FvH4_3g24640

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011973
Physical Location & Seq
Reverse (-)
17724630 .. 17739664
15035 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24640.t3

Sequence Viewer

Length: 5556 bp
ATGGCTCCCAAAACAGGGAAAGCCAAGCCACACAAATCCAAGGGAGACAAGAAGAAGAAAGAAGAGAAAGTTTTGCCTACCGTAATCGAGATTAGCATTGAAACACCGGATGAATCACAAGTGACCCTCAAGGGTATCTCCACGGACAGGATCCTAGACGTAAGAAAGCTGCTGGCAGTCCACGTGGATACATGTCATTTGACCAATTTCTCCCTATCACACGAGGTGCGAGGACCCAGACTGAAAGATACTGTGGACATCCTCTCTCTCAAGCCCTGCCACCTCACCATCGTCGAAGAGGACTACACGGAAGAGCAAGCCGTCGCTCACATCCGACGACTTGTCGACATCGTCGCCTGCACGACGTCGTTTGGGTCGTCGTCGTCCTCTTCGCCCAGAACTCCCGGTTCCGCTCCCGTTCCGGCTCCGGTTGGGTCTAATTCCAAAGATTCCGGTTTAGACGAGGGCGACCAAAACGGCGACGAGCACAATGCCGTTCAGAAGACCAAAGTCTCGAGTCCGATTCCGGTCGCCGGCGATAAAGGCGGCGAGTCGGCAATGTATCCTCCGCCGCGGCTCGGCCAGTTCTACGACTTCTTCTCCTTGGCTCACCTCACTCCTCCGCTCCACTACGTCAGAAGATCTAGCCGTCCGTTCCTTGAGGACAAGACGGAGGAGGACCTGTTTCAAATTGATGTTAGAGTTTGTAGTGGGAAGCCGACGACTATAGTTGCATCCAGAAAAGGATTCTACCCTGCTGGAAAACGTCCTCTAATTACTCACTCTTTGGTTGCTTTGCTTCAACAGATAAGCCGGCCTTTCGATGCCGCATACAATGCTGTCATGAAGGCTTTCACCGAGCACAATAAATTTGGAAACCTTCCTTACGGTTTTCGGGCAAACACATGGGTTGTCCCTCCAGTTGTTGCCGAGAATCCGTCTGTTTTTCCGCCGCTTCCTCTAGAAGATGAAAGTTGGGGAGGAAGTGGGGGAGGGCAGGGAAGAGACGGTAAACATGACAACAGGCCGTGGGGAAAAGAGTTTGCCATTCTGGCAGCAATGCCTTGTGCAACAGCAGAGGAGAGGCAAATTCGAGATCGAAAGGCCTTCCTGCTTCACAGTTTATTTGTCGACGTTTCAGTTCTTAAAGCTGTTGCAGCTATTAAGAGCCTAATCGACACTCATCAATCTTCCCTAAATGATTCTACTCTTTCACTTCATCATGAGGTAAAAGTCGGAGATTTAAGCATTAAGATTGTTAGAGATGCACCTGATGCAAGCATAAAGGTGGACTGTAAGAATGATGGCAGTCAAGTTCTAGGAATTCCACAAGAAGAAGTTACTCAGAGAAACCTACTCAAAGGCATAACAGCTGATGAGAGTGCAACTGTTCATGATACTTCTACGCTGGGTGTCGTGGTTGTTAGGCATTGTGGATTTACCGCTGTTGTGAAAGTTTTATCTGAGGTGAATTGGGTGGGAAGACCTGTTCCTCAGGACATTGAAATTGAGGACCAGCCTGAAGGAGGTGCAAATGCCTTGAATGTTAACAGCCTGAGAATGCTATTGCAACAGTCATCCCTCCTTCAGTCAACCACAGTTCAGCGGAGCCAGAGTACAGATCTTGAAAGTTTACATTCTGCTAGGTCCTTAGTAAGGAAGGTACTCGAAGAAAGTTTGTTGAGGTTGCAGGGTGGACCATCTAATCACACCAAGTCAATCAGATGGGAGCTAGGAGCATGTTGGGTGCAACATTTGCAAAATCAGGCTTCAGCGAAAAATGAGCCTAAGAAAAATGAAGAAGCTAAGATTGAGCTGGCTGTTAAGGGCCTTGGAAAGCAAGGTGGACTGCTAAAGGAAATAAAGAAGAAAATGGATATAAGGAGCAGTAAAACTGAACAGGGGAAGGAAGTTCTTGGGGGTAACAATCTTGATCATAATAAGAATTCAGACACCAGCAGTCAGGAGGAGTTACAAAAGCGAGATGCAGAAAACAAAATAAATTGGAGAAAGCTACTTCCTGATGCATCATATTCACGCCTCAAAGAATCAGATACTGGTCTTCACCTTAAGTCACCCGATGAGTTGATTGAAATGGCACACAAGTATTATGCTGACACTGCACTTCCAAAACTGGTGGCAGATTTCGGCTCCCTTGAACTTTCACCAGTTGATGGAAGGACATTGACAGATTTTATGCATACAAGGGGTTTGAAAATGTGTTCCCTGGGACGCGTGGTTGAACTTGCAGATAAGCTCCCTCATGTGCAATCACTCTGTATACATGAGATGGTTGTGCGAGCATACAAACACATACTGCAAGCTGTTGTAGCAGCAGTTGATAACGTTGCAGACTTGGCTGCATCAATAGCAGCATGTCTAAATATTCTTCTTGGAACCCCATCAGCTGAAAATGGGGATGGAGCTTGTGATGATATGTTAAAATGGAAGTGGGTGGAAACATTTCTTTTGAAGAGGTTTGGATGGCAATGGAAACATGAAAGTGTTGAGGACTTGAGAAAGTTTGCCATTCTTCGTGGATTGTGCCATAAGGTTGGCCTTGAGCTTGTGCCAAGGGACTACGATATGGACACTTCGTCTCCTTTTAGGAAATCAGATATAGTAAGCATGGTCCCTGTGTACAAGCATGTTGCCTGTTCCTCTGCTGATGGACGCACACTTTTGGAATCTTCTAAGACTTCTTTGGACAAAGGTAAACTGGAGGATGCTGTTAATTATGGCACTAAGGCGTTGTCAAAACTTGTATCAGTATGTGGGCCTTATCATCGAATGACTGCTGGAGCATATAGTCTTCTTGCTGTAGTACTGTATCACACTGGGGATTTTAATCAGGCAACCATCTACCAGCAAAAAGCATTGGATATTAATGAGAGAGAGCTTGGACTTGATCATCCTGATACAATGAAAAGTTACGGGGATTTAGCTGTGTTCTACTACCGACTTCAACACACAGAGTTGGCCTTGAAGTATGTCAATCGTGCGCTGTATCTTTTGCACCTGACATGTGGGCCATCTCATCCAAATACGGCTGCCACCTATATCAACGTAGCAATGATGGAAGAAGGTTTGGGGAACGTCCATGTTGCTCTTAGGTACCTGCATGAAGCTCTCAAGTGCAATCAAAGGCTACTTGGAGCTGATCATATACAGACTGCTGCTAGCTATCATGCCATAGCTATCGCACTTTCATTGATGGAGGCCTACTCGTTAAGTGTTCAGCACGAACAGACTACCTTGCAAATTCTTCAGGCAAAACTTGGGTCTGAGGATCTACGCACACAGGATGCGGCTGCATGGCTTGAATATTTTGAGTCCAAAGCTTTGGAGCAGCAAGAAGCTGCACGGAATGGTACTCCAAAGCCAGATGCCTCAATCTCCAGCAAAGGTCATTTGAGTGTCTCAGATCTTTTGGATTATATAACACCAGATGCTGATATGAAAGCAAGAGAGGCCCAAAGGAAAGCTCGTTTGAAGGTAAAAGGAAAACCAGGTCAGAATGGGGAGGCTGTCTCTGATGAATACCAGAAGGATGAAAATTTGTTACCAAGTCATCCTGTTGCAGAGAATTTAAGTGATAAAGAAAACAAGTCTGAAGCACATGTAGCAGAACCTAGGAATGAGAAATCTGATTCACGACTATTTGAGCAATCAATTAATTTTGCTACAAGTGATGATCTGGCACAAGATGACACCTCAGATGAAGGCTGGCAAGAGGCTGTTCCTAAAGGCCGGTCACTTATAGGTCGCAAATCTCCTGGGTCGAGGAGGCCAAGCCTTGAAAAACTGAATACGAACTTTATCAATGCATCCCAGCCAGCAAGATATCGAGGCAAGGCCAATAATTTCACATCCCCAAAATCAAGCCCAAATGAGCCTGCTTCTTCAACTGGACCTGGTCTTCCAGTGTCAAAGAAGTTTGTCAAGAGTGCAAGCTTCAGTCCTAAGCCAAACAACTCTAGCACATCAGCTGCTGGAGCTGCTGATAGATTGCCAAACCCAAAGTCAGCACCATCTACCCCAGCTTCTGTTGATCAAGTTGTGAAGTCTGTAAGTTCAATCAGTGTTCAGTCAGCTGGAAAACTCTTTTCCTACAAGGAAGTTGCTTTGGCTCCACCTGGGACAATTGTGAAGGCAGTGGCAGAGCAGTTACCGAAGGGAAATCTTCCTATTGTACAAACTTCTCAGGTGGGCCTGGAGACACCTACAACAGAGGTCACTGTTGGTGAAGTGACAGCAATAAAAGATATGAAGGAAGACAAAAATCAGAAACCCACAGGAGAGAAAGAGATTGTGGTTCCTGAGAAAACCCCTTTTGTTAAACGAGCAGCAAAAGTGAAGGACTCTGTGGCAAAGGAGTCCCTAGAAGTTGTAAAGCATGCTAGTGTTGGAGTACAAGTTGAAGCTGAAGCTGTAGAATTAGAAAACCCTGCTTTTGAAGGCTCAGCATTACAGACTGTTAAAGTTCCTGTTCCTGGGGTTGAGATTGCTGATACTTCCCAAGGTCCAAATACCACTGCTTCTGAATGTGGGCTGTCAGAAGTCCTAGGGCCAGACAGTTGCCTGAGGACTTCCTCTGTTTCAGAACCTCCATCTGGTTTAACAGAAACTGGAACCGATAATCCTTCTAATACAGAGGAGGGAAAGTCACGTGATTTACCCAGTGGCGACGTAGTTAAACCAGTGCCAACTGATGGGGAAAAGGTAGATGAACAAGAAACTGGAAAGGAGACGAGCAAAAAACTGTCTGCAGCTGCACCTCCTTATAATCCATCACTCATTCCAGTTTTTGGCTCTATTCCGGTTCCAGTTCCAGGTTTCAAAGATCATGGTGGGATTTTGCCCCCACCTGTTAATATTCCTCCAATGCTAGCAGTTAATCCTGTCCGCAGGTCACCTCATCAGTCAGCAACAGCAAGAGTTCCTTATGGTCCACGACTGTCTGGTGGCTATAACAGATCTGGAAGTCGGGTTTCACACAATAAACATAGCTTTCAAAATGGCGAGCATACTGGGGATGGTCCTCCTAGAATTATGAACCCACATGCAGCTGAGTTCGTACCTGGCCAACCTTGGGTTCAAAACGGCTATCCAGTGTCTCCCAATGGTTTCTTGCCTTCCCCAAATGGTTATCCAGTGTCTCCGAATGGATATCCGGTATCACCGAATGGTACTCCGGTGATTCAAAACGGATCTCCGACATCTCCTGTCAGTTCAGACGAGTCATCACCAGTGGTGTCTGCTGATATTGGTGTTGGAGCAAGCACTGAAGGGGCAGCAAAAGAGACTGATGATAAGTTGTCAGTACAAGTTGAATGTGACAAGGAACCAATTGAAGGAAAGCTGCAGGAAGAGCAATCTGTAGATAATGTAAATGTTTGTCCTGAATTTGAAGAGAAGCCTATTGATACCGATACTGTTCCTGGTGATACTAGTGTGGAAAAGGAGGCTAGCAATTTAGTAGTTGAGGAAAAACCAAGCAAATGTTGGGGAGATTATAGTGACAATGAAGCTGAGGTCATTGAGATTAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1852

Amino Acids

200.67

Weight (kDa)

5.95

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_p135 PF12807 535 - 676 1.3e-22 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 717 - 777 2.6e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 717 - 816 2.5e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 746 - 816 1.3e-12 Tetratricopeptide repeat
TPR_10 PF13374 746 - 787 1.9e-09 Tetratricopeptide repeat
TPR_12 PF13424 830 - 904 1.8e-10 Tetratricopeptide repeat
TPR_10 PF13374 830 - 870 3.7e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4785
AatII GACGTC 1 cut(s) 368
Acc36I ACCTGC 2 cut(s) 3126, 4899
Acc65I GGTACC 1 cut(s) 3114
AccB1I GGYRCC 1 cut(s) 3114
AccB7I CCANNNNNTGG 3 cut(s) 2174, 4712, 4808
AccBSI CCGCTC 2 cut(s) 413, 625
AccI GTMKAC 3 cut(s) 345, 1131, 2281
AccII CGCG 2 cut(s) 574, 2235
AclI AACGTT 1 cut(s) 2346
AclWI GGATC 4 cut(s) 145, 158, 3297, 5218
AcoI YGGCCR 2 cut(s) 580, 5083
AcsI RAATTY 8 cut(s) 869, 1089, 1323, 1945, 3261, 3556, 3586, 5405
