Rh1BG410800

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0013165
Physical Location & Seq
Reverse (-)
53275829 .. 53278140
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG410800.1

Sequence Viewer

Length: 948 bp
ATGGCGAAGGCGGTGGTCTATCTTCTATTCGCCACCACCCTTATTGCTCTTATAGTCCTCTCTCCCTTCAAGCCACGGTGCCACAACGTCCATGGCCTGCACCGCCGCCTTGGCTTCACCGGCCACGTCCCCATTTTCGATCCTCTTGTTTCCAAGATGGAATGGTACGTGGAGGAAAAGGGATTAGGAGACCAAAACATTACTCTAGATACGGAACACGACTTGGTTATTGCCAATGATGTTGAGGAAGCACAGGAATATTTGAGCGAGGCGGGAACGTTGAATATAACCATGAGGCTGGTGAGTATCTTCCCTTTGTTGGATGTGGCACCCGAAGATGGATTCGTTAGCTACAATGAGCTGAAGCATTGGATTGTGGCACAAGCCGTGGAAAGAATGATTCATAGGACACAGAATGAAATGGCATCCCATGATAGAGATGGAGATCGAGCCATTTCTTTCAGGGAGTACCTGCCACAATTTTCCATCGAGGATATTGATAGAAATGATATGGAGCATGGACAAGCTGGATGGTGGAAGCAGCAATTTGATAACGCAGATGCTGATAGGAATGGTTTACTTACCTTCAATGAACTTAAAGATTTTTTGCACCCAGAAGACAGTTCAAATGACAGAATTCATAAATGGCTCTTGACAGAAAAAATGAAAAGGATGGACCATGATAATGATGGCAAGCTGAACTTCCAGGAGTTTTCGGACAATGCTTATGATATCTTCAAGAATTATGTTGAATTTGAGACAGGCGGAGTACCCACAGCAGAAGATAAATTTGCAGAGCTTGATGTGAACAAAAACAAATTCATAGAAGCTGAAGAATTGAAACCCATACTGCATTATCTTTACCCAGGAGAACTAACCTATGCTACCTATTTCACAAGTTTTCTAATTCATGAGGTCAGTTTGAGTGCAGTACACACTGTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

315

Amino Acids

36.41

Weight (kDa)

