MD06G1112600.v1.1

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002290
Physical Location & Seq
Reverse (-)
25097088 .. 25100192
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1112600.v1.1.491

Sequence Viewer

Length: 2097 bp
ATGCTTACTGCTGTAGTTCAGGGCATGAACTCTACCGAGACTAACAATGATGTCAGGCTTGCTGCCACTAGAGCTTTGCATAACGCTTTGGGTTTTGCTCAGGCAAATTTCTCCAATGATATGGAGCGTGACTACATAGTAAGAGTTGTATGTGAGGCTACTCTTTCCCCTGAAGTGAAGATTCGACAGGCAGCCTTCGAGTGTTTGGTTTCTATATCTTCAACCTATTATGAGAAGTTGGCTCCGTACATCCAAGATATATTTAGCATCACTGCTAAGGCTGTGAAGGAAGATGAGGAACCTGTTGCACTTCAAGCCATTGAGTTTTGGAGTTCAATCTGCGACGAGGAGATTGACATATTAGAAGAATATGGGGGGGATTTTACTGGGGATTCTTCTATTCCATGCTTTTACTTTATCAAGCAAGCACTTCCTGCCCTAATTCCTTTATTATTGGAGACTCTTCTTAAGCAAGAAGAGGATCAGGATCAGGATGAAGTGGCCTGGAATGTTGCAATGGCTGGTGGAACTTGCTTGGGCTTGGTAGCACGGACAGTTGGAGATGATGTTGTTCCACTTGTAATGCCATTTATTGAGGAGAATATTACAAAGCCAGATTGGAGGCAGAGGGAGGCAGCCACTTACGCATTTGGTTCAATTTTGGAAGGTCCATCCCCTGACAAGCTCATGCCTCTTGTCAATGTTGCTCTGAGTTTCATGCTAAATGCTTTGACGAATGATCCAAATAACCATGTGAAGGACACTACTGGTTGGACTCTTGGAAGAATTTTTGAATTTCTTCATGGGTCAACTTTGGAGACACCCATAATAACCCAAGCGAATTGCCAACAGATTATCACAGTTCTTCTTCAGAGCATGAAGGATGTTCCAAATGTGGCTGAGAAAGCTTGTGGTGCACTCTATTTTCTGGCCCAGGGTTTTGAGGATGCAGAATCTTCCTCCTCCCCTCTGACTTCCTTTTTCCAGGAGATAGCCCAGGCCCTTCTGGATGTTACTCACCGAGAAGATGCTGGCGAATCCCGCTTGCGAACTGCAGCTTATGAGGCTCTGAATGAAGTTGTAAGATGTTCCACTGATGAGACTGCTCCATTGGTGATGCAATTAGTTCCTGTCATAATGATGGAACTCCACCAGACTCTTGAGGCGCAAAAGCTTTCAGCTGATGAGAGGGAGAAGCAGAATGAATTACAAGGCCTTCTTTGCGGTTGCTTGCAGGTCATCATACAGAAGCTAGGGTCATCTGAACCAACAAAATATGTCTTCATGCAGTATGCTGACCAGTTGATGGGTCTGTTCTTGAGAGTATTTGCCAGTAAAAGTGCCACAGCTCATGAGGAGGCCATGCTTGCCATTGGAGCTCTTGTCTACTCAACAGGCTCTGATTTTGCCAAGTACATGACCGAGTTTTATAAATATTTGGAAATGGGTCTCCAGAATTTTGAGGACTATCAGGTTTGCGCCATCACCGTTGGTGTGGTGGGGGATGTATGCAGGGCTTTGGAGGATAAAGTATTACCTTATTGTGATGGGATAATGACCCAGCTCCTCAAGGATTTGTCCAGCAACCAGTTGCACCGATCAGTGAAACCTCCCATATTTTCATGCTTTGGTGACCTAGCTTTGGCAATTGGGGAGAATTTTGAGAAGTATTTGATGTATGCTATGCCCATGCTTCAGAGTGCCGCAGAGCTATCTGCCCATACATCAGGTGTCGATGATGATATGATAGAGTACACAAATACATTGAGAAATGGAATTATGGAGGCGTACTCTGGTATCTTGCAAGGATTTAAGGGCTCCCCAAAGACCCAATTGTTGATGCCGTATGCACCTCATGTTCTCCAGTTCTTGGATAGTTTATACATAGAGAAGGACATGGATGATCTGGTGATAAAGACTGCCATTGGGCTACTTGGAGATCTCGCTGATACGCTGGGTAGTGCTGTCGGTCCGCTAATTCTGCAATCTATGTCAGCCAAAGAGTTCCTAAATGAATGTCTTATGTCAGATGATCCTTCGATAAAGGAATCTGCAGAATGGGTTAAGATCGCTATCAGTCGGGCAACTAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

