Rh4DG125900

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011973
Physical Location & Seq
Reverse (-)
20488163 .. 20504858
16696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG125900.1

Sequence Viewer

Length: 5310 bp
ATGAATACAGTTTTGCCTACCGTAATCGAGATTACCATTGAAACTCCAGATGACTCGCAAGTGACCCTCAAGGGTATCTCTACGGACAGGATCTTAGATGTGAGAAAGCTGTTGGGGGTCCACGTAGATACATGTCATTTCACCAATTTCTCCCTATCACACGAGGTGCGAGGACCCAGACTGAAAGATACTGTGGACATCCTCTCTCTCAAACCCTGCCACCTCACCATCGTCGAAGAGGACTACACGGAAGAGCAAGCCGTGGCTCACATTCGAAGACTGGTTGACATCGTCGCCTGCACGACGTCGTTTGGCTCGTCGTCTTCTTCACCCAGAACCCCCGGTTCTGCTTCGGTTCCGGCTCCGGTTCGGTCGAGTTCTAAAGAGTCCGGTTTAGACGAGGGCGACGAGCACAATGCCGTTCCGAAGACCAAAGTTTCGAGTCCGATTCCGATCGCCGGCGAGAAAGGCGGCGAGTCGGCAATGTATCCTCCGCCGAGGCTCGGCCAGTTTTACGACTTCTTCTCCTTGTCTCACCTCACTCCTCCACTCCACTACGTTAGAAGATCTAGCCGTCCGTTCCTTGAGGACAAGGCAGAGGAGGACCTGTTTCAAATTGATGTTCGGGTTTGTAGTGGGAAGCCGACGACTATAGTAGCATCCAGAAAAGGGTTCTACCCTGCTGGAAAACGTCCTCTTATTACTCACTCTCTGGTTGCTTTGCTTCAACAGATAAGCCGGCCGTTCGATGCTGCATACAATGCTGTCATGAAGGCCTTCACCGAGCACAATAAATTTGGGAACCTTCCTTATGGTTTTCGGGCAAACACATGGGTTGTCCCTCCGGTTGTTGCTGAGAACCCGTCTGTTTTCCCGCCACTTCCTCTAGAAGATGAAAACTGGGGAGGAAATGGGGGTGGGCAGGGAAGAGATGGTAAACACGATAACAGGCCATGGGCGAAAGAGTTTGCCATTCTTGCAGCAATGCCTTGTGCAACAGCAGAGGAGAGGGATGCACCTGATGCAAGTGTAAAGGTGGATTGCAAGAATGATGGTAGTCGAGTTCTAGGAATGCCACAAGAAGAAGTTACTCAGAGAAACCTACTCAAAGGCATAACAGCTGATGAGAGTGCAACTGTTCATGATACTTCCACACTAGGTGTTGTGGTTGTTAGGCATTGTGGATTTACTGCTGTTGTGAAAGTTTTAGCTGAGGTGAATTGGGTGGGGAAACCTGTTCCTCAGGACATTGAAATTGAGGACCAGCCTGAAGGAGGTGCAAATGCCTTGAATGTTAACAGCCTGAGAATGCTATTGCAACAGTCATCCCTCCTTCAGTCATCAACTACGGTTCAACGAAGCCAGAGCACAGATTTTGAAAATTTACGTTCTGCTAGGTCCTTAGTAAGGAAAGTACTCGAAGAAAGTTTATTAAGGTTGCAGGGTGGGCCATCTAATCACACCAAGTCGATCAGATGGGAGCTAGGAGCATGTTGGGTGCAACATTTGCAAAATCAGGCTTCAGCGAAAAATGAGCCTAAGAAAAATGAAGAAGCTAAGATAGAACCGGCTGTTAAGGGCCTCGGAAAGCAAGGTGGACTGCTAAAGGAAATAAAGAAGAAAATGGATATCAGGAGCAGTAAAACTGAACAGGGCAAGGAAGTTCTTGGGGGTAACAACCTTGATCTTAATAAGAATTCAGTCACCACCAGTCAGGAGGAGTTAGAAAAGCGAGATGCCGAAAAGGAAATAAATTGGAGAAGGCTACTTCCTGATGCATCCTATTCGCGGCTCAAAGAATCAGATACTGGTCTCCACCTTAAGTCACCTGATGAGTTGATTGAAATGGCACACAAGTATTACGCTGATACTGCACTTCCAAAACTGGTGGCAGATTTTGGCTCCCTTGAACTTTCACCAGTTGATGGAAGGACATTGACAGATTTTATGCATACAAGGGGTTTGAAAATGTGTTCCCTGGGACGCGTGGTTGAACTTGCAGATAAGCTCCCTCATGTACAATCACTCTGTATCCATGAGATGGTTGTGCGAGCATACAAACACATACTGCAAGCTGTTGTAGCAGCAGTTGATAATGTCGCAGACTTGGCTGCATCCATAGCAGCATGTCTAAATATTCTGCTAGGAACCCCATCACCTGAAAATGTGGATGGAGACATTACTTGTGAGGATTCGTTAAAATGGAAGTGGGTGGAAACATTCCTTTTGAAGAGGTTTGGATGGCAATGGAAACATGAAAGCGTTGAGGACTTTAGAAAGTTTGCCATTCTTCGTGGATTGTGCCATAAGGTTGGCCTTGAGCTTGTGCCAAGGGATTATGATATGGACACTTCATCTCCTTTTAGGAAATCAGATATAGTAAGCATGGTCCCTGTGTACAAGCATGTTGCATGTTCATCTGCTGATGGACGTACACTTTTGGAATCTTCCAAGACTTCCTTGGATAAAGGTAAACTGGAGGATGCTGTTAATTATGGCACTAAGGCGTTGTCAAAACTTGTATCAGTATGTGGGCCTTATCATCGAATGACCGCTGGAGCATATAGTCTTCTCGCTGTAGTACTGTATCACACTGGGGATTTTAATCAGGCAACCATCTATCAGCAAAAAGCATTGGATATTAATGAGAGAGAGCTTGGACTTGATCATCCTGATACAATGAAAAGTTACGGGGATTTAGCTGTGTTCTACTACCGACTTCAACATACAGAGTTGGCCTTGAAGTACGTCAATCGTGCGCTGTATCTTTTGCACCTAACATGTGGGCCGTCTCATCCAAACACGGCTGCCACCTATATCAATGTAGCAATGATGGAAGAAGGTTTGGGGAACGTCCATGTTGCTCTTAGGTACCTCCATGAAGCTCTCAAGTGCAATCAAAGGCTACTTGGAGCTGATCATATACAGACTGCGGCTAGCTATCATGCCATAGCTATCGCACTTTCTTTGATGGAGGCATACTCATTAAGTGTTCAGCACGAACAAACTACCTTGCAAATTCTGCAGGCGAAACTCGGGTCAGAGGATCTACGCACACAGGATGCGGCTGCATGGCTTGAGTATTTTGAGTCCAAAGCTTTGGAGCAGCAAGAAGCTGCACGGAATGGTACTCCAAAGCCAGATGCTTCAATCTCCAGCAAAGGTCATTTAAGTGTATCAGATCTTTTGGATTATATAACACCAGATGCTGATATGAAAGCTAGAGAGGCCCAAAGGAAAGCTCGTTTGAAGGTTAAAGGCAAACCAGGTCAGAACGGGGAGGCAGTCTCTGATGAGTACCAGAAGGATGAAAATTTGTTACCAAGTCATCCTGTTGCAGAGAATTTAAGTGATAAAGAGAACAAGTCTGAAGCACAATTAGGAGAACCTAGGAATGAGAAATATGATTCCCGTCTACTTGAGCAATCAATAATATATGCTACACATGATGATCTGGCACAAGATGACAACTCAGATGAAGGCTGGCAAGAGGCTGTTCCTAAAGGCCGGTCACTTATAGGTCGCAAATCTACTGGGTCAAGGAGGCCAAGCCTTGAAAAACTGAATACTAACTTTATCAATACATCCCAGTCATCAAGATATCGAGGCAAGGTCAATAATTTCACATCCCCCAAAACAAGCCCAAATGAGCCTGCTTCTTCAACTGGACCTGCTCCTCCAGTGTCAAAGAAGTTTGTCAAGAGTGCAAGCTTCAGTCCTAAGCAAAACAACTCTAGCACATCAGCTACCGGAGCTGCAGAGAGATTGCCAAACCCAAAGTCAGCACCATCTACCCCAGCTTCTGTTGATCAAGTCATCAAGTCTGTAAGTCCGATCAGTGTTCAGTCAACTGGAAAACTCTTTTCCTACAAAGAAGTTGCATTGGCTCCACCTGGGACAATTGTGAAGGCAGTGGCGGAGCAGTTACCAAAGCCGATTGTACAAGTTTCTCAGGTGGGCCAGGAGACACCTACAACAGAGGTCACTGTTGGTGAAGTGACGGCAATTAAAGATGTGAAGGATAAAAAAAATAAGAAATCGACAGGAGAGAAACAGGTTATGGTTTCTGAGAAAAGCCCTTCTGTAGTACGATCAGCAAAACTGAAGGATTCTGTGGCAAAGGTGTCCTTGGAAGTTGTGAAGCATGCTAGTGTTGGAGTACAAGTTGAAGCTGAATCTGAAGCTGTGGAATTAGAAAACACTGCTTTTGAAGGTGCAGAAGTTGAAAATGTAAAAGTTCCTGTTCCTAGCGTTGAGATTTCTGATGCTTCCCAAAGTCCAAATACCACAGCTGCTGAATGTGGACCATCAGACCCCCTAGGGCCAGACAGTTGCCCCAGGACTTCCTCTGATTCAGAACCTCCATCTGTTTTAACTGAGACTAGAACCAACAATCCTTCTAATACAGGGGAGCGAAAGTCACGTGATTTACCCAATGGCGACATAGTTAAACCAGTGCCAACTGGTGGGGAAAAGGTAGATGAACAAGAAACTGGAAAGGAGACGAGCAAAAAACTTTCTGCAGCCGCACCTCCTTTTAATCCATCCCCCATTCCAGTTTTTGGCTCAATTCCGGTTCCAGTTCCAGGTCTCAAAGATCATGGAGGGATTTTGCCCCCACCTGTAAATATTCCTCCGATGCTTGCAGTTAATCCTGTCCGTAGATCACCCCATCAGTCAGCGACAGCAAGAGTTCCTTATGGTCCACGACTTTCTGGTGGCTATAACAGATCTGGAAGTCGGGTTTCACGTAATAAACATAGCTTTCACAATGGTGAGCACACCGGGGATGGTCCTCCCAGAATAATGAACCCACATGCAGCTGAGTTTGTACCTGGCCAACCTTGGGTTCAAAATGGCTATCCACTGTCTCCCAATGGTTTCTTGCCTTCCCCAAATGGTTATCCAGTGTCCCCAAATGGATATCCGGTATCACCGAATGGTACTCCGGTGAATCAAAACGGGTCTCCGACATCTCCAGTCAGTTCGGATGAGTCATCACCAGTTGTGACTGCTGATATTGGTGTTGGAGCAAATACTGAAGGTGCAGGGAAGGAGACTAATGATAGGTTATCAGTACAAGTTGAATGTGACAAGCAACCAATTGAAGGAGAGCTGCAGGAAGAACAGTCTTTAGATGATGTAAACGTTCCTCCTGAAACTGAAGAAAAGCCTATTGATACCGATACAGTTCCTGGTGATACTATTGTGGAAAAGGAGACTAGCAATCTAGTAGTTGAGGAAAAACCAAGTAAATGTTGGGGAGATTATAGCGATAATGAAGCTGAGGTCATTGAGGTTAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1769

Amino Acids

192.4

Weight (kDa)

5.81

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 28 - 98 3e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 638 - 781 6e-23 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 822 - 883 2e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 822 - 921 2.7e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 851 - 921 1.4e-12 Tetratricopeptide repeat
TPR_10 PF13374 851 - 892 2e-09 Tetratricopeptide repeat
TPR_12 PF13424 893 - 959 8.3e-08 Tetratricopeptide repeat
TPR_12 PF13424 935 - 1009 1.9e-10 Tetratricopeptide repeat
TPR_10 PF13374 935 - 975 4e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 3679
Acc65I GGTACC 1 cut(s) 2871
AccB1I GGYRCC 1 cut(s) 2871
AccB7I CCANNNNNTGG 4 cut(s) 1925, 2041, 4466, 4562
AccI GTMKAC 1 cut(s) 3415
AccII CGCG 2 cut(s) 1789, 1986
AciI CCGC 9 cut(s) 471, 494, 875, 1789, 2553, 2933, 3065, 3917, 4527
AclI AACGTT 1 cut(s) 5148
AclWI GGATC 2 cut(s) 98, 3054
AcoI YGGCCR 3 cut(s) 505, 740, 4837
AcsI RAATTY 6 cut(s) 794, 1381, 1696, 3018, 3313, 3343
AcuI CTGAAG 9 cut(s) 1290, 1319, 1506, 3390, 3697, 4124, 4200, 5061, 5184
AcvI CACGTG 1 cut(s) 4424
AcyI GRCGYC 1 cut(s) 305
AdeI CACNNNGTG 2 cut(s) 166, 1160
AflII CTTAAG 1 cut(s) 1820
AflIII ACRYGT 3 cut(s) 131, 1984, 2780
AjnI CCWGG 8 cut(s) 1977, 3265, 3892, 3960, 4337, 4585, 4834, 5194
AjuI GAANNNNNNNTTGG 2 cut(s) 4833, 4865