AcuI CTGAAG 8 cut(s) 1542, 1571, 1755, 3251, 3633, 3940, 4446, 5307
AcvI CACGTG 2 cut(s) 184, 4670
AcyI GRCGYC 1 cut(s) 365
AdeI CACNNNGTG 1 cut(s) 226
AflII CTTAAG 1 cut(s) 2069
AflIII ACRYGT 4 cut(s) 191, 2233, 3023, 3619
AhdI GACNNNNNGTC 2 cut(s) 341, 2596
AhlI ACTAGT 1 cut(s) 5450
AjuI GAANNNNNNNTTGG 2 cut(s) 5079, 5111
AloI GAACNNNNNNTCC 4 cut(s) 1473, 1505, 4624, 4656
Alw21I GWGCWC 2 cut(s) 489, 864
AlwI GGATC 4 cut(s) 145, 158, 3297, 5218
AlwNI CAGNNNCTG 5 cut(s) 2057, 3452, 3992, 4046, 4628
Ama87I CYCGRG 1 cut(s) 514
ApoI RAATTY 8 cut(s) 869, 1089, 1323, 1945, 3261, 3556, 3586, 5405
ArsI GACNNNNNNTTYG 4 cut(s) 3266, 3298, 4007, 4039
AseI ATTAAT 2 cut(s) 2886, 3675
Asp700I GAANNNNTTC 2 cut(s) 851, 2463
Asp718I GGTACC 1 cut(s) 3114
AspA2I CCTAGG 2 cut(s) 3632, 4564
AspLEI GCGC 1 cut(s) 3004
AsuC2I CCSGG 1 cut(s) 405
AsuNHI GCTAGC 3 cut(s) 3179, 4888, 5468
AvaI CYCGRG 1 cut(s) 514
AvrII CCTAGG 2 cut(s) 3632, 4564
AxyI CCTNAGG 2 cut(s) 1494, 4583
BaeI ACNNNNGTAYC 4 cut(s) 5181, 5214, 5439, 5472
BalI TGGCCA 1 cut(s) 5085
BamHI GGATCC 1 cut(s) 150
BanI GGYRCC 1 cut(s) 3114
BauI CACGAG 1 cut(s) 221
BbrPI CACGTG 2 cut(s) 184, 4670
BbsI GAAGAC 6 cut(s) 509, 1489, 2054, 2804, 3911, 4281
Bbv12I GWGCWC 2 cut(s) 489, 864
BbvCI CCTCAGC 1 cut(s) 5532
BceAI ACGGC 7 cut(s) 305, 479, 493, 633, 1012, 3063, 5119
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciVI GTATCC 2 cut(s) 181, 573
BclI TGATCA 4 cut(s) 1933, 2908, 3160, 4051
BcnI CCSGG 1 cut(s) 405
BcuI ACTAGT 1 cut(s) 5450
BfmI CTRYAG 6 cut(s) 726, 2820, 4431, 4767, 5363, 5379
BfrI CTTAAG 1 cut(s) 2069
BfuAI ACCTGC 2 cut(s) 3126, 4899
BfuI GTATCC 2 cut(s) 181, 573
BglI GCCNNNNNGGC 1 cut(s) 1052
BglII AGATCT 4 cut(s) 641, 1621, 3424, 4976
BlnI CCTAGG 2 cut(s) 3632, 4564
BlpI GCTNAGC 1 cut(s) 4462
BmcAI AGTACT 1 cut(s) 2826
BmeRI GACNNNNNGTC 2 cut(s) 341, 2596
BmeT110I CYCGRG 1 cut(s) 514
BmrI ACTGGG 3 cut(s) 2847, 4674, 5040
BmtI GCTAGC 3 cut(s) 3183, 4892, 5472
BmuI ACTGGG 3 cut(s) 2847, 4674, 5040
BoxI GACNNNNGTC 1 cut(s) 509
BpiI GAAGAC 6 cut(s) 509, 1489, 2054, 2804, 3911, 4281
BplI GAGNNNNNCTC 4 cut(s) 3209, 3241, 3515, 3547
BpmI CTGGAG 6 cut(s) 903, 2741, 2820, 3382, 4014, 4235
Bpu10I CCTNAGC 2 cut(s) 3963, 5532
Bpu1102I GCTNAGC 1 cut(s) 4462
BpuEI CTTGAG 6 cut(s) 113, 254, 680, 2536, 2582, 3116
BpuMI CCSGG 1 cut(s) 405