699

Amino Acids

77.14

Weight (kDa)

4.42

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_EZ PF13513 207 - 263 2.4e-12 HEAT-like repeat
TPR_IMB1 PF25574 285 - 655 8.1e-112 Importin subunit beta-1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1431
Acc36I ACCTGC 1 cut(s) 1225
AccB7I CCANNNNNTGG 2 cut(s) 1306, 1870
AccI GTMKAC 1 cut(s) 1386
AciI CCGC 4 cut(s) 1042, 1224, 1704, 1973
AclWI GGATC 4 cut(s) 489, 495, 734, 2027
AcsI RAATTY 5 cut(s) 106, 786, 794, 1456, 1657
AcuI CTGAAG 3 cut(s) 192, 854, 1679
AfaI GTAC 4 cut(s) 248, 1415, 1754, 1790
AfiI CCNNNNNNNGG 4 cut(s) 757, 1306, 1642, 1870
AflII CTTAAG 1 cut(s) 467
AgsI TTSAA 5 cut(s) 222, 314, 336, 657, 794
AjnI CCWGG 4 cut(s) 503, 933, 984, 996
Alw21I GWGCWC 2 cut(s) 919, 1381
Alw26I GTCTC 5 cut(s) 32, 452, 812, 1094, 1454
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 4 cut(s) 489, 495, 734, 2027
AlwNI CAGNNNCTG 1 cut(s) 1400
AoxI GGCC 5 cut(s) 501, 930, 999, 1213, 1359
ApaLI GTGCAC 1 cut(s) 915
ApeKI GCWGC 4 cut(s) 62, 191, 635, 1055
ApoI RAATTY 5 cut(s) 106, 786, 794, 1456, 1657
Asp700I GAANNNNTTC 1 cut(s) 798
AspLEI GCGC 2 cut(s) 1168, 1481
AspS9I GGNCC 4 cut(s) 668, 931, 1000, 1970
AsuHPI GGTGA 5 cut(s) 1010, 1126, 1477, 1643, 1921
AvaII GGWCC 2 cut(s) 668, 1970
BaeGI GKGCMC 1 cut(s) 919
BaeI ACNNNNGTAYC 2 cut(s) 1780, 1813
BanII GRGCYC 2 cut(s) 1381, 1820
BbsI GAAGAC 1 cut(s) 1273
Bbv12I GWGCWC 2 cut(s) 919, 1381
BbvI GCAGC 4 cut(s) 49, 203, 647, 1067
BccI CCATC 5 cut(s) 679, 1135, 1300, 1490, 1541
BceAI ACGGC 1 cut(s) 1828
BciT130I CCWGG 4 cut(s) 505, 935, 986, 998
BcoDI GTCTC 5 cut(s) 32, 452, 812, 1094, 1454
BfaI CTAG 3 cut(s) 69, 1253, 1637
BfmI CTRYAG 3 cut(s) 12, 1053, 2052
BfrI CTTAAG 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 1225
BglII AGATCT 1 cut(s) 1939
BisI GCNGC 5 cut(s) 63, 192, 636, 1056, 1704
BlsI GCNGC 5 cut(s) 64, 193, 637, 1057, 1705
Bme1390I CCNGG 4 cut(s) 505, 935, 986, 998
Bme18I GGWCC 2 cut(s) 668, 1970
BmgT120I GGNCC 4 cut(s) 668, 931, 1000, 1970
BmiI GGNNCC 3 cut(s) 243, 300, 1819
BmrFI CCNGG 4 cut(s) 505, 935, 986, 998
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 5 cut(s) 276, 937, 1018, 1107, 1830
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 1273
BplI GAGNNNNNCTC 2 cut(s) 1775, 1807
BpmI CTGGAG 2 cut(s) 1436, 1847
Bpu10I CCTNAGC 2 cut(s) 99, 276
BpuEI CTTGAG 3 cut(s) 1181, 1339, 1553
BsaBI GATNNNNATC 2 cut(s) 486, 2072
BsaI GGTCTC 1 cut(s) 1454
BsaJI CCNNGG 3 cut(s) 933, 934, 996
BsaXI ACNNNNNCTCC 4 cut(s) 552, 582, 1368, 1398
Bsc4I CCNNNNNNNGG 4 cut(s) 757, 1306, 1642, 1870
Bse1I ACTGG 6 cut(s) 391, 772, 1300, 1332, 1588, 1864
Bse3DI GCAATG 1 cut(s) 522
Bse8I GATNNNNATC 2 cut(s) 486, 2072
BseBI CCWGG 4 cut(s) 505, 935, 986, 998
