MD16G1258600.v1.1

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011973
Physical Location & Seq
Forward (+)
31248430 .. 31270876
22447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1258600.v1.1.491

Sequence Viewer

Length: 5391 bp
ATGGCTCCCAAGACTGGCAAGGCCAAGCCCCATAAACCCAAGGGAGACAAGAAGAAAAAGGAGGAGAAAGTTTTGCCTACCGTAATCGAGATTAGCATCGAAACACCAGATGAGCTACAAGTAACTCTCAAGGGCATCTCTACGGACACGATCCTGGACGTGAGAAAGCTGTTGGGAGTCCACGTGGAGACATGTCATTTGACCAATTTCTCCTTATCACACGAGGTGCGAAATCCTAATCAGGTGCGAGGGCCCAAACTCAAAGATTCGGTCGACATCCTCTCCCTTAAACCCTCCCACCTCACCATCGTCGAAGATGACTACACAGAAGAGCAAGCCGTGGCACACATCCGGCGACTGATGGACATCGTCGCTTGCACGACATCTTTTGGTTCGTCATCCGCTCCTTCACCTAAAACGCCTGGCCGGTCAAACTCAAGGGGCGACGTACCGCAGCACAAAAACGACGACGGGCCCAATGCAGATGCAAGGGCCAAAGTTTTGGGTTCAATTCCGATCGTTGATGCCGAGTCATCTGCTGCCTCGACTGTGTCAATGTATCCTCCTCCAAAGCTCGGGCAGTTCTACGATTTCTTTTCCTTGTCTCACCTTACGCCTCCTCTCCACTACATAAGAAGATCTACGCGTCCGTTCCTTGAGGATAAGACAGAAGACGACTTGTTTCAAATGGACATTCGGGTTTGTAGCGGGAAGCCGACGACAATCGTGGCTTCCAGTAAAGGATTCTACCCTGCTGGAAAACGAGCTCTAATGGTTCACTCTTTGGTTGTTCTTCTTCAGCAAATTAGTAGGCCATTTGATGTTGCATACAACGCTGTTATGAAGGCTTTCATTGAGCACAATAAATTTGGAAACCTTCCCTACGGTTTTCGCGCAAACACATGGGTTGTCCCTCCAATTGTTGCTGATAAACCGTCAGTTTTCCCATCACTTCCTTTGGAAGACGAAAACTGGGGAGGAAACGGAGGTGGTCAGGGAAGAGACGGTAAACATGATTACAGTCCATGGGCAAAAGAGTTTGCCATTTTAAAAGTAATGCCTTGCTCAACATCAGAGGAGAGGCAAGTCCGAGACCGCAAGGCTTTTTTGCTTCACAGTTTATTTGTGGACGTCTCTGTTCTTAAAGCTGTTACAGCAATTAAGCGTCTAGTTGAGACTAACCATTGTGCCTTAAATGATGCCACTTTTTCACTTGTTCACGAGGAAAGTGTTGGAGATTTAATCGTTAAGGTGACCAGAAATATGCCCGATGCAAGTATAAAGGTAGACTGTAAAATCGATGGCAGCCAAGTTCTTGGAATTCCGCAAGAACAAGTTACTCAGAGAAACCTACTCAAAGGAATAACAGCTGACGAAAGTGCAACTGTTCATGATACTCCTACACTGGGTGTTGTGGTTGTCAGACTTTGTGGGTTTACGGCTGTTGTGAGGGTTTCAAGTGAGGTGAATTGGGAAGGAAAACCTGTTCCTAAGGACGTTGAAATTGAGGACCAGCTTGAAGGTGGTGCTAATGCTTTGAATGTTAATAGCTTGAGACTGTTATTGCAACAGTCCTCCCTGCCTCAATCATCAAATGAAGTTCTACGTACAAATTTTGAAGGTATACATACTACTAGATCTCTCGTGAAGAAAGTACTTGAAGACAGTCTGTTAAGATTGCAGGATGGACCCACTAATAATACTAAGTCCATTAGATGGGAGCTAGGAGCATGTTGGGTACAACATTTGCAAAATCAAGCTTCGGGAAAAACTGAATCTAAAAAAACTGAACAAGCTAAGATTGAGCCGGTTGTTAAGGGTCTTGGAAAGCAAGGGGGGTTACTAAAGGAAATTAAAAAGAAAATGGATGTCAGGAGCAACAAAACTGACCAGGTGAAGGATGTTGTTGGGGTTAACAACCTTGATATACATCAGAATTCTGATATCACTACTCAGGAGGAATTAGAAAAGCGAGATGCAGAAAAGGAAACTATTTGGAGAAAGATGCTTCCTGAATCGTCATATTTGCGCCTCAAAGAATCAGAAACTGGTCTTCACCTTCAGTCACCCGATGAGTTGATTGAAATGGCACATAAATACTATGTTGACACTGCACTTCCAAAACTGGTTGCAGATTTTGGCTCCCTTGAACTTTCACCAGTCGATGGAAGGACATTGACAGATTTTATGCATACTAGGGGTTTGCAATTGTCTTCCCTAGGACGTGTGGTTGAACTTGCAGATAAGCTCCCTCATGTACGATCACTTTGTATCCATGAGATGGTTGTGCGAGCCTATAAACACATACTCCAAGCAGTTGTAGCAGCAGTTGATAATATTGCAGACTTGGCTGCATCAATAGCGGCATGTTTAAATATATTGCTAGGAACACCAGCAACTGAAAATGGGGATGTAGATATTACTTATGATGATGCATTAAAATGGAAATGGGTGGAAAGTTTCCTTTTGAAGAGGTTTGGGTGGCAATGGAAACACGAAACTGTTAAGGATCTAAGGAAGTTTGCCATTCTCCGTGGATTGTCCCACAAGGTTGGACTTGAGCTTGTTCCTAGGGACTACGATATGGACAATGTATCTCCTTTTCGGAAATCAGATATAGTAAGCATGGTCCCTGTATATAAGCACGTTGCATGTTCATCAGCGGATGGGCGTACACTCTTAGAATCGTCCAAGACTTTGTTGGATAAAGGTAAACTGGAGGAAGCGGTTAATTTTGGCACTAAGGCACTCTCAAAACTAGTGTCCGTATGTGGGCCTTATCATCGAATGACAGCGGGAGCATATAGTCTTCTTGCTGTAGTATTGTACCACACTGGGGATTTCAACCAGGCGACCATTTATCAGCAAAAGGCATTGGATATCAATGAGAGGGAGCTTGGACTTGATCATCCTGATACGATGAAAAGTTATGGGGATTTGGCTGTTTTCTATTACAGACTTCAACATACAGAGTTGGCCCTCAAGTACGTCAATCGTGCTTTGTATCTTTTGCATCTAACATGTGGGCCGTCTCATCCAAATACGGCTGCCACCTATATTAACGTGGCAATGATGGAAGAAGGTTTGGGGAATGTCCATGTTGCTCTTAGGTATCTCCATGAAGCGCTAAAGTGCAATCAAAGGCTACTTGGACCTGATCATATACAGACCGCTGCTAGCTATCATGCCATTGCTATAGCACTCTCTTTGATGGAAGCTTACACATTAAGTGTTCAGCACGAACAAACTACCTTACAGATACTGCAAGCAAAACTTGGCTCTGAGGATCTACGCACACAGGATGCCACTGCATGGCTTGAGTATTTCGAGTCTAAGGCTTTGGAGCAGCAAGAAGCTGCACGCAATGGTACTCCAAAGCCAGATGCCTCTATCTCCAGCAAAGGACATTTAAGTGTGTCAGACCTTTTGGATTATATAACACCAGATGCTGATATGAAAGCAAGGGATGCAAGAAAAGCTCGTGCAAAGGTTAAAGGAAAACCAGGAAAAAACGAGGCAATAGTCTCAGATGGATACCAGAAGGACGACATTTTGTTACCAAGTCAGCTTGTTGCTGAGAATTCAGGTGATAAAGAGAACCAGTCTGCACCTCCATTTGCAGAACCTAAGCATGAGAAAGTTGATACAAGTCTACTGGATCAATCAATAATATTCGAAAAAAAAGATGATGCGGCACAAGATGATGCCTCAGATGAAGGTTGGCAAGAAGCAGTTCCTAAAGGTCGTTCACCCATGGGTCGCAAATCCTTTCTGTCAAGGAGACCAAGCCTCAAAAAACTGAATACTAATTTTGTGCATGCATCCCAATTATCACGATACAATGGAAAGCCTAATAGTTTGACATCTCCAAAAACAAGCCCAATTGAGCCTGCTGCTTTTCCTGGACCTGCTCTTCCCGTTTCAAAAAAGTTTGTTAAGAGTGCAAGCTTCAGTCCTAAGCCAAGCAACTCTAGCATGTCAGTTAGTGGAGGGACAGAGAAGCACTCAAACCCAAAGTCAGCTCCTTCTACTCCAGCTTCTATTGATCAAGTTACCAAGTCATCTTCTGTGACAAATCCAATCAGTGTTCAGTCAACTGGAAAACTTTTCTCCTATAAAGAAGTTGCATTGGCTCCTCCTGGAACAATTGTGAAGGCAGTGGGAGAGCAATTACCAAAAGGAAATGTTCCTATTGTACAACTTTCTAAGGTTGGCCAGGAGAAAACTGCAACAGATGTTACCGGTGAAGTGATACTAGTTAAAGATGTCGAGGAAGAGGAAAAGCAAAAACTTGTGGGAGAGAAAGTGGTTTTGGCTCTTGAGGAAAATGGTATTGATGTAAAACATACAAAAGTGCAGAACTCTGTGGCAAGGAAATCTCTGGGAGTTGTAAAGCATGCTAGTGTTGGAGTACAAGTGGAAGCTGAGATCATAGAACTGAAAACCACTGCTTATGAAGAAGGACAAGTAGAAAATGCCGAAGTCACAGTTTCAAACGGTGAGATTTCTATTACTTCCCAACCCGCAAACACTACTCAAAATGGACAATTGCAAACTCAAGCGCTGGACAGTTCGACCAAGGTTTGCCCTGATTCAGAACCTTCATTTGTTTTAACTGAAAGCACAACTCACTTTATGGAAGAAAATTCTTCCCTTTTGGAAGCAAAGGTTGAAGAAGATGGAAGTCCACATGATTTCTCTGATGGTGGGATAGTTAAGCAAGGGCCAACTGATGGAGAAAAGTTAGATGAACCAGAAACTAAAAAAGAGACCACCAAGAAACTTTCTGCAGCCGCACCACCTTTCAATCCATCCCTAATACCAGTTTTTGGTTCAGTTCCAGTGGCAGGTTCCAAAGATCATGGAGGGATTTTGCCCCCACCAGTAAATATTCCTCCTATGCTTACGATCAGTCCTGTTCGTAGATCACCTCATCAGTCAGCAACAGCAAGGGTTCCGTATGGTCCACGATTGGCTGGTGGATATAATAGATCTGGAAGTCGGGCTTCACGTAATAAACATGTCTTCCACAATGGTGAGAACACAGGGGATGGAAATCACTTTAGTCCACCTAGAGTAATGAACCCACATGCGGCAGAGTTTGTACCTGGCCAACCTTGGATTCCCAATGGCTATCCAGCATCTCCTAGTGATTTTTTGCCTCCCCCAAGTGGTTATCCCATGTCCCCAAATGGATATCCATCGTCGCGTAATGATATTCCAGTGACTCAAAATGTGTTCCCAACATCTCCAATCGGTTCAGAAGAGTCATTAACAATTATAAATGCTCCACTTGGTGTTGAAACAAATATTAAAGGTGAAGCAAAAGAGAATAATGACATGTCTTCATTAGAAGTTAGATGCGAGACGCAAAAAATTGAAGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1797

Amino Acids

196.56

Weight (kDa)

6.34

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 48 - 124 1.4e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 718 - 861 8.8e-22 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 899 - 962 5.6e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 901 - 1001 2.2e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 931 - 1001 1.4e-12 Tetratricopeptide repeat
TPR_10 PF13374 931 - 972 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 1015 - 1089 2.4e-11 Tetratricopeptide repeat
TPR_10 PF13374 1015 - 1055 1.1e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5282
AatII GACGTC 1 cut(s) 1134
Acc36I ACCTGC 2 cut(s) 3916, 4838
AccB7I CCANNNNNTGG 6 cut(s) 1716, 2165, 2281, 3312, 4733, 4829
AccBSI CCGCTC 1 cut(s) 404
AccI GTMKAC 4 cut(s) 273, 1287, 1624, 3652
AccII CGCG 3 cut(s) 646, 894, 5209
AclWI GGATC 4 cut(s) 145, 2517, 3294, 3666
AcoI YGGCCR 3 cut(s) 424, 4213, 5110
AcsI RAATTY 6 cut(s) 866, 1320, 1612, 1936, 3580, 4645
AcuI CTGAAG 3 cut(s) 782, 2045, 3934
AcvI CACGTG 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 1131
AdeI CACNNNGTG 3 cut(s) 226, 1409, 5297
AfeI AGCGCT 2 cut(s) 3126, 4563
AflIII ACRYGT 6 cut(s) 191, 644, 2224, 3020, 5020, 5340
AgeI ACCGGT 1 cut(s) 4241
AhlI ACTAGT 2 cut(s) 2758, 4255
AjiI CACGTC 2 cut(s) 160, 2225
AjnI CCWGG 9 cut(s) 153, 421, 1890, 2847, 3502, 3901, 4138, 4215, 5107
AjuI GAANNNNNNNTTGG 4 cut(s) 4295, 4327, 5106, 5138
AleI CACNNNNGTG 1 cut(s) 5034
AloI GAACNNNNNNTCC 2 cut(s) 1470, 1502
Alw21I GWGCWC 2 cut(s) 769, 861
AlwI GGATC 4 cut(s) 145, 2517, 3294, 3666
AlwNI CAGNNNCTG 4 cut(s) 2048, 2399, 3262, 3449
Ama87I CYCGRG 1 cut(s) 575
Aor51HI AGCGCT 2 cut(s) 3126, 4563
ApaI GGGCCC 2 cut(s) 255, 477
ApoI RAATTY 6 cut(s) 866, 1320, 1612, 1936, 3580, 4645
ArsI GACNNNNNNTTYG 8 cut(s) 181, 213, 255, 287, 2747, 2779, 4001, 4033
AsiGI ACCGGT 1 cut(s) 4241
Asp700I GAANNNNTTC 2 cut(s) 848, 3732
AspA2I CCTAGG 2 cut(s) 2218, 2570
