RLG00000026216

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013165
Physical Location & Seq
Forward (+)
1317235 .. 1318950
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026216

Sequence Viewer

Length: 1041 bp
ATGGCGAAGGCGGTGGTCTATCTTCTATTCGCCACCGCCCTTATTGCTCTTATAGTCCTCTCTCCCTTCAAGCCACGGTGCCACAACGTCCATGGCCTGCACCGCCGCCTTGGCTTCACCGGCCACGTCCCTATCTTCGATCCTCTTGTTTCCAAGATGGAATGGTACGTGGAGGAAAAGGGATTAGGAGACCAAAACATTACTCTAGATACGGAACACGACTTGGTTATTGCCAATGATGTTGAGGAAGCACAGGAATTTTTGAGCGAGGCGGGAACGTTGAATATAACCATGAGGCTCGTGAATATCTTCCCTTTGTTGGATGTGGCACCCGAAGATGGATTCGTTAGCTACAGTGAGCTGAAGCATTGGATTGTGGCACAAGCCGTGGAAAGAATGATTCATAGGACACAGAATGAAATGGCATCCCATGATAGGGATGGAGATCGAGCCATTTCTTTCAGGGAGTACCTGCCACAATTTTCCATTGAGGATATTGATAGAAATGATATGGAGCATGGACAAGCTGGATGGTGGAAGCAGCAATTTGATAACGCAGATGCTGATAGGAATGGTTTACTTACCTTCAATGAACTTAAAGATTTTTTGCACCCAGAAGACAGTTCAAATGACAGAATTCATAAATGGCTCTTGACAGAAAAAATGAAAAGGATGGACCATGATAATGATGGCAAGCTGAACTTCCAGGAGTTTTCGGACAATGCTTATGATATCTTCAAGAATTATGTTGAATTTGAGACAGGCGGAGTACCCACATCAGAAGATAAATTTGCAGAGCTTGATGTGAACAAAAACAAATTCATAGAAGCTGAAGAATTGAAACCCATACTGCATTATCTTTACCCAGGAGAGCTAACCTATGCTACCTATTTCACAAGTTTTCTAATTCACGAGGCTGATGATAACAAAGATGAAAAATTAACGTTGCAAGAGATGCTGCATCACGAAGATATTTTCTACAACACAGTCTATGAGAGCAGCAATGACGATGACAACCATGATGATCACGACGAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

347

Amino Acids

40.33

Weight (kDa)

