MD07G1294200.v1.1

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013165
Physical Location & Seq
Reverse (-)
35529883 .. 35531980
2098 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1294200.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGCGAAGGCAGTGGTCTACGCGCTACTTGCCACCGCCTTTATCTTTCTTCTCGTCATGCTTCCCTCCAAACACCGTGGCCCCAACCACCCCCACCCCCACCTTGGCCTCAACCGACGTCTTGGCTACATGGGTCAATCCCCCATTTTTGACCCTCTGGCAGCCAAGATGGAGTGGTACGCCAAGGAAAGTGGTGGATTCAGAGGGGAAACGAATAGTCGCGTGGGGTTGTCGGAACACAACAACAATCCCGTGATGAATGCGAGTGAGGTGGAAGAAGTGCATGAATATCTCGGCGATGAGGGAAAGTTGAACCTCACGTTGAGGCTGAATACTTTGTTCCCGTTGATAGACCAGGCGCCAAAAGACGGGTTGATTAACTCTAATGAGCTCGAGGATTGGGTCATGAAACAAACCGTGGAGAGGTTGAATTATAGGACGCAGAAAGAAATGGAATCGTATGACAAAGATGGTGATGGAACAATTTCTTTCATGGAGTATCTGCCCAAGTTTTCGACTAAGGATCTAGAGGAAAACGGAATGGAGCACGGAGAAGCTGGATGGTGGAAGCAGCAATTTGATAATGCAGATGCTGATAAGAATGGATTACTTAACTTCAATGAATTTAAAGATTTGTTGCACCCAGAAGACAGTAACAACGAAGAAATACACAAATGGCTGTTGACAGAAAAAATGAAGCGGATGGATCAGGATAACGATGGCAAACTGAATTTCGAGGAGTTTTTACACAACGCTTATGATAATTACAAGACTTACGTTGAATTTGAAAGGGGCGGGAAAAATGTACCCACACCAGAAGAGAAATTTGCAGAACTTGATGTGAACAAGAACAAACTCTTGGAAGTGGAAGAATTGAAACCAATGCTGCATTACATTCACCCGGGAGAATTTTCCTATGCTAAATATTATGCAACCTTTCTGATGTCGGAGGCGGATGATAACAAAGATGGAAAACTAACGCTCGAGGAGATGCTCGATCATGACTATGTTTTCTACAGCACAGTATATGATGACGGCATGAACGAAGATTTTGATGGGGATTTTCACGACGAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

360

Amino Acids

41.72

Weight (kDa)

