RchiOBHm_Chr4g0405441

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011973
Physical Location & Seq
Reverse (-)
25972070 .. 25989329
17260 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37693

Sequence Viewer

Length: 5547 bp
ATGGCTCCCAAAACAGGGAAAGCCAAGCCACACAAATCCAAGGGAGACAAGAAGAAGAAAGAAGAGAAAGTTTTGCCTACCGTAATCGAGATTACCATTGAAACGCCAGATGAGTCGCAAGTGACCCTCAAGGGTATCTCTACGGACAGGATCTTAGATGTGAGAAAGCTGTTGGGGGTCCACGTAGATACATGTCATTTCACCAATTTCTCCCTATCACACGAGGTGCGAGGACCCAGACTGAAAGATACTGTGGACATCCTCTCTCTCAAACCCTGCCACCTCACCATCGTCGAAGAGGACTACACGGAAGAGCAAGCCGTGGCTCACATTCGAAGACTGGTTGACATCGTCGCCTGCACGACGTCGTTTGGCTCGTCGTCTTCTTCACCCAGAACCCCCGGTTCTGCTTCGGTTCCGGCTCCGGTTCGGTCGAGTTCTAAAGAGTCCGGTTTAGACGAGGGCGACGAGCACAATGCCGTTCCGAAGACCAAAGTTTCGAGTCCGATTCCGATCGCCGGCGAGAAAGGCGGCGAGTCGGCAATGTATCCTCCGCCGAGGCTCGGCCAGTTTTACGACTTCTTCTCCTTGTCTCACCTCACTCCTCCACTCCACTACGTTAGAAGATCTAGCCGTCCGTTCCTTGAGGACAAGGCAGAGGAGGACCTGTTTCAAATTGATGTTCGGGTTTGTAGTGGGAAGCCGACGACTATAGTAGCATCCAGAAAAGGGTTCTACCCTGCTGGAAAACGTCCTCTTATTACTCACTCTCTGGTTGCTTTGCTTCAACAGATAAGCCGGCCGTTCGATGCTGCGTACAATGCTGTCAAGAAGGCTTTCACCGAGCACAATAAATTTGGGAACCTTCCTTATGGTTTTCGGGCAAACACATGGGTTGTCCCTCCAGTTGTTGCTGAGAACCCGTCTGTTTTCCCGCCACTTCCTCTAGAAGATGAAAATTGGGGAGGAAATGGGGGTGGGCAGGGAAGAGACGGTAAACACGATAACAGGCCATGGGCGAAAGAGTTTGCCATTCTTGCAGCAATGCCTTGTGCAACAGCAGAGGAGAGGCAAGTTCGAGATCGAAAGGCCTTTCTGCTTCACAGTTTATTTGTCGACATCTCAGTTCTTAAAGCTGTTGCAGCTATTAAGAGCCTAATTGACACTCATCAATCTTGCCTAAATGATTCCACTCTTTCACTTCATCGTGAGGAAAAAATTGGAGATTTAATCATTAAGATCGTTAGAGATGCACCTGATGCAAGTGTAAAGGTGGATTGCAAGAATGATGGTAGTCGAGTTCTAGGAATGCCACAAGAAGAAGTTGCTCAAAGAAACCTACTGAAAGGCATAACAGCTGATGAGAGTGCAACTGTTCATGATACTTCTACACTAGGTGTTGTGGTTGTTAGGCATTGTGGATTTACTGCTGTTGTGAAAGTTTTAGCTGAGGTGAATTGGGTGGGGAAACCTGTTCCTCAGGACATTGAAATTGAGGACCAGCCTGAAGGAGGTGCAAATGCCTTGAATGTTAACAGCCTGAGAATGCTATTGCAACAGTCATCCCTCCTTCAGTCATCAACAACGGTTCAACGAAGCCAGAGTACAGATTTTGAAAATTTACGTTCTGCTAGGTCCTTAGTAAGGAAAGTACTCGAAGAAAGTTTATTAAGGTTGCAGGGTGGGCCATCTAATCACACCAAGTCGATCAGATGGGAGCTAGGAGCATGTTGGGTGCAACATTTGCAAAATCAGGCTTCAGCGAAAAATGAGCCTAAGAAAAATGAAGAAGCTAAGATAGAACCGGCTGTTAAGGGCCTCGGAAAGCAAGGTGGACTGCTAAAGGAAATAAAGAAGAAAATGGATATCAGGAGCAGTAAAACTGAACAGGGCAAGGAAGTTCTTGGGGGTAACAACCTTGATCTTAATAAGAATTCAGTCACCACCAGTCAGGAGGAGTTAGAAAAGCGAGATGCCGAAAAGGAAATAAATTGGAGAAGGCTACTTCCTGATGCATCCTATTCGCGGCTCAAAGAATCAGATACTGGTCTCCACCTTAAGTCACCTGATGAGTTGATTGAAATGGCACACAAGTATTACGCTGATACTGCACTTCCAAAACTGGTGGCAGATTTTGGCTCCCTTGAACTTTCACCAGTTGATGGAAGGACATTGACAGATTTTATGCATACAAGGGGTTTGCAAATGTGTTCCCTGGGACGCGTGGTTGAACTTGCAGATAAGCTCCCTCATGTACAATCACTCTGTATCCATGAGATGGTTGTGCGAGCATACAAACACATACTGCAAGCTGTTGTAGCAGCAGTTGATAATGTCGCAGACTTGGCTGCATCCATAGCAGCATGTCTAAATATTCTGCTAGGAACCCCATCACCTGAAAATGTGGATGGAGACATTACTTGTGAGGATACGTTAAAATGGAAGTGGGTGGAAACATTCCTTTTGAAGAGGTTTGGATGGCAATGGAAACATGAAAGCGTTGAGGACTTTAGAAAGTTTGCCATTCTTCGTGGATTGTGCCATAAGGTTGGCCTTGAGCTTGTGCCAAGGGACTATGATATGGACACTTCGTCTCCTTTTAGGAAATCAGATATAGTAAGCATGGTCCCTGTGTACAAGCATGTTGCATGTTCATCTGCTGATGGACGTACACTTTTGGAATCTTCCAAGACTTCCTTGGATAAAGGTAAACTGGAGGATGCTGTTAATTATGGCACTAAGGCGTTGTCAAAACTTGTATCAGTATGTGGGCCTTATCATCGAATGACTGCTGGAGCATATAGTCTTCTCGCTGTAGTACTGTATCACACTGGGGATTTTAATCAGGCAACCATCTATCAGCAAAAAGCATTGGATATTAATGAGAGAGAGCTTGGACTTGATCATCCTGATACAATGAAAAGTTACGGGGATTTAGCTGTGTTCTACTACCGACTTCAACATACAGAGTTGGCCTTGAAGTACGTCAATCGTGCACTGTATCTTTTGCACCTAACATGTGGGCCGTCTCATCCAAACACGGCTGCCACCTATATCAATGTAGCAATGATGGAAGAAGGTTTGGGGAACGTCCATGTTGCTCTTAGGTACCTCCATGAAGCTCTCAAGTGCAATCAAAGGCTACTTGGAGCTGATCATATACAGACTGCTGCTAGCTATCATGCCATAGCTATCGCACTTTCTTTGATGGAGGCATACTCATTAAGTGTTCAGCACGAACAAACTACCTTGCAAATTTTGCAGGCGAAACTCGGGTCAGAGGATCTGCGCACACAGGATGCGGCTGCATGGCTCGAGTATTTTGAGTCCAAAGCTTTGGAGCAGCAAGAAGCTGCACGGAATGGTACTCCAAAGCCAGATGCTTCAATCTCCAGCAAAGGTCATTTAAGTGTATCAGATCTTTTGGATTATATAACACCAGATGCTGATATGAAAGCTAGAGAGGCCCAAAGGAAAGCTCGTTTGAAGGTTAAAGGCAAACCAGGTCAGAATGGGGAGGCAGTCTCTGATGAGTACCAGAAGGATGAAAATTTGTTACCAAGTCATCCTGTTGCAGAGAATTTAAGTGATAAAGAGAACAAGTCTGAAGCACAATTAGGAGAACCTAGGAATGAGAAATATGATTCCCGTCTACTTGAGCAATCAATAATATTTGCTACACATGATGATCTGGCACAAGATGACAACTCAGATGAAGGCTGGCAAGAGGCTGTTCCTAAAGGCCGGTCACTTATAGGTCGCAAATCTACTGGGTCAAGGAGGCCAAGCCTTGAAAAACTGAATACTAACTTTATCAATACATCCCAGTCATCAAGATATCGAGGCAAGGCCAATAATTTCACATCCCCCAAAACAAGCCCAAATGAACCTGCTTCTTCAACTGGACCTGCTCCTCCAGTGTCAAAGAAGTTTGTCAAGAGTGCAAGCTTCAGTCCTAAGCAAAACAACTCTAGCACATCAGCTACCGGAGCTGCAGAGAGATTGCCAAACCCAAAGTCAGCACCATCTACCCCAGCTTCTGTTGATCAAGTCATCAAGTCTGTAAGTCCGATCAGTGTTCAGTCAACTGGAAAACTCTTTTCCTACAAAGAAGTTGCATTGGCTCCACCTGGGACAATTGTGAAGGCAGTGGCGGAGCAGTTACCAAAGCCGATTGTACAAATTTCTCAGGTGGGCCAGGAGACTCCTACAACAGAGGTCACTGTTGGTGAAGTGACGGCAATTAAAGATGTGAAGGATAAAAAAAAAAAGAAATCGACAGGAGAGAAACAGGTTATGGTTTCTGAGAAAAGCCCTTCTGTAGTACGATCAGCAAAACTGAAGGATTCTGTGGCAAAGGAGTCCTTGGAAGTTGTGAAGCATGCTAGTGTTGGAGTACAAGTTGAAGCTGAATCTGAAGCTGTGGAATTAGAAAACACTGCTTTTGAAGGTGCAGAAGTTGAAAATGTAAAAGTTCCTGTTCCTAGCGTTGAGATTTCTGATGCTTCCCAAAGTCCAAATACCACAGCTGCTGAATGTGGACCATCAGACCCCCTAGGGCCAGACAGTTGCCCCGGGACTTCCTCTGATTCAGAACCTGCATCTGCTTTAACTGAGACTAGAACCAACAATCCTTCTAATACAGGGGAGGGAAAGTCACGTGATTTACCCAATGGCGACATAGTTAAACCAGTGCCAACTGGTGGGGAAAAGGTAGATGAACAAGAAACTGGAAAGGAGACGAGCAAAAAACTTTCTGCAGCGGCACCTCCTTTTAATCCATCCCCCATTCCAGTTTTTGGCTCAATTCCGGTTCCAGTTCCAGGTCTCAAAGATCATGGAGGTATTTTGCCCCCACCTGTAAATATTCCTCCGATGCTTGCAGTTAATCCTGTCCGTAGATCACCCCATCAGTCAGCGACAGCAAGAGTTCCTTATGGTCCACGACTTTCTGGTGGCTATAACAGATCTGGAAGTCGGGTTTCACGTAATAAACATAGCTTTCACAATGGTGAGCACACCGGGGATGGTCCTCCCAGAATAATGAACCCACATGCAGCTGAGTTTGTACCTGGCCAACCTTGGGTTCAAAATGGCTATCCACTGTCTCCCAATGGTTTCTTGGCTTCCCCAAATGGTTATCCAGTGTCCCCAAATGGATATCCGGTATCACCGAATGGTACGCCGGTGAATCAAAACGGGTCTCCGACATCTCCAGTCAGTTCAGATGAGTCATCACCAGTTGTGACTGCTGATATTGGTGTTGGAGCAAATACTGAAGGTGCAGGGAAGGAGACTAATGATAAGTTATCAGTACAAGTTGAATGTGACAAGCAACCAATTGAAGGAGAGCTGCAGGAAGAACAGTCTTTAGATAATGTAAACGTTCCTCCTGAAACTGAAGAAAAGCCTACTGATACCGATACAGTTCCTGGTGATACTATTGTGGAAAAGGAGACTAGCAATCTAGTAGTTGAGGAAAAACCAAGCAAATGTTGGGGAGATTATAGCGATAATGAAGCTGAGGTCATTGAGGTTAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1848

Amino Acids

200.96

Weight (kDa)

6.07

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 48 - 118 3.1e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 717 - 860 6.5e-23 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 901 - 962 2e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 901 - 1000 2.8e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 930 - 1000 1.4e-12 Tetratricopeptide repeat
TPR_10 PF13374 930 - 971 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 1014 - 1088 2.1e-10 Tetratricopeptide repeat
TPR_10 PF13374 1014 - 1054 4.2e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 368
Acc16I TGCGCA 1 cut(s) 3290
Acc36I ACCTGC 3 cut(s) 3898, 3916, 4606
Acc65I GGTACC 1 cut(s) 3108
AccB1I GGYRCC 2 cut(s) 3108, 4765
AccB7I CCANNNNNTGG 4 cut(s) 2162, 2278, 4703, 4799
AccI GTMKAC 2 cut(s) 1116, 3652
AccII CGCG 2 cut(s) 2026, 2223
AciI CCGC 7 cut(s) 531, 554, 935, 2026, 3302, 4154, 4763
AclI AACGTT 1 cut(s) 5385
AclWI GGATC 2 cut(s) 158, 3291
AcoI YGGCCR 3 cut(s) 565, 800, 5074
AcsI RAATTY 7 cut(s) 854, 1618, 1933, 3255, 3550, 3580, 4182
AcuI CTGAAG 9 cut(s) 1527, 1556, 1743, 3627, 3934, 4361, 4437, 5298, 5421
AcvI CACGTG 1 cut(s) 4661
AcyI GRCGYC 1 cut(s) 365
AdeI CACNNNGTG 2 cut(s) 226, 1397
AflII CTTAAG 1 cut(s) 2057
AflIII ACRYGT 3 cut(s) 191, 2221, 3017
AhdI GACNNNNNGTC 1 cut(s) 2590
AjnI CCWGG 7 cut(s) 2214, 3502, 4129, 4197, 4822, 5071, 5431
AjuI GAANNNNNNNTTGG 2 cut(s) 5070, 5102
AleI CACNNNNGTG 1 cut(s) 5010
