MD01G1223500.v1.1

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013165
Physical Location & Seq
Reverse (-)
31521051 .. 31522942
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1223500.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGCGAAGGCAGTGGTCTACGCGCTACTTGCCACCGCCTTTATCTTTCTTCTCGTCATGCTTCCCTCCAAACAACGTGGCCCCAACCACCGCCGCCGCCACCACCACCCCCACCCCCTCCTCGGCCTTAACCGCCGTCTTGGCTACACGGGTCATGCTCCCATTTTTGACCCTCTGGTCGCCAAGATGGAGAGGTACGCCAAGGAAAATGGATTCCCAGGGGGAATGAATGCAAGTGAGGTGGAAGAAGTACATGAATATATTGGCAATGAGGGAAAGTTGAATCTCACGTTGAGGCTGATTAATCTGTTCCCGTTGATAGACCAGGCGCCAAAAGACGGGTTCATTAGCTCTAAGGAGCTCGAGCAATGGGTCATGCAACAAACCTTGGAGAGGTTGAATTATAGCACACAGAAAGAAATGGAATCGCACGATAAAGATGGTGATGGAACAATTTCTTTCGCGGAGTATCTGCCCAAGTTTTCCACTAAGGATCTAGCGAAAAACAAAATGGAGCACGGAGAAGCTGGATGGTGGAAGCAACAATTTGAGAATGCAGATGCTGATAAGAATGGATTACTAAACTTCAATGAATTGAAAGATTTGTTGCACCCAGAAGACAGCAACAATGAAGAAATTCACAAATGGCTGTTGAGAGAAAAAATGAAGCGGATGGACAAGGATAAGGATGGGAAAATGAATTTCATGGAGTTTTTACACAACGCTTATGATAATTACAAGAGTTACGTAGAATTTGAAACGGGCGGGAAGAATGTACCCTCGCCAGAACAGAAATTTACAGAACTTGATGTGAACAAGAACAAACACTTGGAAGTGGAAGAATTGAAACCAATCCTACATTACATTCACCCAGGAGAATTGTCCTATGCTAAATATTATGCAAGCTTTCTGATGTTGGAGGCGGATGGTAACAAAGATGGAAAATTAACGCTCGAGGAGATGCTCGATCATGATTATGTTTTCTACAACACAGTCTATGATGGCAGCATCAACGAAAATCTTGATGAAGATTTGCATGACGAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

350

Amino Acids

40.59

Weight (kDa)

