Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002290

Physical Location & Seq

Genomic Coordinates Reverse (-)

35522721 .. 35527599

Gene Structure 4879 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1294100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGTATGCAATTTTATACTATACATCGATGGAAGTTACCCAGGTGCTGCTGAATGCTCAATCTATAGATGGAACTGTACGGAATCAAGCTGAAGAGAGTTTGAAACAGTTTCAGGAGCAAGATCCTCCAACGTTCTTGTTGTCTCTTTCTAGAGAGCTAGCAAATGAAGAAAAGCCTGTTGAAAGCCGTAAATTAGCAGGTCTGATACTGAAGAATGCCCTGGATGCTAAAGAACAACCTAGAAAGTTGGAGCTTATTCAAAGATGGCTTGCATTGGAAACTTCTGCAAAAGCGCAGATTAAGATGTTCTTGTTACAGACCCTCGCTTCCCCTGTAGCTGATGCTCGTTCAACTACCTCTCAAGTTATTGCAAAAGTTGCGGGCATTGAGTTGCCCATGAAACAGTGGCCGGAACTGATTGGATCACTATTGTCGAATATTCATCAGTTACCAGCACATGTAAAGCAAGCTACTTTAGAGACTCTTGGGTATTTGTGTGAGGAAATCTCTTCTGATGTCGTGGATCAAGATCAGGTTAATAAAATACTGACAGCTGTAGTTCAGGGTATGAACGCATCTGAAGGGAGCAATGATGTTAGGCTTGCTGCTACCCGAGCACTGTACAATGCTTTGGGTTTTGCTCAGGCCAATTTTAGCAATGATATGGAGCGTGATTATATTATGAGAGTTGTCTGTGAGGCAACTCTGTCCCCTGAAGTGAAGATTCGACAGGCAGCTTTTGAGTGTATAGTCTCCATTTCTTCATCTTACTACGAGAAATTAGCTCCTTACATTCAGGACATATTTAGCATCACAGCAAAAGCTGTAAGGGAAGACCAGGAACCTGTTGCTCTTCAAGCCATTGAATTCTGGAGTTCGATTTGTGATGAGGAGATCGATATATTGGAAAATTATGTGGGTGAATTTAGCGGGGATTCTGATATTCCTTGCTTTTACTTTATCAAGCAGGCAATCCCTGCCCTCGTCCCTATGCTTTTGGAGACGCTTCTTAAGCAGGAAGATGATCAGGAGGAAGATGGGGCTTGGAATATTGCTATGGCTGGGGGGACATGCCTTGGGTTGGTTGCTCGGACAGTGGGAGATGATATTGTTCCACTTGTTATGCCCTTCATCGAAGAAAACATAACAAAACCAGATTGGAGGCAAAGAGAAGCAGCTACTTATGCATTTGGTTCCATCTTGGAGGGTCCTTCGGCAAACAAGCTAACGCCTATTGTAAATGTTGCTTTGACGTTCATGCTCAGTGCTTTAACAAAGGACCCAAACAACCATGTGAAGGACACTACTGCTTGGACATTGGGGAGAATTTTTGAATTCCTTCATGGTTCAACTATGGATACACCCATAATTACCCCAGCAAACTGCCAACAGATCATCACAGTTCTTCTTCAGAGTATGAAAGATGTTCCAAATGTTGCTGAGAAGGCATGCGGTGCTCTCTATTTTCTGGCCCAGGGTTATGAGGATTTTGGCCCATCATCGCCCATTGCCCCTTTTTTCCACGAAATTGTACAGGCTCTACTCACTGTTACTCACAGACCAGATGCTGGGGAATCACGATTAAGGACTGCTGCATATGAAGCGTTGAATGAAGTAGTCAGGTGTTCGACTGATGAAACTGCCCCCATGGTGTTGCAACTTGTTCCTGTTATCATGATTGAACTGCATCAGACTCTTGAGGGCCAGCAGCTTGCATCTGATGAGAAAGAGAGGCAGAGTGAATTACAAGGACTTCTTTGTGGGTGCTTACAGGTCATTATTCAGAAACTAGGTTCCTCAGAGCCCACGAAATATGTGTTCATGCAATACGCAGATCAGATAATGGGACTTTTCTTGAGGGTATTTGCTTGTAGAAGTGCTACTGTCCATGAGGAAGCCATGCTTGCCATTGGGGCCCTTGCATATACAAGTGGCCCGGATTTTGCAAAATACTTGCCAGAGTTTTACAAGTATCTTGAAATGGGGCTTCAAAATTTTGAGGAGTACCAAATCTGTGCTATCACGGTTGGTGTTGTAGGGGATATTTGTAGAGCAGTGGAAGATAAGATTCTGCCTTATTGTGATGGAATTATGACCCAACTTCTCAAGGATCTGTCAAGCAACCAGTTGCACCGCTCTGTAAAGCCTCCTATATTCTCCTGCTTTGGTGATATAGCTCTTGCAATAGGAGAGAACTTTGACAAGTACTTGATGTACGTCATGCCAATGATTCAGAGTGCTGCAGAGATGTCTGCCCACACAGCAGGTGCTGATGATGAAATGACAGAGTACACCAATTCTCTTAGGAACGGAATCTTGGAGGCATATTCTGGGATTTTCCAAGGTTTCAAGAACTCCCCGAAAACGCAGGTTTTGATATCTTATGCTCCGCACATCCTGCAGTTCTTGGATAGCATATACAGGAGGAAAGACATGGATGAAGTGGTGATGAAAACAGCCATTGGTGTCCTTGGAGATCTAGCAGATACACTGGGAAGTAATGCAGGTTCTTTGATACAGCAATCTCAATCGTGCAGAGACTTTTTAAATGAGTGCTTGTCTTCCGAAGACAATTTGATTAAAGAATCTGCAGAATGGGCCAAGATGGCCATCAGTCGTGCCATTTCAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N

Protein Analysis

878

Amino Acids

97.19

Weight (kDa)

4.64

Isoelectric Point (pI)

49.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	30 - 109	5.8e-12	Importin-beta N-terminal domain
HEAT_EZ	PF13513	387 - 443	4e-11	HEAT-like repeat
TPR_IMB1	PF25574	465 - 838	4.9e-110	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002290)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53480
fragaria_vesca	FvH4_5g11510 FvH4_5g11510 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050 FvH4_7g32050
malus_domestica	MD01G1223400.v1.1 MD06G1112600.v1.1 MD07G1294100.v1.1 MD14G1134000.v1.1
prunus_persica	Prupe.2G313900_v2.0.a1 Prupe.5G125800_v2.0.a1
pyrus_communis	pycom01g23260 pycom06g10550 pycom07g26690
rosa_chinensis	RchiOBHm_Chr1g0381661 RchiOBHm_Chr7g0186291
rosa_laevigata	RLG00000004852 RLG00000026217
rosa_multiflora	Rmu_sc0000645.1_g000053 Rmu_sc0012555.1_g000004
rosa_roxburghii	Rroxscaffold_3G00268280
rosa_rugosa	Rorug01G0430300 Rorug01G0430300 Rorug01G0430300 Rorug01G0430300 Rorug01G0430300 Rorug06G0473100 Rorug06G0473100
rosa_samantha	Rh1AG453500 Rh1BG410700 Rh1CG425100 Rh1DG442400 Rh7AG068000 Rh7CG080000 Rh7DG080600
rosa_wichuraiana	Rw1G039560 Rw7G006700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2255
Acc36I	ACCTGC	4 cut(s)	188, 2255, 2359, 2495