AleI CACNNNNGTG 1 cut(s) 4773
AloI GAACNNNNNNTCC 2 cut(s) 4378, 4410
Alw21I GWGCWC 4 cut(s) 414, 789, 1370, 4782
AlwI GGATC 2 cut(s) 98, 3054
AlwNI CAGNNNCTG 6 cut(s) 1808, 3209, 3290, 3803, 4295, 5195
Ama87I CYCGRG 1 cut(s) 3035
ApoI RAATTY 6 cut(s) 794, 1381, 1696, 3018, 3313, 3343
ArsI GACNNNNNNTTYG 6 cut(s) 421, 453, 3023, 3055, 3764, 3796
AseI ATTAAT 1 cut(s) 2643
Asp700I GAANNNNTTC 1 cut(s) 776
Asp718I GGTACC 1 cut(s) 2871
AspA2I CCTAGG 2 cut(s) 3389, 4318
AspLEI GCGC 1 cut(s) 2761
AsuC2I CCSGG 2 cut(s) 342, 4786
AsuII TTCGAA 1 cut(s) 274
AsuNHI GCTAGC 1 cut(s) 2936
AvaI CYCGRG 1 cut(s) 3035
AvrII CCTAGG 2 cut(s) 3389, 4318
AxyI CCTNAGG 1 cut(s) 1242
BaeI ACNNNNGTAYC 4 cut(s) 4935, 4968, 5193, 5226
BalI TGGCCA 1 cut(s) 4839
BanI GGYRCC 1 cut(s) 2871
BauI CACGAG 1 cut(s) 161
BbrPI CACGTG 1 cut(s) 4424
BbsI GAAGAC 4 cut(s) 283, 315, 434, 2561
Bbv12I GWGCWC 4 cut(s) 414, 789, 1370, 4782
BbvCI CCTCAGC 2 cut(s) 1212, 5286
BceAI ACGGC 7 cut(s) 245, 404, 558, 727, 2773, 2820, 4017
BcgI CGANNNNNNTGC 4 cut(s) 398, 432, 1767, 1801
BciT130I CCWGG 8 cut(s) 1979, 3267, 3894, 3962, 4339, 4587, 4836, 5196
BciVI GTATCC 2 cut(s) 498, 2042
BclI TGATCA 3 cut(s) 2665, 2917, 3808
BcnI CCSGG 2 cut(s) 342, 4786
BfmI CTRYAG 7 cut(s) 651, 2577, 3023, 3756, 4083, 4521, 5117
BfrI CTTAAG 1 cut(s) 1820
BfuAI ACCTGC 1 cut(s) 3679
BfuI GTATCC 2 cut(s) 498, 2042
BglII AGATCT 3 cut(s) 566, 3181, 4730
BlnI CCTAGG 2 cut(s) 3389, 4318
BmcAI AGTACT 2 cut(s) 1416, 2583
BmeT110I CYCGRG 1 cut(s) 3035
BmrI ACTGGG 4 cut(s) 910, 2604, 3543, 3583
BmtI GCTAGC 1 cut(s) 2940
BmuI ACTGGG 4 cut(s) 910, 2604, 3543, 3583
BpiI GAAGAC 4 cut(s) 283, 315, 434, 2561
BplI GAGNNNNNCTC 4 cut(s) 2966, 2998, 3272, 3304
BpmI CTGGAG 6 cut(s) 30, 2498, 2577, 3139, 3663, 4962
Bpu10I CCTNAGC 3 cut(s) 1212, 3720, 5286
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 6 cut(s) 53, 605, 2339, 2873, 3098, 3440
BpuMI CCSGG 2 cut(s) 342, 4786
BsaAI YACGTR 3 cut(s) 124, 4424, 4751
BsaBI GATNNNNATC 2 cut(s) 452, 2604
BsaHI GRCGYC 1 cut(s) 305
BsaI GGTCTC 3 cut(s) 1817, 4595, 4971
BsaWI WCCGGW 7 cut(s) 364, 389, 844, 3749, 4573, 4927, 4948
Bse118I RCCGGY 4 cut(s) 458, 738, 1567, 3507
Bse21I CCTNAGG 1 cut(s) 1242
Bse3DI GCAATG 4 cut(s) 489, 990, 2252, 2835
Bse8I GATNNNNATC 2 cut(s) 452, 2604
BseBI CCWGG 8 cut(s) 1979, 3267, 3894, 3962, 4339, 4587, 4836, 5196
