Rmu_sc0000575.1_g000017

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000575.1
OG0011973
Physical Location & Seq
Reverse (-)
98297 .. 99391
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000575.1_g000017.1.cds

Sequence Viewer

Length: 1095 bp
atgatatcacctgttgtagctatagttataattgatcatcgtatttctgatgcttcccaaagtccaaataccacagctgctgaatgtggaccatcagaccccctagggccagacagttgccccaggacttcctctgattcagaacctccatctgctttaactgagcctagaaccaacaatccttctaatacaggggagggaaagtcacgtgatttacccaatggcgacatagttaaaccagtgccaactggtggggaaaaggtagatgaacaagaaactggaaaggagacgagcaaaaaactttctgcagccgcacctccttttaatccatcccccattccagtttttggctcaattccggttccagttccaggtctcaaagatcatggagggattttgcccccacctgtaaatattcctccgatgcttgcagttaatcctgtccgtagatcaccccatcagtcagcgacagcaagagttccttatggtccacgactttctggtggctataacagatctggaagtcgggtttcacgtaataaacatagctttcacaatggtgagcacaccggggatggtcctcccagaataatgaatccacatgcagctgagtttgtacctggccaaccttgggttcaaaatggctatccactgtctcccaatggtttcttgtcttccccaaatggttatccagtgtccccaaatggatatccggtatcaccgaatggtactccggtgaatcaaaacgggtctccgacatctccagtcagttcagacgagtcatcaccaattgtgactgctgatattggtgttggagcaaatactgaaggtgcagggaaggagactaatgataagttatcagtacaagttgaatgtgacaagcaaccaattgaaggagagctgcaggaagaacagtctttagataatgtaaacgttcctcctgaaactgaagaaaagcctattgataccgatacagttcctggtgatactattgtggaaaaggagactagcaatctagtagtagaggaaaaaccaagcaaatgttggggagattatagcgataatgaagctgaggtcattgaggttagtagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

364

Amino Acids

38.23

Weight (kDa)

4.68

Isoelectric Point (pI)

