MD14G1134000.v1.1

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002290
Physical Location & Seq
Reverse (-)
21220246 .. 21225772
5527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1134000.v1.1.491

Sequence Viewer

Length: 2622 bp
ATGCTGCTATTATTAGTTACCCAAATTCTTATAAATGCACAAGCAGTTGATGGGAATGTACGCAAGCATGCTGAAGATAGCCTCAGACAGTTTCAAGAACAAAATCTTCCAAGTTTCTTGCTCTCTCTTGCTGGAGAATTGGCCAATGATGAGAAGCCTGTTGAGAGTCGTAAGCTAGCGGGCTTGATCCTCAAGAATGCTTTGGATGCCAAAGAACAACATAGGAAGTTTGAGCTCGGACAAAGATGGTTGTCCTTAGACCCATCCGTGAAGGCCCAGATCAAAGCTTGTTTGTTGAAGACCCTTTCTTCTCCTGCCCACGATGCTCGGTCAACTGCATCTCAAGTTGTTGCAAAGCTTGCCGGGATTGAGTTGCCACTTAAACAGTGGCCTGAACTGATAGAAGCTCTATTATCGAATAATAATCAACTTCCAGCTCATACCAGACAGGCAACTCTTGAAACTCTTGGGTACATATGCGAGGAAGTATCCCCAGATGCTGTAGAGCAAGATCAAGTGAATAAAATGCTTACTGCTGTAGTTCAGGGCATGAACTCTACTGAGACTAACAATGATGTCAGGCTTGCAGCCACAAGAGCTTTGCATGATGCTTTAGGTTTTGCTCAGGCAAATTTCTCAAATGATATGGAGCGTGACTATATAGTGAGAGTTGTATGTGAGGCTACACTTTCCCCTGAAGTGAAGATTCGACAGGCAGCCTTTGAGTGTTTGGTTTCTATATCTTCAACATATTATGAGAAGTTGGCTCCGTATATACAAGATATATTTAGCATCACTGCTAAGGCTGTGAAGGAAGATGAGGAACCTGTTGCACTTCAAGCCATTGAGTTTTGGAGTTCAATATGTGACGAGGAGATTGACATATTAGAAGAATATGGGGGTGATTTTACCGGGGATTCTTCTATTCCATGCTTCTACTTTATCAAGCAAGCACTTCCTGCTCTGATTCCTTTACTATTGGAGACTCTTCTTAAGCAAGAAGAGGATCAAGATCAGGATGAAGTGGCCTGGAATGTTGCAATGGCTGGTGGAACTTGCTTGGGCTTGGTAGCACGAACAGTTGGAGATGATATTGTTCCACTTGTAATGCCATTTATTGAGGAGAATATTACAAAGCCAAATTGGAGGCAGAGGGAGGCAGCCACATATGCATTTGGTTCTATTTTGGAAGGCCCATCCCCTGAAAAGCTCATGCCTCTAGTTAATGTTGCTCTGACCTTCATGCTAAATGCTTTGACGAATGATCCAAATAACCATGTGAAGGACACTACCGGTTGGACTCTTGGAAGAATATTTGAATTTCTTCATGGGTCAACTTTGGAGACGCCCATAATAACCCAAGCGAATTGCCAACAGATTATCACAGTTCTCCTTCAGAGCATGAAAGATGTTCCAAATGTAGCTGAGAAAGCTTGTTGTGCACTCTATTTTCTTGCCCAGGGTTTTGAGGATGCAGGACCTTCTTCCTCTCCTCTGACTCCCTTTTTCCAGGATATCGCCCGAGCCCTTCTAGATGTCACTCACCGAGAAGATGCTGGAGAATCCCGCTTGCGAACTGCAGCTTATGAGGCTCTGAATGAAGTGGTAAGATGTTCCACTGATGAGACTGCTCCATTGGTGATGCAGTTAATTCCTGTCATAATGATGGAACTCCACCAGACACTTGAGGGGCAAAAGCTTTCAGCTGATGAGAGGGAGAAGCAGAATGAATTACAAGGCCTTCTTTGTGGTTGCTTGCAGGTCATCATACAGAAGCTTGGGTCATCTGAACCGACAAAATATGTCTTCATGCAGTATGCTGACCAGATGATGGGTTTGTTCTTGAGAGTGTTTGCTAGTAAAAGTGCCACAGCTCATGAGGAGGCAATGCTTGCCATTGGAGCTCTTGTCTATTCAACAGGCTCAGATTTTGCTAAGTACATGACCGAGTTTTATAAATATTTGGAAATGGGTCTCCAGAATTTTGAGGACTATCAGGTTTGCGCCATCACCGTTGGTGTGGTGGGGGATATATGCAGGGCTTTGGAGGATAAGGTATTGCCTTATTGTGATGGGATTATGACCCAGCTCCTCAAGGATTTGTCCAGCAACCAATTGCACCGATCTGTGAAGCCCCCAATTTTTTCATGCTTTGGTGACCTAGCTTTGGCAATTGGGGAGAATTTCGAGAAGTATTTGATGTATGCTATGCCCATGCTTCAGAGTGCTGCAGAGCTATCTTCCCATACATCAGGTGTCGATGATGATATGGTAGAGTACACAAATACATTGAGAAATGGAATTATGGAGGCGTACTCTGGTATCTTGCAAGGATTTAAGGGCTCCCCAAAGACCCAATTGTTGATGCCGTATGCACCGCATGTTCTTCAGTTCTTGGATAGTTTATACATAGAGAAGGACATGGATGATCTGGTGATAAAGACTGCCATTGGGCTACTTGGAGATCTCGCTGATACGCTTGGTAGTGCTGTTGGCCCGCTAATTATGCAATCTATGTCAGCCAAAGAGTTCCTAAATGAATGTCTCATGTCAGATGATCCTTTGATTAAGGAATCTGCAGAATGGGTCAAGATTGCTATCAGTCGGGCAACTAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

874

Amino Acids

96.61

Weight (kDa)

4.62

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBN_N PF03810 24 - 103 1.9e-13 Importin-beta N-terminal domain
HEAT_EZ PF13513 382 - 438 3.6e-12 HEAT-like repeat
TPR_IMB1 PF25574 460 - 829 3.9e-111 Importin subunit beta-1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 32, 1956
Acc36I ACCTGC 1 cut(s) 1750
AccB7I CCANNNNNTGG 1 cut(s) 1831
AciI CCGC 4 cut(s) 179, 1567, 2378, 2498
AclWI GGATC 4 cut(s) 181, 1014, 1259, 2552
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 5 cut(s) 24, 631, 1319, 1981, 2182
AcuI CTGAAG 5 cut(s) 93, 717, 1379, 2204, 2372
AcyI GRCGYC 1 cut(s) 1346
AfaI GTAC 5 cut(s) 60, 473, 1940, 2279, 2315
AfiI CCNNNNNNNGG 3 cut(s) 1282, 1831, 2167
AflII CTTAAG 1 cut(s) 992
AgeI ACCGGT 1 cut(s) 1292
AgsI TTSAA 8 cut(s) 95, 298, 461, 747, 839, 861, 1319, 1917
AjnI CCWGG 3 cut(s) 1028, 1458, 1509
Alw21I GWGCWC 3 cut(s) 237, 1444, 1906
Alw26I GTCTC 6 cut(s) 557, 977, 1337, 1619, 1979, 2549
Alw44I GTGCAC 1 cut(s) 1440
AlwI GGATC 4 cut(s) 181, 1014, 1259, 2552
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 1521
AoxI GGCC 7 cut(s) 141, 273, 389, 1026, 1192, 1738, 2494
ApaLI GTGCAC 1 cut(s) 1440
ApeKI GCWGC 6 cut(s) 4, 587, 716, 1160, 1580, 2228
ApoI RAATTY 5 cut(s) 24, 631, 1319, 1981, 2182
AsiGI ACCGGT 1 cut(s) 1292
Asp700I GAANNNNTTC 1 cut(s) 1323
AspLEI GCGC 1 cut(s) 2006
