Rw4G010680

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011973
Physical Location & Seq
Reverse (-)
24865346 .. 24880816
15471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010680.1

Sequence Viewer

Length: 5469 bp
ATGGCTCCCAAAACAGGGAAAGCCAAGCCACACAAATCCAAGGGAGACAAGAAGAAGAAAGAAGAGAAAGATATTGTTTATATTATCTCCTTATCTTTTGAAAGAAAACAAGAAAGCAAAAAGATCTTAGATGTGAGAAAGCTGTTGGGGGTCCACGTAGATACATGTCATTCACCAATTTCTCCCTATCACACGAGTCGTAATCAGGTGCGAGGACCCAGACTGAAAGATACTGTGGACATCCTCTCTCTCAAACCCTGCCACCTCACCATCGTCGAAGAGGACTACACGGAAGAGCAAGCCGTGGCTCACATTCGAAGACTGGTTGACATCGTCGCCTGCACGACGTCGTTTGGCTCGTCGTCTACTTCACCCAGAACCCCCGGTTCTGCTTCGGTTCCGGCTCCGGTTCGGTCGAGTTCTAAAGAGTCCGGTTTAGACGAGGGCGACGAGCACAATGCGGTTCCGAAGACCAAAGTTTCGAGTCCGATTCCGATCGCCGGCGAGAAAGGCGGCGAGTCGGCAATGTATCCTCCGCCGAGGCTGGGCCAGTTTTACGACTTCTTCTCCTTGTCTCACCTTACTCCTCCACTCCACTACGTTAGAAGATCTAGCCGTCCGTTCCTTGAGGACAAGGCAGAGGAGGACCTGTTTCAAATTGATGCATTTGGGGTTAAATTGGTGATTGCAGGTTCGGTTTGTAGTGGGAAGCCGACGACTATAGTAGCATCCAGAAAAGGGTTCTACCCTGCTGGAAAACGTCCTCTTATTACTCACTCTCTGGTTGCTTTGCTTCAACTGATAAGCCGGCCGTTCGATGCTGCATACAATGCTGTCATGAAGGCTTTCACCGAGCACAATAAATTTGGGAACCTTCCTTATGGTTTTCGGGCAAACACATGGGTTGTCCCTCCAGTTGTTGCTGAGAACCCGTCTGTTTTCCCGCCACTTCCTCTAGAAGATGAAAATTGGGGAGGAAATGGGGGTGGGCAGGGAAGAGACGGTAAACACGATAACAGGCCATGGGCGAAAGAGTTTGCCATTCTTGCAGCAATGCCTTGTGCAACAGCAGAGGAGAGGCAAGTTCGAGATCGAAAGGCCTTTCTGCTTCACAGTTTATTTGTCGACGTCTCAGTTCTTAAAGCTGTTGCAGCTATTAAGAGCCTAATTGACACTCATCAATCTTCCCTAAATGATTCCACTCTTTCACTTCATCATGAGGAAAAAATTGGAGATTTAATCATTAAGATCGTTAGAGATGCACCTGATGCAAGTGTAAAGGTGGATTGCAAGAATGATGGTAGTCGAGTTCTAGGAATGCCACAAGAAGAAGTTACTCAGAGAAACCTACTCAAAGGCATAACAGCTGATGAGAGTGCAACTGTTCATGATACTTCCACACTAGGTGTTGTGGTTGTTAGGCATTGTGGATTTACTGCTGTTGTGAAAGTTTTAGCTGAGGTGAATTGGGTGGGGAAACCTGTTCCTCAGGACATTGAAATTGAGGACCAGCCTGAAGGAGGTGCAAATGCCTTGAATGTTAACAGCCTGAGAATGCTATTGCAACAGTCATCCCTCCTTCAGTCATCAACTACGGTTCAACGAAGCCAGAGCACAGATTTTGAAAATTTACGTTCTGCTAGGTCCTTAGTAAGGAAAGTACTCGAAGAAAGTTTATTAAGGTTGCAGGGTGGGCCATCTAATCACACCAAGTCGATCAGATGGGAGCTAGGAGCATGTTGGGTGCAACATTTGCAAAATCAGGCTTCAGCGAAAAATGAGCCTAAGAAAAATGAAGAAGCTAAGATAGAACCGGCTGTTAAGGGCCTCGGAAAGCAAGGTGGACTGCTAAAGGAAATAAAGAAGAAAATGGATATCAGGAGCAGTAAAACTGAACAGGGCAAGGAAGTTCTTGGGGGTAACAACCTTGATCTTAATAAGAATTCAGTCACCACCAGTCAGGAGGAGTTAGAAAAGCGAGATGCCGAAAAGGAAATAAATTGGAGAAGGCTACTTCCTGATGCATCCTATTCGCGGCTCAAAGAATCAGATACTGGTCTCCACCTTAAGTCACCTGATGAGTTGATTGAAATGGCACACAAGTATTACGCTGATACTGCACTTCCAAAACTGGTGGCAGATTTTGGCTCCCTTGAACTTTCACCAGTTGATGGAAGGACATTGACAGATTTTATGCATACAAGGGGTTTGAAAATGTGTTCCCTGGGACGCGTGGTTGAACTTGCAGATAAGCTCCCTCATGTACAATCACTCTGTATCCATGAGATGGTTGTGCGAGCATACAAACACATACTGCAAGCTGTTGTAGCAGCAGTTGATAATGTCGCAGACTTGGCTGCATCCATAGCAGCATGTCTAAATATTCTGCTAGGAACCCCATCACCTGAAAATGTGGATGGAGACATTACTTGTGAGGATACGTTAAAATGGAAGTGGGTGGAAACATTCCTTTTGAAGAGGTTTGGATGGCAATGGAAACATGAAAGCGTTGAGGACTTTAGAAAGTTTGCCATTCTTCGTGGATTGTGCCATAAGGTTGGCCTTGAGCTTGTGCCAAGGGATTATGATATGGACACTTCATCTCCTTTTAGGAAATCAGATATAGTAAGCATGGTCCCTGTGTACAAGCATGTTGCATGTTCATCTGCTGATGGACGTACACTTTTGGAATCTTCCAAGACTTCCTTGGATAAAGGTAAACTGGAGGATGCTGTTAATTATGGCACTAAGGCGTTGTCAAAACTTGTATCAGTATGTGGGCCTTATCATCGAATGACTGCTGGAGCATATAGTCTTCTCGCTGTAGTACTGTATCACACTGGGGATTTTAATCAGGCAACCATCTATCAGCAAAAAGCATTGGATATTAATGAGAGAGAGCTTGGACTTGATCATCCTGATACAATGAAAAGTTACGGGGATTTAGCTGTGTTCTACTACCGACTTCAACATACAGAGTTGGCCTTGAAGTACGTCAATCGTGCGCTGTATCTTTTGCACCTAACATGTGGGCCGTCTCATCCAAACACGGCTGCCACCTATATCAATGTAGCAATGATGGAAGAAGGTTTGGGGAACGTCCATGTTGCTCTTAGGTACCTCCATGAAGCTCTCAAGTGCAATCAAAGGCTACTTGGAGCTGATCATATACAGACTGCTGCTAGCTATCATGCCATAGCTATCGCACTTTCTTTGATGGAGGCATACTCATTAAGTGTTCAGCACGAACAAACTACCTTGCAAATTCTGCAGGCGAAACTCGGGTCAGAGGATCTACGCACACAGGATGCGGCTGCATGGCTTGAGTATTTTGAGTCCAAAGCTTTGGAGCAGCAAGAAGCTGCACGGAATGGTACTCCAAAGCCAGATGCTTCAATCTCCAGCAAAGGTCATTTAAGTGTATCAGATCTTTTGGATTATATAACACCAGATGCTGATATGAAAGCTAGAGAGGCCCAAAGGAAAGCTCGTTTGAAGGTTAAAGGCAAACCAGGTCAGAACGGGGAGGCAGTCTCTGATGAGTACCAGAAGGATGAAAATTTGTTACCAAGTCATCCTGTTGCAGAGAATTTAAGTGATAAAGAGAACAAGTCTGAAGCACAATTAGGAGAACCTAGGAATGAGAAATATGATTCCCGTCTACTTGAGCAATCAATAATATATGCTACACATGATGATCTGGCACAAGATGACAACTCAGATGAAGGCTGGCAAGAGGCTGTTCCTAAAGGCCGGTCACTTATAGGTCGCAAATCTACTGGGTCAAGGAGGCCAAGCCTTGAAAAACTGAATACTAACTTTATCAATACATCCCAGTCATCAAGATATCGAGGCAAGGCCAATAATTTCACATCCCCCAAAACAAGCCCAAATGAGCCTGCTTCTTCAACTGGACCTGCTCCTCCAGTGTCAAAGAAGTTTGTCAAGAGTGCAAGCTTCAGCCCTAAGCAAAACAACTCTAGCACATCAGCTACCGGAGCTGCAGAGAGATTGCCAAACCCAAAGTCAGCACCATCTACCCCAGCTTCTGTTGATCAAGTCATCAAGTCTGTAAGTCCGATCAGTGTTCAGTCAACTGGAAAACTCTTTTCCTACAAAGAAGTTGCAGTGGCTCCACCTGGGACAATTGTGAAGGCAGTGGCGGAGCAGTTACCAAAGCCGATTGTACAAATTTCTCAGGTGGGCCAGGAGACACCTACAACAGAGGTCACTGTTGGTGAAGTGACGGCAATTAAAGATGTGAAGGATAAAAAAAATAAGAAATCGACAGGAGAGAAACAGGTTATGGTTTCTGAGAAAATCCCTTCTGTAGTACGATCAGCAAAACTGAAGGATTCTGTGGCAAAGGTGTCCTTGGAAGTTGTGAAGCATGCTAGTGTTGGAGTACAAGTTGAAGCTGAATCTGAAGCTGTGGAATTAGAAAACACTGCTTTTAAAGGTGCAGAAGTTGAAAATGTAAAAGTTCCTGTTCCTAGCGTTGAGATTTCTGATGCTTCCCAAAGTCCAAATACCACAGCTGCTGAATGTGGACCATCAGACCCCCTAGGGCCAGACAGTTGCCCCAGGACTTCCTCTGATTCAGAACCTCCATCTGGAAAGTCACGTGATTTACCCAATGGCGACATAGTTAAACCAGTGCCAACTGGTGGGGAAAAGGTAGATGAACAAGAAACTGGAAAGGAGACGAGCAAAAAACTTTCTGCAGCCGCACCTCCTTTTAATCCATCCCCCATTCCAGTTTTTGGCTCAATTCCGGTTCCAGTTCCAGGTCTCAAAGAGCATGGAGGGATTTTGCCCCCACCTGTAAATATTCCTCCGATGCTTGCAGTTAATCCTGTCCGTAGATCACCCCATCAGTCAGCGACAGCAAGAGTTCCTTATGGTCCACGACTTTCTGGTGGCTATAACAGATCTGGAAGTCGGGTTTCACGTAATAAACATAGCTTTCACAATGGTGAGCACACCGGGGATGGTCCTCCCAGAATAATGAACCCACATGCAGCTGAGTTTGTACCTGGCCAACCTTGGGTTCAAAATGGCTATCCACTGTCTCCCAATGGTTTCTTGCCTTCCCCAAATGGTTATCCAGTGTCCCCAAATGGATATCCGGTATCACCAAATGGTACTCCGGTGAATCAAAACGGGTCTCCGACATCTCCAGTCAGTTCGGATGAGTCATCACCAGTTGTGATTGCTGATATTGGTGTTGGAGCAAATACTGAAGGTGCAGGGAAGGAGACTAATGATAAGTTATCAGTACAAGTTGAATTCTTTAGATATGTAAACGTTCCTCCTGAAACTGAAGAAAAGCCTATTGATACCGATACAGTTCCTGGTGATACTATTGTGGAAAAGGAGACTAGCAATCTAGTAGTTGAGGAAAAACCAAGTAAATGTTGGGGAGATTATAGCGATAATGAAGCTGAGGTCATTGAGGTTAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1822

Amino Acids

198.41

Weight (kDa)

6.57

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_p135 PF12807 720 - 863 7.3e-23 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 904 - 965 2e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 904 - 1003 2.8e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 933 - 1003 1.4e-12 Tetratricopeptide repeat
TPR_10 PF13374 933 - 974 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 1017 - 1091 2e-10 Tetratricopeptide repeat
TPR_10 PF13374 1017 - 1057 4.1e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 350, 1131
Acc36I ACCTGC 2 cut(s) 680, 3925
Acc65I GGTACC 1 cut(s) 3117
AccB1I GGYRCC 1 cut(s) 3117
AccB7I CCANNNNNTGG 4 cut(s) 2171, 2287, 4667, 4763
AccI GTMKAC 3 cut(s) 365, 1125, 3661
AccII CGCG 2 cut(s) 2035, 2232
AciI CCGC 8 cut(s) 461, 513, 536, 944, 2035, 3311, 4163, 4728
AclI AACGTT 1 cut(s) 5307
AclWI GGATC 1 cut(s) 3300
AcoI YGGCCR 2 cut(s) 809, 5038
AcsI RAATTY 8 cut(s) 863, 1627, 1942, 3264, 3559, 3589, 4191, 5288
AcuI CTGAAG 9 cut(s) 1536, 1565, 1752, 3636, 3943, 4370, 4446, 5262, 5343
AcvI CACGTG 1 cut(s) 4625
AcyI GRCGYC 2 cut(s) 347, 1128
AdeI CACNNNGTG 1 cut(s) 1406
AflII CTTAAG 1 cut(s) 2066
AflIII ACRYGT 3 cut(s) 164, 2230, 3026
