Rorug01G0430300

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002290
Physical Location & Seq
Reverse (-)
52878825 .. 52885854
7030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0430300.1

Sequence Viewer

Length: 2841 bp
ATGGCAATCAAGGCCTCGTTCAGCAATGTAGCCAATGTTTTACTGGACTGGAATGATGCTCATGACGAAGATTTCTGTTCTTGGCGTGGAGTATTCTGTGACAATGTCAGCCTCTCTGTGGCCTCCTTGAATTTGTCCAGTTTGAATCTGGGCGGGGAGATTTCGCCAGCCCTTGGAGATTTGGGAAACTTGGAATCCATAGACTTGCAGGGGAATAAGCTAACTGGTCAAATCCCGGATGAGATTGGCAACTGTGCTTCTCTTATATATCTGGATTTGTCTGACAATAATCTGTCTGGAGACATACCCTTCTCGGTTTCAAAGCTTAAGAAGCTTGAGGTTTTTAATTTGAAGAACAATCAGTTGACTGGTCCTATCCCTACAACTCTTACCCAAATTCCAAACCTTAAAACTCTTGATCTTGCTCGGAACCAGCTCACTGGTGAGATACCAAGGCTAATCTATTGGAATGAAGTTTTGCAGTACCTTGGATTGCGAGGCAACTCTTTGACTGGAGCTCTGTCCCCTGATATGTGTCAATTAACTGGTCTGTGGTACTTTGATGTTAGAGGCAATAACCTGACTGGCCCGATCCCCGACAACATTGGCAACTGTACAAGCTTTGAGATCCTGGACATATCATACAATCAGATCACTGGTGGGATTCCATATAACATTGGATTTTTACAAGTGGCTACCCTGTCGCTACAAGGGAATAGACTGACTGGAAAGATTCCTGAGGTTATTGGTTTAATGCAGGCTCTTGCTGTTTTGGATTTAAGTGAGAATGAACTTGTTGGGCCAATTCCACCGATACTAGGCAATTTATCATACACTGGGAAACTATACCTGCATGGTAACAAGTTGACTGGGACAATTCCTCCTGAACTTGGCAATATGTCAAAGCTTAGCTATTTGCAATTAAATGACAACAATCTGGTTGGTAGAATTCCTGGTGAGCTTGGGAAGCTGAACCAGTTGTTTGAATTGAATCTTGCCAACAATGATCTGGAAGGTCCCATCCCACATGACATCGGTTCCTGCACTGCCTTGAACCAATTCAACGTTCATGGAAACCGCTTGACAGGATCCATTCCTTTAGCATTGAAAAATCTGCAGAGCTTGACCTATCTAAATTTGTCAGCAAATGCCTTCAGTGGTAGAATTAATCTTGAGTTGGGACATATCATCAATCTTGACACTTTGGATTTGTCCAGCAACAATTTCTCTGGCCCTGTTCCTGCTTCTGTTGGCAATCTTGAGCACCTCCTGACTTTGAATTTGAGTGGGAATCATCTTGATGGTCCTTTGCCTGCAGAATTTGGGGACCTCAGGAGTGTTCAAATCATTGATATGTCATTCAACAATCTCTCTGGGAGCATTCCCTCAGAATTGGGTCAGTTGCAAAACCTTGCGGCATTGTTGCTTAATAACAACAACTTGCGTGGAAAGATACCTGATCAGCTAACCAACTGTTTCAGCCTTGAGACCTTGAATTTTTCATACAACAATCTGTCGGGCATTGTACCTCCCATGAGGAACTTTTCACGTTTTTCACCAAACAGCTTCATTGCAAACCCGCTGTTGTGCAGTGACTGGTTGGGATCGATATGCAGACCCGTTGCCCCCAAGTCCAGGGCTGTATTCTCCCGAGCAGCAGTTGTCTGTATGGCGTTAGGCTTCATCACATTATTATCCATGGTAATAGTTGCGATTTACAAGTCCAACCAACCAAAGCAGTTATTGATGGGATCTACCAAGAACGGACAAGGTCCTGCTAAACTTGTAATCCTTCACATGGATATGGCTATACACAGCTTTGATGATATAATGAGAATCACTGAGAATCTAAATGAGAAGTATATCATAGGGTATGGTGCTTCTAGCACAGTGTACAAGTGTGTACTGAAAAATTCCCGACCAATGGCAATCAAGCGACTCTACAACCGGTATCCACACAACTGGAGGGAGTTTGAGACGGAACTTGAAACCATTGGCAGTATCAAGCATAGAAATTTAGTCAGTTTGCATGGGTATTCACTGTCTCCCTTGGGCAATCTCCTCTTCTATGACTACATGGAGAACGGGTCTCTGTGGGATATGCTTCATGGACCATCGAAAAAGGTTAAACTTGATTGGGAAACACGTTTGAAAATAGCAGTTGGAGCAGCTCAAGGACTTGCTTATCTTCACCATGACTGCAACCCCCGCATTATTCACAGGGATGTGAAATCCTCTAATATCCTGCTGGATGAGAATTTTGAAGCTCATCTCTCTGATTTTGGAATTGCAAAATCCATCCCCAGTGCGGATACCCATGCATCGACTTATGTTCTGGGAACAATTGGTTACATCGATCCAGAATATGCTAGGACTTCTCGGCTCAATGAAAAATCTGATGTATATAGCTTTGGCATTGTGCTTCTTGAGCTACTGACTGGGAAGAAGGCTGTGGACAAGGAGTCTAACTTGCATCATCTGATATTGTCCAAGGCAGATAACAATACAGTAATGGAGACTGTTGACCCTGAAGTTTCTGTCACCTGCATGGATATAACTCATGTCAAGAAAACTTTTCAACTCGCCCTGCTGTGCACCAAGCGAAACCCCTCTGAGAGGCCAACAATGCATGAAGTTGCAAGGGTTTTGGTCTCCCTCCTACCTTTACATCACTCAAAGAAGCCTCTTTCAAAGGGTATCGACTATGATAAGTTTGCGGTTAACAAGGAACAACAACAACCAAAAGTGCAACAGCAGAAGCCTGTTCGGCAAGAAAGTCATTCATCTGATGCTCAATGGTTTCTTCGGTTTGGTGAGGTTATATCCAAGAACACACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

946

Amino Acids

104.25

Weight (kDa)

6.22

Isoelectric Point (pI)

27.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 1 - 34 5.1e-07 Leucine rich repeat N-terminal domain
LRR_4 PF12799 87 - 123 6.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 88 - 146 3.7e-08 Leucine rich repeat
LRR_8 PF13855 281 - 337 2.5e-06 Leucine rich repeat
LRR_14 PF23598 296 - 406 3.2e-08 Leucine-rich repeat region
LRR_8 PF13855 398 - 457 2.9e-08 Leucine rich repeat
LRR_14 PF23598 416 - 500 4.1e-08 Leucine-rich repeat region
Pkinase PF00069 619 - 880 6.7e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 622 - 882 1.3e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2552
Acc36I ACCTGC 2 cut(s) 858, 2552
AccB7I CCANNNNNTGG 1 cut(s) 173
AciI CCGC 7 cut(s) 153, 1078, 1415, 1580, 2209, 2309, 2717
AclI AACGTT 1 cut(s) 1065
AclWI GGATC 7 cut(s) 586, 622, 1083, 1096, 1612, 1759, 2351
AcuI CTGAAG 2 cut(s) 1138, 2550
AfaI GTAC 6 cut(s) 485, 557, 616, 1527, 1895, 1905
AfiI CCNNNNNNNGG 9 cut(s) 118, 173, 818, 890, 1635, 1798, 1924, 2308, 2616
AflII CTTAAG 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 2144
AgeI ACCGGT 1 cut(s) 1947
AhdI GACNNNNNGTC 1 cut(s) 2461
AjnI CCWGG 3 cut(s) 630, 952, 1634
AjuI GAANNNNNNNTTGG 2 cut(s) 1050, 1082
Alw21I GWGCWC 3 cut(s) 520, 1266, 2597
Alw26I GTCTC 7 cut(s) 294, 1481, 1970, 2049, 2094, 2510, 2656
Alw44I GTGCAC 1 cut(s) 2593
AlwI GGATC 7 cut(s) 586, 622, 1083, 1096, 1612, 1759, 2351
AlwNI CAGNNNCTG 1 cut(s) 1596
Ama87I CYCGRG 1 cut(s) 1650
AoxI GGCC 6 cut(s) 12, 120, 586, 800, 1231, 2618
