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 162, 992
FokI GGATG 2 cut(s) 820, 1073
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 2 cut(s) 162, 992
FspBI CTAG 1 cut(s) 527
GlaI GCGC 2 cut(s) 24, 360
GluI GCNGC 2 cut(s) 162, 992
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 2 cut(s) 81, 108
HapII CCGG 1 cut(s) 920
HgaI GACGC 1 cut(s) 448
HhaI GCGC 2 cut(s) 25, 361
Hin1I GRCGYC 2 cut(s) 118, 359
Hin1II CATG 6 cut(s) 61, 256, 287, 409, 496, 1109
Hin6I GCGC 2 cut(s) 23, 359
HinP1I GCGC 2 cut(s) 23, 359
HindIII AAGCTT 1 cut(s) 1039
HinfI GANTC 2 cut(s) 198, 455
HpaII CCGG 1 cut(s) 920
HphI GGTGA 2 cut(s) 485, 995
Hpy166II GTNNAC 2 cut(s) 19, 949
Hpy188I TCNGA 6 cut(s) 203, 235, 750, 1047, 1054, 1184
Hpy188III TCNNGA 4 cut(s) 406, 815, 1106, 1172
Hpy8I GTNNAC 2 cut(s) 19, 949
Hpy99I CGWCG 2 cut(s) 120, 1178
HpyAV CCTTC 1 cut(s) 548
HpyCH4III ACNGT 5 cut(s) 77, 418, 556, 638, 1129
HpyCH4IV ACGT 3 cut(s) 118, 320, 882
HpyCH4V TGCA 7 cut(s) 283, 615, 625, 763, 935, 994, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 137
HpySE526I ACGT 3 cut(s) 118, 320, 882
Hsp92I GRCGYC 2 cut(s) 118, 359
Hsp92II CATG 6 cut(s) 61, 256, 287, 409, 496, 1109
HspAI GCGC 2 cut(s) 23, 359
KasI GGCGCC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 811, 1102
Lsp1109I GCAGC 2 cut(s) 173, 978
LweI GCATC 4 cut(s) 750, 1086, 1150, 1152
MaeI CTAG 1 cut(s) 527
MaeII ACGT 3 cut(s) 118, 320, 882
MaeIII GTNAC 2 cut(s) 134, 638
MalI GATC 2 cut(s) 813, 1104
MboI GATC 2 cut(s) 811, 1102
MboII GAAGA 6 cut(s) 644, 659, 694, 935, 986, 1163
MhlI GDGCHC 1 cut(s) 393
Mly113I GGCGCC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 213, 1032
Mph1103I ATGCAT 1 cut(s) 285
MroXI GAANNNNTTC 2 cut(s) 484, 651
MseI TTAA 5 cut(s) 378, 729, 737, 776, 1082
MslI CAYNNNNRTG 2 cut(s) 729, 1110
MspI CCGG 1 cut(s) 920
MspR9I CCNGG 2 cut(s) 356, 1008
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 2 cut(s) 356, 1008
MvnI CGCG 3 cut(s) 23, 131, 222
MwoI GCNNNNNNNGC 2 cut(s) 29, 137
NarI GGCGCC 1 cut(s) 359
NdeII GATC 2 cut(s) 811, 1102
NlaIII CATG 6 cut(s) 61, 256, 287, 409, 496, 1109
NlaIV GGNNCC 2 cut(s) 82, 360
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 134
NsiI ATGCAT 1 cut(s) 285
PaeR7I CTCGAG 2 cut(s) 392, 1088
PagI TCATGA 2 cut(s) 405, 1105
PcsI WCGNNNNNNNCGW 1 cut(s) 1146
PctI GAATGC 1 cut(s) 265
PdmI GAANNNNTTC 2 cut(s) 484, 651
PfeI GAWTC 2 cut(s) 198, 455
PkrI GCNGC 2 cut(s) 163, 993
PluTI GGCGCC 1 cut(s) 362
PpuMI RGGWCCY 1 cut(s) 523
PshBI ATTAAT 1 cut(s) 729
PsiI TTATAA 1 cut(s) 794
Psp124BI GAGCTC 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 354, 1006
PspGI CCWGG 2 cut(s) 354, 1006
PspN4I GGNNCC 2 cut(s) 82, 360
PspPI GGNCC 2 cut(s) 80, 523
PspPPI RGGWCCY 1 cut(s) 523
PspXI VCTCGAGB 2 cut(s) 392, 1088
RsaI GTAC 3 cut(s) 179, 686, 912
RsaNI GTAC 3 cut(s) 178, 685, 911
RseI CAYNNNNRTG 2 cut(s) 729, 1110
SacI GAGCTC 1 cut(s) 393
SaqAI TTAA 5 cut(s) 378, 729, 737, 776, 1082
SatI GCNGC 2 cut(s) 162, 992
Sau3AI GATC 2 cut(s) 811, 1102
Sau96I GGNCC 2 cut(s) 80, 523
ScrFI CCNGG 2 cut(s) 356, 1008
SduI GDGCHC 1 cut(s) 393
SfaNI GCATC 4 cut(s) 750, 1086, 1150, 1152
SfcI CTRYAG 1 cut(s) 1120
SfoI GGCGCC 1 cut(s) 360
Sfr274I CTCGAG 2 cut(s) 392, 1088
SinI GGWCC 1 cut(s) 523
SlaI CTCGAG 2 cut(s) 392, 1088
SmiMI CAYNNNNRTG 2 cut(s) 729, 1110
SmlI CTYRAG 2 cut(s) 392, 1088
SmoI CTYRAG 2 cut(s) 392, 1088
SsiI CCGC 6 cut(s) 36, 131, 805, 900, 1058, 1163
SspDI GGCGCC 1 cut(s) 358
SspI AATATT 1 cut(s) 1031
SspMI CTAG 1 cut(s) 527
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 2 cut(s) 354, 1006
StyI CCWWGG 2 cut(s) 103, 183
TaaI ACNGT 5 cut(s) 77, 418, 556, 638, 1129
TaiI ACGT 3 cut(s) 121, 323, 885
TaqI TCGA 4 cut(s) 150, 393, 1089, 1101
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 2 cut(s) 198, 455
Tru1I TTAA 5 cut(s) 378, 729, 737, 776, 1082
Tru9I TTAA 5 cut(s) 378, 729, 737, 776, 1082
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 2 cut(s) 161, 991
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 6 cut(s) 272, 300, 422, 481, 530, 695
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 523
VspI ATTAAT 1 cut(s) 729
XapI RAATTY 7 cut(s) 532, 651, 835, 887, 929, 1013, 1178
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XhoI CTCGAG 2 cut(s) 392, 1088
XmiI GTMKAC 1 cut(s) 18
XmnI GAANNNNTTC 2 cut(s) 484, 651
XspI CTAG 1 cut(s) 527
ZraI GACGTC 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.