AjnI CCWGG 8 cut(s) 2223, 3511, 4138, 4206, 4583, 4786, 5035, 5353
AjuI GAANNNNNNNTTGG 2 cut(s) 5034, 5066
AleI CACNNNNGTG 1 cut(s) 4974
Alw21I GWGCWC 4 cut(s) 456, 858, 1616, 4983
AlwI GGATC 1 cut(s) 3300
AlwNI CAGNNNCTG 6 cut(s) 2054, 3455, 3536, 4049, 4541, 5354
Ama87I CYCGRG 1 cut(s) 3281
ApoI RAATTY 8 cut(s) 863, 1627, 1942, 3264, 3559, 3589, 4191, 5288
ArsI GACNNNNNNTTYG 6 cut(s) 463, 495, 3269, 3301, 4010, 4042
AseI ATTAAT 1 cut(s) 2889
Asp700I GAANNNNTTC 1 cut(s) 845
Asp718I GGTACC 1 cut(s) 3117
AspA2I CCTAGG 2 cut(s) 3635, 4564
AspLEI GCGC 1 cut(s) 3007
AsuC2I CCSGG 2 cut(s) 384, 4987
AsuII TTCGAA 1 cut(s) 316
AsuNHI GCTAGC 1 cut(s) 3182
AvaI CYCGRG 1 cut(s) 3281
AvrII CCTAGG 2 cut(s) 3635, 4564
AxyI CCTNAGG 1 cut(s) 1488
BaeI ACNNNNGTAYC 4 cut(s) 5136, 5169, 5352, 5385
BalI TGGCCA 1 cut(s) 5040
BanI GGYRCC 1 cut(s) 3117
BauI CACGAG 1 cut(s) 193
BbrPI CACGTG 1 cut(s) 4625
BbsI GAAGAC 3 cut(s) 325, 476, 2807
Bbv12I GWGCWC 4 cut(s) 456, 858, 1616, 4983
BbvCI CCTCAGC 2 cut(s) 1458, 5445
BceAI ACGGC 6 cut(s) 287, 600, 796, 3019, 3066, 4263
BcgI CGANNNNNNTGC 4 cut(s) 440, 474, 2013, 2047
BciT130I CCWGG 8 cut(s) 2225, 3513, 4140, 4208, 4585, 4788, 5037, 5355
BciVI GTATCC 3 cut(s) 540, 2288, 2431
BclI TGATCA 3 cut(s) 2911, 3163, 4054
BcnI CCSGG 2 cut(s) 384, 4987
BfmI CTRYAG 6 cut(s) 720, 2823, 3269, 4002, 4329, 4722
BfrI CTTAAG 1 cut(s) 2066
BfuAI ACCTGC 2 cut(s) 680, 3925
BfuI GTATCC 3 cut(s) 540, 2288, 2431
BglII AGATCT 4 cut(s) 123, 608, 3427, 4931
BlnI CCTAGG 2 cut(s) 3635, 4564
BmcAI AGTACT 2 cut(s) 1662, 2829
BmeT110I CYCGRG 1 cut(s) 3281
BmrI ACTGGG 3 cut(s) 2850, 3789, 3829
BmtI GCTAGC 1 cut(s) 3186
BmuI ACTGGG 3 cut(s) 2850, 3789, 3829
BpiI GAAGAC 3 cut(s) 325, 476, 2807
BplI GAGNNNNNCTC 4 cut(s) 3212, 3244, 3518, 3550
BpmI CTGGAG 6 cut(s) 897, 2744, 2823, 3385, 3909, 5163
Bpu10I CCTNAGC 3 cut(s) 1458, 3966, 5445
Bpu14I TTCGAA 1 cut(s) 316
BpuEI CTTGAG 5 cut(s) 647, 2585, 3119, 3344, 3686
BpuMI CCSGG 2 cut(s) 384, 4987
BsaAI YACGTR 3 cut(s) 157, 4625, 4952
BsaBI GATNNNNATC 2 cut(s) 494, 2850
BsaHI GRCGYC 2 cut(s) 347, 1128
BsaI GGTCTC 3 cut(s) 2063, 4796, 5172
BsaWI WCCGGW 6 cut(s) 406, 431, 3995, 4774, 5128, 5149
BsaXI ACNNNNNCTCC 8 cut(s) 551, 581, 635, 665, 2587, 2617, 4395, 4425
Bse118I RCCGGY 4 cut(s) 500, 807, 1813, 3753
Bse21I CCTNAGG 1 cut(s) 1488