AspLEI GCGC 4 cut(s) 896, 2031, 3127, 4564
AsuII TTCGAA 1 cut(s) 3675
AsuNHI GCTAGC 1 cut(s) 3176
AvaI CYCGRG 1 cut(s) 575
AvaII GGWCC 6 cut(s) 1510, 1688, 2629, 3152, 3905, 4964
AvrII CCTAGG 2 cut(s) 2218, 2570
AxyI CCTNAGG 1 cut(s) 1491
BaeGI GKGCMC 2 cut(s) 255, 477
BalI TGGCCA 2 cut(s) 4215, 5112
BanII GRGCYC 3 cut(s) 255, 477, 769
BauI CACGAG 4 cut(s) 221, 1220, 1643, 3480
BbrPI CACGTG 1 cut(s) 184
BbsI GAAGAC 8 cut(s) 678, 969, 1668, 2045, 2205, 2801, 5017, 5337
Bbv12I GWGCWC 2 cut(s) 769, 861
BceAI ACGGC 4 cut(s) 323, 1455, 3013, 3060
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BciT130I CCWGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
BciVI GTATCC 3 cut(s) 570, 2282, 3527
BclI TGATCA 3 cut(s) 2905, 3157, 4045
BcuI ACTAGT 2 cut(s) 2758, 4255
BfmI CTRYAG 3 cut(s) 2817, 3195, 4788
BfoI RGCGCY 2 cut(s) 3128, 4565
BfuAI ACCTGC 2 cut(s) 3916, 4838
BfuI GTATCC 3 cut(s) 570, 2282, 3527
BglII AGATCT 3 cut(s) 638, 1637, 4991
BlnI CCTAGG 2 cut(s) 2218, 2570
BmcAI AGTACT 1 cut(s) 1656
Bme1390I CCNGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
Bme18I GGWCC 6 cut(s) 1510, 1688, 2629, 3152, 3905, 4964
BmeT110I CYCGRG 1 cut(s) 575
BmgBI CACGTC 2 cut(s) 160, 2225
BmiI GGNNCC 9 cut(s) 6, 253, 475, 1690, 2143, 2631, 4134, 4852, 4956
BmrFI CCNGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
BmrI ACTGGG 3 cut(s) 982, 1415, 2844
BmtI GCTAGC 1 cut(s) 3180
BmuI ACTGGG 3 cut(s) 982, 1415, 2844
BpiI GAAGAC 8 cut(s) 678, 969, 1668, 2045, 2205, 2801, 5017, 5337
BplI GAGNNNNNCTC 2 cut(s) 3989, 4021
BpmI CTGGAG 3 cut(s) 2738, 3379, 4017
Bpu10I CCTNAGC 2 cut(s) 3627, 3957
Bpu14I TTCGAA 1 cut(s) 3675
BpuEI CTTGAG 9 cut(s) 113, 421, 677, 1573, 2579, 2966, 3338, 4340, 4542
Bsa29I ATCGAT 1 cut(s) 1299
BsaAI YACGTR 3 cut(s) 184, 1607, 5012
BsaBI GATNNNNATC 5 cut(s) 95, 365, 1929, 5055, 5200
BsaHI GRCGYC 1 cut(s) 1131
BsaI GGTCTC 3 cut(s) 1086, 3775, 4763
BsaJI CCNNGG 9 cut(s) 39, 339, 1025, 2218, 2533, 2570, 3753, 4578, 5117
BsaWI WCCGGW 1 cut(s) 4241
Bse118I RCCGGY 3 cut(s) 426, 1807, 4241
Bse21I CCTNAGG 1 cut(s) 1491
Bse3DI GCAATG 4 cut(s) 2492, 3075, 3189, 3370
Bse8I GATNNNNATC 5 cut(s) 95, 365, 1929, 5055, 5200
BseBI CCWGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
BseCI ATCGAT 1 cut(s) 1299
BseDI CCNNGG 9 cut(s) 39, 339, 1025, 2218, 2533, 2570, 3753, 4578, 5117
BseJI GATNNNNATC 5 cut(s) 95, 365, 1929, 5055, 5200
BseMI GCAATG 4 cut(s) 2492, 3075, 3189, 3370
BseMII CTCAG 7 cut(s) 1355, 1967, 3273, 3540, 3567, 3723, 4416
BseRI GAGGAG 5 cut(s) 77, 555, 609, 1091, 4125
BseSI GKGCMC 2 cut(s) 255, 477
