Rmu_sc0004579.1_g000003

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004579.1
OG0013165
Physical Location & Seq
Reverse (-)
8553 .. 9448
896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004579.1_g000003.1.cds

Sequence Viewer

Length: 711 bp
atggcgaaggcggtggtctatcttctattcgccaccacccttattgctcttatagtcctctctcccttcaagccacggtgccacaacgtccatggcctgcaccgccgccttggcttcaccggccacgtccccattttcgatcctcttgtttcgaagatggaatggtacgtggaggaaaagggattaggagaccaaaacattactctagatacggaacacgacttggttattgccaatgatgttgaggaagcacaggaatatttgagcgaggcgggaacgttgaatataaccatgaggctggtgagtatcttccctttgttggatgtggcacccgaagatggattcgttagctacaatgagctgaagcattggattgtggcacaagccgtggaaagaatgattcataggacacagaatgaaatggcatcccatgatagagatggagatcgagccatttctttcagggagtacctgccacaattttccatcgaggatattgacttaagttcttgtattgttattggggaccaaatgaagcagaatatgtatgcacatatgttccattttttgacagccattgatgatgttaatgataatgaaacagatagaaatgatatggagcatggacaagctggatggtggaagcagcaatttgataacgcagatgctgataggaatggtttacttaccttcaatgaacttaaagagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

236

Amino Acids

27.09

Weight (kDa)

