RchiOBHm_Chr7g0186291

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002290
Physical Location & Seq
Reverse (-)
6272937 .. 6277826
4890 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16628

Sequence Viewer

Length: 2619 bp
ATGGCGATGGAAATTACCCAGTTTCTTTTAAATGCCCAAGCAGTGGATGGAAATGTACGCAAGCATGCTGAAGAGAACATAAGACAGTTTCAAGAGCAAAATCTTCCGAGTTTCCTGTTCTCTCTTGCTGCAGAATTAGCTAATGATGACAAGCCTGTTGAGAGTCGAAAGTTAGCAGGTCTAATTCTAAAGAATGCATTGGATGCCAAAGAACAGCATAGAAAGTTTGAACTTGCACAAAGATGGGTTGCTCTGGACCCAAATGCAAAGACCCAAATTAGAGCGTTCCTGTTAAAGACTTTATCATCTCCTGCCCATGATGCTCGATCAACTGCATCTCAAGTCATTGCAAAGCTTGCAGGCATTGAGTTGCCACTCAAACATTGGCCTGAACTGATAGAAGCTCTGCTGTCAAATAATAATCAGCTTCCGGCTCAAACGAGACAGGCAACTCTTGAAACTCTTGGATACATATGCGAGGAGGTCTCCCCAGAGGTGATAGAGCAAGATCATGTCAACAAAATACTTACAGCTGTTGTTCAGGGCATGAACTCTTCCGAGAGTAATAATGATGTCAGGCTTGCTGCTACACGAGCTCTTTATAATGCTTTGGGTTTTGCTCAGGCGAACTTCTCCAATGATACGGAACGTGATTACATAATGAGAGTTGTATGTGAGGCTACTCTTTCCCCTGAGGTAAAGATTCGACAAGCAGCATTTGAATGTTTGGTTTCTATATCTTCGACATACTATGAGAAATTGTCTCCATACATCCAAGATATTTTCACCATAACTGCAAAGGCTGTCAAGGAAGATGAAGAACCTGTTGCGCTTCAAGCTATTGAGTTTTGGAGTTCAATTTGTGATGAGGAGATTGACATTCTAGAAGAATATGGTGGTGATTTCACTGGGGATTCTGTTATTCCTTGCTTTTATTTTATTAAGCAAGCACTTCCTGCTCTCATCCCCTTGTTATTGGAGACACTTCTTAAGCAGGAAGAGGATCAAGATCAGGATGAAGGGGCCTGGAATATTGCCATGGCTGGTGGCACTTGCTTGGGCTTGGTAGCACGGACAGTTGGTGATGATGTTGTGCCGCTTGTAATGCCATTTATTGAGGAGAATATTTCGAAACCAGATTGGAGGCAGAGGGAGGCAGCCACCTATGCTTTTGGTTCAGTTTTGGAAGGCCCATCCCCTGAGAAGCTAATGACTCTTGTTAATCATGCTTTGAATTTTATGCTAAATGCCCTGATGAAAGACCCAAACAACCATGTGAAGGACACTACTGGTTGGACCCTTGGGAGGATATTTGAGTTTCTACATGGGTCAACTTATGAGACACCCATAATTACCCAAGCAAATTGTCAACAGATTATCACAGTTCTTCTTCAGAGCATGAAAGATGTACCAAATGTTGCTGAGAAAGCCTGTGGTGCTTTATATTTCTTGGCGCAGGGTTTCGAGGATTCAGGGTCTTCATCCACCCAGCTGACTCCCTTTTTCCAGGATATCATCGGGGCACTTCTTGATGTGACTCATCGAGAAGATGCTGGAGAATCCCGCCTTCGAACTGCAGCCTATGAGAGTCTGAATGAAGTTGTAAGGTGCTGCACGGATGAGACTGCTCCAATGGTGATGCAATTAGTTCCTATTATTATGAATGAACTCCACCAGACTCTTGAGGCACATAGGCTTTCATCTGATGAAAGAGACAAGCAGAATGAACTACAAGGCCTTCTCTGTGGTTGCTTGCAGGTCATCATACAAAAGCTAGGGTCATCTGAACCAACTAAATATGTCTTCATGCAGTATGCTGACCAGATGATGCAACTTTTCTTGAGAGTATTTTCTTGTAAAAGTGCCACAGCGCATGAGGAGGCTATGCTTGCCATTGGAGCTCTTGCCTATTCGACTGGCTCTGATTTTGCAAAGTACATGTCTGAATTCTACAAATATTTAGAAATGGGCCTCCAAAACTTTGAGGACTATCAGGTTTGTGCTATAACAGTTGGTGTGGTGGGGGATATATCTAGGGCTTTGGAAGATAAAATATTGCCTTATTGTGATGGGATAATGACCCAGCTCCTCAAGAATTTGTCAAGCAACCAGTTGCACCGATCTGTGAAGCCTCCAATATTTTCATGCTTTGGTGACCTAGCTTTGGCCATTGGGGAGAATTTCGACAAGTATTTGATGTATGCCATGCCCATGCTTCAGAGTGCGGCAGAACTTTCAGTCCAGACATCAGGTGCTGATGATGATATAATAGAGTACACAAATACTTTGAGGAATGGAATTTTGGAGGCATACTCTGGTATCTTACAAGGATTTAAGGGCTCCGCAAAGAGCCAATTATTGATGCCTCATGCACCTCATATTCTCCAGTTCTTGGATAGTTTATACATGGCTAAGGACATGGATGATGTGGTCATGAAGACTGCCATTGGGGTACTTGGAGATCTAGCTGATACCTTGGGTGGTAATGCAGCTCCGTTAATTCAGCAATGTGTATCAAGCAGAGGCTTCCTAAATGAATGTCTCCAGTCAGAGGATAATTTGGTCAAGGAAGCTGGTGAATGGGTGCAGTTGGCCATTCGTCGGGCAATATCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

872

Amino Acids

96.79

Weight (kDa)

4.67

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBN_N PF03810 23 - 102 1e-12 Importin-beta N-terminal domain
HEAT_EZ PF13513 381 - 437 8.9e-10 HEAT-like repeat
TPR_IMB1 PF25574 459 - 830 2.2e-110 Importin subunit beta-1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
Acc36I ACCTGC 2 cut(s) 167, 1747
AccB7I CCANNNNNTGG 2 cut(s) 43, 2392
AciI CCGC 4 cut(s) 1097, 1564, 2225, 2343
AclWI GGATC 1 cut(s) 1011
AcoI YGGCCR 2 cut(s) 2166, 2592
AcsI RAATTY 5 cut(s) 1234, 1946, 2095, 2179, 2298
AcuI CTGAAG 3 cut(s) 90, 1376, 2201
AfaI GTAC 5 cut(s) 57, 1410, 1937, 2276, 2454
AfiI CCNNNNNNNGG 7 cut(s) 43, 1279, 1305, 2164, 2392, 2551, 2601
AflII CTTAAG 1 cut(s) 989
AflIII ACRYGT 1 cut(s) 1938
AgsI TTSAA 7 cut(s) 92, 230, 458, 722, 836, 858, 1234
AjnI CCWGG 2 cut(s) 1025, 1506
AjuI GAANNNNNNNTTGG 2 cut(s) 2285, 2317
AloI GAACNNNNNNTCC 2 cut(s) 2223, 2255
Alw21I GWGCWC 2 cut(s) 598, 1903
Alw26I GTCTC 8 cut(s) 436, 490, 768, 974, 1334, 1616, 1707, 2546
AlwI GGATC 1 cut(s) 1011
AlwNI CAGNNNCTG 1 cut(s) 2255
AoxI GGCC 7 cut(s) 386, 1023, 1189, 1735, 1969, 2166, 2592
ApeKI GCWGC 7 cut(s) 128, 584, 713, 1157, 1577, 1611, 2489
ApoI RAATTY 5 cut(s) 1234, 1946, 2095, 2179, 2298
ArsI GACNNNNNNTTYG 2 cut(s) 1925, 1957
AspLEI GCGC 3 cut(s) 832, 1456, 1873
AspS9I GGNCC 5 cut(s) 256, 1023, 1190, 1296, 1969
AsuHPI GGTGA 7 cut(s) 508, 778, 911, 1094, 1648, 2165, 2588
AsuII TTCGAA 2 cut(s) 1130, 1570
AvaII GGWCC 2 cut(s) 256, 1296
AxyI CCTNAGG 1 cut(s) 693
BaeGI GKGCMC 1 cut(s) 1525
BaeI ACNNNNGTAYC 2 cut(s) 2302, 2335
BalI TGGCCA 2 cut(s) 2168, 2594
BanII GRGCYC 3 cut(s) 598, 1903, 2342
BauI CACGAG 1 cut(s) 591