BsgI GTGCAG 4 cut(s) 2097, 3342, 3591, 4376
Bsh1236I CGCG 3 cut(s) 646, 894, 5209
Bsh1285I CGRYCG 2 cut(s) 273, 519
BshTI ACCGGT 1 cut(s) 4241
BshVI ATCGAT 1 cut(s) 1299
BsiEI CGRYCG 2 cut(s) 273, 519
BsiHKAI GWGCWC 2 cut(s) 769, 861
BsiHKCI CYCGRG 1 cut(s) 575
BsiSI CCGG 4 cut(s) 352, 427, 1808, 4242
BslFI GGGAC 6 cut(s) 896, 2527, 2588, 2615, 4006, 5170
BsmBI CGTCTC 4 cut(s) 996, 1138, 3036, 5360
BsmFI GGGAC 6 cut(s) 896, 2527, 2588, 2615, 4006, 5170
Bso31I GGTCTC 3 cut(s) 1086, 3775, 4763
BsoBI CYCGRG 1 cut(s) 575
Bsp119I TTCGAA 1 cut(s) 3675
Bsp120I GGGCCC 2 cut(s) 251, 473
Bsp1286I GDGCHC 4 cut(s) 255, 477, 769, 861
Bsp1407I TGTACA 1 cut(s) 4195
Bsp19I CCATGG 2 cut(s) 1025, 3753
BspCNI CTCAG 7 cut(s) 1354, 1966, 3274, 3539, 3568, 3722, 4417
BspDI ATCGAT 1 cut(s) 1299
BspFNI CGCG 3 cut(s) 646, 894, 5209
BspHI TCATGA 1 cut(s) 1390
BspLI GGNNCC 9 cut(s) 6, 253, 475, 1690, 2143, 2631, 4134, 4852, 4956
BspMAI CTGCAG 1 cut(s) 4792
BspMI ACCTGC 2 cut(s) 3916, 4838
BspOI GCTAGC 1 cut(s) 3180
BspPI GGATC 4 cut(s) 145, 2517, 3294, 3666
BspQI GCTCTTC 2 cut(s) 324, 3918
BspT104I TTCGAA 1 cut(s) 3675
BspTNI GGTCTC 3 cut(s) 1086, 3775, 4763
BsrBI CCGCTC 1 cut(s) 404
BsrDI GCAATG 4 cut(s) 2492, 3075, 3189, 3370
BsrFI RCCGGY 3 cut(s) 426, 1807, 4241
BsrGI TGTACA 1 cut(s) 4195
BssAI RCCGGY 3 cut(s) 426, 1807, 4241
BssECI CCNNGG 9 cut(s) 39, 339, 1025, 2218, 2533, 2570, 3753, 4578, 5117
BssNAI GTATAC 1 cut(s) 1625
BssNI GRCGYC 1 cut(s) 1131
BssSI CACGAG 4 cut(s) 221, 1220, 1643, 3480
BssT1I CCWWGG 7 cut(s) 39, 1025, 2218, 2570, 3753, 4578, 5117
Bst1107I GTATAC 1 cut(s) 1625
Bst2BI CACGAG 4 cut(s) 221, 1220, 1643, 3480
Bst2UI CCWGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
Bst6I CTCTTC 7 cut(s) 324, 994, 2465, 3918, 4269, 5259, 5379
BstACI GRCGYC 1 cut(s) 1131
BstAPI GCANNNNNTGC 1 cut(s) 3467
BstAUI TGTACA 1 cut(s) 4195
BstBAI YACGTR 3 cut(s) 184, 1607, 5012
BstBI TTCGAA 1 cut(s) 3675
BstDSI CCRYGG 4 cut(s) 339, 1025, 2533, 3753
BstEII GGTNACC 1 cut(s) 1252
BstFNI CGCG 3 cut(s) 646, 894, 5209
BstH2I RGCGCY 2 cut(s) 3128, 4565
BstHHI GCGC 4 cut(s) 896, 2031, 3127, 4564
BstMCI CGRYCG 2 cut(s) 273, 519
BstMWI GCNNNNNNNGC 8 cut(s) 833, 1154, 2321, 2348, 2360, 3467, 3476, 3972
BstNI CCWGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
BstPI GGTNACC 1 cut(s) 1252
BstSCI CCNGG 9 cut(s) 153, 421, 1890, 2847, 3502, 3901, 4138, 4215, 5107
BstSFI CTRYAG 3 cut(s) 2817, 3195, 4788
BstSLI GKGCMC 2 cut(s) 255, 477
BstSNI TACGTA 1 cut(s) 1607
BstUI CGCG 3 cut(s) 646, 894, 5209
BstV2I GAAGAC 8 cut(s) 678, 969, 1668, 2045, 2205, 2801, 5017, 5337