ApaLI GTGCAC 1 cut(s) 2593
ApeKI GCWGC 2 cut(s) 1655, 2168
ArsI GACNNNNNNTTYG 2 cut(s) 1264, 1296
AseI ATTAAT 1 cut(s) 1167
AsiGI ACCGGT 1 cut(s) 1947
Asp700I GAANNNNTTC 1 cut(s) 1058
AspS9I GGNCC 9 cut(s) 371, 587, 800, 1016, 1232, 1304, 1327, 1772, 2111
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 6 cut(s) 455, 968, 1548, 2183, 2533, 2825
AvaI CYCGRG 1 cut(s) 1650
AvaII GGWCC 6 cut(s) 371, 1016, 1304, 1327, 1772, 2111
AxyI CCTNAGG 2 cut(s) 738, 1331
BaeGI GKGCMC 1 cut(s) 2597
BamHI GGATCC 1 cut(s) 1088
BanII GRGCYC 1 cut(s) 520
Bbv12I GWGCWC 3 cut(s) 520, 1266, 2597
BbvI GCAGC 2 cut(s) 1667, 2180
BccI CCATC 5 cut(s) 1028, 1295, 1741, 2122, 2306
BciT130I CCWGG 3 cut(s) 632, 954, 1636
BciVI GTATCC 2 cut(s) 1962, 2305
BclI TGATCA 1 cut(s) 1459
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 7 cut(s) 294, 1481, 1970, 2049, 2094, 2510, 2656
BfaI CTAG 3 cut(s) 818, 1884, 2370
BfmI CTRYAG 2 cut(s) 1115, 1314
BfrI CTTAAG 1 cut(s) 326
BfuAI ACCTGC 2 cut(s) 858, 2552
BfuI GTATCC 2 cut(s) 1962, 2305
BglI GCCNNNNNGGC 1 cut(s) 2767
BisI GCNGC 3 cut(s) 1416, 1656, 2169
BlpI GCTNAGC 1 cut(s) 908
BlsI GCNGC 3 cut(s) 1417, 1657, 2170
Bme1390I CCNGG 4 cut(s) 236, 632, 954, 1636
Bme18I GGWCC 6 cut(s) 371, 1016, 1304, 1327, 1772, 2111
BmeRI GACNNNNNGTC 1 cut(s) 2461
BmeT110I CYCGRG 1 cut(s) 1650
BmgT120I GGNCC 9 cut(s) 371, 587, 800, 1016, 1232, 1304, 1327, 1772, 2111
BmiI GGNNCC 5 cut(s) 431, 1018, 1039, 1090, 1328
BmrFI CCNGG 4 cut(s) 236, 632, 954, 1636
BmrI ACTGGG 4 cut(s) 846, 879, 2298, 2448
BmsI GCATC 4 cut(s) 46, 2330, 2482, 2779
BmuI ACTGGG 4 cut(s) 846, 879, 2298, 2448
BpmI CTGGAG 3 cut(s) 318, 534, 1984
Bpu1102I GCTNAGC 1 cut(s) 908
BpuEI CTTGAG 6 cut(s) 356, 1193, 1280, 1505, 2157, 2447
BpuMI CCSGG 1 cut(s) 236
Bsa29I ATCGAT 2 cut(s) 1607, 2355
BsaI GGTCTC 3 cut(s) 1481, 2094, 2656
BsaJI CCNNGG 7 cut(s) 172, 452, 487, 1635, 1698, 2049, 2490
BsaWI WCCGGW 1 cut(s) 1947
BsaXI ACNNNNNCTCC 4 cut(s) 865, 895, 2072, 2102
Bsc4I CCNNNNNNNGG 9 cut(s) 118, 173, 818, 890, 1635, 1798, 1924, 2308, 2616
Bse118I RCCGGY 1 cut(s) 1947
Bse21I CCTNAGG 2 cut(s) 738, 1331
Bse3DI GCAATG 2 cut(s) 31, 1569
BseBI CCWGG 3 cut(s) 632, 954, 1636
BseCI ATCGAT 2 cut(s) 1607, 2355
BseDI CCNNGG 7 cut(s) 172, 452, 487, 1635, 1698, 2049, 2490
BseGI GGATG 5 cut(s) 244, 1020, 2230, 2257, 2298