4.63

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 179 - 239 2.7e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 480
AccB1I GGYRCC 2 cut(s) 78, 328
AciI CCGC 6 cut(s) 11, 36, 103, 106, 272, 765
AclI AACGTT 2 cut(s) 278, 944
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 6 cut(s) 257, 636, 752, 788, 818, 1034
AcuI CTGAAG 2 cut(s) 383, 852
AfaI GTAC 3 cut(s) 167, 470, 771
AfiI CCNNNNNNNGG 4 cut(s) 319, 338, 435, 436
AgsI TTSAA 7 cut(s) 70, 283, 589, 627, 739, 752, 841
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 2 cut(s) 705, 865
AluBI AGCT 7 cut(s) 351, 361, 527, 697, 799, 830, 874
AluI AGCT 7 cut(s) 351, 361, 527, 697, 799, 830, 874
Alw26I GTCTC 2 cut(s) 183, 752
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 2 cut(s) 94, 121
ApeKI GCWGC 3 cut(s) 541, 958, 999
ApoI RAATTY 6 cut(s) 257, 636, 752, 788, 818, 1034
Asp700I GAANNNNTTC 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 676
BanI GGYRCC 2 cut(s) 78, 328
BauI CACGAG 2 cut(s) 299, 911
BbsI GAAGAC 1 cut(s) 624
BbvI GCAGC 3 cut(s) 553, 945, 1011
BccI CCATC 6 cut(s) 151, 332, 434, 525, 667, 683
BceAI ACGGC 1 cut(s) 371
BciT130I CCWGG 2 cut(s) 707, 867
BclI TGATCA 1 cut(s) 1024
BcoDI GTCTC 2 cut(s) 183, 752
BfaI CTAG 1 cut(s) 206
BfmI CTRYAG 1 cut(s) 352
BfuAI ACCTGC 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 4 cut(s) 106, 542, 959, 1000
BlsI GCNGC 4 cut(s) 107, 543, 960, 1001
Bme1390I CCNGG 2 cut(s) 707, 867
Bme18I GGWCC 1 cut(s) 676
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 2 cut(s) 80, 330
BmrFI CCNGG 2 cut(s) 707, 867
BmsI GCATC 4 cut(s) 434, 550, 945, 970
BpiI GAAGAC 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 74, 91, 109, 387, 865
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 338, 435, 436
Bse118I RCCGGY 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 1009
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 2 cut(s) 707, 867
BseDI CCNNGG 5 cut(s) 74, 91, 109, 387, 865
BseGI GGATG 5 cut(s) 328, 425, 445, 536, 678
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 4 cut(s) 319, 338, 435, 436
BseMI GCAATG 1 cut(s) 1009
BseXI GCAGC 3 cut(s) 553, 945, 1011
BsgI GTGCAG 1 cut(s) 83
BshFI GGCC 2 cut(s) 96, 123
BshNI GGYRCC 2 cut(s) 78, 328
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 4 cut(s) 319, 338, 435, 436
BsmAI GTCTC 2 cut(s) 183, 752
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 2 cut(s) 96, 123
Bso31I GGTCTC 1 cut(s) 183
Bsp143I GATC 3 cut(s) 139, 445, 1024
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 6 cut(s) 11, 36, 103, 106, 272, 765
BspANI GGCC 2 cut(s) 96, 123
BspLI GGNNCC 2 cut(s) 80, 330
BspMI ACCTGC 1 cut(s) 480
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 2 cut(s) 78, 328
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 1009
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 5 cut(s) 74, 91, 109, 387, 865
BssMI GATC 3 cut(s) 139, 445, 1024
BssSI CACGAG 2 cut(s) 299, 911
BssT1I CCWWGG 2 cut(s) 91, 109
Bst2BI CACGAG 2 cut(s) 299, 911
Bst2UI CCWGG 2 cut(s) 707, 867
Bst4CI ACNGT 4 cut(s) 78, 356, 623, 988
BstAPI GCANNNNNTGC 1 cut(s) 955
BstBAI YACGTR 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 98, 695
BstDSI CCRYGG 3 cut(s) 74, 91, 387
BstF5I GGATG 5 cut(s) 328, 425, 445, 536, 678
BstKTI GATC 3 cut(s) 142, 448, 1027
BstMAI GTCTC 2 cut(s) 183, 752
BstMBI GATC 3 cut(s) 139, 445, 1024
BstMWI GCNNNNNNNGC 5 cut(s) 44, 102, 111, 120, 955
BstNI CCWGG 2 cut(s) 707, 867
BstSCI CCNGG 2 cut(s) 705, 865
BstSFI CTRYAG 1 cut(s) 352
BstV1I GCAGC 3 cut(s) 553, 945, 1011
BstV2I GAAGAC 1 cut(s) 624
BsuRI GGCC 2 cut(s) 96, 123
BtgI CCRYGG 3 cut(s) 74, 91, 387
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 5 cut(s) 328, 425, 445, 536, 678
BtsIMutI CAGTG 1 cut(s) 361
BveI ACCTGC 1 cut(s) 480
Cac8I GCNNGC 2 cut(s) 98, 695
CaiI CAGNNNCTG 1 cut(s) 563
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 676
Csp6I GTAC 3 cut(s) 166, 469, 770
CviAII CATG 6 cut(s) 92, 292, 431, 518, 680, 1019