AccB7I	CCANNNNNTGG	1 cut(s)	1568
AccBSI	CCGCTC	1 cut(s)	2136
AciI	CCGC	5 cut(s)	380, 930, 1452, 2134, 2390
AclI	AACGTT	1 cut(s)	131
AclWI	GGATC	4 cut(s)	116, 430, 531, 2118
AcoI	YGGCCR	2 cut(s)	407, 2607
AcsI	RAATTY	5 cut(s)	866, 923, 1326, 1334, 1993
AcuI	CTGAAG	5 cut(s)	111, 230, 600, 735, 1394
AfaI	GTAC	7 cut(s)	78, 623, 1533, 2006, 2207, 2216, 2291
AfiI	CCNNNNNNNGG	3 cut(s)	1081, 1297, 1568
AflII	CTTAAG	1 cut(s)	1010
AflIII	ACRYGT	1 cut(s)	457
AjnI	CCWGG	4 cut(s)	39, 219, 837, 1473
AjuI	GAANNNNNNNTTGG	2 cut(s)	2300, 2332
Alw21I	GWGCWC	2 cut(s)	619, 1459
Alw26I	GTCTC	5 cut(s)	147, 473, 757, 995, 2532
AlwI	GGATC	4 cut(s)	116, 430, 531, 2118
AlwNI	CAGNNNCTG	3 cut(s)	46, 1568, 2270
Ama87I	CYCGRG	1 cut(s)	612
AoxI	GGCC	9 cut(s)	407, 645, 1470, 1492, 1702, 1914, 1933, 2598, 2607
ApaI	GGGCCC	1 cut(s)	1918
ApeKI	GCWGC	7 cut(s)	46, 605, 734, 1175, 1592, 1708, 2240
ApoI	RAATTY	5 cut(s)	866, 923, 1326, 1334, 1993
Asp700I	GAANNNNTTC	1 cut(s)	1338
AspLEI	GCGC	1 cut(s)	295
AspS9I	GGNCC	9 cut(s)	1208, 1279, 1471, 1493, 1702, 1914, 1915, 1934, 2598
AsuC2I	CCSGG	1 cut(s)	1937
AsuHPI	GGTGA	3 cut(s)	932, 2180, 2458
AsuNHI	GCTAGC	1 cut(s)	157
AvaI	CYCGRG	1 cut(s)	612
AvaII	GGWCC	2 cut(s)	1208, 1279
BaeGI	GKGCMC	1 cut(s)	1918
BalI	TGGCCA	1 cut(s)	2609
BanII	GRGCYC	2 cut(s)	1806, 1918
BbsI	GAAGAC	3 cut(s)	840, 2553, 2574
Bbv12I	GWGCWC	2 cut(s)	619, 1459
BbvI	GCAGC	7 cut(s)	33, 592, 746, 1187, 1579, 1720, 2227
BccI	CCATC	9 cut(s)	22, 62, 258, 1031, 1205, 1504, 2078, 2599, 2618
BceAI	ACGGC	1 cut(s)	171
BciT130I	CCWGG	4 cut(s)	41, 221, 839, 1475
BciVI	GTATCC	1 cut(s)	1351
BclI	TGATCA	1 cut(s)	1024
BcnI	CCSGG	1 cut(s)	1937
BcoDI	GTCTC	5 cut(s)	147, 473, 757, 995, 2532
BfaI	CTAG	5 cut(s)	150, 158, 240, 1790, 2480
BfmI	CTRYAG	6 cut(s)	63, 333, 555, 2241, 2399, 2589
BfrI	CTTAAG	1 cut(s)	1010
BfuAI	ACCTGC	4 cut(s)	188, 2255, 2359, 2495
BfuI	GTATCC	1 cut(s)	1351
BglI	GCCNNNNNGGC	2 cut(s)	1913, 2606
BglII	AGATCT	1 cut(s)	2476
BisI	GCNGC	7 cut(s)	47, 606, 735, 1176, 1593, 1709, 2241
BlsI	GCNGC	7 cut(s)	48, 607, 736, 1177, 1594, 1710, 2242
BmcAI	AGTACT	1 cut(s)	2207
