MD01G1223400.v1.1

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002290
Physical Location & Seq
Reverse (-)
31513669 .. 31518580
4912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1223400.v1.1.491

Sequence Viewer

Length: 2631 bp
ATGCTGTTCCCATACTATACATCAATGGAAGTTACCCAGGTGCTTCTGAATGCTCAATCTATAGATGGAGCGGTACGGAATCATGCTGAAGAGAGTTTGAAACAGTTTCAGGAGCAAGATCTTTCATCGTTCTTGTTGTCTCTTTCTAGAGAGCTAGCAAATGAAGAAAAACCTGTTGAAAGCCGAAAATTAGCAGGTCTGATACTTAAGAATGCCCTGGATGCTAAAGAACAACCTAGAAAGTTGGAGCTTGTTCAAAGATGGCTTGCATTGGAAACTTCTGTGAAAGCGCAGATTAAGATGTTCTTGTTACAGATCCTCACTTCTCCTGTAGCTGATGCCCGTTCAACTACCTCTCAAGTTATTGCAAAGGTTGCGGGCATTGAGTTGCCCATGAAACAGTGGCCGGAACTGATTGGATCACTATTGTCAAATATTCACCGGTTACCAGCTCATGTAAAGCAAGCCACTTTAGAGACTCTTGGGTATTTGTGTGAGGAAATCTCTTCTGATGTCGTGGATCAAGATCAAGTGAATAAAATACTGACAGCTGTAGTTCAGGGTATGAACGCAACTGAAGGGAACAATGATGTTAGGCTTGCTGCTACCCGAGCACTGTACAATGCTTTGAATTTTGCTCAGGCCAATTTTAGCAATGACATGGAGCGTGATTATATTATGAGAGTTGTCTGTGAGGCAACTCTGTCCCCTGAAGTGAAGATTCGACAGGCAGCTTTTGAGTGTTTAGTTTCCATTTCTTCATCTTATTACGAGAAATTAGCCCCTTACATTCAGGACATATTTAGCATCACAGCAAAAGCTGTAAGGGAAGACCAGGAACCTGTTGCTCTTCAAGCCATTGAATTCTGGAGTTCGATTTGTGATGAGGAGATCGATATATTGGATGATTATGTGGGTGATTTTAGTGGGGACTCTGATATTCCTTGCTTCTACTTTATCAAGCAGGCAATCCCTGCCCTTGTCCCTATGCTTTTGGAGACGCTTCTTAAGCAGGAAGATGATCAGGAGGAAGATGGGGCTTGGAATATTGCTATGGCTGGGGGGACTTGCCTTGGGTTGGTTGCTCGGACAGTGGGAGATGATATCGTTCCACTTGTTATGCCTTTCATCGAAGAGAACATAACAAAACCAGATTGGAGGCAAAGAGAAGCAGCTACTTATGCGTTTGGTTCCATCTTGGAGGGTCCTTCGCCAAACAAGTTAACATCTATTGTCAATGTTGCTATGACGTTCATGCTCAGTGCTTTAACAAAGGACCCAAACAACCATGTGAAGGACACTACTGCCTGGACATTGGGAAGAATTTTTGAATTCCTTCATGGTTCAACTATGGATACACCCATAATTACCCCAGAAAACTGCCAACAGATCATCACAGTTCTTCTTCAGAGCATGAAAGATGTTCCAAATGTCGCTGAGAAGGCATGCGGTGCTCTCTATTTTCTGGCCCAGGGTTATGAGGATTTTGGCCCGTCATCTCCCATTGCTCCTTTTTTCCAGGAAATTGTACAGGCTCTACTCACTGTTACCCACAGACCAGATGCGGGGGAATCACGATTAAGGACTGCTGCATATGAAGCGTTGAATGAAGTCGTCAGATGTTCGACTGATGAAACTGCCCCCATGGTGTTGCAACTTATTCCCGTTATCATGATTGAACTGCATCAGACTCTTGAGGGCCAGCAGCTTGCATCTGATGAGAAAGAGAGGCAGAGTGAACTACAAGGACTTCTTTGTGGGTGTTTACAGGTCATTATTCAGAAACTAGGTTCATCAGAGCCCACAAAATATGTGTTCATGCCATACGCAGATCAGATAATGGGACTTTTCTTGAGGGTATTTGCTTGTAGAAGTGCAACTGTCCATGAGGAAGCCATGCTTGCCATTGGGGCCCTTGCATATACAAGTGGCCCGGATTTTGCAAAATACTTGCCTGAGTTTTACAAGTATCTCGAAATGGGGCTTCAAAATTTTGAGGAGTACCAAATCTGTGCTGTCACGGTTGGTGTTGTAGGAGATATTTGTAGAGCAGTGGAAGATAAGATTCTGCCTTATTGTGATGGAATTATGACCCAGCTTCTCAAGGATTTATCAAGCAACCAGTTGCACCGTTCCGTAAAGCCTCCTATATTCTCCTGCTTTGGTGATATAGCTCTTGCAATAGGAGAGAACTTTGATAAGTACTTGATGTACGCCATGCCAATGATTCAGAGTGCTGCAGAGATGTCTGCTCACACAGCAAGTGCTGATGATGTAATGACAGAGTACACCAATTCTCTGAGAAACGGAATCTTGGAGGCATATTCTGGGATTTTCCAAGGTTTCAAGAACTCCCCGAAAACCCAGGTTTTGATATCTTATGCTCCACACATCCTGCAGTTCTTGGATAGCTTATACAAGAGGAAAGATATGGATGAAGTGGTGATGAAAACAGCCATTGGTGTCCTTGGAGATCTAGCAGATACACTGGGAAATAATGCTGGTTCTTTGATACAGCAATCTCAATCGTGCAGAGACTTTTTAAATGAGTGCTTGTCTTCCGAAGACAATTTGATTAAAGAATCTGCAGAATGGGCCAAGATGGCCATCAGTCGTGCCATTTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

877

Amino Acids

97.24

Weight (kDa)

4.63

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBN_N PF03810 29 - 108 5.8e-11 Importin-beta N-terminal domain
HEAT_GCN1 PF23271 105 - 274 8.3e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EZ PF13513 386 - 442 7.1e-11 HEAT-like repeat
TPR_IMB1 PF25574 464 - 843 3.4e-109 Importin subunit beta-1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 71
AciI CCGC 4 cut(s) 71, 377, 1449, 1565
AclWI GGATC 3 cut(s) 310, 427, 528
AcoI YGGCCR 2 cut(s) 404, 2604
AcsI RAATTY 5 cut(s) 631, 863, 1323, 1331, 1990
AcuI CTGAAG 4 cut(s) 108, 597, 732, 1391
AfaI GTAC 7 cut(s) 75, 620, 1530, 2003, 2204, 2213, 2288
AfiI CCNNNNNNNGG 3 cut(s) 1078, 1294, 1565
AflII CTTAAG 2 cut(s) 206, 1007
AgeI ACCGGT 1 cut(s) 441
AjnI CCWGG 7 cut(s) 36, 216, 834, 1307, 1470, 1518, 2364
Alw21I GWGCWC 2 cut(s) 616, 1456
Alw26I GTCTC 4 cut(s) 144, 470, 992, 2529
AlwI GGATC 3 cut(s) 310, 427, 528
Ama87I CYCGRG 1 cut(s) 609
AoxI GGCC 9 cut(s) 404, 642, 1467, 1489, 1699, 1911, 1930, 2595, 2604
ApaI GGGCCC 1 cut(s) 1915
ApeKI GCWGC 6 cut(s) 602, 731, 1172, 1589, 1705, 2237
ApoI RAATTY 5 cut(s) 631, 863, 1323, 1331, 1990
AsiGI ACCGGT 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 1335
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 9 cut(s) 1205, 1276, 1468, 1490, 1699, 1911, 1912, 1931, 2595
AsuC2I CCSGG 1 cut(s) 1934
AsuHPI GGTGA 4 cut(s) 431, 929, 2177, 2455
AsuNHI GCTAGC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 609
AvaII GGWCC 2 cut(s) 1205, 1276
BaeGI GKGCMC 1 cut(s) 1915
BalI TGGCCA 1 cut(s) 2606
BanII GRGCYC 2 cut(s) 1803, 1915
BbsI GAAGAC 3 cut(s) 837, 2550, 2571
Bbv12I GWGCWC 2 cut(s) 616, 1456
BbvI GCAGC 6 cut(s) 589, 743, 1184, 1576, 1717, 2224
