Rh4BG126800

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011973
Physical Location & Seq
Reverse (-)
21163050 .. 21180308
17259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG126800.1

Sequence Viewer

Length: 4998 bp
ATGGCTCCCAAAACAGGGAAAGCCAAGCCACACAAATCCAAGGGAGACAAGAAGAAGAAAGAAGAGAAAGTTTTGCCTACCGTAATCGAGATTACCATTGAAACACCAGATGACTCGCAAGTGATCCTCAAGGTTCGGGTTTGTAGTGGGAAGCCGACGACTATAGTAGCATCCAGAAAAGGGTTCTACCCTGCTGGAAAACGTCCTCTTATTACTCACTCTCTGGTTGCTTTGCTTCAACAGATAAGCCGGCCGTTCGATGCTGCATACAATGCTGTCATGAAGGCTTTCACCGAGCACAATAAATTTGGGAACCTTCCTTATGGTTTTCGGGCAAACACATGGGTTGTCCCTCCGGTTGTTGCTGAGAACCCGTCTGTTTTCCCGCCACTTCCTCTAGAAGATGAAAATTGGGGAGGAAATGGGGGTGGGCAGGGAAGAGATGGTAAACACGATAACAGGCCATGGGCGAAAGAGTTTGCCATTCTTGCAGCAATGCCTTGTGCAACAGCAGAGGAGAGGCAAGTTCGAGATCGAAAGGCCTTTCTGCTTCACAGTTTATTTGTCGACGTCTCAGTTCTTAAAGCTGTTGCAGCTATTAAGAGCCTAATTGACACTCATCAATTTTCCCTAAATGATTCCACTCTTTCACTTCATCATGAGGAAAAAATTGGAGATTTAATCATTAAGATCGTTAGAGATGCACCTGATGCGAGTGTAAAGGTGGATTGCAAGAATGGTGGTAGTCGAGTTCTAGGAATGCCACAAGAAGAAGTTACTCAGAGAAACCTACTCAAAGGCATAACAGCTGATGAGAGTGCAACTGTTCATGATACTTCTACACTAGGTGTTGTGGTTGTTAGGCATTGTGGATTTACTGCTGTTGTGAAAGTTTTAGCTGAGGTGAATTGGGTGGGGAAACCTGTTCCTCAGGACATTGAAATTGAGGACCAGCCTGAAGGAGGTGCAAATGCCTTGAATGTTAACAGCCTGAGAATGCTATTGCAACAGTCATCCCTCCTTCAGTCATCAACTACGGTTCAACGAAGCCAGAGTACAGATTTTGAAAATTTACGTTCTGCTAGGTCCTTAGTAAGGAAAGTACTCGAAGAAAGTTTATTAAGGTTGCAGGGTGGGCCATCTAATCACACCAAGTCGATCAGATGGGAGCTAGGAGCATGTTGGGTGCAACATTTGCAAAATCAGGCTTCAGCGAAAAATGAGCCTAAGAAAAATGAAGAAGCTAAGATAGAACCGGCTGTTAAGGGCCTCGGAAAGCAAGGTGGACTGCTAAAGGAAATAAAGAAGAAAATGGATATCAGGAGCAATAAAACTGAACAGGGCAAGGAAGTTCTTGGGGGTAACAACCTTGATCTTAATAAGAATTCAGTCACCACCAGTCAGGAGGAGTTAGAAAAGCGAGATGCCGAAAAGGAAATAAATTGGAGAAGGCTACTTCCTGATGCATCCTATTCGCGGCTCAAAGAATCAGATACCGGTCTCCACCTTAAGTCACCTGATGAGTTGATTGAAATGGCACACAAGTATTACGCTGATACTGCACTTCCAAAACTGGTGGCAGATTTTGGCTCCCTTGAACTTTCACCAGTTGATGGAAGGACATTGACAGATTTTATGCATACAAGGGGTTTGCAAATGTGTTCCCTGGGACGCGTGGTTGAACTTGCAGATAAGCTCCCTCATGTACAATCACTCTGTATCCATGAGATGGTTGTGCGAGCATACAAACACATACTGCAAGCTGTTGTAGCAGCAGTTGATAATGTCGCAGACTTGGCTGCATCCATAGCAGCATGTCTAAATATTCTGCTAGGAACCCCATCACCTGAAAATGTGGATGGAGACATTACTTGTGAGGATACGTTAAAATGGAAGTGGGTGGAAACATTCCTTTTGAAGAGGTTTGGATGGCAATGGAAACATGAAAGCGTTGAGGACTTAAGAAAGTTTGCCATTCTTCGTGGATTGTGCCATAAGGTTGGCCTTGAGCTTGTGCCAAGGGACTATGATATGGACACTTCGTCTCCTTTTAGGAAATCAGATATAGTAAGCATGGTCCCTGTGTACAAGCATGTTGCATGTTCATCTGCTGATGGACGTACACTTCTGGAATCTTCCAAGACTTCCTTGGATAAAGGTAAACTGGAGGATGCTGTTAATTATGGCACTAAGGCGTTGTCAAAACTTGTATCAGTATGTGGGCCTTATCATCGAATGACTGCTGGAGCATATAGTCTTCTCGCTGTAGTACTGTATCACACTGGGGATTTTAATCAGGCAACCATCTATCAGCAAAAAGCATTGGATATTAATGAGAGAGAGCTTGGACTTGATCATCCTGATACAATGAAAAGTTACGGGGATTTAGCTGTGTTCTACTACCGACTTCAACATACAGAGTTGGCCTTGAAGTACGTCAATCGTGCGCTGTATCTTTTGCACCTAACATGTGGGCCGTCTCATCCAAACACGGCTGCCACCTATATCAATGTAGCAATGATGGAAGAGGGTTTGGGGAACGTCCATGTTGCTCTTAGGTACCTCCATGAAGCTCTCAAGTGCAATCAAAGGCTACTTGGAGCTGATCATATACAGACTGCTGCTAGCTATCATGCCATAGCTATCGCACTTTCTTTGATGGAGGCATACTCGTTAAGTGTTCAGCACGAACAAACTACCTTGCAAATTCTGCAGGCGAAACTCGGGTCAGAGGATCTACGCACACAGGATGCGGCTGCATGGCTTGAGTATTTTGAGTCCAAAGCTTTGGAGCAGCAAGAAGCTGCACGGAATGGTACTCCAAAGCCAGATGCTTCAATCTCCAGCAAAGGTCATTTAAGTGTATCAGATCTTTTGGATTATATAACACCAGATGCTGATATGAAAGCTAGAGAGGCCCAAAGGAAAGCTCGTTTGAAGGTTAAAGGCAAACCAGGTCAGAACGGGGAGGCAGTCTCTGATGAGTACCAGAAGGATGAAAATTTGTTACCAAGTCATCCTGTTGCAGAGAATTTAAGTGATAAAGAGAACAAGTCTGAAGCACAATTAGGAGAACCTAGGAATGAGAAATATGATTCCCATCTACTTGAGCAATCAATAATATTTGCTACACATGATGATCTGGCACAAGATGACAACTCAGATGAAGGCTGGCAAGAGGCTGTTCCTAAAGGCCGGTCACTTATAGGTCGCAAATCTACTGGGTCAAGGAGGCCAAGCCTTGAAAAACTGAATACTAACTTTATCAATACATCCCAGTCATCAAGATATCGAGGCAAGGCCAATAATTTCACATCCCCCAAAACAAGCCCAAATGAGCCTGCTTCTTCAACTGGACCTGCTCCTCCAGTGTCAAAGAAGTTTGTCAAGAGTGCAAGCTTCAGTCCTAAGCAAAACAACTCTAGCACACCAGCTACCGGAGCTGCAGAGAGATTGCCAAACCCAAAGTCAGCACCATCTACCCCAGCTTCTGTTGATCAAGTCGTCAAGTCTGTAAGTCCGATCAGTGTTCAGTCAGCTGGAAAACTCTTTTCCTACAAAGAAGTTGCATTGGCTCCACCTGGGACAATTGTGAAGGCAGTGGCGGAGCAGTCACCAAAGCTGATTGTACAAATTTCTCAGGTGGGCCAGGAGACACCTACAACAGAGGTCAGTGTTGGTGAAGTGACGGCAATTAAAGATGTGAAGGATCAAAAAAATAAGAAATCGACAGGAGAGAAACAGGTTATGGTTTCTGAGAAAAGCCCTTCTGTAGTACGATCAGCAAAACTGAAGGATTCTGTGGCAAAGGAGTCCCTGGAAGTTGTGAAGCATGCTAGTGTTGGAGTACAAGTTGAAGCTGAATCTGAAGCTGTGGAATTAGAAAACACTGCTTTTGAAGGTGCAGAAGTTGAAAATGTAAAAGTTCCTGTTCCGAGTGTTGAGATTTCTGATGCTTCCCAAAGTCCAAATACCACAGCTGCTGAATGTGGACCATCAGACCCCCTAGGGCCAGACAGTTGCCCCAGGACTTCCTCTGATTCAGAACCTCCATCTGCTTTAACTGAGACTAGAACCAACAATCCTTCTAATACAGGGGAGGGAAAGTCACGTGATTTACCCAATGGCGACATAGTTAAACCAGTGCCAACTGGTGGGGAAAAGGTAGATGAACAAGAAACTGGAAAGGAGACGAGCAAAAAACTTTCTGCAGCCGCACCTCCTTTTAATCCATCCCCCATTCCAGTTTTTGGCTCAATTCCGGTTCCAGTTCCAGGTCTCAATGATCATGGAGGGATTTTGCCCCCACCTGTAAATATTCCTCCGATGCTTGCAGTTAATCCTGTCCGTAGATCACCTCATCAGTCAGCGACAGCAAGAGTTCCTTATGGTCCACGACTTTCTGGTGGCTATAACAGATCTGGAAGTCGGGTTTCACGTAATAAACATAGCTTTCACAATGGTGAGCACACCGGGGATGGTCCTCCCAGAATAATGAACCCACATGCAGCTGAGTTTGTACCTGGCCAACCTTGGGTTCAAAATGGCTATCCAGTGTCTCCCAATGGTTTCTTGCCTTCCCCAAATGGTTATCCAGTGTCCCCAAATGGATATGCGGTATCACCGAATGGTGCTCCGGTGAATCAAAACGGGTCTCCGACATCTCCAGTCAGTTCAGATGAGTCATCACCAGTTGTGACTGCTGATATTGGTGTTGGAGCAAATACTGAAGGTGCAGCAAAGGAGACTGATGATAAGTTATCAGTACAAGTTGAATGTGACAAGCAACCAATTGAAGGAGAGCTGCAGGAAGAACAGTCTTTAGATAATGTAAACGTTCCACCTGAAACTGAAGAAAAGCCTATTGATACCGATACAGTTCCTGGTGATACTATTGTGGAAAAGGAGACTAGCAATCTAGTAGTTGAGGAAAAACCAAGCAAATGTTGGGGAGATTATAGCGATAATGAAGCTGAGGTCATTGAGGTTAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1665

Amino Acids

180.83

Weight (kDa)

6.01

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_p135 PF12807 534 - 677 2.9e-23 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 718 - 778 2.6e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 718 - 817 2.5e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 747 - 817 1.3e-12 Tetratricopeptide repeat
TPR_10 PF13374 747 - 788 1.9e-09 Tetratricopeptide repeat
TPR_12 PF13424 831 - 905 1.8e-10 Tetratricopeptide repeat
TPR_10 PF13374 831 - 871 3.7e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 573
Acc36I ACCTGC 1 cut(s) 3367
Acc65I GGTACC 1 cut(s) 2559
AccB1I GGYRCC 1 cut(s) 2559
AccB7I CCANNNNNTGG 4 cut(s) 1613, 1729, 4154, 4250
AccI GTMKAC 1 cut(s) 567
AccII CGCG 2 cut(s) 1477, 1674
AciI CCGC 6 cut(s) 386, 1477, 2753, 3605, 4215, 4616
AclI AACGTT 1 cut(s) 4836
AclWI GGATC 3 cut(s) 118, 2742, 3717
AcoI YGGCCR 2 cut(s) 251, 4525
AcsI RAATTY 7 cut(s) 305, 1069, 1384, 2706, 3001, 3031, 3633
AcuI CTGAAG 9 cut(s) 978, 1007, 1194, 3078, 3385, 3812, 3888, 4749, 4872
AcvI CACGTG 1 cut(s) 4112
AcyI GRCGYC 1 cut(s) 570
AdeI CACNNNGTG 1 cut(s) 848
AflII CTTAAG 2 cut(s) 1508, 1960
AflIII ACRYGT 2 cut(s) 1672, 2468
AgeI ACCGGT 1 cut(s) 1496
AhdI GACNNNNNGTC 1 cut(s) 2041
AjnI CCWGG 9 cut(s) 1665, 2953, 3580, 3648, 3816, 4025, 4273, 4522, 4882
AjuI GAANNNNNNNTTGG 2 cut(s) 4521, 4553
AleI CACNNNNGTG 1 cut(s) 4461
AloI GAACNNNNNNTCC 2 cut(s) 4066, 4098
Alw21I GWGCWC 3 cut(s) 300, 4470, 4636
AlwI GGATC 3 cut(s) 118, 2742, 3717
AlwNI CAGNNNCTG 5 cut(s) 2897, 2978, 3491, 3983, 4883
Ama87I CYCGRG 1 cut(s) 2723
ApoI RAATTY 7 cut(s) 305, 1069, 1384, 2706, 3001, 3031, 3633
ArsI GACNNNNNNTTYG 4 cut(s) 2711, 2743, 3452, 3484