5.41

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 179 - 240 3.1e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27790 AT4G27790
fragaria_vesca FvH4_7g32060
malus_domestica MD01G1223500.v1.1 MD07G1294200.v1.1
prunus_persica Prupe.2G314000_v2.0.a1
pyrus_communis pycom07g26700
rosa_chinensis RchiOBHm_Chr1g0381671
rosa_laevigata RLG00000026216
rosa_multiflora Rmu_sc0004579.1_g000002 Rmu_sc0004579.1_g000003
rosa_roxburghii Rroxscaffold_4G00278530
rosa_rugosa Rorug01G0430300
rosa_samantha Rh1AG453600 Rh1BG410800 Rh1CG425200 Rh1DG442500
rosa_wichuraiana Rw1G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 328
AccI GTMKAC 1 cut(s) 18
AccII CGCG 2 cut(s) 23, 464
AciI CCGC 9 cut(s) 36, 91, 94, 97, 133, 464, 670, 765, 923
AclWI GGATC 1 cut(s) 501
AcsI RAATTY 5 cut(s) 636, 700, 752, 794, 1043
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 3 cut(s) 197, 252, 777
AfiI CCNNNNNNNGG 3 cut(s) 122, 338, 393
AgsI TTSAA 6 cut(s) 283, 400, 589, 598, 758, 847
AjnI CCWGG 3 cut(s) 217, 324, 871
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
AluBI AGCT 4 cut(s) 351, 361, 527, 906
AluI AGCT 4 cut(s) 351, 361, 527, 906
Alw21I GWGCWC 2 cut(s) 363, 519
AlwI GGATC 1 cut(s) 501
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 2 cut(s) 362, 953
AoxI GGCC 2 cut(s) 79, 124
ApeKI GCWGC 1 cut(s) 1005
ApoI RAATTY 5 cut(s) 636, 700, 752, 794, 1043
AseI ATTAAT 1 cut(s) 303
Asp700I GAANNNNTTC 2 cut(s) 454, 636
AspLEI GCGC 2 cut(s) 25, 331
AspS9I GGNCC 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 455, 860
AvaI CYCGRG 2 cut(s) 362, 953
BanI GGYRCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 363, 519
BbvI GCAGC 1 cut(s) 1017
BccI CCATC 9 cut(s) 181, 434, 440, 525, 667, 683, 920, 932, 995
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 3 cut(s) 219, 326, 873
BfaI CTAG 1 cut(s) 497
BfoI RGCGCY 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 141
BisI GCNGC 3 cut(s) 94, 97, 1006
BlsI GCNGC 3 cut(s) 95, 98, 1007
Bme1390I CCNGG 3 cut(s) 219, 326, 873
BmeT110I CYCGRG 2 cut(s) 362, 953
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 82, 330
BmrFI CCNGG 3 cut(s) 219, 326, 873
BmsI GCATC 3 cut(s) 550, 951, 1017
BpiI GAAGAC 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 748
BsaHI GRCGYC 1 cut(s) 329
BsaJI CCNNGG 6 cut(s) 121, 201, 217, 218, 387, 871
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 338, 393
Bse3DI GCAATG 2 cut(s) 274, 374
BseBI CCWGG 3 cut(s) 219, 326, 873
BseDI CCNNGG 6 cut(s) 121, 201, 217, 218, 387, 871
BseGI GGATG 4 cut(s) 536, 678, 694, 931
BseLI CCNNNNNNNGG 3 cut(s) 122, 338, 393
BseMI GCAATG 2 cut(s) 274, 374
BseRI GAGGAG 2 cut(s) 110, 971
BseXI GCAGC 1 cut(s) 1017
Bsh1236I CGCG 2 cut(s) 23, 464
BshFI GGCC 2 cut(s) 81, 126
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 2 cut(s) 363, 519
BsiHKCI CYCGRG 2 cut(s) 362, 953
BslI CCNNNNNNNGG 3 cut(s) 122, 338, 393
BsmI GAATGC 2 cut(s) 235, 559
BsnI GGCC 2 cut(s) 81, 126
BsoBI CYCGRG 2 cut(s) 362, 953
Bsp1286I GDGCHC 2 cut(s) 363, 519
Bsp143I GATC 2 cut(s) 493, 967
BspACI CCGC 9 cut(s) 36, 91, 94, 97, 133, 464, 670, 765, 923
BspANI GGCC 2 cut(s) 81, 126
BspFNI CGCG 2 cut(s) 23, 464
BspHI TCATGA 1 cut(s) 970
BspLI GGNNCC 2 cut(s) 82, 330
BspPI GGATC 1 cut(s) 501
BspT107I GGYRCC 1 cut(s) 328
BsrDI GCAATG 2 cut(s) 274, 374
BssECI CCNNGG 6 cut(s) 121, 201, 217, 218, 387, 871
BssMI GATC 2 cut(s) 493, 967
BssNI GRCGYC 1 cut(s) 329
BssT1I CCWWGG 2 cut(s) 201, 387
Bst2UI CCWGG 3 cut(s) 219, 326, 873
Bst4CI ACNGT 1 cut(s) 994
BstACI GRCGYC 1 cut(s) 329
BstBAI YACGTR 1 cut(s) 748
BstC8I GCNNGC 1 cut(s) 904
BstDEI CTNAG 2 cut(s) 354, 489
BstENI CCTNNNNNAGG 1 cut(s) 391
BstF5I GGATG 4 cut(s) 536, 678, 694, 931
BstFNI CGCG 2 cut(s) 23, 464
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 2 cut(s) 25, 331
BstKTI GATC 2 cut(s) 496, 970
BstMBI GATC 2 cut(s) 493, 967
BstMWI GCNNNNNNNGC 3 cut(s) 29, 132, 141
BstNI CCWGG 3 cut(s) 219, 326, 873
BstSCI CCNGG 3 cut(s) 217, 324, 871
BstSNI TACGTA 1 cut(s) 748
BstUI CGCG 2 cut(s) 23, 464
BstV1I GCAGC 1 cut(s) 1017
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BsuRI GGCC 2 cut(s) 81, 126
BtsCI GGATG 4 cut(s) 536, 678, 694, 931
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 904
CaiI CAGNNNCTG 1 cut(s) 563
CciI TCATGA 1 cut(s) 970
CfoI GCGC 2 cut(s) 25, 331
Cfr13I GGNCC 1 cut(s) 80
Csp6I GTAC 3 cut(s) 196, 251, 776
CspCI CAANNNNNGTGG 4 cut(s) 58, 93, 222, 257
CviAII CATG 7 cut(s) 58, 155, 254, 376, 706, 971, 1037
CviJI RGCY 9 cut(s) 81, 126, 144, 298, 351, 361, 527, 649, 906
CviKI_1 RGCY 9 cut(s) 81, 126, 144, 298, 351, 361, 527, 649, 906
CviQI GTAC 3 cut(s) 196, 251, 776
DdeI CTNAG 2 cut(s) 354, 489
DinI GGCGCC 1 cut(s) 330
DpnI GATC 2 cut(s) 495, 969
DpnII GATC 2 cut(s) 493, 967
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EciI GGCGGA 1 cut(s) 938
Ecl136II GAGCTC 1 cut(s) 361
Eco105I TACGTA 1 cut(s) 748
Eco130I CCWWGG 2 cut(s) 201, 387
Eco24I GRGCYC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 361
Eco88I CYCGRG 2 cut(s) 362, 953
EcoICRI GAGCTC 1 cut(s) 361
EcoNI CCTNNNNNAGG 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 1043
EcoRII CCWGG 3 cut(s) 217, 324, 871
EcoT14I CCWWGG 2 cut(s) 201, 387
EcoT38I GRGCYC 1 cut(s) 363
EgeI GGCGCC 1 cut(s) 330
EheI GGCGCC 1 cut(s) 330
ErhI CCWWGG 2 cut(s) 201, 387
FaeI CATG 7 cut(s) 61, 158, 257, 379, 709, 974, 1040
FatI CATG 7 cut(s) 57, 154, 253, 375, 705, 970, 1036
FauI CCCGC 1 cut(s) 758
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 94, 97, 1006
FokI GGATG 4 cut(s) 543, 685, 701, 938
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 3 cut(s) 94, 97, 1006
FspBI CTAG 1 cut(s) 497
GlaI GCGC 2 cut(s) 24, 330
GluI GCNGC 3 cut(s) 94, 97, 1006
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 2 cut(s) 81, 126
HhaI GCGC 2 cut(s) 25, 331
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 7 cut(s) 61, 158, 257, 379, 709, 974, 1040
Hin6I GCGC 2 cut(s) 23, 329
HinP1I GCGC 2 cut(s) 23, 329
HindIII AAGCTT 1 cut(s) 904
HinfI GANTC 3 cut(s) 213, 283, 425
HphI GGTGA 2 cut(s) 455, 860
Hpy166II GTNNAC 2 cut(s) 19, 814
Hpy188I TCNGA 2 cut(s) 912, 1049
Hpy188III TCNNGA 2 cut(s) 971, 1022
Hpy8I GTNNAC 2 cut(s) 19, 814
HpyCH4III ACNGT 1 cut(s) 994
HpyCH4IV ACGT 3 cut(s) 76, 290, 747
HpyCH4V TGCA 6 cut(s) 233, 379, 557, 610, 902, 1036
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 132, 141
