Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002290

Physical Location & Seq

Genomic Coordinates Forward (+)

21713245 .. 21717878

Gene Structure 4634 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G53480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGCAATGGAGGTTACGCAGTTGCTCATTAATGCTCAGTCGATTGATGGAACTGTACGTAAGCACGCGGAAGAAAGTCTTAAGCAGTTTCAGGAGCAAAACCTTGCGGGTTTCTTGTTGTCGCTTGCTGGAGAGCTTGCGAATGATGAGAAGCCAGTAGATAGCAGGAAATTAGCTGGTTTAGTCCTTAAAAATGCTCTTGATGCTAAGGAACAACACAGGAAGTATGAGCTTGTTCAGAGATGGTTGGCTCTAGACATGTCAACAAAGTCGCAGATCAGAGCTTTCTTGTTGAAGACACTGTCGGCACCTGTGCCTGATGTTCGTTCGACTGCATCTCAGGTCATTGCTAAGGTTGCAGGTATTGAGTTACCACAGAAGCAGTGGCCTGAGCTCATAGTGTCTCTTCTCTCAAATATTCACCAGTTACCTGCTCATGTCAAGCAAGCCACTTTGGAGACTCTTGGATACCTATGTGAAGAAGTGTCACCTGATGTTGTTGAACAGGAGCATGTAAATAAGATACTCACAGCTGTTGTTCAGGGTATGAATGCTGCTGAAGGTAATACTGATGTTAGACTTGCTGCAACCCGTGCTTTATACATGGCTCTCGGATTCGCCCAAGCAAATTTCAACAACGACATGGAGCGTGATTATATCATGAGAGTTGTGTGTGAAGCAACCCTGTCCCCTGAGGTGAAAATTAGGCAGGCAGCTTTTGAGTGTTTGGTATCTATCGCTTCCACATACTATGAGAAGTTGGCGCATTACATGCAAGATATATTCAACATCACAGCTAAGGCTGTAAGAGAAGATGACGAGTCTGTTGCTCTACAGGCAATTGAGTTCTGGAGTTCTATTTGTGACGAAGAGATTGACATCTTAGAAGAATATGGTGGTGAGTTCGCTGGGGATTCTGATGTTCCATGCTTTTATTTTACTAAGCAGGCTCTCCCTGGTCTTGTGCCTCTACTGCTGGAGACTCTTCTGAAGCAAGAAGAAGATCAAGATTTGGATGAAGGGGCTTGGAATATTGCAATGGCAGGTGGGACATGCCTCGGTTTGGTTGCTAGGGCAGTTGGAGATGACATTGTTCCACATGTCATGCCGTTTATTGAAGAGAAAATATCAAAGCCTGATTGGAGAGAGCGAGAAGCTGCAACTTATGCTTTTGGTTCCATTTTGGAAGGCCCTTCTGCCGATAAGTTGATGGCAATTGTTAACGCAGCGTTAACATTTATGCTCAATGCTCTAACAAATGACCCAAGCAACCATGTGAAAGACACAACCGCATGGACCCTTGGTCGGATATTTGAGTTCCTTCATGGTTCAACAATCGAGACACCTATTATTAATCAGGCAAACTGCCAGCAGATAATCACAGTACTCATCCAGAGCATGAATGATGCGCCTAATGTTGCCGAGAAGGCTTGTGGGGCTCTGTACTTCCTTGCTCAAGGCTATGAGGATATCGGTCCCAGTTCTCCGTTAACACCCTTCTTCCAGGAAATTATTAAGTCACTTTTAGCTGTTGCACACAGAGAGGATGCAACTGAATCACGCTTGCGGACTGCAGCATATGAGGCATTGAATGAAGTTGTCAGGTGTTCAACTGATGAAACGTCTACCATGGTTCTGCAATTAGTACCAGTGATAATGATGGAGCTTCACAATACTTTGGAAGGGGAAAAGCTTTCATTGGATGAGAGGGAGAAACAAAACGAGTTGCAGGGACTTCTATGTGGATGCTTGCAGGTCATCATACAGAAATTAGGATCTGAACCAACCAAGTCTAAGTTCATGGAGTATGCAGACCAAATGATGGGACTTTTCCTGAGGGTGTTTGGTTGTAGAAGTGCAACTGCACATGAGGAAGCCATGCTTGCCATTGGTGCTCTTGCTTATGCAGCAGGTCCCAATTTTGCCAAATACATGCCTGAGTTTTATAAGTACTTGGAGATGGGTCTTCAAAACTTTGAAGAATACCAAGTGTGTGCTGTTACTGTTGGTGTTGTTGGGGATGTCTGCAGAGCATTGGAGGACAAGATTTTACCTTATTGCGATGGGATTATGACACAGCTTCTGAAAGATTTGTCGAGCAACCAATTGCACCGATCAGTGAAGCCACCGATATTCTCCTGTTTTGGTGACATAGCACTTGCTATCGGTGAGGATTTTGATAAGTACTGGAGGTATTCAATGCCGATGCTTCAGAGTGCCGCAGAGTTATCTGCTCACTCGGCTGGAGCTGATGATGAAATGACGGAGTACACAAACTCATTGAGAAACGGAATCCTTGAGGCTTATTCAGGCATATTTCAAGGTTTCAAGAACTCCGCCAAAACCCAGCTCCTGATTCCTTTCGCACCCCATATCCTCCAGTTTTTGGACAGTATATACATGGAGAAAGACATGGACGAGGTGGTGATGAAGACAGCGATTGGGGTCTTAGGAGATTTAGCAGATACACTAGGGAGTCATGTGGGTGGTTTGATACAACAGTCAGTGTCAAGTAAAGAGTTCTTAAACGAATGTTTGTCTTCTGAAGACCACACAATTAAAGAAGCAGCCGAATGGGCGAAGCATGCCATAACCCGTGCCATATCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009507 GO:0009536 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N

Protein Analysis

870

Amino Acids

96.26

Weight (kDa)

4.71

Isoelectric Point (pI)

44.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	23 - 101	1.7e-12	Importin-beta N-terminal domain
HEAT_EZ	PF13513	381 - 437	2e-11	HEAT-like repeat
TPR_IMB1	PF25574	459 - 825	1.1e-108	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002290)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53480
fragaria_vesca	FvH4_5g11510 FvH4_5g11510 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050
malus_domestica	MD01G1223400.v1.1 MD06G1112600.v1.1 MD07G1294100.v1.1 MD14G1134000.v1.1
prunus_persica	Prupe.2G313900_v2.0.a1 Prupe.5G125800_v2.0.a1
pyrus_communis	pycom01g23260 pycom06g10550 pycom07g26690
rosa_chinensis	RchiOBHm_Chr1g0381661 RchiOBHm_Chr7g0186291
rosa_laevigata	RLG00000004852 RLG00000026217
rosa_multiflora	Rmu_sc0000645.1_g000053 Rmu_sc0012555.1_g000004
rosa_roxburghii	Rroxscaffold_3G00268280
rosa_rugosa	Rorug01G0430300 Rorug01G0430300 Rorug01G0430300 Rorug01G0430300 Rorug01G0430300 Rorug06G0473100 Rorug06G0473100