BseLI CCNNNNNNNGG 9 cut(s) 118, 173, 818, 890, 1635, 1798, 1924, 2308, 2616
BseMI GCAATG 2 cut(s) 31, 1569
BseMII CTCAG 5 cut(s) 729, 1345, 1401, 1833, 2604
BseRI GAGGAG 1 cut(s) 2051
BseSI GKGCMC 1 cut(s) 2597
BseXI GCAGC 2 cut(s) 1667, 2180
BsgI GTGCAG 2 cut(s) 1027, 1609
BshFI GGCC 6 cut(s) 14, 122, 588, 802, 1233, 2620
BshTI ACCGGT 1 cut(s) 1947
BshVI ATCGAT 2 cut(s) 1607, 2355
BsiHKAI GWGCWC 3 cut(s) 520, 1266, 2597
BsiHKCI CYCGRG 1 cut(s) 1650
BsiSI CCGG 2 cut(s) 236, 1948
BslFI GGGAC 5 cut(s) 508, 886, 1002, 1194, 1340
BslI CCNNNNNNNGG 9 cut(s) 118, 173, 818, 890, 1635, 1798, 1924, 2308, 2616
BsmAI GTCTC 7 cut(s) 294, 1481, 1970, 2049, 2094, 2510, 2656
BsmBI CGTCTC 1 cut(s) 1970
BsmFI GGGAC 5 cut(s) 508, 886, 1002, 1194, 1340
BsmI GAATGC 1 cut(s) 1380
BsnI GGCC 6 cut(s) 14, 122, 588, 802, 1233, 2620
Bso31I GGTCTC 3 cut(s) 1481, 2094, 2656
BsoBI CYCGRG 1 cut(s) 1650
Bsp1286I GDGCHC 3 cut(s) 520, 1266, 2597
Bsp1407I TGTACA 2 cut(s) 614, 1893
Bsp1720I GCTNAGC 1 cut(s) 908
Bsp19I CCATGG 1 cut(s) 1698
BspACI CCGC 7 cut(s) 153, 1078, 1415, 1580, 2209, 2309, 2717
BspANI GGCC 6 cut(s) 14, 122, 588, 802, 1233, 2620
BspCNI CTCAG 5 cut(s) 730, 1344, 1400, 1834, 2605
BspDI ATCGAT 2 cut(s) 1607, 2355
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 5 cut(s) 431, 1018, 1039, 1090, 1328
BspMAI CTGCAG 2 cut(s) 1119, 1318
BspMI ACCTGC 2 cut(s) 858, 2552
BspPI GGATC 7 cut(s) 586, 622, 1083, 1096, 1612, 1759, 2351
BspTI CTTAAG 1 cut(s) 326
BspTNI GGTCTC 3 cut(s) 1481, 2094, 2656
BsrDI GCAATG 2 cut(s) 31, 1569
BsrFI RCCGGY 1 cut(s) 1947
BsrGI TGTACA 2 cut(s) 614, 1893
BssAI RCCGGY 1 cut(s) 1947
BssECI CCNNGG 7 cut(s) 172, 452, 487, 1635, 1698, 2049, 2490
BssT1I CCWWGG 6 cut(s) 172, 452, 487, 1698, 2049, 2490
Bst2UI CCWGG 3 cut(s) 632, 954, 1636
Bst4CI ACNGT 7 cut(s) 254, 614, 1475, 1891, 2043, 2509, 2521
Bst6I CTCTTC 1 cut(s) 2069
BstAFI CTTAAG 1 cut(s) 326
BstAUI TGTACA 2 cut(s) 614, 1893
BstC8I GCNNGC 3 cut(s) 168, 759, 1314
BstDEI CTNAG 6 cut(s) 738, 908, 1331, 1387, 1842, 2613
BstDSI CCRYGG 1 cut(s) 1698
BstENI CCTNNNNNAGG 1 cut(s) 2614
BstF5I GGATG 5 cut(s) 244, 1020, 2230, 2257, 2298
BstMAI GTCTC 7 cut(s) 294, 1481, 1970, 2049, 2094, 2510, 2656
BstMWI GCNNNNNNNGC 8 cut(s) 11, 331, 967, 2165, 2208, 2428, 2626, 2767
BstNI CCWGG 3 cut(s) 632, 954, 1636
BstSCI CCNGG 4 cut(s) 234, 630, 952, 1634
BstSFI CTRYAG 2 cut(s) 1115, 1314
