AT4G28080

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011973
Physical Location & Seq
Reverse (-)
13948603 .. 13958107
9505 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28080.1

Sequence Viewer

Length: 5460 bp
ATGGCTCCTAAAGCGGGGAAAACAAAGCCACACAAGAGTAAAGGAGAGAAGAAGAAGAAGGAAGAGAAAGTTTTGCCCACTGTCATTGAGATAAGTGTTGAAACACCAGATGAATCCCAAGTGACACTCAAGGGTATCTCCACAGACAGAATCTTAGATGTGAGGAAGCTCTTGGCGGTCCATGTTCAGACATGCCACTTCACCAACTTCTCTCTCTCTCACCAGGTGCGTGGCACTAAACTCAAGGATTCCGTTGACATCGTATCGCTCAAGCCCTGCCACCTCACTATTGTTGAAGAGGACTATACGGAGGAGCAAGCCACCGCGCACATACGGCGGCTCCTCGACATCGTCGCTTGCACCACCGCATTTGGTCCGTCGAAACCCCCGGTGTCTCGTACGCTTCCTAAGGATTCTGAAAAGAAGGAGTCAGGCTCGACGGATGGAGATTCTCCGACCGAAAAAGATGCCGGTGATTCAAATTCCGGTCTCAGCCCTAAACCTAAGGAATCAGAGAAGAAATCCGTCGGCGCATGTGAGGCTCAGTCGGCGGAAGGAGCGGCCAAGAGTGACATTGACATGTGCCCACCGACTCGTCTCGGACAATTTTACGAATTTTTCTCATTCTCCTACCTTACTCCTCCGATTCAATACATCAGAAGGTCTGTTCGTCCATCCAAAGAGGATAAAGGATTAGATGATTTGTTTCAAATCGATATTAAAGTTTCAAGTGGGAAGCCGTTCACGGTTGTTGCTTCAAGAACAGGTTTTTATCCACCTGGGAAACAGCAACTTCTGTGCCACTCCTTGGTTGAGCTGCTGCAACAGATAAGCCGGCCATTTGATGCGGCTTATGACGCTCTTATGAAAGCTTTCATTGAGCACAATAAGTTTGGCAACCTTCCTTATGGTTTCCGGGCAAACACTTGGGTAGTTCCTCCGGTTGTTGCAGATAGTCCATCTACTTTTCCGTCACTTCCAGTGGAGGATGAGACATGGGGAGGGGATGGTGGTGGGGTAGGCCGGTCTGGAAAGTATGACAAAAGAAAGTGGGCTAAGGAATTTGCCATTTTGGCAGCAATGCCTTGTAAAACACCTGAAGAGAGACAGGTTCGAGATAGGAAGGCCTTTCTACTCCACAGTTTATTTGTCGACGTTTCAGTTTTCAAAGCAGTGGAAATAATAAAAAAGATAGTAGAGAATAATCAATGCTCACTTAAGGATCCTGCTGCATTGGGTTTCCATGAAGAAAGAATAGGTGATTTGATCGTAAGGGTGGCTAGAGATGATCCTGACGCCAGTGCCAAGCTTGACCGGAAGAGCGATGGGACCCAGGTTCTGGAGATTTCTCAGGAAGAGCTTGCTCAAAGGAATTTGCTTAAAGGGATCACTGCTGATGAGAGTGCGACAGTTCATGATACGTCTACTTTGGGGGTGGTGGTTGTAAGACATTGTGGTTGTACAGCCATTGTAAAGGTTGCTTCTGAATTTAAGTTGAATGATGGCCATATCCTACAGGATATTGACATTGAAGACCAATCCGAAGGAGGTGCAAATGCACTGAATGTAAATAGCTTGAGAACTCTATTGCACAAGTCGTCAACGCCTTCTAGCTTAGCACAGAGATCACCAAATGCAGACTCGGAACAAATACGTGTGGCTAAGTCTCTTGTGAGGAAAGTAATCGAGGATAGTTTGAAGAAATTGGAGATTGAGCCCTCAAGATATAGTAAGCCGATAAGGTGGGAATTAGGAGCCTGTTGGGTGCAACATCTGCAAAATCAAGCTTCAAGTAAAAGTGAGTCTAAGAAAACTGAAGATCCCAAGCCTGAACCAGCTGTCAAGGGTCTTGGAAAGCAAGGTGCACTGCTGAAGGAGATAAAAAGGAAGATCGATGTGAAAGCTAACAAGACTGAACAGGGCAAAGAGGCCCCTGCTAATGACACTGACAATACATCAGAGACTGAGGATCAAAAGGAACTCGAGAAACAAAACGAGGAAATAGAAAAGATGTGGAAAGAACTGGTGACAGAAACTGCATATCAGCGCCTTAAAGAATCAGAAACTGGTTTCCATCTCAAGTCACCCAAAGAGCTGATTGAAATGGCCCGCAAATATTACACTGACACTGCTCTTCCAAAACTGGTGGCAGACTTTGGATCTCTTGAACTCTCACCCGTCGATGGAAGAACTCTGACAGACTTTATGCACACCAGAGGCTTGCAAATGCATTCCTTGGGAAGAGTGGTCGAGCTGGCTGAAAAGCTTCCTCATGTGCAATCCCTCTGTGTTCATGAAATGATTGTTCGGGCGTACAAGCATATACTGCAGGCTGTTGTAGCAGCTGTTGAAAACACCGCTGATGTGGCCACCTCAATAGCAACATGCTTAAATGTCCTATTGGGGACACCGTCTGACACTGAGAGTGTATACGATGAAAAGATTAAGTGGACCTGGGTGGAAACGTTCATTTCCAAGAGGTTTGGGTGGGATTGGAAGCATGAAGGCTGCCAGGAACTGAGAAAATTTTCCATTCTTAGAGGACTGTCACACAAGGTTGGACTGGAGCTTGTTCCTAAAGACTATGAGATGGACACGTCTTATCCTTTTAAGAAGTTTGATATTATCAGTATGGTTCCTGTGTATAAGCACGTAGCATGTTCATCCGCAGATGGGCGCACTTTGTTGGAATCATCCAAAACCTCCTTAGATAAAGGCAAACTGGAGGATGCTGTCAATTATGGCACCAAGGCATTGGCCAAGCTTGTAGCTGTGTGCGGCCCATATCATAGAATGACCGCTGGAGCGTATAGTCTTCTCGCTGTTGTGCTGTACCATACTGGGGATTTTAACCAGGCTACCATTTATCAGCAGAAAGCACTCGACATAAATGAAAGGGAACTTGGACTTGATCACCCAGACACAATGAAAAGCTACGGCGACCTGGCTGTCTTCTATTATCGTCTTCAGCACACAGAGTTAGCCTTGAAGTATGTCAACCGTGCCTTGTATCTTTTGCATCTAACATGTGGACCATCACATCCAAATACAGCTGCCACTTATATAAACGTAGCAATGATGGAAGAAGGTATGAAGAATGCCCATGTGGCTTTAAGATACCTTCATGAAGCATTGAAATGTAACCAGAGACTACTTGGAGCTGATCATATCCAGACTGCTGCAAGCTACCATGCCATTGCTATTGCTCTTTCTCTGATGGACGCATACTCCTTGAGCGTTCAACATGAGCAGACCACTCTACAGATTCTACAAGCAAAACTAGGCCCTGAGGATCTTCGGACACAGGATGCGGCTGCGTGGCTTGAATATTTCGAGTCCAAAGCTCTGGAACAGCAAGAAGCTGCACGAAATGGTACTCCCAAGCCAGACGCCTCTATTTCCAGCAAAGGCCATCTCAGTGTATCAGACCTGTTGGATTATATAACCCCGGATTCTGGTATTAAAGCAAGGGACGCTCAAAGGAAAGCTCGCCCAAAGGTCAAGGGAAAGCCAGGTCAAAGTCCAGGACCAGTGTCAGAAGAAAATCAGAAAGATGATGAAATTTTGAGTCCGGCTCATCTCACTGGGGAGAGTTCAAGTGATAAAGAGAACAAATCCGAAACAAAATCTGAAGAAAAAAAGGTTGAAAATTTTGATTTGGAACAATCAAAACCTCAAGACCAGCTGAAGCTGGTTAAGCCCGAAGCCACGGTCCACGAAGATGATGATTCTGACGAAGGATGGCAAGAAGCTGTCCCCAAAAATCGTTTTTCTTCTGGGCGTAGAACACGGCCCAGCCTGGCAAAGCTGAACACCAACTTCATGAACGTGACCCAGCAGCCATCAAGAAGCAGAGGAAAATCCACCAATTTCACATCCCCAAGAACCAGTTCTAATGAGCTTTCAATTTCTGTTGCTGGTTCCACTTCTTCCCCAGCCTCGAAGATGTTTGTAAAGAGCCCATTGAACAAAAAGCAAAACAATTCCAGTGTAGTGGGAGAAAGACCAGTCAACGATAAATCAGCCCTGGCAAGCTCAGCTTGCACTGAACAGATCAACAAGCCAACTCCGATGTTGAGTCCTGTGAGTGTCAAAGCTGGAAAACTGTTTTCCTATAAGGAGGTTGCACTGGCTCCCCCTGGAACGATTGTAAAAATAGTTGCAGAGCAGTTGCCAGAAGAAACAAAGGCCCCACAGAATTTGGATGCGGCAAAAATTGCAGTAGATGGTCCTGAAAAAGTTAATGCTCAGGATGCTGAGAGCGAGAATAAGCATGTGGCTACAGAAACGGAAGCGGAAAACACTGATTGCAATGAACAAGGAAGAGTTGTTGTTGGTGGATCGGAGTTGACTAGTTCTCCAAAGGAAATCAAGAATGTGGAGGTCGAAAAGGCAGCTGAAAAAGCATTTCCAATAGAAACAGCCGTATCAAATGCAAGACCAGGAAAATCTAAGAGTGCACAAATGGCTGAGGATTCAGATACATGTCTCTTGAATAAGTCACCAACAGCAAATGATTCAAATGGCAGTGAATCAGTTATTGGAGTGAAGCTTCAAAAGGATTTATGTGATGCTGAACTGAAAACAGTAGATGGTGAAACCGAAAATCTGCCCAATGGAGACTCAAGCCCAAAGTCATCAGTAGCTGCAGATGGAGAAAAGCAAGATGCATGTGAGGCACAAAAAGAGATGAGCAAGAAGCTCTCTGCTTCTGCACCGCCGTATACCCCAACAACCATTCCAATATTTGGATCAATTGCAGTTCCAGGATTCAAAGACCACGGTGGAATCCTCCCCTCTCCATTGAATATGCCACCAATGCTTCCTATTAACCATGTCCGCAGATCAACTCCTCATCAGTCTGTAACAGCTCGAGTTCCTTATGGGCCTAGACTCTCTGGTGGTGGTTACAACCGATCTGGAAACAGGGTTCCGCGTAACAAACCAAGCTTCCCCAATAGCACCGAGTCCAATGGTGAGGCTAATCAATTCAATGGCCCAAGAATAATGAACCCCCATGCTGCTGAGTTCATACCGAGTCAACCTTGGGTTTCTAATGGGTATCCAGTGTCACCAAATGGCTATTTAGCATCCCCAAATGGTGCAGAAATAACACAGAATGGGTACCCTTTGTCACCAGTAGCAGGTGGATATCCGTGTAACATGTCCGTTACACAGCCTCAGGATGGACTTGTTTCAGAGGAATTACCTGGTGCTGGAAGCTCTGAGGAGAAGAGCGGAAGCGAAGAAGAAAGCAACAACGACAAAAATGCTGGAGAGGATGACGAAGCCGTTGGACAAGAAACTACAGATACACCTGAAAATGGACATTCGACAGTAGGTGAAGTGGAAACCACATCACATGAGACTTGTGATGAGAAAAATGGAGAACGACAAGGAGGCAAGTGCTGGGGAGATTACAGCGATAATGAAATCGAGCAAATTGAAGTTACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1819

Amino Acids

199.11

Weight (kDa)

5.81

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 48 - 118 9.8e-11 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 724 - 863 1.5e-22 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 904 - 964 1.2e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 905 - 1003 4.8e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 933 - 1003 1.4e-12 Tetratricopeptide repeat
TPR_10 PF13374 933 - 974 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 1017 - 1091 7.4e-10 Tetratricopeptide repeat
TPR_10 PF13374 1017 - 1057 2.8e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5135
AasI GACNNNNNNGTC 1 cut(s) 2948
Acc36I ACCTGC 1 cut(s) 5135
Acc65I GGTACC 1 cut(s) 5124
AccB1I GGYRCC 2 cut(s) 2744, 5124
AccB7I CCANNNNNTGG 3 cut(s) 808, 1339, 4748
AccBSI CCGCTC 2 cut(s) 560, 5238
AccI GTMKAC 4 cut(s) 1152, 1424, 2430, 4724
AccII CGCG 2 cut(s) 326, 4936
AclI AACGTT 1 cut(s) 2465
AcoI YGGCCR 5 cut(s) 561, 836, 1504, 2367, 2757
AcsI RAATTY 9 cut(s) 481, 614, 1061, 1372, 1487, 2525, 3561, 3649, 4198
AcuI CTGAAG 6 cut(s) 1119, 1836, 1892, 2951, 3651, 3707
AcyI GRCGYC 2 cut(s) 1296, 3390
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 6 cut(s) 400, 1462, 2315, 2834, 3376, 5126