BsaAI YACGTR 2 cut(s) 184, 4670
BsaBI GATNNNNATC 1 cut(s) 2847
BsaHI GRCGYC 1 cut(s) 365
BsaWI WCCGGW 7 cut(s) 106, 427, 452, 526, 4819, 5173, 5194
Bse118I RCCGGY 3 cut(s) 533, 813, 3750
Bse21I CCTNAGG 2 cut(s) 1494, 4583
Bse3DI GCAATG 4 cut(s) 564, 1065, 2495, 3078
Bse8I GATNNNNATC 1 cut(s) 2847
BseJI GATNNNNATC 1 cut(s) 2847
BseMI GCAATG 4 cut(s) 564, 1065, 2495, 3078
BseRI GAGGAG 6 cut(s) 609, 689, 1094, 1982, 3799, 4670
BseYI CCCAGC 3 cut(s) 1408, 3831, 4039
BsgI GTGCAG 4 cut(s) 343, 2106, 3345, 4758
Bsh1236I CGCG 2 cut(s) 574, 2235
Bsh1285I CGRYCG 1 cut(s) 531
BshNI GGYRCC 1 cut(s) 3114
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 489, 864
BsiHKCI CYCGRG 1 cut(s) 514
BslFI GGGAC 6 cut(s) 899, 2244, 2591, 2618, 4153, 4363
BsmBI CGTCTC 3 cut(s) 999, 2604, 4742
BsmFI GGGAC 6 cut(s) 899, 2244, 2591, 2618, 4153, 4363
BsmI GAATGC 1 cut(s) 1566
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 2 cut(s) 489, 864
Bsp1407I TGTACA 2 cut(s) 2640, 4192
Bsp1720I GCTNAGC 1 cut(s) 4462
BspFNI CGCG 2 cut(s) 574, 2235
BspHI TCATGA 3 cut(s) 843, 1222, 1393
BspMAI CTGCAG 2 cut(s) 4771, 5367
BspMI ACCTGC 2 cut(s) 3126, 4899
BspOI GCTAGC 3 cut(s) 3183, 4892, 5472
BspPI GGATC 4 cut(s) 145, 158, 3297, 5218
BspQI GCTCTTC 2 cut(s) 306, 5364
BspT107I GGYRCC 1 cut(s) 3114
BspTI CTTAAG 1 cut(s) 2069
BsrBI CCGCTC 2 cut(s) 413, 625
BsrDI GCAATG 4 cut(s) 564, 1065, 2495, 3078
BsrFI RCCGGY 3 cut(s) 533, 813, 3750
BsrGI TGTACA 2 cut(s) 2640, 4192
BssAI RCCGGY 3 cut(s) 533, 813, 3750
BssNAI GTATAC 1 cut(s) 2282
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 8 cut(s) 39, 603, 1831, 2573, 3632, 4519, 4564, 5090
Bst1107I GTATAC 1 cut(s) 2282
Bst2BI CACGAG 1 cut(s) 221
Bst6I CTCTTC 8 cut(s) 57, 291, 306, 394, 997, 2468, 5364, 5406
BstACI GRCGYC 1 cut(s) 365
BstAFI CTTAAG 1 cut(s) 2069
BstAPI GCANNNNNTGC 3 cut(s) 836, 1274, 1756
BstAUI TGTACA 2 cut(s) 2640, 4192
BstBAI YACGTR 2 cut(s) 184, 4670
BstDSI CCRYGG 3 cut(s) 141, 572, 1028
BstEII GGTNACC 1 cut(s) 4911
BstENI CCTNNNNNAGG 2 cut(s) 1524, 1654
BstFNI CGCG 2 cut(s) 574, 2235
BstHHI GCGC 1 cut(s) 3004
BstMCI CGRYCG 1 cut(s) 531
BstNSI RCATGY 8 cut(s) 195, 1743, 2379, 2651, 3027, 3623, 4400, 5066
BstPAI GACNNNNGTC 1 cut(s) 509
BstPI GGTNACC 1 cut(s) 4911
BstSFI CTRYAG 6 cut(s) 726, 2820, 4431, 4767, 5363, 5379