BstX2I RGATCY 5 cut(s) 638, 1637, 2509, 3286, 4991
BstXI CCANNNNNNTGG 3 cut(s) 502, 1316, 2552
BstYI RGATCY 5 cut(s) 638, 1637, 2509, 3286, 4991
BstZ17I GTATAC 1 cut(s) 1625
Bsu15I ATCGAT 1 cut(s) 1299
Bsu36I CCTNAGG 1 cut(s) 1491
BsuI GTATCC 3 cut(s) 570, 2282, 3527
BsuTUI ATCGAT 1 cut(s) 1299
BtgI CCRYGG 4 cut(s) 339, 1025, 2533, 3753
BtrI CACGTC 2 cut(s) 160, 2225
BtsI GCAGTG 4 cut(s) 2109, 3306, 4164, 4446
BtsIMutI CAGTG 9 cut(s) 1403, 2109, 2832, 3306, 4090, 4164, 4446, 4848, 5229
BveI ACCTGC 2 cut(s) 3916, 4838
CaiI CAGNNNCTG 4 cut(s) 2048, 2399, 3262, 3449
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 4 cut(s) 896, 2031, 3127, 4564
Cfr10I RCCGGY 3 cut(s) 426, 1807, 4241
ClaI ATCGAT 1 cut(s) 1299
CseI GACGC 3 cut(s) 635, 1154, 5377
CsiI ACCWGGT 1 cut(s) 1890
CspAI ACCGGT 1 cut(s) 4241
DraI TTTAAA 2 cut(s) 1050, 2373
DraIII CACNNNGTG 3 cut(s) 226, 1409, 5297
EaeI YGGCCR 3 cut(s) 424, 4213, 5110
Eam1104I CTCTTC 7 cut(s) 324, 994, 2465, 3918, 4269, 5259, 5379
EarI CTCTTC 7 cut(s) 324, 994, 2465, 3918, 4269, 5259, 5379
Ecl136II GAGCTC 1 cut(s) 767
Eco105I TACGTA 1 cut(s) 1607
Eco130I CCWWGG 7 cut(s) 39, 1025, 2218, 2570, 3753, 4578, 5117
Eco24I GRGCYC 3 cut(s) 255, 477, 769
Eco31I GGTCTC 3 cut(s) 1086, 3775, 4763
Eco32I GATATC 3 cut(s) 1945, 2881, 5198
Eco47I GGWCC 6 cut(s) 1510, 1688, 2629, 3152, 3905, 4964
Eco47III AGCGCT 2 cut(s) 3126, 4563
Eco53kI GAGCTC 1 cut(s) 767
Eco57I CTGAAG 3 cut(s) 782, 2045, 3934
Eco72I CACGTG 1 cut(s) 184
Eco81I CCTNAGG 1 cut(s) 1491
Eco88I CYCGRG 1 cut(s) 575
Eco91I GGTNACC 1 cut(s) 1252
EcoICRI GAGCTC 1 cut(s) 767
EcoO109I RGGNCCY 1 cut(s) 251
EcoO65I GGTNACC 1 cut(s) 1252
EcoRI GAATTC 3 cut(s) 1320, 1936, 3580
EcoRII CCWGG 9 cut(s) 153, 421, 1890, 2847, 3502, 3901, 4138, 4215, 5107
EcoRV GATATC 3 cut(s) 1945, 2881, 5198
EcoT14I CCWWGG 7 cut(s) 39, 1025, 2218, 2570, 3753, 4578, 5117
EcoT22I ATGCAT 3 cut(s) 2193, 2437, 3823
EcoT38I GRGCYC 3 cut(s) 255, 477, 769
ErhI CCWWGG 7 cut(s) 39, 1025, 2218, 2570, 3753, 4578, 5117
Esp3I CGTCTC 4 cut(s) 996, 1138, 3036, 5360
FalI AAGNNNNNCTT 4 cut(s) 3258, 3290, 4990, 5022
FaqI GGGAC 6 cut(s) 896, 2527, 2588, 2615, 4006, 5170
FauI CCCGC 3 cut(s) 701, 2788, 4531
FbaI TGATCA 3 cut(s) 2905, 3157, 4045
FblI GTMKAC 4 cut(s) 273, 1287, 1624, 3652
FriOI GRGCYC 3 cut(s) 255, 477, 769
GlaI GCGC 4 cut(s) 895, 2030, 3126, 4563
GsuI CTGGAG 3 cut(s) 2738, 3379, 4017
HaeII RGCGCY 2 cut(s) 3128, 4565
HapII CCGG 4 cut(s) 352, 427, 1808, 4242
HgaI GACGC 3 cut(s) 635, 1154, 5377
HhaI GCGC 4 cut(s) 896, 2031, 3127, 4564
