RLG00000004852

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002290
Physical Location & Seq
Forward (+)
63075755 .. 63080373
4619 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004852

Sequence Viewer

Length: 2646 bp
ATGTCCACTCATCAACTCGGGATCCCAATGGCAATGGAAATTACCCAGTTTCTTTTAAATGCCCAAGCAGTGGATGGAAATGTACGCAAGCATGCTGAAGAGAACATAAGACAGTTTCAAGAGCAAAATCTTCCGAGTTTCCTGTTCTCTCTTGCTGCAGAATTAGCTAATGATGACAAGCCTGTTGAGAGTCGAAAGTTAGCAGGTCTAATTCTAAAGAATGCATTGGATGCCAAAGAACAGCATAGAAAGTTTGAACTTGCACAAAGATGGATTGCTCTGGACCCAAATGCAAAGACCCAAATTAGAGCGTTCCTGTTAAAGACTTTATCATCTCCTGCCCATGATGCTCGATCAACTGCATCTCAAGTCATTGCAAAGCTTGCAGGCATTGAGTTGCCACTCAAACATTGGCCTGAACTGATAGAAGCTCTGCTGTCAAATAATAATCAGCTTCCGGCTCATACGAGACAGGCAACTCTGGAAACTCTTGGATACATATGTGAGGAGGTCTCCCCAGAGGTGATAGAGCAAGATCATGTCAACAAAATACTTACAGCTGTTGTTCAGGGCATGAACTCTTCCGAGAGTAATAATGATGTCAGGCTTGCTGCCACACGAGCTCTTTATAATGCTTTGGGTTTTGCTCAGGCGAACTTCTCCAATGATACGGAACGTGATTACATAATGAGAGTTGTATGTGAGGCTACTCTTTCCCCTGAGGTAAAGATTCGACAAGCAGCATTTGAATGTTTGGTTTCTATATCGTCGACATACTATGAGAAATTGTCTCCATACATCCAAGATATTTTCACCATAACTGCAAAGGCTGTCAAGGAAGATGAAGAACCTGTTGCGCTTCAAGCTATTGAGTTTTGGAGTTCAATTTGTGATGAGGAGATTGACATTCTAGAAGAATATGGTGGTGATTTCACTGGGGAATCTGTTATTCCTTGCTTTTATTTTATTAAGCAAGCACTTCCTGCTCTCATCCCCTTGTTATTGGAGACACTTCTTAAGCAGGAAGAGGATCAAGATCAGGATGAAGGGGCCTGGAATATTGCCATGGCTGGTGGCACTTGCTTGGGCTTGGTAGCACGGACAGTTGGTGATGATGTTGTGCCGCTTGTAATGCCATTTATTGAGGAGAATATTTCAAAACCAGATTGGAGGCAGAGGGAGGCAGCCACCTATGCTTTTGGTTCAGTTTTGGAAGGCCCATCCCCTGAGAAGCTGATGACTCTTGTTAATCATGCTTTGAATTTTATGCTAAATGCCCTGATGAAAGATCCAAACAACCATGTGAAGGACACTACTGGTTGGACCCTTGGAAGGATATTTGAGTTTCTACATGGGTCAACTTATGAGACACCCATAATTACCCAAGCAAATTGTCAACAGATTATCACAGTTCTTCTTCAGAGCATGAAAGATGTACCAAATGTTGCTGAGAAAGCTTGTGGTGCTTTATATTTCTTGGCGCAGGGTTTCGAGGATTCAGGGTCTTCATCCACCCAGCTGACTCCCTTTTTCCAGGATATCATCGGGGCACTTCTTGACGTGACTCATCGAGAAGATGCTGGAGAATCCCGCCTTCGAACTGCAGCCTATGAGAGTCTGAATGAAGTTGTAAGGTGCTGCACTGATGAGACTGCTCCAATGGTGATGCAATTAGTTCCTATTATTATGAATGAACTCCACCAGACTCTTGAGGCACAGAGGCTTTCATCTGATGAAAGAGACAAGCAGAATGAACTACAAGGCCTTCTCTGTGGTTGCTTGCAGGTCATCATACAGAAGCTAGGGTCATCTGAACCAACTAAATATATCTTCATGCAGTATGCTGACCAGATGATGCAACTTTTCTTGAGAGTATTTTCTTGTAAAAGTGCCACAGCGCATGAGGAGGCTATGCTTGCCATTGGAGCTCTTGCCTATTCAACTGGCTCTGATTTTGCGAAGTACATGTCTGAATTTTACAAATATTTAGAAATGGGCCTCCAAAACTTTGAGGACTATCAGGTTTGTGCTATAACAGTTGGTGTGGTGGGGGATATATCTAGGGCTTTGGAAGATAAAATATTGCCTTATTGTGATGGGATAATGACCCAGCTCCTCAAGAATTTGTCAAGCAACCAGTTGCACCGATCTGTGAAGCCTCCAATATTTTCATGCTTTGGTGACCTGGCTTTGGCCATTGGGGAGAATTTCGACAAGTATTTGATGTATGCCATGCCCATGCTTCAGAGTGCGGCAGAACTTTCAGTCCAGACATCAGGTGCTGATGATGATATAATAGAGTACACAAATACTTTGAGGAATGGAATTTTGGAGGCATACTCTGGTATCTTACAAGGATTTAAGGGCTCCGCAAAGAGCCAATTATTGATGCCTCATGCACCTCATATTCTCCAGTTCTTGGATAGTTTATACATGGCTAAGGACATGGATGATGTGGTCATGAAGACTGCCATTGGGGTACTTGGAGATCTAGCTGATACATTGGGTGGTAATGCAGCTCCGTTAATTCAGCAATGTGTATCAAGCAGAGGCTTCCTAAATGAATGTCTCCAGTCAGAGGATAATTTGGTCAAGGAAGCTGGTGAATGGGCGCAGTTGGCCATTCGTCGGGCAATATCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

882

Amino Acids

97.77

Weight (kDa)

4.69

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBN_N PF03810 32 - 111 1.2e-12 Importin-beta N-terminal domain
HEAT_EZ PF13513 390 - 446 9e-10 HEAT-like repeat
TPR_IMB1 PF25574 468 - 839 5.8e-111 Importin subunit beta-1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
Acc36I ACCTGC 2 cut(s) 194, 1774
AccB7I CCANNNNNTGG 2 cut(s) 70, 2419
AccI GTMKAC 1 cut(s) 770
AciI CCGC 4 cut(s) 1124, 1591, 2252, 2370
AclWI GGATC 4 cut(s) 16, 29, 1038, 1283
AcoI YGGCCR 2 cut(s) 2193, 2619
AcsI RAATTY 5 cut(s) 1261, 1973, 2122, 2206, 2325
AcuI CTGAAG 3 cut(s) 117, 1403, 2228
AfaI GTAC 5 cut(s) 84, 1437, 1964, 2303, 2481
AfiI CCNNNNNNNGG 7 cut(s) 70, 1306, 1332, 2191, 2419, 2578, 2628
AflII CTTAAG 1 cut(s) 1016
AflIII ACRYGT 1 cut(s) 1965
AgsI TTSAA 8 cut(s) 119, 257, 749, 863, 885, 1158, 1261, 1941
AjiI CACGTC 1 cut(s) 1561
AjnI CCWGG 3 cut(s) 1052, 1533, 2184
AjuI GAANNNNNNNTTGG 2 cut(s) 2312, 2344
AloI GAACNNNNNNTCC 2 cut(s) 2250, 2282
Alw21I GWGCWC 2 cut(s) 625, 1930
Alw26I GTCTC 8 cut(s) 463, 517, 795, 1001, 1361, 1643, 1734, 2573
AlwI GGATC 4 cut(s) 16, 29, 1038, 1283
AlwNI CAGNNNCTG 1 cut(s) 2282
Ama87I CYCGRG 1 cut(s) 17
AoxI GGCC 7 cut(s) 413, 1050, 1216, 1762, 1996, 2193, 2619
ApeKI GCWGC 7 cut(s) 155, 611, 740, 1184, 1604, 1638, 2516
ApoI RAATTY 5 cut(s) 1261, 1973, 2122, 2206, 2325
ArsI GACNNNNNNTTYG 2 cut(s) 1952, 1984
AspLEI GCGC 4 cut(s) 859, 1483, 1900, 2614
AspS9I GGNCC 5 cut(s) 283, 1050, 1217, 1323, 1996
AsuHPI GGTGA 7 cut(s) 535, 805, 938, 1121, 1675, 2192, 2615
AsuII TTCGAA 1 cut(s) 1597
AvaI CYCGRG 1 cut(s) 17
AvaII GGWCC 2 cut(s) 283, 1323
AxyI CCTNAGG 1 cut(s) 720
BaeGI GKGCMC 1 cut(s) 1552
BaeI ACNNNNGTAYC 4 cut(s) 487, 520, 2329, 2362
BalI TGGCCA 2 cut(s) 2195, 2621
BamHI GGATCC 1 cut(s) 21
BanII GRGCYC 3 cut(s) 625, 1930, 2369