BseDI CCNNGG 3 cut(s) 933, 934, 996
BseGI GGATG 8 cut(s) 249, 499, 671, 889, 952, 1015, 1510, 1906
BseJI GATNNNNATC 2 cut(s) 486, 2072
BseLI CCNNNNNNNGG 4 cut(s) 757, 1306, 1642, 1870
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 3 cut(s) 113, 701, 891
BseNI ACTGG 6 cut(s) 391, 772, 1300, 1332, 1588, 1864
BseRI GAGGAG 5 cut(s) 362, 611, 952, 1370, 1556
BseSI GKGCMC 1 cut(s) 919
BseXI GCAGC 4 cut(s) 49, 203, 647, 1067
BseYI CCCAGC 2 cut(s) 1560, 1954
BshFI GGCC 5 cut(s) 503, 932, 1001, 1215, 1361
BsiHKAI GWGCWC 2 cut(s) 919, 1381
BslI CCNNNNNNNGG 4 cut(s) 757, 1306, 1642, 1870
BsmAI GTCTC 5 cut(s) 32, 452, 812, 1094, 1454
BsnI GGCC 5 cut(s) 503, 932, 1001, 1215, 1361
Bso31I GGTCTC 1 cut(s) 1454
Bsp1286I GDGCHC 3 cut(s) 919, 1381, 1820
Bsp143I GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
BspACI CCGC 4 cut(s) 1042, 1224, 1704, 1973
BspANI GGCC 5 cut(s) 503, 932, 1001, 1215, 1361
BspCNI CTCAG 3 cut(s) 112, 702, 892
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 3 cut(s) 243, 300, 1819
BspMAI CTGCAG 2 cut(s) 1057, 2056
BspMI ACCTGC 1 cut(s) 1225
BspPI GGATC 4 cut(s) 489, 495, 734, 2027
BspTI CTTAAG 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 1454
BsrDI GCAATG 1 cut(s) 522
BsrI ACTGG 6 cut(s) 391, 772, 1300, 1332, 1588, 1864
BssECI CCNNGG 3 cut(s) 933, 934, 996
BssMI GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
Bst2UI CCWGG 4 cut(s) 505, 935, 986, 998
Bst4CI ACNGT 3 cut(s) 556, 862, 1489
Bst6I CTCTTC 2 cut(s) 468, 471
BstAFI CTTAAG 1 cut(s) 467
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 6 cut(s) 60, 426, 1033, 1046, 1232, 1368
BstDEI CTNAG 4 cut(s) 99, 276, 710, 900
BstEII GGTNACC 1 cut(s) 1631
BstF5I GGATG 8 cut(s) 249, 499, 671, 889, 952, 1015, 1510, 1906
BstHHI GCGC 2 cut(s) 1168, 1481
BstKTI GATC 8 cut(s) 484, 490, 742, 1601, 1906, 1942, 2035, 2070
BstMAI GTCTC 5 cut(s) 32, 452, 812, 1094, 1454
BstMBI GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
BstNI CCWGG 4 cut(s) 505, 935, 986, 998
BstPI GGTNACC 1 cut(s) 1631
BstSCI CCNGG 4 cut(s) 503, 933, 984, 996
BstSFI CTRYAG 3 cut(s) 12, 1053, 2052
BstSLI GKGCMC 1 cut(s) 919
BstV1I GCAGC 4 cut(s) 49, 203, 647, 1067
BstV2I GAAGAC 1 cut(s) 1273
BstX2I RGATCY 1 cut(s) 1939
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 1 cut(s) 1939
BsuRI GGCC 5 cut(s) 503, 932, 1001, 1215, 1361
BtsCI GGATG 8 cut(s) 249, 499, 671, 889, 952, 1015, 1510, 1906
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 3 cut(s) 270, 1092, 1608
BveI ACCTGC 1 cut(s) 1225
Cac8I GCNNGC 6 cut(s) 60, 426, 1033, 1046, 1232, 1368
CaiI CAGNNNCTG 1 cut(s) 1400
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 2 cut(s) 1168, 1481
Cfr13I GGNCC 4 cut(s) 668, 931, 1000, 1970
CpoI CGGWCCG 1 cut(s) 1970