AflII CTTAAG 1 cut(s) 1217
AflIII ACRYGT 6 cut(s) 579, 1654, 2595, 3026, 4484, 5163
AhlI ACTAGT 1 cut(s) 4352
AjiI CACGTC 1 cut(s) 2598
AjuI GAANNNNNNNTTGG 6 cut(s) 2886, 2918, 3874, 3906, 4524, 4556
AloI GAACNNNNNNTCC 2 cut(s) 4914, 4946
Alw21I GWGCWC 3 cut(s) 885, 1867, 4462
Alw44I GTGCAC 2 cut(s) 1863, 4458
AlwNI CAGNNNCTG 6 cut(s) 1339, 1964, 2036, 2066, 2518, 4646
Ama87I CYCGRG 2 cut(s) 1982, 4872
ApaLI GTGCAC 2 cut(s) 1863, 4458
ApoI RAATTY 9 cut(s) 481, 614, 1061, 1372, 1487, 2525, 3561, 3649, 4198
ArsI GACNNNNNNTTYG 2 cut(s) 3696, 3728
Asp700I GAANNNNTTC 5 cut(s) 740, 872, 2265, 2527, 3889
Asp718I GGTACC 1 cut(s) 5124
AspLEI GCGC 4 cut(s) 328, 533, 2049, 2679
AsuC2I CCSGG 3 cut(s) 389, 917, 3449
AvaI CYCGRG 2 cut(s) 1982, 4872
AvaII GGWCC 8 cut(s) 178, 374, 1329, 2451, 3032, 3527, 3712, 4229
AxyI CCTNAGG 4 cut(s) 408, 504, 3288, 5181
BaeGI GKGCMC 3 cut(s) 587, 1867, 4462
BaeI ACNNNNGTAYC 2 cut(s) 4410, 4443
BalI TGGCCA 3 cut(s) 1506, 2369, 2759
BamHI GGATCC 1 cut(s) 1222
BanI GGYRCC 2 cut(s) 2744, 5124
BanII GRGCYC 2 cut(s) 1719, 3962
BbsI GAAGAC 4 cut(s) 1539, 2807, 2944, 2957
Bbv12I GWGCWC 3 cut(s) 885, 1867, 4462
BbvCI CCTCAGC 1 cut(s) 4470
BceAI ACGGC 7 cut(s) 350, 724, 2953, 3806, 4409, 4705, 5276
BcgI CGANNNNNNTGC 6 cut(s) 449, 483, 1532, 1566, 5252, 5286
BciVI GTATCC 1 cut(s) 5073
BclI TGATCA 2 cut(s) 2911, 3163
BcnI CCSGG 3 cut(s) 389, 917, 3449
BcuI ACTAGT 1 cut(s) 4352
BfaI CTAG 5 cut(s) 1281, 1611, 3281, 4353, 4890
BfmI CTRYAG 6 cut(s) 1514, 2327, 3260, 4281, 4647, 5307
BfoI RGCGCY 1 cut(s) 2050
BfrI CTTAAG 1 cut(s) 1217
BfuAI ACCTGC 1 cut(s) 5135
BfuI GTATCC 1 cut(s) 5073
BglI GCCNNNNNGGC 2 cut(s) 1073, 3107
BlpI GCTNAGC 2 cut(s) 1615, 4036
Bme18I GGWCC 8 cut(s) 178, 374, 1329, 2451, 3032, 3527, 3712, 4229
BmeT110I CYCGRG 2 cut(s) 1982, 4872
BmgBI CACGTC 1 cut(s) 2598
BmrI ACTGGG 2 cut(s) 2850, 3594
BmuI ACTGGG 2 cut(s) 2850, 3594
BoxI GACNNNNGTC 1 cut(s) 3532
BpiI GAAGAC 4 cut(s) 1539, 2807, 2944, 2957
BplI GAGNNNNNCTC 4 cut(s) 419, 451, 3559, 3591
BpmI CTGGAG 5 cut(s) 1361, 2585, 2744, 2823, 5295
Bpu10I CCTNAGC 3 cut(s) 1056, 4248, 4470
Bpu1102I GCTNAGC 2 cut(s) 1615, 4036
BpuEI CTTGAG 9 cut(s) 113, 227, 254, 1597, 1705, 2063, 3253, 3660, 4609
BpuMI CCSGG 3 cut(s) 389, 917, 3449
Bsa29I ATCGAT 2 cut(s) 714, 1893