4.71

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 189 - 250 6.3e-09 EF-hand domain pair
EF-hand_7 PF13499 274 - 332 1.3e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 121
AccB1I GGYRCC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 18
AccII CGCG 2 cut(s) 23, 222
AciI CCGC 4 cut(s) 36, 700, 795, 953
AclWI GGATC 2 cut(s) 531, 714
AcsI RAATTY 6 cut(s) 623, 730, 782, 824, 908, 1073
AcyI GRCGYC 2 cut(s) 118, 359
AfaI GTAC 2 cut(s) 179, 807
AfiI CCNNNNNNNGG 3 cut(s) 104, 368, 423
AgsI TTSAA 6 cut(s) 313, 430, 619, 782, 788, 877
AjnI CCWGG 1 cut(s) 354
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 2 cut(s) 391, 557
AluI AGCT 2 cut(s) 391, 557
Alw21I GWGCWC 2 cut(s) 393, 549
AlwI GGATC 2 cut(s) 531, 714
AlwNI CAGNNNCTG 1 cut(s) 593
Ama87I CYCGRG 3 cut(s) 392, 901, 983
AoxI GGCC 2 cut(s) 79, 106
ApeKI GCWGC 3 cut(s) 161, 571, 886
ApoI RAATTY 6 cut(s) 623, 730, 782, 824, 908, 1073
Asp700I GAANNNNTTC 1 cut(s) 484
AspLEI GCGC 2 cut(s) 25, 361
AspS9I GGNCC 1 cut(s) 80
AsuC2I CCSGG 2 cut(s) 902, 903
AsuHPI GGTGA 2 cut(s) 485, 890
AvaI CYCGRG 3 cut(s) 392, 901, 983
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 2 cut(s) 393, 549
BbvI GCAGC 3 cut(s) 173, 583, 873
BccI CCATC 8 cut(s) 163, 464, 470, 555, 697, 713, 962, 1049
BceAI ACGGC 1 cut(s) 1051
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 2 cut(s) 902, 903
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 1015
BfoI RGCGCY 1 cut(s) 362
BisI GCNGC 3 cut(s) 162, 572, 887
BlsI GCNGC 3 cut(s) 163, 573, 888
Bme1390I CCNGG 3 cut(s) 356, 902, 903
BmeT110I CYCGRG 3 cut(s) 392, 901, 983
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 82, 360
BmrFI CCNGG 3 cut(s) 356, 902, 903
BmsI GCATC 2 cut(s) 580, 981
BpiI GAAGAC 1 cut(s) 654
BpuMI CCSGG 2 cut(s) 902, 903
BsaHI GRCGYC 2 cut(s) 118, 359
BsaJI CCNNGG 5 cut(s) 76, 103, 183, 417, 901
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 368, 423
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 5 cut(s) 76, 103, 183, 417, 901
BseGI GGATG 3 cut(s) 566, 708, 961
BseLI CCNNNNNNNGG 3 cut(s) 104, 368, 423
BseRI GAGGAG 2 cut(s) 752, 1001
BseXI GCAGC 3 cut(s) 173, 583, 873
Bsh1236I CGCG 2 cut(s) 23, 222
BshFI GGCC 2 cut(s) 81, 108
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 393, 549
BsiHKCI CYCGRG 3 cut(s) 392, 901, 983
BsiSI CCGG 1 cut(s) 902
BslI CCNNNNNNNGG 3 cut(s) 104, 368, 423
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 2 cut(s) 81, 108
BsoBI CYCGRG 3 cut(s) 392, 901, 983
Bsp1286I GDGCHC 2 cut(s) 393, 549
Bsp143I GATC 3 cut(s) 523, 706, 997
BspACI CCGC 4 cut(s) 36, 700, 795, 953
BspANI GGCC 2 cut(s) 81, 108
BspFNI CGCG 2 cut(s) 23, 222
BspHI TCATGA 2 cut(s) 405, 1000
BspLI GGNNCC 2 cut(s) 82, 360
BspPI GGATC 2 cut(s) 531, 714
BspT107I GGYRCC 1 cut(s) 358
BssECI CCNNGG 5 cut(s) 76, 103, 183, 417, 901
BssMI GATC 3 cut(s) 523, 706, 997
BssNI GRCGYC 2 cut(s) 118, 359
BssT1I CCWWGG 2 cut(s) 103, 183
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 4 cut(s) 77, 418, 653, 1024
Bst6I CTCTTC 1 cut(s) 813
BstACI GRCGYC 2 cut(s) 118, 359