Bse3DI GCAATG 4 cut(s) 531, 1059, 2498, 3081
Bse8I GATNNNNATC 2 cut(s) 494, 2850
BseBI CCWGG 8 cut(s) 2225, 3513, 4140, 4208, 4585, 4788, 5037, 5355
BseJI GATNNNNATC 2 cut(s) 494, 2850
BseMI GCAATG 4 cut(s) 531, 1059, 2498, 3081
BseRI GAGGAG 5 cut(s) 576, 656, 1088, 1979, 3912
BseX3I CGGCCG 1 cut(s) 809
BseYI CCCAGC 2 cut(s) 544, 4042
BsgI GTGCAG 5 cut(s) 325, 2103, 3348, 4482, 5268
Bsh1236I CGCG 2 cut(s) 2035, 2232
Bsh1285I CGRYCG 3 cut(s) 416, 498, 812
BshNI GGYRCC 1 cut(s) 3117
BsiEI CGRYCG 3 cut(s) 416, 498, 812
BsiHKAI GWGCWC 4 cut(s) 456, 858, 1616, 4983
BsiHKCI CYCGRG 1 cut(s) 3281
BslFI GGGAC 5 cut(s) 893, 2241, 2621, 4156, 5098
BsmBI CGTCTC 4 cut(s) 993, 1135, 3042, 4697
BsmFI GGGAC 5 cut(s) 893, 2241, 2621, 4156, 5098
BsmI GAATGC 2 cut(s) 1323, 1560
Bso31I GGTCTC 3 cut(s) 2063, 4796, 5172
BsoBI CYCGRG 1 cut(s) 3281
Bsp119I TTCGAA 1 cut(s) 316
Bsp1286I GDGCHC 4 cut(s) 456, 858, 1616, 4983
Bsp1407I TGTACA 3 cut(s) 2263, 2643, 4186
Bsp19I CCATGG 1 cut(s) 1022
BspACI CCGC 8 cut(s) 461, 513, 536, 944, 2035, 3311, 4163, 4728
BspFNI CGCG 2 cut(s) 2035, 2232
BspHI TCATGA 3 cut(s) 837, 1216, 1387
BspMAI CTGCAG 3 cut(s) 3273, 4006, 4726
BspMI ACCTGC 2 cut(s) 680, 3925
BspOI GCTAGC 1 cut(s) 3186
BspPI GGATC 1 cut(s) 3300
BspQI GCTCTTC 1 cut(s) 288
BspT104I TTCGAA 1 cut(s) 316
BspT107I GGYRCC 1 cut(s) 3117
BspTI CTTAAG 1 cut(s) 2066
BspTNI GGTCTC 3 cut(s) 2063, 4796, 5172
BsrDI GCAATG 4 cut(s) 531, 1059, 2498, 3081
BsrFI RCCGGY 4 cut(s) 500, 807, 1813, 3753
BsrGI TGTACA 3 cut(s) 2263, 2643, 4186
BssAI RCCGGY 4 cut(s) 500, 807, 1813, 3753
BssNI GRCGYC 2 cut(s) 347, 1128
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 8 cut(s) 39, 1022, 2576, 2706, 3635, 4374, 4564, 5045
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 8 cut(s) 2225, 3513, 4140, 4208, 4585, 4788, 5037, 5355
Bst6I CTCTTC 5 cut(s) 57, 273, 288, 991, 2471
BstACI GRCGYC 2 cut(s) 347, 1128
BstAFI CTTAAG 1 cut(s) 2066
BstAPI GCANNNNNTGC 4 cut(s) 830, 1268, 1753, 3268
BstAUI TGTACA 3 cut(s) 2263, 2643, 4186
BstBAI YACGTR 3 cut(s) 157, 4625, 4952
BstBI TTCGAA 1 cut(s) 316
BstDSI CCRYGG 2 cut(s) 303, 1022
BstENI CCTNNNNNAGG 2 cut(s) 1518, 1651
BstFNI CGCG 2 cut(s) 2035, 2232
BstHHI GCGC 1 cut(s) 3007
BstMCI CGRYCG 3 cut(s) 416, 498, 812
BstNI CCWGG 8 cut(s) 2225, 3513, 4140, 4208, 4585, 4788, 5037, 5355