BbsI GAAGAC 3 cut(s) 1470, 1795, 2444
Bbv12I GWGCWC 2 cut(s) 598, 1903
BbvI GCAGC 7 cut(s) 115, 571, 725, 1169, 1589, 1598, 2501
BccI CCATC 4 cut(s) 41, 237, 1201, 2063
BciT130I CCWGG 2 cut(s) 1027, 1508
BciVI GTATCC 1 cut(s) 461
BcoDI GTCTC 8 cut(s) 436, 490, 768, 974, 1334, 1616, 1707, 2546
BfaI CTAG 5 cut(s) 884, 1775, 2034, 2159, 2465
BfmI CTRYAG 2 cut(s) 129, 1575
BfrI CTTAAG 1 cut(s) 989
BfuAI ACCTGC 2 cut(s) 167, 1747
BfuI GTATCC 1 cut(s) 461
BglII AGATCT 1 cut(s) 2461
BisI GCNGC 9 cut(s) 129, 585, 714, 1097, 1158, 1578, 1612, 2226, 2490
BlsI GCNGC 9 cut(s) 130, 586, 715, 1098, 1159, 1579, 1613, 2227, 2491
Bme1390I CCNGG 2 cut(s) 1027, 1508
Bme18I GGWCC 2 cut(s) 256, 1296
BmgT120I GGNCC 5 cut(s) 256, 1023, 1190, 1296, 1969
BmiI GGNNCC 4 cut(s) 258, 1024, 1298, 2341
BmrFI CCNGG 2 cut(s) 1027, 1508
BmrI ACTGGG 2 cut(s) 13, 918
BmsI GCATC 7 cut(s) 193, 310, 344, 1540, 1629, 1818, 2352
BmuI ACTGGG 2 cut(s) 13, 918
BpiI GAAGAC 3 cut(s) 1470, 1795, 2444
BplI GAGNNNNNCTC 4 cut(s) 470, 502, 2297, 2329
BpmI CTGGAG 3 cut(s) 1575, 2369, 2528
Bpu10I CCTNAGC 2 cut(s) 621, 2412
Bpu14I TTCGAA 2 cut(s) 1130, 1570
BpuEI CTTGAG 4 cut(s) 324, 1703, 1861, 2075
BsaBI GATNNNNATC 1 cut(s) 1008
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 3 cut(s) 1038, 1300, 2475
Bsc4I CCNNNNNNNGG 7 cut(s) 43, 1279, 1305, 2164, 2392, 2551, 2601
Bse1I ACTGG 7 cut(s) 19, 913, 1294, 1921, 2110, 2386, 2545
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 2 cut(s) 345, 2513
Bse8I GATNNNNATC 1 cut(s) 1008
BseBI CCWGG 2 cut(s) 1027, 1508
BseDI CCNNGG 3 cut(s) 1038, 1300, 2475
BseGI GGATG 9 cut(s) 52, 208, 771, 963, 1021, 1193, 1481, 1624, 2428
BseJI GATNNNNATC 1 cut(s) 1008
BseLI CCNNNNNNNGG 7 cut(s) 43, 1279, 1305, 2164, 2392, 2551, 2601
BseMI GCAATG 2 cut(s) 345, 2513
BseMII CTCAG 4 cut(s) 635, 684, 1191, 1413
BseNI ACTGG 7 cut(s) 19, 913, 1294, 1921, 2110, 2386, 2545
BseRI GAGGAG 5 cut(s) 494, 884, 1133, 1892, 2078
BseSI GKGCMC 1 cut(s) 1525
BseXI GCAGC 7 cut(s) 115, 571, 725, 1169, 1589, 1598, 2501
BseYI CCCAGC 2 cut(s) 1488, 2082
BsgI GTGCAG 2 cut(s) 1597, 2606
BshFI GGCC 7 cut(s) 388, 1025, 1191, 1737, 1971, 2168, 2594
BsiHKAI GWGCWC 2 cut(s) 598, 1903
BsiSI CCGG 1 cut(s) 431
BslI CCNNNNNNNGG 7 cut(s) 43, 1279, 1305, 2164, 2392, 2551, 2601