rosa_samantha	Rh1AG453500 Rh1BG410700 Rh1CG425100 Rh1DG442400 Rh7AG068000 Rh7CG080000 Rh7DG080600
rosa_wichuraiana	Rw1G039560 Rw7G006700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1947
AarI	CACCTGC	1 cut(s)	1034
Acc36I	ACCTGC	5 cut(s)	350, 439, 1034, 1744, 1901
AccB1I	GGYRCC	1 cut(s)	307
AccB7I	CCANNNNNTGG	2 cut(s)	1822, 2386
AccI	GTMKAC	1 cut(s)	1625
AccII	CGCG	1 cut(s)	68
AciI	CCGC	6 cut(s)	68, 107, 1290, 1567, 2220, 2337
AclWI	GGATC	1 cut(s)	1783
AcsI	RAATTY	1 cut(s)	628
AcuI	CTGAAG	4 cut(s)	579, 1010, 2195, 2565
AfaI	GTAC	7 cut(s)	57, 1386, 1445, 1647, 1952, 2186, 2270
AfiI	CCNNNNNNNGG	4 cut(s)	1063, 1305, 1822, 2386
AflII	CTTAAG	1 cut(s)	80
AflIII	ACRYGT	2 cut(s)	258, 1099
AhdI	GACNNNNNGTC	1 cut(s)	1302
AjnI	CCWGG	2 cut(s)	955, 1503
AjuI	GAANNNNNNNTTGG	2 cut(s)	1781, 1813
Alw21I	GWGCWC	2 cut(s)	396, 1897
Alw26I	GTCTC	4 cut(s)	408, 452, 974, 1334
AlwI	GGATC	1 cut(s)	1783
AlwNI	CAGNNNCTG	2 cut(s)	2083, 2353
AoxI	GGCC	2 cut(s)	386, 1189
ApeKI	GCWGC	8 cut(s)	554, 584, 713, 1157, 1226, 1574, 1907, 2567
ApoI	RAATTY	1 cut(s)	628
AseI	ATTAAT	2 cut(s)	30, 1353
AspLEI	GCGC	2 cut(s)	766, 1411
AspS9I	GGNCC	4 cut(s)	1190, 1296, 1475, 1913
AsuHPI	GGTGA	7 cut(s)	413, 480, 709, 911, 2159, 2180, 2437
AvaII	GGWCC	3 cut(s)	1296, 1475, 1913
AxyI	CCTNAGG	2 cut(s)	693, 1835
BaeI	ACNNNNGTAYC	4 cut(s)	460, 493, 2486, 2519
BanI	GGYRCC	1 cut(s)	307
BanII	GRGCYC	2 cut(s)	396, 1441
BbsI	GAAGAC	5 cut(s)	302, 1958, 2438, 2532, 2553
Bbv12I	GWGCWC	2 cut(s)	396, 1897
BbvI	GCAGC	8 cut(s)	541, 571, 725, 1144, 1238, 1586, 1919, 2579
BccI	CCATC	7 cut(s)	41, 237, 1204, 1654, 1816, 1954, 2057
BceAI	ACGGC	1 cut(s)	1093
BcgI	CGANNNNNNTGC	2 cut(s)	809, 843
BciT130I	CCWGG	2 cut(s)	957, 1505
BciVI	GTATCC	1 cut(s)	461
BcoDI	GTCTC	4 cut(s)	408, 452, 974, 1334
BfaI	CTAG	3 cut(s)	254, 1071, 2471
BfmI	CTRYAG	3 cut(s)	833, 1572, 2026
BfrI	CTTAAG	1 cut(s)	80
BfuAI	ACCTGC	5 cut(s)	350, 439, 1034, 1744, 1901
BfuI	GTATCC	1 cut(s)	461
BglI	GCCNNNNNGGC	2 cut(s)	1427, 2576
BisI	GCNGC	9 cut(s)	555, 585, 714, 1158, 1227, 1575, 1908, 2220, 2568
BlsI	GCNGC	9 cut(s)	556, 586, 715, 1159, 1228, 1576, 1909, 2221, 2569
BmcAI	AGTACT	3 cut(s)	1386, 1952, 2186
Bme1390I	CCNGG	2 cut(s)	957, 1505
Bme18I	GGWCC	3 cut(s)	1296, 1475, 1913
BmeRI	GACNNNNNGTC	1 cut(s)	1302
BmgT120I	GGNCC	4 cut(s)	1190, 1296, 1475, 1913
BmiI	GGNNCC	5 cut(s)	309, 1177, 1298, 1477, 1915
BmrFI	CCNGG	2 cut(s)	957, 1505
BmrI	ACTGGG	1 cut(s)	1473
BmsI	GCATC	6 cut(s)	193, 344, 1396, 1537, 1736, 2196
BmuI	ACTGGG	1 cut(s)	1473
BpiI	GAAGAC	5 cut(s)	302, 1958, 2438, 2532, 2553
BpmI	CTGGAG	6 cut(s)	150, 871, 998, 2209, 2265, 2363
Bpu10I	CCTNAGC	4 cut(s)	207, 351, 390, 798
BpuEI	CTTGAG	2 cut(s)	1440, 2318
BsaAI	YACGTR	1 cut(s)	59
BsaBI	GATNNNNATC	1 cut(s)	878
BsaJI	CCNNGG	4 cut(s)	955, 1057, 1300, 1629
Bsc4I	CCNNNNNNNGG	4 cut(s)	1063, 1305, 1822, 2386
Bse1I	ACTGG	6 cut(s)	155, 424, 1479, 1649, 2192, 2380
Bse21I	CCTNAGG	2 cut(s)	693, 1835
Bse3DI	GCAATG	3 cut(s)	12, 345, 1044
Bse8I	GATNNNNATC	1 cut(s)	878
BseBI	CCWGG	2 cut(s)	957, 1505
BseDI	CCNNGG	4 cut(s)	955, 1057, 1300, 1629
BseGI	GGATG	6 cut(s)	1021, 1389, 1552, 1708, 1751, 2026
BseJI	GATNNNNATC	1 cut(s)	878
BseLI	CCNNNNNNNGG	4 cut(s)	1063, 1305, 1822, 2386
BseMI	GCAATG	3 cut(s)	12, 345, 1044
BseMII	CTCAG	6 cut(s)	50, 353, 381, 684, 1826, 1929
BseNI	ACTGG	6 cut(s)	155, 424, 1479, 1649, 2192, 2380
BseXI	GCAGC	8 cut(s)	541, 571, 725, 1144, 1238, 1586, 1919, 2579
BseYI	CCCAGC	2 cut(s)	908, 2346
BsgI	GTGCAG	1 cut(s)	1848
Bsh1236I	CGCG	1 cut(s)	68
BshFI	GGCC	2 cut(s)	388, 1191
BshNI	GGYRCC	1 cut(s)	307
BsiHKAI	GWGCWC	2 cut(s)	396, 1897
BslFI	GGGAC	6 cut(s)	673, 1063, 1461, 1746, 1839, 1899
BslI	CCNNNNNNNGG	4 cut(s)	1063, 1305, 1822, 2386
BsmAI	GTCTC	4 cut(s)	408, 452, 974, 1334
BsmFI	GGGAC	6 cut(s)	673, 1063, 1461, 1746, 1839, 1899
BsmI	GAATGC	1 cut(s)	556
BsnI	GGCC	2 cut(s)	388, 1191
Bsp1286I	GDGCHC	3 cut(s)	396, 1441, 1897
Bsp143I	GATC	4 cut(s)	276, 1003, 1775, 2114
Bsp19I	CCATGG	1 cut(s)	1629
BspACI	CCGC	6 cut(s)	68, 107, 1290, 1567, 2220, 2337
BspANI	GGCC	2 cut(s)	388, 1191
BspCNI	CTCAG	6 cut(s)	49, 352, 382, 685, 1827, 1930
BspFNI	CGCG	1 cut(s)	68
BspHI	TCATGA	1 cut(s)	660