AspS9I GGNCC 4 cut(s) 274, 1193, 1478, 2495
AsuC2I CCSGG 2 cut(s) 364, 913
AsuHPI GGTGA 6 cut(s) 914, 1535, 1651, 2002, 2168, 2446
AsuNHI GCTAGC 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 1521
AvaII GGWCC 1 cut(s) 1478
BaeGI GKGCMC 1 cut(s) 1444
BaeI ACNNNNGTAYC 2 cut(s) 2305, 2338
BalI TGGCCA 1 cut(s) 143
BanII GRGCYC 4 cut(s) 237, 1528, 1906, 2345
BbsI GAAGAC 2 cut(s) 305, 1798
Bbv12I GWGCWC 3 cut(s) 237, 1444, 1906
BbvI GCAGC 5 cut(s) 599, 728, 1172, 1592, 2215
BccI CCATC 8 cut(s) 44, 240, 271, 1204, 1660, 1825, 2015, 2066
BceAI ACGGC 1 cut(s) 2353
BciT130I CCWGG 3 cut(s) 1030, 1460, 1511
BciVI GTATCC 1 cut(s) 499
BcnI CCSGG 2 cut(s) 364, 913
BcoDI GTCTC 6 cut(s) 557, 977, 1337, 1619, 1979, 2549
BfaI CTAG 5 cut(s) 176, 1220, 1532, 1857, 2162
BfmI CTRYAG 5 cut(s) 501, 537, 1578, 2229, 2577
BfrI CTTAAG 1 cut(s) 992
BfuAI ACCTGC 1 cut(s) 1750
BfuI GTATCC 1 cut(s) 499
BglII AGATCT 1 cut(s) 2464
BisI GCNGC 6 cut(s) 5, 588, 717, 1161, 1581, 2229
BlsI GCNGC 6 cut(s) 6, 589, 718, 1162, 1582, 2230
Bme1390I CCNGG 5 cut(s) 364, 913, 1030, 1460, 1511
Bme18I GGWCC 1 cut(s) 1478
BmeT110I CYCGRG 1 cut(s) 1521
BmgT120I GGNCC 4 cut(s) 274, 1193, 1478, 2495
BmiI GGNNCC 3 cut(s) 768, 825, 2344
BmrFI CCNGG 5 cut(s) 364, 913, 1030, 1460, 1511
BmtI GCTAGC 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 305, 1798
BplI GAGNNNNNCTC 2 cut(s) 2300, 2332
BpmI CTGGAG 3 cut(s) 153, 1578, 1961
Bpu10I CCTNAGC 2 cut(s) 624, 801
BpuEI CTTGAG 5 cut(s) 176, 327, 1706, 1864, 2078
BpuMI CCSGG 2 cut(s) 364, 913
BsaBI GATNNNNATC 2 cut(s) 1011, 2597
BsaHI GRCGYC 1 cut(s) 1346
BsaI GGTCTC 1 cut(s) 1979
BsaJI CCNNGG 3 cut(s) 912, 1458, 1459
BsaWI WCCGGW 1 cut(s) 1292
BsaXI ACNNNNNCTCC 2 cut(s) 1893, 1923
Bsc4I CCNNNNNNNGG 3 cut(s) 1282, 1831, 2167
Bse118I RCCGGY 1 cut(s) 1292
Bse3DI GCAATG 2 cut(s) 1047, 1893
Bse8I GATNNNNATC 2 cut(s) 1011, 2597
BseBI CCWGG 3 cut(s) 1030, 1460, 1511
BseDI CCNNGG 3 cut(s) 912, 1458, 1459
BseGI GGATG 6 cut(s) 211, 263, 1024, 1196, 1477, 2431
BseJI GATNNNNATC 2 cut(s) 1011, 2597
BseLI CCNNNNNNNGG 3 cut(s) 1282, 1831, 2167
BseMI GCAATG 2 cut(s) 1047, 1893
BseMII CTCAG 5 cut(s) 97, 552, 638, 1416, 1938