Bme1390I	CCNGG	5 cut(s)	41, 221, 839, 1475, 1937
Bme18I	GGWCC	2 cut(s)	1208, 1279
BmeT110I	CYCGRG	1 cut(s)	612
BmgT120I	GGNCC	9 cut(s)	1208, 1279, 1471, 1493, 1702, 1914, 1915, 1934, 2598
BmiI	GGNNCC	7 cut(s)	843, 1195, 1209, 1281, 1795, 1915, 1916
BmrFI	CCNGG	5 cut(s)	41, 221, 839, 1475, 1937
BmrI	ACTGGG	1 cut(s)	2501
BmsI	GCATC	7 cut(s)	214, 331, 584, 819, 1555, 1696, 1724
BmtI	GCTAGC	1 cut(s)	161
BmuI	ACTGGG	1 cut(s)	2501
BpiI	GAAGAC	3 cut(s)	840, 2553, 2574
BplI	GAGNNNNNCTC	2 cut(s)	491, 523
BpmI	CTGGAG	1 cut(s)	892
Bpu10I	CCTNAGC	1 cut(s)	642
BpuEI	CTTGAG	4 cut(s)	345, 1718, 1876, 2090
BpuMI	CCSGG	1 cut(s)	1937
Bsa29I	ATCGAT	2 cut(s)	26, 897
BsaBI	GATNNNNATC	2 cut(s)	528, 2609
BsaJI	CCNNGG	8 cut(s)	39, 219, 1075, 1473, 1474, 1647, 2341, 2470
Bsc4I	CCNNNNNNNGG	3 cut(s)	1081, 1297, 1568
Bse1I	ACTGG	2 cut(s)	2125, 2496
Bse3DI	GCAATG	3 cut(s)	595, 664, 1506
Bse8I	GATNNNNATC	2 cut(s)	528, 2609
BseBI	CCWGG	4 cut(s)	41, 221, 839, 1475
BseCI	ATCGAT	2 cut(s)	26, 897
BseDI	CCNNGG	8 cut(s)	39, 219, 1075, 1473, 1474, 1647, 2341, 2470
BseGI	GGATG	3 cut(s)	229, 2394, 2443
BseJI	GATNNNNATC	2 cut(s)	528, 2609
BseLI	CCNNNNNNNGG	3 cut(s)	1081, 1297, 1568
BseMI	GCAATG	3 cut(s)	595, 664, 1506
BseMII	CTCAG	4 cut(s)	656, 1276, 1431, 1812
BseNI	ACTGG	2 cut(s)	2125, 2496
BseRI	GAGGAG	2 cut(s)	905, 2015
BseSI	GKGCMC	1 cut(s)	1918
BseXI	GCAGC	7 cut(s)	33, 592, 746, 1187, 1579, 1720, 2227
BseYI	CCCAGC	3 cut(s)	1061, 1375, 1568
BsgI	GTGCAG	1 cut(s)	2554
BshFI	GGCC	9 cut(s)	409, 647, 1472, 1494, 1704, 1916, 1935, 2600, 2609
BshVI	ATCGAT	2 cut(s)	26, 897
BsiHKAI	GWGCWC	2 cut(s)	619, 1459
BsiHKCI	CYCGRG	1 cut(s)	612
BsiSI	CCGG	2 cut(s)	410, 1937
BslFI	GGGAC	4 cut(s)	694, 971, 1081, 1860
BslI	CCNNNNNNNGG	3 cut(s)	1081, 1297, 1568
BsmAI	GTCTC	5 cut(s)	147, 473, 757, 995, 2532
BsmBI	CGTCTC	1 cut(s)	995
BsmFI	GGGAC	4 cut(s)	694, 971, 1081, 1860
BsmI	GAATGC	2 cut(s)	58, 220
BsnI	GGCC	9 cut(s)	409, 647, 1472, 1494, 1704, 1916, 1935, 2600, 2609
BsoBI	CYCGRG	1 cut(s)	612
Bsp120I	GGGCCC	1 cut(s)	1914
Bsp1286I	GDGCHC	4 cut(s)	619, 1459, 1806, 1918
Bsp1407I	TGTACA	2 cut(s)	621, 1531
Bsp19I	CCATGG	1 cut(s)	1647