BstNSI RCATGY 8 cut(s) 168, 1740, 2376, 2654, 2661, 3030, 4394, 5021
BstSFI CTRYAG 6 cut(s) 720, 2823, 3269, 4002, 4329, 4722
BstUI CGCG 2 cut(s) 2035, 2232
BstV2I GAAGAC 3 cut(s) 325, 476, 2807
BstX2I RGATCY 5 cut(s) 123, 608, 3292, 3427, 4931
BstXI CCANNNNNNTGG 2 cut(s) 2558, 3346
BstYI RGATCY 5 cut(s) 123, 608, 3292, 3427, 4931
BstZI CGGCCG 1 cut(s) 809
Bsu36I CCTNAGG 1 cut(s) 1488
BsuI GTATCC 3 cut(s) 540, 2288, 2431
BtgI CCRYGG 2 cut(s) 303, 1022
BtsI GCAGTG 3 cut(s) 4134, 4164, 4446
BveI ACCTGC 2 cut(s) 680, 3925
CaiI CAGNNNCTG 6 cut(s) 2054, 3455, 3536, 4049, 4541, 5354
CciI TCATGA 3 cut(s) 837, 1216, 1387
CfoI GCGC 1 cut(s) 3007
Cfr10I RCCGGY 4 cut(s) 500, 807, 1813, 3753
CseI GACGC 1 cut(s) 2238
CsiI ACCWGGT 1 cut(s) 3511
CspCI CAANNNNNGTGG 2 cut(s) 2115, 2150
DraI TTTAAA 1 cut(s) 4456
DraIII CACNNNGTG 1 cut(s) 1406
EaeI YGGCCR 2 cut(s) 809, 5038
EagI CGGCCG 1 cut(s) 809
Eam1104I CTCTTC 5 cut(s) 57, 273, 288, 991, 2471
EarI CTCTTC 5 cut(s) 57, 273, 288, 991, 2471
EciI GGCGGA 2 cut(s) 525, 4178
EclXI CGGCCG 1 cut(s) 809
Eco130I CCWWGG 8 cut(s) 39, 1022, 2576, 2706, 3635, 4374, 4564, 5045
Eco147I AGGCCT 1 cut(s) 1100
Eco31I GGTCTC 3 cut(s) 2063, 4796, 5172
Eco32I GATATC 3 cut(s) 1876, 3848, 5126
Eco52I CGGCCG 1 cut(s) 809
Eco57I CTGAAG 9 cut(s) 1536, 1565, 1752, 3636, 3943, 4370, 4446, 5262, 5343
Eco72I CACGTG 1 cut(s) 4625
Eco81I CCTNAGG 1 cut(s) 1488
Eco88I CYCGRG 1 cut(s) 3281
EcoNI CCTNNNNNAGG 2 cut(s) 1518, 1651
EcoO109I RGGNCCY 4 cut(s) 215, 646, 1644, 1825
EcoRI GAATTC 2 cut(s) 1942, 5288
EcoRII CCWGG 8 cut(s) 2223, 3511, 4138, 4206, 4583, 4786, 5035, 5353
EcoRV GATATC 3 cut(s) 1876, 3848, 5126
EcoT14I CCWWGG 8 cut(s) 39, 1022, 2576, 2706, 3635, 4374, 4564, 5045
EcoT22I ATGCAT 3 cut(s) 667, 2026, 2199
ErhI CCWWGG 8 cut(s) 39, 1022, 2576, 2706, 3635, 4374, 4564, 5045
Esp3I CGTCTC 4 cut(s) 993, 1135, 3042, 4697
FalI AAGNNNNNCTT 6 cut(s) 2690, 2722, 4358, 4390, 4882, 4914
FaqI GGGAC 5 cut(s) 893, 2241, 2621, 4156, 5098
FauI CCCGC 1 cut(s) 951
FbaI TGATCA 3 cut(s) 2911, 3163, 4054
FblI GTMKAC 3 cut(s) 365, 1125, 3661
GlaI GCGC 1 cut(s) 3006
GsaI CCCAGC 2 cut(s) 548, 4046
GsuI CTGGAG 6 cut(s) 897, 2744, 2823, 3385, 3909, 5163
HgaI GACGC 1 cut(s) 2238
HhaI GCGC 1 cut(s) 3007
Hin1I GRCGYC 2 cut(s) 347, 1128
Hin6I GCGC 1 cut(s) 3005
HinP1I GCGC 1 cut(s) 3005
HincII GTYRAC 4 cut(s) 328, 1126, 1543, 4095