AseI ATTAAT 1 cut(s) 2331
AsiGI ACCGGT 1 cut(s) 1496
Asp700I GAANNNNTTC 1 cut(s) 287
Asp718I GGTACC 1 cut(s) 2559
AspA2I CCTAGG 2 cut(s) 3077, 4006
AspLEI GCGC 1 cut(s) 2449
AsuC2I CCSGG 1 cut(s) 4474
AsuNHI GCTAGC 1 cut(s) 2624
AvaI CYCGRG 1 cut(s) 2723
AvaII GGWCC 7 cut(s) 949, 1086, 2077, 3356, 3992, 4391, 4481
AvrII CCTAGG 2 cut(s) 3077, 4006
AxyI CCTNAGG 1 cut(s) 930
BaeI ACNNNNGTAYC 2 cut(s) 4881, 4914
BalI TGGCCA 1 cut(s) 4527
BanI GGYRCC 1 cut(s) 2559
BarI GAAGNNNNNNTAC 2 cut(s) 1039, 1071
BbrPI CACGTG 1 cut(s) 4112
BbsI GAAGAC 1 cut(s) 2249
Bbv12I GWGCWC 3 cut(s) 300, 4470, 4636
BbvCI CCTCAGC 2 cut(s) 900, 4974
BceAI ACGGC 4 cut(s) 238, 2461, 2508, 3705
BcgI CGANNNNNNTGC 2 cut(s) 1455, 1489
BciT130I CCWGG 9 cut(s) 1667, 2955, 3582, 3650, 3818, 4027, 4275, 4524, 4884
BciVI GTATCC 2 cut(s) 1730, 1873
BclI TGATCA 4 cut(s) 2353, 2605, 3496, 4285
BcnI CCSGG 1 cut(s) 4474
BfmI CTRYAG 7 cut(s) 162, 2265, 2711, 3444, 3771, 4209, 4805
BfrI CTTAAG 2 cut(s) 1508, 1960
BfuAI ACCTGC 1 cut(s) 3367
BfuI GTATCC 2 cut(s) 1730, 1873
BglII AGATCT 2 cut(s) 2869, 4418
BlnI CCTAGG 2 cut(s) 3077, 4006
BmcAI AGTACT 2 cut(s) 1104, 2271
Bme18I GGWCC 7 cut(s) 949, 1086, 2077, 3356, 3992, 4391, 4481
BmeRI GACNNNNNGTC 1 cut(s) 2041
BmeT110I CYCGRG 1 cut(s) 2723
BmiI GGNNCC 8 cut(s) 6, 314, 1591, 1837, 2079, 2561, 3576, 4266
BmrI ACTGGG 3 cut(s) 2292, 3231, 3271
BmtI GCTAGC 1 cut(s) 2628
BmuI ACTGGG 3 cut(s) 2292, 3231, 3271
BpiI GAAGAC 1 cut(s) 2249
BplI GAGNNNNNCTC 4 cut(s) 2654, 2686, 2960, 2992
BpmI CTGGAG 5 cut(s) 2186, 2265, 2827, 3351, 4650
Bpu10I CCTNAGC 3 cut(s) 900, 3408, 4974
BpuEI CTTGAG 5 cut(s) 113, 2027, 2561, 2786, 3128
BpuMI CCSGG 1 cut(s) 4474
BsaAI YACGTR 2 cut(s) 4112, 4439
BsaBI GATNNNNATC 2 cut(s) 2292, 3099
BsaHI GRCGYC 1 cut(s) 570
BsaI GGTCTC 3 cut(s) 1505, 4283, 4659
BsaWI WCCGGW 5 cut(s) 355, 1496, 3437, 4261, 4636
BsaXI ACNNNNNCTCC 6 cut(s) 2029, 2059, 3837, 3867, 4091, 4121
Bse118I RCCGGY 4 cut(s) 249, 1255, 1496, 3195
Bse21I CCTNAGG 1 cut(s) 930
Bse3DI GCAATG 3 cut(s) 501, 1940, 2523
Bse8I GATNNNNATC 2 cut(s) 2292, 3099
BseBI CCWGG 9 cut(s) 1667, 2955, 3582, 3650, 3818, 4027, 4275, 4524, 4884
BseJI GATNNNNATC 2 cut(s) 2292, 3099
BseMI GCAATG 3 cut(s) 501, 1940, 2523
BseRI GAGGAG 3 cut(s) 530, 1421, 3354
BseX3I CGGCCG 1 cut(s) 251
BseYI CCCAGC 1 cut(s) 3484
BsgI GTGCAG 4 cut(s) 1545, 2790, 3924, 4755