BstDEI CTNAG 1 cut(s) 519
BstDSI CCRYGG 2 cut(s) 76, 417
BstF5I GGATG 3 cut(s) 566, 708, 961
BstFNI CGCG 2 cut(s) 23, 222
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 2 cut(s) 25, 361
BstKTI GATC 3 cut(s) 526, 709, 1000
BstMBI GATC 3 cut(s) 523, 706, 997
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 354, 900, 901
BstSFI CTRYAG 1 cut(s) 1015
BstUI CGCG 2 cut(s) 23, 222
BstV1I GCAGC 3 cut(s) 173, 583, 873
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 2 cut(s) 81, 108
BtgI CCRYGG 2 cut(s) 76, 417
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 3 cut(s) 566, 708, 961
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
CaiI CAGNNNCTG 1 cut(s) 593
CciI TCATGA 2 cut(s) 405, 1000
CfoI GCGC 2 cut(s) 25, 361
Cfr13I GGNCC 1 cut(s) 80
Cfr9I CCCGGG 1 cut(s) 901
CseI GACGC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 178, 806
CspCI CAANNNNNGTGG 4 cut(s) 58, 93, 172, 207
CviAII CATG 7 cut(s) 58, 130, 284, 406, 493, 1001, 1039
CviJI RGCY 8 cut(s) 81, 108, 126, 164, 328, 391, 557, 679
CviKI_1 RGCY 8 cut(s) 81, 108, 126, 164, 328, 391, 557, 679
CviQI GTAC 2 cut(s) 178, 806
DdeI CTNAG 1 cut(s) 519
DinI GGCGCC 1 cut(s) 360
DpnI GATC 3 cut(s) 525, 708, 999
DpnII GATC 3 cut(s) 523, 706, 997
DraI TTTAAA 1 cut(s) 628
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
EciI GGCGGA 1 cut(s) 968
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 2 cut(s) 103, 183
Eco24I GRGCYC 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 391
Eco88I CYCGRG 3 cut(s) 392, 901, 983
EcoICRI GAGCTC 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 1073
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 2 cut(s) 103, 183
EcoT38I GRGCYC 1 cut(s) 393
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 103, 183
FaeI CATG 7 cut(s) 61, 133, 287, 409, 496, 1004, 1042
FatI CATG 7 cut(s) 57, 129, 283, 405, 492, 1000, 1038
FauI CCCGC 1 cut(s) 788
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 162, 572, 887
FokI GGATG 3 cut(s) 573, 715, 968
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 162, 572, 887
FspBI CTAG 1 cut(s) 527
GlaI GCGC 2 cut(s) 24, 360
GluI GCNGC 3 cut(s) 162, 572, 887
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 2 cut(s) 81, 108
HapII CCGG 1 cut(s) 902
HgaI GACGC 1 cut(s) 448
HhaI GCGC 2 cut(s) 25, 361
Hin1I GRCGYC 2 cut(s) 118, 359
Hin1II CATG 7 cut(s) 61, 133, 287, 409, 496, 1004, 1042
Hin6I GCGC 2 cut(s) 23, 359
HinP1I GCGC 2 cut(s) 23, 359
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 2 cut(s) 198, 455
HpaII CCGG 1 cut(s) 902
HphI GGTGA 2 cut(s) 485, 890
Hpy166II GTNNAC 3 cut(s) 19, 684, 844
Hpy188I TCNGA 5 cut(s) 203, 235, 942, 949, 1079
Hpy188III TCNNGA 5 cut(s) 406, 527, 710, 1001, 1067
Hpy8I GTNNAC 3 cut(s) 19, 684, 844
Hpy99I CGWCG 2 cut(s) 120, 1073
HpyCH4III ACNGT 4 cut(s) 77, 418, 653, 1024
HpyCH4IV ACGT 3 cut(s) 118, 320, 777
HpyCH4V TGCA 6 cut(s) 283, 587, 640, 830, 889, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 1 cut(s) 519
HpySE526I ACGT 3 cut(s) 118, 320, 777
Hsp92I GRCGYC 2 cut(s) 118, 359
