Rroxscaffold_4G00278530

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013165
Physical Location & Seq
Forward (+)
1484879 .. 1485445
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00278530.1

Sequence Viewer

Length: 369 bp
ATGGACCATGATAATGATGGCAAGCTGAACTTCCAGGAGTTTTCGGACAATGCTTATGATATCTTCAAGAATTATGTTGAATTTGAGACAGGCGGAGTACCCACAGCAGAAGATAAATTTGTTGAGCTTGATGTGAACAAAAACAAATTCATAGAAGCTGAAGAATTGAAACCCATACTGCATTATCTTTACCCAGGAGAACTAACCTATGCTACCTATTTCACAAGTTTTCTAATTCATGAGGCTGATGATAACAAAGATGAAAAATTAACGTTGCAAGAGATGCTGCATCACGAAGATATTTTCTACAACACAGTCTATGAGAGCAGCAATGGCGATGACAACCATGATGATCACGACGAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

122

Amino Acids

14.4

Weight (kDa)

4.22

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 37 - 97 2.4e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 93
AclI AACGTT 1 cut(s) 272
AcsI RAATTY 4 cut(s) 80, 116, 146, 362
AcuI CTGAAG 1 cut(s) 180
AfaI GTAC 1 cut(s) 99
AgsI TTSAA 3 cut(s) 67, 80, 169
AjnI CCWGG 2 cut(s) 33, 193
AluBI AGCT 3 cut(s) 25, 127, 158
AluI AGCT 3 cut(s) 25, 127, 158
Alw26I GTCTC 1 cut(s) 80
ApeKI GCWGC 2 cut(s) 286, 327
ApoI RAATTY 4 cut(s) 80, 116, 146, 362
AspS9I GGNCC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 273, 339
BccI CCATC 1 cut(s) 11
BciT130I CCWGG 2 cut(s) 35, 195
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 80
BisI GCNGC 2 cut(s) 287, 328
BlsI GCNGC 2 cut(s) 288, 329
Bme1390I CCNGG 2 cut(s) 35, 195
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 2 cut(s) 35, 195
BmsI GCATC 2 cut(s) 273, 298
BsaJI CCNNGG 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 2 cut(s) 35, 195
BseDI CCNNGG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 337
BseXI GCAGC 2 cut(s) 273, 339
BsmAI GTCTC 1 cut(s) 80
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 1 cut(s) 93
BspHI TCATGA 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 1 cut(s) 352
Bst2UI CCWGG 2 cut(s) 35, 195
Bst4CI ACNGT 1 cut(s) 316
BstAPI GCANNNNNTGC 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 23
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 2 cut(s) 283, 333
BstNI CCWGG 2 cut(s) 35, 195
BstSCI CCNGG 2 cut(s) 33, 193
BstV1I GCAGC 2 cut(s) 273, 339
BtgZI GCGATG 1 cut(s) 351
Cac8I GCNNGC 1 cut(s) 23
CciI TCATGA 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 98
CviAII CATG 3 cut(s) 8, 239, 347
CviJI RGCY 4 cut(s) 25, 127, 158, 245
CviKI_1 RGCY 4 cut(s) 25, 127, 158, 245
CviQI GTAC 1 cut(s) 98
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
EciI GGCGGA 1 cut(s) 108
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 180
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 33, 193
EcoRV GATATC 1 cut(s) 61
FaeI CATG 3 cut(s) 11, 242, 350
FaiI YATR 9 cut(s) 9, 57, 75, 152, 176, 210, 240, 321, 348
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 3 cut(s) 7, 238, 346
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 2 cut(s) 287, 328
Fsp4HI GCNGC 2 cut(s) 287, 328
GluI GCNGC 2 cut(s) 287, 328
Hin1II CATG 3 cut(s) 11, 242, 350
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 4 cut(s) 67, 239, 293, 356
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 362
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 3 cut(s) 181, 277, 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 333
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 3 cut(s) 11, 242, 350
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 1 cut(s) 352
LpnPI CCDG 5 cut(s) 20, 47, 75, 180, 207
Lsp1109I GCAGC 2 cut(s) 273, 339
LweI GCATC 2 cut(s) 273, 298
MaeII ACGT 1 cut(s) 272
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 4 cut(s) 55, 122, 173, 308
MluCI AATT 8 cut(s) 70, 80, 116, 146, 164, 234, 266, 362
MnlI CCTC 1 cut(s) 235
MseI TTAA 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 12
MspR9I CCNGG 2 cut(s) 35, 195
MvaI CCWGG 2 cut(s) 35, 195
MwoI GCNNNNNNNGC 2 cut(s) 283, 333
NdeII GATC 1 cut(s) 352
NlaIII CATG 3 cut(s) 11, 242, 350
PagI TCATGA 1 cut(s) 238
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 2 cut(s) 288, 329
Psp1406I AACGTT 1 cut(s) 272
Psp6I CCWGG 2 cut(s) 33, 193
PspGI CCWGG 2 cut(s) 33, 193
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
RseI CAYNNNNRTG 1 cut(s) 12
SaqAI TTAA 1 cut(s) 269
SatI GCNGC 2 cut(s) 287, 328
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 35, 195
SetI ASST 6 cut(s) 27, 129, 160, 209, 218, 275
SfaNI GCATC 2 cut(s) 273, 298
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 12
Sse9I AATT 8 cut(s) 70, 80, 116, 146, 164, 234, 266, 362
SsiI CCGC 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 33, 193
TaaI ACNGT 1 cut(s) 316
TaiI ACGT 1 cut(s) 275
TasI AATT 8 cut(s) 70, 80, 116, 146, 164, 234, 266, 362
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TseI GCWGC 2 cut(s) 286, 327
TspDTI ATGAA 3 cut(s) 139, 227, 276
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 4 cut(s) 80, 116, 146, 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.