Bsh1236I CGCG 2 cut(s) 1477, 1674
Bsh1285I CGRYCG 1 cut(s) 254
BshNI GGYRCC 1 cut(s) 2559
BshTI ACCGGT 1 cut(s) 1496
BsiEI CGRYCG 1 cut(s) 254
BsiHKAI GWGCWC 3 cut(s) 300, 4470, 4636
BsiHKCI CYCGRG 1 cut(s) 2723
BsiSI CCGG 9 cut(s) 250, 356, 1256, 1497, 3196, 3438, 4262, 4473, 4637
BslFI GGGAC 7 cut(s) 335, 1683, 2036, 2063, 3598, 3799, 4585
BsmBI CGTCTC 4 cut(s) 577, 2049, 2484, 4184
BsmFI GGGAC 7 cut(s) 335, 1683, 2036, 2063, 3598, 3799, 4585
BsmI GAATGC 2 cut(s) 765, 1002
Bso31I GGTCTC 3 cut(s) 1505, 4283, 4659
BsoBI CYCGRG 1 cut(s) 2723
Bsp1286I GDGCHC 3 cut(s) 300, 4470, 4636
Bsp1407I TGTACA 3 cut(s) 1705, 2085, 3628
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 6 cut(s) 386, 1477, 2753, 3605, 4215, 4616
BspFNI CGCG 2 cut(s) 1477, 1674
BspHI TCATGA 3 cut(s) 279, 658, 829
BspLI GGNNCC 8 cut(s) 6, 314, 1591, 1837, 2079, 2561, 3576, 4266
BspMAI CTGCAG 4 cut(s) 2715, 3448, 4213, 4809
BspMI ACCTGC 1 cut(s) 3367
BspOI GCTAGC 1 cut(s) 2628
BspPI GGATC 3 cut(s) 118, 2742, 3717
BspT107I GGYRCC 1 cut(s) 2559
BspTI CTTAAG 2 cut(s) 1508, 1960
BspTNI GGTCTC 3 cut(s) 1505, 4283, 4659
BsrDI GCAATG 3 cut(s) 501, 1940, 2523
BsrFI RCCGGY 4 cut(s) 249, 1255, 1496, 3195
BsrGI TGTACA 3 cut(s) 1705, 2085, 3628
BssAI RCCGGY 4 cut(s) 249, 1255, 1496, 3195
BssNI GRCGYC 1 cut(s) 570
BssT1I CCWWGG 7 cut(s) 39, 464, 2018, 2148, 3077, 4006, 4532
Bst2UI CCWGG 9 cut(s) 1667, 2955, 3582, 3650, 3818, 4027, 4275, 4524, 4884
Bst4CI ACNGT 9 cut(s) 82, 557, 826, 1011, 1039, 2274, 4019, 4818, 4879
Bst6I CTCTTC 4 cut(s) 57, 433, 1913, 2520
BstACI GRCGYC 1 cut(s) 570
BstAFI CTTAAG 2 cut(s) 1508, 1960
BstAPI GCANNNNNTGC 4 cut(s) 272, 710, 1195, 2710
BstAUI TGTACA 3 cut(s) 1705, 2085, 3628
BstBAI YACGTR 2 cut(s) 4112, 4439
BstDSI CCRYGG 1 cut(s) 464
BstENI CCTNNNNNAGG 2 cut(s) 960, 1093
BstFNI CGCG 2 cut(s) 1477, 1674
BstHHI GCGC 1 cut(s) 2449
BstMCI CGRYCG 1 cut(s) 254
BstNI CCWGG 9 cut(s) 1667, 2955, 3582, 3650, 3818, 4027, 4275, 4524, 4884
BstNSI RCATGY 7 cut(s) 1182, 1818, 2096, 2103, 2472, 3836, 4508
BstSFI CTRYAG 7 cut(s) 162, 2265, 2711, 3444, 3771, 4209, 4805
BstUI CGCG 2 cut(s) 1477, 1674
BstV2I GAAGAC 1 cut(s) 2249
BstX2I RGATCY 3 cut(s) 2734, 2869, 4418
BstXI CCANNNNNNTGG 2 cut(s) 2000, 2788
BstYI RGATCY 3 cut(s) 2734, 2869, 4418
BstZI CGGCCG 1 cut(s) 251
Bsu36I CCTNAGG 1 cut(s) 930