BstSLI GKGCMC 1 cut(s) 2597
BstV1I GCAGC 2 cut(s) 1667, 2180
BstX2I RGATCY 3 cut(s) 627, 1088, 1751
BstXI CCANNNNNNTGG 2 cut(s) 440, 1962
BstYI RGATCY 3 cut(s) 627, 1088, 1751
Bsu15I ATCGAT 2 cut(s) 1607, 2355
Bsu36I CCTNAGG 2 cut(s) 738, 1331
BsuI GTATCC 2 cut(s) 1962, 2305
BsuRI GGCC 6 cut(s) 14, 122, 588, 802, 1233, 2620
BsuTUI ATCGAT 2 cut(s) 1607, 2355
BtgI CCRYGG 1 cut(s) 1698
BtsCI GGATG 5 cut(s) 244, 1020, 2230, 2257, 2298
BtsI GCAGTG 2 cut(s) 1044, 1597
BveI ACCTGC 2 cut(s) 858, 2552
Cac8I GCNNGC 3 cut(s) 168, 759, 1314
CaiI CAGNNNCTG 1 cut(s) 1596
CciI TCATGA 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 1947
Cfr13I GGNCC 9 cut(s) 371, 587, 800, 1016, 1232, 1304, 1327, 1772, 2111
ClaI ATCGAT 2 cut(s) 1607, 2355
Csp6I GTAC 6 cut(s) 484, 556, 615, 1526, 1894, 1904
CspAI ACCGGT 1 cut(s) 1947
CspCI CAANNNNNGTGG 2 cut(s) 1426, 1461
CviQI GTAC 6 cut(s) 484, 556, 615, 1526, 1894, 1904
DdeI CTNAG 6 cut(s) 738, 908, 1331, 1387, 1842, 2613
DriI GACNNNNNGTC 1 cut(s) 2461
Eam1104I CTCTTC 1 cut(s) 2069
Eam1105I GACNNNNNGTC 1 cut(s) 2461
EarI CTCTTC 1 cut(s) 2069
Ecl136II GAGCTC 1 cut(s) 518
Eco130I CCWWGG 6 cut(s) 172, 452, 487, 1698, 2049, 2490
Eco147I AGGCCT 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 520
Eco31I GGTCTC 3 cut(s) 1481, 2094, 2656
Eco47I GGWCC 6 cut(s) 371, 1016, 1304, 1327, 1772, 2111
Eco53kI GAGCTC 1 cut(s) 518
Eco57I CTGAAG 2 cut(s) 1138, 2550
Eco81I CCTNAGG 2 cut(s) 738, 1331
Eco88I CYCGRG 1 cut(s) 1650
EcoICRI GAGCTC 1 cut(s) 518
EcoNI CCTNNNNNAGG 1 cut(s) 2614
EcoO109I RGGNCCY 3 cut(s) 1016, 1327, 1772
EcoRI GAATTC 1 cut(s) 948
EcoRII CCWGG 3 cut(s) 630, 952, 1634
EcoT14I CCWWGG 6 cut(s) 172, 452, 487, 1698, 2049, 2490
EcoT22I ATGCAT 2 cut(s) 2323, 2631
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 6 cut(s) 172, 452, 487, 1698, 2049, 2490
Esp3I CGTCTC 1 cut(s) 1970
FaqI GGGAC 5 cut(s) 508, 886, 1002, 1194, 1340
FauI CCCGC 3 cut(s) 146, 1587, 2216
FbaI TGATCA 1 cut(s) 1459
Fnu4HI GCNGC 3 cut(s) 1416, 1656, 2169
FokI GGATG 5 cut(s) 251, 1007, 2237, 2264, 2285
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 3 cut(s) 1416, 1656, 2169
FspBI CTAG 3 cut(s) 818, 1884, 2370
GluI GCNGC 3 cut(s) 1416, 1656, 2169
GsuI CTGGAG 3 cut(s) 318, 534, 1984
HaeIII GGCC 6 cut(s) 14, 122, 588, 802, 1233, 2620
HapII CCGG 2 cut(s) 236, 1948
HincII GTYRAC 4 cut(s) 366, 867, 2524, 2722