Hsp92II CATG 7 cut(s) 61, 133, 287, 409, 496, 1004, 1042
HspAI GCGC 2 cut(s) 23, 359
KasI GGCGCC 1 cut(s) 358
Kzo9I GATC 3 cut(s) 523, 706, 997
LmnI GCTCC 1 cut(s) 544
LpnPI CCDG 8 cut(s) 143, 341, 368, 543, 657, 695, 828, 915
Lsp1109I GCAGC 3 cut(s) 173, 583, 873
LweI GCATC 2 cut(s) 580, 981
MaeI CTAG 1 cut(s) 527
MaeII ACGT 3 cut(s) 118, 320, 777
MaeIII GTNAC 1 cut(s) 653
MalI GATC 3 cut(s) 525, 708, 999
MboI GATC 3 cut(s) 523, 706, 997
MboII GAAGA 7 cut(s) 41, 287, 659, 674, 830, 881, 1058
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 2 cut(s) 393, 549
Mly113I GGCGCC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 213, 927
MroXI GAANNNNTTC 1 cut(s) 484
MseI TTAA 3 cut(s) 378, 612, 627
MslI CAYNNNNRTG 1 cut(s) 1005
MspI CCGG 1 cut(s) 902
MspR9I CCNGG 3 cut(s) 356, 902, 903
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 2 cut(s) 23, 222
MwoI GCNNNNNNNGC 1 cut(s) 29
NarI GGCGCC 1 cut(s) 359
NciI CCSGG 2 cut(s) 902, 903
NdeII GATC 3 cut(s) 523, 706, 997
NlaIII CATG 7 cut(s) 61, 133, 287, 409, 496, 1004, 1042
NlaIV GGNNCC 2 cut(s) 82, 360
NmeAIII GCCGAG 1 cut(s) 273
PaeR7I CTCGAG 2 cut(s) 392, 983
PagI TCATGA 2 cut(s) 405, 1000
PcsI WCGNNNNNNNCGW 1 cut(s) 1041
PctI GAATGC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 2 cut(s) 198, 455
PkrI GCNGC 3 cut(s) 163, 573, 888
PluTI GGCGCC 1 cut(s) 362
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 82, 360
PspPI GGNCC 1 cut(s) 80
PspXI VCTCGAGB 2 cut(s) 392, 983
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 2 cut(s) 179, 807
RsaNI GTAC 2 cut(s) 178, 806
RseI CAYNNNNRTG 1 cut(s) 1005
SacI GAGCTC 1 cut(s) 393
SaqAI TTAA 3 cut(s) 378, 612, 627
SatI GCNGC 3 cut(s) 162, 572, 887
Sau3AI GATC 3 cut(s) 523, 706, 997
Sau96I GGNCC 1 cut(s) 80
ScrFI CCNGG 3 cut(s) 356, 902, 903
SduI GDGCHC 2 cut(s) 393, 549
SfaNI GCATC 2 cut(s) 580, 981
SfcI CTRYAG 1 cut(s) 1015
SfoI GGCGCC 1 cut(s) 360
Sfr274I CTCGAG 2 cut(s) 392, 983
SlaI CTCGAG 2 cut(s) 392, 983
SmaI CCCGGG 1 cut(s) 903
SmiMI CAYNNNNRTG 1 cut(s) 1005
SmlI CTYRAG 2 cut(s) 392, 983
SmoI CTYRAG 2 cut(s) 392, 983
SsiI CCGC 4 cut(s) 36, 700, 795, 953
SspDI GGCGCC 1 cut(s) 358
SspI AATATT 1 cut(s) 926
SspMI CTAG 1 cut(s) 527
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 3 cut(s) 354, 900, 901
StyI CCWWGG 2 cut(s) 103, 183
TaaI ACNGT 4 cut(s) 77, 418, 653, 1024
TaiI ACGT 3 cut(s) 121, 323, 780
TaqI TCGA 5 cut(s) 393, 515, 735, 984, 996
TfiI GAWTC 2 cut(s) 198, 455
Tru1I TTAA 3 cut(s) 378, 612, 627
Tru9I TTAA 3 cut(s) 378, 612, 627
TscAI CASTG 1 cut(s) 18
TseI GCWGC 3 cut(s) 161, 571, 886
TspDTI ATGAA 7 cut(s) 272, 300, 422, 481, 636, 710, 1055
TspGWI ACGGA 2 cut(s) 552, 564
TspMI CCCGGG 1 cut(s) 901
TspRI CASTG 1 cut(s) 18
XapI RAATTY 6 cut(s) 623, 730, 782, 824, 908, 1073
XbaI TCTAGA 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XhoI CTCGAG 2 cut(s) 392, 983
XmaI CCCGGG 1 cut(s) 901
XmiI GTMKAC 1 cut(s) 18
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 1 cut(s) 527
ZraI GACGTC 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.