BsmAI GTCTC 8 cut(s) 436, 490, 768, 974, 1334, 1616, 1707, 2546
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 7 cut(s) 388, 1025, 1191, 1737, 1971, 2168, 2594
Bso31I GGTCTC 1 cut(s) 490
Bsp119I TTCGAA 2 cut(s) 1130, 1570
Bsp1286I GDGCHC 4 cut(s) 598, 1525, 1903, 2342
Bsp143I GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 4 cut(s) 1097, 1564, 2225, 2343
BspANI GGCC 7 cut(s) 388, 1025, 1191, 1737, 1971, 2168, 2594
BspCNI CTCAG 4 cut(s) 634, 685, 1192, 1414
BspHI TCATGA 1 cut(s) 2433
BspLI GGNNCC 4 cut(s) 258, 1024, 1298, 2341
BspMAI CTGCAG 2 cut(s) 133, 1579
BspMI ACCTGC 2 cut(s) 167, 1747
BspPI GGATC 1 cut(s) 1011
BspT104I TTCGAA 2 cut(s) 1130, 1570
BspTI CTTAAG 1 cut(s) 989
BspTNI GGTCTC 1 cut(s) 490
BsrDI GCAATG 2 cut(s) 345, 2513
BsrI ACTGG 7 cut(s) 19, 913, 1294, 1921, 2110, 2386, 2545
BssECI CCNNGG 3 cut(s) 1038, 1300, 2475
BssMI GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
BssSI CACGAG 1 cut(s) 591
BssT1I CCWWGG 3 cut(s) 1038, 1300, 2475
Bst2BI CACGAG 1 cut(s) 591
Bst2UI CCWGG 2 cut(s) 1027, 1508
Bst4CI ACNGT 4 cut(s) 87, 1078, 1384, 2011
Bst6I CTCTTC 3 cut(s) 66, 559, 993
BstAFI CTTAAG 1 cut(s) 989
BstAPI GCANNNNNTGC 3 cut(s) 203, 356, 956
BstBI TTCGAA 2 cut(s) 1130, 1570
BstC8I GCNNGC 8 cut(s) 62, 66, 357, 361, 582, 948, 1754, 1890
BstDEI CTNAG 5 cut(s) 621, 693, 1200, 1422, 2412
BstDSI CCRYGG 1 cut(s) 1038
BstEII GGTNACC 1 cut(s) 2153
BstF5I GGATG 9 cut(s) 52, 208, 771, 963, 1021, 1193, 1481, 1624, 2428
BstHHI GCGC 3 cut(s) 832, 1456, 1873
BstKTI GATC 6 cut(s) 329, 511, 1006, 1012, 2123, 2464
BstMAI GTCTC 8 cut(s) 436, 490, 768, 974, 1334, 1616, 1707, 2546
BstMBI GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
BstNI CCWGG 2 cut(s) 1027, 1508
BstNSI RCATGY 2 cut(s) 68, 1942
BstPI GGTNACC 1 cut(s) 2153
BstSCI CCNGG 2 cut(s) 1025, 1506
BstSFI CTRYAG 2 cut(s) 129, 1575
BstSLI GKGCMC 1 cut(s) 1525
BstV1I GCAGC 7 cut(s) 115, 571, 725, 1169, 1589, 1598, 2501
BstV2I GAAGAC 3 cut(s) 1470, 1795, 2444
BstX2I RGATCY 1 cut(s) 2461
BstYI RGATCY 1 cut(s) 2461
Bsu36I CCTNAGG 1 cut(s) 693
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 7 cut(s) 388, 1025, 1191, 1737, 1971, 2168, 2594
BtgI CCRYGG 1 cut(s) 1038
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 9 cut(s) 52, 208, 771, 963, 1021, 1193, 1481, 1624, 2428