BsuI GTATCC 2 cut(s) 1730, 1873
BtgI CCRYGG 1 cut(s) 464
BtsI GCAGTG 2 cut(s) 3606, 3888
BtsIMutI CAGTG 9 cut(s) 2280, 3375, 3532, 3606, 3679, 3888, 4149, 4560, 4602
BveI ACCTGC 1 cut(s) 3367
CaiI CAGNNNCTG 5 cut(s) 2897, 2978, 3491, 3983, 4883
CciI TCATGA 3 cut(s) 279, 658, 829
CfoI GCGC 1 cut(s) 2449
Cfr10I RCCGGY 4 cut(s) 249, 1255, 1496, 3195
CseI GACGC 1 cut(s) 1680
CsiI ACCWGGT 1 cut(s) 2953
CspAI ACCGGT 1 cut(s) 1496
CspCI CAANNNNNGTGG 4 cut(s) 721, 756, 1557, 1592
DraIII CACNNNGTG 1 cut(s) 848
DriI GACNNNNNGTC 1 cut(s) 2041
EaeI YGGCCR 2 cut(s) 251, 4525
EagI CGGCCG 1 cut(s) 251
Eam1104I CTCTTC 4 cut(s) 57, 433, 1913, 2520
Eam1105I GACNNNNNGTC 1 cut(s) 2041
EarI CTCTTC 4 cut(s) 57, 433, 1913, 2520
EciI GGCGGA 1 cut(s) 3620
EclXI CGGCCG 1 cut(s) 251
Eco130I CCWWGG 7 cut(s) 39, 464, 2018, 2148, 3077, 4006, 4532
Eco147I AGGCCT 1 cut(s) 542
Eco31I GGTCTC 3 cut(s) 1505, 4283, 4659
Eco32I GATATC 2 cut(s) 1318, 3290
Eco47I GGWCC 7 cut(s) 949, 1086, 2077, 3356, 3992, 4391, 4481
Eco52I CGGCCG 1 cut(s) 251
Eco57I CTGAAG 9 cut(s) 978, 1007, 1194, 3078, 3385, 3812, 3888, 4749, 4872
Eco72I CACGTG 1 cut(s) 4112
Eco81I CCTNAGG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 2723
EcoNI CCTNNNNNAGG 2 cut(s) 960, 1093
EcoO109I RGGNCCY 2 cut(s) 1086, 1267
EcoRI GAATTC 1 cut(s) 1384
EcoRII CCWGG 9 cut(s) 1665, 2953, 3580, 3648, 3816, 4025, 4273, 4522, 4882
EcoRV GATATC 2 cut(s) 1318, 3290
EcoT14I CCWWGG 7 cut(s) 39, 464, 2018, 2148, 3077, 4006, 4532
EcoT22I ATGCAT 2 cut(s) 1468, 1641
ErhI CCWWGG 7 cut(s) 39, 464, 2018, 2148, 3077, 4006, 4532
Esp3I CGTCTC 4 cut(s) 577, 2049, 2484, 4184
FalI AAGNNNNNCTT 4 cut(s) 2132, 2164, 4369, 4401
FaqI GGGAC 7 cut(s) 335, 1683, 2036, 2063, 3598, 3799, 4585
FauI CCCGC 1 cut(s) 393
FbaI TGATCA 4 cut(s) 2353, 2605, 3496, 4285
FblI GTMKAC 1 cut(s) 567
GlaI GCGC 1 cut(s) 2448
GsaI CCCAGC 1 cut(s) 3488
GsuI CTGGAG 5 cut(s) 2186, 2265, 2827, 3351, 4650
HapII CCGG 9 cut(s) 250, 356, 1256, 1497, 3196, 3438, 4262, 4473, 4637
HgaI GACGC 1 cut(s) 1680
HhaI GCGC 1 cut(s) 2449
Hin1I GRCGYC 1 cut(s) 570
Hin6I GCGC 1 cut(s) 2447
HinP1I GCGC 1 cut(s) 2447
HincII GTYRAC 2 cut(s) 568, 985
HindII GTYRAC 2 cut(s) 568, 985
HindIII AAGCTT 2 cut(s) 2784, 3397
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 9 cut(s) 250, 356, 1256, 1497, 3196, 3438, 4262, 4473, 4637