BsaAI YACGTR 2 cut(s) 1655, 2653
BsaHI GRCGYC 2 cut(s) 1296, 3390
BsaI GGTCTC 1 cut(s) 494
BsaWI WCCGGW 3 cut(s) 485, 940, 1314
BsaXI ACNNNNNCTCC 6 cut(s) 324, 354, 3212, 3242, 4342, 4372
Bse118I RCCGGY 3 cut(s) 470, 834, 1023
Bse21I CCTNAGG 4 cut(s) 408, 504, 3288, 5181
Bse3DI GCAATG 4 cut(s) 1086, 3081, 3195, 4318
BseCI ATCGAT 2 cut(s) 714, 1893
BseMI GCAATG 4 cut(s) 1086, 3081, 3195, 4318
BseRI GAGGAG 5 cut(s) 326, 332, 630, 4842, 5243
BseSI GKGCMC 3 cut(s) 587, 1867, 4462
BseYI CCCAGC 4 cut(s) 3794, 3834, 3934, 5409
BsgI GTGCAG 3 cut(s) 3348, 4698, 5124
Bsh1236I CGCG 2 cut(s) 326, 4936
Bsh1285I CGRYCG 1 cut(s) 459
BshNI GGYRCC 2 cut(s) 2744, 5124
BshVI ATCGAT 2 cut(s) 714, 1893
BsiEI CGRYCG 1 cut(s) 459
BsiHKAI GWGCWC 3 cut(s) 885, 1867, 4462
BsiHKCI CYCGRG 2 cut(s) 1982, 4872
BsiWI CGTACG 1 cut(s) 398
BslFI GGGAC 4 cut(s) 1342, 2419, 3485, 3740
BsmBI CGTCTC 1 cut(s) 602
BsmFI GGGAC 4 cut(s) 1342, 2419, 3485, 3740
BsmI GAATGC 2 cut(s) 2230, 3103
Bso31I GGTCTC 1 cut(s) 494
BsoBI CYCGRG 2 cut(s) 1982, 4872
Bsp1286I GDGCHC 6 cut(s) 587, 885, 1719, 1867, 3962, 4462
Bsp1407I TGTACA 1 cut(s) 1460
Bsp1720I GCTNAGC 2 cut(s) 1615, 4036
BspDI ATCGAT 2 cut(s) 714, 1893
BspFNI CGCG 2 cut(s) 326, 4936
BspHI TCATGA 4 cut(s) 1414, 2293, 3124, 3822
BspMAI CTGCAG 2 cut(s) 2331, 4651
BspMI ACCTGC 1 cut(s) 5135
BspQI GCTCTTC 4 cut(s) 1313, 1350, 2139, 5228
BspT107I GGYRCC 2 cut(s) 2744, 5124
BspTI CTTAAG 1 cut(s) 1217
BspTNI GGTCTC 1 cut(s) 494
BsrBI CCGCTC 2 cut(s) 560, 5238
BsrDI GCAATG 4 cut(s) 1086, 3081, 3195, 4318
BsrFI RCCGGY 3 cut(s) 470, 834, 1023
BsrGI TGTACA 1 cut(s) 1460
BssAI RCCGGY 3 cut(s) 470, 834, 1023
BssNAI GTATAC 2 cut(s) 2431, 4725
BssNI GRCGYC 2 cut(s) 1296, 3390
BssT1I CCWWGG 4 cut(s) 807, 2235, 2748, 5045
Bst1107I GTATAC 2 cut(s) 2431, 4725
Bst6I CTCTTC 9 cut(s) 57, 291, 1095, 1313, 1350, 2139, 2236, 4318, 5228
BstACI GRCGYC 2 cut(s) 1296, 3390
BstAFI CTTAAG 1 cut(s) 1217
BstAPI GCANNNNNTGC 3 cut(s) 1774, 2326, 4217
BstAUI TGTACA 1 cut(s) 1460
BstBAI YACGTR 2 cut(s) 1655, 2653
BstDSI CCRYGG 2 cut(s) 3708, 4780
BstFNI CGCG 2 cut(s) 326, 4936
BstH2I RGCGCY 1 cut(s) 2050
BstHHI GCGC 4 cut(s) 328, 533, 2049, 2679
BstMCI CGRYCG 1 cut(s) 459
BstPAI GACNNNNGTC 1 cut(s) 3532
BstSFI CTRYAG 6 cut(s) 1514, 2327, 3260, 4281, 4647, 5307
BstSLI GKGCMC 3 cut(s) 587, 1867, 4462