BseRI GAGGAG 5 cut(s) 887, 1136, 1482, 1895, 2081
BseSI GKGCMC 1 cut(s) 1444
BseXI GCAGC 5 cut(s) 599, 728, 1172, 1592, 2215
BseYI CCCAGC 1 cut(s) 2085
BshFI GGCC 7 cut(s) 143, 275, 391, 1028, 1194, 1740, 2496
BshTI ACCGGT 1 cut(s) 1292
BsiHKAI GWGCWC 3 cut(s) 237, 1444, 1906
BsiHKCI CYCGRG 1 cut(s) 1521
BsiSI CCGG 3 cut(s) 363, 912, 1293
BslI CCNNNNNNNGG 3 cut(s) 1282, 1831, 2167
BsmAI GTCTC 6 cut(s) 557, 977, 1337, 1619, 1979, 2549
BsmBI CGTCTC 1 cut(s) 1337
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 7 cut(s) 143, 275, 391, 1028, 1194, 1740, 2496
Bso31I GGTCTC 1 cut(s) 1979
BsoBI CYCGRG 1 cut(s) 1521
Bsp1286I GDGCHC 5 cut(s) 237, 1444, 1528, 1906, 2345
BspACI CCGC 4 cut(s) 179, 1567, 2378, 2498
BspANI GGCC 7 cut(s) 143, 275, 391, 1028, 1194, 1740, 2496
BspCNI CTCAG 5 cut(s) 96, 553, 637, 1417, 1937
BspHI TCATGA 1 cut(s) 1876
BspLI GGNNCC 3 cut(s) 768, 825, 2344
BspMAI CTGCAG 3 cut(s) 1582, 2233, 2581
BspMI ACCTGC 1 cut(s) 1750
BspOI GCTAGC 1 cut(s) 179
BspPI GGATC 4 cut(s) 181, 1014, 1259, 2552
BspTI CTTAAG 1 cut(s) 992
BspTNI GGTCTC 1 cut(s) 1979
BsrDI GCAATG 2 cut(s) 1047, 1893
BsrFI RCCGGY 1 cut(s) 1292
BssAI RCCGGY 1 cut(s) 1292
BssECI CCNNGG 3 cut(s) 912, 1458, 1459
BssNI GRCGYC 1 cut(s) 1346
Bst2UI CCWGG 3 cut(s) 1030, 1460, 1511
Bst4CI ACNGT 5 cut(s) 90, 387, 1081, 1387, 2014
Bst6I CTCTTC 2 cut(s) 993, 996
BstACI GRCGYC 1 cut(s) 1346
BstAFI CTTAAG 1 cut(s) 992
BstAPI GCANNNNNTGC 3 cut(s) 359, 959, 1892
BstDEI CTNAG 8 cut(s) 83, 256, 561, 624, 801, 1425, 1924, 1935
BstEII GGTNACC 1 cut(s) 2156
BstF5I GGATG 6 cut(s) 211, 263, 1024, 1196, 1477, 2431
BstHHI GCGC 1 cut(s) 2006
BstMAI GTCTC 6 cut(s) 557, 977, 1337, 1619, 1979, 2549
BstNI CCWGG 3 cut(s) 1030, 1460, 1511
BstNSI RCATGY 2 cut(s) 71, 2384
BstPI GGTNACC 1 cut(s) 2156
BstSCI CCNGG 5 cut(s) 362, 911, 1028, 1458, 1509
BstSFI CTRYAG 5 cut(s) 501, 537, 1578, 2229, 2577
BstSLI GKGCMC 1 cut(s) 1444
BstV1I GCAGC 5 cut(s) 599, 728, 1172, 1592, 2215
BstV2I GAAGAC 2 cut(s) 305, 1798
BstX2I RGATCY 1 cut(s) 2464
BstYI RGATCY 1 cut(s) 2464
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 7 cut(s) 143, 275, 391, 1028, 1194, 1740, 2496