60.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 29
AccB7I CCANNNNNTGG 2 cut(s) 251, 347
AciI CCGC 1 cut(s) 312
AclI AACGTT 1 cut(s) 933
AcoI YGGCCR 1 cut(s) 622
AcuI CTGAAG 2 cut(s) 846, 969
AcvI CACGTG 1 cut(s) 209
AfaI GTAC 3 cut(s) 618, 730, 864
AfiI CCNNNNNNNGG 5 cut(s) 251, 347, 371, 631, 893
AgsI TTSAA 3 cut(s) 638, 872, 893
AjnI CCWGG 4 cut(s) 122, 370, 619, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AleI CACNNNNGTG 1 cut(s) 558
AloI GAACNNNNNNTCC 2 cut(s) 163, 195
AluBI AGCT 6 cut(s) 20, 77, 549, 608, 901, 1070
AluI AGCT 6 cut(s) 20, 77, 549, 608, 901, 1070
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 6 cut(s) 281, 380, 660, 756, 836, 998
AlwNI CAGNNNCTG 2 cut(s) 80, 980
AoxI GGCC 2 cut(s) 107, 622
ApeKI GCWGC 4 cut(s) 77, 308, 605, 901
AspA2I CCTAGG 1 cut(s) 103
AspS9I GGNCC 4 cut(s) 89, 107, 488, 578
AsuC2I CCSGG 1 cut(s) 571
AsuHPI GGTGA 6 cut(s) 444, 572, 711, 748, 777, 995
AvaII GGWCC 3 cut(s) 89, 488, 578
AvrII CCTAGG 1 cut(s) 103
BaeI ACNNNNGTAYC 4 cut(s) 720, 753, 978, 1011
BalI TGGCCA 1 cut(s) 624
BbrPI CACGTG 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 567
BbvCI CCTCAGC 1 cut(s) 1071
BbvI GCAGC 4 cut(s) 64, 320, 617, 888
BccI CCATC 5 cut(s) 100, 157, 337, 465, 569
BciT130I CCWGG 4 cut(s) 124, 372, 621, 981
BclI TGATCA 1 cut(s) 34
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 6 cut(s) 281, 380, 660, 756, 836, 998
BfaI CTAG 4 cut(s) 104, 168, 1008, 1016
BfmI CTRYAG 3 cut(s) 21, 306, 902
BglII AGATCT 1 cut(s) 515
BisI GCNGC 5 cut(s) 78, 309, 312, 606, 902
BlnI CCTAGG 1 cut(s) 103
BlsI GCNGC 5 cut(s) 79, 310, 313, 607, 903
Bme1390I CCNGG 5 cut(s) 124, 372, 571, 621, 981
Bme18I GGWCC 3 cut(s) 89, 488, 578
BmgT120I GGNCC 4 cut(s) 89, 107, 488, 578
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 5 cut(s) 124, 372, 571, 621, 981
BmsI GCATC 2 cut(s) 40, 414
BpiI GAAGAC 1 cut(s) 666
BpmI CTGGAG 1 cut(s) 747
Bpu10I CCTNAGC 1 cut(s) 1071
BpuMI CCSGG 1 cut(s) 571
BsaAI YACGTR 2 cut(s) 209, 536
BsaI GGTCTC 2 cut(s) 380, 756
BsaJI CCNNGG 4 cut(s) 103, 122, 570, 629
BsaWI WCCGGW 3 cut(s) 358, 712, 733
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 5 cut(s) 251, 347, 371, 631, 893
Bse1I ACTGG 7 cut(s) 239, 253, 283, 341, 365, 692, 764
BseBI CCWGG 4 cut(s) 124, 372, 621, 981
BseDI CCNNGG 4 cut(s) 103, 122, 570, 629
BseGI GGATG 2 cut(s) 329, 580
BseLI CCNNNNNNNGG 5 cut(s) 251, 347, 371, 631, 893
BseMII CTCAG 3 cut(s) 153, 600, 1062
BseNI ACTGG 7 cut(s) 239, 253, 283, 341, 365, 692, 764
BseXI GCAGC 4 cut(s) 64, 320, 617, 888
BsgI GTGCAG 1 cut(s) 852
BshFI GGCC 2 cut(s) 109, 624
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 4 cut(s) 359, 570, 713, 734
BslFI GGGAC 1 cut(s) 682
BslI CCNNNNNNNGG 5 cut(s) 251, 347, 371, 631, 893
BsmAI GTCTC 6 cut(s) 281, 380, 660, 756, 836, 998
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 682
BsnI GGCC 2 cut(s) 109, 624
Bso31I GGTCTC 2 cut(s) 380, 756
Bsp1286I GDGCHC 1 cut(s) 567
Bsp143I GATC 4 cut(s) 34, 382, 449, 515
BspACI CCGC 1 cut(s) 312
BspANI GGCC 2 cut(s) 109, 624
BspCNI CTCAG 3 cut(s) 154, 601, 1063