BauI CACGAG 1 cut(s) 618
BbsI GAAGAC 2 cut(s) 1497, 2471
Bbv12I GWGCWC 2 cut(s) 625, 1930
BbvI GCAGC 7 cut(s) 142, 598, 752, 1196, 1616, 1625, 2528
BccI CCATC 4 cut(s) 68, 264, 1228, 2090
BciT130I CCWGG 3 cut(s) 1054, 1535, 2186
BciVI GTATCC 1 cut(s) 488
BcoDI GTCTC 8 cut(s) 463, 517, 795, 1001, 1361, 1643, 1734, 2573
BfaI CTAG 4 cut(s) 911, 1802, 2061, 2492
BfmI CTRYAG 2 cut(s) 156, 1602
BfrI CTTAAG 1 cut(s) 1016
BfuAI ACCTGC 2 cut(s) 194, 1774
BfuI GTATCC 1 cut(s) 488
BglII AGATCT 1 cut(s) 2488
BisI GCNGC 9 cut(s) 156, 612, 741, 1124, 1185, 1605, 1639, 2253, 2517
BlsI GCNGC 9 cut(s) 157, 613, 742, 1125, 1186, 1606, 1640, 2254, 2518
Bme1390I CCNGG 3 cut(s) 1054, 1535, 2186
Bme18I GGWCC 2 cut(s) 283, 1323
BmeT110I CYCGRG 1 cut(s) 17
BmgBI CACGTC 1 cut(s) 1561
BmgT120I GGNCC 5 cut(s) 283, 1050, 1217, 1323, 1996
BmiI GGNNCC 5 cut(s) 23, 285, 1051, 1325, 2368
BmrFI CCNGG 3 cut(s) 1054, 1535, 2186
BmrI ACTGGG 2 cut(s) 40, 945
BmsI GCATC 7 cut(s) 220, 337, 371, 1567, 1656, 1845, 2379
BmuI ACTGGG 2 cut(s) 40, 945
BpiI GAAGAC 2 cut(s) 1497, 2471
BplI GAGNNNNNCTC 4 cut(s) 497, 529, 2324, 2356
BpmI CTGGAG 3 cut(s) 1602, 2396, 2555
Bpu10I CCTNAGC 2 cut(s) 648, 2439
Bpu14I TTCGAA 1 cut(s) 1597
BpuEI CTTGAG 4 cut(s) 351, 1730, 1888, 2102
BsaBI GATNNNNATC 1 cut(s) 1035
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 2 cut(s) 1065, 1327
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 1306, 1332, 2191, 2419, 2578, 2628
Bse1I ACTGG 7 cut(s) 46, 940, 1321, 1948, 2137, 2413, 2572
Bse21I CCTNAGG 1 cut(s) 720
Bse3DI GCAATG 3 cut(s) 39, 372, 2540
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 3 cut(s) 1054, 1535, 2186
BseDI CCNNGG 2 cut(s) 1065, 1327
BseGI GGATG 8 cut(s) 79, 235, 798, 990, 1048, 1220, 1508, 2455
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 7 cut(s) 70, 1306, 1332, 2191, 2419, 2578, 2628
BseMI GCAATG 3 cut(s) 39, 372, 2540
BseMII CTCAG 4 cut(s) 662, 711, 1218, 1440
BseNI ACTGG 7 cut(s) 46, 940, 1321, 1948, 2137, 2413, 2572
BseRI GAGGAG 5 cut(s) 521, 911, 1160, 1919, 2105
BseSI GKGCMC 1 cut(s) 1552
BseXI GCAGC 7 cut(s) 142, 598, 752, 1196, 1616, 1625, 2528
BseYI CCCAGC 2 cut(s) 1515, 2109
BsgI GTGCAG 1 cut(s) 1624
BshFI GGCC 7 cut(s) 415, 1052, 1218, 1764, 1998, 2195, 2621
BsiHKAI GWGCWC 2 cut(s) 625, 1930
BsiHKCI CYCGRG 1 cut(s) 17
BsiSI CCGG 1 cut(s) 458
BslI CCNNNNNNNGG 7 cut(s) 70, 1306, 1332, 2191, 2419, 2578, 2628