BseJI GATNNNNATC 2 cut(s) 452, 2604
BseMI GCAATG 4 cut(s) 489, 990, 2252, 2835
BseRI GAGGAG 5 cut(s) 534, 614, 1019, 1733, 3666
BseX3I CGGCCG 1 cut(s) 740
BseYI CCCAGC 1 cut(s) 3796
BsgI GTGCAG 5 cut(s) 283, 1857, 3102, 4236, 5067
Bsh1236I CGCG 2 cut(s) 1789, 1986
Bsh1285I CGRYCG 3 cut(s) 374, 456, 743
BshNI GGYRCC 1 cut(s) 2871
BsiEI CGRYCG 3 cut(s) 374, 456, 743
BsiHKAI GWGCWC 4 cut(s) 414, 789, 1370, 4782
BsiHKCI CYCGRG 1 cut(s) 3035
BslFI GGGAC 5 cut(s) 824, 1995, 2375, 3910, 4897
BsmBI CGTCTC 2 cut(s) 2796, 4496
BsmFI GGGAC 5 cut(s) 824, 1995, 2375, 3910, 4897
BsmI GAATGC 2 cut(s) 1077, 1314
Bso31I GGTCTC 3 cut(s) 1817, 4595, 4971
BsoBI CYCGRG 1 cut(s) 3035
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 4 cut(s) 414, 789, 1370, 4782
Bsp1407I TGTACA 3 cut(s) 2017, 2397, 3940
Bsp19I CCATGG 1 cut(s) 953
BspACI CCGC 9 cut(s) 471, 494, 875, 1789, 2553, 2933, 3065, 3917, 4527
BspFNI CGCG 2 cut(s) 1789, 1986
BspHI TCATGA 2 cut(s) 768, 1141
BspMAI CTGCAG 4 cut(s) 3027, 3760, 4525, 5121
BspMI ACCTGC 1 cut(s) 3679
BspOI GCTAGC 1 cut(s) 2940
BspPI GGATC 2 cut(s) 98, 3054
BspQI GCTCTTC 1 cut(s) 246
BspT104I TTCGAA 1 cut(s) 274
BspT107I GGYRCC 1 cut(s) 2871
BspTI CTTAAG 1 cut(s) 1820
BspTNI GGTCTC 3 cut(s) 1817, 4595, 4971
BsrDI GCAATG 4 cut(s) 489, 990, 2252, 2835
BsrFI RCCGGY 4 cut(s) 458, 738, 1567, 3507
BsrGI TGTACA 3 cut(s) 2017, 2397, 3940
BssAI RCCGGY 4 cut(s) 458, 738, 1567, 3507
BssNI GRCGYC 1 cut(s) 305
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 7 cut(s) 953, 2330, 2460, 3389, 4128, 4318, 4844
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 8 cut(s) 1979, 3267, 3894, 3962, 4339, 4587, 4836, 5196
Bst6I CTCTTC 4 cut(s) 231, 246, 922, 2225
BstACI GRCGYC 1 cut(s) 305
BstAFI CTTAAG 1 cut(s) 1820
BstAPI GCANNNNNTGC 4 cut(s) 761, 1022, 1507, 3022
BstAUI TGTACA 3 cut(s) 2017, 2397, 3940
BstBAI YACGTR 3 cut(s) 124, 4424, 4751
BstBI TTCGAA 1 cut(s) 274
BstDSI CCRYGG 2 cut(s) 261, 953
BstENI CCTNNNNNAGG 2 cut(s) 1272, 1405
BstFNI CGCG 2 cut(s) 1789, 1986
BstHHI GCGC 1 cut(s) 2761
BstMCI CGRYCG 3 cut(s) 374, 456, 743
BstNI CCWGG 8 cut(s) 1979, 3267, 3894, 3962, 4339, 4587, 4836, 5196
BstNSI RCATGY 8 cut(s) 135, 1494, 2130, 2408, 2415, 2784, 4148, 4820
BstSFI CTRYAG 7 cut(s) 651, 2577, 3023, 3756, 4083, 4521, 5117
BstUI CGCG 2 cut(s) 1789, 1986