BspACI	CCGC	5 cut(s)	380, 930, 1452, 2134, 2390
BspANI	GGCC	9 cut(s)	409, 647, 1472, 1494, 1704, 1916, 1935, 2600, 2609
BspCNI	CTCAG	4 cut(s)	655, 1275, 1432, 1811
BspDI	ATCGAT	2 cut(s)	26, 897
BspHI	TCATGA	1 cut(s)	1674
BspLI	GGNNCC	7 cut(s)	843, 1195, 1209, 1281, 1795, 1915, 1916
BspMAI	CTGCAG	3 cut(s)	2245, 2403, 2593
BspMI	ACCTGC	4 cut(s)	188, 2255, 2359, 2495
BspOI	GCTAGC	1 cut(s)	161
BspPI	GGATC	4 cut(s)	116, 430, 531, 2118
BspQI	GCTCTTC	1 cut(s)	858
BspTI	CTTAAG	1 cut(s)	1010
BsrBI	CCGCTC	1 cut(s)	2136
BsrDI	GCAATG	3 cut(s)	595, 664, 1506
BsrGI	TGTACA	2 cut(s)	621, 1531
BsrI	ACTGG	2 cut(s)	2125, 2496
BssECI	CCNNGG	8 cut(s)	39, 219, 1075, 1473, 1474, 1647, 2341, 2470
BssT1I	CCWWGG	4 cut(s)	1075, 1647, 2341, 2470
Bst2UI	CCWGG	4 cut(s)	41, 221, 839, 1475
Bst4CI	ACNGT	9 cut(s)	76, 108, 405, 621, 1096, 1402, 1549, 1885, 2026
Bst6I	CTCTTC	3 cut(s)	87, 514, 858
BstAFI	CTTAAG	1 cut(s)	1010
BstAPI	GCANNNNNTGC	4 cut(s)	377, 977, 1454, 2398
BstAUI	TGTACA	2 cut(s)	621, 1531
BstDEI	CTNAG	5 cut(s)	642, 1262, 1440, 1798, 2303
BstDSI	CCRYGG	1 cut(s)	1647
BstF5I	GGATG	3 cut(s)	229, 2394, 2443
BstHHI	GCGC	1 cut(s)	295
BstMAI	GTCTC	5 cut(s)	147, 473, 757, 995, 2532
BstNI	CCWGG	4 cut(s)	41, 221, 839, 1475
BstNSI	RCATGY	3 cut(s)	461, 1074, 1452
BstSCI	CCNGG	5 cut(s)	39, 219, 837, 1473, 1935
BstSFI	CTRYAG	6 cut(s)	63, 333, 555, 2241, 2399, 2589
BstSLI	GKGCMC	1 cut(s)	1918
BstV1I	GCAGC	7 cut(s)	33, 592, 746, 1187, 1579, 1720, 2227
BstV2I	GAAGAC	3 cut(s)	840, 2553, 2574
BstX2I	RGATCY	3 cut(s)	121, 2110, 2476
BstYI	RGATCY	3 cut(s)	121, 2110, 2476
Bsu15I	ATCGAT	2 cut(s)	26, 897
BsuI	GTATCC	1 cut(s)	1351
BsuRI	GGCC	9 cut(s)	409, 647, 1472, 1494, 1704, 1916, 1935, 2600, 2609
BsuTUI	ATCGAT	2 cut(s)	26, 897
BtgI	CCRYGG	1 cut(s)	1647
BtgZI	GCGATG	1 cut(s)	1485
BtsCI	GGATG	3 cut(s)	229, 2394, 2443
BtsI	GCAGTG	1 cut(s)	2061
BtsIMutI	CAGTG	7 cut(s)	410, 617, 1101, 1270, 1545, 2061, 2489
BveI	ACCTGC	4 cut(s)	188, 2255, 2359, 2495
CaiI	CAGNNNCTG	3 cut(s)	46, 1568, 2270
CciI	TCATGA	1 cut(s)	1674
CfoI	GCGC	1 cut(s)	295
Cfr13I	GGNCC	9 cut(s)	1208, 1279, 1471, 1493, 1702, 1914, 1915, 1934, 2598
ClaI	ATCGAT	2 cut(s)	26, 897