BsmAI GTCTC 8 cut(s) 463, 517, 795, 1001, 1361, 1643, 1734, 2573
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 7 cut(s) 415, 1052, 1218, 1764, 1998, 2195, 2621
Bso31I GGTCTC 1 cut(s) 517
BsoBI CYCGRG 1 cut(s) 17
Bsp119I TTCGAA 1 cut(s) 1597
Bsp1286I GDGCHC 4 cut(s) 625, 1552, 1930, 2369
Bsp143I GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
Bsp19I CCATGG 1 cut(s) 1065
BspACI CCGC 4 cut(s) 1124, 1591, 2252, 2370
BspANI GGCC 7 cut(s) 415, 1052, 1218, 1764, 1998, 2195, 2621
BspCNI CTCAG 4 cut(s) 661, 712, 1219, 1441
BspHI TCATGA 1 cut(s) 2460
BspLI GGNNCC 5 cut(s) 23, 285, 1051, 1325, 2368
BspMAI CTGCAG 2 cut(s) 160, 1606
BspMI ACCTGC 2 cut(s) 194, 1774
BspPI GGATC 4 cut(s) 16, 29, 1038, 1283
BspT104I TTCGAA 1 cut(s) 1597
BspTI CTTAAG 1 cut(s) 1016
BspTNI GGTCTC 1 cut(s) 517
BsrDI GCAATG 3 cut(s) 39, 372, 2540
BsrI ACTGG 7 cut(s) 46, 940, 1321, 1948, 2137, 2413, 2572
BssECI CCNNGG 2 cut(s) 1065, 1327
BssMI GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 2 cut(s) 1065, 1327
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 3 cut(s) 1054, 1535, 2186
Bst4CI ACNGT 4 cut(s) 114, 1105, 1411, 2038
Bst6I CTCTTC 3 cut(s) 93, 586, 1020
BstAFI CTTAAG 1 cut(s) 1016
BstAPI GCANNNNNTGC 3 cut(s) 230, 383, 983
BstBI TTCGAA 1 cut(s) 1597
BstC8I GCNNGC 8 cut(s) 89, 93, 384, 388, 609, 975, 1781, 1917
BstDEI CTNAG 5 cut(s) 648, 720, 1227, 1449, 2439
BstDSI CCRYGG 1 cut(s) 1065
BstEII GGTNACC 1 cut(s) 2180
BstF5I GGATG 8 cut(s) 79, 235, 798, 990, 1048, 1220, 1508, 2455
BstHHI GCGC 4 cut(s) 859, 1483, 1900, 2614
BstKTI GATC 8 cut(s) 24, 356, 538, 1033, 1039, 1291, 2150, 2491
BstMAI GTCTC 8 cut(s) 463, 517, 795, 1001, 1361, 1643, 1734, 2573
BstMBI GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
BstNI CCWGG 3 cut(s) 1054, 1535, 2186
BstNSI RCATGY 2 cut(s) 95, 1969
BstPI GGTNACC 1 cut(s) 2180
BstSCI CCNGG 3 cut(s) 1052, 1533, 2184
BstSFI CTRYAG 2 cut(s) 156, 1602
BstSLI GKGCMC 1 cut(s) 1552
BstV1I GCAGC 7 cut(s) 142, 598, 752, 1196, 1616, 1625, 2528
BstV2I GAAGAC 2 cut(s) 1497, 2471
BstX2I RGATCY 3 cut(s) 21, 1288, 2488
BstYI RGATCY 3 cut(s) 21, 1288, 2488
Bsu36I CCTNAGG 1 cut(s) 720
BsuI GTATCC 1 cut(s) 488
BsuRI GGCC 7 cut(s) 415, 1052, 1218, 1764, 1998, 2195, 2621
BtgI CCRYGG 1 cut(s) 1065
BtrI CACGTC 1 cut(s) 1561
BtsCI GGATG 8 cut(s) 79, 235, 798, 990, 1048, 1220, 1508, 2455