BtsCI GGATG 6 cut(s) 211, 263, 1024, 1196, 1477, 2431
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 3 cut(s) 392, 795, 1617
BveI ACCTGC 1 cut(s) 1750
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 1 cut(s) 1876
CfoI GCGC 1 cut(s) 2006
Cfr10I RCCGGY 1 cut(s) 1292
Cfr13I GGNCC 4 cut(s) 274, 1193, 1478, 2495
CseI GACGC 1 cut(s) 1354
Csp6I GTAC 5 cut(s) 59, 472, 1939, 2278, 2314
CspAI ACCGGT 1 cut(s) 1292
CviQI GTAC 5 cut(s) 59, 472, 1939, 2278, 2314
DdeI CTNAG 8 cut(s) 83, 256, 561, 624, 801, 1425, 1924, 1935
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 2 cut(s) 993, 996
EarI CTCTTC 2 cut(s) 993, 996
Ecl136II GAGCTC 2 cut(s) 235, 1904
Eco147I AGGCCT 1 cut(s) 1740
Eco24I GRGCYC 4 cut(s) 237, 1528, 1906, 2345
Eco31I GGTCTC 1 cut(s) 1979
Eco32I GATATC 1 cut(s) 1516
Eco47I GGWCC 1 cut(s) 1478
Eco53kI GAGCTC 2 cut(s) 235, 1904
Eco57I CTGAAG 5 cut(s) 93, 717, 1379, 2204, 2372
Eco88I CYCGRG 1 cut(s) 1521
Eco91I GGTNACC 1 cut(s) 2156
EcoICRI GAGCTC 2 cut(s) 235, 1904
EcoO109I RGGNCCY 1 cut(s) 1478
EcoO65I GGTNACC 1 cut(s) 2156
EcoRII CCWGG 3 cut(s) 1028, 1458, 1509
EcoRV GATATC 1 cut(s) 1516
EcoT22I ATGCAT 1 cut(s) 1174
EcoT38I GRGCYC 4 cut(s) 237, 1528, 1906, 2345
Esp3I CGTCTC 1 cut(s) 1337
FalI AAGNNNNNCTT 2 cut(s) 1728, 1760
FauI CCCGC 3 cut(s) 172, 1574, 2505
FauNDI CATATG 2 cut(s) 476, 1168
Fnu4HI GCNGC 6 cut(s) 5, 588, 717, 1161, 1581, 2229
FokI GGATG 6 cut(s) 218, 250, 1031, 1183, 1484, 2438
FriOI GRGCYC 4 cut(s) 237, 1528, 1906, 2345
Fsp4HI GCNGC 6 cut(s) 5, 588, 717, 1161, 1581, 2229
FspBI CTAG 5 cut(s) 176, 1220, 1532, 1857, 2162
GlaI GCGC 1 cut(s) 2005
GluI GCNGC 6 cut(s) 5, 588, 717, 1161, 1581, 2229
GsaI CCCAGC 1 cut(s) 2089
GsuI CTGGAG 3 cut(s) 153, 1578, 1961
HaeIII GGCC 7 cut(s) 143, 275, 391, 1028, 1194, 1740, 2496
HapII CCGG 3 cut(s) 363, 912, 1293
HgaI GACGC 1 cut(s) 1354
HhaI GCGC 1 cut(s) 2006
Hin1I GRCGYC 1 cut(s) 1346
Hin6I GCGC 1 cut(s) 2004
HinP1I GCGC 1 cut(s) 2004
HincII GTYRAC 2 cut(s) 333, 1335
HindII GTYRAC 2 cut(s) 333, 1335
HindIII AAGCTT 5 cut(s) 285, 356, 1431, 1697, 1775
HinfI GANTC 9 cut(s) 166, 706, 917, 967, 985, 1300, 1498, 1562, 2573
HpaII CCGG 3 cut(s) 363, 912, 1293