Csp6I GTAC 4 cut(s) 247, 1414, 1753, 1789
CspI CGGWCCG 1 cut(s) 1970
CviQI GTAC 4 cut(s) 247, 1414, 1753, 1789
DdeI CTNAG 4 cut(s) 99, 276, 710, 900
DpnI GATC 8 cut(s) 483, 489, 741, 1600, 1905, 1941, 2034, 2069
DpnII GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
Eam1104I CTCTTC 2 cut(s) 468, 471
EarI CTCTTC 2 cut(s) 468, 471
Ecl136II GAGCTC 1 cut(s) 1379
Eco147I AGGCCT 1 cut(s) 1215
Eco24I GRGCYC 2 cut(s) 1381, 1820
Eco31I GGTCTC 1 cut(s) 1454
Eco47I GGWCC 2 cut(s) 668, 1970
Eco53kI GAGCTC 1 cut(s) 1379
Eco57I CTGAAG 3 cut(s) 192, 854, 1679
Eco91I GGTNACC 1 cut(s) 1631
EcoICRI GAGCTC 1 cut(s) 1379
EcoO109I RGGNCCY 1 cut(s) 1000
EcoO65I GGTNACC 1 cut(s) 1631
EcoRII CCWGG 4 cut(s) 503, 933, 984, 996
EcoT38I GRGCYC 2 cut(s) 1381, 1820
FalI AAGNNNNNCTT 2 cut(s) 1203, 1235
FauI CCCGC 1 cut(s) 1049
FblI GTMKAC 1 cut(s) 1386
Fnu4HI GCNGC 5 cut(s) 63, 192, 636, 1056, 1704
FokI GGATG 8 cut(s) 236, 506, 658, 896, 959, 1022, 1517, 1913
FriOI GRGCYC 2 cut(s) 1381, 1820
Fsp4HI GCNGC 5 cut(s) 63, 192, 636, 1056, 1704
FspBI CTAG 3 cut(s) 69, 1253, 1637
GlaI GCGC 2 cut(s) 1167, 1480
GluI GCNGC 5 cut(s) 63, 192, 636, 1056, 1704
GsaI CCCAGC 2 cut(s) 1564, 1958
GsuI CTGGAG 2 cut(s) 1436, 1847
HaeIII GGCC 5 cut(s) 503, 932, 1001, 1215, 1361
HhaI GCGC 2 cut(s) 1168, 1481
Hin6I GCGC 2 cut(s) 1166, 1479
HinP1I GCGC 2 cut(s) 1166, 1479
HincII GTYRAC 1 cut(s) 810
HindII GTYRAC 1 cut(s) 810
HindIII AAGCTT 2 cut(s) 906, 1172
HinfI GANTC 8 cut(s) 181, 392, 460, 775, 953, 1037, 1156, 2048
HphI GGTGA 5 cut(s) 1010, 1126, 1477, 1643, 1921
Hpy166II GTNNAC 4 cut(s) 810, 917, 1387, 1755
Hpy188I TCNGA 8 cut(s) 711, 873, 972, 1071, 1264, 1402, 1698, 2029
Hpy188III TCNNGA 7 cut(s) 485, 491, 1007, 1160, 1318, 1352, 1453
Hpy8I GTNNAC 4 cut(s) 810, 917, 1387, 1755
Hpy99I CGWCG 1 cut(s) 347
HpyAV CCTTC 9 cut(s) 205, 280, 659, 751, 874, 1013, 1226, 1885, 2046
HpyCH4III ACNGT 3 cut(s) 556, 862, 1489
HpyF3I CTNAG 4 cut(s) 99, 276, 710, 900
HspAI GCGC 2 cut(s) 1166, 1479
Kzo9I GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
LmnI GCTCC 6 cut(s) 124, 247, 1111, 1376, 1569, 1823
Lsp1109I GCAGC 4 cut(s) 49, 203, 647, 1067
LweI GCATC 5 cut(s) 276, 937, 1018, 1107, 1830
MaeI CTAG 3 cut(s) 69, 1253, 1637
MaeIII GTNAC 3 cut(s) 128, 1012, 1631
MalI GATC 8 cut(s) 483, 489, 741, 1600, 1905, 1941, 2034, 2069
MboI GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
MfeI CAATTG 2 cut(s) 1647, 1832
MflI RGATCY 1 cut(s) 1939
MhlI GDGCHC 3 cut(s) 919, 1381, 1820
MlyI GAGTC 3 cut(s) 454, 769, 1150
MmeI TCCRAC 2 cut(s) 538, 752
MroXI GAANNNNTTC 1 cut(s) 798
MseI TTAA 3 cut(s) 468, 1812, 2064
MslI CAYNNNNRTG 1 cut(s) 1139
MspA1I CMGCKG 1 cut(s) 1181
MspCI CTTAAG 1 cut(s) 467
MspR9I CCNGG 4 cut(s) 505, 935, 986, 998