4.87

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 179 - 239 2.3e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 480
AccB1I GGYRCC 2 cut(s) 78, 328
AciI CCGC 5 cut(s) 11, 103, 106, 272, 765
AclI AACGTT 1 cut(s) 278
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 4 cut(s) 636, 752, 788, 818
AcuI CTGAAG 2 cut(s) 383, 852
AfaI GTAC 4 cut(s) 167, 470, 771, 933
AfiI CCNNNNNNNGG 2 cut(s) 319, 338
AgsI TTSAA 7 cut(s) 70, 283, 589, 627, 739, 752, 841
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 2 cut(s) 705, 865
AluBI AGCT 6 cut(s) 351, 361, 527, 697, 799, 830
AluI AGCT 6 cut(s) 351, 361, 527, 697, 799, 830
Alw26I GTCTC 2 cut(s) 183, 752
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 2 cut(s) 94, 121
ApeKI GCWGC 1 cut(s) 541
ApoI RAATTY 4 cut(s) 636, 752, 788, 818
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 2 cut(s) 109, 313
AvaII GGWCC 1 cut(s) 676
BanI GGYRCC 2 cut(s) 78, 328
BbsI GAAGAC 1 cut(s) 624
BbvI GCAGC 1 cut(s) 553
BccI CCATC 7 cut(s) 151, 332, 434, 494, 525, 667, 683
BceAI ACGGC 1 cut(s) 371
BciT130I CCWGG 2 cut(s) 707, 867
BcoDI GTCTC 2 cut(s) 183, 752
BfaI CTAG 1 cut(s) 206
BfmI CTRYAG 1 cut(s) 939
BfuAI ACCTGC 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 2 cut(s) 106, 542
BlsI GCNGC 2 cut(s) 107, 543
Bme1390I CCNGG 2 cut(s) 707, 867
Bme18I GGWCC 1 cut(s) 676
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 2 cut(s) 80, 330
BmrFI CCNGG 2 cut(s) 707, 867
BmsI GCATC 2 cut(s) 434, 550
BpiI GAAGAC 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 74, 91, 109, 387, 865
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 338
Bse118I RCCGGY 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 2 cut(s) 707, 867
BseDI CCNNGG 5 cut(s) 74, 91, 109, 387, 865
BseGI GGATG 4 cut(s) 328, 425, 536, 678
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 319, 338
BseXI GCAGC 1 cut(s) 553
BsgI GTGCAG 2 cut(s) 83, 948
BshFI GGCC 2 cut(s) 96, 123
BshNI GGYRCC 2 cut(s) 78, 328
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 2 cut(s) 319, 338
BsmAI GTCTC 2 cut(s) 183, 752
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 2 cut(s) 96, 123
Bso31I GGTCTC 1 cut(s) 183
Bsp143I GATC 2 cut(s) 139, 445
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 5 cut(s) 11, 103, 106, 272, 765
BspANI GGCC 2 cut(s) 96, 123
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 2 cut(s) 80, 330
BspMI ACCTGC 1 cut(s) 480
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 2 cut(s) 78, 328
BspTNI GGTCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 5 cut(s) 74, 91, 109, 387, 865
BssMI GATC 2 cut(s) 139, 445
BssT1I CCWWGG 2 cut(s) 91, 109
Bst2UI CCWGG 2 cut(s) 707, 867
Bst4CI ACNGT 3 cut(s) 78, 623, 940
BstBAI YACGTR 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 98, 695
BstDSI CCRYGG 3 cut(s) 74, 91, 387
BstF5I GGATG 4 cut(s) 328, 425, 536, 678
BstKTI GATC 2 cut(s) 142, 448
BstMAI GTCTC 2 cut(s) 183, 752
BstMBI GATC 2 cut(s) 139, 445
BstMWI GCNNNNNNNGC 3 cut(s) 102, 111, 120
BstNI CCWGG 2 cut(s) 707, 867
BstSCI CCNGG 2 cut(s) 705, 865
BstSFI CTRYAG 1 cut(s) 939
BstV1I GCAGC 1 cut(s) 553
BstV2I GAAGAC 1 cut(s) 624
BstXI CCANNNNNNTGG 1 cut(s) 298
BsuRI GGCC 2 cut(s) 96, 123
BtgI CCRYGG 3 cut(s) 74, 91, 387
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 4 cut(s) 328, 425, 536, 678
BtsIMutI CAGTG 1 cut(s) 936
BveI ACCTGC 1 cut(s) 480
Cac8I GCNNGC 2 cut(s) 98, 695
CaiI CAGNNNCTG 1 cut(s) 563
CciI TCATGA 1 cut(s) 910
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 676
Csp6I GTAC 4 cut(s) 166, 469, 770, 932
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviAII CATG 6 cut(s) 92, 292, 431, 518, 680, 911
CviQI GTAC 4 cut(s) 166, 469, 770, 932
DpnI GATC 2 cut(s) 141, 447
DpnII GATC 2 cut(s) 139, 445
EaeI YGGCCR 1 cut(s) 121