BccI CCATC 7 cut(s) 59, 255, 1028, 1202, 2075, 2596, 2615
BciT130I CCWGG 7 cut(s) 38, 218, 836, 1309, 1472, 1520, 2366
BciVI GTATCC 1 cut(s) 1348
BclI TGATCA 1 cut(s) 1021
BcnI CCSGG 1 cut(s) 1934
BcoDI GTCTC 4 cut(s) 144, 470, 992, 2529
BfaI CTAG 5 cut(s) 147, 155, 237, 1787, 2477
BfmI CTRYAG 6 cut(s) 60, 330, 552, 2238, 2396, 2586
BfrI CTTAAG 2 cut(s) 206, 1007
BfuAI ACCTGC 1 cut(s) 185
BfuI GTATCC 1 cut(s) 1348
BglI GCCNNNNNGGC 2 cut(s) 1910, 2603
BglII AGATCT 2 cut(s) 118, 2473
BisI GCNGC 6 cut(s) 603, 732, 1173, 1590, 1706, 2238
BlsI GCNGC 6 cut(s) 604, 733, 1174, 1591, 1707, 2239
BmcAI AGTACT 1 cut(s) 2204
Bme1390I CCNGG 8 cut(s) 38, 218, 836, 1309, 1472, 1520, 1934, 2366
Bme18I GGWCC 2 cut(s) 1205, 1276
BmeT110I CYCGRG 1 cut(s) 609
BmgT120I GGNCC 9 cut(s) 1205, 1276, 1468, 1490, 1699, 1911, 1912, 1931, 2595
BmiI GGNNCC 6 cut(s) 840, 1192, 1206, 1278, 1912, 1913
BmrFI CCNGG 8 cut(s) 38, 218, 836, 1309, 1472, 1520, 1934, 2366
BmrI ACTGGG 1 cut(s) 2498
BmsI GCATC 6 cut(s) 211, 328, 816, 1552, 1693, 1721
BmtI GCTAGC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 2498
BpiI GAAGAC 3 cut(s) 837, 2550, 2571
BplI GAGNNNNNCTC 2 cut(s) 488, 520
BpmI CTGGAG 1 cut(s) 889
Bpu10I CCTNAGC 1 cut(s) 639
BpuEI CTTGAG 4 cut(s) 342, 1715, 1873, 2087
BpuMI CCSGG 1 cut(s) 1934
Bsa29I ATCGAT 1 cut(s) 894
BsaBI GATNNNNATC 2 cut(s) 525, 2606
BsaJI CCNNGG 9 cut(s) 36, 216, 1072, 1470, 1471, 1644, 2338, 2364, 2467
BsaWI WCCGGW 1 cut(s) 441
Bsc4I CCNNNNNNNGG 3 cut(s) 1078, 1294, 1565
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 2 cut(s) 2122, 2493
Bse3DI GCAATG 2 cut(s) 661, 1503
Bse8I GATNNNNATC 2 cut(s) 525, 2606
BseBI CCWGG 7 cut(s) 38, 218, 836, 1309, 1472, 1520, 2366
BseCI ATCGAT 1 cut(s) 894
BseDI CCNNGG 9 cut(s) 36, 216, 1072, 1470, 1471, 1644, 2338, 2364, 2467
BseGI GGATG 4 cut(s) 226, 910, 2391, 2440
BseJI GATNNNNATC 2 cut(s) 525, 2606
BseLI CCNNNNNNNGG 3 cut(s) 1078, 1294, 1565
BseMI GCAATG 2 cut(s) 661, 1503
BseMII CTCAG 5 cut(s) 653, 1273, 1428, 1947, 2291
BseNI ACTGG 2 cut(s) 2122, 2493
BseRI GAGGAG 2 cut(s) 902, 2012
BseSI GKGCMC 1 cut(s) 1915
BseXI GCAGC 6 cut(s) 589, 743, 1184, 1576, 1717, 2224
BseYI CCCAGC 2 cut(s) 1058, 2094
BsgI GTGCAG 1 cut(s) 2551
BshFI GGCC 9 cut(s) 406, 644, 1469, 1491, 1701, 1913, 1932, 2597, 2606
BshTI ACCGGT 1 cut(s) 441
BshVI ATCGAT 1 cut(s) 894