BspLI	GGNNCC	5 cut(s)	309, 1177, 1298, 1477, 1915
BspMAI	CTGCAG	2 cut(s)	1576, 2030
BspMI	ACCTGC	5 cut(s)	350, 439, 1034, 1744, 1901
BspPI	GGATC	1 cut(s)	1783
BspT107I	GGYRCC	1 cut(s)	307
BspTI	CTTAAG	1 cut(s)	80
BsrDI	GCAATG	3 cut(s)	12, 345, 1044
BsrI	ACTGG	6 cut(s)	155, 424, 1479, 1649, 2192, 2380
BssECI	CCNNGG	4 cut(s)	955, 1057, 1300, 1629
BssMI	GATC	4 cut(s)	276, 1003, 1775, 2114
BssT1I	CCWWGG	2 cut(s)	1300, 1629
Bst2UI	CCWGG	2 cut(s)	957, 1505
Bst4CI	ACNGT	6 cut(s)	55, 303, 1384, 2005, 2393, 2502
Bst6I	CTCTTC	4 cut(s)	411, 864, 990, 1113
BstAFI	CTTAAG	1 cut(s)	80
BstAPI	GCANNNNNTGC	3 cut(s)	593, 772, 1166
BstBAI	YACGTR	1 cut(s)	59
BstDSI	CCRYGG	1 cut(s)	1629
BstF5I	GGATG	6 cut(s)	1021, 1389, 1552, 1708, 1751, 2026
BstFNI	CGCG	1 cut(s)	68
BstHHI	GCGC	2 cut(s)	766, 1411
BstKTI	GATC	4 cut(s)	279, 1006, 1778, 2117
BstMAI	GTCTC	4 cut(s)	408, 452, 974, 1334
BstMBI	GATC	4 cut(s)	276, 1003, 1775, 2114
BstNI	CCWGG	2 cut(s)	957, 1505
BstNSI	RCATGY	7 cut(s)	262, 515, 775, 1056, 1103, 1936, 2588
BstSCI	CCNGG	2 cut(s)	955, 1503
BstSFI	CTRYAG	3 cut(s)	833, 1572, 2026
BstSNI	TACGTA	1 cut(s)	59
BstUI	CGCG	1 cut(s)	68
BstV1I	GCAGC	8 cut(s)	541, 571, 725, 1144, 1238, 1586, 1919, 2579
BstV2I	GAAGAC	5 cut(s)	302, 1958, 2438, 2532, 2553
BstX2I	RGATCY	1 cut(s)	1775
BstYI	RGATCY	1 cut(s)	1775
Bsu36I	CCTNAGG	2 cut(s)	693, 1835
BsuI	GTATCC	1 cut(s)	461
BsuRI	GGCC	2 cut(s)	388, 1191
BtgI	CCRYGG	1 cut(s)	1629
BtgZI	GCGATG	1 cut(s)	2076
BtsCI	GGATG	6 cut(s)	1021, 1389, 1552, 1708, 1751, 2026
BtsI	GCAGTG	1 cut(s)	389
BtsIMutI	CAGTG	5 cut(s)	299, 389, 1656, 2124, 2511
BveI	ACCTGC	5 cut(s)	350, 439, 1034, 1744, 1901
CaiI	CAGNNNCTG	2 cut(s)	2083, 2353
CciI	TCATGA	1 cut(s)	660
CfoI	GCGC	2 cut(s)	766, 1411
Cfr13I	GGNCC	4 cut(s)	1190, 1296, 1475, 1913
Csp6I	GTAC	7 cut(s)	56, 1385, 1444, 1646, 1951, 2185, 2269
CviQI	GTAC	7 cut(s)	56, 1385, 1444, 1646, 1951, 2185, 2269
DpnI	GATC	4 cut(s)	278, 1005, 1777, 2116
DpnII	GATC	4 cut(s)	276, 1003, 1775, 2114
DriI	GACNNNNNGTC	1 cut(s)	1302
Eam1104I	CTCTTC	4 cut(s)	411, 864, 990, 1113