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 906
BveI ACCTGC 2 cut(s) 167, 1747
Cac8I GCNNGC 8 cut(s) 62, 66, 357, 361, 582, 948, 1754, 1890
CaiI CAGNNNCTG 1 cut(s) 2255
CciI TCATGA 1 cut(s) 2433
CfoI GCGC 3 cut(s) 832, 1456, 1873
Cfr13I GGNCC 5 cut(s) 256, 1023, 1190, 1296, 1969
Csp6I GTAC 5 cut(s) 56, 1409, 1936, 2275, 2453
CviQI GTAC 5 cut(s) 56, 1409, 1936, 2275, 2453
DdeI CTNAG 5 cut(s) 621, 693, 1200, 1422, 2412
DpnI GATC 6 cut(s) 328, 510, 1005, 1011, 2122, 2463
DpnII GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
DraI TTTAAA 1 cut(s) 30
EaeI YGGCCR 2 cut(s) 2166, 2592
Eam1104I CTCTTC 3 cut(s) 66, 559, 993
EarI CTCTTC 3 cut(s) 66, 559, 993
Ecl136II GAGCTC 2 cut(s) 596, 1901
Eco130I CCWWGG 3 cut(s) 1038, 1300, 2475
Eco147I AGGCCT 1 cut(s) 1737
Eco24I GRGCYC 3 cut(s) 598, 1903, 2342
Eco31I GGTCTC 1 cut(s) 490
Eco32I GATATC 1 cut(s) 1513
Eco47I GGWCC 2 cut(s) 256, 1296
Eco53kI GAGCTC 2 cut(s) 596, 1901
Eco57I CTGAAG 3 cut(s) 90, 1376, 2201
Eco81I CCTNAGG 1 cut(s) 693
Eco91I GGTNACC 1 cut(s) 2153
EcoICRI GAGCTC 2 cut(s) 596, 1901
EcoO109I RGGNCCY 1 cut(s) 1023
EcoO65I GGTNACC 1 cut(s) 2153
EcoRI GAATTC 1 cut(s) 1946
EcoRII CCWGG 2 cut(s) 1025, 1506
EcoRV GATATC 1 cut(s) 1513
EcoT14I CCWWGG 3 cut(s) 1038, 1300, 2475
EcoT22I ATGCAT 1 cut(s) 199
EcoT38I GRGCYC 3 cut(s) 598, 1903, 2342
ErhI CCWWGG 3 cut(s) 1038, 1300, 2475
FauI CCCGC 1 cut(s) 1571
FauNDI CATATG 1 cut(s) 473
Fnu4HI GCNGC 9 cut(s) 129, 585, 714, 1097, 1158, 1578, 1612, 2226, 2490
FokI GGATG 9 cut(s) 59, 215, 758, 950, 1028, 1180, 1468, 1631, 2435
FriOI GRGCYC 3 cut(s) 598, 1903, 2342
Fsp4HI GCNGC 9 cut(s) 129, 585, 714, 1097, 1158, 1578, 1612, 2226, 2490
FspBI CTAG 5 cut(s) 884, 1775, 2034, 2159, 2465
GlaI GCGC 3 cut(s) 831, 1455, 1872
GluI GCNGC 9 cut(s) 129, 585, 714, 1097, 1158, 1578, 1612, 2226, 2490
GsaI CCCAGC 2 cut(s) 1492, 2086
GsuI CTGGAG 3 cut(s) 1575, 2369, 2528
HaeIII GGCC 7 cut(s) 388, 1025, 1191, 1737, 1971, 2168, 2594
HapII CCGG 1 cut(s) 431
HhaI GCGC 3 cut(s) 832, 1456, 1873
Hin6I GCGC 3 cut(s) 830, 1454, 1871
HinP1I GCGC 3 cut(s) 830, 1454, 1871
HincII GTYRAC 3 cut(s) 517, 1332, 1370
HindII GTYRAC 3 cut(s) 517, 1332, 1370
HindIII AAGCTT 1 cut(s) 353
HpaII CCGG 1 cut(s) 431
HphI GGTGA 7 cut(s) 508, 778, 911, 1094, 1648, 2165, 2588