HindII GTYRAC 4 cut(s) 366, 867, 2524, 2722
HindIII AAGCTT 4 cut(s) 323, 332, 619, 905
HpaI GTTAAC 1 cut(s) 2722
HpaII CCGG 2 cut(s) 236, 1948
HphI GGTGA 6 cut(s) 455, 968, 1548, 2183, 2533, 2825
Hpy166II GTNNAC 8 cut(s) 366, 867, 1894, 1904, 2455, 2524, 2595, 2722
Hpy188I TCNGA 9 cut(s) 283, 429, 651, 1390, 2278, 2398, 2481, 2614, 2788
Hpy8I GTNNAC 8 cut(s) 366, 867, 1894, 1904, 2455, 2524, 2595, 2722
HpyAV CCTTC 5 cut(s) 319, 1007, 1162, 1802, 2440
HpyCH4III ACNGT 7 cut(s) 254, 614, 1475, 1891, 2043, 2509, 2521
HpyCH4IV ACGT 3 cut(s) 1065, 1549, 2146
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 331, 967, 2165, 2208, 2428, 2626, 2767
HpyF3I CTNAG 6 cut(s) 738, 908, 1331, 1387, 1842, 2613
HpySE526I ACGT 3 cut(s) 1065, 1549, 2146
Ksp22I TGATCA 1 cut(s) 1459
KspAI GTTAAC 1 cut(s) 2722
LmnI GCTCC 3 cut(s) 515, 1377, 2165
Lsp1109I GCAGC 2 cut(s) 1667, 2180
LweI GCATC 4 cut(s) 46, 2330, 2482, 2779
MaeI CTAG 3 cut(s) 818, 1884, 2370
MaeII ACGT 3 cut(s) 1065, 1549, 2146
MaeIII GTNAC 5 cut(s) 98, 857, 1592, 2348, 2539
MboII GAAGA 6 cut(s) 80, 364, 2056, 2180, 2455, 2795
MfeI CAATTG 1 cut(s) 2343
MflI RGATCY 3 cut(s) 627, 1088, 1751
MhlI GDGCHC 3 cut(s) 520, 1266, 2597
MlyI GAGTC 2 cut(s) 1932, 2471
MmeI TCCRAC 2 cut(s) 1749, 2143
Mph1103I ATGCAT 2 cut(s) 2323, 2631
MroXI GAANNNNTTC 1 cut(s) 1058
MslI CAYNNNNRTG 5 cut(s) 1299, 1352, 1802, 2223, 2546
MspA1I CMGCKG 1 cut(s) 1582
MspCI CTTAAG 1 cut(s) 326
MspI CCGG 2 cut(s) 236, 1948
MspR9I CCNGG 4 cut(s) 236, 632, 954, 1636
MunI CAATTG 1 cut(s) 2343
Mva1269I GAATGC 1 cut(s) 1380
MvaI CCWGG 3 cut(s) 632, 954, 1636
MwoI GCNNNNNNNGC 8 cut(s) 11, 331, 967, 2165, 2208, 2428, 2626, 2767
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 1 cut(s) 1698
NlaIV GGNNCC 5 cut(s) 431, 1018, 1039, 1090, 1328
NmeAIII GCCGAG 1 cut(s) 2359
NmuCI GTSAC 3 cut(s) 98, 1592, 2539
NsiI ATGCAT 2 cut(s) 2323, 2631
PagI TCATGA 1 cut(s) 61
PaqCI CACCTGC 1 cut(s) 2552
PceI AGGCCT 1 cut(s) 14
PctI GAATGC 1 cut(s) 1380
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 8 cut(s) 145, 194, 664, 733, 991, 1291, 1836, 1846
PflFI GACNNNGTC 2 cut(s) 104, 1770
PflMI CCANNNNNTGG 1 cut(s) 173
PfoI TCCNGGA 2 cut(s) 234, 630
PinAI ACCGGT 1 cut(s) 1947
PkrI GCNGC 3 cut(s) 1417, 1657, 2170
PleI GAGTC 2 cut(s) 1932, 2470
PpsI GAGTC 2 cut(s) 1932, 2470