HindII GTYRAC 4 cut(s) 328, 1126, 1543, 4095
HindIII AAGCTT 2 cut(s) 3342, 3955
HpaI GTTAAC 1 cut(s) 1543
Hpy99I CGWCG 8 cut(s) 278, 338, 349, 352, 364, 452, 718, 1130
Hsp92I GRCGYC 2 cut(s) 347, 1128
HspAI GCGC 1 cut(s) 3005
KpnI GGTACC 1 cut(s) 3121
KroI GCCGGC 2 cut(s) 500, 807
KroNI GCCGGC 2 cut(s) 502, 809
Ksp22I TGATCA 3 cut(s) 2911, 3163, 4054
KspAI GTTAAC 1 cut(s) 1543
LguI GCTCTTC 1 cut(s) 288
MabI ACCWGGT 1 cut(s) 3511
MfeI CAATTG 1 cut(s) 4146
MflI RGATCY 5 cut(s) 123, 608, 3292, 3427, 4931
MhlI GDGCHC 4 cut(s) 456, 858, 1616, 4983
MlsI TGGCCA 1 cut(s) 5040
MluI ACGCGT 1 cut(s) 2230
MluNI TGGCCA 1 cut(s) 5040
MlyI GAGTC 6 cut(s) 205, 437, 493, 527, 3344, 5204
MmeI TCCRAC 3 cut(s) 4381, 5195, 5209
Mox20I TGGCCA 1 cut(s) 5040
Mph1103I ATGCAT 3 cut(s) 667, 2026, 2199
MreI CGCCGGCG 1 cut(s) 500
MroNI GCCGGC 2 cut(s) 500, 807
MroXI GAANNNNTTC 1 cut(s) 845
MscI TGGCCA 1 cut(s) 5040
MslI CAYNNNNRTG 3 cut(s) 3417, 4395, 4974
Msp20I TGGCCA 1 cut(s) 5040
MspA1I CMGCKG 3 cut(s) 1367, 4538, 5024
MspCI CTTAAG 1 cut(s) 2066
MunI CAATTG 1 cut(s) 4146
Mva1269I GAATGC 2 cut(s) 1323, 1560
MvaI CCWGG 8 cut(s) 2225, 3513, 4140, 4208, 4585, 4788, 5037, 5355
MvnI CGCG 2 cut(s) 2035, 2232
NaeI GCCGGC 2 cut(s) 502, 809
NciI CCSGG 2 cut(s) 384, 4987
NcoI CCATGG 1 cut(s) 1022
NgoMIV GCCGGC 2 cut(s) 500, 807
NheI GCTAGC 1 cut(s) 3182
NmeAIII GCCGAG 1 cut(s) 564
NmuCI GTSAC 6 cut(s) 1948, 2070, 3756, 4228, 4243, 4620
NsiI ATGCAT 3 cut(s) 667, 2026, 2199
NspI RCATGY 8 cut(s) 168, 1740, 2376, 2654, 2661, 3030, 4394, 5021
NspV TTCGAA 1 cut(s) 316
OliI CACNNNNGTG 1 cut(s) 4974
PaeI GCATGC 1 cut(s) 4394
PagI TCATGA 3 cut(s) 837, 1216, 1387
PasI CCCWGGG 1 cut(s) 2224
PceI AGGCCT 1 cut(s) 1100
PciI ACATGT 2 cut(s) 164, 3026
PciSI GCTCTTC 1 cut(s) 288
PcsI WCGNNNNNNNCGW 4 cut(s) 356, 447, 1008, 4948
PctI GAATGC 2 cut(s) 1323, 1560
PdiI GCCGGC 2 cut(s) 502, 809
PdmI GAANNNNTTC 1 cut(s) 845
PfeI GAWTC 9 cut(s) 490, 1196, 2045, 2690, 3653, 4355, 4421, 4598, 5155
PflFI GACNNNGTC 1 cut(s) 332
PflMI CCANNNNNTGG 4 cut(s) 2171, 2287, 4667, 4763
Ple19I CGATCG 1 cut(s) 498
PleI GAGTC 6 cut(s) 204, 436, 492, 526, 3343, 5203
PmaCI CACGTG 1 cut(s) 4625
PmlI CACGTG 1 cut(s) 4625
PpsI GAGTC 6 cut(s) 204, 436, 492, 526, 3343, 5203
Ppu21I YACGTR 3 cut(s) 157, 4625, 4952
PpuMI RGGWCCY 3 cut(s) 215, 646, 1644