Hin1I GRCGYC 1 cut(s) 1131
Hin6I GCGC 4 cut(s) 894, 2029, 3125, 4562
HinP1I GCGC 4 cut(s) 894, 2029, 3125, 4562
HincII GTYRAC 4 cut(s) 274, 1915, 2107, 4095
HindII GTYRAC 4 cut(s) 274, 1915, 2107, 4095
HindIII AAGCTT 3 cut(s) 1758, 3216, 3946
HpaI GTTAAC 1 cut(s) 1915
HpaII CCGG 4 cut(s) 352, 427, 1808, 4242
Hpy99I CGWCG 7 cut(s) 314, 374, 449, 470, 473, 721, 5209
HpyF10VI GCNNNNNNNGC 8 cut(s) 833, 1154, 2321, 2348, 2360, 3467, 3476, 3972
Hsp92I GRCGYC 1 cut(s) 1131
HspAI GCGC 4 cut(s) 894, 2029, 3125, 4562
Ksp22I TGATCA 3 cut(s) 2905, 3157, 4045
KspAI GTTAAC 1 cut(s) 1915
LguI GCTCTTC 2 cut(s) 324, 3918
MabI ACCWGGT 1 cut(s) 1890
MbiI CCGCTC 1 cut(s) 404
MfeI CAATTG 5 cut(s) 918, 2207, 3882, 4146, 4547
MflI RGATCY 5 cut(s) 638, 1637, 2509, 3286, 4991
MhlI GDGCHC 4 cut(s) 255, 477, 769, 861
MlsI TGGCCA 2 cut(s) 4215, 5112
MluI ACGCGT 1 cut(s) 644
MluNI TGGCCA 2 cut(s) 4215, 5112
MlyI GAGTC 5 cut(s) 186, 539, 3338, 5221, 5276
MmeI TCCRAC 4 cut(s) 1213, 2533, 2682, 4387
Mox20I TGGCCA 2 cut(s) 4215, 5112
Mph1103I ATGCAT 3 cut(s) 2193, 2437, 3823
MroXI GAANNNNTTC 2 cut(s) 848, 3732
MscI TGGCCA 2 cut(s) 4215, 5112
MslI CAYNNNNRTG 4 cut(s) 2440, 3411, 4401, 5034
Msp20I TGGCCA 2 cut(s) 4215, 5112
MspA1I CMGCKG 4 cut(s) 1370, 2663, 2795, 3173
MspI CCGG 4 cut(s) 352, 427, 1808, 4242
MspR9I CCNGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
MunI CAATTG 5 cut(s) 918, 2207, 3882, 4146, 4547
MvaI CCWGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
MvnI CGCG 3 cut(s) 646, 894, 5209
MwoI GCNNNNNNNGC 8 cut(s) 833, 1154, 2321, 2348, 2360, 3467, 3476, 3972
NcoI CCATGG 2 cut(s) 1025, 3753
NheI GCTAGC 1 cut(s) 3176
NlaIV GGNNCC 9 cut(s) 6, 253, 475, 1690, 2143, 2631, 4134, 4852, 4956
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 5 cut(s) 1252, 2064, 4069, 4483, 5224
NsiI ATGCAT 3 cut(s) 2193, 2437, 3823
NspV TTCGAA 1 cut(s) 3675
OliI CACNNNNGTG 1 cut(s) 5034
PaeI GCATGC 2 cut(s) 3821, 4400
PagI TCATGA 1 cut(s) 1390
PciI ACATGT 4 cut(s) 191, 3020, 5020, 5340
PciSI GCTCTTC 2 cut(s) 324, 3918
PcsI WCGNNNNNNNCGW 2 cut(s) 525, 5008
PdmI GAANNNNTTC 2 cut(s) 848, 3732
PfeI GAWTC 8 cut(s) 266, 744, 1775, 2015, 2039, 2684, 4592, 5122
PflFI GACNNNGTC 2 cut(s) 368, 550
PflMI CCANNNNNTGG 6 cut(s) 1716, 2165, 2281, 3312, 4733, 4829
PfoI TCCNGGA 3 cut(s) 153, 3901, 4138
PinAI ACCGGT 1 cut(s) 4241
Ple19I CGATCG 1 cut(s) 519
PleI GAGTC 5 cut(s) 185, 538, 3337, 5221, 5275
PmaCI CACGTG 1 cut(s) 184
PmlI CACGTG 1 cut(s) 184
PpsI GAGTC 5 cut(s) 185, 538, 3337, 5221, 5275
Ppu21I YACGTR 3 cut(s) 184, 1607, 5012