AloI GAACNNNNNNTCC 2 cut(s) 4615, 4647
Alw21I GWGCWC 4 cut(s) 474, 849, 2998, 5019
Alw44I GTGCAC 1 cut(s) 2994
AlwI GGATC 2 cut(s) 158, 3291
AlwNI CAGNNNCTG 7 cut(s) 2045, 3446, 3527, 4040, 4532, 4598, 5432
Ama87I CYCGRG 3 cut(s) 3272, 3314, 4574
ApaLI GTGCAC 1 cut(s) 2994
ApoI RAATTY 7 cut(s) 854, 1618, 1933, 3255, 3550, 3580, 4182
ArsI GACNNNNNNTTYG 6 cut(s) 481, 513, 3260, 3292, 4001, 4033
AseI ATTAAT 1 cut(s) 2880
Asp700I GAANNNNTTC 1 cut(s) 836
Asp718I GGTACC 1 cut(s) 3108
AspA2I CCTAGG 2 cut(s) 3626, 4555
AspLEI GCGC 1 cut(s) 3291
AsuC2I CCSGG 4 cut(s) 402, 4575, 4576, 5023
AsuII TTCGAA 1 cut(s) 334
AsuNHI GCTAGC 1 cut(s) 3173
AvaI CYCGRG 3 cut(s) 3272, 3314, 4574
AvrII CCTAGG 2 cut(s) 3626, 4555
AxyI CCTNAGG 1 cut(s) 1479
BaeGI GKGCMC 1 cut(s) 2998
BaeI ACNNNNGTAYC 4 cut(s) 5172, 5205, 5430, 5463
BalI TGGCCA 1 cut(s) 5076
BanI GGYRCC 2 cut(s) 3108, 4765
BarI GAAGNNNNNNTAC 2 cut(s) 1588, 1620
BauI CACGAG 1 cut(s) 221
BbrPI CACGTG 1 cut(s) 4661
BbsI GAAGAC 4 cut(s) 343, 375, 494, 2798
Bbv12I GWGCWC 4 cut(s) 474, 849, 2998, 5019
BbvCI CCTCAGC 2 cut(s) 1449, 5523
BceAI ACGGC 7 cut(s) 305, 464, 618, 787, 3010, 3057, 4254
BcgI CGANNNNNNTGC 4 cut(s) 458, 492, 2004, 2038
BciT130I CCWGG 7 cut(s) 2216, 3504, 4131, 4199, 4824, 5073, 5433
BciVI GTATCC 3 cut(s) 558, 2279, 2422
BclI TGATCA 3 cut(s) 2902, 3154, 4045
BcnI CCSGG 4 cut(s) 402, 4575, 4576, 5023
BfmI CTRYAG 6 cut(s) 711, 2814, 3993, 4320, 4758, 5354
BfrI CTTAAG 1 cut(s) 2057
BfuAI ACCTGC 3 cut(s) 3898, 3916, 4606
BfuI GTATCC 3 cut(s) 558, 2279, 2422
BglII AGATCT 3 cut(s) 626, 3418, 4967
BlnI CCTAGG 2 cut(s) 3626, 4555
BmcAI AGTACT 2 cut(s) 1653, 2820
BmeRI GACNNNNNGTC 1 cut(s) 2590
BmeT110I CYCGRG 3 cut(s) 3272, 3314, 4574
BmrI ACTGGG 3 cut(s) 2841, 3780, 3820
BmtI GCTAGC 1 cut(s) 3177
BmuI ACTGGG 3 cut(s) 2841, 3780, 3820
BpiI GAAGAC 4 cut(s) 343, 375, 494, 2798
BplI GAGNNNNNCTC 4 cut(s) 3203, 3235, 3509, 3541
BpmI CTGGAG 6 cut(s) 888, 2735, 2814, 3376, 3900, 5199
Bpu10I CCTNAGC 3 cut(s) 1449, 3957, 5523
Bpu14I TTCGAA 1 cut(s) 334
BpuEI CTTGAG 5 cut(s) 113, 665, 2576, 3110, 3677
BpuMI CCSGG 4 cut(s) 402, 4575, 4576, 5023
BsaAI YACGTR 3 cut(s) 184, 4661, 4988
BsaBI GATNNNNATC 2 cut(s) 512, 2841
BsaHI GRCGYC 1 cut(s) 365
BsaI GGTCTC 3 cut(s) 2054, 4832, 5208
BsaWI WCCGGW 5 cut(s) 424, 449, 3986, 4810, 5164
Bse118I RCCGGY 5 cut(s) 518, 798, 1804, 3744, 5185
Bse21I CCTNAGG 1 cut(s) 1479
Bse3DI GCAATG 4 cut(s) 549, 1050, 2489, 3072
Bse8I GATNNNNATC 2 cut(s) 512, 2841