BstUI CGCG 2 cut(s) 574, 2235
BstV2I GAAGAC 6 cut(s) 509, 1489, 2054, 2804, 3911, 4281
BstX2I RGATCY 7 cut(s) 150, 641, 1621, 3289, 3424, 4976, 5210
BstXI CCANNNNNNTGG 2 cut(s) 2555, 3343
BstYI RGATCY 7 cut(s) 150, 641, 1621, 3289, 3424, 4976, 5210
BstZ17I GTATAC 1 cut(s) 2282
Bsu36I CCTNAGG 2 cut(s) 1494, 4583
BsuI GTATCC 2 cut(s) 181, 573
BtgI CCRYGG 3 cut(s) 141, 572, 1028
BtsI GCAGTG 3 cut(s) 2118, 4161, 4533
BveI ACCTGC 2 cut(s) 3126, 4899
CaiI CAGNNNCTG 5 cut(s) 2057, 3452, 3992, 4046, 4628
CciI TCATGA 3 cut(s) 843, 1222, 1393
CfoI GCGC 1 cut(s) 3004
Cfr10I RCCGGY 3 cut(s) 533, 813, 3750
Cfr42I CCGCGG 1 cut(s) 575
CseI GACGC 2 cut(s) 2241, 2682
CsiI ACCWGGT 2 cut(s) 3508, 3913
CspCI CAANNNNNGTGG 2 cut(s) 2118, 2153
DraIII CACNNNGTG 1 cut(s) 226
DriI GACNNNNNGTC 2 cut(s) 341, 2596
EaeI YGGCCR 2 cut(s) 580, 5083
Eam1104I CTCTTC 8 cut(s) 57, 291, 306, 394, 997, 2468, 5364, 5406
Eam1105I GACNNNNNGTC 2 cut(s) 341, 2596
EarI CTCTTC 8 cut(s) 57, 291, 306, 394, 997, 2468, 5364, 5406
EciI GGCGGA 2 cut(s) 558, 939
Eco130I CCWWGG 8 cut(s) 39, 603, 1831, 2573, 3632, 4519, 4564, 5090
Eco147I AGGCCT 2 cut(s) 1106, 3221
Eco32I GATATC 2 cut(s) 3845, 5171
Eco57I CTGAAG 8 cut(s) 1542, 1571, 1755, 3251, 3633, 3940, 4446, 5307
Eco72I CACGTG 2 cut(s) 184, 4670
Eco81I CCTNAGG 2 cut(s) 1494, 4583
Eco88I CYCGRG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 4911
EcoNI CCTNNNNNAGG 2 cut(s) 1524, 1654
EcoO109I RGGNCCY 4 cut(s) 233, 679, 1647, 1828
EcoO65I GGTNACC 1 cut(s) 4911
EcoRI GAATTC 2 cut(s) 1323, 1945
EcoRV GATATC 2 cut(s) 3845, 5171
EcoT14I CCWWGG 8 cut(s) 39, 603, 1831, 2573, 3632, 4519, 4564, 5090
EcoT22I ATGCAT 3 cut(s) 2029, 2202, 3829
ErhI CCWWGG 8 cut(s) 39, 603, 1831, 2573, 3632, 4519, 4564, 5090
Esp3I CGTCTC 3 cut(s) 999, 2604, 4742
FalI AAGNNNNNCTT 4 cut(s) 802, 834, 4927, 4959
FaqI GGGAC 6 cut(s) 899, 2244, 2591, 2618, 4153, 4363
FbaI TGATCA 4 cut(s) 1933, 2908, 3160, 4051
FblI GTMKAC 3 cut(s) 345, 1131, 2281
GlaI GCGC 1 cut(s) 3003
GsaI CCCAGC 3 cut(s) 1412, 3835, 4043
GsuI CTGGAG 6 cut(s) 903, 2741, 2820, 3382, 4014, 4235
HgaI GACGC 2 cut(s) 2241, 2682
HhaI GCGC 1 cut(s) 3004
Hin1I GRCGYC 1 cut(s) 365
Hin6I GCGC 1 cut(s) 3002
HinP1I GCGC 1 cut(s) 3002
HincII GTYRAC 4 cut(s) 346, 1132, 1549, 1593
HindII GTYRAC 4 cut(s) 346, 1132, 1549, 1593