Hpy166II GTNNAC 4 cut(s) 517, 1332, 1370, 2277
Hpy8I GTNNAC 4 cut(s) 517, 1332, 1370, 2277
Hpy99I CGWCG 1 cut(s) 2604
HpyAV CCTTC 5 cut(s) 1013, 1181, 1273, 1577, 1748
HpyCH4III ACNGT 4 cut(s) 87, 1078, 1384, 2011
HpyCH4IV ACGT 1 cut(s) 649
HpyF3I CTNAG 5 cut(s) 621, 693, 1200, 1422, 2412
HpySE526I ACGT 1 cut(s) 649
HspAI GCGC 3 cut(s) 830, 1454, 1871
Kzo9I GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
LmnI GCTCC 5 cut(s) 1633, 1898, 2091, 2345, 2497
Lsp1109I GCAGC 7 cut(s) 115, 571, 725, 1169, 1589, 1598, 2501
LweI GCATC 7 cut(s) 193, 310, 344, 1540, 1629, 1818, 2352
MaeI CTAG 5 cut(s) 884, 1775, 2034, 2159, 2465
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 2 cut(s) 1534, 2153
MalI GATC 6 cut(s) 328, 510, 1005, 1011, 2122, 2463
MboI GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
MflI RGATCY 1 cut(s) 2461
MhlI GDGCHC 4 cut(s) 598, 1525, 1903, 2342
MlsI TGGCCA 2 cut(s) 2168, 2594
MluNI TGGCCA 2 cut(s) 2168, 2594
MlyI GAGTC 6 cut(s) 172, 1207, 1489, 1531, 1597, 1672
MmeI TCCRAC 1 cut(s) 1274
Mox20I TGGCCA 2 cut(s) 2168, 2594
Mph1103I ATGCAT 1 cut(s) 199
MscI TGGCCA 2 cut(s) 2168, 2594
MseI TTAA 7 cut(s) 29, 293, 942, 990, 1221, 2334, 2498
MslI CAYNNNNRTG 3 cut(s) 241, 721, 2210
Msp20I TGGCCA 2 cut(s) 2168, 2594
MspA1I CMGCKG 2 cut(s) 533, 1492
MspCI CTTAAG 1 cut(s) 989
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 2 cut(s) 1027, 1508
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 2 cut(s) 1027, 1508
NcoI CCATGG 1 cut(s) 1038
NdeI CATATG 1 cut(s) 473
NdeII GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
NlaIV GGNNCC 4 cut(s) 258, 1024, 1298, 2341
NmuCI GTSAC 2 cut(s) 1534, 2153
NsiI ATGCAT 1 cut(s) 199
NspI RCATGY 2 cut(s) 68, 1942
NspV TTCGAA 2 cut(s) 1130, 1570
PaeI GCATGC 1 cut(s) 68
PagI TCATGA 1 cut(s) 2433
PceI AGGCCT 1 cut(s) 1737
PciI ACATGT 1 cut(s) 1938
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 4 cut(s) 703, 914, 1469, 1559
PflMI CCANNNNNTGG 2 cut(s) 43, 2392
PfoI TCCNGGA 1 cut(s) 1506
PkrI GCNGC 9 cut(s) 130, 586, 715, 1098, 1159, 1579, 1613, 2227, 2491
PleI GAGTC 6 cut(s) 171, 1207, 1489, 1531, 1596, 1672
PpsI GAGTC 6 cut(s) 171, 1207, 1489, 1531, 1596, 1672
PscI ACATGT 1 cut(s) 1938
PsiI TTATAA 1 cut(s) 603
Psp124BI GAGCTC 2 cut(s) 598, 1903
Psp6I CCWGG 2 cut(s) 1025, 1506