Eam1105I	GACNNNNNGTC	1 cut(s)	1302
EarI	CTCTTC	4 cut(s)	411, 864, 990, 1113
EciI	GGCGGA	1 cut(s)	2326
Ecl136II	GAGCTC	1 cut(s)	394
Eco105I	TACGTA	1 cut(s)	59
Eco130I	CCWWGG	2 cut(s)	1300, 1629
Eco24I	GRGCYC	2 cut(s)	396, 1441
Eco32I	GATATC	1 cut(s)	1471
Eco47I	GGWCC	3 cut(s)	1296, 1475, 1913
Eco53kI	GAGCTC	1 cut(s)	394
Eco57I	CTGAAG	4 cut(s)	579, 1010, 2195, 2565
Eco81I	CCTNAGG	2 cut(s)	693, 1835
EcoICRI	GAGCTC	1 cut(s)	394
EcoO109I	RGGNCCY	2 cut(s)	1190, 1913
EcoRII	CCWGG	2 cut(s)	955, 1503
EcoRV	GATATC	1 cut(s)	1471
EcoT14I	CCWWGG	2 cut(s)	1300, 1629
EcoT38I	GRGCYC	2 cut(s)	396, 1441
ErhI	CCWWGG	2 cut(s)	1300, 1629
FalI	AAGNNNNNCTT	4 cut(s)	63, 95, 1866, 1898
FaqI	GGGAC	6 cut(s)	673, 1063, 1461, 1746, 1839, 1899
FauI	CCCGC	1 cut(s)	100
FauNDI	CATATG	1 cut(s)	1579
FblI	GTMKAC	1 cut(s)	1625
Fnu4HI	GCNGC	9 cut(s)	555, 585, 714, 1158, 1227, 1575, 1908, 2220, 2568
FokI	GGATG	6 cut(s)	1028, 1376, 1559, 1715, 1758, 2033
FriOI	GRGCYC	2 cut(s)	396, 1441
Fsp4HI	GCNGC	9 cut(s)	555, 585, 714, 1158, 1227, 1575, 1908, 2220, 2568
FspBI	CTAG	3 cut(s)	254, 1071, 2471
GlaI	GCGC	2 cut(s)	765, 1410
GluI	GCNGC	9 cut(s)	555, 585, 714, 1158, 1227, 1575, 1908, 2220, 2568
GsaI	CCCAGC	2 cut(s)	912, 2350
GsuI	CTGGAG	6 cut(s)	150, 871, 998, 2209, 2265, 2363
HaeIII	GGCC	2 cut(s)	388, 1191
HhaI	GCGC	2 cut(s)	766, 1411
Hin6I	GCGC	2 cut(s)	764, 1409
HinP1I	GCGC	2 cut(s)	764, 1409
HincII	GTYRAC	4 cut(s)	264, 1222, 1233, 1491
HindII	GTYRAC	4 cut(s)	264, 1222, 1233, 1491
HindIII	AAGCTT	1 cut(s)	1691
HinfI	GANTC	9 cut(s)	460, 615, 821, 914, 982, 1556, 2292, 2356, 2476
HpaI	GTTAAC	3 cut(s)	1222, 1233, 1491
HphI	GGTGA	7 cut(s)	413, 480, 709, 911, 2159, 2180, 2437
Hpy166II	GTNNAC	6 cut(s)	264, 1222, 1233, 1491, 1626, 2271
Hpy8I	GTNNAC	6 cut(s)	264, 1222, 1233, 1491, 1626, 2271
HpyAV	CCTTC	8 cut(s)	554, 1013, 1181, 1203, 1331, 1420, 1507, 1676
HpyCH4III	ACNGT	6 cut(s)	55, 303, 1384, 2005, 2393, 2502
HpyCH4IV	ACGT	2 cut(s)	58, 1622
HpySE526I	ACGT	2 cut(s)	58, 1622
HspAI	GCGC	2 cut(s)	764, 1409
KspAI	GTTAAC	3 cut(s)	1222, 1233, 1491