HindIII AAGCTT 2 cut(s) 3339, 3952
HpaI GTTAAC 1 cut(s) 1549
Hsp92I GRCGYC 1 cut(s) 365
HspAI GCGC 1 cut(s) 3002
KpnI GGTACC 1 cut(s) 3118
KroI GCCGGC 2 cut(s) 533, 813
KroNI GCCGGC 2 cut(s) 535, 815
Ksp22I TGATCA 4 cut(s) 1933, 2908, 3160, 4051
KspAI GTTAAC 1 cut(s) 1549
KspI CCGCGG 1 cut(s) 575
LguI GCTCTTC 2 cut(s) 306, 5364
MabI ACCWGGT 2 cut(s) 3508, 3913
MbiI CCGCTC 2 cut(s) 413, 625
MfeI CAATTG 2 cut(s) 4143, 5349
MflI RGATCY 7 cut(s) 150, 641, 1621, 3289, 3424, 4976, 5210
MhlI GDGCHC 2 cut(s) 489, 864
MlsI TGGCCA 1 cut(s) 5085
MluI ACGCGT 1 cut(s) 2233
MluNI TGGCCA 1 cut(s) 5085
MlyI GAGTC 6 cut(s) 526, 560, 3341, 4355, 4385, 5249
MmeI TCCRAC 5 cut(s) 358, 1216, 4387, 5240, 5254
Mox20I TGGCCA 1 cut(s) 5085
Mph1103I ATGCAT 3 cut(s) 2029, 2202, 3829
MreI CGCCGGCG 1 cut(s) 533
MroNI GCCGGC 2 cut(s) 533, 813
MroXI GAANNNNTTC 2 cut(s) 851, 2463
MscI TGGCCA 1 cut(s) 5085
MslI CAYNNNNRTG 4 cut(s) 1286, 2502, 3414, 4401
Msp20I TGGCCA 1 cut(s) 5085
MspA1I CMGCKG 9 cut(s) 574, 1373, 1445, 1606, 2408, 3989, 4094, 4772, 5069
MspCI CTTAAG 1 cut(s) 2069
MunI CAATTG 2 cut(s) 4143, 5349
Mva1269I GAATGC 1 cut(s) 1566
MvnI CGCG 2 cut(s) 574, 2235
NaeI GCCGGC 2 cut(s) 535, 815
NciI CCSGG 1 cut(s) 405
NgoMIV GCCGGC 2 cut(s) 533, 813
NheI GCTAGC 3 cut(s) 3179, 4888, 5468
NmeAIII GCCGAG 2 cut(s) 558, 955
NmuCI GTSAC 9 cut(s) 121, 2073, 3753, 4234, 4249, 4665, 4911, 5336, 5519
NsiI ATGCAT 3 cut(s) 2029, 2202, 3829
NspI RCATGY 8 cut(s) 195, 1743, 2379, 2651, 3027, 3623, 4400, 5066
PaeI GCATGC 1 cut(s) 4400
PaeR7I CTCGAG 1 cut(s) 514
PagI TCATGA 3 cut(s) 843, 1222, 1393
PasI CCCWGGG 1 cut(s) 2227
PceI AGGCCT 2 cut(s) 1106, 3221
PciI ACATGT 3 cut(s) 191, 3023, 3619
PciSI GCTCTTC 2 cut(s) 306, 5364
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PctI GAATGC 1 cut(s) 1566
PdiI GCCGGC 2 cut(s) 535, 815
PdmI GAANNNNTTC 2 cut(s) 851, 2463
PflFI GACNNNGTC 2 cut(s) 350, 3915
PflMI CCANNNNNTGG 3 cut(s) 2174, 4712, 4808
PleI GAGTC 6 cut(s) 525, 559, 3340, 4355, 4384, 5248
PmaCI CACGTG 2 cut(s) 184, 4670
PmlI CACGTG 2 cut(s) 184, 4670
PpsI GAGTC 6 cut(s) 525, 559, 3340, 4355, 4384, 5248
Ppu21I YACGTR 2 cut(s) 184, 4670
PpuMI RGGWCCY 3 cut(s) 233, 679, 1647
PscI ACATGT 3 cut(s) 191, 3023, 3619
PshAI GACNNNNGTC 1 cut(s) 509
PshBI ATTAAT 2 cut(s) 2886, 3675