BstV2I GAAGAC 4 cut(s) 283, 315, 434, 2561
BstX2I RGATCY 5 cut(s) 90, 566, 3046, 3181, 4730
BstXI CCANNNNNNTGG 2 cut(s) 2312, 3100
BstYI RGATCY 5 cut(s) 90, 566, 3046, 3181, 4730
BstZI CGGCCG 1 cut(s) 740
Bsu36I CCTNAGG 1 cut(s) 1242
BsuI GTATCC 2 cut(s) 498, 2042
BtgI CCRYGG 2 cut(s) 261, 953
BtsI GCAGTG 2 cut(s) 3918, 4200
BtsIMutI CAGTG 9 cut(s) 2592, 3687, 3844, 3918, 3984, 4200, 4461, 4865, 4914
BveI ACCTGC 1 cut(s) 3679
CaiI CAGNNNCTG 6 cut(s) 1808, 3209, 3290, 3803, 4295, 5195
CciI TCATGA 2 cut(s) 768, 1141
CfoI GCGC 1 cut(s) 2761
Cfr10I RCCGGY 4 cut(s) 458, 738, 1567, 3507
CseI GACGC 1 cut(s) 1992
CsiI ACCWGGT 1 cut(s) 3265
CspCI CAANNNNNGTGG 2 cut(s) 1869, 1904
DraIII CACNNNGTG 2 cut(s) 166, 1160
EaeI YGGCCR 3 cut(s) 505, 740, 4837
EagI CGGCCG 1 cut(s) 740
Eam1104I CTCTTC 4 cut(s) 231, 246, 922, 2225
EarI CTCTTC 4 cut(s) 231, 246, 922, 2225
EciI GGCGGA 2 cut(s) 483, 3932
EclXI CGGCCG 1 cut(s) 740
Eco130I CCWWGG 7 cut(s) 953, 2330, 2460, 3389, 4128, 4318, 4844
Eco147I AGGCCT 1 cut(s) 776
Eco31I GGTCTC 3 cut(s) 1817, 4595, 4971
Eco32I GATATC 3 cut(s) 1630, 3602, 4925
Eco52I CGGCCG 1 cut(s) 740
Eco57I CTGAAG 9 cut(s) 1290, 1319, 1506, 3390, 3697, 4124, 4200, 5061, 5184
Eco72I CACGTG 1 cut(s) 4424
Eco81I CCTNAGG 1 cut(s) 1242
Eco88I CYCGRG 1 cut(s) 3035
EcoNI CCTNNNNNAGG 2 cut(s) 1272, 1405
EcoO109I RGGNCCY 4 cut(s) 173, 604, 1398, 1579
EcoRI GAATTC 1 cut(s) 1696
EcoRII CCWGG 8 cut(s) 1977, 3265, 3892, 3960, 4337, 4585, 4834, 5194
EcoRV GATATC 3 cut(s) 1630, 3602, 4925
EcoT14I CCWWGG 7 cut(s) 953, 2330, 2460, 3389, 4128, 4318, 4844
EcoT22I ATGCAT 2 cut(s) 1780, 1953
ErhI CCWWGG 7 cut(s) 953, 2330, 2460, 3389, 4128, 4318, 4844
Esp3I CGTCTC 2 cut(s) 2796, 4496
FalI AAGNNNNNCTT 6 cut(s) 2444, 2476, 4112, 4144, 4681, 4713
FaqI GGGAC 5 cut(s) 824, 1995, 2375, 3910, 4897
FauI CCCGC 1 cut(s) 882
FbaI TGATCA 3 cut(s) 2665, 2917, 3808
FblI GTMKAC 1 cut(s) 3415
GlaI GCGC 1 cut(s) 2760
GsaI CCCAGC 1 cut(s) 3800
GsuI CTGGAG 6 cut(s) 30, 2498, 2577, 3139, 3663, 4962
HgaI GACGC 1 cut(s) 1992
HhaI GCGC 1 cut(s) 2761
Hin1I GRCGYC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 2759
HinP1I GCGC 1 cut(s) 2759
HincII GTYRAC 3 cut(s) 286, 1297, 3849
HindII GTYRAC 3 cut(s) 286, 1297, 3849
HindIII AAGCTT 2 cut(s) 3096, 3709
HpaI GTTAAC 1 cut(s) 1297
Hpy99I CGWCG 7 cut(s) 236, 296, 307, 310, 322, 410, 649