HpyF3I CTNAG 2 cut(s) 354, 489
HpySE526I ACGT 3 cut(s) 76, 290, 747
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 7 cut(s) 61, 158, 257, 379, 709, 974, 1040
HspAI GCGC 2 cut(s) 23, 329
KasI GGCGCC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 493, 967
LmnI GCTCC 3 cut(s) 163, 358, 514
Lsp1109I GCAGC 1 cut(s) 1017
LweI GCATC 3 cut(s) 550, 951, 1017
MaeI CTAG 1 cut(s) 497
MaeII ACGT 3 cut(s) 76, 290, 747
MaeIII GTNAC 2 cut(s) 743, 929
MalI GATC 2 cut(s) 495, 969
MboI GATC 2 cut(s) 493, 967
MboII GAAGA 7 cut(s) 41, 257, 629, 644, 781, 851, 1040
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 2 cut(s) 363, 519
Mly113I GGCGCC 1 cut(s) 329
MmeI TCCRAC 1 cut(s) 897
MroXI GAANNNNTTC 2 cut(s) 454, 636
MseI TTAA 3 cut(s) 129, 303, 947
MslI CAYNNNNRTG 1 cut(s) 975
MspR9I CCNGG 3 cut(s) 219, 326, 873
Mva1269I GAATGC 2 cut(s) 235, 559
MvaI CCWGG 3 cut(s) 219, 326, 873
MvnI CGCG 2 cut(s) 23, 464
MwoI GCNNNNNNNGC 3 cut(s) 29, 132, 141
NarI GGCGCC 1 cut(s) 329
NdeII GATC 2 cut(s) 493, 967
NlaIII CATG 7 cut(s) 61, 158, 257, 379, 709, 974, 1040
NlaIV GGNNCC 2 cut(s) 82, 330
NmeAIII GCCGAG 1 cut(s) 102
PaeR7I CTCGAG 2 cut(s) 362, 953
PagI TCATGA 1 cut(s) 970
PasI CCCWGGG 1 cut(s) 218
PctI GAATGC 2 cut(s) 235, 559
PdmI GAANNNNTTC 2 cut(s) 454, 636
PfeI GAWTC 3 cut(s) 213, 283, 425
PkrI GCNGC 3 cut(s) 95, 98, 1007
PluTI GGCGCC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 748
PshBI ATTAAT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 3 cut(s) 217, 324, 871
PspGI CCWGG 3 cut(s) 217, 324, 871
PspN4I GGNNCC 2 cut(s) 82, 330
PspPI GGNCC 1 cut(s) 80
PspXI VCTCGAGB 2 cut(s) 362, 953
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 3 cut(s) 197, 252, 777
RsaNI GTAC 3 cut(s) 196, 251, 776
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 3 cut(s) 129, 303, 947
SatI GCNGC 3 cut(s) 94, 97, 1006
Sau3AI GATC 2 cut(s) 493, 967
Sau96I GGNCC 1 cut(s) 80
ScrFI CCNGG 3 cut(s) 219, 326, 873
SduI GDGCHC 2 cut(s) 363, 519
SfaNI GCATC 3 cut(s) 550, 951, 1017
SfoI GGCGCC 1 cut(s) 330
Sfr274I CTCGAG 2 cut(s) 362, 953
SlaI CTCGAG 2 cut(s) 362, 953
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 2 cut(s) 362, 953
SmoI CTYRAG 2 cut(s) 362, 953
SnaBI TACGTA 1 cut(s) 748
SsiI CCGC 9 cut(s) 36, 91, 94, 97, 133, 464, 670, 765, 923
SspDI GGCGCC 1 cut(s) 328
SspI AATATT 1 cut(s) 896
SspMI CTAG 1 cut(s) 497
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 3 cut(s) 217, 324, 871
StyI CCWWGG 2 cut(s) 201, 387
TaaI ACNGT 1 cut(s) 994
TaiI ACGT 3 cut(s) 79, 293, 750
TaqI TCGA 3 cut(s) 363, 954, 966
TatI WGTACW 1 cut(s) 250
TauI GCSGC 2 cut(s) 96, 99
TfiI GAWTC 3 cut(s) 213, 283, 425
Tru1I TTAA 3 cut(s) 129, 303, 947
Tru9I TTAA 3 cut(s) 129, 303, 947
TscAI CASTG 1 cut(s) 18
TseI GCWGC 1 cut(s) 1005
TspDTI ATGAA 9 cut(s) 242, 270, 334, 606, 645, 680, 694, 713, 1041
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 1 cut(s) 18
VspI ATTAAT 1 cut(s) 303
XagI CCTNNNNNAGG 1 cut(s) 391
XapI RAATTY 5 cut(s) 636, 700, 752, 794, 1043
XhoI CTCGAG 2 cut(s) 362, 953
XmiI GTMKAC 1 cut(s) 18
XmnI GAANNNNTTC 2 cut(s) 454, 636
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.