CviQI GTAC 3 cut(s) 166, 469, 770
DpnI GATC 3 cut(s) 141, 447, 1026
DpnII GATC 3 cut(s) 139, 445, 1024
EaeI YGGCCR 1 cut(s) 121
EciI GGCGGA 1 cut(s) 780
Eco130I CCWWGG 2 cut(s) 91, 109
Eco31I GGTCTC 1 cut(s) 183
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 1 cut(s) 676
Eco57I CTGAAG 2 cut(s) 383, 852
EcoRI GAATTC 2 cut(s) 636, 1034
EcoRII CCWGG 2 cut(s) 705, 865
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 2 cut(s) 91, 109
ErhI CCWWGG 2 cut(s) 91, 109
FaeI CATG 6 cut(s) 95, 295, 434, 521, 683, 1022
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FaqI GGGAC 1 cut(s) 113
FatI CATG 6 cut(s) 91, 291, 430, 517, 679, 1018
FauI CCCGC 1 cut(s) 265
FbaI TGATCA 1 cut(s) 1024
Fnu4HI GCNGC 4 cut(s) 106, 542, 959, 1000
FokI GGATG 5 cut(s) 335, 412, 452, 543, 685
Fsp4HI GCNGC 4 cut(s) 106, 542, 959, 1000
FspBI CTAG 1 cut(s) 206
GluI GCNGC 4 cut(s) 106, 542, 959, 1000
HaeIII GGCC 2 cut(s) 96, 123
HapII CCGG 1 cut(s) 120
Hin1II CATG 6 cut(s) 95, 295, 434, 521, 683, 1022
HinfI GANTC 2 cut(s) 342, 400
HpaII CCGG 1 cut(s) 120
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 578, 808
Hpy188I TCNGA 2 cut(s) 718, 781
Hpy188III TCNNGA 7 cut(s) 206, 301, 652, 739, 911, 965, 1028
Hpy8I GTNNAC 2 cut(s) 578, 808
Hpy99I CGWCG 1 cut(s) 1034
HpyAV CCTTC 2 cut(s) 76, 595
HpyCH4III ACNGT 4 cut(s) 78, 356, 623, 988
HpyCH4IV ACGT 5 cut(s) 87, 126, 168, 278, 944
HpyCH4V TGCA 6 cut(s) 100, 610, 794, 853, 949, 961
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 102, 111, 120, 955
HpySE526I ACGT 5 cut(s) 87, 126, 168, 278, 944
Hsp92II CATG 6 cut(s) 95, 295, 434, 521, 683, 1022
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 3 cut(s) 139, 445, 1024
LmnI GCTCC 1 cut(s) 514
Lsp1109I GCAGC 3 cut(s) 553, 945, 1011
LweI GCATC 4 cut(s) 434, 550, 945, 970
MaeI CTAG 1 cut(s) 206
MaeII ACGT 5 cut(s) 87, 126, 168, 278, 944
MalI GATC 3 cut(s) 141, 447, 1026
MboI GATC 3 cut(s) 139, 445, 1024
MboII GAAGA 9 cut(s) 14, 127, 301, 347, 629, 727, 794, 845, 980
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 8 cut(s) 68, 153, 166, 238, 262, 288, 484, 907
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 2 cut(s) 597, 941
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 707, 867
MvaI CCWGG 2 cut(s) 707, 867
MwoI GCNNNNNNNGC 5 cut(s) 44, 102, 111, 120, 955
NcoI CCATGG 1 cut(s) 91
NdeII GATC 3 cut(s) 139, 445, 1024
NlaIII CATG 6 cut(s) 95, 295, 434, 521, 683, 1022
NlaIV GGNNCC 2 cut(s) 80, 330
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 2 cut(s) 342, 400
PfoI TCCNGGA 1 cut(s) 705
PkrI GCNGC 4 cut(s) 107, 543, 960, 1001
Ppu21I YACGTR 1 cut(s) 169
Psp1406I AACGTT 2 cut(s) 278, 944
Psp6I CCWGG 2 cut(s) 705, 865
PspGI CCWGG 2 cut(s) 705, 865
PspN4I GGNNCC 2 cut(s) 80, 330
PspPI GGNCC 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 563
RsaI GTAC 3 cut(s) 167, 470, 771
RsaNI GTAC 3 cut(s) 166, 469, 770
RseI CAYNNNNRTG 1 cut(s) 684
SaqAI TTAA 2 cut(s) 597, 941
SatI GCNGC 4 cut(s) 106, 542, 959, 1000
Sau3AI GATC 3 cut(s) 139, 445, 1024
Sau96I GGNCC 1 cut(s) 676
ScrFI CCNGG 2 cut(s) 707, 867
SfaNI GCATC 4 cut(s) 434, 550, 945, 970
SfcI CTRYAG 1 cut(s) 352
SinI GGWCC 1 cut(s) 676
SmiMI CAYNNNNRTG 1 cut(s) 684
SsiI CCGC 6 cut(s) 11, 36, 103, 106, 272, 765
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 705, 865
StyI CCWWGG 2 cut(s) 91, 109
TaaI ACNGT 4 cut(s) 78, 356, 623, 988
TaiI ACGT 5 cut(s) 90, 129, 171, 281, 947
TaqI TCGA 2 cut(s) 138, 448
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 2 cut(s) 342, 400
Tru1I TTAA 2 cut(s) 597, 941
Tru9I TTAA 2 cut(s) 597, 941
TscAI CASTG 1 cut(s) 361
TseI GCWGC 3 cut(s) 541, 958, 999
TspDTI ATGAA 7 cut(s) 392, 432, 606, 629, 680, 811, 948
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 676
XapI RAATTY 6 cut(s) 257, 636, 752, 788, 818, 1034
XbaI TCTAGA 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 3 cut(s) 89, 437, 686
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.