BstUI CGCG 2 cut(s) 326, 4936
BstV2I GAAGAC 4 cut(s) 1539, 2807, 2944, 2957
BstX2I RGATCY 4 cut(s) 1222, 1819, 2159, 3292
BstXI CCANNNNNNTGG 4 cut(s) 230, 2755, 3346, 3994
BstYI RGATCY 4 cut(s) 1222, 1819, 2159, 3292
BstZ17I GTATAC 2 cut(s) 2431, 4725
Bsu15I ATCGAT 2 cut(s) 714, 1893
Bsu36I CCTNAGG 4 cut(s) 408, 504, 3288, 5181
BsuI GTATCC 1 cut(s) 5073
BsuTUI ATCGAT 2 cut(s) 714, 1893
BtgI CCRYGG 2 cut(s) 3708, 4780
BtgZI GCGATG 1 cut(s) 1338
BtrI CACGTC 1 cut(s) 2598
BtsI GCAGTG 5 cut(s) 1179, 1389, 1865, 2127, 4534
BveI ACCTGC 1 cut(s) 5135
CaiI CAGNNNCTG 6 cut(s) 1339, 1964, 2036, 2066, 2518, 4646
CciI TCATGA 4 cut(s) 1414, 2293, 3124, 3822
CfoI GCGC 4 cut(s) 328, 533, 2049, 2679
Cfr10I RCCGGY 3 cut(s) 470, 834, 1023
ClaI ATCGAT 2 cut(s) 714, 1893
CseI GACGC 5 cut(s) 866, 1304, 3230, 3398, 3482
CsiI ACCWGGT 2 cut(s) 222, 5209
Csp6I GTAC 6 cut(s) 399, 1461, 2314, 2833, 3375, 5125
CspCI CAANNNNNGTGG 8 cut(s) 67, 102, 352, 387, 2127, 2162, 4182, 4217
CviQI GTAC 6 cut(s) 399, 1461, 2314, 2833, 3375, 5125
DraIII CACNNNGTG 1 cut(s) 226
DrdI GACNNNNNNGTC 1 cut(s) 2948
DseDI GACNNNNNNGTC 1 cut(s) 2948
EaeI YGGCCR 5 cut(s) 561, 836, 1504, 2367, 2757
Eam1104I CTCTTC 9 cut(s) 57, 291, 1095, 1313, 1350, 2139, 2236, 4318, 5228
EarI CTCTTC 9 cut(s) 57, 291, 1095, 1313, 1350, 2139, 2236, 4318, 5228
EciI GGCGGA 1 cut(s) 566
Eco130I CCWWGG 4 cut(s) 807, 2235, 2748, 5045
Eco147I AGGCCT 1 cut(s) 1127
Eco24I GRGCYC 2 cut(s) 1719, 3962
Eco31I GGTCTC 1 cut(s) 494
Eco32I GATATC 1 cut(s) 5153
Eco47I GGWCC 8 cut(s) 178, 374, 1329, 2451, 3032, 3527, 3712, 4229
Eco57I CTGAAG 6 cut(s) 1119, 1836, 1892, 2951, 3651, 3707
Eco81I CCTNAGG 4 cut(s) 408, 504, 3288, 5181
Eco88I CYCGRG 2 cut(s) 1982, 4872
EcoO109I RGGNCCY 4 cut(s) 1329, 1930, 3284, 4189
EcoRV GATATC 1 cut(s) 5153
EcoT14I CCWWGG 4 cut(s) 807, 2235, 2748, 5045
EcoT22I ATGCAT 2 cut(s) 2232, 4672
EcoT38I GRGCYC 2 cut(s) 1719, 3962
ErhI CCWWGG 4 cut(s) 807, 2235, 2748, 5045
Esp3I CGTCTC 1 cut(s) 602
FalI AAGNNNNNCTT 2 cut(s) 4024, 4056
FaqI GGGAC 4 cut(s) 1342, 2419, 3485, 3740
FauI CCCGC 2 cut(s) 7, 2117
FbaI TGATCA 2 cut(s) 2911, 3163
FblI GTMKAC 4 cut(s) 1152, 1424, 2430, 4724
FriOI GRGCYC 2 cut(s) 1719, 3962
FspBI CTAG 5 cut(s) 1281, 1611, 3281, 4353, 4890
GlaI GCGC 4 cut(s) 327, 532, 2048, 2678
GsaI CCCAGC 4 cut(s) 3798, 3838, 3938, 5413