BseBI CCWGG 7 cut(s) 2216, 3504, 4131, 4199, 4824, 5073, 5433
BseJI GATNNNNATC 2 cut(s) 512, 2841
BseMI GCAATG 4 cut(s) 549, 1050, 2489, 3072
BseRI GAGGAG 5 cut(s) 594, 674, 1079, 1970, 3903
BseSI GKGCMC 1 cut(s) 2998
BseX3I CGGCCG 1 cut(s) 800
BseYI CCCAGC 1 cut(s) 4033
BsgI GTGCAG 5 cut(s) 343, 2094, 3339, 4473, 5304
Bsh1236I CGCG 2 cut(s) 2026, 2223
Bsh1285I CGRYCG 3 cut(s) 434, 516, 803
BshNI GGYRCC 2 cut(s) 3108, 4765
BsiEI CGRYCG 3 cut(s) 434, 516, 803
BsiHKAI GWGCWC 4 cut(s) 474, 849, 2998, 5019
BsiHKCI CYCGRG 3 cut(s) 3272, 3314, 4574
BslFI GGGAC 7 cut(s) 884, 2232, 2585, 2612, 4147, 4591, 5134
BsmBI CGTCTC 4 cut(s) 984, 2598, 3033, 4733
BsmFI GGGAC 7 cut(s) 884, 2232, 2585, 2612, 4147, 4591, 5134
BsmI GAATGC 2 cut(s) 1314, 1551
Bso31I GGTCTC 3 cut(s) 2054, 4832, 5208
BsoBI CYCGRG 3 cut(s) 3272, 3314, 4574
Bsp119I TTCGAA 1 cut(s) 334
Bsp1286I GDGCHC 4 cut(s) 474, 849, 2998, 5019
Bsp1407I TGTACA 3 cut(s) 2254, 2634, 4177
Bsp19I CCATGG 1 cut(s) 1013
BspACI CCGC 7 cut(s) 531, 554, 935, 2026, 3302, 4154, 4763
BspFNI CGCG 2 cut(s) 2026, 2223
BspHI TCATGA 1 cut(s) 1378
BspMAI CTGCAG 3 cut(s) 3997, 4762, 5358
BspMI ACCTGC 3 cut(s) 3898, 3916, 4606
BspOI GCTAGC 1 cut(s) 3177
BspPI GGATC 2 cut(s) 158, 3291
BspQI GCTCTTC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 334
BspT107I GGYRCC 2 cut(s) 3108, 4765
BspTI CTTAAG 1 cut(s) 2057
BspTNI GGTCTC 3 cut(s) 2054, 4832, 5208
BsrDI GCAATG 4 cut(s) 549, 1050, 2489, 3072
BsrFI RCCGGY 5 cut(s) 518, 798, 1804, 3744, 5185
BsrGI TGTACA 3 cut(s) 2254, 2634, 4177
BssAI RCCGGY 5 cut(s) 518, 798, 1804, 3744, 5185
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 8 cut(s) 39, 1013, 2567, 2697, 3626, 4365, 4555, 5081
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 7 cut(s) 2216, 3504, 4131, 4199, 4824, 5073, 5433
Bst6I CTCTTC 5 cut(s) 57, 291, 306, 982, 2462
BstACI GRCGYC 1 cut(s) 365
BstAFI CTTAAG 1 cut(s) 2057
BstAPI GCANNNNNTGC 3 cut(s) 1259, 1744, 3259
BstAUI TGTACA 3 cut(s) 2254, 2634, 4177
BstBAI YACGTR 3 cut(s) 184, 4661, 4988
BstBI TTCGAA 1 cut(s) 334
BstDSI CCRYGG 2 cut(s) 321, 1013
BstENI CCTNNNNNAGG 2 cut(s) 1509, 1642
BstFNI CGCG 2 cut(s) 2026, 2223
BstHHI GCGC 1 cut(s) 3291
BstMCI CGRYCG 3 cut(s) 434, 516, 803
BstNI CCWGG 7 cut(s) 2216, 3504, 4131, 4199, 4824, 5073, 5433
BstNSI RCATGY 8 cut(s) 195, 1731, 2367, 2645, 2652, 3021, 4385, 5057
BstSFI CTRYAG 6 cut(s) 711, 2814, 3993, 4320, 4758, 5354
BstSLI GKGCMC 1 cut(s) 2998