Hpy99I CGWCG 2 cut(s) 160, 572
HpyCH4III ACNGT 9 cut(s) 82, 557, 826, 1011, 1039, 2274, 4019, 4818, 4879
Hsp92I GRCGYC 1 cut(s) 570
HspAI GCGC 1 cut(s) 2447
KpnI GGTACC 1 cut(s) 2563
KroI GCCGGC 1 cut(s) 249
KroNI GCCGGC 1 cut(s) 251
Ksp22I TGATCA 4 cut(s) 2353, 2605, 3496, 4285
KspAI GTTAAC 1 cut(s) 985
MabI ACCWGGT 1 cut(s) 2953
MfeI CAATTG 2 cut(s) 3588, 4791
MflI RGATCY 3 cut(s) 2734, 2869, 4418
MhlI GDGCHC 3 cut(s) 300, 4470, 4636
MlsI TGGCCA 1 cut(s) 4527
MluI ACGCGT 1 cut(s) 1672
MluNI TGGCCA 1 cut(s) 4527
MlyI GAGTC 4 cut(s) 107, 2786, 3821, 4691
MmeI TCCRAC 3 cut(s) 3823, 4682, 4696
Mox20I TGGCCA 1 cut(s) 4527
Mph1103I ATGCAT 2 cut(s) 1468, 1641
MroNI GCCGGC 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 287
MscI TGGCCA 1 cut(s) 4527
MslI CAYNNNNRTG 3 cut(s) 2859, 3837, 4461
Msp20I TGGCCA 1 cut(s) 4527
MspA1I CMGCKG 4 cut(s) 809, 3539, 3980, 4511
MspCI CTTAAG 2 cut(s) 1508, 1960
MspI CCGG 9 cut(s) 250, 356, 1256, 1497, 3196, 3438, 4262, 4473, 4637
MunI CAATTG 2 cut(s) 3588, 4791
Mva1269I GAATGC 2 cut(s) 765, 1002
MvaI CCWGG 9 cut(s) 1667, 2955, 3582, 3650, 3818, 4027, 4275, 4524, 4884
MvnI CGCG 2 cut(s) 1477, 1674
NaeI GCCGGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 4474
NcoI CCATGG 1 cut(s) 464
NgoMIV GCCGGC 1 cut(s) 249
NheI GCTAGC 1 cut(s) 2624
NlaIV GGNNCC 8 cut(s) 6, 314, 1591, 1837, 2079, 2561, 3576, 4266
NmuCI GTSAC 8 cut(s) 1390, 1512, 3198, 3612, 3685, 4107, 4696, 4778
NsiI ATGCAT 2 cut(s) 1468, 1641
NspI RCATGY 7 cut(s) 1182, 1818, 2096, 2103, 2472, 3836, 4508
OliI CACNNNNGTG 1 cut(s) 4461
PaeI GCATGC 1 cut(s) 3836
PagI TCATGA 3 cut(s) 279, 658, 829
PasI CCCWGGG 1 cut(s) 1666
PceI AGGCCT 1 cut(s) 542
PciI ACATGT 1 cut(s) 2468
PcsI WCGNNNNNNNCGW 1 cut(s) 4435
PctI GAATGC 2 cut(s) 765, 1002
PdiI GCCGGC 1 cut(s) 251
PdmI GAANNNNTTC 1 cut(s) 287
PfeI GAWTC 8 cut(s) 638, 1487, 2132, 3095, 3797, 3863, 4040, 4642
PflMI CCANNNNNTGG 4 cut(s) 1613, 1729, 4154, 4250
PinAI ACCGGT 1 cut(s) 1496
PleI GAGTC 4 cut(s) 107, 2785, 3820, 4690
PmaCI CACGTG 1 cut(s) 4112
PmlI CACGTG 1 cut(s) 4112
PpsI GAGTC 4 cut(s) 107, 2785, 3820, 4690
Ppu21I YACGTR 2 cut(s) 4112, 4439
PpuMI RGGWCCY 1 cut(s) 1086
PscI ACATGT 1 cut(s) 2468
PshBI ATTAAT 1 cut(s) 2331
Psp1406I AACGTT 1 cut(s) 4836
Psp5II RGGWCCY 1 cut(s) 1086
Psp6I CCWGG 9 cut(s) 1665, 2953, 3580, 3648, 3816, 4025, 4273, 4522, 4882