BspLI GGNNCC 1 cut(s) 363
BspMAI CTGCAG 2 cut(s) 310, 906
BspTNI GGTCTC 2 cut(s) 380, 756
BsrI ACTGG 7 cut(s) 239, 253, 283, 341, 365, 692, 764
BssECI CCNNGG 4 cut(s) 103, 122, 570, 629
BssMI GATC 4 cut(s) 34, 382, 449, 515
BssT1I CCWWGG 2 cut(s) 103, 629
Bst2UI CCWGG 4 cut(s) 124, 372, 621, 981
Bst4CI ACNGT 4 cut(s) 116, 654, 915, 976
BstBAI YACGTR 2 cut(s) 209, 536
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 3 cut(s) 162, 609, 1071
BstF5I GGATG 2 cut(s) 329, 580
BstKTI GATC 4 cut(s) 37, 385, 452, 518
BstMAI GTCTC 6 cut(s) 281, 380, 660, 756, 836, 998
BstMBI GATC 4 cut(s) 34, 382, 449, 515
BstNI CCWGG 4 cut(s) 124, 372, 621, 981
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 5 cut(s) 122, 370, 569, 619, 979
BstSFI CTRYAG 3 cut(s) 21, 306, 902
BstV1I GCAGC 4 cut(s) 64, 320, 617, 888
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 1 cut(s) 515
BstYI RGATCY 1 cut(s) 515
BsuRI GGCC 2 cut(s) 109, 624
BtsCI GGATG 2 cut(s) 329, 580
BtsIMutI CAGTG 3 cut(s) 246, 650, 699
Cac8I GCNNGC 1 cut(s) 429
CaiI CAGNNNCTG 2 cut(s) 80, 980
Cfr13I GGNCC 4 cut(s) 89, 107, 488, 578
Csp6I GTAC 3 cut(s) 617, 729, 863
CviAII CATG 2 cut(s) 386, 602
CviQI GTAC 3 cut(s) 617, 729, 863
DdeI CTNAG 3 cut(s) 162, 609, 1071
DpnI GATC 4 cut(s) 36, 384, 451, 517
DpnII GATC 4 cut(s) 34, 382, 449, 515
EaeI YGGCCR 1 cut(s) 622
Eco130I CCWWGG 2 cut(s) 103, 629
Eco31I GGTCTC 2 cut(s) 380, 756
Eco32I GATATC 2 cut(s) 6, 710
Eco47I GGWCC 3 cut(s) 89, 488, 578
Eco57I CTGAAG 2 cut(s) 846, 969
Eco72I CACGTG 1 cut(s) 209
EcoRII CCWGG 4 cut(s) 122, 370, 619, 979
EcoRV GATATC 2 cut(s) 6, 710
EcoT14I CCWWGG 2 cut(s) 103, 629
ErhI CCWWGG 2 cut(s) 103, 629
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 2 cut(s) 389, 605
FaiI YATR 9 cut(s) 23, 29, 230, 387, 486, 510, 546, 603, 1056
FalI AAGNNNNNCTT 2 cut(s) 466, 498
FaqI GGGAC 1 cut(s) 682
FatI CATG 2 cut(s) 385, 601
FbaI TGATCA 1 cut(s) 34
Fnu4HI GCNGC 5 cut(s) 78, 309, 312, 606, 902
FokI GGATG 2 cut(s) 316, 587
Fsp4HI GCNGC 5 cut(s) 78, 309, 312, 606, 902
FspBI CTAG 4 cut(s) 104, 168, 1008, 1016
GluI GCNGC 5 cut(s) 78, 309, 312, 606, 902
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 2 cut(s) 109, 624
HapII CCGG 4 cut(s) 359, 570, 713, 734
Hin1II CATG 2 cut(s) 389, 605
HinfI GANTC 4 cut(s) 137, 595, 739, 779
HpaII CCGG 4 cut(s) 359, 570, 713, 734
HphI GGTGA 6 cut(s) 444, 572, 711, 748, 777, 995
Hpy166II GTNNAC 3 cut(s) 89, 491, 931
Hpy188I TCNGA 7 cut(s) 49, 97, 136, 142, 423, 756, 775
Hpy188III TCNNGA 2 cut(s) 519, 941
Hpy8I GTNNAC 3 cut(s) 89, 491, 931
HpyAV CCTTC 4 cut(s) 192, 821, 832, 887
HpyCH4III ACNGT 4 cut(s) 116, 654, 915, 976
HpyCH4IV ACGT 3 cut(s) 208, 535, 933
HpyCH4V TGCA 5 cut(s) 308, 431, 605, 833, 904
HpyF3I CTNAG 3 cut(s) 162, 609, 1071
HpySE526I ACGT 3 cut(s) 208, 535, 933
Hsp92II CATG 2 cut(s) 389, 605
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 4 cut(s) 34, 382, 449, 515
LmnI GCTCC 1 cut(s) 815
Lsp1109I GCAGC 4 cut(s) 64, 320, 617, 888
LweI GCATC 2 cut(s) 40, 414
MaeI CTAG 4 cut(s) 104, 168, 1008, 1016
MaeII ACGT 3 cut(s) 208, 535, 933