BstUI CGCG 2 cut(s) 2026, 2223
BstV2I GAAGAC 4 cut(s) 343, 375, 494, 2798
BstX2I RGATCY 5 cut(s) 150, 626, 3283, 3418, 4967
BstXI CCANNNNNNTGG 2 cut(s) 2549, 3337
BstYI RGATCY 5 cut(s) 150, 626, 3283, 3418, 4967
BstZI CGGCCG 1 cut(s) 800
Bsu36I CCTNAGG 1 cut(s) 1479
BsuI GTATCC 3 cut(s) 558, 2279, 2422
BtgI CCRYGG 2 cut(s) 321, 1013
BtsI GCAGTG 2 cut(s) 4155, 4437
BveI ACCTGC 3 cut(s) 3898, 3916, 4606
CaiI CAGNNNCTG 7 cut(s) 2045, 3446, 3527, 4040, 4532, 4598, 5432
CciI TCATGA 1 cut(s) 1378
CfoI GCGC 1 cut(s) 3291
Cfr10I RCCGGY 5 cut(s) 518, 798, 1804, 3744, 5185
Cfr9I CCCGGG 1 cut(s) 4574
CseI GACGC 1 cut(s) 2229
CsiI ACCWGGT 1 cut(s) 3502
CspCI CAANNNNNGTGG 2 cut(s) 2106, 2141
DraIII CACNNNGTG 2 cut(s) 226, 1397
DriI GACNNNNNGTC 1 cut(s) 2590
EaeI YGGCCR 3 cut(s) 565, 800, 5074
EagI CGGCCG 1 cut(s) 800
Eam1104I CTCTTC 5 cut(s) 57, 291, 306, 982, 2462
Eam1105I GACNNNNNGTC 1 cut(s) 2590
EarI CTCTTC 5 cut(s) 57, 291, 306, 982, 2462
EciI GGCGGA 2 cut(s) 543, 4169
EclXI CGGCCG 1 cut(s) 800
Eco130I CCWWGG 8 cut(s) 39, 1013, 2567, 2697, 3626, 4365, 4555, 5081
Eco147I AGGCCT 1 cut(s) 1091
Eco31I GGTCTC 3 cut(s) 2054, 4832, 5208
Eco32I GATATC 3 cut(s) 1867, 3839, 5162
Eco52I CGGCCG 1 cut(s) 800
Eco57I CTGAAG 9 cut(s) 1527, 1556, 1743, 3627, 3934, 4361, 4437, 5298, 5421
Eco72I CACGTG 1 cut(s) 4661
Eco81I CCTNAGG 1 cut(s) 1479
Eco88I CYCGRG 3 cut(s) 3272, 3314, 4574
EcoNI CCTNNNNNAGG 2 cut(s) 1509, 1642
EcoO109I RGGNCCY 4 cut(s) 233, 664, 1635, 1816
EcoRI GAATTC 1 cut(s) 1933
EcoRII CCWGG 7 cut(s) 2214, 3502, 4129, 4197, 4822, 5071, 5431
EcoRV GATATC 3 cut(s) 1867, 3839, 5162
EcoT14I CCWWGG 8 cut(s) 39, 1013, 2567, 2697, 3626, 4365, 4555, 5081
EcoT22I ATGCAT 2 cut(s) 2017, 2190
ErhI CCWWGG 8 cut(s) 39, 1013, 2567, 2697, 3626, 4365, 4555, 5081
Esp3I CGTCTC 4 cut(s) 984, 2598, 3033, 4733
FalI AAGNNNNNCTT 6 cut(s) 2681, 2713, 4349, 4381, 4918, 4950
FaqI GGGAC 7 cut(s) 884, 2232, 2585, 2612, 4147, 4591, 5134
FauI CCCGC 1 cut(s) 942
FbaI TGATCA 3 cut(s) 2902, 3154, 4045
FblI GTMKAC 2 cut(s) 1116, 3652
FspI TGCGCA 1 cut(s) 3290
GlaI GCGC 1 cut(s) 3290
GsaI CCCAGC 1 cut(s) 4037
GsuI CTGGAG 6 cut(s) 888, 2735, 2814, 3376, 3900, 5199
HgaI GACGC 1 cut(s) 2229
HhaI GCGC 1 cut(s) 3291
Hin1I GRCGYC 1 cut(s) 365
Hin6I GCGC 1 cut(s) 3289
HinP1I GCGC 1 cut(s) 3289
HincII GTYRAC 4 cut(s) 346, 1117, 1534, 4086
HindII GTYRAC 4 cut(s) 346, 1117, 1534, 4086
HindIII AAGCTT 2 cut(s) 3333, 3946
HpaI GTTAAC 1 cut(s) 1534