BsiHKAI GWGCWC 2 cut(s) 616, 1456
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 3 cut(s) 407, 442, 1934
BslFI GGGAC 5 cut(s) 691, 944, 968, 1078, 1857
BslI CCNNNNNNNGG 3 cut(s) 1078, 1294, 1565
BsmAI GTCTC 4 cut(s) 144, 470, 992, 2529
BsmBI CGTCTC 1 cut(s) 992
BsmFI GGGAC 5 cut(s) 691, 944, 968, 1078, 1857
BsmI GAATGC 2 cut(s) 55, 217
BsnI GGCC 9 cut(s) 406, 644, 1469, 1491, 1701, 1913, 1932, 2597, 2606
BsoBI CYCGRG 1 cut(s) 609
Bsp120I GGGCCC 1 cut(s) 1911
Bsp1286I GDGCHC 4 cut(s) 616, 1456, 1803, 1915
Bsp1407I TGTACA 2 cut(s) 618, 1528
Bsp19I CCATGG 1 cut(s) 1644
BspACI CCGC 4 cut(s) 71, 377, 1449, 1565
BspANI GGCC 9 cut(s) 406, 644, 1469, 1491, 1701, 1913, 1932, 2597, 2606
BspCNI CTCAG 5 cut(s) 652, 1272, 1429, 1948, 2292
BspDI ATCGAT 1 cut(s) 894
BspHI TCATGA 1 cut(s) 1671
BspLI GGNNCC 6 cut(s) 840, 1192, 1206, 1278, 1912, 1913
BspMAI CTGCAG 3 cut(s) 2242, 2400, 2590
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 1 cut(s) 158
BspPI GGATC 3 cut(s) 310, 427, 528
BspQI GCTCTTC 1 cut(s) 855
BspTI CTTAAG 2 cut(s) 206, 1007
BsrBI CCGCTC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 661, 1503
BsrFI RCCGGY 1 cut(s) 441
BsrGI TGTACA 2 cut(s) 618, 1528
BsrI ACTGG 2 cut(s) 2122, 2493
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 9 cut(s) 36, 216, 1072, 1470, 1471, 1644, 2338, 2364, 2467
BssT1I CCWWGG 4 cut(s) 1072, 1644, 2338, 2467
Bst2UI CCWGG 7 cut(s) 38, 218, 836, 1309, 1472, 1520, 2366
Bst4CI ACNGT 9 cut(s) 105, 402, 618, 1093, 1399, 1546, 1882, 2023, 2132
Bst6I CTCTTC 4 cut(s) 84, 511, 855, 1128
BstAFI CTTAAG 2 cut(s) 206, 1007
BstAPI GCANNNNNTGC 3 cut(s) 374, 974, 1451
BstAUI TGTACA 2 cut(s) 618, 1528
BstDEI CTNAG 5 cut(s) 639, 1259, 1437, 1956, 2300
BstDSI CCRYGG 1 cut(s) 1644
BstEII GGTNACC 1 cut(s) 444
BstF5I GGATG 4 cut(s) 226, 910, 2391, 2440
BstHHI GCGC 1 cut(s) 292
BstMAI GTCTC 4 cut(s) 144, 470, 992, 2529
BstNI CCWGG 7 cut(s) 38, 218, 836, 1309, 1472, 1520, 2366
BstNSI RCATGY 1 cut(s) 1449
BstPI GGTNACC 1 cut(s) 444
BstSCI CCNGG 8 cut(s) 36, 216, 834, 1307, 1470, 1518, 1932, 2364
BstSFI CTRYAG 6 cut(s) 60, 330, 552, 2238, 2396, 2586
BstSLI GKGCMC 1 cut(s) 1915
BstV1I GCAGC 6 cut(s) 589, 743, 1184, 1576, 1717, 2224
BstV2I GAAGAC 3 cut(s) 837, 2550, 2571
BstX2I RGATCY 3 cut(s) 118, 315, 2473
BstYI RGATCY 3 cut(s) 118, 315, 2473