HphI GGTGA 6 cut(s) 914, 1535, 1651, 2002, 2168, 2446
Hpy166II GTNNAC 4 cut(s) 333, 1335, 1442, 2280
Hpy8I GTNNAC 4 cut(s) 333, 1335, 1442, 2280
HpyCH4III ACNGT 5 cut(s) 90, 387, 1081, 1387, 2014
HpyF3I CTNAG 8 cut(s) 83, 256, 561, 624, 801, 1425, 1924, 1935
Hsp92I GRCGYC 1 cut(s) 1346
HspAI GCGC 1 cut(s) 2004
LmnI GCTCC 6 cut(s) 649, 772, 1636, 1901, 2094, 2348
Lsp1109I GCAGC 5 cut(s) 599, 728, 1172, 1592, 2215
MaeI CTAG 5 cut(s) 176, 1220, 1532, 1857, 2162
MaeIII GTNAC 5 cut(s) 16, 653, 866, 1537, 2156
MfeI CAATTG 3 cut(s) 2114, 2172, 2357
MflI RGATCY 1 cut(s) 2464
MhlI GDGCHC 5 cut(s) 237, 1444, 1528, 1906, 2345
MlsI TGGCCA 1 cut(s) 143
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 4 cut(s) 175, 979, 1294, 1492
MmeI TCCRAC 2 cut(s) 1063, 1277
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 1174
MroXI GAANNNNTTC 1 cut(s) 1323
MscI TGGCCA 1 cut(s) 143
MseI TTAA 6 cut(s) 381, 993, 1224, 1649, 2337, 2568
MslI CAYNNNNRTG 1 cut(s) 1664
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 1706
MspCI CTTAAG 1 cut(s) 992
MspI CCGG 3 cut(s) 363, 912, 1293
MspR9I CCNGG 5 cut(s) 364, 913, 1030, 1460, 1511
MunI CAATTG 3 cut(s) 2114, 2172, 2357
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 3 cut(s) 1030, 1460, 1511
NciI CCSGG 2 cut(s) 364, 913
NdeI CATATG 2 cut(s) 476, 1168
NheI GCTAGC 1 cut(s) 175
NlaIV GGNNCC 3 cut(s) 768, 825, 2344
NmuCI GTSAC 4 cut(s) 653, 866, 1537, 2156
NsiI ATGCAT 1 cut(s) 1174
NspI RCATGY 2 cut(s) 71, 2384
PaeI GCATGC 1 cut(s) 71
PagI TCATGA 1 cut(s) 1876
PasI CCCWGGG 1 cut(s) 1459
PceI AGGCCT 1 cut(s) 1740
PctI GAATGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 1323
PfeI GAWTC 5 cut(s) 706, 917, 967, 1562, 2573
PflMI CCANNNNNTGG 1 cut(s) 1831
PfoI TCCNGGA 1 cut(s) 1509
PinAI ACCGGT 1 cut(s) 1292
PkrI GCNGC 6 cut(s) 6, 589, 718, 1162, 1582, 2230
PleI GAGTC 4 cut(s) 174, 979, 1294, 1492
PpsI GAGTC 4 cut(s) 174, 979, 1294, 1492
PpuMI RGGWCCY 1 cut(s) 1478
PsiI TTATAA 2 cut(s) 32, 1956
Psp124BI GAGCTC 2 cut(s) 237, 1906
Psp5II RGGWCCY 1 cut(s) 1478
Psp6I CCWGG 3 cut(s) 1028, 1458, 1509
PspEI GGTNACC 1 cut(s) 2156
PspFI CCCAGC 1 cut(s) 2085
PspGI CCWGG 3 cut(s) 1028, 1458, 1509