Hpy99I CGWCG 7 cut(s) 296, 356, 367, 370, 382, 470, 709
Hsp92I GRCGYC 1 cut(s) 365
HspAI GCGC 1 cut(s) 3289
KpnI GGTACC 1 cut(s) 3112
KroI GCCGGC 2 cut(s) 518, 798
KroNI GCCGGC 2 cut(s) 520, 800
Ksp22I TGATCA 3 cut(s) 2902, 3154, 4045
KspAI GTTAAC 1 cut(s) 1534
LguI GCTCTTC 1 cut(s) 306
MabI ACCWGGT 1 cut(s) 3502
MfeI CAATTG 2 cut(s) 4137, 5340
MflI RGATCY 5 cut(s) 150, 626, 3283, 3418, 4967
MhlI GDGCHC 4 cut(s) 474, 849, 2998, 5019
MlsI TGGCCA 1 cut(s) 5076
MluI ACGCGT 1 cut(s) 2221
MluNI TGGCCA 1 cut(s) 5076
MlyI GAGTC 8 cut(s) 122, 455, 511, 545, 3335, 4198, 4370, 5240
MmeI TCCRAC 3 cut(s) 4372, 5231, 5245
Mox20I TGGCCA 1 cut(s) 5076
Mph1103I ATGCAT 2 cut(s) 2017, 2190
MreI CGCCGGCG 1 cut(s) 518
MroNI GCCGGC 2 cut(s) 518, 798
MroXI GAANNNNTTC 1 cut(s) 836
MscI TGGCCA 1 cut(s) 5076
MslI CAYNNNNRTG 3 cut(s) 3408, 4386, 5010
Msp20I TGGCCA 1 cut(s) 5076
MspA1I CMGCKG 4 cut(s) 1358, 4529, 4763, 5060
MspCI CTTAAG 1 cut(s) 2057
MunI CAATTG 2 cut(s) 4137, 5340
Mva1269I GAATGC 2 cut(s) 1314, 1551
MvaI CCWGG 7 cut(s) 2216, 3504, 4131, 4199, 4824, 5073, 5433
MvnI CGCG 2 cut(s) 2026, 2223
NaeI GCCGGC 2 cut(s) 520, 800
NciI CCSGG 4 cut(s) 402, 4575, 4576, 5023
NcoI CCATGG 1 cut(s) 1013
NgoMIV GCCGGC 2 cut(s) 518, 798
NheI GCTAGC 1 cut(s) 3173
NmeAIII GCCGAG 2 cut(s) 543, 582
NmuCI GTSAC 9 cut(s) 121, 1939, 2061, 3747, 4219, 4234, 4656, 5245, 5327
NsbI TGCGCA 1 cut(s) 3290
NsiI ATGCAT 2 cut(s) 2017, 2190
NspI RCATGY 8 cut(s) 195, 1731, 2367, 2645, 2652, 3021, 4385, 5057
NspV TTCGAA 1 cut(s) 334
OliI CACNNNNGTG 1 cut(s) 5010
PaeI GCATGC 1 cut(s) 4385
PaeR7I CTCGAG 1 cut(s) 3314
PagI TCATGA 1 cut(s) 1378
PasI CCCWGGG 1 cut(s) 2215
PceI AGGCCT 1 cut(s) 1091
PciI ACATGT 2 cut(s) 191, 3017
PciSI GCTCTTC 1 cut(s) 306
PcsI WCGNNNNNNNCGW 4 cut(s) 374, 465, 999, 4984
PctI GAATGC 2 cut(s) 1314, 1551
PdiI GCCGGC 2 cut(s) 520, 800
PdmI GAANNNNTTC 1 cut(s) 836
PfeI GAWTC 9 cut(s) 508, 1187, 2036, 2681, 3644, 4346, 4412, 4589, 5191
PflFI GACNNNGTC 1 cut(s) 350
PflMI CCANNNNNTGG 4 cut(s) 2162, 2278, 4703, 4799
Ple19I CGATCG 1 cut(s) 516
PleI GAGTC 8 cut(s) 121, 454, 510, 544, 3334, 4198, 4369, 5239
PmaCI CACGTG 1 cut(s) 4661
PmlI CACGTG 1 cut(s) 4661
PpsI GAGTC 8 cut(s) 121, 454, 510, 544, 3334, 4198, 4369, 5239
Ppu21I YACGTR 3 cut(s) 184, 4661, 4988
PpuMI RGGWCCY 3 cut(s) 233, 664, 1635
PscI ACATGT 2 cut(s) 191, 3017
PshBI ATTAAT 1 cut(s) 2880
Psp1406I AACGTT 1 cut(s) 5385
Psp5II RGGWCCY 3 cut(s) 233, 664, 1635