Bsu15I ATCGAT 1 cut(s) 894
BsuI GTATCC 1 cut(s) 1348
BsuRI GGCC 9 cut(s) 406, 644, 1469, 1491, 1701, 1913, 1932, 2597, 2606
BsuTUI ATCGAT 1 cut(s) 894
BtgI CCRYGG 1 cut(s) 1644
BtsCI GGATG 4 cut(s) 226, 910, 2391, 2440
BtsI GCAGTG 1 cut(s) 2058
BtsIMutI CAGTG 7 cut(s) 407, 614, 1098, 1267, 1542, 2058, 2486
BveI ACCTGC 1 cut(s) 185
CciI TCATGA 1 cut(s) 1671
CfoI GCGC 1 cut(s) 292
Cfr10I RCCGGY 1 cut(s) 441
Cfr13I GGNCC 9 cut(s) 1205, 1276, 1468, 1490, 1699, 1911, 1912, 1931, 2595
ClaI ATCGAT 1 cut(s) 894
CseI GACGC 1 cut(s) 1009
Csp6I GTAC 7 cut(s) 74, 619, 1529, 2002, 2203, 2212, 2287
CspAI ACCGGT 1 cut(s) 441
CviQI GTAC 7 cut(s) 74, 619, 1529, 2002, 2203, 2212, 2287
DdeI CTNAG 5 cut(s) 639, 1259, 1437, 1956, 2300
DraI TTTAAA 1 cut(s) 2544
EaeI YGGCCR 2 cut(s) 404, 2604
Eam1104I CTCTTC 4 cut(s) 84, 511, 855, 1128
EarI CTCTTC 4 cut(s) 84, 511, 855, 1128
Eco130I CCWWGG 4 cut(s) 1072, 1644, 2338, 2467
Eco24I GRGCYC 2 cut(s) 1803, 1915
Eco32I GATATC 2 cut(s) 1105, 2376
Eco47I GGWCC 2 cut(s) 1205, 1276
Eco57I CTGAAG 4 cut(s) 108, 597, 732, 1391
Eco88I CYCGRG 1 cut(s) 609
Eco91I GGTNACC 1 cut(s) 444
EcoO109I RGGNCCY 4 cut(s) 1205, 1276, 1911, 1912
EcoO65I GGTNACC 1 cut(s) 444
EcoRI GAATTC 2 cut(s) 863, 1331
EcoRII CCWGG 7 cut(s) 36, 216, 834, 1307, 1470, 1518, 2364
EcoRV GATATC 2 cut(s) 1105, 2376
EcoT14I CCWWGG 4 cut(s) 1072, 1644, 2338, 2467
EcoT38I GRGCYC 2 cut(s) 1803, 1915
ErhI CCWWGG 4 cut(s) 1072, 1644, 2338, 2467
Esp3I CGTCTC 1 cut(s) 992
FalI AAGNNNNNCTT 6 cut(s) 290, 322, 1737, 1769, 1884, 1916
FaqI GGGAC 5 cut(s) 691, 944, 968, 1078, 1857
FauI CCCGC 2 cut(s) 370, 1558
FauNDI CATATG 1 cut(s) 1594
FbaI TGATCA 1 cut(s) 1021
Fnu4HI GCNGC 6 cut(s) 603, 732, 1173, 1590, 1706, 2238
FokI GGATG 4 cut(s) 233, 917, 2378, 2447
FriOI GRGCYC 2 cut(s) 1803, 1915
Fsp4HI GCNGC 6 cut(s) 603, 732, 1173, 1590, 1706, 2238
FspBI CTAG 5 cut(s) 147, 155, 237, 1787, 2477
GlaI GCGC 1 cut(s) 291
GluI GCNGC 6 cut(s) 603, 732, 1173, 1590, 1706, 2238
GsaI CCCAGC 2 cut(s) 1062, 2098
GsuI CTGGAG 1 cut(s) 889
HaeIII GGCC 9 cut(s) 406, 644, 1469, 1491, 1701, 1913, 1932, 2597, 2606
HapII CCGG 3 cut(s) 407, 442, 1934
HgaI GACGC 1 cut(s) 1009
HhaI GCGC 1 cut(s) 292
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 1224
HindII GTYRAC 1 cut(s) 1224
HpaI GTTAAC 1 cut(s) 1224
HpaII CCGG 3 cut(s) 407, 442, 1934