PspEI GGTNACC 1 cut(s) 2153
PspFI CCCAGC 2 cut(s) 1488, 2082
PspGI CCWGG 2 cut(s) 1025, 1506
PspN4I GGNNCC 4 cut(s) 258, 1024, 1298, 2341
PspPI GGNCC 5 cut(s) 256, 1023, 1190, 1296, 1969
PstI CTGCAG 2 cut(s) 133, 1579
PstNI CAGNNNCTG 1 cut(s) 2255
PsuI RGATCY 1 cut(s) 2461
PvuII CAGCTG 2 cut(s) 533, 1492
RsaI GTAC 5 cut(s) 57, 1410, 1937, 2276, 2454
RsaNI GTAC 5 cut(s) 56, 1409, 1936, 2275, 2453
RseI CAYNNNNRTG 3 cut(s) 241, 721, 2210
SacI GAGCTC 2 cut(s) 598, 1903
SaqAI TTAA 7 cut(s) 29, 293, 942, 990, 1221, 2334, 2498
SatI GCNGC 9 cut(s) 129, 585, 714, 1097, 1158, 1578, 1612, 2226, 2490
Sau3AI GATC 6 cut(s) 326, 508, 1003, 1009, 2120, 2461
Sau96I GGNCC 5 cut(s) 256, 1023, 1190, 1296, 1969
SchI GAGTC 6 cut(s) 172, 1207, 1489, 1531, 1597, 1672
ScrFI CCNGG 2 cut(s) 1027, 1508
SduI GDGCHC 4 cut(s) 598, 1525, 1903, 2342
SfaNI GCATC 7 cut(s) 193, 310, 344, 1540, 1629, 1818, 2352
SfcI CTRYAG 2 cut(s) 129, 1575
SfuI TTCGAA 2 cut(s) 1130, 1570
SinI GGWCC 2 cut(s) 256, 1296
SmiMI CAYNNNNRTG 3 cut(s) 241, 721, 2210
SmlI CTYRAG 5 cut(s) 339, 989, 1682, 1840, 2090
SmoI CTYRAG 5 cut(s) 339, 989, 1682, 1840, 2090
SphI GCATGC 1 cut(s) 68
SseBI AGGCCT 1 cut(s) 1737
SsiI CCGC 4 cut(s) 1097, 1564, 2225, 2343
SspI AATATT 5 cut(s) 1033, 1126, 1958, 2055, 2139
SspMI CTAG 5 cut(s) 884, 1775, 2034, 2159, 2465
SstI GAGCTC 2 cut(s) 598, 1903
StuI AGGCCT 1 cut(s) 1737
StyD4I CCNGG 2 cut(s) 1025, 1506
StyI CCWWGG 3 cut(s) 1038, 1300, 2475
TaaI ACNGT 4 cut(s) 87, 1078, 1384, 2011
TaiI ACGT 1 cut(s) 652
TatI WGTACW 2 cut(s) 1935, 2274
TauI GCSGC 2 cut(s) 1099, 2228
TfiI GAWTC 4 cut(s) 703, 914, 1469, 1559
Tru1I TTAA 7 cut(s) 29, 293, 942, 990, 1221, 2334, 2498
Tru9I TTAA 7 cut(s) 29, 293, 942, 990, 1221, 2334, 2498
TscAI CASTG 2 cut(s) 48, 913
TseFI GTSAC 2 cut(s) 1534, 2153
TseI GCWGC 7 cut(s) 128, 584, 713, 1157, 1577, 1611, 2489
Tsp45I GTSAC 2 cut(s) 1534, 2153
TspGWI ACGGA 4 cut(s) 659, 1087, 1631, 2484
TspRI CASTG 2 cut(s) 48, 913
Van91I CCANNNNNTGG 2 cut(s) 43, 2392
Vha464I CTTAAG 1 cut(s) 989
VpaK11BI GGWCC 2 cut(s) 256, 1296
XapI RAATTY 5 cut(s) 1234, 1946, 2095, 2179, 2298
XbaI TCTAGA 1 cut(s) 883
XceI RCATGY 2 cut(s) 68, 1942
XcmI CCANNNNNNNNNTGG 2 cut(s) 44, 381
XspI CTAG 5 cut(s) 884, 1775, 2034, 2159, 2465
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.