PspCI CACGTG 1 cut(s) 4112
PspFI CCCAGC 1 cut(s) 3484
PspGI CCWGG 9 cut(s) 1665, 2953, 3580, 3648, 3816, 4025, 4273, 4522, 4882
PspN4I GGNNCC 8 cut(s) 6, 314, 1591, 1837, 2079, 2561, 3576, 4266
PspPPI RGGWCCY 1 cut(s) 1086
PstI CTGCAG 4 cut(s) 2715, 3448, 4213, 4809
PstNI CAGNNNCTG 5 cut(s) 2897, 2978, 3491, 3983, 4883
PsuI RGATCY 3 cut(s) 2734, 2869, 4418
PvuII CAGCTG 4 cut(s) 809, 3539, 3980, 4511
RseI CAYNNNNRTG 3 cut(s) 2859, 3837, 4461
SalI GTCGAC 1 cut(s) 566
ScaI AGTACT 2 cut(s) 1104, 2271
SchI GAGTC 4 cut(s) 107, 2786, 3821, 4691
SduI GDGCHC 3 cut(s) 300, 4470, 4636
SexAI ACCWGGT 1 cut(s) 2953
SfcI CTRYAG 7 cut(s) 162, 2265, 2711, 3444, 3771, 4209, 4805
SinI GGWCC 7 cut(s) 949, 1086, 2077, 3356, 3992, 4391, 4481
SmiMI CAYNNNNRTG 3 cut(s) 2859, 3837, 4461
SmlI CTYRAG 7 cut(s) 128, 1508, 1960, 2006, 2576, 2765, 3107
SmoI CTYRAG 7 cut(s) 128, 1508, 1960, 2006, 2576, 2765, 3107
SphI GCATGC 1 cut(s) 3836
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 6 cut(s) 386, 1477, 2753, 3605, 4215, 4616
SspI AATATT 3 cut(s) 1825, 3123, 4318
StuI AGGCCT 1 cut(s) 542
StyI CCWWGG 7 cut(s) 39, 464, 2018, 2148, 3077, 4006, 4532
TaaI ACNGT 9 cut(s) 82, 557, 826, 1011, 1039, 2274, 4019, 4818, 4879
TatI WGTACW 8 cut(s) 1055, 1102, 1705, 2085, 2269, 3628, 3847, 4765
TauI GCSGC 3 cut(s) 1480, 2756, 4217
TfiI GAWTC 8 cut(s) 638, 1487, 2132, 3095, 3797, 3863, 4040, 4642
TscAI CASTG 9 cut(s) 2287, 3375, 3532, 3606, 3679, 3895, 4149, 4560, 4602
TseFI GTSAC 8 cut(s) 1390, 1512, 3198, 3612, 3685, 4107, 4696, 4778
Tsp45I GTSAC 8 cut(s) 1390, 1512, 3198, 3612, 3685, 4107, 4696, 4778
TspGWI ACGGA 2 cut(s) 2824, 4337
TspRI CASTG 9 cut(s) 2287, 3375, 3532, 3606, 3679, 3895, 4149, 4560, 4602
Van91I CCANNNNNTGG 4 cut(s) 1613, 1729, 4154, 4250
Vha464I CTTAAG 2 cut(s) 1508, 1960
VpaK11BI GGWCC 7 cut(s) 949, 1086, 2077, 3356, 3992, 4391, 4481
VspI ATTAAT 1 cut(s) 2331
XagI CCTNNNNNAGG 2 cut(s) 960, 1093
XapI RAATTY 7 cut(s) 305, 1069, 1384, 2706, 3001, 3031, 3633
XbaI TCTAGA 1 cut(s) 397
XceI RCATGY 7 cut(s) 1182, 1818, 2096, 2103, 2472, 3836, 4508
XcmI CCANNNNNNNNNTGG 2 cut(s) 2146, 4944
XmaJI CCTAGG 2 cut(s) 3077, 4006
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 287
ZraI GACGTC 1 cut(s) 571
ZrmI AGTACT 2 cut(s) 1104, 2271
Zsp2I ATGCAT 2 cut(s) 1468, 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.