MaeIII GTNAC 3 cut(s) 204, 793, 875
MalI GATC 4 cut(s) 36, 384, 451, 517
MboI GATC 4 cut(s) 34, 382, 449, 515
MboII GAAGA 3 cut(s) 666, 920, 962
MfeI CAATTG 2 cut(s) 789, 888
MflI RGATCY 1 cut(s) 515
MhlI GDGCHC 1 cut(s) 567
MlsI TGGCCA 1 cut(s) 624
MluCI AATT 4 cut(s) 30, 354, 789, 888
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 2 cut(s) 779, 793
Mox20I TGGCCA 1 cut(s) 624
MscI TGGCCA 1 cut(s) 624
MseI TTAA 4 cut(s) 158, 234, 324, 435
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 624
MspA1I CMGCKG 2 cut(s) 77, 608
MspI CCGG 4 cut(s) 359, 570, 713, 734
MspR9I CCNGG 5 cut(s) 124, 372, 571, 621, 981
MunI CAATTG 2 cut(s) 789, 888
MvaI CCWGG 4 cut(s) 124, 372, 621, 981
NciI CCSGG 1 cut(s) 571
NdeII GATC 4 cut(s) 34, 382, 449, 515
NlaIII CATG 2 cut(s) 389, 605
NlaIV GGNNCC 1 cut(s) 363
NmuCI GTSAC 3 cut(s) 204, 793, 875
NspI RCATGY 1 cut(s) 605
OliI CACNNNNGTG 1 cut(s) 558
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PfeI GAWTC 3 cut(s) 137, 595, 739
PflMI CCANNNNNTGG 2 cut(s) 251, 347
PkrI GCNGC 5 cut(s) 79, 310, 313, 607, 903
PleI GAGTC 1 cut(s) 787
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
PpsI GAGTC 1 cut(s) 787
Ppu21I YACGTR 2 cut(s) 209, 536
PsiI TTATAA 1 cut(s) 29
Psp1406I AACGTT 1 cut(s) 933
Psp6I CCWGG 4 cut(s) 122, 370, 619, 979
PspCI CACGTG 1 cut(s) 209
PspGI CCWGG 4 cut(s) 122, 370, 619, 979
PspN4I GGNNCC 1 cut(s) 363
PspPI GGNCC 4 cut(s) 89, 107, 488, 578
PstI CTGCAG 2 cut(s) 310, 906
PstNI CAGNNNCTG 2 cut(s) 80, 980
PsuI RGATCY 1 cut(s) 515
PvuII CAGCTG 2 cut(s) 77, 608
RsaI GTAC 3 cut(s) 618, 730, 864
RsaNI GTAC 3 cut(s) 617, 729, 863
RseI CAYNNNNRTG 1 cut(s) 558
SaqAI TTAA 4 cut(s) 158, 234, 324, 435
SatI GCNGC 5 cut(s) 78, 309, 312, 606, 902
Sau3AI GATC 4 cut(s) 34, 382, 449, 515
Sau96I GGNCC 4 cut(s) 89, 107, 488, 578
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 5 cut(s) 124, 372, 571, 621, 981
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 2 cut(s) 40, 414
SfcI CTRYAG 3 cut(s) 21, 306, 902
SinI GGWCC 3 cut(s) 89, 488, 578
SmiMI CAYNNNNRTG 1 cut(s) 558
Sse9I AATT 4 cut(s) 30, 354, 789, 888
SsiI CCGC 1 cut(s) 312
SspI AATATT 1 cut(s) 415
SspMI CTAG 4 cut(s) 104, 168, 1008, 1016
StyD4I CCNGG 5 cut(s) 122, 370, 569, 619, 979
StyI CCWWGG 2 cut(s) 103, 629
TaaI ACNGT 4 cut(s) 116, 654, 915, 976
TaiI ACGT 3 cut(s) 211, 538, 936
TasI AATT 4 cut(s) 30, 354, 789, 888
TatI WGTACW 1 cut(s) 862
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 3 cut(s) 137, 595, 739
Tru1I TTAA 4 cut(s) 158, 234, 324, 435
Tru9I TTAA 4 cut(s) 158, 234, 324, 435
TscAI CASTG 3 cut(s) 246, 657, 699
TseFI GTSAC 3 cut(s) 204, 793, 875
TseI GCWGC 4 cut(s) 77, 308, 605, 901
Tsp45I GTSAC 3 cut(s) 204, 793, 875
TspDTI ATGAA 3 cut(s) 282, 608, 1080
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 3 cut(s) 246, 657, 699
Van91I CCANNNNNTGG 2 cut(s) 251, 347
VpaK11BI GGWCC 3 cut(s) 89, 488, 578
XceI RCATGY 1 cut(s) 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 1041
XmaJI CCTAGG 1 cut(s) 103
XspI CTAG 4 cut(s) 104, 168, 1008, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.