Hsp92I GRCGYC 1 cut(s) 305
HspAI GCGC 1 cut(s) 2759
KpnI GGTACC 1 cut(s) 2875
KroI GCCGGC 2 cut(s) 458, 738
KroNI GCCGGC 2 cut(s) 460, 740
Ksp22I TGATCA 3 cut(s) 2665, 2917, 3808
KspAI GTTAAC 1 cut(s) 1297
LguI GCTCTTC 1 cut(s) 246
MabI ACCWGGT 1 cut(s) 3265
MfeI CAATTG 2 cut(s) 3900, 5103
MflI RGATCY 5 cut(s) 90, 566, 3046, 3181, 4730
MhlI GDGCHC 4 cut(s) 414, 789, 1370, 4782
MlsI TGGCCA 1 cut(s) 4839
MluI ACGCGT 1 cut(s) 1984
MluNI TGGCCA 1 cut(s) 4839
MlyI GAGTC 6 cut(s) 47, 395, 451, 485, 3098, 5003
MmeI TCCRAC 3 cut(s) 4135, 4994, 5008
Mox20I TGGCCA 1 cut(s) 4839
Mph1103I ATGCAT 2 cut(s) 1780, 1953
MreI CGCCGGCG 1 cut(s) 458
MroNI GCCGGC 2 cut(s) 458, 738
MroXI GAANNNNTTC 1 cut(s) 776
MscI TGGCCA 1 cut(s) 4839
MslI CAYNNNNRTG 3 cut(s) 3171, 4149, 4773
Msp20I TGGCCA 1 cut(s) 4839
MspA1I CMGCKG 4 cut(s) 1121, 2555, 4292, 4823
MspCI CTTAAG 1 cut(s) 1820
MunI CAATTG 2 cut(s) 3900, 5103
Mva1269I GAATGC 2 cut(s) 1077, 1314
MvaI CCWGG 8 cut(s) 1979, 3267, 3894, 3962, 4339, 4587, 4836, 5196
MvnI CGCG 2 cut(s) 1789, 1986
NaeI GCCGGC 2 cut(s) 460, 740
NciI CCSGG 2 cut(s) 342, 4786
NcoI CCATGG 1 cut(s) 953
NgoMIV GCCGGC 2 cut(s) 458, 738
NheI GCTAGC 1 cut(s) 2936
NmeAIII GCCGAG 2 cut(s) 483, 522
NmuCI GTSAC 9 cut(s) 61, 1702, 1824, 3510, 3982, 3997, 4419, 5008, 5090
NsiI ATGCAT 2 cut(s) 1780, 1953
NspI RCATGY 8 cut(s) 135, 1494, 2130, 2408, 2415, 2784, 4148, 4820
NspV TTCGAA 1 cut(s) 274
OliI CACNNNNGTG 1 cut(s) 4773
PaeI GCATGC 1 cut(s) 4148
PagI TCATGA 2 cut(s) 768, 1141
PasI CCCWGGG 1 cut(s) 1978
PceI AGGCCT 1 cut(s) 776
PciI ACATGT 2 cut(s) 131, 2780
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 3 cut(s) 314, 405, 4747
PctI GAATGC 2 cut(s) 1077, 1314
PdiI GCCGGC 2 cut(s) 460, 740
PdmI GAANNNNTTC 1 cut(s) 776
PfeI GAWTC 9 cut(s) 448, 1799, 2192, 2444, 3407, 4109, 4175, 4352, 4954
PflFI GACNNNGTC 1 cut(s) 290
PflMI CCANNNNNTGG 4 cut(s) 1925, 2041, 4466, 4562
Ple19I CGATCG 1 cut(s) 456
PleI GAGTC 6 cut(s) 47, 394, 450, 484, 3097, 5002
PmaCI CACGTG 1 cut(s) 4424
PmlI CACGTG 1 cut(s) 4424
PpsI GAGTC 6 cut(s) 47, 394, 450, 484, 3097, 5002
Ppu21I YACGTR 3 cut(s) 124, 4424, 4751
PpuMI RGGWCCY 3 cut(s) 173, 604, 1398
PscI ACATGT 2 cut(s) 131, 2780
PshBI ATTAAT 1 cut(s) 2643
Psp1406I AACGTT 1 cut(s) 5148
Psp5II RGGWCCY 3 cut(s) 173, 604, 1398