4.77

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 480
AccB1I GGYRCC 2 cut(s) 78, 328
AciI CCGC 4 cut(s) 11, 103, 106, 272
AclI AACGTT 1 cut(s) 278
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 2 cut(s) 167, 470
AfiI CCNNNNNNNGG 2 cut(s) 319, 338
AflII CTTAAG 1 cut(s) 502
AgsI TTSAA 3 cut(s) 70, 283, 694
AjiI CACGTC 1 cut(s) 127
AluBI AGCT 3 cut(s) 351, 361, 632
AluI AGCT 3 cut(s) 351, 361, 632
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 2 cut(s) 94, 121
ApeKI GCWGC 1 cut(s) 646
AspS9I GGNCC 1 cut(s) 526
AsuHPI GGTGA 2 cut(s) 109, 313
AsuII TTCGAA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 526
BanI GGYRCC 2 cut(s) 78, 328
BbvI GCAGC 1 cut(s) 658
BccI CCATC 5 cut(s) 151, 332, 434, 494, 630
BceAI ACGGC 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 206
BfrI CTTAAG 1 cut(s) 502
BfuAI ACCTGC 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 2 cut(s) 106, 647
BlsI GCNGC 2 cut(s) 107, 648
Bme18I GGWCC 1 cut(s) 526
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 3 cut(s) 80, 330, 527
BmsI GCATC 2 cut(s) 434, 655
Bpu14I TTCGAA 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 74, 91, 109, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 338
Bse118I RCCGGY 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 444
BseDI CCNNGG 4 cut(s) 74, 91, 109, 387
BseGI GGATG 3 cut(s) 328, 425, 641
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 319, 338
BseXI GCAGC 1 cut(s) 658
BsgI GTGCAG 1 cut(s) 83
BshFI GGCC 2 cut(s) 96, 123
BshNI GGYRCC 2 cut(s) 78, 328
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 2 cut(s) 113, 539
BslI CCNNNNNNNGG 2 cut(s) 319, 338
BsmAI GTCTC 1 cut(s) 183
BsmFI GGGAC 2 cut(s) 113, 539
BsnI GGCC 2 cut(s) 96, 123
Bso31I GGTCTC 1 cut(s) 183
Bsp119I TTCGAA 1 cut(s) 152
Bsp143I GATC 2 cut(s) 139, 445
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 4 cut(s) 11, 103, 106, 272
BspANI GGCC 2 cut(s) 96, 123
BspLI GGNNCC 3 cut(s) 80, 330, 527
BspMI ACCTGC 1 cut(s) 480
BspPI GGATC 1 cut(s) 134
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 2 cut(s) 78, 328
BspTI CTTAAG 1 cut(s) 502
BspTNI GGTCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 4 cut(s) 74, 91, 109, 387
BssMI GATC 2 cut(s) 139, 445
BssT1I CCWWGG 2 cut(s) 91, 109
Bst4CI ACNGT 1 cut(s) 78
BstAFI CTTAAG 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 169
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 98
BstDSI CCRYGG 3 cut(s) 74, 91, 387
BstF5I GGATG 3 cut(s) 328, 425, 641
BstKTI GATC 2 cut(s) 142, 448
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 2 cut(s) 139, 445
BstMWI GCNNNNNNNGC 3 cut(s) 102, 111, 120
BstV1I GCAGC 1 cut(s) 658
BstXI CCANNNNNNTGG 1 cut(s) 298
BsuRI GGCC 2 cut(s) 96, 123
BtgI CCRYGG 3 cut(s) 74, 91, 387
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 3 cut(s) 328, 425, 641
BveI ACCTGC 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 98
CaiI CAGNNNCTG 1 cut(s) 668
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 526
Csp6I GTAC 2 cut(s) 166, 469
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviAII CATG 4 cut(s) 92, 292, 431, 623
CviQI GTAC 2 cut(s) 166, 469
DpnI GATC 2 cut(s) 141, 447
DpnII GATC 2 cut(s) 139, 445
EaeI YGGCCR 1 cut(s) 121
Eco130I CCWWGG 2 cut(s) 91, 109
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 383
EcoT14I CCWWGG 2 cut(s) 91, 109
ErhI CCWWGG 2 cut(s) 91, 109
FaeI CATG 4 cut(s) 95, 295, 434, 626
FaqI GGGAC 2 cut(s) 113, 539
FatI CATG 4 cut(s) 91, 291, 430, 622
FauI CCCGC 1 cut(s) 265
FauNDI CATATG 1 cut(s) 555
Fnu4HI GCNGC 2 cut(s) 106, 647
FokI GGATG 3 cut(s) 335, 412, 648
Fsp4HI GCNGC 2 cut(s) 106, 647
FspBI CTAG 1 cut(s) 206
GluI GCNGC 2 cut(s) 106, 647
HaeIII GGCC 2 cut(s) 96, 123
HapII CCGG 1 cut(s) 120
Hin1II CATG 4 cut(s) 95, 295, 434, 626
HinfI GANTC 2 cut(s) 342, 400
HpaII CCGG 1 cut(s) 120
HphI GGTGA 2 cut(s) 109, 313
Hpy166II GTNNAC 1 cut(s) 683
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 1 cut(s) 683
HpyAV CCTTC 2 cut(s) 76, 700
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 4 cut(s) 87, 126, 168, 278
HpyCH4V TGCA 2 cut(s) 100, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 111, 120
HpySE526I ACGT 4 cut(s) 87, 126, 168, 278
Hsp92II CATG 4 cut(s) 95, 295, 434, 626
Kzo9I GATC 2 cut(s) 139, 445
LmnI GCTCC 1 cut(s) 619
LpnPI CCDG 7 cut(s) 110, 133, 239, 284, 448, 485, 618
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 2 cut(s) 434, 655
MaeI CTAG 1 cut(s) 206
MaeII ACGT 4 cut(s) 87, 126, 168, 278
MalI GATC 2 cut(s) 141, 447
MboI GATC 2 cut(s) 139, 445
MboII GAAGA 4 cut(s) 14, 166, 301, 347
MluCI AATT 2 cut(s) 479, 650
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 7 cut(s) 68, 153, 166, 238, 262, 288, 484
MseI TTAA 3 cut(s) 503, 588, 702
MspCI CTTAAG 1 cut(s) 502
MspI CCGG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 102, 111, 120
NcoI CCATGG 1 cut(s) 91
NdeI CATATG 1 cut(s) 555
NdeII GATC 2 cut(s) 139, 445
NlaIII CATG 4 cut(s) 95, 295, 434, 626
NlaIV GGNNCC 3 cut(s) 80, 330, 527
NspV TTCGAA 1 cut(s) 152
PfeI GAWTC 2 cut(s) 342, 400
PkrI GCNGC 2 cut(s) 107, 648
Ppu21I YACGTR 1 cut(s) 169
Psp1406I AACGTT 1 cut(s) 278
PspN4I GGNNCC 3 cut(s) 80, 330, 527
PspPI GGNCC 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 668
RsaI GTAC 2 cut(s) 167, 470
RsaNI GTAC 2 cut(s) 166, 469
SaqAI TTAA 3 cut(s) 503, 588, 702
SatI GCNGC 2 cut(s) 106, 647
Sau3AI GATC 2 cut(s) 139, 445
Sau96I GGNCC 1 cut(s) 526
SetI ASST 9 cut(s) 90, 129, 171, 281, 353, 363, 474, 634, 692
SfaNI GCATC 2 cut(s) 434, 655
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 1 cut(s) 526
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
Sse9I AATT 2 cut(s) 479, 650
SsiI CCGC 4 cut(s) 11, 103, 106, 272
SspI AATATT 1 cut(s) 260
SspMI CTAG 1 cut(s) 206
StyI CCWWGG 2 cut(s) 91, 109
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 4 cut(s) 90, 129, 171, 281
TaqI TCGA 4 cut(s) 138, 152, 448, 489
TasI AATT 2 cut(s) 479, 650
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 2 cut(s) 342, 400
Tru1I TTAA 3 cut(s) 503, 588, 702
Tru9I TTAA 3 cut(s) 503, 588, 702
TseI GCWGC 1 cut(s) 646
TspDTI ATGAA 5 cut(s) 392, 432, 548, 612, 711
TspGWI ACGGA 1 cut(s) 227
Vha464I CTTAAG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 526
XbaI TCTAGA 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 2 cut(s) 89, 437
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.