Kzo9I	GATC	4 cut(s)	276, 1003, 1775, 2114
LmnI	GCTCC	6 cut(s)	94, 508, 646, 1663, 2246, 2355
Lsp1109I	GCAGC	8 cut(s)	541, 571, 725, 1144, 1238, 1586, 1919, 2579
LweI	GCATC	6 cut(s)	193, 344, 1396, 1537, 1736, 2196
MaeI	CTAG	3 cut(s)	254, 1071, 2471
MaeII	ACGT	2 cut(s)	58, 1622
MaeIII	GTNAC	8 cut(s)	13, 369, 426, 486, 863, 1518, 1999, 2147
MalI	GATC	4 cut(s)	278, 1005, 1777, 2116
MboI	GATC	4 cut(s)	276, 1003, 1775, 2114
MfeI	CAATTG	3 cut(s)	840, 1215, 2105
MflI	RGATCY	1 cut(s)	1775
MhlI	GDGCHC	3 cut(s)	396, 1441, 1897
MlyI	GAGTC	4 cut(s)	454, 830, 976, 2485
MmeI	TCCRAC	2 cut(s)	1060, 1286
MslI	CAYNNNNRTG	1 cut(s)	2484
MspA1I	CMGCKG	1 cut(s)	533
MspCI	CTTAAG	1 cut(s)	80
MspR9I	CCNGG	2 cut(s)	957, 1505
MunI	CAATTG	3 cut(s)	840, 1215, 2105
Mva1269I	GAATGC	1 cut(s)	556
MvaI	CCWGG	2 cut(s)	957, 1505
MvnI	CGCG	1 cut(s)	68
NcoI	CCATGG	1 cut(s)	1629
NdeI	CATATG	1 cut(s)	1579
NdeII	GATC	4 cut(s)	276, 1003, 1775, 2114
NlaIV	GGNNCC	5 cut(s)	309, 1177, 1298, 1477, 1915
NmeAIII	GCCGAG	2 cut(s)	1447, 2219
NmuCI	GTSAC	4 cut(s)	486, 863, 1518, 2147
NspI	RCATGY	7 cut(s)	262, 515, 775, 1056, 1103, 1936, 2588
PaeI	GCATGC	1 cut(s)	2588
PagI	TCATGA	1 cut(s)	660
PaqCI	CACCTGC	1 cut(s)	1034
PciI	ACATGT	2 cut(s)	258, 1099
PctI	GAATGC	1 cut(s)	556
PfeI	GAWTC	5 cut(s)	615, 914, 1556, 2292, 2356
PflFI	GACNNNGTC	1 cut(s)	301
PflMI	CCANNNNNTGG	2 cut(s)	1822, 2386
PfoI	TCCNGGA	1 cut(s)	1503
PkrI	GCNGC	9 cut(s)	556, 586, 715, 1159, 1228, 1576, 1909, 2221, 2569
PleI	GAGTC	4 cut(s)	454, 829, 976, 2484
PpsI	GAGTC	4 cut(s)	454, 829, 976, 2484
Ppu21I	YACGTR	1 cut(s)	59
PpuMI	RGGWCCY	1 cut(s)	1913
PscI	ACATGT	2 cut(s)	258, 1099
PshBI	ATTAAT	2 cut(s)	30, 1353
PsiI	TTATAA	1 cut(s)	1947
Psp124BI	GAGCTC	1 cut(s)	396
Psp5II	RGGWCCY	1 cut(s)	1913
Psp6I	CCWGG	2 cut(s)	955, 1503
PspFI	CCCAGC	2 cut(s)	908, 2346
PspGI	CCWGG	2 cut(s)	955, 1503
PspN4I	GGNNCC	5 cut(s)	309, 1177, 1298, 1477, 1915
PspPI	GGNCC	4 cut(s)	1190, 1296, 1475, 1913
PspPPI	RGGWCCY	1 cut(s)	1913
PstI	CTGCAG	2 cut(s)	1576, 2030
PstNI	CAGNNNCTG	2 cut(s)	2083, 2353
PsuI	RGATCY	1 cut(s)	1775