HphI GGTGA 4 cut(s) 431, 929, 2177, 2455
Hpy166II GTNNAC 4 cut(s) 1224, 1739, 1766, 2289
Hpy8I GTNNAC 4 cut(s) 1224, 1739, 1766, 2289
HpyAV CCTTC 5 cut(s) 572, 1218, 1288, 1346, 1435
HpyCH4III ACNGT 9 cut(s) 105, 402, 618, 1093, 1399, 1546, 1882, 2023, 2132
HpyCH4IV ACGT 1 cut(s) 1250
HpyF3I CTNAG 5 cut(s) 639, 1259, 1437, 1956, 2300
HpySE526I ACGT 1 cut(s) 1250
HspAI GCGC 1 cut(s) 290
Ksp22I TGATCA 1 cut(s) 1021
KspAI GTTAAC 1 cut(s) 1224
LguI GCTCTTC 1 cut(s) 855
LmnI GCTCC 6 cut(s) 68, 112, 247, 664, 1513, 2389
Lsp1109I GCAGC 6 cut(s) 589, 743, 1184, 1576, 1717, 2224
LweI GCATC 6 cut(s) 211, 328, 816, 1552, 1693, 1721
MaeI CTAG 5 cut(s) 147, 155, 237, 1787, 2477
MaeII ACGT 1 cut(s) 1250
MaeIII GTNAC 5 cut(s) 31, 309, 444, 1546, 2017
MbiI CCGCTC 1 cut(s) 71
MflI RGATCY 3 cut(s) 118, 315, 2473
MhlI GDGCHC 4 cut(s) 616, 1456, 1803, 1915
MlsI TGGCCA 1 cut(s) 2606
MluNI TGGCCA 1 cut(s) 2606
MlyI GAGTC 3 cut(s) 472, 926, 1684
MmeI TCCRAC 1 cut(s) 225
Mox20I TGGCCA 1 cut(s) 2606
MroXI GAANNNNTTC 1 cut(s) 1335
MscI TGGCCA 1 cut(s) 2606
MseI TTAA 8 cut(s) 207, 297, 1008, 1223, 1268, 1580, 2543, 2577
MslI CAYNNNNRTG 1 cut(s) 2222
Msp20I TGGCCA 1 cut(s) 2606
MspA1I CMGCKG 1 cut(s) 551
MspCI CTTAAG 2 cut(s) 206, 1007
MspI CCGG 3 cut(s) 407, 442, 1934
MspR9I CCNGG 8 cut(s) 38, 218, 836, 1309, 1472, 1520, 1934, 2366
Mva1269I GAATGC 2 cut(s) 55, 217
MvaI CCWGG 7 cut(s) 38, 218, 836, 1309, 1472, 1520, 2366
NciI CCSGG 1 cut(s) 1934
NcoI CCATGG 1 cut(s) 1644
NdeI CATATG 1 cut(s) 1594
NheI GCTAGC 1 cut(s) 154
NlaIV GGNNCC 6 cut(s) 840, 1192, 1206, 1278, 1912, 1913
NmuCI GTSAC 1 cut(s) 2017
NspI RCATGY 1 cut(s) 1449
PaeI GCATGC 1 cut(s) 1449
PagI TCATGA 1 cut(s) 1671
PasI CCCWGGG 1 cut(s) 1471
PciSI GCTCTTC 1 cut(s) 855
PctI GAATGC 2 cut(s) 55, 217
PdmI GAANNNNTTC 1 cut(s) 1335
PfeI GAWTC 7 cut(s) 79, 721, 1571, 2065, 2227, 2310, 2582
PfoI TCCNGGA 1 cut(s) 1518
PinAI ACCGGT 1 cut(s) 441
PkrI GCNGC 6 cut(s) 604, 733, 1174, 1591, 1707, 2239
PleI GAGTC 3 cut(s) 472, 926, 1684
PpsI GAGTC 3 cut(s) 472, 926, 1684
PpuMI RGGWCCY 2 cut(s) 1205, 1276
Psp5II RGGWCCY 2 cut(s) 1205, 1276
Psp6I CCWGG 7 cut(s) 36, 216, 834, 1307, 1470, 1518, 2364
PspEI GGTNACC 1 cut(s) 444
PspFI CCCAGC 2 cut(s) 1058, 2094
PspGI CCWGG 7 cut(s) 36, 216, 834, 1307, 1470, 1518, 2364
PspN4I GGNNCC 6 cut(s) 840, 1192, 1206, 1278, 1912, 1913