EciI GGCGGA 1 cut(s) 780
Eco130I CCWWGG 2 cut(s) 91, 109
Eco31I GGTCTC 1 cut(s) 183
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 1 cut(s) 676
Eco57I CTGAAG 2 cut(s) 383, 852
EcoRI GAATTC 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 705, 865
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 2 cut(s) 91, 109
ErhI CCWWGG 2 cut(s) 91, 109
FaeI CATG 6 cut(s) 95, 295, 434, 521, 683, 914
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FaqI GGGAC 1 cut(s) 113
FatI CATG 6 cut(s) 91, 291, 430, 517, 679, 910
FauI CCCGC 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 106, 542
FokI GGATG 4 cut(s) 335, 412, 543, 685
Fsp4HI GCNGC 2 cut(s) 106, 542
FspBI CTAG 1 cut(s) 206
GluI GCNGC 2 cut(s) 106, 542
HaeIII GGCC 2 cut(s) 96, 123
HapII CCGG 1 cut(s) 120
Hin1II CATG 6 cut(s) 95, 295, 434, 521, 683, 914
HinfI GANTC 2 cut(s) 342, 400
HpaII CCGG 1 cut(s) 120
HphI GGTGA 2 cut(s) 109, 313
Hpy166II GTNNAC 3 cut(s) 578, 808, 934
Hpy188I TCNGA 1 cut(s) 718
Hpy188III TCNNGA 4 cut(s) 206, 652, 739, 911
Hpy8I GTNNAC 3 cut(s) 578, 808, 934
HpyAV CCTTC 2 cut(s) 76, 595
HpyCH4III ACNGT 3 cut(s) 78, 623, 940
HpyCH4IV ACGT 4 cut(s) 87, 126, 168, 278
HpyCH4V TGCA 5 cut(s) 100, 610, 794, 853, 929
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 111, 120
HpySE526I ACGT 4 cut(s) 87, 126, 168, 278
Hsp92II CATG 6 cut(s) 95, 295, 434, 521, 683, 914
Kzo9I GATC 2 cut(s) 139, 445
LmnI GCTCC 1 cut(s) 514
Lsp1109I GCAGC 1 cut(s) 553
LweI GCATC 2 cut(s) 434, 550
MaeI CTAG 1 cut(s) 206
MaeII ACGT 4 cut(s) 87, 126, 168, 278
MalI GATC 2 cut(s) 141, 447
MboI GATC 2 cut(s) 139, 445
MboII GAAGA 7 cut(s) 14, 301, 347, 629, 727, 794, 845
MluCI AATT 9 cut(s) 479, 545, 636, 742, 752, 788, 818, 836, 906
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 8 cut(s) 68, 153, 166, 238, 262, 288, 484, 907
MseI TTAA 1 cut(s) 597
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 707, 867
MvaI CCWGG 2 cut(s) 707, 867
MwoI GCNNNNNNNGC 3 cut(s) 102, 111, 120
NcoI CCATGG 1 cut(s) 91
NdeII GATC 2 cut(s) 139, 445
NlaIII CATG 6 cut(s) 95, 295, 434, 521, 683, 914
NlaIV GGNNCC 2 cut(s) 80, 330
PagI TCATGA 1 cut(s) 910
PfeI GAWTC 2 cut(s) 342, 400
PfoI TCCNGGA 1 cut(s) 705
PkrI GCNGC 2 cut(s) 107, 543
Ppu21I YACGTR 1 cut(s) 169
Psp1406I AACGTT 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 705, 865
PspGI CCWGG 2 cut(s) 705, 865
PspN4I GGNNCC 2 cut(s) 80, 330
PspPI GGNCC 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 563
RsaI GTAC 4 cut(s) 167, 470, 771, 933
RsaNI GTAC 4 cut(s) 166, 469, 770, 932
RseI CAYNNNNRTG 1 cut(s) 684
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 2 cut(s) 106, 542
Sau3AI GATC 2 cut(s) 139, 445
Sau96I GGNCC 1 cut(s) 676
ScrFI CCNGG 2 cut(s) 707, 867
SfaNI GCATC 2 cut(s) 434, 550
SfcI CTRYAG 1 cut(s) 939
SinI GGWCC 1 cut(s) 676
SmiMI CAYNNNNRTG 1 cut(s) 684
Sse9I AATT 9 cut(s) 479, 545, 636, 742, 752, 788, 818, 836, 906
SsiI CCGC 5 cut(s) 11, 103, 106, 272, 765
SspI AATATT 1 cut(s) 260
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 705, 865
StyI CCWWGG 2 cut(s) 91, 109
TaaI ACNGT 3 cut(s) 78, 623, 940
TaiI ACGT 4 cut(s) 90, 129, 171, 281
TaqI TCGA 3 cut(s) 138, 448, 489
TasI AATT 9 cut(s) 479, 545, 636, 742, 752, 788, 818, 836, 906
TatI WGTACW 1 cut(s) 931
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 2 cut(s) 342, 400
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TscAI CASTG 1 cut(s) 943
TseI GCWGC 1 cut(s) 541
TspDTI ATGAA 7 cut(s) 392, 432, 606, 629, 680, 811, 899
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 943
VpaK11BI GGWCC 1 cut(s) 676
XapI RAATTY 4 cut(s) 636, 752, 788, 818
XbaI TCTAGA 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 3 cut(s) 89, 437, 686
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.