Psp6I CCWGG 7 cut(s) 2214, 3502, 4129, 4197, 4822, 5071, 5431
PspCI CACGTG 1 cut(s) 4661
PspFI CCCAGC 1 cut(s) 4033
PspGI CCWGG 7 cut(s) 2214, 3502, 4129, 4197, 4822, 5071, 5431
PspPPI RGGWCCY 3 cut(s) 233, 664, 1635
PspXI VCTCGAGB 1 cut(s) 3314
PstI CTGCAG 3 cut(s) 3997, 4762, 5358
PstNI CAGNNNCTG 7 cut(s) 2045, 3446, 3527, 4040, 4532, 4598, 5432
PsuI RGATCY 5 cut(s) 150, 626, 3283, 3418, 4967
PsyI GACNNNGTC 1 cut(s) 350
PvuI CGATCG 1 cut(s) 516
PvuII CAGCTG 3 cut(s) 1358, 4529, 5060
RseI CAYNNNNRTG 3 cut(s) 3408, 4386, 5010
SalI GTCGAC 1 cut(s) 1115
SapI GCTCTTC 1 cut(s) 306
ScaI AGTACT 2 cut(s) 1653, 2820
SchI GAGTC 8 cut(s) 122, 455, 511, 545, 3335, 4198, 4370, 5240
SduI GDGCHC 4 cut(s) 474, 849, 2998, 5019
SexAI ACCWGGT 1 cut(s) 3502
SfcI CTRYAG 6 cut(s) 711, 2814, 3993, 4320, 4758, 5354
Sfr274I CTCGAG 1 cut(s) 3314
SfuI TTCGAA 1 cut(s) 334
SgrAI CRCCGGYG 2 cut(s) 518, 5185
SlaI CTCGAG 1 cut(s) 3314
SmaI CCCGGG 1 cut(s) 4576
SmiMI CAYNNNNRTG 3 cut(s) 3408, 4386, 5010
SmlI CTYRAG 7 cut(s) 128, 644, 2057, 2555, 3125, 3314, 3656
SmoI CTYRAG 7 cut(s) 128, 644, 2057, 2555, 3125, 3314, 3656
SphI GCATGC 1 cut(s) 4385
SseBI AGGCCT 1 cut(s) 1091
SsiI CCGC 7 cut(s) 531, 554, 935, 2026, 3302, 4154, 4763
SspI AATATT 3 cut(s) 2374, 3672, 4867
StuI AGGCCT 1 cut(s) 1091
StyI CCWWGG 8 cut(s) 39, 1013, 2567, 2697, 3626, 4365, 4555, 5081
TaqII GACCGA 1 cut(s) 420
TatI WGTACW 8 cut(s) 1604, 1651, 2254, 2634, 2818, 4177, 4396, 5314
TauI GCSGC 4 cut(s) 534, 2029, 3305, 4766
TfiI GAWTC 9 cut(s) 508, 1187, 2036, 2681, 3644, 4346, 4412, 4589, 5191
TseFI GTSAC 9 cut(s) 121, 1939, 2061, 3747, 4219, 4234, 4656, 5245, 5327
Tsp45I GTSAC 9 cut(s) 121, 1939, 2061, 3747, 4219, 4234, 4656, 5245, 5327
TspGWI ACGGA 5 cut(s) 158, 323, 627, 3373, 4886
TspMI CCCGGG 1 cut(s) 4574
Tth111I GACNNNGTC 1 cut(s) 350
Van91I CCANNNNNTGG 4 cut(s) 2162, 2278, 4703, 4799
Vha464I CTTAAG 1 cut(s) 2057
VneI GTGCAC 1 cut(s) 2994
VspI ATTAAT 1 cut(s) 2880
XagI CCTNNNNNAGG 2 cut(s) 1509, 1642
XapI RAATTY 7 cut(s) 854, 1618, 1933, 3255, 3550, 3580, 4182
XbaI TCTAGA 1 cut(s) 946
XceI RCATGY 8 cut(s) 195, 1731, 2367, 2645, 2652, 3021, 4385, 5057
XcmI CCANNNNNNNNNTGG 3 cut(s) 2695, 5119, 5493
XhoI CTCGAG 1 cut(s) 3314
XmaI CCCGGG 1 cut(s) 4574
XmaJI CCTAGG 2 cut(s) 3626, 4555
XmiI GTMKAC 2 cut(s) 1116, 3652
XmnI GAANNNNTTC 1 cut(s) 836
ZraI GACGTC 1 cut(s) 366
ZrmI AGTACT 2 cut(s) 1653, 2820
Zsp2I ATGCAT 2 cut(s) 2017, 2190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.