PspOMI GGGCCC 1 cut(s) 1911
PspPI GGNCC 9 cut(s) 1205, 1276, 1468, 1490, 1699, 1911, 1912, 1931, 2595
PspPPI RGGWCCY 2 cut(s) 1205, 1276
PstI CTGCAG 3 cut(s) 2242, 2400, 2590
PsuI RGATCY 3 cut(s) 118, 315, 2473
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 7 cut(s) 75, 620, 1530, 2003, 2204, 2213, 2288
RsaNI GTAC 7 cut(s) 74, 619, 1529, 2002, 2203, 2212, 2287
RseI CAYNNNNRTG 1 cut(s) 2222
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 8 cut(s) 207, 297, 1008, 1223, 1268, 1580, 2543, 2577
SatI GCNGC 6 cut(s) 603, 732, 1173, 1590, 1706, 2238
Sau96I GGNCC 9 cut(s) 1205, 1276, 1468, 1490, 1699, 1911, 1912, 1931, 2595
ScaI AGTACT 1 cut(s) 2204
SchI GAGTC 3 cut(s) 472, 926, 1684
ScrFI CCNGG 8 cut(s) 38, 218, 836, 1309, 1472, 1520, 1934, 2366
SduI GDGCHC 4 cut(s) 616, 1456, 1803, 1915
SfaNI GCATC 6 cut(s) 211, 328, 816, 1552, 1693, 1721
SfcI CTRYAG 6 cut(s) 60, 330, 552, 2238, 2396, 2586
SfiI GGCCNNNNNGGCC 1 cut(s) 2603
SinI GGWCC 2 cut(s) 1205, 1276
SmiMI CAYNNNNRTG 1 cut(s) 2222
SmlI CTYRAG 6 cut(s) 206, 357, 1007, 1694, 1852, 2102
SmoI CTYRAG 6 cut(s) 206, 357, 1007, 1694, 1852, 2102
SphI GCATGC 1 cut(s) 1449
SsiI CCGC 4 cut(s) 71, 377, 1449, 1565
SspI AATATT 2 cut(s) 436, 1048
SspMI CTAG 5 cut(s) 147, 155, 237, 1787, 2477
StyD4I CCNGG 8 cut(s) 36, 216, 834, 1307, 1470, 1518, 1932, 2364
StyI CCWWGG 4 cut(s) 1072, 1644, 2338, 2467
TaaI ACNGT 9 cut(s) 105, 402, 618, 1093, 1399, 1546, 1882, 2023, 2132
TaiI ACGT 1 cut(s) 1253
TaqI TCGA 6 cut(s) 724, 875, 894, 1131, 1625, 1974
TatI WGTACW 4 cut(s) 618, 1528, 2202, 2286
TfiI GAWTC 7 cut(s) 79, 721, 1571, 2065, 2227, 2310, 2582
Tru1I TTAA 8 cut(s) 207, 297, 1008, 1223, 1268, 1580, 2543, 2577
Tru9I TTAA 8 cut(s) 207, 297, 1008, 1223, 1268, 1580, 2543, 2577
TscAI CASTG 7 cut(s) 407, 621, 1098, 1267, 1549, 2058, 2493
TseFI GTSAC 1 cut(s) 2017
TseI GCWGC 6 cut(s) 602, 731, 1172, 1589, 1705, 2237
Tsp45I GTSAC 1 cut(s) 2017
TspGWI ACGGA 3 cut(s) 91, 2125, 2322
TspRI CASTG 7 cut(s) 407, 621, 1098, 1267, 1549, 2058, 2493
Vha464I CTTAAG 2 cut(s) 206, 1007
VpaK11BI GGWCC 2 cut(s) 1205, 1276
XapI RAATTY 5 cut(s) 631, 863, 1323, 1331, 1990
XbaI TCTAGA 1 cut(s) 146
XceI RCATGY 1 cut(s) 1449
XmnI GAANNNNTTC 1 cut(s) 1335
XspI CTAG 5 cut(s) 147, 155, 237, 1787, 2477
ZrmI AGTACT 1 cut(s) 2204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.