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 3 cut(s) 75, 933, 1641
BveI ACCTGC 2 cut(s) 194, 1774
Cac8I GCNNGC 8 cut(s) 89, 93, 384, 388, 609, 975, 1781, 1917
CaiI CAGNNNCTG 1 cut(s) 2282
CciI TCATGA 1 cut(s) 2460
CfoI GCGC 4 cut(s) 859, 1483, 1900, 2614
Cfr13I GGNCC 5 cut(s) 283, 1050, 1217, 1323, 1996
Csp6I GTAC 5 cut(s) 83, 1436, 1963, 2302, 2480
CviQI GTAC 5 cut(s) 83, 1436, 1963, 2302, 2480
DdeI CTNAG 5 cut(s) 648, 720, 1227, 1449, 2439
DpnI GATC 8 cut(s) 23, 355, 537, 1032, 1038, 1290, 2149, 2490
DpnII GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
DraI TTTAAA 1 cut(s) 57
EaeI YGGCCR 2 cut(s) 2193, 2619
Eam1104I CTCTTC 3 cut(s) 93, 586, 1020
EarI CTCTTC 3 cut(s) 93, 586, 1020
Ecl136II GAGCTC 2 cut(s) 623, 1928
Eco130I CCWWGG 2 cut(s) 1065, 1327
Eco147I AGGCCT 1 cut(s) 1764
Eco24I GRGCYC 3 cut(s) 625, 1930, 2369
Eco31I GGTCTC 1 cut(s) 517
Eco32I GATATC 1 cut(s) 1540
Eco47I GGWCC 2 cut(s) 283, 1323
Eco53kI GAGCTC 2 cut(s) 623, 1928
Eco57I CTGAAG 3 cut(s) 117, 1403, 2228
Eco81I CCTNAGG 1 cut(s) 720
Eco88I CYCGRG 1 cut(s) 17
Eco91I GGTNACC 1 cut(s) 2180
EcoICRI GAGCTC 2 cut(s) 623, 1928
EcoO109I RGGNCCY 1 cut(s) 1050
EcoO65I GGTNACC 1 cut(s) 2180
EcoRII CCWGG 3 cut(s) 1052, 1533, 2184
EcoRV GATATC 1 cut(s) 1540
EcoT14I CCWWGG 2 cut(s) 1065, 1327
EcoT22I ATGCAT 1 cut(s) 226
EcoT38I GRGCYC 3 cut(s) 625, 1930, 2369
ErhI CCWWGG 2 cut(s) 1065, 1327
FauI CCCGC 1 cut(s) 1598
FauNDI CATATG 1 cut(s) 500
FblI GTMKAC 1 cut(s) 770
Fnu4HI GCNGC 9 cut(s) 156, 612, 741, 1124, 1185, 1605, 1639, 2253, 2517
FokI GGATG 8 cut(s) 86, 242, 785, 977, 1055, 1207, 1495, 2462
FriOI GRGCYC 3 cut(s) 625, 1930, 2369
Fsp4HI GCNGC 9 cut(s) 156, 612, 741, 1124, 1185, 1605, 1639, 2253, 2517
FspBI CTAG 4 cut(s) 911, 1802, 2061, 2492
GlaI GCGC 4 cut(s) 858, 1482, 1899, 2613
GluI GCNGC 9 cut(s) 156, 612, 741, 1124, 1185, 1605, 1639, 2253, 2517
GsaI CCCAGC 2 cut(s) 1519, 2113
GsuI CTGGAG 3 cut(s) 1602, 2396, 2555
HaeIII GGCC 7 cut(s) 415, 1052, 1218, 1764, 1998, 2195, 2621
HapII CCGG 1 cut(s) 458
HhaI GCGC 4 cut(s) 859, 1483, 1900, 2614
Hin6I GCGC 4 cut(s) 857, 1481, 1898, 2612
HinP1I GCGC 4 cut(s) 857, 1481, 1898, 2612
HincII GTYRAC 4 cut(s) 544, 771, 1359, 1397
HindII GTYRAC 4 cut(s) 544, 771, 1359, 1397
HindIII AAGCTT 2 cut(s) 380, 1455
HpaII CCGG 1 cut(s) 458
HphI GGTGA 7 cut(s) 535, 805, 938, 1121, 1675, 2192, 2615
Hpy166II GTNNAC 6 cut(s) 6, 544, 771, 1359, 1397, 2304