CseI	GACGC	1 cut(s)	1012
Csp6I	GTAC	7 cut(s)	77, 622, 1532, 2005, 2206, 2215, 2290
CviQI	GTAC	7 cut(s)	77, 622, 1532, 2005, 2206, 2215, 2290
DdeI	CTNAG	5 cut(s)	642, 1262, 1440, 1798, 2303
DraI	TTTAAA	1 cut(s)	2547
EaeI	YGGCCR	2 cut(s)	407, 2607
Eam1104I	CTCTTC	3 cut(s)	87, 514, 858
EarI	CTCTTC	3 cut(s)	87, 514, 858
Eco130I	CCWWGG	4 cut(s)	1075, 1647, 2341, 2470
Eco24I	GRGCYC	2 cut(s)	1806, 1918
Eco32I	GATATC	1 cut(s)	2379
Eco47I	GGWCC	2 cut(s)	1208, 1279
Eco57I	CTGAAG	5 cut(s)	111, 230, 600, 735, 1394
Eco88I	CYCGRG	1 cut(s)	612
EcoO109I	RGGNCCY	4 cut(s)	1208, 1279, 1914, 1915
EcoRI	GAATTC	2 cut(s)	866, 1334
EcoRII	CCWGG	4 cut(s)	39, 219, 837, 1473
EcoRV	GATATC	1 cut(s)	2379
EcoT14I	CCWWGG	4 cut(s)	1075, 1647, 2341, 2470
EcoT22I	ATGCAT	1 cut(s)	1189
EcoT38I	GRGCYC	2 cut(s)	1806, 1918
ErhI	CCWWGG	4 cut(s)	1075, 1647, 2341, 2470
Esp3I	CGTCTC	1 cut(s)	995
FalI	AAGNNNNNCTT	6 cut(s)	293, 325, 1740, 1772, 1887, 1919
FaqI	GGGAC	4 cut(s)	694, 971, 1081, 1860
FauI	CCCGC	2 cut(s)	373, 923
FauNDI	CATATG	1 cut(s)	1597
FbaI	TGATCA	1 cut(s)	1024
Fnu4HI	GCNGC	7 cut(s)	47, 606, 735, 1176, 1593, 1709, 2241
FokI	GGATG	3 cut(s)	236, 2381, 2450
FriOI	GRGCYC	2 cut(s)	1806, 1918
Fsp4HI	GCNGC	7 cut(s)	47, 606, 735, 1176, 1593, 1709, 2241
FspBI	CTAG	5 cut(s)	150, 158, 240, 1790, 2480
GlaI	GCGC	1 cut(s)	294
GluI	GCNGC	7 cut(s)	47, 606, 735, 1176, 1593, 1709, 2241
GsaI	CCCAGC	3 cut(s)	1065, 1379, 1572
GsuI	CTGGAG	1 cut(s)	892
HaeIII	GGCC	9 cut(s)	409, 647, 1472, 1494, 1704, 1916, 1935, 2600, 2609
HapII	CCGG	2 cut(s)	410, 1937
HgaI	GACGC	1 cut(s)	1012
HhaI	GCGC	1 cut(s)	295
Hin6I	GCGC	1 cut(s)	293
HinP1I	GCGC	1 cut(s)	293
HpaII	CCGG	2 cut(s)	410, 1937
HphI	GGTGA	3 cut(s)	932, 2180, 2458
Hpy166II	GTNNAC	1 cut(s)	2292
Hpy8I	GTNNAC	1 cut(s)	2292
HpyAV	CCTTC	6 cut(s)	575, 1138, 1221, 1291, 1349, 1438
HpyCH4III	ACNGT	9 cut(s)	76, 108, 405, 621, 1096, 1402, 1549, 1885, 2026
HpyCH4IV	ACGT	3 cut(s)	131, 1253, 2217
HpyF3I	CTNAG	5 cut(s)	642, 1262, 1440, 1798, 2303
HpySE526I	ACGT	3 cut(s)	131, 1253, 2217
HspAI	GCGC	1 cut(s)	293
Ksp22I	TGATCA	1 cut(s)	1024
LguI	GCTCTTC	1 cut(s)	858
LmnI	GCTCC	6 cut(s)	115, 250, 585, 667, 790, 2392