PscI ACATGT 4 cut(s) 191, 3020, 5020, 5340
PsiI TTATAA 1 cut(s) 5282
Psp124BI GAGCTC 1 cut(s) 769
Psp6I CCWGG 9 cut(s) 153, 421, 1890, 2847, 3502, 3901, 4138, 4215, 5107
PspCI CACGTG 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 1252
PspGI CCWGG 9 cut(s) 153, 421, 1890, 2847, 3502, 3901, 4138, 4215, 5107
PspN4I GGNNCC 9 cut(s) 6, 253, 475, 1690, 2143, 2631, 4134, 4852, 4956
PspOMI GGGCCC 2 cut(s) 251, 473
PstI CTGCAG 1 cut(s) 4792
PstNI CAGNNNCTG 4 cut(s) 2048, 2399, 3262, 3449
PsuI RGATCY 5 cut(s) 638, 1637, 2509, 3286, 4991
PsyI GACNNNGTC 2 cut(s) 368, 550
PvuI CGATCG 1 cut(s) 519
PvuII CAGCTG 1 cut(s) 1370
RseI CAYNNNNRTG 4 cut(s) 2440, 3411, 4401, 5034
SacI GAGCTC 1 cut(s) 769
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 2 cut(s) 324, 3918
ScaI AGTACT 1 cut(s) 1656
SchI GAGTC 5 cut(s) 186, 539, 3338, 5221, 5276
ScrFI CCNGG 9 cut(s) 155, 423, 1892, 2849, 3504, 3903, 4140, 4217, 5109
SduI GDGCHC 4 cut(s) 255, 477, 769, 861
SexAI ACCWGGT 1 cut(s) 1890
SfcI CTRYAG 3 cut(s) 2817, 3195, 4788
SfuI TTCGAA 1 cut(s) 3675
SinI GGWCC 6 cut(s) 1510, 1688, 2629, 3152, 3905, 4964
SmiMI CAYNNNNRTG 4 cut(s) 2440, 3411, 4401, 5034
SmlI CTYRAG 9 cut(s) 128, 436, 656, 1552, 2558, 2981, 3317, 4319, 4557
SmoI CTYRAG 9 cut(s) 128, 436, 656, 1552, 2558, 2981, 3317, 4319, 4557
SnaBI TACGTA 1 cut(s) 1607
SpeI ACTAGT 2 cut(s) 2758, 4255
SphI GCATGC 2 cut(s) 3821, 4400
SspI AATATT 4 cut(s) 2338, 3672, 4891, 5311
SstI GAGCTC 1 cut(s) 769
StyD4I CCNGG 9 cut(s) 153, 421, 1890, 2847, 3502, 3901, 4138, 4215, 5107
StyI CCWWGG 7 cut(s) 39, 1025, 2218, 2570, 3753, 4578, 5117
TaqII GACCGA 1 cut(s) 259
TatI WGTACW 3 cut(s) 1654, 4195, 4411
TauI GCSGC 4 cut(s) 2366, 3695, 4796, 5096
TfiI GAWTC 8 cut(s) 266, 744, 1775, 2015, 2039, 2684, 4592, 5122
TscAI CASTG 9 cut(s) 1410, 2116, 2839, 3313, 4090, 4164, 4453, 4848, 5229
TseFI GTSAC 5 cut(s) 1252, 2064, 4069, 4483, 5224
Tsp45I GTSAC 5 cut(s) 1252, 2064, 4069, 4483, 5224
TspGWI ACGGA 6 cut(s) 158, 639, 999, 2522, 2755, 4947
TspRI CASTG 9 cut(s) 1410, 2116, 2839, 3313, 4090, 4164, 4453, 4848, 5229
Tth111I GACNNNGTC 2 cut(s) 368, 550
Van91I CCANNNNNTGG 6 cut(s) 1716, 2165, 2281, 3312, 4733, 4829
VpaK11BI GGWCC 6 cut(s) 1510, 1688, 2629, 3152, 3905, 4964
XapI RAATTY 6 cut(s) 866, 1320, 1612, 1936, 3580, 4645
XcmI CCANNNNNNNNNTGG 2 cut(s) 1520, 2698
XmaJI CCTAGG 2 cut(s) 2218, 2570
XmiI GTMKAC 4 cut(s) 273, 1287, 1624, 3652
XmnI GAANNNNTTC 2 cut(s) 848, 3732
ZraI GACGTC 1 cut(s) 1132
ZrmI AGTACT 1 cut(s) 1656
Zsp2I ATGCAT 3 cut(s) 2193, 2437, 3823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.