Hpy8I GTNNAC 6 cut(s) 6, 544, 771, 1359, 1397, 2304
Hpy99I CGWCG 2 cut(s) 772, 2631
HpyAV CCTTC 6 cut(s) 1040, 1208, 1300, 1326, 1604, 1775
HpyCH4III ACNGT 4 cut(s) 114, 1105, 1411, 2038
HpyCH4IV ACGT 2 cut(s) 676, 1560
HpyF3I CTNAG 5 cut(s) 648, 720, 1227, 1449, 2439
HpySE526I ACGT 2 cut(s) 676, 1560
HspAI GCGC 4 cut(s) 857, 1481, 1898, 2612
Kzo9I GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
LmnI GCTCC 5 cut(s) 1660, 1925, 2118, 2372, 2524
Lsp1109I GCAGC 7 cut(s) 142, 598, 752, 1196, 1616, 1625, 2528
LweI GCATC 7 cut(s) 220, 337, 371, 1567, 1656, 1845, 2379
MaeI CTAG 4 cut(s) 911, 1802, 2061, 2492
MaeII ACGT 2 cut(s) 676, 1560
MaeIII GTNAC 2 cut(s) 1561, 2180
MalI GATC 8 cut(s) 23, 355, 537, 1032, 1038, 1290, 2149, 2490
MboI GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
MflI RGATCY 3 cut(s) 21, 1288, 2488
MhlI GDGCHC 4 cut(s) 625, 1552, 1930, 2369
MlsI TGGCCA 2 cut(s) 2195, 2621
MluNI TGGCCA 2 cut(s) 2195, 2621
MlyI GAGTC 6 cut(s) 199, 1234, 1516, 1558, 1624, 1699
MmeI TCCRAC 1 cut(s) 1301
Mox20I TGGCCA 2 cut(s) 2195, 2621
Mph1103I ATGCAT 1 cut(s) 226
MscI TGGCCA 2 cut(s) 2195, 2621
MseI TTAA 7 cut(s) 56, 320, 969, 1017, 1248, 2361, 2525
MslI CAYNNNNRTG 3 cut(s) 268, 748, 2237
Msp20I TGGCCA 2 cut(s) 2195, 2621
MspA1I CMGCKG 2 cut(s) 560, 1519
MspCI CTTAAG 1 cut(s) 1016
MspI CCGG 1 cut(s) 458
MspR9I CCNGG 3 cut(s) 1054, 1535, 2186
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 3 cut(s) 1054, 1535, 2186
NcoI CCATGG 1 cut(s) 1065
NdeI CATATG 1 cut(s) 500
NdeII GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
NlaIV GGNNCC 5 cut(s) 23, 285, 1051, 1325, 2368
NmuCI GTSAC 2 cut(s) 1561, 2180
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 2 cut(s) 95, 1969
NspV TTCGAA 1 cut(s) 1597
PaeI GCATGC 1 cut(s) 95
PagI TCATGA 1 cut(s) 2460
PceI AGGCCT 1 cut(s) 1764
PciI ACATGT 1 cut(s) 1965
PctI GAATGC 1 cut(s) 226
PfeI GAWTC 4 cut(s) 730, 941, 1496, 1586
PflMI CCANNNNNTGG 2 cut(s) 70, 2419
PfoI TCCNGGA 1 cut(s) 1533
PkrI GCNGC 9 cut(s) 157, 613, 742, 1125, 1186, 1606, 1640, 2254, 2518
PleI GAGTC 6 cut(s) 198, 1234, 1516, 1558, 1623, 1699
PpsI GAGTC 6 cut(s) 198, 1234, 1516, 1558, 1623, 1699
PscI ACATGT 1 cut(s) 1965
PsiI TTATAA 1 cut(s) 630
Psp124BI GAGCTC 2 cut(s) 625, 1930
Psp6I CCWGG 3 cut(s) 1052, 1533, 2184
PspEI GGTNACC 1 cut(s) 2180
PspFI CCCAGC 2 cut(s) 1515, 2109
PspGI CCWGG 3 cut(s) 1052, 1533, 2184