Lsp1109I	GCAGC	7 cut(s)	33, 592, 746, 1187, 1579, 1720, 2227
LweI	GCATC	7 cut(s)	214, 331, 584, 819, 1555, 1696, 1724
MaeI	CTAG	5 cut(s)	150, 158, 240, 1790, 2480
MaeII	ACGT	3 cut(s)	131, 1253, 2217
MaeIII	GTNAC	4 cut(s)	34, 312, 447, 1549
MbiI	CCGCTC	1 cut(s)	2136
MflI	RGATCY	3 cut(s)	121, 2110, 2476
MhlI	GDGCHC	4 cut(s)	619, 1459, 1806, 1918
MlsI	TGGCCA	1 cut(s)	2609
MluNI	TGGCCA	1 cut(s)	2609
MlyI	GAGTC	2 cut(s)	475, 1687
MmeI	TCCRAC	2 cut(s)	152, 228
Mox20I	TGGCCA	1 cut(s)	2609
Mph1103I	ATGCAT	1 cut(s)	1189
MroXI	GAANNNNTTC	1 cut(s)	1338
MscI	TGGCCA	1 cut(s)	2609
MseI	TTAA	7 cut(s)	300, 537, 1011, 1271, 1583, 2546, 2580
MslI	CAYNNNNRTG	1 cut(s)	2225
Msp20I	TGGCCA	1 cut(s)	2609
MspA1I	CMGCKG	1 cut(s)	554
MspCI	CTTAAG	1 cut(s)	1010
MspI	CCGG	2 cut(s)	410, 1937
MspR9I	CCNGG	5 cut(s)	41, 221, 839, 1475, 1937
Mva1269I	GAATGC	2 cut(s)	58, 220
MvaI	CCWGG	4 cut(s)	41, 221, 839, 1475
NciI	CCSGG	1 cut(s)	1937
NcoI	CCATGG	1 cut(s)	1647
NdeI	CATATG	1 cut(s)	1597
NheI	GCTAGC	1 cut(s)	157
NlaIV	GGNNCC	7 cut(s)	843, 1195, 1209, 1281, 1795, 1915, 1916
NsiI	ATGCAT	1 cut(s)	1189
NspI	RCATGY	3 cut(s)	461, 1074, 1452
PaeI	GCATGC	1 cut(s)	1452
PagI	TCATGA	1 cut(s)	1674
PaqCI	CACCTGC	1 cut(s)	2255
PasI	CCCWGGG	1 cut(s)	1474
PciI	ACATGT	1 cut(s)	457
PciSI	GCTCTTC	1 cut(s)	858
PctI	GAATGC	2 cut(s)	58, 220
PdmI	GAANNNNTTC	1 cut(s)	1338
PfeI	GAWTC	8 cut(s)	82, 724, 935, 1574, 2068, 2230, 2313, 2585
PflMI	CCANNNNNTGG	1 cut(s)	1568
PkrI	GCNGC	7 cut(s)	48, 607, 736, 1177, 1594, 1710, 2242
PleI	GAGTC	2 cut(s)	475, 1687
PpsI	GAGTC	2 cut(s)	475, 1687
PpuMI	RGGWCCY	2 cut(s)	1208, 1279
PscI	ACATGT	1 cut(s)	457
Psp1406I	AACGTT	1 cut(s)	131
Psp5II	RGGWCCY	2 cut(s)	1208, 1279
Psp6I	CCWGG	4 cut(s)	39, 219, 837, 1473
PspFI	CCCAGC	3 cut(s)	1061, 1375, 1568
PspGI	CCWGG	4 cut(s)	39, 219, 837, 1473
PspN4I	GGNNCC	7 cut(s)	843, 1195, 1209, 1281, 1795, 1915, 1916
PspOMI	GGGCCC	1 cut(s)	1914
PspPI	GGNCC	9 cut(s)	1208, 1279, 1471, 1493, 1702, 1914, 1915, 1934, 2598
PspPPI	RGGWCCY	2 cut(s)	1208, 1279
PsrI	GAACNNNNNNTAC	2 cut(s)	2491, 2523
PstI	CTGCAG	3 cut(s)	2245, 2403, 2593
PstNI	CAGNNNCTG	3 cut(s)	46, 1568, 2270