Psp6I CCWGG 8 cut(s) 1977, 3265, 3892, 3960, 4337, 4585, 4834, 5194
PspCI CACGTG 1 cut(s) 4424
PspFI CCCAGC 1 cut(s) 3796
PspGI CCWGG 8 cut(s) 1977, 3265, 3892, 3960, 4337, 4585, 4834, 5194
PspPPI RGGWCCY 3 cut(s) 173, 604, 1398
PstI CTGCAG 4 cut(s) 3027, 3760, 4525, 5121
PstNI CAGNNNCTG 6 cut(s) 1808, 3209, 3290, 3803, 4295, 5195
PsuI RGATCY 5 cut(s) 90, 566, 3046, 3181, 4730
PsyI GACNNNGTC 1 cut(s) 290
PvuI CGATCG 1 cut(s) 456
PvuII CAGCTG 3 cut(s) 1121, 4292, 4823
RseI CAYNNNNRTG 3 cut(s) 3171, 4149, 4773
SapI GCTCTTC 1 cut(s) 246
ScaI AGTACT 2 cut(s) 1416, 2583
SchI GAGTC 6 cut(s) 47, 395, 451, 485, 3098, 5003
SduI GDGCHC 4 cut(s) 414, 789, 1370, 4782
SexAI ACCWGGT 1 cut(s) 3265
SfcI CTRYAG 7 cut(s) 651, 2577, 3023, 3756, 4083, 4521, 5117
SfuI TTCGAA 1 cut(s) 274
SgrAI CRCCGGYG 1 cut(s) 458
SmiMI CAYNNNNRTG 3 cut(s) 3171, 4149, 4773
SmlI CTYRAG 7 cut(s) 68, 584, 1820, 2318, 2888, 3077, 3419
SmoI CTYRAG 7 cut(s) 68, 584, 1820, 2318, 2888, 3077, 3419
SphI GCATGC 1 cut(s) 4148
SseBI AGGCCT 1 cut(s) 776
SsiI CCGC 9 cut(s) 471, 494, 875, 1789, 2553, 2933, 3065, 3917, 4527
SspI AATATT 2 cut(s) 2137, 4630
StuI AGGCCT 1 cut(s) 776
StyI CCWWGG 7 cut(s) 953, 2330, 2460, 3389, 4128, 4318, 4844
TaqII GACCGA 1 cut(s) 360
TatI WGTACW 7 cut(s) 1414, 2017, 2397, 2581, 3940, 4159, 5077
TauI GCSGC 5 cut(s) 474, 1792, 2936, 3068, 4529
TfiI GAWTC 9 cut(s) 448, 1799, 2192, 2444, 3407, 4109, 4175, 4352, 4954
TscAI CASTG 9 cut(s) 2599, 3687, 3844, 3918, 3991, 4207, 4461, 4872, 4914
TseFI GTSAC 9 cut(s) 61, 1702, 1824, 3510, 3982, 3997, 4419, 5008, 5090
Tsp45I GTSAC 9 cut(s) 61, 1702, 1824, 3510, 3982, 3997, 4419, 5008, 5090
TspGWI ACGGA 5 cut(s) 98, 263, 567, 3136, 4649
TspRI CASTG 9 cut(s) 2599, 3687, 3844, 3918, 3991, 4207, 4461, 4872, 4914
Tth111I GACNNNGTC 1 cut(s) 290
Van91I CCANNNNNTGG 4 cut(s) 1925, 2041, 4466, 4562
Vha464I CTTAAG 1 cut(s) 1820
VspI ATTAAT 1 cut(s) 2643
XagI CCTNNNNNAGG 2 cut(s) 1272, 1405
XapI RAATTY 6 cut(s) 794, 1381, 1696, 3018, 3313, 3343
XbaI TCTAGA 1 cut(s) 886
XceI RCATGY 8 cut(s) 135, 1494, 2130, 2408, 2415, 2784, 4148, 4820
XcmI CCANNNNNNNNNTGG 2 cut(s) 2458, 5256
XmaJI CCTAGG 2 cut(s) 3389, 4318
XmiI GTMKAC 1 cut(s) 3415
XmnI GAANNNNTTC 1 cut(s) 776
ZraI GACGTC 1 cut(s) 306
ZrmI AGTACT 2 cut(s) 1416, 2583
Zsp2I ATGCAT 2 cut(s) 1780, 1953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.