PspN4I GGNNCC 5 cut(s) 23, 285, 1051, 1325, 2368
PspPI GGNCC 5 cut(s) 283, 1050, 1217, 1323, 1996
PstI CTGCAG 2 cut(s) 160, 1606
PstNI CAGNNNCTG 1 cut(s) 2282
PsuI RGATCY 3 cut(s) 21, 1288, 2488
PvuII CAGCTG 2 cut(s) 560, 1519
RsaI GTAC 5 cut(s) 84, 1437, 1964, 2303, 2481
RsaNI GTAC 5 cut(s) 83, 1436, 1963, 2302, 2480
RseI CAYNNNNRTG 3 cut(s) 268, 748, 2237
SacI GAGCTC 2 cut(s) 625, 1930
SalI GTCGAC 1 cut(s) 769
SaqAI TTAA 7 cut(s) 56, 320, 969, 1017, 1248, 2361, 2525
SatI GCNGC 9 cut(s) 156, 612, 741, 1124, 1185, 1605, 1639, 2253, 2517
Sau3AI GATC 8 cut(s) 21, 353, 535, 1030, 1036, 1288, 2147, 2488
Sau96I GGNCC 5 cut(s) 283, 1050, 1217, 1323, 1996
SchI GAGTC 6 cut(s) 199, 1234, 1516, 1558, 1624, 1699
ScrFI CCNGG 3 cut(s) 1054, 1535, 2186
SduI GDGCHC 4 cut(s) 625, 1552, 1930, 2369
SfaNI GCATC 7 cut(s) 220, 337, 371, 1567, 1656, 1845, 2379
SfcI CTRYAG 2 cut(s) 156, 1602
SfuI TTCGAA 1 cut(s) 1597
SinI GGWCC 2 cut(s) 283, 1323
SmiMI CAYNNNNRTG 3 cut(s) 268, 748, 2237
SmlI CTYRAG 5 cut(s) 366, 1016, 1709, 1867, 2117
SmoI CTYRAG 5 cut(s) 366, 1016, 1709, 1867, 2117
SphI GCATGC 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 1764
SsiI CCGC 4 cut(s) 1124, 1591, 2252, 2370
SspI AATATT 5 cut(s) 1060, 1153, 1985, 2082, 2166
SspMI CTAG 4 cut(s) 911, 1802, 2061, 2492
SstI GAGCTC 2 cut(s) 625, 1930
StuI AGGCCT 1 cut(s) 1764
StyD4I CCNGG 3 cut(s) 1052, 1533, 2184
StyI CCWWGG 2 cut(s) 1065, 1327
TaaI ACNGT 4 cut(s) 114, 1105, 1411, 2038
TaiI ACGT 2 cut(s) 679, 1563
TaqI TCGA 8 cut(s) 193, 352, 733, 770, 1491, 1570, 1597, 2211
TatI WGTACW 2 cut(s) 1962, 2301
TauI GCSGC 2 cut(s) 1126, 2255
TfiI GAWTC 4 cut(s) 730, 941, 1496, 1586
Tru1I TTAA 7 cut(s) 56, 320, 969, 1017, 1248, 2361, 2525
Tru9I TTAA 7 cut(s) 56, 320, 969, 1017, 1248, 2361, 2525
TscAI CASTG 3 cut(s) 75, 940, 1648
TseFI GTSAC 2 cut(s) 1561, 2180
TseI GCWGC 7 cut(s) 155, 611, 740, 1184, 1604, 1638, 2516
Tsp45I GTSAC 2 cut(s) 1561, 2180
TspGWI ACGGA 3 cut(s) 686, 1114, 2511
TspRI CASTG 3 cut(s) 75, 940, 1648
Van91I CCANNNNNTGG 2 cut(s) 70, 2419
Vha464I CTTAAG 1 cut(s) 1016
VpaK11BI GGWCC 2 cut(s) 283, 1323
XapI RAATTY 5 cut(s) 1261, 1973, 2122, 2206, 2325
XbaI TCTAGA 1 cut(s) 910
XceI RCATGY 2 cut(s) 95, 1969
XcmI CCANNNNNNNNNTGG 2 cut(s) 71, 408
XmiI GTMKAC 1 cut(s) 770
XspI CTAG 4 cut(s) 911, 1802, 2061, 2492
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.