GsuI CTGGAG 5 cut(s) 1361, 2585, 2744, 2823, 5295
HaeII RGCGCY 1 cut(s) 2050
HgaI GACGC 5 cut(s) 866, 1304, 3230, 3398, 3482
HhaI GCGC 4 cut(s) 328, 533, 2049, 2679
Hin1I GRCGYC 2 cut(s) 1296, 3390
Hin6I GCGC 4 cut(s) 326, 531, 2047, 2677
HinP1I GCGC 4 cut(s) 326, 531, 2047, 2677
HincII GTYRAC 7 cut(s) 256, 1153, 1602, 2998, 4012, 4350, 5042
HindII GTYRAC 7 cut(s) 256, 1153, 1602, 2998, 4012, 4350, 5042
HindIII AAGCTT 7 cut(s) 870, 1307, 1785, 2264, 2762, 4550, 4948
Hpy99I CGWCG 6 cut(s) 356, 382, 442, 530, 1157, 2183
HpyCH4IV ACGT 8 cut(s) 1155, 1421, 1654, 2465, 2597, 2652, 3069, 3828
HpySE526I ACGT 8 cut(s) 1155, 1421, 1654, 2465, 2597, 2652, 3069, 3828
Hsp92I GRCGYC 2 cut(s) 1296, 3390
HspAI GCGC 4 cut(s) 326, 531, 2047, 2677
KflI GGGWCCC 1 cut(s) 1329
KpnI GGTACC 1 cut(s) 5128
KroI GCCGGC 1 cut(s) 834
KroNI GCCGGC 1 cut(s) 836
Ksp22I TGATCA 2 cut(s) 2911, 3163
LguI GCTCTTC 4 cut(s) 1313, 1350, 2139, 5228
LmnI GCTCC 9 cut(s) 10, 313, 345, 557, 1754, 2566, 2804, 3158, 4138
MabI ACCWGGT 2 cut(s) 222, 5209
MaeI CTAG 5 cut(s) 1281, 1611, 3281, 4353, 4890
MaeII ACGT 8 cut(s) 1155, 1421, 1654, 2465, 2597, 2652, 3069, 3828
MbiI CCGCTC 2 cut(s) 560, 5238
MfeI CAATTG 1 cut(s) 4755
MflI RGATCY 4 cut(s) 1222, 1819, 2159, 3292
MhlI GDGCHC 6 cut(s) 587, 885, 1719, 1867, 3962, 4462
MlsI TGGCCA 3 cut(s) 1506, 2369, 2759
MluNI TGGCCA 3 cut(s) 1506, 2369, 2759
MmeI TCCRAC 5 cut(s) 479, 2539, 2667, 3414, 5275
Mox20I TGGCCA 3 cut(s) 1506, 2369, 2759
Mph1103I ATGCAT 2 cut(s) 2232, 4672
MroNI GCCGGC 1 cut(s) 834
MroXI GAANNNNTTC 5 cut(s) 740, 872, 2265, 2527, 3889
MscI TGGCCA 3 cut(s) 1506, 2369, 2759
MslI CAYNNNNRTG 5 cut(s) 578, 3136, 3417, 3720, 3827
Msp20I TGGCCA 3 cut(s) 1506, 2369, 2759
MspA1I CMGCKG 7 cut(s) 1838, 2345, 2360, 2801, 3053, 3685, 4397
MspCI CTTAAG 1 cut(s) 1217
MunI CAATTG 1 cut(s) 4755
Mva1269I GAATGC 2 cut(s) 2230, 3103
MvnI CGCG 2 cut(s) 326, 4936
NaeI GCCGGC 1 cut(s) 836
NciI CCSGG 3 cut(s) 389, 917, 3449
NgoMIV GCCGGC 1 cut(s) 834
NsiI ATGCAT 2 cut(s) 2232, 4672
PaeR7I CTCGAG 2 cut(s) 1982, 4872
PagI TCATGA 4 cut(s) 1414, 2293, 3124, 3822
PaqCI CACCTGC 1 cut(s) 5135
PceI AGGCCT 1 cut(s) 1127
PciI ACATGT 4 cut(s) 579, 3026, 4484, 5163
PciSI GCTCTTC 4 cut(s) 1313, 1350, 2139, 5228
PctI GAATGC 2 cut(s) 2230, 3103
PdiI GCCGGC 1 cut(s) 836