PscI ACATGT 2 cut(s) 164, 3026
PshBI ATTAAT 1 cut(s) 2889
Psp1406I AACGTT 1 cut(s) 5307
Psp5II RGGWCCY 3 cut(s) 215, 646, 1644
Psp6I CCWGG 8 cut(s) 2223, 3511, 4138, 4206, 4583, 4786, 5035, 5353
PspCI CACGTG 1 cut(s) 4625
PspFI CCCAGC 2 cut(s) 544, 4042
PspGI CCWGG 8 cut(s) 2223, 3511, 4138, 4206, 4583, 4786, 5035, 5353
PspPPI RGGWCCY 3 cut(s) 215, 646, 1644
PstI CTGCAG 3 cut(s) 3273, 4006, 4726
PstNI CAGNNNCTG 6 cut(s) 2054, 3455, 3536, 4049, 4541, 5354
PsuI RGATCY 5 cut(s) 123, 608, 3292, 3427, 4931
PsyI GACNNNGTC 1 cut(s) 332
PvuI CGATCG 1 cut(s) 498
PvuII CAGCTG 3 cut(s) 1367, 4538, 5024
RseI CAYNNNNRTG 3 cut(s) 3417, 4395, 4974
SalI GTCGAC 1 cut(s) 1124
SapI GCTCTTC 1 cut(s) 288
ScaI AGTACT 2 cut(s) 1662, 2829
SchI GAGTC 6 cut(s) 205, 437, 493, 527, 3344, 5204
SduI GDGCHC 4 cut(s) 456, 858, 1616, 4983
SexAI ACCWGGT 1 cut(s) 3511
SfcI CTRYAG 6 cut(s) 720, 2823, 3269, 4002, 4329, 4722
SfuI TTCGAA 1 cut(s) 316
SgrAI CRCCGGYG 1 cut(s) 500
SmiMI CAYNNNNRTG 3 cut(s) 3417, 4395, 4974
SmlI CTYRAG 6 cut(s) 626, 2066, 2564, 3134, 3323, 3665
SmoI CTYRAG 6 cut(s) 626, 2066, 2564, 3134, 3323, 3665
SphI GCATGC 1 cut(s) 4394
SseBI AGGCCT 1 cut(s) 1100
SsiI CCGC 8 cut(s) 461, 513, 536, 944, 2035, 3311, 4163, 4728
SspI AATATT 2 cut(s) 2383, 4831
StuI AGGCCT 1 cut(s) 1100
StyI CCWWGG 8 cut(s) 39, 1022, 2576, 2706, 3635, 4374, 4564, 5045
TaqII GACCGA 1 cut(s) 402
TatI WGTACW 7 cut(s) 1660, 2263, 2643, 2827, 4186, 4405, 5278
TauI GCSGC 4 cut(s) 516, 2038, 3314, 4730
TfiI GAWTC 9 cut(s) 490, 1196, 2045, 2690, 3653, 4355, 4421, 4598, 5155
TseFI GTSAC 6 cut(s) 1948, 2070, 3756, 4228, 4243, 4620
Tsp45I GTSAC 6 cut(s) 1948, 2070, 3756, 4228, 4243, 4620
TspGWI ACGGA 4 cut(s) 305, 609, 3382, 4850
Tth111I GACNNNGTC 1 cut(s) 332
Van91I CCANNNNNTGG 4 cut(s) 2171, 2287, 4667, 4763
Vha464I CTTAAG 1 cut(s) 2066
VspI ATTAAT 1 cut(s) 2889
XagI CCTNNNNNAGG 2 cut(s) 1518, 1651
XapI RAATTY 8 cut(s) 863, 1627, 1942, 3264, 3559, 3589, 4191, 5288
XbaI TCTAGA 1 cut(s) 955
XceI RCATGY 8 cut(s) 168, 1740, 2376, 2654, 2661, 3030, 4394, 5021
XcmI CCANNNNNNNNNTGG 2 cut(s) 2704, 5415
XmaJI CCTAGG 2 cut(s) 3635, 4564
XmiI GTMKAC 3 cut(s) 365, 1125, 3661
XmnI GAANNNNTTC 1 cut(s) 845
ZraI GACGTC 2 cut(s) 348, 1129
ZrmI AGTACT 2 cut(s) 1662, 2829
Zsp2I ATGCAT 3 cut(s) 667, 2026, 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.