PsuI	RGATCY	3 cut(s)	121, 2110, 2476
PvuII	CAGCTG	1 cut(s)	554
RsaI	GTAC	7 cut(s)	78, 623, 1533, 2006, 2207, 2216, 2291
RsaNI	GTAC	7 cut(s)	77, 622, 1532, 2005, 2206, 2215, 2290
RseI	CAYNNNNRTG	1 cut(s)	2225
SapI	GCTCTTC	1 cut(s)	858
SaqAI	TTAA	7 cut(s)	300, 537, 1011, 1271, 1583, 2546, 2580
SatI	GCNGC	7 cut(s)	47, 606, 735, 1176, 1593, 1709, 2241
Sau96I	GGNCC	9 cut(s)	1208, 1279, 1471, 1493, 1702, 1914, 1915, 1934, 2598
ScaI	AGTACT	1 cut(s)	2207
SchI	GAGTC	2 cut(s)	475, 1687
ScrFI	CCNGG	5 cut(s)	41, 221, 839, 1475, 1937
SduI	GDGCHC	4 cut(s)	619, 1459, 1806, 1918
SfaNI	GCATC	7 cut(s)	214, 331, 584, 819, 1555, 1696, 1724
SfcI	CTRYAG	6 cut(s)	63, 333, 555, 2241, 2399, 2589
SfiI	GGCCNNNNNGGCC	1 cut(s)	2606
SinI	GGWCC	2 cut(s)	1208, 1279
SmiMI	CAYNNNNRTG	1 cut(s)	2225
SmlI	CTYRAG	5 cut(s)	360, 1010, 1697, 1855, 2105
SmoI	CTYRAG	5 cut(s)	360, 1010, 1697, 1855, 2105
SphI	GCATGC	1 cut(s)	1452
SsiI	CCGC	5 cut(s)	380, 930, 1452, 2134, 2390
SspI	AATATT	2 cut(s)	439, 1051
SspMI	CTAG	5 cut(s)	150, 158, 240, 1790, 2480
StyD4I	CCNGG	5 cut(s)	39, 219, 837, 1473, 1935
StyI	CCWWGG	4 cut(s)	1075, 1647, 2341, 2470
TaaI	ACNGT	9 cut(s)	76, 108, 405, 621, 1096, 1402, 1549, 1885, 2026
TaiI	ACGT	3 cut(s)	134, 1256, 2220
TaqI	TCGA	7 cut(s)	26, 434, 727, 878, 897, 1134, 1628
TatI	WGTACW	4 cut(s)	621, 1531, 2205, 2289
TfiI	GAWTC	8 cut(s)	82, 724, 935, 1574, 2068, 2230, 2313, 2585
Tru1I	TTAA	7 cut(s)	300, 537, 1011, 1271, 1583, 2546, 2580
Tru9I	TTAA	7 cut(s)	300, 537, 1011, 1271, 1583, 2546, 2580
TscAI	CASTG	7 cut(s)	410, 624, 1101, 1270, 1552, 2061, 2496
TseI	GCWGC	7 cut(s)	46, 605, 734, 1175, 1592, 1708, 2240
TspGWI	ACGGA	2 cut(s)	94, 2325
TspRI	CASTG	7 cut(s)	410, 624, 1101, 1270, 1552, 2061, 2496
Van91I	CCANNNNNTGG	1 cut(s)	1568
Vha464I	CTTAAG	1 cut(s)	1010
VpaK11BI	GGWCC	2 cut(s)	1208, 1279
XapI	RAATTY	5 cut(s)	866, 923, 1326, 1334, 1993
XbaI	TCTAGA	1 cut(s)	149
XceI	RCATGY	3 cut(s)	461, 1074, 1452
XmnI	GAANNNNTTC	1 cut(s)	1338
XspI	CTAG	5 cut(s)	150, 158, 240, 1790, 2480
ZrmI	AGTACT	1 cut(s)	2207
Zsp2I	ATGCAT	1 cut(s)	1189

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.