PdmI GAANNNNTTC 5 cut(s) 740, 872, 2265, 2527, 3889
Pfl23II CGTACG 1 cut(s) 398
PflFI GACNNNGTC 2 cut(s) 350, 2410
PflMI CCANNNNNTGG 3 cut(s) 808, 1339, 4748
PfoI TCCNGGA 2 cut(s) 3523, 4765
Ppu21I YACGTR 2 cut(s) 1655, 2653
PpuMI RGGWCCY 1 cut(s) 1329
PscI ACATGT 4 cut(s) 579, 3026, 4484, 5163
PshAI GACNNNNGTC 1 cut(s) 3532
Psp1406I AACGTT 1 cut(s) 2465
Psp5II RGGWCCY 1 cut(s) 1329
PspFI CCCAGC 4 cut(s) 3794, 3834, 3934, 5409
PspLI CGTACG 1 cut(s) 398
PspPPI RGGWCCY 1 cut(s) 1329
PspXI VCTCGAGB 1 cut(s) 4872
PstI CTGCAG 2 cut(s) 2331, 4651
PstNI CAGNNNCTG 6 cut(s) 1339, 1964, 2036, 2066, 2518, 4646
PsuI RGATCY 4 cut(s) 1222, 1819, 2159, 3292
PsyI GACNNNGTC 2 cut(s) 350, 2410
PvuII CAGCTG 5 cut(s) 1838, 2345, 3053, 3685, 4397
RsaI GTAC 6 cut(s) 400, 1462, 2315, 2834, 3376, 5126
RsaNI GTAC 6 cut(s) 399, 1461, 2314, 2833, 3375, 5125
RseI CAYNNNNRTG 5 cut(s) 578, 3136, 3417, 3720, 3827
SalI GTCGAC 1 cut(s) 1151
SapI GCTCTTC 4 cut(s) 1313, 1350, 2139, 5228
SduI GDGCHC 6 cut(s) 587, 885, 1719, 1867, 3962, 4462
SexAI ACCWGGT 2 cut(s) 222, 5209
SfcI CTRYAG 6 cut(s) 1514, 2327, 3260, 4281, 4647, 5307
Sfr274I CTCGAG 2 cut(s) 1982, 4872
SinI GGWCC 8 cut(s) 178, 374, 1329, 2451, 3032, 3527, 3712, 4229
SlaI CTCGAG 2 cut(s) 1982, 4872
SmiMI CAYNNNNRTG 5 cut(s) 578, 3136, 3417, 3720, 3827
SpeI ACTAGT 1 cut(s) 4352
SseBI AGGCCT 1 cut(s) 1127
SspI AATATT 3 cut(s) 2117, 3329, 4746
SspMI CTAG 5 cut(s) 1281, 1611, 3281, 4353, 4890
StuI AGGCCT 1 cut(s) 1127
StyI CCWWGG 4 cut(s) 807, 2235, 2748, 5045
TaiI ACGT 8 cut(s) 1158, 1424, 1657, 2468, 2600, 2655, 3072, 3831
TaqII GACCGA 1 cut(s) 473
TatI WGTACW 1 cut(s) 1460
TauI GCSGC 6 cut(s) 340, 563, 851, 2781, 3314, 4211
TspGWI ACGGA 9 cut(s) 241, 323, 366, 455, 514, 960, 4304, 5145, 5158
Tth111I GACNNNGTC 2 cut(s) 350, 2410
Van91I CCANNNNNTGG 3 cut(s) 808, 1339, 4748
Vha464I CTTAAG 1 cut(s) 1217
VneI GTGCAC 2 cut(s) 1863, 4458
VpaK11BI GGWCC 8 cut(s) 178, 374, 1329, 2451, 3032, 3527, 3712, 4229
XapI RAATTY 9 cut(s) 481, 614, 1061, 1372, 1487, 2525, 3561, 3649, 4198
XcmI CCANNNNNNNNNTGG 1 cut(s) 3152
XhoI CTCGAG 2 cut(s) 1982, 4872
XmiI GTMKAC 4 cut(s) 1152, 1424, 2430, 4724
XmnI GAANNNNTTC 5 cut(s) 740, 872, 2265, 2527, 3889
XspI CTAG 5 cut(s) 1281, 1611, 3281, 4353, 4890
Zsp2I ATGCAT 2 cut(s) 2232, 4672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.