MunI CAATTG 2 cut(s) 1647, 1832
MvaI CCWGG 4 cut(s) 505, 935, 986, 998
NdeII GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
NlaIV GGNNCC 3 cut(s) 243, 300, 1819
NmuCI GTSAC 2 cut(s) 128, 1631
PagI TCATGA 1 cut(s) 1351
PasI CCCWGGG 1 cut(s) 934
PceI AGGCCT 1 cut(s) 1215
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 5 cut(s) 181, 392, 953, 1037, 2048
PflMI CCANNNNNTGG 2 cut(s) 1306, 1870
PfoI TCCNGGA 1 cut(s) 984
PkrI GCNGC 5 cut(s) 64, 193, 637, 1057, 1705
PleI GAGTC 3 cut(s) 454, 769, 1150
PpsI GAGTC 3 cut(s) 454, 769, 1150
PsiI TTATAA 1 cut(s) 1431
Psp124BI GAGCTC 1 cut(s) 1381
Psp6I CCWGG 4 cut(s) 503, 933, 984, 996
PspEI GGTNACC 1 cut(s) 1631
PspFI CCCAGC 2 cut(s) 1560, 1954
PspGI CCWGG 4 cut(s) 503, 933, 984, 996
PspN4I GGNNCC 3 cut(s) 243, 300, 1819
PspPI GGNCC 4 cut(s) 668, 931, 1000, 1970
PstI CTGCAG 2 cut(s) 1057, 2056
PstNI CAGNNNCTG 1 cut(s) 1400
PsuI RGATCY 1 cut(s) 1939
PvuII CAGCTG 1 cut(s) 1181
RsaI GTAC 4 cut(s) 248, 1415, 1754, 1790
RsaNI GTAC 4 cut(s) 247, 1414, 1753, 1789
RseI CAYNNNNRTG 1 cut(s) 1139
Rsr2I CGGWCCG 1 cut(s) 1970
RsrII CGGWCCG 1 cut(s) 1970
SacI GAGCTC 1 cut(s) 1381
SaqAI TTAA 3 cut(s) 468, 1812, 2064
SatI GCNGC 5 cut(s) 63, 192, 636, 1056, 1704
Sau3AI GATC 8 cut(s) 481, 487, 739, 1598, 1903, 1939, 2032, 2067
Sau96I GGNCC 4 cut(s) 668, 931, 1000, 1970
SchI GAGTC 3 cut(s) 454, 769, 1150
ScrFI CCNGG 4 cut(s) 505, 935, 986, 998
SduI GDGCHC 3 cut(s) 919, 1381, 1820
SfaNI GCATC 5 cut(s) 276, 937, 1018, 1107, 1830
SfcI CTRYAG 3 cut(s) 12, 1053, 2052
SinI GGWCC 2 cut(s) 668, 1970
SmiMI CAYNNNNRTG 1 cut(s) 1139
SmlI CTYRAG 4 cut(s) 467, 1160, 1318, 1568
SmoI CTYRAG 4 cut(s) 467, 1160, 1318, 1568
SseBI AGGCCT 1 cut(s) 1215
SsiI CCGC 4 cut(s) 1042, 1224, 1704, 1973
SspI AATATT 2 cut(s) 604, 1436
SspMI CTAG 3 cut(s) 69, 1253, 1637
SstI GAGCTC 1 cut(s) 1381
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 4 cut(s) 503, 933, 984, 996
TaaI ACNGT 3 cut(s) 556, 862, 1489
TaqI TCGA 4 cut(s) 184, 198, 1734, 2039
TaqII GACCGA 2 cut(s) 1436, 1958
TatI WGTACW 2 cut(s) 1413, 1752
TauI GCSGC 1 cut(s) 1706
TfiI GAWTC 5 cut(s) 181, 392, 953, 1037, 2048
Tru1I TTAA 3 cut(s) 468, 1812, 2064
Tru9I TTAA 3 cut(s) 468, 1812, 2064
TscAI CASTG 3 cut(s) 277, 1099, 1608
TseFI GTSAC 2 cut(s) 128, 1631
TseI GCWGC 4 cut(s) 62, 191, 635, 1055
Tsp45I GTSAC 2 cut(s) 128, 1631
TspGWI ACGGA 2 cut(s) 234, 565
TspRI CASTG 3 cut(s) 277, 1099, 1608
Van91I CCANNNNNTGG 2 cut(s) 1306, 1870
Vha464I CTTAAG 1 cut(s) 467
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 668, 1970
XapI RAATTY 5 cut(s) 106, 786, 794, 1456, 1657
XmiI GTMKAC 1 cut(s) 1386
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 3 cut(s) 69, 1253, 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.