PpuMI RGGWCCY 3 cut(s) 1016, 1327, 1772
PshBI ATTAAT 1 cut(s) 1167
Psp124BI GAGCTC 1 cut(s) 520
Psp1406I AACGTT 1 cut(s) 1065
Psp5II RGGWCCY 3 cut(s) 1016, 1327, 1772
Psp6I CCWGG 3 cut(s) 630, 952, 1634
PspGI CCWGG 3 cut(s) 630, 952, 1634
PspN4I GGNNCC 5 cut(s) 431, 1018, 1039, 1090, 1328
PspPI GGNCC 9 cut(s) 371, 587, 800, 1016, 1232, 1304, 1327, 1772, 2111
PspPPI RGGWCCY 3 cut(s) 1016, 1327, 1772
PstI CTGCAG 2 cut(s) 1119, 1318
PstNI CAGNNNCTG 1 cut(s) 1596
PsuI RGATCY 3 cut(s) 627, 1088, 1751
PsyI GACNNNGTC 2 cut(s) 104, 1770
RsaI GTAC 6 cut(s) 485, 557, 616, 1527, 1895, 1905
RsaNI GTAC 6 cut(s) 484, 556, 615, 1526, 1894, 1904
RseI CAYNNNNRTG 5 cut(s) 1299, 1352, 1802, 2223, 2546
SacI GAGCTC 1 cut(s) 520
SatI GCNGC 3 cut(s) 1416, 1656, 2169
Sau96I GGNCC 9 cut(s) 371, 587, 800, 1016, 1232, 1304, 1327, 1772, 2111
SchI GAGTC 2 cut(s) 1932, 2471
ScrFI CCNGG 4 cut(s) 236, 632, 954, 1636
SduI GDGCHC 3 cut(s) 520, 1266, 2597
SfaNI GCATC 4 cut(s) 46, 2330, 2482, 2779
SfcI CTRYAG 2 cut(s) 1115, 1314
SinI GGWCC 6 cut(s) 371, 1016, 1304, 1327, 1772, 2111
SmiMI CAYNNNNRTG 5 cut(s) 1299, 1352, 1802, 2223, 2546
SmlI CTYRAG 7 cut(s) 326, 335, 1172, 1259, 1484, 2172, 2426
SmoI CTYRAG 7 cut(s) 326, 335, 1172, 1259, 1484, 2172, 2426
SseBI AGGCCT 1 cut(s) 14
SsiI CCGC 7 cut(s) 153, 1078, 1415, 1580, 2209, 2309, 2717
SspMI CTAG 3 cut(s) 818, 1884, 2370
SstI GAGCTC 1 cut(s) 520
StuI AGGCCT 1 cut(s) 14
StyD4I CCNGG 4 cut(s) 234, 630, 952, 1634
StyI CCWWGG 6 cut(s) 172, 452, 487, 1698, 2049, 2490
TaaI ACNGT 7 cut(s) 254, 614, 1475, 1891, 2043, 2509, 2521
TaiI ACGT 3 cut(s) 1068, 1552, 2149
TaqI TCGA 5 cut(s) 1607, 2117, 2324, 2355, 2700
TatI WGTACW 3 cut(s) 614, 1893, 1903
TauI GCSGC 1 cut(s) 1418
TfiI GAWTC 8 cut(s) 145, 194, 664, 733, 991, 1291, 1836, 1846
TseFI GTSAC 3 cut(s) 98, 1592, 2539
TseI GCWGC 2 cut(s) 1655, 2168
Tsp45I GTSAC 3 cut(s) 98, 1592, 2539
TspGWI ACGGA 2 cut(s) 1779, 1994
Tth111I GACNNNGTC 2 cut(s) 104, 1770
Van91I CCANNNNNTGG 1 cut(s) 173
Vha464I CTTAAG 1 cut(s) 326
VneI GTGCAC 1 cut(s) 2593
VpaK11BI GGWCC 6 cut(s) 371, 1016, 1304, 1327, 1772, 2111
VspI ATTAAT 1 cut(s) 1167
XagI CCTNNNNNAGG 1 cut(s) 2614
XcmI CCANNNNNNNNNTGG 3 cut(s) 40, 145, 1006
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 3 cut(s) 818, 1884, 2370
Zsp2I ATGCAT 2 cut(s) 2323, 2631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.