PspN4I GGNNCC 3 cut(s) 768, 825, 2344
PspPI GGNCC 4 cut(s) 274, 1193, 1478, 2495
PspPPI RGGWCCY 1 cut(s) 1478
PstI CTGCAG 3 cut(s) 1582, 2233, 2581
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 2464
PvuII CAGCTG 1 cut(s) 1706
RsaI GTAC 5 cut(s) 60, 473, 1940, 2279, 2315
RsaNI GTAC 5 cut(s) 59, 472, 1939, 2278, 2314
RseI CAYNNNNRTG 1 cut(s) 1664
SacI GAGCTC 2 cut(s) 237, 1906
SaqAI TTAA 6 cut(s) 381, 993, 1224, 1649, 2337, 2568
SatI GCNGC 6 cut(s) 5, 588, 717, 1161, 1581, 2229
Sau96I GGNCC 4 cut(s) 274, 1193, 1478, 2495
SchI GAGTC 4 cut(s) 175, 979, 1294, 1492
ScrFI CCNGG 5 cut(s) 364, 913, 1030, 1460, 1511
SduI GDGCHC 5 cut(s) 237, 1444, 1528, 1906, 2345
SfcI CTRYAG 5 cut(s) 501, 537, 1578, 2229, 2577
SinI GGWCC 1 cut(s) 1478
SmiMI CAYNNNNRTG 1 cut(s) 1664
SmlI CTYRAG 6 cut(s) 191, 342, 992, 1685, 1843, 2093
SmoI CTYRAG 6 cut(s) 191, 342, 992, 1685, 1843, 2093
SphI GCATGC 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 1740
SsiI CCGC 4 cut(s) 179, 1567, 2378, 2498
SspI AATATT 3 cut(s) 1129, 1314, 1961
SspMI CTAG 5 cut(s) 176, 1220, 1532, 1857, 2162
SstI GAGCTC 2 cut(s) 237, 1906
StuI AGGCCT 1 cut(s) 1740
StyD4I CCNGG 5 cut(s) 362, 911, 1028, 1458, 1509
TaaI ACNGT 5 cut(s) 90, 387, 1081, 1387, 2014
TaqI TCGA 4 cut(s) 416, 709, 2187, 2259
TaqII GACCGA 2 cut(s) 318, 1961
TatI WGTACW 2 cut(s) 1938, 2277
TfiI GAWTC 5 cut(s) 706, 917, 967, 1562, 2573
Tru1I TTAA 6 cut(s) 381, 993, 1224, 1649, 2337, 2568
Tru9I TTAA 6 cut(s) 381, 993, 1224, 1649, 2337, 2568
TscAI CASTG 3 cut(s) 392, 802, 1624
TseFI GTSAC 4 cut(s) 653, 866, 1537, 2156
TseI GCWGC 6 cut(s) 4, 587, 716, 1160, 1580, 2228
Tsp45I GTSAC 4 cut(s) 653, 866, 1537, 2156
TspGWI ACGGA 2 cut(s) 256, 759
TspRI CASTG 3 cut(s) 392, 802, 1624
Van91I CCANNNNNTGG 1 cut(s) 1831
Vha464I CTTAAG 1 cut(s) 992
VneI GTGCAC 1 cut(s) 1440
VpaK11BI GGWCC 1 cut(s) 1478
XapI RAATTY 5 cut(s) 24, 631, 1319, 1981, 2182
XbaI TCTAGA 1 cut(s) 1531
XceI RCATGY 2 cut(s) 71, 2384
XcmI CCANNNNNNNNNTGG 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 1323
XspI CTAG 5 cut(s) 176, 1220, 1532, 1857, 2162
Zsp2I ATGCAT 1 cut(s) 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.