PsyI	GACNNNGTC	1 cut(s)	301
PvuII	CAGCTG	1 cut(s)	533
RsaI	GTAC	7 cut(s)	57, 1386, 1445, 1647, 1952, 2186, 2270
RsaNI	GTAC	7 cut(s)	56, 1385, 1444, 1646, 1951, 2185, 2269
RseI	CAYNNNNRTG	1 cut(s)	2484
SacI	GAGCTC	1 cut(s)	396
SatI	GCNGC	9 cut(s)	555, 585, 714, 1158, 1227, 1575, 1908, 2220, 2568
Sau3AI	GATC	4 cut(s)	276, 1003, 1775, 2114
Sau96I	GGNCC	4 cut(s)	1190, 1296, 1475, 1913
ScaI	AGTACT	3 cut(s)	1386, 1952, 2186
SchI	GAGTC	4 cut(s)	454, 830, 976, 2485
ScrFI	CCNGG	2 cut(s)	957, 1505
SduI	GDGCHC	3 cut(s)	396, 1441, 1897
SfaNI	GCATC	6 cut(s)	193, 344, 1396, 1537, 1736, 2196
SfcI	CTRYAG	3 cut(s)	833, 1572, 2026
SinI	GGWCC	3 cut(s)	1296, 1475, 1913
SmiMI	CAYNNNNRTG	1 cut(s)	2484
SmlI	CTYRAG	3 cut(s)	80, 1455, 2297
SmoI	CTYRAG	3 cut(s)	80, 1455, 2297
SnaBI	TACGTA	1 cut(s)	59
SphI	GCATGC	1 cut(s)	2588
SsiI	CCGC	6 cut(s)	68, 107, 1290, 1567, 2220, 2337
SspI	AATATT	2 cut(s)	418, 1033
SspMI	CTAG	3 cut(s)	254, 1071, 2471
SstI	GAGCTC	1 cut(s)	396
StyD4I	CCNGG	2 cut(s)	955, 1503
StyI	CCWWGG	2 cut(s)	1300, 1629
TaaI	ACNGT	6 cut(s)	55, 303, 1384, 2005, 2393, 2502
TaiI	ACGT	2 cut(s)	61, 1625
TaqI	TCGA	4 cut(s)	41, 329, 1338, 2096
TaqII	GACCGA	1 cut(s)	1463
TatI	WGTACW	5 cut(s)	1384, 1443, 1950, 2184, 2268
TauI	GCSGC	1 cut(s)	2222
TfiI	GAWTC	5 cut(s)	615, 914, 1556, 2292, 2356
TscAI	CASTG	5 cut(s)	306, 389, 1656, 2124, 2511
TseFI	GTSAC	4 cut(s)	486, 863, 1518, 2147
TseI	GCWGC	8 cut(s)	554, 584, 713, 1157, 1226, 1574, 1907, 2567
Tsp45I	GTSAC	4 cut(s)	486, 863, 1518, 2147
TspGWI	ACGGA	3 cut(s)	1476, 2279, 2304
TspRI	CASTG	5 cut(s)	306, 389, 1656, 2124, 2511
Tth111I	GACNNNGTC	1 cut(s)	301
Van91I	CCANNNNNTGG	2 cut(s)	1822, 2386
Vha464I	CTTAAG	1 cut(s)	80
VpaK11BI	GGWCC	3 cut(s)	1296, 1475, 1913
VspI	ATTAAT	2 cut(s)	30, 1353
XapI	RAATTY	1 cut(s)	628
XbaI	TCTAGA	1 cut(s)	253
XceI	RCATGY	7 cut(s)	262, 515, 775, 1056, 1103, 1936, 2588
XcmI	CCANNNNNNNNNTGG	1 cut(s)	381
XmiI	GTMKAC	1 cut(s)	1625
XspI	CTAG	3 cut(s)	254, 1071, 2471
ZrmI	AGTACT	3 cut(s)	1386, 1952, 2186

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.