PsiI TTATAA 1 cut(s) 4785
Psp1406I AACGTT 1 cut(s) 2346
Psp5II RGGWCCY 3 cut(s) 233, 679, 1647
PspCI CACGTG 2 cut(s) 184, 4670
PspEI GGTNACC 1 cut(s) 4911
PspFI CCCAGC 3 cut(s) 1408, 3831, 4039
PspPPI RGGWCCY 3 cut(s) 233, 679, 1647
PstI CTGCAG 2 cut(s) 4771, 5367
PstNI CAGNNNCTG 5 cut(s) 2057, 3452, 3992, 4046, 4628
PsuI RGATCY 7 cut(s) 150, 641, 1621, 3289, 3424, 4976, 5210
PsyI GACNNNGTC 2 cut(s) 350, 3915
PvuII CAGCTG 6 cut(s) 1373, 2408, 3989, 4094, 4772, 5069
RseI CAYNNNNRTG 4 cut(s) 1286, 2502, 3414, 4401
SacII CCGCGG 1 cut(s) 575
SalI GTCGAC 2 cut(s) 344, 1130
SapI GCTCTTC 2 cut(s) 306, 5364
ScaI AGTACT 1 cut(s) 2826
SchI GAGTC 6 cut(s) 526, 560, 3341, 4355, 4385, 5249
SduI GDGCHC 2 cut(s) 489, 864
SexAI ACCWGGT 2 cut(s) 3508, 3913
SfcI CTRYAG 6 cut(s) 726, 2820, 4431, 4767, 5363, 5379
Sfr274I CTCGAG 1 cut(s) 514
Sfr303I CCGCGG 1 cut(s) 575
SgrAI CRCCGGYG 1 cut(s) 533
SgrBI CCGCGG 1 cut(s) 575
SlaI CTCGAG 1 cut(s) 514
SmiMI CAYNNNNRTG 4 cut(s) 1286, 2502, 3414, 4401
SmlI CTYRAG 8 cut(s) 128, 269, 514, 659, 2069, 2515, 2561, 3131
SmoI CTYRAG 8 cut(s) 128, 269, 514, 659, 2069, 2515, 2561, 3131
SpeI ACTAGT 1 cut(s) 5450
SphI GCATGC 1 cut(s) 4400
SseBI AGGCCT 2 cut(s) 1106, 3221
SspI AATATT 3 cut(s) 2386, 3326, 4876
StuI AGGCCT 2 cut(s) 1106, 3221
StyI CCWWGG 8 cut(s) 39, 603, 1831, 2573, 3632, 4519, 4564, 5090
TatI WGTACW 6 cut(s) 1616, 2640, 2824, 4192, 4411, 5323
TauI GCSGC 6 cut(s) 549, 574, 577, 830, 955, 3311
TseFI GTSAC 9 cut(s) 121, 2073, 3753, 4234, 4249, 4665, 4911, 5336, 5519
Tsp45I GTSAC 9 cut(s) 121, 2073, 3753, 4234, 4249, 4665, 4911, 5336, 5519
TspGWI ACGGA 7 cut(s) 158, 323, 642, 686, 927, 3379, 5223
Tth111I GACNNNGTC 2 cut(s) 350, 3915
Van91I CCANNNNNTGG 3 cut(s) 2174, 4712, 4808
Vha464I CTTAAG 1 cut(s) 2069
VspI ATTAAT 2 cut(s) 2886, 3675
XagI CCTNNNNNAGG 2 cut(s) 1524, 1654
XapI RAATTY 8 cut(s) 869, 1089, 1323, 1945, 3261, 3556, 3586, 5405
XbaI TCTAGA 1 cut(s) 961
XceI RCATGY 8 cut(s) 195, 1743, 2379, 2651, 3027, 3623, 4400, 5066
XcmI CCANNNNNNNNNTGG 1 cut(s) 5502
XhoI CTCGAG 1 cut(s) 514
XmaJI CCTAGG 2 cut(s) 3632, 4564
XmiI GTMKAC 3 cut(s) 345, 1131, 2281
XmnI GAANNNNTTC 2 cut(s) 851, 2463
ZraI GACGTC 1 cut(s) 366
ZrmI AGTACT 1 cut(s) 2826
Zsp2I ATGCAT 3 cut(s) 2029, 2202, 3829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.